##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364485_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1872682 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.680477518340005 30.0 23.0 31.0 2.0 34.0 2 24.953748153717502 30.0 24.0 31.0 2.0 34.0 3 24.94042021015848 30.0 23.0 31.0 2.0 34.0 4 27.575648721993375 33.0 24.0 35.0 2.0 37.0 5 28.12983037162743 35.0 26.0 35.0 2.0 37.0 6 28.27007628631022 35.0 27.0 36.0 2.0 37.0 7 28.050277623216328 35.0 26.0 37.0 2.0 37.0 8 27.70337943121149 35.0 25.0 37.0 2.0 37.0 9 28.163595314100313 34.0 23.0 39.0 2.0 39.0 10-11 28.23212803882346 34.5 24.0 39.0 2.0 39.0 12-13 28.05275855697871 34.5 22.5 39.0 2.0 39.0 14-15 28.59279365103098 34.5 22.5 39.0 2.0 41.0 16-17 28.835418399920542 35.0 24.0 39.5 2.0 41.0 18-19 28.801924726141436 35.0 23.5 40.0 2.0 41.0 20-21 28.587714838931543 34.5 21.5 40.0 2.0 41.0 22-23 28.62587454784101 34.5 22.5 40.0 2.0 41.0 24-25 28.64541069973439 35.0 23.0 40.0 2.0 41.0 26-27 28.731807375731705 35.0 23.5 40.0 2.0 41.0 28-29 28.656959910972606 35.0 24.0 40.0 2.0 41.0 30-31 28.296638724567224 34.0 22.0 39.0 2.0 41.0 32-33 28.281995555038172 34.5 23.0 39.0 2.0 41.0 34-35 27.991249982645215 34.0 21.0 39.0 2.0 41.0 36-37 27.754304788533236 34.0 19.0 39.0 2.0 41.0 38-39 27.601942561524062 34.0 18.0 39.0 2.0 41.0 40-41 27.65337120771172 34.0 18.5 39.0 2.0 41.0 42-43 27.49907245330494 34.0 18.0 39.0 2.0 41.0 44-45 27.161291399180428 33.0 15.5 39.0 2.0 40.5 46-47 26.884916392639006 33.0 14.5 39.0 2.0 40.0 48-49 26.625853188101345 33.0 12.5 39.0 2.0 40.0 50-51 26.613917365575148 33.0 12.5 39.0 2.0 40.0 52-53 26.415595653720175 33.0 9.0 39.0 2.0 40.0 54-55 26.300013029441196 33.0 8.5 38.5 2.0 40.0 56-57 25.911848888385748 33.0 8.0 38.0 2.0 40.0 58-59 25.70073936738859 32.0 7.0 38.0 2.0 40.0 60-61 25.31284142208875 32.0 6.5 38.0 2.0 40.0 62-63 25.250553484254134 32.0 2.0 38.0 2.0 40.0 64-65 25.078210288773 32.0 2.0 37.5 2.0 40.0 66-67 24.86644876172249 31.5 2.0 37.0 2.0 40.0 68-69 24.703098817631613 31.5 2.0 36.5 2.0 40.0 70-71 24.588849041107885 31.0 2.0 36.0 2.0 39.0 72-73 24.5430655071176 31.5 2.0 36.0 2.0 39.0 74-75 24.263546613893872 31.0 2.0 36.0 2.0 39.0 76-77 24.015651349241356 31.0 2.0 35.0 2.0 38.5 78-79 23.710391566747585 31.0 2.0 35.0 2.0 37.0 80-81 23.68910338220798 31.0 2.0 35.0 2.0 37.0 82-83 23.483159981246146 31.0 2.0 35.0 2.0 36.5 84-85 23.277182137704106 31.0 2.0 35.0 2.0 36.0 86-87 23.07493530668848 31.0 2.0 35.0 2.0 36.0 88-89 22.850753625014818 31.0 2.0 35.0 2.0 35.5 90-91 22.674494655259142 30.5 2.0 35.0 2.0 35.0 92-93 22.56053296822418 30.0 2.0 34.5 2.0 35.0 94-95 22.47307711613611 30.0 2.0 34.0 2.0 35.0 96-97 22.344221282631008 30.0 2.0 34.0 2.0 35.0 98-99 22.23452807257185 30.0 2.0 34.0 2.0 35.0 100-101 22.173169283412772 30.0 2.0 34.0 2.0 35.0 102-103 21.144722649120354 28.5 2.0 33.5 2.0 34.5 104-105 21.828725325495732 30.0 2.0 34.0 2.0 35.0 106-107 21.91448307828024 30.0 2.0 34.0 2.0 35.0 108-109 21.84722019008032 30.0 2.0 34.0 2.0 35.0 110-111 21.72657610849039 30.0 2.0 34.0 2.0 35.0 112-113 21.693265060485444 30.0 2.0 34.0 2.0 35.0 114-115 21.630234871697382 30.0 2.0 34.0 2.0 35.0 116-117 21.453646427957338 30.0 2.0 34.0 2.0 35.0 118-119 21.28815970890947 30.0 2.0 34.0 2.0 35.0 120-121 21.13100836127009 30.0 2.0 34.0 2.0 35.0 122-123 20.8607534541369 30.0 2.0 34.0 2.0 35.0 124-125 19.94467079835231 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6480835866759804 1101 2 1.3476786453588652 1101 3 1.673903154289416 1101 4 1.9714223727851277 1101 5 1.4433000564948593 1101 6 1.3544220327834076 1101 7 1.4239260947281025 1101 8 1.759674914999085 1101 9 2.12417730901754 1101 10-11 2.0034719483644956 1101 12-13 1.8681290107806454 1101 14-15 2.167880376391267 