FastQCFastQC Report
Thu 26 May 2016
SRR1364484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958550
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59960.20266684693515405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56650.19147893393723275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33440.11302834158625003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59000.062.7963031
GTATCAA101750.047.142881
ATCAACG126950.036.0551033
TATCAAC134350.035.5330962
TCAACGC128350.035.3832134
CAACGCA129250.035.0738645
AACGCAG131550.034.0988466
GTACATG123050.029.7701641
TACATGG123250.029.2070872
ACGCAGA158800.027.9108037
CGCAGAG159650.027.826388
ACATGGG123800.026.6221243
GAGTACT100100.025.01395812-13
GCAGAGT172700.024.9661039
TGGTATC30450.024.6616122
GTACTTT106150.023.98049514-15
GTGGTAT34200.023.5506081
CAGAGTA165650.023.4453210-11
AGAGTAC159850.022.95656610-11
AGTACTT104650.022.90341412-13
Produced by FastQC (version 0.11.5)