##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364484_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2958550 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.719239492318874 30.0 26.0 31.0 7.0 34.0 2 25.997872268509912 30.0 26.0 31.0 7.0 34.0 3 26.020458670632575 30.0 26.0 31.0 7.0 34.0 4 28.786611346774603 35.0 27.0 37.0 7.0 37.0 5 29.379744131415727 35.0 28.0 37.0 7.0 37.0 6 29.457579219550116 35.0 29.0 37.0 2.0 37.0 7 29.26601713677308 35.0 29.0 37.0 2.0 37.0 8 28.882968345980295 35.0 28.0 37.0 2.0 37.0 9 29.503836676750435 35.0 27.0 39.0 2.0 39.0 10-11 29.60851261597742 35.0 27.0 39.0 2.0 39.0 12-13 29.428495884808434 35.0 27.0 39.0 2.0 39.0 14-15 30.034416352605163 36.0 27.0 40.0 2.0 41.0 16-17 30.26357117506887 36.0 27.0 40.0 2.0 41.0 18-19 30.173901404404184 36.0 27.0 40.0 2.0 41.0 20-21 29.938047016274865 36.0 26.5 40.0 2.0 41.0 22-23 29.991297426104005 36.0 27.0 40.0 2.0 41.0 24-25 30.01297527505028 36.0 27.0 40.0 2.0 41.0 26-27 30.109173074648055 36.0 27.0 40.0 2.0 41.0 28-29 30.042423484477194 36.0 27.0 40.0 2.0 41.0 30-31 29.672552939784694 36.0 26.0 40.0 2.0 41.0 32-33 29.689681262780756 36.0 26.0 40.0 2.0 41.0 34-35 29.38316590897568 35.0 25.0 40.0 2.0 41.0 36-37 29.14496476314411 35.0 25.0 40.0 2.0 41.0 38-39 28.99523922191614 35.0 24.5 40.0 2.0 41.0 40-41 29.055177198289705 35.0 25.0 40.0 2.0 41.0 42-43 28.930743607510436 35.0 24.5 40.0 2.0 41.0 44-45 28.576304270673134 35.0 23.5 39.5 2.0 41.0 46-47 28.32534349596931 34.5 23.5 39.0 2.0 41.0 48-49 28.09101519325345 34.0 22.5 39.0 2.0 41.0 50-51 28.08416961011306 34.0 22.5 39.0 2.0 41.0 52-53 27.918617059032293 34.0 22.0 39.0 2.0 40.5 54-55 27.785380507343124 34.0 21.5 39.0 2.0 40.0 56-57 27.39186222980852 33.5 20.0 39.0 2.0 40.0 58-59 27.121367054807255 33.0 18.5 38.5 2.0 40.0 60-61 26.723299251322437 33.0 16.0 38.0 2.0 40.0 62-63 26.646093525544607 33.0 18.0 38.0 2.0 40.0 64-65 26.444369539132346 33.0 16.0 37.5 2.0 40.0 66-67 26.21860472190769 32.0 15.5 37.0 2.0 40.0 68-69 26.034244139865812 32.0 14.5 37.0 2.0 40.0 70-71 25.904551722972403 32.0 13.0 36.0 2.0 39.0 72-73 25.875177536293116 32.0 13.5 36.0 2.0 39.0 74-75 25.58202565445911 32.0 12.5 36.0 2.0 39.0 76-77 25.299633435297697 32.0 10.5 35.0 2.0 38.5 78-79 25.02098629396157 32.0 8.0 35.0 2.0 37.0 80-81 25.013533656689933 32.0 8.0 35.0 2.0 37.0 82-83 24.820161903635224 32.0 7.0 35.0 2.0 36.0 84-85 24.639128627199135 32.0 7.0 35.0 2.0 36.0 86-87 24.443634888712374 32.0 4.0 35.0 2.0 36.0 88-89 24.219367426611008 32.0 2.0 35.0 2.0 35.0 90-91 24.060695104020553 32.0 2.0 35.0 2.0 35.0 92-93 23.95736678440452 32.0 2.0 35.0 2.0 35.0 94-95 23.901879636984333 32.0 2.0 35.0 2.0 35.0 96-97 23.789374693684408 32.0 2.0 35.0 2.0 35.0 98-99 23.691591827077453 32.0 2.0 35.0 2.0 35.0 100-101 23.615588379442634 32.0 2.0 35.0 2.0 35.0 102-103 22.531896537155028 29.5 2.0 33.5 2.0 35.0 104-105 23.30476128508898 31.0 2.0 34.0 2.0 35.0 106-107 23.367781007588178 31.0 2.0 35.0 2.0 35.0 108-109 23.325453854083925 31.5 2.0 35.0 2.0 35.0 110-111 23.214726470737354 31.0 2.0 35.0 2.0 35.0 112-113 23.19074005847459 31.5 2.0 35.0 2.0 35.0 114-115 23.133751837893563 32.0 2.0 35.0 2.0 35.0 116-117 22.949567524632 31.0 2.0 35.0 2.0 35.0 118-119 22.79060232208345 31.0 2.0 35.0 2.0 35.0 120-121 22.626783052508827 31.0 2.0 35.0 2.0 35.0 122-123 22.360085514863698 31.0 2.0 35.0 2.0 35.0 124-125 21.36448395328793 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.110859218392278 1101 2 1.7384763509700782 1101 3 2.0858353444477444 1101 4 2.4380313037921617 1101 5 1.9568965163558794 1101 6 1.8213700661415189 1101 7 1.7337607875125265 1101 8 2.1941973894955957 1101 9 2.7831587977011125 1101 10-11 2.7083277656830624 1101 12-13 2.427297358727195 1101 14-15 