1101 16-17 1.8797761129212596 1101 18-19 1.77903303702303 1101 20-21 2.2083692212967634 1101 22-23 2.136996645918291 1101 24-25 1.8474291779551884 1101 26-27 1.4915988298241878 1101 28-29 1.531208534717777 1101 30-31 1.6162584463274605 1101 32-33 2.010678965578226 1101 34-35 2.1976077009845376 1101 36-37 2.1115941336912876 1101 38-39 2.0126810216858786 1101 40-41 1.8663911352643083 1101 42-43 1.8937338708614142 1101 44-45 1.9800406910869306 1101 46-47 2.0143626130145016 1101 48-49 2.223258437527786 1101 50-51 2.083248003041515 1101 52-53 1.988424962105146 1101 54-55 1.9626889674377104 1101 56-57 2.2568812305015733 1101 58-59 2.424180630848337 1101 60-61 2.592240842708229 1101 62-63 2.2146391322540175 1101 64-65 2.2108919688763002 1101 66-67 2.2472503261673182 1101 68-69 2.08135550457704 1101 70-71 2.2114170957159445 1101 72-73 1.9922613746906634 1101 74-75 2.062200340672156 1101 76-77 2.2309923545225487 1101 78-79 2.0924347831644425 1101 80-81 2.0408308795810086 1101 82-83 2.06027924253819 1101 84-85 1.9172615234055534 1101 86-87 1.922772828477978 1101 88-89 2.006689073537256 1101 90-91 2.019630132553999 1101 92-93 1.987533028909322 1101 94-95 1.8867554843572982 1101 96-97 2.0118690068375997 1101 98-99 1.955144269304114 1101 100-101 1.8824121628246857 1101 102-103 1.9096532245492774 1101 104-105 1.936170381203592 1101 106-107 2.08567704359767 1101 108-109 2.0618047062892018 1101 110-111 2.217834717926369 1101 112-113 2.2403658169972758 1101 114-115 2.121747568578172 1101 116-117 2.2157833164437903 1101 118-119 2.1391112876125966 1101 120-121 2.0962381464416318 1101 122-123 2.2151494377731566 1101 124-125 2.1271402789756344 1102 1 0.7437539242206412 1102 2 0.5336870049694511 1102 3 0.6205268910652713 1102 4 1.1535380552953534 1102 5 1.0668985219922504 1102 6 1.1787163672781595 1102 7 1.1255023645536824 1102 8 1.0638217930624592 1102 9 1.4031824246734388 1102 10-11 1.2161390871822952 1102 12-13 1.4704257393808895 1102 14-15 1.7563146608681492 1102 16-17 1.3124794431400417 1102 18-19 1.5965844274304573 1102 20-21 1.4545409881436449 1102 22-23 1.4778700067378097 1102 24-25 1.3016443374786526 1102 26-27 1.3367809409382296 1102 28-29 0.9906064495092295 1102 30-31 1.0061331344010505 1102 32-33 0.8093136447478742 1102 34-35 1.2970758628193018 1102 36-37 1.551968149571966 1102 38-39 1.5182858462618611 1102 40-41 1.1991771661693065 1102 42-43 1.244955838886483 1102 44-45 1.5301619210874655 1102 46-47 1.4940022659891277 1102 48-49 1.603978087683668 1102 50-51 1.5194945796888106 1102 52-53 1.5527119824831424 1102 54-55 1.2858499222063386 1102 56-57 1.4596535822722139 1102 58-59 1.4711516739585662 1102 60-61 1.8917295969110732 1102 62-63 1.5999704792176068 1102 64-65 1.8131640848569006 1102 66-67 1.6033104156795908 1102 68-69 1.5257915734974006 1102 70-71 1.7596827854614823 1102 72-73 1.6255862220952757 1102 74-75 1.7167523447390067 1102 76-77 1.5278525837385644 1102 78-79 1.4058091664641381 1102 80-81 1.0997132313733502 1102 82-83 1.0293028616303168 1102 84-85 1.057038132188591 1102 86-87 1.179221420481948 1102 88-89 0.9411543475293946 1102 90-91 0.9414240761592367 1102 92-93 0.8097001135336761 1102 94-95 1.0665868188147023 1102 96-97 1.1602795626933435 1102 98-99 1.3244327567312908 1102 100-101 1.1364646506351583 1102 102-103 0.9448867891727097 1102 104-105 0.9441161457258112 1102 106-107 1.2540789856075953 1102 108-109 1.2864207865416475 1102 110-111 1.1356163476838113 1102 112-113 0.9990945312214627 1102 114-115 1.0836896630218753 1102 116-117 1.3938066227100947 1102 118-119 1.5175953581081636 1102 120-121 1.393537862248678 1102 122-123 1.4933063031921563 1102 124-125 1.3565730089171772 1103 1 1.2421040848082434 1103 2 1.012051469242433 1103 3 1.1953723441064668 