##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364485_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1872682 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.90111455121585 31.0 28.0 33.0 25.0 34.0 2 29.034455930051124 31.0 28.0 33.0 25.0 34.0 3 29.105657554245727 31.0 28.0 34.0 25.0 34.0 4 32.5112608547527 35.0 32.0 37.0 26.0 37.0 5 31.645626433105033 35.0 32.0 37.0 19.0 37.0 6 31.708894515993638 35.0 32.0 37.0 19.0 37.0 7 31.152652719468655 35.0 31.0 37.0 17.0 37.0 8 31.269483019540957 35.0 31.0 37.0 17.0 37.0 9 31.817257815261748 37.0 30.0 39.0 11.0 39.0 10-11 32.00103995232506 37.0 30.5 39.0 14.0 39.0 12-13 31.766489185029812 37.0 30.0 39.0 11.5 39.0 14-15 32.66333205530891 37.0 31.0 40.0 11.0 41.0 16-17 32.731602856224384 37.5 31.0 40.0 11.0 41.0 18-19 32.64809001207893 37.5 31.0 40.0 10.0 41.0 20-21 32.57482717300641 38.0 30.5 40.0 10.0 41.0 22-23 32.53917109258272 37.0 30.5 40.0 10.0 41.0 24-25 32.40334397404365 37.0 30.0 40.0 10.0 41.0 26-27 32.423406910516576 37.0 30.5 40.0 10.0 41.0 28-29 32.286594573985326 37.0 30.0 40.0 9.0 41.0 30-31 31.764508603169144 37.0 30.0 40.0 8.0 41.0 32-33 31.66351334609934 36.5 30.0 40.0 7.5 41.0 34-35 31.495968349137762 36.0 30.0 40.0 2.0 41.0 36-37 31.418334773335783 36.0 30.0 40.0 2.0 41.0 38-39 31.203600237520305 36.0 30.0 40.0 2.0 41.0 40-41 30.888855128633693 36.0 28.5 40.0 2.0 41.0 42-43 30.76278914412591 36.0 28.5 40.0 2.0 41.0 44-45 30.650565071912904 36.0 28.5 40.0 2.0 41.0 46-47 30.435244745237043 36.0 28.0 40.0 2.0 41.0 48-49 30.11038660060811 36.0 27.0 40.0 2.0 41.0 50-51 29.78924264771061 35.0 27.0 40.0 2.0 41.0 52-53 29.682539801204904 35.0 27.0 40.0 2.0 41.0 54-55 29.22052249127188 35.0 25.5 39.5 2.0 41.0 56-57 29.24499194203821 35.0 26.0 39.0 2.0 41.0 58-59 29.046832030211213 35.0 26.0 39.0 2.0 41.0 60-61 28.79547114779765 34.5 25.5 39.0 2.0 41.0 62-63 28.54021958880365 34.0 25.0 39.0 2.0 40.5 64-65 28.07093462744876 34.0 23.0 38.5 2.0 40.0 66-67 27.73285613894938 34.0 22.5 38.0 2.0 40.0 68-69 27.542648191203845 34.0 22.0 37.5 2.0 40.0 70-71 27.605634859522333 34.0 23.5 37.0 2.0 40.0 72-73 27.39434645070546 34.0 23.0 37.0 2.0 39.5 74-75 27.035162403440626 34.0 22.0 36.0 2.0 39.0 76-77 26.917122341112908 33.0 22.5 36.0 2.0 39.0 78-79 26.51261372726389 33.0 21.5 35.5 2.0 38.5 80-81 26.290078080528353 33.0 21.0 35.0 2.0 37.0 82-83 26.12372282106626 33.0 21.0 35.0 2.0 37.0 84-85 25.711479845483645 33.0 19.5 35.0 2.0 36.0 86-87 25.57119735224667 33.0 20.0 35.0 2.0 36.0 88-89 25.493211607736924 33.0 20.0 35.0 2.0 36.0 90-91 25.31039306192936 32.0 18.5 35.0 2.0 35.5 92-93 25.324293980504965 32.5 19.5 35.0 2.0 35.0 94-95 25.301357625053264 33.0 19.0 35.0 2.0 35.0 96-97 25.172235595792557 33.0 18.0 35.0 2.0 35.0 98-99 25.057315924433514 32.0 18.0 35.0 2.0 35.0 100-101 24.96805864530123 32.0 17.5 35.0 2.0 35.0 102-103 23.83576683067387 30.5 13.0 34.0 2.0 35.0 104-105 24.74907859423009 32.0 17.0 35.0 2.0 35.0 106-107 24.87609508715308 32.5 16.0 35.0 2.0 35.0 108-109 24.930421449023378 33.0 16.5 35.0 2.0 35.0 110-111 24.86051342406239 33.0 13.0 35.0 2.0 35.0 112-113 24.79124165234674 33.0 9.0 35.0 2.0 35.0 114-115 24.728442949737328 33.0 7.0 35.0 2.0 35.0 116-117 24.645328197739925 33.0 2.0 35.0 2.0 35.0 118-119 24.522968128064456 33.0 2.0 35.0 2.0 35.0 120-121 24.352198611403324 33.0 2.0 35.0 2.0 35.0 122-123 24.05833958995708 32.5 2.0 35.0 2.0 35.0 124-125 23.30218584895887 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7581768599181657 1101 2 0.7849178717939509 1101 3 0.6643400576549894 1101 4 0.8579178546125874 1101 5 0.9965833338759253 1101 6 0.9529497274255085 1101 7 1.4048316816113768 