##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364486_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2045015 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.16009613621416 31.0 28.0 31.0 22.0 34.0 2 28.405837120999113 31.0 28.0 31.0 22.0 34.0 3 28.46177754197402 31.0 28.0 33.0 22.0 34.0 4 31.81633093155796 35.0 32.0 37.0 23.0 37.0 5 30.96834742043457 35.0 31.0 37.0 16.0 37.0 6 30.98962794893925 35.0 31.0 37.0 16.0 37.0 7 30.566692175852012 35.0 30.0 37.0 11.0 37.0 8 30.56928042092601 35.0 30.0 37.0 10.0 37.0 9 31.130616156849705 37.0 30.0 39.0 10.0 39.0 10-11 31.240855935042042 37.0 30.0 39.0 10.0 39.0 12-13 31.02201744241485 36.0 30.0 39.0 8.0 39.0 14-15 31.815640227577795 37.0 30.0 40.0 8.0 41.0 16-17 31.866801954997882 37.0 30.0 40.0 8.0 41.0 18-19 31.769186778581087 37.0 30.0 40.0 7.5 41.0 20-21 31.720492759221813 37.0 30.0 40.0 4.5 41.0 22-23 31.65587660726205 37.0 30.0 40.0 2.0 41.0 24-25 31.4905130280218 37.0 29.5 40.0 2.0 41.0 26-27 31.49965232528857 37.0 30.0 40.0 2.0 41.0 28-29 31.354226252619174 37.0 30.0 40.0 2.0 41.0 30-31 30.893287090803735 36.5 28.5 40.0 2.0 41.0 32-33 30.745989149223846 36.0 28.0 40.0 2.0 41.0 34-35 30.558367787033347 36.0 28.0 40.0 2.0 41.0 36-37 30.459948460035747 36.0 28.0 40.0 2.0 41.0 38-39 30.24776982075926 36.0 27.0 40.0 2.0 41.0 40-41 29.99593425965091 35.0 27.0 40.0 2.0 41.0 42-43 29.847382537536397 35.0 26.5 40.0 2.0 41.0 44-45 29.69183086676626 35.0 26.0 40.0 2.0 41.0 46-47 29.481540477698207 35.0 26.0 40.0 2.0 41.0 48-49 29.20649115043166 35.0 25.0 40.0 2.0 41.0 50-51 28.901561602237635 35.0 24.0 40.0 2.0 41.0 52-53 28.776406774522435 35.0 24.0 40.0 2.0 41.0 54-55 28.35250205988709 35.0 23.0 39.0 2.0 41.0 56-57 28.336561345515804 34.5 23.0 39.0 2.0 41.0 58-59 28.118151456101792 34.0 22.5 39.0 2.0 41.0 60-61 27.868519301814413 34.0 22.0 39.0 2.0 41.0 62-63 27.631640843710194 34.0 22.0 39.0 2.0 40.0 64-65 27.246213352958293 34.0 20.0 38.0 2.0 40.0 66-67 26.90235817341193 33.0 18.5 38.0 2.0 40.0 68-69 26.744744170580656 33.0 18.5 37.5 2.0 40.0 70-71 26.747333149145604 33.0 20.0 37.0 2.0 40.0 72-73 26.536670146673742 33.0 18.5 37.0 2.0 39.0 74-75 26.204962066292914 33.0 17.5 36.0 2.0 39.0 76-77 26.062620323078313 33.0 18.0 36.0 2.0 39.0 78-79 25.709038075515338 33.0 15.5 35.5 2.0 38.5 80-81 25.445326562396854 33.0 13.0 35.0 2.0 37.0 82-83 25.288142385263676 32.0 13.0 35.0 2.0 37.0 84-85 24.94809451275419 32.0 9.0 35.0 2.0 36.0 86-87 24.778222409126585 32.0 9.0 35.0 2.0 36.0 88-89 24.658828908345416 32.0 7.5 35.0 2.0 36.0 90-91 24.491026716185456 32.0 7.0 35.0 2.0 35.0 92-93 24.4650777622658 32.0 7.0 35.0 2.0 35.0 94-95 24.429198807832705 32.0 7.0 35.0 2.0 35.0 96-97 24.318780546841957 32.0 6.0 35.0 2.0 35.0 98-99 24.205313408459105 32.0 2.0 35.0 2.0 35.0 100-101 24.104735417588625 32.0 2.0 35.0 2.0 35.0 102-103 23.013168118571258 30.0 2.0 34.0 2.0 35.0 104-105 23.8876120713051 31.5 2.0 34.5 2.0 35.0 106-107 24.014850746816037 32.0 2.0 35.0 2.0 35.0 108-109 24.0653782490593 32.0 2.0 35.0 2.0 35.0 110-111 24.000792659222547 32.0 2.0 35.0 2.0 35.0 112-113 23.933062838169892 32.0 2.0 35.0 2.0 35.0 114-115 23.873476967161608 32.0 2.0 35.0 2.0 35.0 116-117 23.784940941753483 32.0 2.0 35.0 2.0 35.0 118-119 23.662625213017996 32.0 2.0 35.0 2.0 35.0 120-121 23.49217414053198 32.0 2.0 35.0 2.0 35.0 122-123 23.179548805265487 32.0 2.0 35.0 2.0 35.0 124-125 22.437750334349627 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5765808658465374 1101 2 1.5298058896855267 1101 3 1.6124597549881123 1101 4 1.760107808261214 1101 5 1.9752934623775822 1101 6 1.9145302747624093 1101 7 2.416144799997223 1101 8 2.3018537880074135 1101 9 3.0096214844978952 1101 10-11 2.752954865585423 1101 12-13 2.8534967399311704 1101 14-15 2.856972117727569 1101 16-17 2.86334131927541 1101 18-19 2.891671608229302 1101 20-21 3.2113380121286355 1101 22-23 3.229509941165624 1101 24-25 3.242174595953081 1101 26-27 3.0888743538385306 1101 28-29 3.132987355900749 1101 30-31 3.5577315535572644 1101 32-33 3.487702627351826 1101 34-35 3.544250427625613 1101 36-37 3.563301294519757 1101 38-39 3.6788897116717827 