1103 4 1.7620774800878074 1103 5 1.3062664735674225 1103 6 1.2198938112109659 1103 7 1.3639183525568654 1103 8 1.5042042954873693 1103 9 2.2244843231311187 1103 10-11 1.8348982736307669 1103 12-13 1.9809252052427802 1103 14-15 2.0698883459641415 1103 16-17 2.0233331433012864 1103 18-19 1.6460695709138697 1103 20-21 2.1269327637322526 1103 22-23 1.8856311407306627 1103 24-25 1.9522499940115807 1103 26-27 1.6215963257772934 1103 28-29 1.5640838443553307 1103 30-31 1.8455936514522762 1103 32-33 1.791400805840187 1103 34-35 1.546173055184486 1103 36-37 1.9934268900860808 1103 38-39 2.0290312648938986 1103 40-41 2.0767627857729707 1103 42-43 2.1245336586198924 1103 44-45 2.006493164254959 1103 46-47 2.113940156424004 1103 48-49 1.9065867101889147 1103 50-51 1.7888933471902284 1103 52-53 1.8655851954744307 1103 54-55 1.7661423723390257 1103 56-57 1.9952840210131875 1103 58-59 2.1146977417259087 1103 60-61 2.1546986293629935 1103 62-63 1.9372819817761346 1103 64-65 1.6981563272192126 1103 66-67 1.9040388235880208 1103 68-69 1.8091997830927795 1103 70-71 1.8337614751165106 1103 72-73 1.7447782923425699 1103 74-75 1.7414312214635324 1103 76-77 1.810099917028971 1103 78-79 1.9380859240831327 1103 80-81 1.4565254480776488 1103 82-83 1.5970812832816428 1103 84-85 1.5467088081796554 1103 86-87 1.5677665639792053 1103 88-89 1.642671362060856 1103 90-91 1.6876684933255959 1103 92-93 1.674308911892556 1103 94-95 1.4761914735357813 1103 96-97 1.587825095976413 1103 98-99 1.5275490700636993 1103 100-101 1.3516996333232676 1103 102-103 1.3599548493755798 1103 104-105 1.5251647664863732 1103 106-107 1.394750642640389 1103 108-109 1.6478903552086805 1103 110-111 1.8008891665968 1103 112-113 1.6896163664964554 1103 114-115 1.5852818653670973 1103 116-117 1.6816696756538114 1103 118-119 1.735017433831846 1103 120-121 1.7172430360149171 1103 122-123 1.6928859853124791 1103 124-125 1.6319587618649756 1104 1 1.1876953497308023 1104 2 1.0921789879477295 1104 3 1.08906509506323 1104 4 1.4891027955842517 1104 5 1.1443258011541317 1104 6 1.1285395987598932 1104 7 1.1055087933326249 1104 8 1.4223916909177028 1104 9 1.838915304900386 1104 10-11 1.6719257153908025 1104 12-13 1.224927516456166 1104 14-15 1.5984812097578462 1104 16-17 1.102267933564491 1104 18-19 0.6243508206736195 1104 20-21 -3.765921068605273 1104 22-23 -0.7491345979581325 1104 24-25 0.7799602058098394 1104 26-27 1.114615899044093 1104 28-29 0.6399486218982986 1104 30-31 1.3135827310678891 1104 32-33 1.1131166785956133 1104 34-35 1.3796362597317042 1104 36-37 1.5912192002604932 1104 38-39 1.4846795589297983 1104 40-41 1.5472132681031283 1104 42-43 1.4516021473714495 1104 44-45 1.5609747699881389 1104 46-47 1.5608449053063431 1104 48-49 1.5760063720207498 1104 50-51 1.4707373185410226 1104 52-53 1.309993341875586 1104 54-55 1.3454400881212827 1104 56-57 1.6209650898208778 1104 58-59 1.6356158482881789 1104 60-61 1.7231472938256367 1104 62-63 1.4318045466387055 1104 64-65 1.655877893001481 1104 66-67 1.5198086045938553 1104 68-69 1.392686820243302 1104 70-71 1.5927272016364 1104 72-73 1.5408351277199088 1104 74-75 1.5077710519818766 1104 76-77 1.4756635295420324 1104 78-79 1.4207366674956141 1104 80-81 1.1618451926536437 1104 82-83 1.1474422099371857 1104 84-85 1.1521356786601125 1104 86-87 1.043048969345648 1104 88-89 1.1988327819304807 1104 90-91 1.2045680605386977 1104 92-93 0.9337101600180766 1104 94-95 1.1042046229824614 1104 96-97 1.1384847538808103 1104 98-99 1.0567235262575068 1104 100-101 0.6682024810447942 1104 102-103 0.9993331303397852 1104 104-105 1.1761584213028513 1104 106-107 1.3015856465623372 1104 108-109 1.3569163441683685 1104 110-111 1.3572376229799517 1104 112-113 1.3937388796960946 1104 114-115 