1101 8 1.2594690253937486 1101 9 1.981009600380979 1101 10-11 1.551355677297849 1101 12-13 1.831763792532854 1101 14-15 1.6631236676618926 1101 16-17 1.5240967607860796 1101 18-19 1.634254367195382 1101 20-21 1.8191542133185692 1101 22-23 1.764723061672953 1101 24-25 1.8814323954355814 1101 26-27 1.6291214431517886 1101 28-29 1.6966849469042984 1101 30-31 2.2857120058449816 1101 32-33 2.0324990781346486 1101 34-35 2.114020983712006 1101 36-37 1.8900518970429125 1101 38-39 2.141192642327038 1101 40-41 2.1701214963985187 1101 42-43 2.1550200786748626 1101 44-45 1.957506692796752 1101 46-47 1.7528213019137766 1101 48-49 1.8108814019182482 1101 50-51 2.07822375693074 1101 52-53 2.154300239087931 1101 54-55 2.4098894115790834 1101 56-57 2.176415114441067 1101 58-59 2.3302308957007405 1101 60-61 2.445482852174358 1101 62-63 2.3402119524081435 1101 64-65 2.514396833879708 1101 66-67 2.712979909123348 1101 68-69 2.5198698009962825 1101 70-71 2.0949825802551985 1101 72-73 2.396044155082997 1101 74-75 2.3978895974027203 1101 76-77 2.1529180950153517 1101 78-79 2.26883472023561 1101 80-81 2.3130620518358427 1101 82-83 2.2593375446160735 1101 84-85 2.5069565855146116 1101 86-87 2.306727111809405 1101 88-89 2.20117733853456 1101 90-91 2.2576993556867713 1101 92-93 2.0656403133710235 1101 94-95 2.087211607141633 1101 96-97 1.9882406181367926 1101 98-99 2.128366432779231 1101 100-101 2.1120232021168057 1101 102-103 1.9948472238871524 1101 104-105 2.0174791658224187 1101 106-107 2.0267024227458315 1101 108-109 1.964541436434736 1101 110-111 2.0754270071414886 1101 112-113 1.9955185897596657 1101 114-115 1.971141661493121 1101 116-117 2.0627821318105752 1101 118-119 1.9419143792238849 1101 120-121 1.819105038092733 1101 122-123 1.9457076310968624 1101 124-125 1.7974887695778499 1102 1 0.41570841860738383 1102 2 -1.7035617661458495 1102 3 -0.124595593307685 1102 4 0.4167550927865662 1102 5 0.8605907868740985 1102 6 0.5344962473895443 1102 7 1.2896728319926538 1102 8 0.995677820813313 1102 9 1.6796752994461883 1102 10-11 1.4722366126367916 1102 12-13 1.6164979379798439 1102 14-15 1.5640503927524136 1102 16-17 1.535019281744212 1102 18-19 1.697767831783075 1102 20-21 1.809219509285029 1102 22-23 1.716391471781094 1102 24-25 2.0056016909698684 1102 26-27 1.629251301845656 1102 28-29 1.635031819462661 1102 30-31 2.019312377535517 1102 32-33 1.7241767919272206 1102 34-35 1.9914655564923258 1102 36-37 1.4920918777594103 1102 38-39 1.4518895038240025 1102 40-41 1.6197395211050143 1102 42-43 1.4513948510937915 1102 44-45 1.3969340650678177 1102 46-47 1.211035241199525 1102 48-49 1.089929918017127 1102 50-51 1.2762755110453021 1102 52-53 1.107264495054153 1102 54-55 1.5595918158836746 1102 56-57 1.226115759885598 1102 58-59 1.4382155949887796 1102 60-61 1.261128163653055 1102 62-63 1.4060710396214482 1102 64-65 1.6245576656423495 1102 66-67 1.871407915383518 1102 68-69 1.739182575539779 1102 70-71 1.1776416363115523 1102 72-73 1.606863634252555 1102 74-75 1.751000227073476 1102 76-77 1.638606970259751 1102 78-79 1.52174994512243 1102 80-81 1.546056416288323 1102 82-83 1.1291531190020905 1102 84-85 1.1760629281352983 1102 86-87 1.40533628750822 1102 88-89 1.4141010539185679 1102 90-91 1.2133334241664997 1102 92-93 1.251724109804215 1102 94-95 1.2621134507581857 1102 96-97 1.3141340305320277 1102 98-99 1.1532925635120996 1102 100-101 0.9202550897699631 1102 102-103 0.6821362392007693 1102 104-105 1.102813345235969 1102 106-107 1.4108922809531599 1102 108-109 1.2941281123160024 1102 110-111 1.1290073095534936 1102 112-113 1.1730596942621077 1102 114-115 1.4504269726931405 1102 116-117 