1101 40-41 3.7769399513591217 1101 42-43 3.695124796152399 1101 44-45 3.60240947239593 1101 46-47 3.454552886038357 1101 48-49 3.5420003411092615 1101 50-51 3.781761209538441 1101 52-53 3.7430285860684585 1101 54-55 4.090012216570244 1101 56-57 3.639856657857788 1101 58-59 3.718674774638469 1101 60-61 3.868684483539255 1101 62-63 3.889581719188026 1101 64-65 3.9610195852748618 1101 66-67 4.130082777689633 1101 68-69 4.082316145730427 1101 70-71 3.7400423372869973 1101 72-73 3.910239472214748 1101 74-75 3.973615365822951 1101 76-77 3.793085945470846 1101 78-79 3.68894833423019 1101 80-81 3.7749265478537843 1101 82-83 3.6079614928675525 1101 84-85 3.8441343604855653 1101 86-87 3.7364537074932507 1101 88-89 3.59554283579606 1101 90-91 3.682797688689231 1101 92-93 3.5537861976567875 1101 94-95 3.4012758478724585 1101 96-97 3.333549482420029 1101 98-99 3.418480268422126 1101 100-101 3.6190658703015295 1101 102-103 3.3226543414657357 1101 104-105 3.5012646923956545 1101 106-107 3.6035483380627618 1101 108-109 3.604497507756701 1101 110-111 3.5203038753927594 1101 112-113 3.5254588680813477 1101 114-115 3.5079766623105044 1101 116-117 3.454862288409931 1101 118-119 3.42405420233899 1101 120-121 3.348447408261084 1101 122-123 3.4157423360684724 1101 124-125 3.0997938157808704 1102 1 0.8297138885174888 1102 2 -1.232218108147027 1102 3 0.46202389843965364 1102 4 0.9869754406656632 1102 5 1.3632718320729431 1102 6 0.9895081392664693 1102 7 1.8335151920847643 1102 8 1.5310169612353377 1102 9 2.3291149972934058 1102 10-11 2.2067864335973795 1102 12-13 2.1400016172166865 1102 14-15 2.22738118738879 1102 16-17 2.264098287372878 1102 18-19 2.229832776000869 1102 20-21 2.4141151401713046 1102 22-23 2.3334768854916987 1102 24-25 2.505062403255529 1102 26-27 2.3224126118435287 1102 28-29 2.274940555748227 1102 30-31 2.5895670783669935 1102 32-33 2.3402889824874187 1102 34-35 2.486240973890851 1102 36-37 1.8780100868063876 1102 38-39 1.8950782267048716 1102 40-41 2.130495746871638 1102 42-43 1.9847388606210785 1102 44-45 1.916548242285394 1102 46-47 1.7852600078827408 1102 48-49 1.654706738810514 1102 50-51 1.7676658027918073 1102 52-53 1.6407143898370684 1102 54-55 1.9806849398361344 1102 56-57 1.6612923267841104 1102 58-59 1.6648209554243252 1102 60-61 1.7693118250332631 1102 62-63 2.002038298192076 1102 64-65 2.1661085242200535 1102 66-67 2.2246270677285374 1102 68-69 2.0500876468890894 1102 70-71 1.591375682724987 1102 72-73 1.6917518561658298 1102 74-75 1.8819139605794248 1102 76-77 1.8572033720071666 1102 78-79 1.6913926910939026 1102 80-81 1.7226303839966448 1102 82-83 1.5069810514962647 1102 84-85 1.491972558287717 1102 86-87 1.5874178253826123 1102 88-89 1.6992070213646784 1102 90-91 1.5539269496609442 1102 92-93 1.5831010350788226 1102 94-95 1.6405424553687276 1102 96-97 1.5747729852434453 1102 98-99 1.439306468020039 1102 100-101 1.3091089023464662 1102 102-103 1.0643431614293029 1102 104-105 1.3478959849911334 1102 106-107 1.579638442258915 1102 108-109 1.5282311028353774 1102 110-111 1.4605760592183046 1102 112-113 1.3941183880178905 1102 114-115 1.6811792012972795 1102 116-117 1.348113160568051 1102 118-119 1.0269264231193347 1102 120-121 1.361726000076839 1102 122-123 1.6539441256347587 1102 124-125 1.7017160704364436 1103 1 1.468488871581183 1103 2 1.30109853070147 1103 3 1.2267392807594781 1103 4 1.3947362266104797 1103 5 1.7443488842854826 1103 6 1.719248145951429 1103 7 2.025813374212998 1103 8 1.9658341333400742 1103 9 2.668074801917779 1103 10-11 2.479597172235188 1103 12-13 2.452412211264491 1103 14-15 2.246649309771545 1103 16-17 2.363233613368525 1103 18-19 2.5215086049304105 1103 20-21 2.651298916714552 1103 22-23 2.5560952972156628 1103 24-25 2.9054094049296353 1103 26-27 2.537939593319461 1103 28-29 2.6551487782477388 1103 30-31 3.263138884892964 1103 32-33 3.0515728062839393 1103 34-35 3.1108390792459417 1103 36-37 3.0263247857270876 1103 38-39 3.2573347908437533 1103 40-41 3.3018222526622303 1103 42-43 3.1939438269795417 1103 44-45 3.1687765440228013 1103 46-47 3.1422628964585186 1103 48-49 3.115608076196377 