FastQCFastQC Report
Thu 26 May 2016
SRR1364485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872682
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28500.15218814513088713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20430.10909487035172015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67400.049.640041
GGTATCA38700.044.3034171
ATCAACG76450.041.484413
TCAACGC77100.040.182924
CAACGCA78450.039.795225
TATCAAC85300.039.133532
AACGCAG80250.037.990376
GTACATG97300.034.9976541
TACATGG98250.033.067042
ACGCAGA93850.031.7254337
CGCAGAG93050.031.3615868
ACATGGG100350.030.0032543
GCAGAGT96800.027.931799
CATGGGA69600.025.9373684
GAGTACT40600.025.27414312-13
CGCATAG2251.896151E-723.8220028
ACGCATA2302.3467692E-723.3016417
GTACTTT45850.022.67840614-15
CAGAGTA98250.022.263510-11
CATGGGG35200.022.0035744
Produced by FastQC (version 0.11.5)