0.9980572238052403 1102 118-119 0.5198074955824126 1102 120-121 0.889731549755858 1102 122-123 1.177951624732529 1102 124-125 1.2486844574925726 1103 1 0.6213370503484121 1103 2 0.6307934568109452 1103 3 0.3859130460213578 1103 4 0.5457066935738979 1103 5 0.8407304035824126 1103 6 0.8226919683305915 1103 7 1.1164407321964092 1103 8 0.9114965475637646 1103 9 1.7981736478759345 1103 10-11 1.4305224343336675 1103 12-13 1.3931324940498016 1103 14-15 1.234155898009675 1103 16-17 1.16659137861879 1103 18-19 1.3091586471000696 1103 20-21 1.5568889293765196 1103 22-23 1.364115101870901 1103 24-25 1.7085506317978911 1103 26-27 1.2172020046717051 1103 28-29 1.2911742692145651 1103 30-31 1.9678424765466467 1103 32-33 1.580517353952672 1103 34-35 1.7637299074297097 1103 36-37 1.7842896535570993 1103 38-39 1.8014358152940169 1103 40-41 1.9203012488462043 1103 42-43 2.056999458735941 1103 44-45 1.9053695008213474 1103 46-47 1.7594173185294366 1103 48-49 1.7179901347324993 1103 50-51 1.8741480974714797 1103 52-53 1.6478904924523476 1103 54-55 2.030590332205179 1103 56-57 1.7559646642274522 1103 58-59 1.8755459291176066 1103 60-61 1.8170139640259464 1103 62-63 1.8786427190167352 1103 64-65 2.0630017971504024 1103 66-67 2.2518689842274497 1103 68-69 2.3612630763487736 1103 70-71 2.1068484325698833 1103 72-73 2.1434747685569633 1103 74-75 2.1444787919693553 1103 76-77 1.938056631464569 1103 78-79 2.1838213970849623 1103 80-81 2.1316659810644225 1103 82-83 1.9899348507172938 1103 84-85 2.18423631428011 1103 86-87 2.072923070238282 1103 88-89 1.9557582874916584 1103 90-91 2.0222684504026205 1103 92-93 1.7159508765274438 1103 94-95 1.66874659446038 1103 96-97 1.6163211928013155 1103 98-99 1.8370069938259448 1103 100-101 1.8961229863043307 1103 102-103 1.716180706630805 1103 104-105 1.7006897395164344 1103 106-107 1.6803177034345929 1103 108-109 1.6979699257408356 1103 110-111 1.684125481118791 1103 112-113 1.5529705281614241 1103 114-115 1.4098868251007097 1103 116-117 1.6151759173927438 1103 118-119 1.7766389592592944 1103 120-121 1.661266177344249 1103 122-123 1.8460375825428876 1103 124-125 1.5719262846256825 1104 1 0.3583991866678282 1104 2 0.3495843332950166 1104 3 0.30545857035729895 1104 4 0.2846949874823892 1104 5 0.5283680039182173 1104 6 0.7921765712607503 1104 7 1.1136121206838752 1104 8 0.8460882423518044 1104 9 1.2826865193487933 1104 10-11 1.0232318652572232 1104 12-13 1.0791604052132868 1104 14-15 1.0487101283846272 1104 16-17 0.8574199119340236 1104 18-19 0.8510308836987335 1104 20-21 1.2720615334365846 1104 22-23 1.2555182754950351 1104 24-25 1.4696978652280208 1104 26-27 1.28141977799487 1104 28-29 1.20159212613855 1104 30-31 1.6926479003639194 1104 32-33 1.70495292212523 1104 34-35 1.656813773811617 1104 36-37 1.5629464919656861 1104 38-39 1.6789036703838853 1104 40-41 1.9050582123045636 1104 42-43 1.8052059180523692 1104 44-45 1.487077878936919 1104 46-47 1.4538311210553374 1104 48-49 1.295621929577937 1104 50-51 1.7089854108495643 1104 52-53 1.4896004916242482 1104 54-55 1.8396822287466392 1104 56-57 1.771741023910419 1104 58-59 1.9251864961183038 1104 60-61 2.0606553266182495 1104 62-63 2.0536293804468144 1104 64-65 2.1626343247660174 1104 66-67 2.1408567180148523 1104 68-69 2.3920710543182224 1104 70-71 2.1356896167141954 1104 72-73 2.2695061876160594 1104 74-75 2.2807688288209285 1104 76-77 2.1163150590355286 1104 78-79 2.1550220023671898 1104 80-81 2.0171407671585726 1104 82-83 1.953326873758261 1104 84-85 2.149527037993902 1104 86-87 2.1654527681880396 1104 88-89 1.856984776957976 1104 90-91 1.969499881674551 1104 92-93 1.707603150278608 1104 94-95 