1103 50-51 3.2417363660085563 1103 52-53 3.1551335167011807 1103 54-55 3.3636125362852987 1103 56-57 3.180386410953691 1103 58-59 3.312926220957987 1103 60-61 3.3376697576541794 1103 62-63 3.4678278237283706 1103 64-65 3.5359301619203585 1103 66-67 3.813755375592031 1103 68-69 3.8238391519900397 1103 70-71 3.6344346282282416 1103 72-73 3.577619365724857 1103 74-75 3.4796597752255067 1103 76-77 3.313960285619654 1103 78-79 3.3406120448023913 1103 80-81 3.3871786065176472 1103 82-83 3.3293366146467385 1103 84-85 3.4038166504283787 1103 86-87 3.294781776974336 1103 88-89 3.2877055602390044 1103 90-91 3.3008873532805687 1103 92-93 3.1265965091180057 1103 94-95 3.1582206251086475 1103 96-97 3.013555097497015 1103 98-99 3.2456568207716394 1103 100-101 3.1570213573277783 1103 102-103 2.856117258538898 1103 104-105 2.968010510590119 1103 106-107 2.914088801423201 1103 108-109 2.9178704195652223 1103 110-111 2.9236365022096855 1103 112-113 2.978386845365094 1103 114-115 2.8186569942290625 1103 116-117 2.9229820855757787 1103 118-119 2.9915995318509445 1103 120-121 3.005600324281584 1103 122-123 3.131672751409969 1103 124-125 2.9460231973693425 1104 1 1.1592872885923882 1104 2 1.1653857141675807 1104 3 1.14698038798862 1104 4 1.2363698127455827 1104 5 1.5200583939648489 1104 6 1.7045751776310034 1104 7 2.0663856299090178 1104 8 1.9251516034977314 1104 9 2.548312695443226 1104 10-11 2.0030262844865305 1104 12-13 2.051393120617977 1104 14-15 2.225742975467046 1104 16-17 2.1387533282283506 1104 18-19 1.9866300225874411 1104 20-21 2.2432946095844812 1104 22-23 2.4091564698462804 1104 24-25 2.420129873324889 1104 26-27 2.506511735590639 1104 28-29 2.3847384207519617 1104 30-31 2.8601201678086845 1104 32-33 2.81769603373834 1104 34-35 2.880279777679924 1104 36-37 2.795453120655285 1104 38-39 2.853802819526269 1104 40-41 3.064453865027094 1104 42-43 2.965331138069139 1104 44-45 2.8544942338520407 1104 46-47 2.7939801217664773 1104 48-49 2.335970101424227 1104 50-51 2.853858802913763 1104 52-53 2.69947136052107 1104 54-55 3.0592471252986932 1104 56-57 2.967764877371824 1104 58-59 3.0723284104271897 1104 60-61 3.1122130326196142 1104 62-63 3.1518880109464718 1104 64-65 3.432753389792566 1104 66-67 3.3287812554273195 1104 68-69 3.4717695905105934 1104 70-71 3.2519000530025366 1104 72-73 3.3273203412811867 1104 74-75 3.4554512096914642 1104 76-77 3.3311696277987544 1104 78-79 3.2220894950750854 1104 80-81 3.1682358985942045 1104 82-83 3.130229759889364 1104 84-85 3.237185976969098 1104 86-87 3.1388657094353256 1104 88-89 3.0464524747033224 1104 90-91 3.1295873231503855 1104 92-93 2.8339639590804246 1104 94-95 2.8550487091528893 1104 96-97 2.8887734968136733 1104 98-99 2.945912307475485 1104 100-101 2.935191213348787 1104 102-103 2.8031288188995767 1104 104-105 2.8466050796089597 1104 106-107 2.950239235084844 1104 108-109 2.910863833184983 1104 110-111 2.90668407824354 1104 112-113 2.943747715454137 1104 114-115 2.757952617776965 1104 116-117 2.850583375632606 1104 118-119 2.8317520197623978 1104 120-121 2.810611284230358 1104 122-123 2.8853627400996764 1104 124-125 2.6032085819764568 1105 1 1.0374281476227658 1105 2 1.0162318826325816 1105 3 0.876591747728348 1105 4 1.176377591892713 1105 5 1.4477402755111193 1105 6 1.5398266602338673 1105 7 1.924052917219111 1105 8 1.6472621798959288 1105 9 2.350415477087761 1105 10-11 2.112783658945631 1105 12-13 2.2553381541262674 1105 14-15 2.218070520266483 1105 16-17 2.1725973002157595 1105 18-19 2.4195179951363954 1105 20-21 2.577098621754459 1105 22-23 2.5755931529728713 1105 24-25 2.670473092900089 1105 26-27 2.6178330264977596 1105 28-29 2.5833023572756595 1105 30-31 2.8432415254124415 1105 32-33 2.7632998543877854 1105 34-35 2.8706073807910357 1105 36-37 2.716928603395491 1105 38-39 2.78286059427165 1105 40-41 2.88264272549554 1105 42-43 2.908975877770267 1105 44-45 2.823406730222633 1105 46-47 2.5863004095282385 1105 48-49 2.6588480865016884 1105 50-51 2.7539932035352983 1105 52-53 2.7051393694204826 1105 54-55 2.8833585939649247 1105 56-57 2.7781376316006465 1105 58-59 2.735587709916622 