FastQCFastQC Report
Thu 26 May 2016
SRR1364485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872682
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70490.37641201229039417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67170.35868342836637507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39490.21087402986732398No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.10124516602391649No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.057.4459231
GTATCAA93350.050.7551881
TATCAAC122600.038.0160752
ATCAACG117950.037.84733
TCAACGC118850.037.5105484
CAACGCA119350.037.2386975
AACGCAG122600.035.5235946
GTACATG101600.032.126831
CGCAGAG142650.030.8919688
TACATGG102400.030.7897932
ACGCAGA142050.030.5339227
ACATGGG101600.028.3924373
GCAGAGT150750.027.8908169
ATGGGCG3450.027.5928925
GAGTACT86650.027.04341712-13
AGTACTT91100.025.49391712-13
GTACTTT96300.024.70401814-15
AGCGTAC2201.540484E-724.3397485
AGAGTAC141750.024.3354210-11
CAGAGTA146200.024.14389210-11
Produced by FastQC (version 0.11.5)