1.6346961743366109 1104 96-97 1.6534775392917815 1104 98-99 1.7013425902681405 1104 100-101 1.646225180946633 1104 102-103 1.6184867471304152 1104 104-105 1.5589423110762972 1104 106-107 1.6324292171739216 1104 108-109 1.5121989014858315 1104 110-111 1.5858688744905152 1104 112-113 1.5943013692853825 1104 114-115 1.4887983318989342 1104 116-117 1.5081911963978456 1104 118-119 1.4746831701661876 1104 120-121 1.5805858960009154 1104 122-123 1.6732407749255955 1104 124-125 1.4628864134491408 1105 1 0.33054919646615843 1105 2 0.3677580303376864 1105 3 0.2313378381483311 1105 4 0.4064580073456412 1105 5 0.5727409813573416 1105 6 0.6595763546381939 1105 7 0.9027978308333573 1105 8 0.8579872559864938 1105 9 1.3805781348022634 1105 10-11 0.9793128881853193 1105 12-13 1.1000582148008178 1105 14-15 1.0999285861449906 1105 16-17 1.1068847001753213 1105 18-19 1.2753273569175079 1105 20-21 1.465796604222902 1105 22-23 1.3742167790868791 1105 24-25 1.5362838685329763 1105 26-27 1.3413341992842618 1105 28-29 1.3062844232380684 1105 30-31 1.588452526500216 1105 32-33 1.4348562474668967 1105 34-35 1.598432383048884 1105 36-37 1.320048461253652 1105 38-39 1.3974468902919703 1105 40-41 1.566806366952239 1105 42-43 1.6548772325999757 1105 44-45 1.5126203511705647 1105 46-47 1.3201108823821173 1105 48-49 1.5522357477844686 1105 50-51 1.6085323814881143 1105 52-53 1.452746133220721 1105 54-55 1.6825920329035995 1105 56-57 1.3779274009643174 1105 58-59 1.3937094683493036 1105 60-61 1.3234317212032138 1105 62-63 1.3261478869340166 1105 64-65 1.6490062588153052 1105 66-67 1.834292928673669 1105 68-69 1.9469416523518461 1105 70-71 1.7661712752979781 1105 72-73 1.9720903760229547 1105 74-75 1.9172058423249716 1105 76-77 1.7694297586504817 1105 78-79 1.8849049696901972 1105 80-81 1.8717999066179516 1105 82-83 1.7753684398669805 1105 84-85 2.141526502175264 1105 86-87 2.056585091116311 1105 88-89 1.9555779440786623 1105 90-91 2.0062358987501305 1105 92-93 1.7955761624973938 1105 94-95 1.750245695189399 1105 96-97 1.692456800374046 1105 98-99 1.6715655949836972 1105 100-101 1.7766037805091486 1105 102-103 1.5581696343013114 1105 104-105 1.3236762753574105 1105 106-107 1.538675631073076 1105 108-109 1.4640171539226152 1105 110-111 1.6100100088547613 1105 112-113 1.6261413656308719 1105 114-115 1.5525960590392494 1105 116-117 1.4959782610121017 1105 118-119 1.5435207482659479 1105 120-121 1.5720983676872713 1105 122-123 1.6141528671252416 1105 124-125 1.4761120085432644 1106 1 0.2135158652089828 1106 2 0.15979269626879855 1106 3 0.17841390121612477 1106 4 0.04055736937149845 1106 5 0.2026076474308205 1106 6 0.3646210884597174 1106 7 0.4799011657386387 1106 8 0.32551842062297 1106 9 0.9547592011177599 1106 10-11 0.5279786035260585 1106 12-13 0.46818887048164015 1106 14-15 0.45905230597023206 1106 16-17 0.37918604508175235 1106 18-19 0.5234271705080502 1106 20-21 0.4535718807228619 1106 22-23 0.33509737470736667 1106 24-25 0.12017596681836551 1106 26-27 0.19392897225622363 1106 28-29 0.2775353241429741 1106 30-31 0.8073210900351491 1106 32-33 0.4456274554357833 1106 34-35 0.5005359403998249 1106 36-37 0.3723450458230424 1106 38-39 0.5656208672643999 1106 40-41 0.6907515296489883 1106 42-43 0.6199623847973008 1106 44-45 0.5376346433039991 1106 46-47 0.5880742033178166 1106 48-49 0.5022678355704251 1106 50-51 0.9297406284383847 1106 52-53 0.5436506083824426 1106 54-55 1.0346380880523327 1106 56-57 0.7456226748839008 1106 58-59 0.7548505478335876 1106 60-61 1.0351433181850709 1106 62-63 1.090882687222308 1106 64-65 1.1195700250872065 1106 66-67 1.2310016783511237 1106 68-69 1.1849581155548847 1106 70-71 