1105 60-61 2.5450004139718345 1105 62-63 2.4713177681066973 1105 64-65 2.8333246669198253 1105 66-67 3.034149711120481 1105 68-69 3.0052659269834656 1105 70-71 2.920751705177473 1105 72-73 3.0390153660817383 1105 74-75 2.962237568532043 1105 76-77 2.881409184527829 1105 78-79 2.9108667168583153 1105 80-81 2.8799536947446533 1105 82-83 2.8332643650345126 1105 84-85 3.0039673800261717 1105 86-87 2.9357228871740837 1105 88-89 2.774780587296796 1105 90-91 2.7977759459739 1105 92-93 2.730521236012887 1105 94-95 2.645316986158349 1105 96-97 2.7554644812957427 1105 98-99 2.699027637410712 1105 100-101 2.6951830298505826 1105 102-103 2.5133067113806398 1105 104-105 2.2321554947037114 1105 106-107 2.384556269452059 1105 108-109 2.407210982343212 1105 110-111 2.5005835839491795 1105 112-113 2.599964673498313 1105 114-115 2.396160984992438 1105 116-117 2.496531093322634 1105 118-119 2.6524426074129046 1105 120-121 2.651544608795657 1105 122-123 2.7269904134898475 1105 124-125 2.5524256696257943 1106 1 0.9074772643123552 1106 2 0.8508902362870536 1106 3 0.9209111750546128 1106 4 0.8379479111818107 1106 5 0.929077858685563 1106 6 1.137342286779436 1106 7 1.1943729218160328 1106 8 1.0386142877784152 1106 9 1.698859984935762 1106 10-11 1.4169319011063166 1106 12-13 1.3334986169282708 1106 14-15 1.4390073657796059 1106 16-17 1.4361675680181527 1106 18-19 1.5958249809743847 1106 20-21 1.5297467531597277 1106 22-23 1.3104927048541697 1106 24-25 1.1919502020826513 1106 26-27 1.2975323072846088 1106 28-29 1.3486294796507714 1106 30-31 1.7326800688433543 1106 32-33 1.5285279865734687 1106 34-35 1.436499293093405 1106 36-37 1.469861519691463 1106 38-39 1.6905035958164731 1106 40-41 2.000385839167773 1106 42-43 1.997880464283746 1106 44-45 1.8072230144628598 1106 46-47 1.8298249426829045 1106 48-49 1.8107598548197963 1106 50-51 1.9397303326531024 1106 52-53 1.5813803150821713 1106 54-55 2.1341275935716943 1106 56-57 1.8865052236288804 1106 58-59 1.835667713463156 1106 60-61 2.1047141434906607 1106 62-63 2.0802849072732563 1106 64-65 2.1363543641979383 1106 66-67 2.3672489692915413 1106 68-69 2.1836618861183688 1106 70-71 1.998119140016371 1106 72-73 1.9035804852878329 1106 74-75 2.2225562815356597 1106 76-77 1.799069262986773 1106 78-79 1.7414320253407354 1106 80-81 1.8564787097941853 1106 82-83 1.6868069782825685 1106 84-85 1.8717610399184572 1106 86-87 1.9245832084024137 1106 88-89 1.664163181354624 1106 90-91 1.8070221976425174 1106 92-93 1.666208556542454 1106 94-95 1.6502933085459368 1106 96-97 1.5504833864303151 1106 98-99 1.4895212820118822 1106 100-101 1.304624798207783 1106 102-103 1.0107518867116632 1106 104-105 1.359272728519496 1106 106-107 1.2849529235836563 1106 108-109 1.3514671872467083 1106 110-111 1.2520109568314304 1106 112-113 1.0974930905795368 1106 114-115 1.165390400952532 1106 116-117 1.4850539848777018 1106 118-119 1.194920241863084 1106 120-121 1.1121775677046237 1106 122-123 1.3880442429828612 1106 124-125 1.5298852641835161 1107 1 0.6453755445204159 1107 2 0.7833309035303557 1107 3 0.5193761618913975 1107 4 0.5920993668071439 1107 5 0.8057565897427814 1107 6 1.1356880248374495 1107 7 1.1697721014968643 1107 8 1.1858416807158143 1107 9 1.8292000213539445 1107 10-11 1.3475953944612016 1107 12-13 1.591653791935908 1107 14-15 1.482762252403031 1107 16-17 1.6034785272558487 1107 18-19 1.636532795586156 1107 20-21 1.745545649709186 1107 22-23 1.6237485259693258 1107 24-25 2.0003281353142413 1107 26-27 2.0110815043558894 1107 28-29 1.6257069891298848 1107 30-31 1.9812907721892152 1107 32-33 1.7612381615861032 1107 34-35 1.846880203397479 1107 36-37 1.806365668939204 1107 38-39 1.8855990290136937 1107 40-41 2.260851152080239 1107 42-43 2.1786549854090467 1107 44-45 2.058444089549578 1107 46-47 2.096191065769794 1107 48-49 1.9768413874092765 1107 50-51 2.339127115685372 1107 52-53 1.698461719652773 1107 54-55 2.329263114264098 1107 56-57 2.0715297104071873 1107 58-59 1.9914314389952885 1107 60-61 2.2296493948903233 1107 62-63 2.2108469608342958 1107 64-65 2.354751921103176 1107 66-67 2.356465932261372 1107 68-69 