2.974679048675956 1101 16-17 2.5569231215045747 1101 18-19 2.6256311550198284 1101 20-21 2.889955339232337 1101 22-23 2.782342429352415 1101 24-25 2.6418118519160068 1101 26-27 2.3296923659293753 1101 28-29 2.2398654481916047 1101 30-31 2.3998956766684714 1101 32-33 2.6441926702813987 1101 34-35 2.9449856233387663 1101 36-37 2.926637268881489 1101 38-39 2.9267027721032335 1101 40-41 2.670596439398224 1101 42-43 2.6600969060406285 1101 44-45 2.89511099374144 1101 46-47 2.932406861394629 1101 48-49 2.945984261066183 1101 50-51 2.7583001409460124 1101 52-53 2.7720718427106448 1101 54-55 2.744366738444384 1101 56-57 3.1071563273555185 1101 58-59 3.0873667026918916 1101 60-61 3.1678285045975336 1101 62-63 2.793190454348082 1101 64-65 2.760904200200663 1101 66-67 2.8147338602805547 1101 68-69 2.6478249880480575 1101 70-71 2.668217150803393 1101 72-73 2.585352832475497 1101 74-75 2.5033143900964916 1101 76-77 2.715235528099079 1101 78-79 2.6534086437373787 1101 80-81 2.503718308277996 1101 82-83 2.4990639121219225 1101 84-85 2.3864807773421077 1101 86-87 2.3997608685602003 1101 88-89 2.5426357460846276 1101 90-91 2.5422065517307786 1101 92-93 2.5149316154790533 1101 94-95 2.530485796933501 1101 96-97 2.574424051517571 1101 98-99 2.4777911944987885 1101 100-101 2.4221791515654374 1101 102-103 2.383357747064718 1101 104-105 2.429075870603878 1101 106-107 2.472818125337021 1101 108-109 2.4813462846683407 1101 110-111 2.6340069975121096 1101 112-113 2.573734873635619 1101 114-115 2.514616606711062 1101 116-117 2.585460723025971 1101 118-119 2.5441794245165354 1101 120-121 2.5767634130343 1101 122-123 2.554667863523335 1101 124-125 2.499187783076529 1102 1 1.246589580114378 1102 2 0.9897229717173097 1102 3 1.2058177802689585 1102 4 1.7710033167980512 1102 5 1.687594938528477 1102 6 1.6530523046056338 1102 7 1.6287134401411087 1102 8 1.6682608414243205 1102 9 2.17772650084763 1102 10-11 1.8641234012872978 1102 12-13 2.235831097981656 1102 14-15 2.5714302046419526 1102 16-17 1.9787702154609974 1102 18-19 2.203227685721327 1102 20-21 2.2409326860431307 1102 22-23 2.153305837354285 1102 24-25 1.8955644275587957 1102 26-27 2.0088526900784522 1102 28-29 1.576538803884393 1102 30-31 1.5817442084695479 1102 32-33 1.5222545442719877 1102 34-35 1.9476619583145762 1102 36-37 2.1551511391405747 1102 38-39 2.222607039208672 1102 40-41 1.883376265970206 1102 42-43 1.8718029833621408 1102 44-45 2.2354508327955287 1102 46-47 2.0333106857866348 1102 48-49 2.1705844748399734 1102 50-51 2.135443820300253 1102 52-53 2.2902787659449544 1102 54-55 2.0039174452090087 1102 56-57 2.184092150803945 1102 58-59 2.203996942730331 1102 60-61 2.4670100609466665 1102 62-63 2.413655732839846 1102 64-65 2.36192746904657 1102 66-67 2.3835111046377406 1102 68-69 2.113440938592767 1102 70-71 2.3207486411802307 1102 72-73 2.2245822502685755 1102 74-75 2.348591293919082 1102 76-77 2.1199284709598487 1102 78-79 1.9778215645063568 1102 80-81 1.631205956901244 1102 82-83 1.5354328105660002 1102 84-85 1.6809518340336673 1102 86-87 1.8251904349032202 1102 88-89 1.567987190628422 1102 90-91 1.4680678405686294 1102 92-93 1.459867271110582 1102 94-95 1.7499600699104825 1102 96-97 1.8498405292090965 1102 98-99 1.9498741446016794 1102 100-101 1.8743861344297024 1102 102-103 1.628834083139619 1102 104-105 1.608363697862714 1102 106-107 1.8645856403744112 1102 108-109 1.9879495021676945 1102 110-111 1.7537970098560045 1102 112-113 1.6654736071733574 1102 114-115 1.6390422531844315 1102 116-117 1.8599952187688054 1102 118-119 1.9530997988039864 1102 120-121 1.9390547435733367 1102 122-123 2.155891819557276 1102 124-125 2.023945614316304 1103 1 1.7622031832139626 1103 2 1.514240446722475 1103 3 1.7477999246234006 1103 