1.310467073249999 1104 116-117 1.4519161311306625 1104 118-119 1.4786287585577647 1104 120-121 1.4290896739814194 1104 122-123 1.236652419649701 1104 124-125 1.5189322562759564 1105 1 1.2436626877449726 1105 2 0.9251401759644615 1105 3 0.9808737566112242 1105 4 1.5094583867237326 1105 5 1.1240451319369562 1105 6 1.0121800053111798 1105 7 0.9679656056442738 1105 8 1.3452201247913287 1105 9 1.6164635109230936 1105 10-11 1.346995958830778 1105 12-13 1.534987312756808 1105 14-15 1.8308377039326942 1105 16-17 1.5243385967437746 1105 18-19 1.1375323894835034 1105 20-21 1.3266371873271297 1105 22-23 1.280290462906276 1105 24-25 1.2767257083582884 1105 26-27 1.214227573976082 1105 28-29 0.8399408869546257 1105 30-31 1.222700066963501 1105 32-33 1.1105608369366387 1105 34-35 1.0459283351770843 1105 36-37 1.2956388654223936 1105 38-39 1.1688792427350094 1105 40-41 1.313707345850677 1105 42-43 1.567248051580851 1105 44-45 1.7464686500036066 1105 46-47 2.161996672625115 1105 48-49 1.525702756171814 1105 50-51 1.6548652803292967 1105 52-53 1.684964818581161 1105 54-55 1.7923761125235416 1105 56-57 1.912314487709029 1105 58-59 1.5643743688637919 1105 60-61 2.072738996934447 1105 62-63 1.7400498862523897 1105 64-65 1.472080404198941 1105 66-67 1.6625058467609222 1105 68-69 1.205541458031668 1105 70-71 1.3330319848990833 1105 72-73 1.2870201232974203 1105 74-75 1.6684844234374836 1105 76-77 1.5071414400435472 1105 78-79 1.3596552186490811 1105 80-81 1.4298414052897854 1105 82-83 1.4435616736595271 1105 84-85 1.4511026719672309 1105 86-87 1.3904048213325915 1105 88-89 1.5218189270022755 1105 90-91 1.5157223899885963 1105 92-93 1.4250402922690597 1105 94-95 1.3672411478057818 1105 96-97 1.5454109269881862 1105 98-99 1.3135483727061867 1105 100-101 1.4569713759343017 1105 102-103 1.4017914079874068 1105 104-105 1.491821693274506 1105 106-107 1.5598758289878383 1105 108-109 1.5577811351461364 1105 110-111 1.4282212893388397 1105 112-113 1.4552519847430325 1105 114-115 1.3762141496399103 1105 116-117 1.4682517032207762 1105 118-119 1.447764733350457 1105 120-121 1.4902817402463846 1105 122-123 1.6148596627348155 1105 124-125 1.2050231944970413 1106 1 -0.9372954079422513 1106 2 -0.8262427686821638 1106 3 -0.8172442419117338 1106 4 -1.1266754632767118 1106 5 -1.6523312505627992 1106 6 -1.2495019507869465 1106 7 -1.1254729148251776 1106 8 -0.918369525257166 1106 9 -0.8030221724744528 1106 10-11 -3.0991949929696965 1106 12-13 -1.3931120225755684 1106 14-15 -1.0585223866471765 1106 16-17 -1.2707360671408345 1106 18-19 -3.9759186213095603 1106 20-21 -6.228569598155175 1106 22-23 -7.070113554017389 1106 24-25 -6.931923256824515 1106 26-27 -5.96631304682526 1106 28-29 -2.828693330081151 1106 30-31 -4.544047366013626 1106 32-33 -4.72321942679493 1106 34-35 -4.1940868022498385 1106 36-37 -6.5587428691198575 1106 38-39 -7.123846673073757 1106 40-41 -3.420350641283065 1106 42-43 -2.133676432567672 1106 44-45 -1.3335708563449913 1106 46-47 -1.0824162710427494 1106 48-49 -0.9403375776746259 1106 50-51 -1.0985088866117216 1106 52-53 -1.1446457569302453 1106 54-55 -1.0909174957229624 1106 56-57 -0.9570155949517805 1106 58-59 -0.8967015340431281 1106 60-61 -0.7370228638679599 1106 62-63 -1.039711563025552 1106 64-65 -1.1021032202416094 1106 66-67 -1.3416627848423737 1106 68-69 -1.2179489145203135 1106 70-71 -0.9318596951884679 1106 72-73 -0.9831064816479902 1106 74-75 -1.055092591697722 1106 76-77 -1.07402063831071 1106 78-79 -0.9537530269665133 1106 80-81 -1.3241576671118302 1106 82-83 -1.4486836483083145 1106 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-4.71776566225741 2216 84-85 -4.512319061076592 2216 86-87 -4.771007908864338 2216 88-89 -4.92866909132945 2216 90-91 -4.978254949961723 2216 92-93 -4.76385080471027 2216 94-95 -7.746975224704023 2216 96-97 -9.398123497095142 2216 98-99 -6.617574050550299 2216 100-101 -5.617687488096998 2216 102-103 -6.6241251782916954 2216 104-105 -8.931776159957412 2216 106-107 -5.992620330895463 2216 108-109 -7.714711907268249 2216 110-111 -5.6523315656240705 2216 112-113 -5.26545662182399 2216 114-115 -5.683722656492179 2216 116-117 -5.307146950469598 2216 118-119 -5.621564813364522 2216 120-121 -6.406690638642889 2216 122-123 -8.98938847686729 2216 124-125 -7.683277516050593 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 248017.0 3 34354.0 4 19161.0 5 15621.0 6 15126.0 7 16613.0 8 18058.0 9 19254.0 10 20044.0 11 20847.0 12 20952.0 13 21940.0 14 22386.0 15 18884.0 16 15318.0 17 13616.0 18 12092.0 19 11646.0 20 11433.0 21 12525.0 22 15921.0 23 21338.0 24 28262.0 25 35621.0 26 41841.0 27 46587.0 28 51892.0 29 58510.0 30 64586.0 31 71193.0 32 82490.0 33 97713.0 34 118079.0 35 142804.0 36 177766.0 37 180070.0 38 49971.0 39 151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70533151300618 19.478587457489766 14.273763234166841 22.542317795337212 2 14.66170655005692 20.926560324017267 41.16220147553498 23.24953165039084 3 18.929377189416112 27.028206704922404 28.75719432051369 25.285221785147794 4 10.946694663054918 17.248339718005845 38.62935239652289 33.175613222416345 5 12.926902376745373 39.49245375463576 33.548266102726394 14.032377765892479 6 29.88242606972411 37.8514147908079 18.379757867232293 13.886401272235688 7 27.52112260885446 33.7645610096559 21.09217051026413 17.622145871225513 8 25.06959489443417 36.70899054427934 20.04461122179958 18.176803339486913 9 26.078881007742417 17.47031575259727 20.5304874336597 35.920315806000616 10-11 23.847362728732012 28.501488721836655 27.24625468364928 20.404893865782054 12-13 24.905682847108352 25.93401951415151 27.818813872184737 21.3414837665554 14-15 22.180906487107443 26.951080330072518 26.099436189308374 24.768576993511665 16-17 21.400333453422142 30.45920483403308 26.222482281114107 21.91797943143067 18-19 21.223206407602298 28.941619718460316 28.833222238601575 21.001951635335818 20-21 22.48724831173984 27.909172312025397 28.852865423399898 20.75071395283486 22-23 21.968376301186225 28.10297864158562 28.32876683063349 21.599878226594672 24-25 21.532997390972284 28.06015985301854 28.792556927275687 21.614285828733486 26-27 21.561216890655484 28.319812830419224 28.367796129988015 21.751174148937277 28-29 21.405059127106192 28.270334555179605 28.559668606240812 21.764937711473394 30-31 21.576887791447145 28.034489314005164 28.802256495916456 21.586366398631235 32-33 21.54747396854116 28.174401428646195 28.362648207623646 21.915476395189 34-35 21.764613359603302 27.93321346636733 28.70237911804582 21.59979405598355 36-37 21.726126108806852 27.795907447369224 28.62896443006887 21.849002013755054 38-39 21.600745320120225 28.052229017711234 28.66144650635199 21.68557915581655 40-41 21.814785455135958 27.989387069617578 28.546768900075637 21.64905857517083 42-43 21.57199374588589 28.027296991642935 28.404172791933206 21.99653647053797 44-45 21.682626959507413 28.12778748240804 28.353926900299513 21.835658657785036 46-47 21.714228955829793 28.274503270057572 27.954439743308356 22.05682803080428 48-49 21.722219373913195 28.176523240065453 27.966371439435694 22.134885946585662 50-51 21.87464618132179 28.336950739894938 27.693828547746563 22.094574531036706 52-53 21.946459752686987 28.426176074667627 27.625814827939156 22.00154934470623 54-55 21.808341046040724 28.352250606290895 27.702727601416537 22.136680746251837 56-57 21.795431503120223 28.522705638299605 27.5521797514234 22.12968310715677 58-59 21.87308898308932 28.169691091543488 