FastQCFastQC Report
Thu 26 May 2016
SRR1364484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958550
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76000.049.031391
GGTATCA41600.048.0794371
ATCAACG89900.040.839213
TCAACGC90300.040.761434
CAACGCA91250.040.2704285
AACGCAG91500.039.162846
TATCAAC97900.038.412412
GTACATG123550.035.412621
TACATGG121800.034.4412572
ACGCAGA109250.031.9838057
CGCAGAG109350.030.8134618
ACATGGG125050.030.6446063
GCAGAGT117200.026.3114629
CATGGGG58500.024.2309114
CATGGGA84950.023.5572474
CAGAGTA112400.022.4213110-11
GAGTACT51150.021.98004212-13
AGAGTAC111100.021.93379210-11
GAGTACA96600.021.0747491
AGTACAT90600.021.0164282
Produced by FastQC (version 0.11.5)