4 2.402489707139999 1103 5 1.813842438630175 1103 6 1.8119325162035018 1103 7 1.7672746360741947 1103 8 2.0880820359128798 1103 9 2.8304740437039158 1103 10-11 2.2876801847805375 1103 12-13 2.3923392184483205 1103 14-15 2.766927668838097 1103 16-17 2.608646791673337 1103 18-19 2.33674452480383 1103 20-21 2.7322208569685174 1103 22-23 2.5937676201460214 1103 24-25 2.645419248740833 1103 26-27 2.345036813762917 1103 28-29 2.2162365040194594 1103 30-31 2.4780099270081877 1103 32-33 2.595041724370091 1103 34-35 2.420367406577782 1103 36-37 2.791195293425705 1103 38-39 2.8840953431298892 1103 40-41 2.8955699168719136 1103 42-43 2.78385162148313 1103 44-45 2.7188004516155146 1103 46-47 2.8638322129308165 1103 48-49 2.918161327255273 1103 50-51 2.658401977253604 1103 52-53 2.763096662933787 1103 54-55 2.6690058517412467 1103 56-57 2.8282285039120367 1103 58-59 2.9937229937253527 1103 60-61 2.957530766206478 1103 62-63 2.7951453193988023 1103 64-65 2.71725429676383 1103 66-67 2.7251692688137084 1103 68-69 2.7440178391588717 1103 70-71 2.722719648586324 1103 72-73 2.7136855163042455 1103 74-75 2.7878450727192785 1103 76-77 2.8789871933530407 1103 78-79 2.989597797768763 1103 80-81 2.4824266004782167 1103 82-83 2.5855495970147047 1103 84-85 2.5242924121255506 1103 86-87 2.485742351014558 1103 88-89 2.5631768840323446 1103 90-91 2.5555236435289075 1103 92-93 2.531372249322345 1103 94-95 2.3444608747760363 1103 96-97 2.4122289625563518 1103 98-99 2.3381046150931297 1103 100-101 2.117850098950864 1103 102-103 2.120172542596233 1103 104-105 2.382374406434966 1103 106-107 2.153322088621845 1103 108-109 2.4961332497777953 1103 110-111 2.6182001599146574 1103 112-113 2.5140897697371045 1103 114-115 2.47542107629582 1103 116-117 2.5908723769417 1103 118-119 2.6619504972811754 1103 120-121 2.5519653017988624 1103 122-123 2.6334489799550695 1103 124-125 2.484945166845918 1104 1 1.5774996826407914 1104 2 1.3535628152321308 1104 3 1.3997433143177567 1104 4 1.7413123446076249 1104 5 1.267179379195614 1104 6 1.3114274773016419 1104 7 1.314329407642127 1104 8 1.5722624746273013 1104 9 2.2489984745151723 1104 10-11 2.025555567322751 1104 12-13 1.558770966104742 1104 14-15 1.9868073269802338 1104 16-17 1.6382007175486777 1104 18-19 0.9336024104218303 1104 20-21 -3.714519067406364 1104 22-23 -0.40167736467904547 1104 24-25 1.1415907567830317 1104 26-27 1.2344921164337208 1104 28-29 0.8408825014574326 1104 30-31 1.6060848415080713 1104 32-33 1.391040093193471 1104 34-35 1.6552567416613897 1104 36-37 1.8485809031047076 1104 38-39 1.823492397980683 1104 40-41 1.905253711025189 1104 42-43 1.7936857983964742 1104 44-45 1.9320260538793548 1104 46-47 1.8659619916881276 1104 48-49 1.9135676914584288 1104 50-51 1.8531453937249225 1104 52-53 1.8887740488365843 1104 54-55 1.8850517298712006 1104 56-57 2.1622863469087115 1104 58-59 2.120149500860464 1104 60-61 2.2164917605666794 1104 62-63 1.7496570398663032 1104 64-65 1.8458203071307935 1104 66-67 1.8639274197454796 1104 68-69 1.774389868638881 1104 70-71 2.012591360783073 1104 72-73 1.9577485562502446 1104 74-75 2.008906185199166 1104 76-77 2.02258644914356 1104 78-79 1.919655613830031 1104 80-81 1.698024162506897 1104 82-83 1.6450115899655522 1104 84-85 1.6178612269438268 1104 86-87 1.4720713242447268 1104 88-89 1.5893551298876574 1104 90-91 1.6513140353519518 1104 92-93 1.324358783994949 1104 94-95 1.4911306284517316 1104 96-97 1.619050770571853 1104 98-99 1.4830841521051497 1104 100-101 1.0357497669323443 1104 102-103 1.4003465374103747 1104 104-105 1.6223533849333833 1104 106-107 1.7796426564202505 1104 108-109 1.8063709428709984 1104 110-111 1.8089147721358856 1104 112-113 1.82064620456649 1104 114-115 1.7031001195184352 1104 116-117 