27.687039987352907 22.27017993801429 60-61 22.019759034857415 28.113890884616104 27.92081429231513 21.94553578821135 62-63 21.847271554322987 28.23136608397378 27.7995666785758 22.121795683127434 64-65 21.658235571345223 28.067229807760345 27.886378474071876 22.388156146822553 66-67 21.77205928493324 28.39366990078679 27.46533011656985 22.368940697710126 68-69 21.750351201883472 28.295670196798557 27.633987423754945 22.319991177563026 70-71 21.581280050283283 28.0008362746687 28.06521233553198 22.352671339516032 72-73 21.804359510205913 28.119238657004498 27.682908497491145 22.393493335298448 74-75 21.62700780734982 28.303452017546178 27.689004695730695 22.380535479373307 76-77 21.75850232509703 28.084982530063378 27.78698559815007 22.369529546689524 78-79 21.79887179944597 28.126553091735794 27.69208131183826 22.382493796979983 80-81 21.707664247316778 28.416961242073562 27.47896586075962 22.396408649850034 82-83 21.802641802641805 28.427815025753173 27.432399597348052 22.337143574256977 84-85 21.841983143430365 28.236596474570696 27.354945442836065 22.566474939162877 86-87 21.724796706179518 28.173059079700792 27.536883927272143 22.565260286847547 88-89 21.923028552218724 28.03284716067889 27.561912182388575 22.48221210471381 90-91 21.75592779362344 28.260306518770655 27.657794579341733 22.325971108264174 92-93 21.78482553855027 28.171091839241274 27.541412566350886 22.502670055857568 94-95 21.701981315757713 28.056001461074853 27.518645258929592 22.723371964237842 96-97 21.756466987478408 28.071036402778848 27.464167670816497 22.708328938926243 98-99 21.51382093198673 27.979787596205835 27.514475098291825 22.99191637351561 100-101 21.544162331372082 28.066382073401137 27.544998595546115 22.84445699968066 102-103 21.62754466556396 27.821783632171755 27.7442471768213 22.806424525442985 104-105 21.51144690038412 27.81326314226774 27.780714865884548 22.894575091463594 106-107 21.37458020921054 27.936986926076774 27.819373415021104 22.86905944969159 108-109 21.422300708782053 27.96858888131198 27.458349108669054 23.150761301236912 110-111 21.228685272154863 28.054391188386106 27.680396364557776 23.036527174901256 112-113 21.336162931902727 28.039700179324207 27.693975069769827 22.93016181900324 114-115 21.28477007918839 28.094576431027495 27.48591147283783 23.134742016946284 116-117 21.103460908312204 28.00514479550052 27.767663940291747 23.12373035589553 118-119 21.18802099743139 27.991840265725376 27.515259450713174 23.304879286130053 120-121 21.07604758888738 28.110964904317026 27.611001752701412 23.201985754094174 122-123 21.18982265204877 27.97622544176782 27.593066287868673 23.240885618314742 124-125 21.086029060153287 27.96162179057348 27.411247385307497 23.54110176396573 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1564.0 1 1685.0 2 1928.0 3 1751.0 4 1205.5 5 885.5 6 826.5 7 941.0 8 1195.5 9 1374.5 10 1280.0 11 1188.0 12 1289.0 13 1412.5 14 1538.5 15 1712.0 16 1882.5 17 2074.5 18 2387.5 19 2703.0 20 3159.0 21 3811.0 22 4607.5 23 5768.0 24 7225.5 25 9096.0 26 11881.5 27 14988.5 28 17961.0 29 21478.0 30 25728.5 31 30050.0 32 34559.0 33 39204.5 34 44080.5 35 48650.5 36 53611.5 37 58089.5 38 59653.5 39 60197.5 40 60472.0 41 61723.0 42 62983.5 43 64493.5 44 66434.0 45 67114.0 46 67048.5 47 66228.0 48 66633.5 49 66914.0 50 65139.0 51 62328.5 52 59795.0 53 57634.0 54 54165.0 55 49785.0 56 45370.5 57 41359.0 58 38079.5 59 34156.0 60 29274.5 61 23883.5 62 18690.5 63 14303.0 64 10777.5 65 8235.0 66 6236.5 67 4681.5 68 3549.0 69 2624.0 70 1968.0 71 1591.5 72 1315.5 73 1080.0 74 810.0 75 584.0 76 427.0 77 288.5 78 190.0 79 131.5 80 89.0 81 57.5 82 45.5 83 33.0 84 24.0 85 16.5 86 9.0 87 5.5 88 6.0 89 10.5 90 7.5 91 6.5 92 6.5 93 2.5 94 2.0 95 1.5 96 1.0 97 2.5 98 3.5 99 4.