0.9744190041278245 1106 72-73 1.0041526363377642 1106 74-75 1.3161807015168705 1106 76-77 0.8868673848883901 1106 78-79 0.7359046404706255 1106 80-81 1.0242493113533762 1106 82-83 0.9082143206183133 1106 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-4.049314423586765 2216 94-95 -3.7901241618271726 2216 96-97 -3.9231508791871192 2216 98-99 -3.867174628264049 2216 100-101 -4.340254457843617 2216 102-103 -4.231350796463989 2216 104-105 -4.1730582599800705 2216 106-107 -4.313226739211647 2216 108-109 -4.203312780165184 2216 110-111 -3.850901573508601 2216 112-113 -3.9609510316102927 2216 114-115 -3.878712908179427 2216 116-117 -3.803080070108475 2216 118-119 -3.9956923471201975 2216 120-121 -3.9874710224652254 2216 122-123 -4.017668171672543 2216 124-125 -3.8930546063056326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96119.0 3 31232.0 4 14892.0 5 12187.0 6 12863.0 7 15309.0 8 17079.0 9 17688.0 10 18903.0 11 20422.0 12 22457.0 13 24867.0 14 26189.0 15 21515.0 16 15168.0 17 12791.0 18 11476.0 19 10535.0 20 10227.0 21 10744.0 22 13250.0 23 17635.0 24 24373.0 25 32282.0 26 39696.0 27 44230.0 28 49575.0 29 56989.0 30 63636.0 31 71206.0 32 83190.0 33 99289.0 34 121474.0 35 151134.0 36 207688.0 37 272163.0 38 101666.0 39 543.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11933019090821 20.43365680569528 14.488582605190881 22.958430398205632 2 15.621852769135966 21.431126974311653 39.56505069211044 23.38196956444194 3 19.104791950795704 26.56590921469849 29.73003425034256 24.59926458416325 4 12.51684528869575 18.142088371762082 37.186640181423556 32.15442615811862 5 12.524550350780325 39.50916386231085 33.931601841636756 14.034683945272075 6 30.098660848261154 38.43933660778841 17.488484405754754 13.973518138195676 7 26.835118331231296 33.677832852828885 21.005870220529907 18.481178595409915 8 25.66917394410797 36.549184538538846 19.27502907594562 18.50661244140756 9 25.94930692984714 16.349545731736622 20.299922784541103 37.40122455387514 10-11 24.53940145417884 27.95318667657764 26.921879538968916 20.585532330274603 12-13 25.6230327267232 25.31336866070425 27.81508478916117 21.248513823411383 14-15 22.582476248554542 26.217996657723138 26.542585728280205 24.656941365442115 16-17 21.335026879753755 29.586766841165673 27.03886416686114 22.039342112219433 18-19 21.04470024329133 27.615114821119995 30.35110100541905 20.98908393016962 20-21 22.214965588246603 26.580436138535614 30.51746861075629 20.687129662461498 22-23 21.650931015986096 26.57641529408592 30.37179496421798 21.400858725710002 24-25 21.343082407876157 26.748115442371734 30.526709559176922 21.382092590575184 26-27 21.232388781581108 27.069917264721383 30.40231784808173 21.29537610561578 28-29 21.065419782186826 26.861969418150245 30.72734156638795 21.345269233274983 30-31 21.21261880121709 26.70345379261438 30.641956440710732 21.441970965457802 32-33 21.16325266463037 26.77558365550975 30.47437362496529 21.58679005489459 34-35 21.496072705019536 26.774865525724596 30.403815315409126 21.32524645384674 36-37 21.507225821679228 26.625536202800404 30.34046775200397 21.5267702235164 38-39 21.360532629596975 26.888652625047328 30.387291734639987 21.36352301071571 40-41 21.508816636077267 26.752179928689408 30.273894843305193 21.465108591928136 42-43 21.397874152611386 26.803405841407834 30.04482916058131 21.75389084539947 44-45 21.552949343514914 27.012956152002833 29.771248156034524 21.662846348447733 46-47 21.64568601756415 27.27551132656015 29.180483777777066 21.89831887809864 48-49 21.870370943918935 27.29526422531962 28.82900033214395 22.005364498617492 50-51 21.83450588448036 27.503645330144444 