1.7996337918111571 1104 118-119 1.8850937960289151 1104 120-121 1.8794722951022464 1104 122-123 1.654777465971236 1104 124-125 1.8374931772303142 1105 1 1.860721795033296 1105 2 1.546315930530298 1105 3 1.531178933758028 1105 4 2.2948307738036675 1105 5 1.906675937498175 1105 6 1.837755924142428 1105 7 1.6887747010579162 1105 8 1.9683356489275958 1105 9 2.4054728001882495 1105 10-11 2.1537390263373872 1105 12-13 2.317548329647501 1105 14-15 2.5125682639285323 1105 16-17 2.2984856641335583 1105 18-19 1.9752685198426043 1105 20-21 2.1739104417626507 1105 22-23 1.9016727695845113 1105 24-25 2.029233368018854 1105 26-27 2.0249755365198965 1105 28-29 1.6447366396027867 1105 30-31 2.0100211090219773 1105 32-33 2.001816838974108 1105 34-35 1.815443766462451 1105 36-37 2.0973665808896236 1105 38-39 1.9218789248721926 1105 40-41 2.0355822397110472 1105 42-43 2.2613513286552447 1105 44-45 2.4980253161525354 1105 46-47 2.7678265858218545 1105 48-49 2.3529394920828715 1105 50-51 2.477937247369276 1105 52-53 2.352869227929542 1105 54-55 2.3009091286858236 1105 56-57 2.641587071253621 1105 58-59 2.458021538571341 1105 60-61 2.8329055056698884 1105 62-63 2.7207521149311304 1105 64-65 2.278097873724615 1105 66-67 2.5221107818593964 1105 68-69 2.0907867442072217 1105 70-71 2.144268371134711 1105 72-73 2.133971829769674 1105 74-75 2.449182566080509 1105 76-77 2.3329815662440794 1105 78-79 2.077784559381193 1105 80-81 2.162764732444913 1105 82-83 2.237139171058441 1105 84-85 2.142527325873697 1105 86-87 2.144926717054229 1105 88-89 2.238689894415298 1105 90-91 2.2778596719484554 1105 92-93 2.310656241023679 1105 94-95 2.190390567202467 1105 96-97 2.3160466759957856 1105 98-99 2.0387464697445985 1105 100-101 2.224635322133846 1105 102-103 2.2044516163936727 1105 104-105 2.4050031423972804 1105 106-107 2.47962156249438 1105 108-109 2.516348281716091 1105 110-111 2.4063349345495055 1105 112-113 2.464546099873669 1105 114-115 2.291976160830817 1105 116-117 2.4573309085021258 1105 118-119 2.4738462088368856 1105 120-121 2.4610075861451683 1105 122-123 2.5333551948056936 1105 124-125 2.0469097932376528 1106 1 -0.226527420686228 1106 2 0.041343383658940525 1106 3 0.02292246079450777 1106 4 -0.2854098459633789 1106 5 -0.6520454899187875 1106 6 -0.15413987187575984 1106 7 -0.07847640581089621 1106 8 0.17675677773506493 1106 9 0.299327740757807 1106 10-11 -2.079014958986498 1106 12-13 -0.29031110132521576 1106 14-15 0.0022422493319105286 1106 16-17 -0.19453906686618794 1106 18-19 -3.002627182446112 1106 20-21 -5.383694429255009 1106 22-23 -6.251262047368133 1106 24-25 -6.165800510380578 1106 26-27 -5.242317715488497 1106 28-29 -1.7066956161839322 1106 30-31 -3.5975530349597307 1106 32-33 -3.9600529462893697 1106 34-35 -3.2779220834882707 1106 36-37 -5.764780310736594 1106 38-39 -6.221739704836139 1106 40-41 -2.3757355684237424 1106 42-43 -1.0013536921308344 1106 44-45 -0.09648880255202386 1106 46-47 0.15697542953807186 1106 48-49 0.3978263841330225 1106 50-51 0.23053318924773336 1106 52-53 0.046769782953706596 1106 54-55 0.06677685653784948 1106 56-57 0.21304220961554066 1106 58-59 0.3554593542044877 1106 60-61 0.6406878582331075 1106 62-63 0.4070935789920078 1106 64-65 0.3050051027504139 1106 66-67 -0.0751573850969649 1106 68-69 0.05559070886425843 1106 70-71 0.2775255892971593 1106 72-73 0.3046300441140559 1106 74-75 0.21234141301598797 1106 76-77 0.16435902838134808 1106 78-79 0.09286949715542647 1106 80-81 -0.19732855652534909 1106 82-83 -0.3372702256682487 1106 84-85 -0.3816830282183403 1106 86-87 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2216 112-113 -7.366192957873354 2216 114-115 -7.786382018253779 2216 116-117 -7.36630347970188 2216 118-119 -7.670799880919557 2216 