2.2720638749189064 1107 70-71 2.2153272425746238 1107 72-73 2.01213657748508 1107 74-75 2.1942750528585364 1107 76-77 1.882570248317439 1107 78-79 1.9952064895602248 1107 80-81 1.9764609284558041 1107 82-83 1.4487562580784648 1107 84-85 1.8822937220936602 1107 86-87 1.8412613880645807 1107 88-89 1.5791965449788492 1107 90-91 1.4063958343700982 1107 92-93 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2.19541334767575 2204 50-51 2.422193270328801 2204 52-53 2.3245802522848926 2204 54-55 2.12805074946937 2204 56-57 2.1691978799324723 2204 58-59 1.999445767070263 2204 60-61 2.332966192009131 2204 62-63 2.0766959554807407 2204 64-65 2.4975258377755836 2204 66-67 2.8013098949400472 2204 68-69 2.8852856944020964 2204 70-71 2.6475198873840213 2204 72-73 2.662443030237675 2204 74-75 2.805945136275234 2204 76-77 2.632219378060366 2204 78-79 2.6671296701426783 2204 80-81 2.533105564374626 2204 82-83 2.522719295220849 2204 84-85 2.7173153680668243 2204 86-87 2.4423952588236553 2204 88-89 2.4406590344626977 2204 90-91 2.5103992514643316 2204 92-93 2.350955023945364 2204 94-95 2.3127036713545195 2204 96-97 2.375619419827725 2204 98-99 2.469543932460013 2204 100-101 2.4364343070247614 2204 102-103 2.362198745511918 2204 104-105 2.278765356195759 2204 106-107 2.310758485142923 2204 108-109 2.333829736410653 2204 110-111 2.438172327274966 2204 112-113 2.318365744234672 2204 114-115 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-10.293436332345223 2216 100-101 -10.631613736225635 2216 102-103 -10.292834750706795 2216 104-105 -10.145348285672494 2216 106-107 -10.174465721642143 2216 108-109 -10.59663443827867 2216 110-111 -10.229143115805527 2216 112-113 -10.34386881173753 2216 114-115 -10.319783834074357 2216 116-117 -10.191688278755489 2216 118-119 -10.353034476196402 2216 120-121 -10.46317695218573 2216 122-123 -10.711208797463208 2216 124-125 -10.319364910620639 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 137326.0 3 40598.0 4 19314.0 5 15134.0 6 16199.0 7 18537.0 8 20235.0 9 20930.0 10 21911.0 11 23893.0 12 25321.0 13 27221.0 14 28188.0 15 22838.0 16 16512.0 17 14250.0 18 12723.0 19 11525.0 20 11455.0 21 12260.0 22 14805.0 23 19684.0 24 27351.0 25 36173.0 26 42420.0 27 47533.0 28 52642.0 29 59844.0 30 66869.0 31 77035.0 32 89271.0 33 105491.0 34 126795.0 35 159771.0 36 220402.0 37 281481.0 38 100631.0 39 447.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.558390168339294 21.19611686598685 15.021375835395057 23.224117130278803 2 16.28344911929483 22.332371153378787 37.85733950315133 23.526840224175054 3 19.801077253712076 25.94357498600255 29.929462620078585 24.325885140206797 4 13.272569363653597 18.77997561538337 36.403029022004446 31.544425998958587 5 12.668317836299488 39.59790025990029 33.999897311266665 13.733884592533553 6 30.441175679567806 38.65359291114531 17.052801131337553 13.852430277949335 7 26.635431737544423 33.88999592077499 21.193236899280315 18.281335442400266 8 25.354826248218227 36.87987618672723 19.400591193707626 18.36470637134691 9 25.676095285364653 16.437581142436606 20.11344660063618 37.77287697156256 10-11 24.421144871666666 28.00269827993212 27.083899244330027 20.492257604071185 12-13 25.238446616564147 25.352382656880877 27.944647622233077 21.46452310432189 14-15 22.148766998694384 26.355005598994623 26.489405919775454 25.006821482535535 16-17 21.22068331154799 29.688457387529198 27.02969212495348 22.061167175969327 18-19 21.110517520235405 27.700737288767563 30.021381496509157 21.16736369448788 20-21 22.084187054088815 26.776040724313805 30.184648185547953 20.955124036049426 22-23 21.515056160325205 26.853483886379102 30.1058903121208 21.5255696411749 24-25 21.33803662336246 26.900442382129846 30.207562379841278 21.55395861466641 26-27 21.257193602122683 27.08816482673618 30.23705359445994 21.4175879766812 28-29 21.104566340558282 27.092244915523395 30.36233459145823 21.440854152460094 30-31 21.2171482514805 26.944381214390305 30.246139183760988 21.5923313503682 32-33 21.199585919705193 26.97230923602479 30.13412133119871 21.693983513071306 34-35 21.454002844015342 27.03985729067441 