28.48608818079662 22.175760604578574 52-53 21.987686096930602 27.66633915030865 28.325127624580816 22.020847128179934 54-55 21.784730376901244 27.509475469656035 28.43579353315238 22.27000062029034 56-57 21.800105624081866 27.73080704167666 28.318948415612073 22.15013891862941 58-59 21.896536625011613 27.428762598241452 28.3985481784948 22.276152598252132 60-61 22.071499592562965 27.482615841878122 28.53727968763517 21.90860487792375 62-63 21.84834191553998 27.478823051328234 28.61108159299568 22.061753440136105 64-65 21.70849405891974 27.220390913836834 28.62859551975702 22.4425195074864 66-67 21.705176869520066 27.578419044709086 28.302114571236576 22.41428951453427 68-69 21.776734576506236 27.399021015355473 28.433782099153902 22.390462308984386 70-71 21.667805440320915 27.33983275380034 28.632427627796968 22.359934178081772 72-73 21.78151924208362 27.401499780104217 28.28404871159238 22.532932266219785 74-75 21.662126177276406 27.599420163083803 28.217038863972494 22.521414795667297 76-77 21.819097390674223 27.432056790155762 28.32376177376587 22.42508404540414 78-79 21.84972829917849 27.499962619776912 28.197450882386864 22.45285819865773 80-81 21.613255231340975 27.693366643891814 28.203411208378153 22.489966916389058 82-83 21.754591880462257 27.71874326667042 28.08450240177115 22.44216245109617 84-85 21.72478921930644 27.466423806296962 27.984744865783334 22.824042108613266 86-87 21.631201791875736 27.448042892665008 28.208612868920895 22.712142446538362 88-89 21.83115946537026 27.34841686466928 28.173419581392356 22.64700408856811 90-91 21.704202514422537 27.559404947944827 28.242571527970796 22.49382100966184 92-93 21.686854754173122 27.50797137088355 28.202979858904996 22.60219401603833 94-95 21.577831843615396 27.383726705023033 28.157351167939577 22.881090283421997 96-97 21.6979643406502 27.381822366487036 28.10468021957278 22.815533073289984 98-99 21.38753402873526 27.252598145333806 28.0556175581332 23.304250267797734 100-101 21.425985338471136 27.430825522680085 28.174982484770293 22.968206654078486 102-103 21.591258618774617 27.25233571996138 28.207529839970608 22.948875821293395 104-105 21.50174976924726 27.199266503993886 28.225632257163614 23.07335146959524 106-107 21.246176269790816 27.293604007731165 28.296177591425764 23.164042131052256 108-109 21.44931707572348 27.353976809730646 27.83061940041075 23.36608671413513 110-111 21.17194293835588 27.39616265809925 28.041842784912397 23.390051618632477 112-113 21.38200333212295 27.35990665783796 28.044406946194762 23.21368306384433 114-115 21.235877506938593 27.423541960641916 27.8938845583396 23.446695974079894 116-117 21.136530387967632 27.356620077514492 28.0137791680595 23.493070366458372 118-119 21.139336694344006 27.36169206114754 27.88031512545584 23.618656119052616 120-121 21.013254086498666 27.41094076245708 27.969566865851768 23.606238285192482 122-123 21.129533227761495 27.479436320076513 27.74638301644427 23.644647435717726 124-125 20.967390503289145 27.315994151203565 27.859565868919027 23.85704947658826 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 344.0 1 319.5 2 523.5 3 788.0 4 584.5 5 408.0 6 817.5 7 1830.0 8 2891.5 9 3364.0 10 2778.5 11 1823.0 12 1522.5 13 1569.0 14 1746.5 15 1963.0 16 2157.0 17 2458.0 18 2808.0 19 3216.5 20 3695.0 21 4348.5 22 5206.5 23 6215.0 24 7490.0 25 9298.5 26 11958.5 27 15087.0 28 18034.5 29 21276.5 30 25368.5 31 29618.5 32 34257.5 33 38811.5 34 43492.5 35 47982.5 36 52452.5 37 56984.5 38 59074.0 39 59670.0 40 60028.0 41 61233.0 42 62426.0 43 63663.0 44 65933.0 45 67099.0 46 66572.5 47 65702.5 48 67016.5 49 67680.0 50 65955.0 51 63189.5 52 60234.5 53 57757.5 54 54790.0 55 50611.5 56 45643.5 57 41459.5 58 38332.0 59 34651.5 60 29723.5 61 24072.0 62 18685.5 63 14196.0 64 10451.5 65 7846.5 66 5877.5 67 4426.0 68 3355.0 69 2428.5 70 1797.5 71 1431.0 72 1102.0 73 939.0 74 760.5 75 543.0 76 391.0 77 226.0 78 133.5 79 78.5 80 52.0 81 43.0 82 24.5 83 17.0 84 13.5 85 7.5 86 5.0 87 3.5 88 2.5 89 3.5 90 3.