120-121 -8.342939964595367 2216 122-123 -10.852090301096696 2216 124-125 -9.571985776437362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 309802.0 3 46087.0 4 26431.0 5 21555.0 6 21889.0 7 24656.0 8 26612.0 9 28562.0 10 29706.0 11 30164.0 12 30330.0 13 30475.0 14 30062.0 15 25948.0 16 21552.0 17 19202.0 18 17696.0 19 17109.0 20 17452.0 21 19179.0 22 23729.0 23 31858.0 24 42430.0 25 53719.0 26 64295.0 27 73556.0 28 83555.0 29 94769.0 30 105613.0 31 118558.0 32 139500.0 33 166422.0 34 201294.0 35 249233.0 36 316904.0 37 315126.0 38 83325.0 39 195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.801044420699746 18.640953823937224 13.964237692665302 23.593764062697723 2 15.131263076604728 20.105816527881654 40.22066073116732 24.542259664346293 3 18.911731815616474 25.143740009718492 28.91034877347156 27.034179401193477 4 11.455574692838715 16.23670906666459 37.341938459863165 34.96577778063353 5 13.86373943607432 37.961749533883534 33.74033905344114 14.434171976601004 6 32.2484769082251 36.1184347510999 17.383594685876687 14.249493654798314 7 28.669457073912213 32.36572700271661 20.76090707727544 18.203908846095736 8 25.97880731039289 35.27677960894015 20.086274236743076 18.658138843923876 9 26.180852034762857 16.32373670280192 20.326670790614976 37.16874047182025 10-11 24.653841432712834 26.905837633227975 27.260475381968945 21.17984555209024 12-13 25.428590116699144 24.13499018957802 27.81603223571649 22.62038745800634 14-15 22.64593561359367 25.383674574593556 25.490429728369318 26.479960083443455 16-17 22.72432836300262 28.930029396004244 25.594115389881622 22.75152685111152 18-19 22.626053019653664 27.4761132984575 27.77507317637179 22.12276050551705 20-21 23.373074226343867 26.709826719507102 27.681909485401494 22.235189568747536 22-23 23.12225130721904 26.648269082084937 27.420447011947612 22.80903259874841 24-25 22.814766534817572 26.74951619367263 27.559271662936453 22.87644560857334 26-27 22.825354861205128 27.08220198427849 27.118320646167426 22.974122508348955 28-29 22.677064213981254 27.166955675215238 27.36785886918223 22.788121241621287 30-31 22.70357659893709 26.7543039823477 27.565463492920863 22.97665592579435 32-33 22.738811777169175 26.91224182259339 27.13324458969136 23.215701810546072 34-35 22.983987656391143 26.734552233154652 27.36689511177944 22.914564998674766 36-37 22.995153732724347 26.65271234636234 27.276499195105565 23.07563472580775 38-39 22.869945758618755 26.793099182018608 27.388483357435355 22.948471701927282 40-41 22.962439377107653 26.66754690703428 27.400406184156317 22.969607531701755 42-43 22.901685527625975 26.731228498616254 27.143960411001068 23.2231255627567 44-45 22.93331230755747 26.763449531200774 27.145500512164887 23.157737649076868 46-47 22.974849901872567 27.024152936825946 26.805707007644003 23.195290153657485 48-49 23.14680225188102 26.97467208520748 26.61151672042615 23.267008942485358 50-51 23.0564271482591 27.192881042638966 26.55171108239982 23.198980726702107 52-53 23.4105865454468 26.954962745309487 26.41925648825905 23.21519422098466 54-55 23.03414257188377 26.920897852859145 26.5863546674969 23.45860490776019 56-57 22.964013222909184 27.190101375749766 26.66325372872484 23.18263167261621 58-59 23.165239303310784 26.81228832317217 26.655446537640255 23.36702583587679 60-61 23.121254482280253 26.72134692151811 26.93682434025889 23.220574255942747 62-63 23.100147213175156 26.956777468102405 26.674925928617665 23.26814939010477 64-65 22.917712095857286 26.761404171192755 26.76265488338038 23.558228849569577 66-67 22.903808264544388 27.14382947969307 26.502372084163568 23.44999017159898 68-69 22.844952454096088 26.923242038752676 26.74675548347209 23.48505002367915 70-71 22.63625802076992 