30.027442596464738 21.478697268845515 36-37 21.488761950215515 26.817529338098467 30.039078344709484 21.654630366976537 38-39 21.425937833892828 27.034432678648706 30.056381280241368 21.4832482072171 40-41 21.538856499473717 27.022177342948687 29.91226129191183 21.526704865665767 42-43 21.529444441184467 27.007502185814797 29.63397626606109 21.829077106939643 44-45 21.57499070285897 27.196796453369913 29.438941683392557 21.789271160378558 46-47 21.688028389061586 27.36327348530303 28.842261638434575 22.106436487200813 48-49 21.823048198435757 27.519731522686026 28.53786611292976 22.119354165948458 50-51 21.79826976002966 27.687508086119216 28.246254338378744 22.267967815472375 52-53 21.96939042211702 27.706174066821564 28.179373876993967 22.145061634067453 54-55 21.767526394309414 27.546488405011576 28.148699245149434 22.537285955529573 56-57 21.84341396863585 27.697933017442455 28.172136077574194 22.2865169363475 58-59 21.904435908783064 27.590971215370057 28.201211238059376 22.3033816377875 60-61 21.990278799911003 27.562951860988793 28.30033031542556 22.146439023674645 62-63 21.905146581333668 27.495589259308524 28.362529633135907 22.236734526221902 64-65 21.826892549458538 27.40355945909058 28.263901492378146 22.505646499072736 66-67 21.763704217160093 27.655004811071876 28.042507666652156 22.538783305115878 68-69 21.85366423763143 27.50016870564142 28.141201242847124 22.504965813880023 70-71 21.74808982994902 27.49111847333097 28.35306055086248 22.40773114585753 72-73 21.851470924986838 27.630265570902086 27.932797977050004 22.585465527061068 74-75 21.755269297079032 27.66199721891927 27.948638764411847 22.634094719589847 76-77 21.781486831565903 27.53862411404395 28.171690874078102 22.508198180312043 78-79 21.90502992310726 27.616814055217343 27.880262582998512 22.597893438676884 80-81 21.651168795097536 27.67736815236292 28.0053368879987 22.66612616454084 82-83 21.774039357245513 27.718359461147152 27.97229452555512 22.535306656052217 84-85 21.753776867162344 27.614222878560795 27.830333762833035 22.80166649144383 86-87 21.717685902241207 27.38914129152235 28.032535734491663 22.860637071744776 88-89 21.79308850845153 27.35950939505439 27.955112640162977 22.89228945633111 90-91 21.694365009819034 27.62094329215338 28.01566057443296 22.66903112359463 92-93 21.696434662206254 27.486933076962384 28.030955227099298 22.785677033732064 94-95 21.542076915327808 27.39082829559225 28.053355228475947 23.013739560604 96-97 21.69593089740897 27.361128667829366 27.94600577664252 22.996934658119148 98-99 21.44505052530177 27.318161480478135 27.952044361532803 23.28474363268729 100-101 21.400818928410146 27.372585531612753 28.014202517910235 23.212393022066863 102-103 21.577380515775566 27.210760108322845 28.08085875878607 23.131000617115518 104-105 21.50447906578612 27.02038397301534 28.154948610363697 23.320188350834847 106-107 21.185380637980963 27.24140274834304 28.12349939902612 23.449717214649876 108-109 21.38314388892013 27.230411512873985 27.7933169194358 23.59312767877008 110-111 21.23051999412229 27.18737553566272 28.010744183351356 23.571360286863634 112-113 21.291516830691624 27.184337124342424 27.953573911963435 23.570572133002514 114-115 21.13569126801336 27.23091818621914 27.894924719194524 23.738465826572973 116-117 20.921558032581668 27.167918083730434 28.06456187362929 23.845962010058606 118-119 21.00560025134505 27.134678648751404 27.884924064699344 23.974797035204197 120-121 20.896261372157863 27.12569405340883 28.002874381742636 23.97517019269067 122-123 21.004097308509873 27.109253247308924 27.836612304835057 24.05003713934614 124-125 20.812115717247114 27.056904533809057 27.805309942241134 24.325669806702688 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 317.0 1 273.0 2 350.0 3 501.5 4 419.0 5 347.5 6 667.5 7 1648.0 8 2565.5 9 2889.0 10 2462.5 11 1766.0 12 1567.5 13 1623.0 14 1827.5 15 2030.0 16 2282.5 17 2573.5 18 2843.0 19 3272.5 20 3858.5 21 4585.0 22 5422.5 23 6609.0 24 8256.5 25 10381.5 26 13269.5 27 16570.5 28 19963.5 29 23417.5 30 27312.5 31 31891.0 32 36508.0 33 41117.5 34 45940.5 35 50292.0 36 54811.5 37 59542.0 38 62821.0 39 64946.5 40 66503.0 41 68869.0 42 71052.0 43 72714.5 44 74652.5 45 75408.5 46 74876.0 47 74196.0 48 75337.5 49 75568.5 50 73240.0 51 70171.5 52 66819.5 53 63760.5 54 59956.0 55 54694.5 56 49649.5 57 44864.0 58 40247.5 59 35760.5 60 30611.5 61 24759.5 62 19501.5 63 15089.0 64 11390.5 65 8788.5 66 6605.0 67 5042.0 68 3862.5 69 2808.5 70 2035.5 71 1555.0 72 1252.0 73 1034.0 74 840.0 75 683.5 76 471.0 77 262.0 78 157.5 79 107.0 80 69.5 81 42.0 82 33.5 83 25.5 84 17.0 85 14.5 86 11.0 87 6.5 88 3.5 89 2.5 90 2.0 91 3.0 92 2.