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10444912697404043 2 0.036471755482244184 3 0.04768561880767797 4 0.05404014135875712 5 0.05815189124474951 6 0.0038981524893174605 7 0.0023495713634242225 8 0.0031505616009552075 9 0.007475908883622527 10-11 0.06824436823763992 12-13 0.00766280660571309 14-15 0.013082840546339422 16-17 0.02437680289552631 18-19 0.021680135762505326 20-21 0.14073398473419407 22-23 0.01879657090739378 24-25 0.017995580669862798 26-27 0.008303598795737877 28-29 0.005660331011885627 30-31 0.0023495713634242225 32-33 0.007475908883622527 34-35 0.017541686201928572 36-37 0.003257360299292672 38-39 0.010573071135409002 40-41 0.03342799257962644 42-43 0.01679409531356632 44-45 0.11504889778403381 46-47 0.004538944679342248 48-49 0.012068252912133506 50-51 0.013563434688858013 52-53 0.014818319394323222 54-55 0.012335249657977169 56-57 0.007502608558206892 58-59 0.016820794988150683 60-61 0.03348139192879517 62-63 0.009024490009515765 64-65 0.007983202700725483 66-67 0.0106531701591621 68-69 0.009798780572462383 70-71 0.004886040448939009 72-73 9.344886104528158E-4 74-75 0.0012548847054652099 76-77 0.009585183175787453 78-79 0.021813634135427157 80-81 0.0038180534655643614 82-83 0.005179736869367036 84-85 0.0031505616009552075 86-87 0.004512245004757882 88-89 0.0069686150665195686 90-91 0.005606931662716895 92-93 0.0033107596484614047 94-95 0.004699142726848445 96-97 0.0019490762446587302 98-99 0.0037112547672268972 100-101 0.0036311557434737986 102-103 7.742905629466188E-4 104-105 0.004218548584329854 106-107 0.0012281850308808436 108-109 0.001762178522568167 110-111 0.0033374593230457708 112-113 0.005126337520198304 114-115 0.0031505616009552075 116-117 0.0058739284085605565 118-119 0.003844753140148728 120-121 0.007689506280297456 122-123 0.004912740123523374 124-125 0.05590911857966275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1872682.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.57787714966662 #Duplication Level Percentage of deduplicated Percentage of total 1 87.18026949966756 64.14539159121368 2 7.798877961068866 11.476497690495352 3 2.241651237093217 4.948078180057288 4 0.9610351176998313 2.828436953065344 5 0.5050943576912076 1.858188529959672 6 0.286151831837576 1.263266659745834 7 0.1926383753157328 0.9921745899304653 8 0.1355504525055694 0.7978811633629205 9 0.10353950235108379 0.6856395105713096 >10 0.5475454143319004 7.280218509296238 >50 0.03385838358050649 1.6881119964253177 >100 0.013413366998577512 1.5592494474658676 >500 7.489997165712567E-5 0.034553850325146435 >1k 2.9959988662850266E-4 0.4423113280855268 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2850 0.15218814513088713 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2043 0.10909487035172015 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.3399349168732334E-5 0.0 0.0 0.0 0.0 3 5.3399349168732334E-5 0.0 0.0 0.0 0.0 4 5.3399349168732334E-5 0.0 0.0 0.0 0.0 5 1.0679869833746467E-4 0.0 0.0 0.0 0.0 6 1.0679869833746467E-4 0.0 0.0 0.0 0.0 7 2.1359739667492934E-4 0.0 0.0 0.0 0.0 8 2.1359739667492934E-4 0.0 0.0 0.0 0.0 9 2.1359739667492934E-4 0.0 0.0 0.0 0.0 10-11 2.1359739667492934E-4 0.0 0.0 0.0 0.0 12-13 2.1359739667492934E-4 0.0 0.0 0.0 0.0 14-15 2.1359739667492934E-4 0.0 0.0 0.0 0.0 16-17 2.936964204280278E-4 0.0 0.0 1.0679869833746467E-4 0.0 18-19 4.5389446793422483E-4 0.0 0.0 1.3349837292183083E-4 0.0 20-21 5.873928408560556E-4 0.0 0.0 3.7379544418112635E-4 0.0 22-23 5.873928408560556E-4 0.0 0.0 6.140925154404218E-4 0.0 24-25 0.0010679869833746466 0.0 0.0 0.0012548847054652096 0.0 26-27 0.0010679869833746466 0.0 0.0 0.001628680149646336 0.0 28-29 0.0014150827529714068 0.0 0.0 0.002429670387177321 