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10359473738734072 2 2.135973966749293E-4 3 0.0 4 0.18502874486965754 5 0.0 6 2.135973966749293E-4 7 0.03689895027559404 8 0.0 9 0.0 10-11 5.339934916873233E-4 12-13 4.004951187654925E-4 14-15 3.4709576959676014E-4 16-17 0.004432145981004784 18-19 0.00160198047506197 20-21 0.003524357045136334 22-23 0.0012548847054652099 24-25 0.005019538821860839 26-27 0.004432145981004784 28-29 0.0036311557434737986 30-31 8.543895866997172E-4 32-33 1.0679869833746465E-4 34-35 0.0010145876342059143 36-37 0.0012281850308808436 38-39 5.873928408560556E-4 40-41 0.0015485811258932375 42-43 9.077889358684497E-4 44-45 0.0010412873087902806 46-47 2.135973966749293E-4 48-49 0.0 50-51 0.04093060113783333 52-53 1.0679869833746465E-4 54-55 0.13830431434701673 56-57 1.60198047506197E-4 58-59 0.0 60-61 0.0 62-63 4.80594142518591E-4 64-65 0.06520060533502219 66-67 0.03332119388128898 68-69 0.0014684821021401391 70-71 0.0028568651805271797 72-73 0.07160852723527006 74-75 0.004619043703095347 76-77 0.0017888781971525333 78-79 0.0018155778717368993 80-81 1.3349837292183083E-4 82-83 8.00990237530985E-5 84-85 5.339934916873233E-5 86-87 4.004951187654925E-4 88-89 0.001148086007127745 90-91 2.936964204280278E-4 92-93 0.00229617201425549 94-95 0.0036311557434737986 96-97 0.002429670387177321 98-99 0.0 100-101 0.0013883830783870405 102-103 0.0013883830783870405 104-105 3.4709576959676014E-4 106-107 8.00990237530985E-5 108-109 0.0 110-111 2.936964204280278E-4 112-113 1.0679869833746465E-4 114-115 2.402970712592955E-4 116-117 0.0 118-119 0.0016553798242307024 120-121 0.002776766156774081 122-123 0.0012548847054652099 124-125 0.008543895866997173 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1872682.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.45960127908756 #Duplication Level Percentage of deduplicated Percentage of total 1 82.4504111830775 53.147206301579985 2 10.228097132536986 13.18598126014226 3 3.2217074789650906 6.230099385958324 4 1.3790851786541212 3.555811229837756 5 0.7363321469074865 2.3731838299315555 6 0.4342682267724187 1.6795654047555897 7 0.291581588794392 1.3156663067806562 8 0.19612527403100247 1.0113725571832166 9 0.16082481213226338 0.9330032939246877 >10 0.8135135948680581 9.629657255765778 >50 0.06040881687844487 2.674094716581728 >100 0.0267177754548635 2.9103112008005914 >500 5.055223241075614E-4 0.21588070935393838 >1k 2.527611620537803E-4 0.3905404228840676 >5k 1.6850744136918686E-4 0.7476261245198268 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7049 0.37641201229039417 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6717 0.35868342836637507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3949 0.21087402986732398 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1896 0.10124516602391649 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.3399349168732334E-5 0.0 0.0 0.0 0.0 3 5.3399349168732334E-5 0.0 0.0 0.0 0.0 4 5.3399349168732334E-5 0.0 0.0 0.0 0.0 5 1.0679869833746467E-4 0.0 0.0 0.0 0.0 6 1.0679869833746467E-4 0.0 0.0 0.0 0.0 7 1.60198047506197E-4 0.0 0.0 0.0 0.0 8 1.60198047506197E-4 0.0 0.0 0.0 0.0 9 1.60198047506197E-4 0.0 0.0 0.0 0.0 10-11 1.60198047506197E-4 0.0 0.0 0.0 0.0 12-13 1.8689772209056318E-4 0.0 0.0 0.0 0.0 