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08899690222321108 2 2.933963809556409E-4 3 0.0 4 0.1742774502876507 5 0.0 6 9.779879365188031E-5 7 0.024889792984403535 8 0.0 9 0.0 10-11 4.1564487302049125E-4 12-13 3.9119517460752126E-4 14-15 2.4449698412970077E-4 16-17 0.0030317626032082897 18-19 0.0011002364285836535 20-21 0.002200472857167307 22-23 0.0010513370317577133 24-25 0.003105111698447199 26-27 0.0027139165238396785 28-29 0.001980425571450576 30-31 6.60141857150192E-4 32-33 4.889939682594016E-5 34-35 9.29088539692863E-4 36-37 0.0010024376349317733 38-39 4.889939682594015E-4 40-41 0.0011002364285836535 42-43 5.378933650853416E-4 44-45 0.0015403310000171148 46-47 9.779879365188031E-5 48-49 2.444969841297008E-5 50-51 0.030146478143192105 52-53 7.334909523891023E-5 54-55 0.12831201727126698 56-57 2.4449698412970077E-4 58-59 0.0 60-61 0.0 62-63 3.4229577778158106E-4 64-65 0.04048870057187845 66-67 0.0377503343496258 68-69 0.001613680095256025 70-71 0.001955975873037606 72-73 0.06195553577846617 74-75 0.006185773698481429 76-77 0.0023960704444710673 78-79 0.0011491358254095935 80-81 1.4669819047782046E-4 82-83 1.9559758730376063E-4 84-85 0.0 86-87 9.779879365188031E-5 88-89 0.0014425322063652344 90-91 3.4229577778158106E-4 92-93 0.0030073129047953192 94-95 0.005721229428634998 96-97 0.0027139165238396785 98-99 0.0 100-101 0.0012469346190614738 102-103 8.312897460409825E-4 104-105 7.334909523891023E-5 106-107 4.889939682594016E-5 108-109 0.0 110-111 7.334909523891023E-5 112-113 4.889939682594016E-5 114-115 2.4449698412970077E-4 116-117 0.0 118-119 8.557394444539527E-4 120-121 0.002616117730187798 122-123 6.845915555631621E-4 124-125 0.006552519174675981 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2045015.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.65706586108817 #Duplication Level Percentage of deduplicated Percentage of total 1 82.0667215242046 53.882601401182825 2 10.321193199033882 13.553185232679658 3 3.385743745345589 6.668940002307679 4 1.484904816682832 3.8997797338556706 5 0.806041552446477 2.646116164787605 6 0.4658647095891837 1.835238595191225 7 0.29589022373400065 1.3599098735148776 8 0.20687025683947263 1.0865995262407582 9 0.1476043654144379 0.8722142587259886 >10 0.7500018668803641 8.7554617165901 >50 0.04829451805323196 2.1539285518275006 >100 0.020113695295517615 2.190682544003943 >500 3.7776324014986466E-4 0.13606830309815854 >1k 2.2665794408989064E-4 0.35299381875551733 >5k 1.5110529605992713E-4 0.6062802772386078 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6139 0.3001933971144466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6083 0.29745503089219394 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4308 0.21065860152615018 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.889939682594015E-5 0.0 0.0 0.0 0.0 7 9.77987936518803E-5 0.0 0.0 0.0 4.889939682594015E-5 8 9.77987936518803E-5 0.0 0.0 0.0 4.889939682594015E-5 9 9.77987936518803E-5 0.0 0.0 0.0 4.889939682594015E-5 10-11 9.77987936518803E-5 0.0 0.0 9.77987936518803E-5 4.889939682594015E-5 12-13 1.4669819047782046E-4 0.0 0.0 9.77987936518803E-5 4.889939682594015E-5 14-15 1.4669819047782046E-4 0.0 0.0 1.4669819047782046E-4 4.889939682594015E-5 16-17 1.4669819047782046E-4 0.0 0.0 2.933963809556409E-4 4.889939682594015E-5 18-19 1.7114788889079053E-4 0.0 0.0 3.1784607936861096E-4 4.889939682594015E-5 20-21 1.955975873037606E-4 0.0 0.0 6.60141857150192E-4 4.889939682594015E-5 22-23 1.955975873037606E-4 0.0 0.0 9.290885396928629E-4 4.889939682594015E-5 