26.79989237472929 27.006709340447056 23.557140264053732 72-73 22.887944911691562 26.891666363869128 26.68622597935287 23.53416274508644 74-75 22.81429065162079 27.031619828579107 26.58392787579548 23.57016164400462 76-77 23.032559972781478 26.775140753265536 26.764864509928827 23.427434764024156 78-79 22.93489850796121 26.9495322123378 26.749479202938776 23.36609007676221 80-81 22.899692287307357 27.114884644499476 26.61176501208491 23.37365805610826 82-83 22.95961292551338 27.051322050421224 26.529536096744284 23.459528927321113 84-85 22.93315089524181 26.954399885210023 26.538235523272096 23.574213696276068 86-87 22.961655955685693 26.804392558509 26.70929087813143 23.524660607673876 88-89 22.9669300356214 26.708241868565214 26.654072866687805 23.670755229125582 90-91 22.731793785007564 26.99838779926233 26.898738616441403 23.3710797992887 92-93 22.913242157303156 26.795406247792343 26.69748344827516 23.593868146629337 94-95 22.819591283486133 26.64462214890951 26.729076369634676 23.806710197969686 96-97 22.986607834723433 26.594030353726833 26.688250923150324 23.731110888399414 98-99 22.720912643920986 26.590455265659656 26.61462342875251 24.07400866166684 100-101 22.540617608028 26.681879608416043 26.88448597675942 23.893016806796542 102-103 22.74611961138822 26.404472354692242 27.018713003176238 23.8306950307433 104-105 22.706136101982857 26.40170994484289 27.05314728145541 23.83900667171885 106-107 22.40723170685953 26.558131800047796 27.069788445398675 23.964848047694005 108-109 22.547405954100967 26.609764255079956 26.696175835482354 24.14665395533672 110-111 22.280572183782944 26.60331790879807 27.066729987257016 24.049379920161975 112-113 22.434757389751383 26.62898011907295 27.024137902477946 23.91212458869772 114-115 22.40797639328304 26.652706795381416 26.83790324761364 24.10141356372191 116-117 22.087358499144052 26.59060913795665 27.17617537883839 24.14585698406091 118-119 22.36624997993042 26.706315706749212 26.825752947270665 24.101681366049707 120-121 22.168861507509018 26.62579853750891 27.091459269727963 24.11388068525411 122-123 22.327191455747013 26.506752054212484 27.03836794681509 24.127688543225418 124-125 22.273395810315364 26.6838500349518 26.670880497976107 24.371873656756723 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 529.0 1 535.0 2 688.5 3 801.0 4 698.0 5 597.0 6 589.0 7 643.0 8 764.0 9 932.5 10 999.5 11 1047.5 12 1178.0 13 1337.5 14 1563.5 15 1773.0 16 1945.0 17 2124.0 18 2345.0 19 2731.0 20 3318.0 21 4115.0 22 5071.0 23 6216.5 24 7670.5 25 9855.5 26 13042.5 27 16899.0 28 20528.5 29 24517.5 30 29608.5 31 34558.0 32 39614.5 33 45062.5 34 51060.0 35 56040.5 36 61696.5 37 68729.0 38 74330.5 39 78395.0 40 82889.5 41 89019.0 42 94244.0 43 98762.0 44 102674.0 45 105716.0 46 107669.0 47 109339.5 48 114244.5 49 118268.0 50 117423.5 51 114933.5 52 112553.0 53 111249.0 54 108173.5 55 100730.5 56 92343.0 57 84456.0 58 77837.5 59 70883.5 60 61187.0 61 49923.0 62 39163.0 63 30087.0 64 22521.0 65 16824.5 66 12718.5 67 9769.0 68 7398.0 69 5445.0 70 4007.0 71 3013.0 72 2421.0 73 1952.5 74 1413.5 75 1032.5 76 756.5 77 501.5 78 332.5 79 206.5 80 132.5 81 105.5 82 83.5 83 56.5 84 41.0 85 31.5 86 25.0 87 22.0 88 19.0 89 15.0 90 8.5 91 6.0 92 6.0 93 4.5 94 5.0 95 4.0 96 2.0 97 2.5 98 2.5 99 4.