14-15 2.402970712592955E-4 0.0 0.0 8.009902375309851E-5 0.0 16-17 2.936964204280278E-4 0.0 0.0 1.60198047506197E-4 0.0 18-19 3.7379544418112635E-4 0.0 0.0 1.8689772209056318E-4 0.0 20-21 4.2719479334985867E-4 0.0 0.0 4.2719479334985867E-4 0.0 22-23 4.2719479334985867E-4 0.0 0.0 8.543895866997173E-4 0.0 24-25 6.40792190024788E-4 0.0 0.0 0.00160198047506197 0.0 26-27 6.40792190024788E-4 0.0 0.0 0.0018956768954899977 0.0 28-29 6.674918646091541E-4 0.0 0.0 0.002696667133020983 0.0 30-31 6.941915391935203E-4 0.0 0.0 0.004405446306420417 0.0 32-33 6.941915391935203E-4 0.0 0.0 0.008437097168659709 0.0 34-35 8.276899121153511E-4 0.0 0.0 0.013750332410948574 0.0 36-37 0.0010679869833746466 0.0 0.0 0.024270004197188846 0.0 38-39 0.001281584380049576 0.0 0.0 0.042585980962064035 0.0 40-41 0.001281584380049576 0.0 0.0 0.0673098796271871 0.0 42-43 0.0014951817767245054 0.0 0.0 0.09152648447520721 0.0 44-45 0.0016553798242307024 0.0 0.0 0.11494209908569633 0.0 46-47 0.0019757759192430965 0.0 0.0 0.1468749098885983 0.0 48-49 0.0020291752684118286 0.0 0.0 0.18412095593378908 0.0 50-51 0.0020291752684118286 0.0 0.0 0.22716083136378734 0.0 52-53 0.0020291752684118286 0.0 0.0 0.27156239019758827 0.0 54-55 0.0020291752684118286 0.0 0.0 0.3198354018461223 0.0 56-57 0.0021359739667492932 0.0 0.0 0.37595811782245997 0.0 58-59 0.0021893733159180258 0.0 0.0 0.43974364040451075 0.0 60-61 0.0021893733159180258 0.0 0.0 0.5160780100412136 0.0 62-63 0.0021893733159180258 0.0 0.0 0.5943881555971595 0.0 64-65 0.0021893733159180258 0.0 0.0 0.6735259910652209 0.0 66-67 0.0022961720142554904 0.0 0.0 0.7673219478800992 0.0 68-69 0.0022961720142554904 0.0 0.0 0.875081834502601 0.0 70-71 0.002402970712592955 0.0 0.0 1.0066578308543574 0.0 72-73 0.0024830697363460537 0.0 0.0 1.1390882167928136 0.0 74-75 0.0025364690855147858 0.0 0.0 1.268234542757393 0.0 76-77 0.002589868434683518 0.0 0.0 1.4148691555747317 0.0 78-79 0.0026165681092678843 0.0 0.0 1.5901525192210957 0.0 80-81 0.00320396095012394 0.0 0.0 1.7839120576798408 0.0 82-83 0.00320396095012394 0.0 0.0 1.9977497514260296 0.0 84-85 0.003390858672214503 0.0 0.0 2.206274209929929 0.0 86-87 0.003604456068889432 0.0 0.0 2.422594973412464 0.0 88-89 0.0036311557434737986 0.0 0.0 2.670634950301226 0.0 90-91 0.004191848909745488 0.0 0.0 2.9583506436223557 0.0 92-93 0.0042719479334985865 0.0 0.0 3.2755427776846258 0.0 94-95 0.0042719479334985865 0.0 0.0 3.592521314350221 0.0 96-97 0.0042719479334985865 0.0 0.0 3.903385625535996 0.0 98-99 0.0047258424014328115 0.0 0.0 4.2494134081493815 0.0 100-101 0.0051263375201983045 0.0 0.0 4.66058839674862 0.0 102-103 0.005580231988132529 0.0 0.0 5.103002004611568 0.0 104-105 0.006140925154404218 0.0 0.0 5.548486075051716 0.0 106-107 0.006301123201910415 0.0 0.0 5.983397074356457 0.0 108-109 0.006381222225663514 0.0 0.0 6.433339990452197 0.0 110-111 0.006488020924000979 0.0 0.0 6.939459021873441 0.0 112-113 0.006514720598585344 0.0 0.0 7.488644628399269 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5360 0.0 57.445923 1 GTATCAA 9335 0.0 50.755188 1 TATCAAC 12260 0.0 38.016075 2 ATCAACG 11795 0.0 37.8473 3 TCAACGC 11885 0.0 37.510548 4 CAACGCA 11935 0.0 37.238697 5 AACGCAG 12260 0.0 35.523594 6 GTACATG 10160 0.0 32.12683 1 CGCAGAG 14265 0.0 30.891968 8 TACATGG 10240 0.0 30.789793 2 ACGCAGA 14205 0.0 30.533922 7 ACATGGG 10160 0.0 28.392437 3 GCAGAGT 15075 0.0 27.890816 9 ATGGGCG 345 0.0 27.592892 5 GAGTACT 8665 0.0 27.043417 12-13 AGTACTT 9110 0.0 25.493917 12-13 GTACTTT 9630 0.0 24.704018 14-15 AGCGTAC 220 1.540484E-7 24.339748 5 AGAGTAC 14175 0.0 24.33542 10-11 CAGAGTA 14620 0.0 24.143892 10-11 >>END_MODULE