24-25 2.4449698412970077E-4 0.0 0.0 0.0012224849206485039 4.889939682594015E-5 26-27 2.4449698412970077E-4 0.0 0.0 0.001662579492081965 4.889939682594015E-5 28-29 3.6674547619455116E-4 0.0 0.0 0.002273821952406217 4.889939682594015E-5 30-31 3.911951746075212E-4 0.0 0.0 0.004107549333378973 4.889939682594015E-5 32-33 3.911951746075212E-4 0.0 0.0 0.008410696254061706 4.889939682594015E-5 34-35 4.1564487302049125E-4 0.0 0.0 0.013325085635068692 4.889939682594015E-5 36-37 4.4009457143346135E-4 0.0 0.0 0.02261597103199732 4.889939682594015E-5 38-39 4.889939682594015E-4 0.0 0.0 0.03977965931790231 4.889939682594015E-5 40-41 5.134436666723715E-4 0.0 0.0 0.062248932159421816 4.889939682594015E-5 42-43 5.623430634983117E-4 0.0 0.0 0.08474265469935428 4.889939682594015E-5 44-45 6.35692158737222E-4 0.0 0.0 0.11092828169964523 4.889939682594015E-5 46-47 6.601418571501921E-4 0.0 0.0 0.14606249831908324 4.889939682594015E-5 48-49 7.334909523891023E-4 0.0 0.0 0.1862089031131801 4.889939682594015E-5 50-51 7.334909523891023E-4 0.0 0.0 0.2335190695422772 4.889939682594015E-5 52-53 7.334909523891023E-4 0.0 0.0 0.27669723693958237 4.889939682594015E-5 54-55 7.334909523891023E-4 0.0 0.0 0.32351840940042004 4.889939682594015E-5 56-57 8.312897460409826E-4 0.0 0.0 0.3800216624327939 4.889939682594015E-5 58-59 8.801891428669227E-4 0.0 0.0 0.448603066481175 4.889939682594015E-5 60-61 9.290885396928629E-4 0.0 0.0 0.5249105752280545 4.889939682594015E-5 62-63 9.290885396928629E-4 0.0 0.0 0.6063280709432449 4.889939682594015E-5 64-65 0.0010268873333447433 0.0 0.0 0.6859362889758754 4.889939682594015E-5 66-67 0.0010268873333447433 0.0 0.0 0.7809233673102642 4.889939682594015E-5 68-69 0.0011246861269966235 0.0 0.0 0.8938809739781859 4.889939682594015E-5 70-71 0.0012224849206485039 0.0 0.0 1.0218995948684972 4.889939682594015E-5 72-73 0.0012224849206485039 0.0 0.0 1.1580355156319146 4.889939682594015E-5 74-75 0.0012224849206485039 0.0 0.0 1.2945137321731135 4.889939682594015E-5 76-77 0.0012224849206485039 0.0 0.0 1.4447815786192277 4.889939682594015E-5 78-79 0.0012469346190614738 0.0 0.0 1.6195235731767248 4.889939682594015E-5 80-81 0.0013691831111263242 0.0 0.0 1.8261968738615608 4.889939682594015E-5 82-83 0.0013691831111263242 0.0 0.0 2.0452417219433596 4.889939682594015E-5 84-85 0.001613680095256025 0.0 0.0 2.257636252056831 4.889939682594015E-5 86-87 0.0018337273809727558 0.0 0.0 2.4785637269164287 4.889939682594015E-5 88-89 0.0018581770793857258 0.0 0.0 2.7389774647129728 4.889939682594015E-5 90-91 0.0021026740635154265 0.0 0.0 3.037850578113119 4.889939682594015E-5 92-93 0.0021026740635154265 0.0 0.0 3.366405625386611 4.889939682594015E-5 94-95 0.0021026740635154265 0.0 0.0 3.686354378818737 4.889939682594015E-5 96-97 0.0021026740635154265 0.0 0.0 4.007207771092144 4.889939682594015E-5 98-99 0.002273821952406217 0.0 0.0 4.360628161651626 4.889939682594015E-5 100-101 0.0023716207460580977 0.0 0.0 4.776639780148312 4.889939682594015E-5 102-103 0.0024694195397099777 0.0 0.0 5.230059437216842 4.889939682594015E-5 104-105 0.002591668031774828 0.0 0.0 5.6847015792060205 4.889939682594015E-5 106-107 0.0027628159206656185 0.0 0.0 6.1329134505125875 4.889939682594015E-5 108-109 0.002860614714317499 0.0 0.0 6.598435708295538 4.889939682594015E-5 110-111 0.0029828632063823492 0.0 0.0 7.123297384126767 4.889939682594015E-5 112-113 0.0030317626032082892 0.0 0.0 7.6941000432759665 4.889939682594015E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4630 0.0 64.665726 1 GTATCAA 8235 0.0 50.48014 1 ATCAACG 10480 0.0 37.635796 3 TATCAAC 11020 0.0 37.467613 2 TCAACGC 10620 0.0 36.859142 4 CAACGCA 10760 0.0 36.27089 5 AACGCAG 11085 0.0 34.992786 6 GTACATG 9075 0.0 29.443048 1 ACGCAGA 13130 0.0 29.362127 7 CGCAGAG 13230 0.0 29.086685 8 TACATGG 9295 0.0 27.69101 2 GCAGAGT 13965 0.0 26.278103 9 GTGGTAT 2425 0.0 25.824324 1 TGGTATC 2345 0.0 25.407476 2 ACATGGG 9455 0.0 25.331968 3 GAGTACT 9280 0.0 24.355444 12-13 AGTACTT 9060 0.0 24.09341 12-13 CAGAGTA 13845 0.0 23.950375 10-11 GTACTTT 9615 0.0 23.754307 14-15 AGAGTAC 13310 0.0 23.594799 10-11 >>END_MODULE