0.0 30-31 0.0015485811258932377 0.0 0.0 0.004218548584329854 0.0 32-33 0.0015485811258932377 0.0 0.0 0.008383697819490978 0.0 34-35 0.0017087791733994347 0.0 0.0 0.013723632736364209 0.0 36-37 0.0020291752684118286 0.0 0.0 0.024003007451345183 0.0 38-39 0.002242772665086758 0.0 0.0 0.04170489170077995 0.0 40-41 0.002242772665086758 0.0 0.0 0.0656544998029564 0.0 42-43 0.002402970712592955 0.0 0.0 0.08864291962009567 0.0 44-45 0.002776766156774081 0.0 0.0 0.11184493683390986 0.0 46-47 0.0031238619263708415 0.0 0.0 0.14367094893847435 0.0 48-49 0.0033107596484614047 0.0 0.0 0.17934171418318753 0.0 50-51 0.0033107596484614047 0.0 0.0 0.22147380067731734 0.0 52-53 0.0033374593230457708 0.0 0.0 0.2640864813139657 0.0 54-55 0.003364158997630137 0.0 0.0 0.3103837170432566 0.0 56-57 0.003577756394305066 0.0 0.0 0.36471755482244184 0.0 58-59 0.0036311557434737986 0.0 0.0 0.42724819269902736 0.0 60-61 0.0036311557434737986 0.0 0.0 0.501740284789409 5.3399349168732334E-5 62-63 0.0036311557434737986 0.0 0.0 0.5807179222099641 5.3399349168732334E-5 64-65 0.0037379544418112633 0.0 0.0 0.6604698501934658 5.3399349168732334E-5 66-67 0.00389815248931746 0.0 0.0 0.7523701301128541 5.3399349168732334E-5 68-69 0.00389815248931746 0.0 0.0 0.8589285313790596 5.3399349168732334E-5 70-71 0.003978251513070559 0.0 0.0 0.9910652208970877 5.3399349168732334E-5 72-73 0.004165149235161121 0.0 0.0 1.126646168436499 5.3399349168732334E-5 74-75 0.004298647608082953 0.0 0.0 1.2589430560020334 5.3399349168732334E-5 76-77 0.004325347282667319 0.0 0.0 1.4085680323728216 5.3399349168732334E-5 78-79 0.004352046957251685 0.0 0.0 1.5892714299598116 5.3399349168732334E-5 80-81 0.004859340774354642 0.0 0.0 1.7888514974779488 5.3399349168732334E-5 82-83 0.004912740123523374 0.0 0.0 2.006400445991364 5.3399349168732334E-5 84-85 0.005099637845613937 0.0 0.0 2.2181822647945566 5.3399349168732334E-5 86-87 0.005313235242288867 0.0 0.0 2.437840487600137 5.3399349168732334E-5 88-89 0.005339934916873233 0.0 0.0 2.6878562404081423 5.3399349168732334E-5 90-91 0.005606931662716895 0.0 0.0 2.9732757617150165 5.3399349168732334E-5 92-93 0.005660331011885627 0.0 0.0 3.2881450240884464 5.3399349168732334E-5 94-95 0.005660331011885627 0.0 0.0 3.602106497526008 5.3399349168732334E-5 96-97 0.005660331011885627 0.0 0.0 3.910728036046697 5.3399349168732334E-5 98-99 0.005954027432313655 0.0 0.0 4.249840602942731 5.3399349168732334E-5 100-101 0.006301123201910415 0.0 0.0 4.6499352265894585 5.3399349168732334E-5 102-103 0.006701618320675907 2.6699674584366167E-5 0.0 5.0801470831673505 5.3399349168732334E-5 104-105 0.007849704327803653 5.3399349168732334E-5 0.0 5.513723098742872 5.3399349168732334E-5 106-107 0.008063301724478581 5.3399349168732334E-5 0.0 5.935257561080846 5.3399349168732334E-5 108-109 0.00814340074823168 5.3399349168732334E-5 0.0 6.37104964964687 5.3399349168732334E-5 110-111 0.008383697819490976 5.3399349168732334E-5 0.0 6.864699933037216 5.3399349168732334E-5 112-113 0.008543895866997173 5.3399349168732334E-5 0.0 7.402591577213856 5.3399349168732334E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6740 0.0 49.64004 1 GGTATCA 3870 0.0 44.303417 1 ATCAACG 7645 0.0 41.48441 3 TCAACGC 7710 0.0 40.18292 4 CAACGCA 7845 0.0 39.79522 5 TATCAAC 8530 0.0 39.13353 2 AACGCAG 8025 0.0 37.99037 6 GTACATG 9730 0.0 34.997654 1 TACATGG 9825 0.0 33.06704 2 ACGCAGA 9385 0.0 31.725433 7 CGCAGAG 9305 0.0 31.361586 8 ACATGGG 10035 0.0 30.003254 3 GCAGAGT 9680 0.0 27.93179 9 CATGGGA 6960 0.0 25.937368 4 GAGTACT 4060 0.0 25.274143 12-13 CGCATAG 225 1.896151E-7 23.822002 8 ACGCATA 230 2.3467692E-7 23.301641 7 GTACTTT 4585 0.0 22.678406 14-15 CAGAGTA 9825 0.0 22.2635 10-11 CATGGGG 3520 0.0 22.003574 4 >>END_MODULE