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10261783644014806 2 0.03305673387301212 3 0.043974244139865816 4 0.052491930168494697 5 0.05648037045174156 6 0.003177232090044109 7 0.002737827652059286 8 0.0026026262865254937 9 0.007436075104358554 10-11 0.0441770461881665 12-13 0.007317773909516487 14-15 0.012810329384326783 16-17 0.022477227019992902 18-19 0.021226614388805326 20-21 0.13927430667049737 22-23 0.018691588785046728 24-25 0.016781869496881915 26-27 0.008297983809636478 28-29 0.005408054621351676 30-31 0.00233222355545791 32-33 0.007300873738824762 34-35 0.01967179868516672 36-37 0.0029575298710516973 38-39 0.009869699683966808 40-41 0.034628449747342444 42-43 0.01539605550016055 44-45 0.11650977674874516 46-47 0.005137651890284092 48-49 0.010545706511635767 50-51 0.012303324263575062 52-53 0.01348633621199574 54-55 0.011390715046221968 56-57 0.00674316810599787 58-59 0.016004461645062616 60-61 0.03148501799868179 62-63 0.008619087052779233 64-65 0.007959980395801997 66-67 0.009683797806357844 68-69 0.008483885687245441 70-71 0.004630646769532372 72-73 9.295093880448193E-4 74-75 0.0014365145087965388 76-77 0.009142992344222675 78-79 0.019739399367933615 80-81 0.003667337040104105 82-83 0.00517145223166754 84-85 0.0034983353331868652 86-87 0.004647546940224096 88-89 0.007300873738824762 90-91 0.005796758547261327 92-93 0.0032786331141944534 94-95 0.004816548647141336 96-97 0.00224772270199929 98-99 0.003802538405637897 100-101 0.0035152355038785893 102-103 6.422064862855115E-4 104-105 0.003109631407277213 106-107 7.774078518193033E-4 108-109 0.001166111777728955 110-111 0.0020280204830068786 112-113 0.0037687380642544488 114-115 0.002366023896841358 116-117 0.004444744891923409 118-119 0.0031265315779689376 120-121 0.005847459059336499 122-123 0.003954639941863413 124-125 0.054790353382569164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2958550.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.07064702686405 #Duplication Level Percentage of deduplicated Percentage of total 1 79.19205105322742 45.98733644045367 2 10.615764944034636 12.32928677970385 3 3.998336818588124 6.965580182602365 4 1.9411461830376724 4.508944592909005 5 1.1236873904681235 3.2626626910206187 6 0.7051929106530976 2.4570605160229753 7 0.48516604763905646 1.972173441130645 8 0.3254444451315123 1.5119015600068544 9 0.24873845445236784 1.2999962691460036 >10 1.2556887538392898 12.923658654077496 >50 0.07427960052612546 2.990373782485573 >100 0.0337688884391792 3.3853195128482914 >500 4.896733090254869E-4 0.1722133803923856 >1k 2.4483665441997E-4 0.2334921972002965 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6900170691723987E-5 0.0 12-13 0.0 0.0 0.0 3.3800341383447974E-5 0.0 14-15 0.0 0.0 0.0 3.3800341383447974E-5 0.0 16-17 0.0 0.0 0.0 6.760068276689595E-5 0.0 18-19 0.0 0.0 0.0 1.352013655337919E-4 0.0 20-21 0.0 0.0 0.0 2.704027310675838E-4 1.6900170691723987E-5 22-23 0.0 0.0 0.0 6.760068276689595E-4 3.3800341383447974E-5 24-25 0.0 0.0 0.0 0.001098511094962059 3.3800341383447974E-5 26-27 0.0 0.0 0.0 0.0015548157036386068 5.0700512075171965E-5 28-29 0.0 0.0 0.0 0.002467424920991702 6.760068276689595E-5 30-31 0.0 0.0 0.0 0.005374254279968228 6.760068276689595E-5 32-33 0.0 0.0 0.0 0.012016021361815754 6.760068276689595E-5 34-35 0.0 0.0 0.0 0.020973111828429467 6.760068276689595E-5 36-37 0.0 0.0 0.0 0.035372057257778304 6.760068276689595E-5 38-39 0.0 3.3800341383447974E-5 0.0 0.06023220834530429 6.760068276689595E-5 40-41 0.0 3.3800341383447974E-5 3.3800341383447974E-5 0.08840479288840816 6.760068276689595E-5 42-43 1.6900170691723987E-5 3.3800341383447974E-5 3.3800341383447974E-5 0.11590137060384309 6.760068276689595E-5 44-45 3.3800341383447974E-5 3.3800341383447974E-5 3.3800341383447974E-5 0.14934680840276487 6.760068276689595E-5 46-47 3.3800341383447974E-5 5.0700512075171965E-5 3.3800341383447974E-5 0.19119163103547346 6.760068276689595E-5 48-49 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.23827550658261648 6.760068276689595E-5 50-51 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.28983792736306635 6.760068276689595E-5 52-53 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.34275236179885415 6.760068276689595E-5 54-55 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.3982863226918592 6.760068276689595E-5 56-57 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.4667320139933413 8.450085345861993E-5 58-59 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.5458248128306096 1.0140102415034392E-4 60-61 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.6317283804566426 1.0140102415034392E-4 62-63 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.7203528755640431 1.0140102415034392E-4 64-65 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.8149600310963141 1.0140102415034392E-4 66-67 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 0.9236619289854827 1.352013655337919E-4 68-69 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 1.0488583934697742 1.352013655337919E-4 70-71 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 1.1922901421304357 1.352013655337919E-4 72-73 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 1.3385611194673066 1.352013655337919E-4 74-75 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 1.4918118672998597 1.352013655337919E-4 76-77 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 1.6640415068192187 1.352013655337919E-4 78-79 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 1.8639198255902385 1.352013655337919E-4 80-81 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 2.0879653884504235 1.352013655337919E-4 82-83 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 2.323418566527522 1.352013655337919E-4 84-85 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 2.565936015953761 1.521015362255159E-4 86-87 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 2.8203173852055907 1.6900170691723986E-4 88-89 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 3.104037450778253 1.6900170691723986E-4 90-91 3.3800341383447974E-5 6.760068276689595E-5 3.3800341383447974E-5 3.423028172584543 1.6900170691723986E-4 92-93 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 3.7667945446249007 1.6900170691723986E-4 94-95 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 4.11852089706106 1.6900170691723986E-4 96-97 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 4.474506092511534 1.6900170691723986E-4 98-99 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 4.8572442581670074 1.6900170691723986E-4 100-101 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 5.292677156039276 1.6900170691723986E-4 102-103 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 5.753865914045732 1.6900170691723986E-4 104-105 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 6.220969731794291 1.6900170691723986E-4 106-107 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 6.6955941255006675 1.6900170691723986E-4 108-109 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 7.186189180510723 1.6900170691723986E-4 110-111 3.3800341383447974E-5 6.760068276689595E-5 6.760068276689595E-5 7.728600158861605 1.6900170691723986E-4 112-113 6.760068276689595E-5 1.0140102415034392E-4 6.760068276689595E-5 8.324466377110408 1.6900170691723986E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7600 0.0 49.03139 1 GGTATCA 4160 0.0 48.079437 1 ATCAACG 8990 0.0 40.83921 3 TCAACGC 9030 0.0 40.76143 4 CAACGCA 9125 0.0 40.270428 5 AACGCAG 9150 0.0 39.16284 6 TATCAAC 9790 0.0 38.41241 2 GTACATG 12355 0.0 35.41262 1 TACATGG 12180 0.0 34.441257 2 ACGCAGA 10925 0.0 31.983805 7 CGCAGAG 10935 0.0 30.813461 8 ACATGGG 12505 0.0 30.644606 3 GCAGAGT 11720 0.0 26.311462 9 CATGGGG 5850 0.0 24.230911 4 CATGGGA 8495 0.0 23.557247 4 CAGAGTA 11240 0.0 22.42131 10-11 GAGTACT 5115 0.0 21.980042 12-13 AGAGTAC 11110 0.0 21.933792 10-11 GAGTACA 9660 0.0 21.074749 1 AGTACAT 9060 0.0 21.016428 2 >>END_MODULE