##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364484_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2958550 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.00597826638049 31.0 28.0 31.0 25.0 34.0 2 29.168468675533624 31.0 28.0 33.0 25.0 34.0 3 29.25759814774129 31.0 28.0 34.0 25.0 34.0 4 32.70556624021903 35.0 32.0 37.0 26.0 37.0 5 31.904180764225718 35.0 32.0 37.0 22.0 37.0 6 31.951839921583208 35.0 32.0 37.0 23.0 37.0 7 31.443789694275914 35.0 32.0 37.0 17.0 37.0 8 31.532652481790066 35.0 32.0 37.0 18.0 37.0 9 32.092126210474724 37.0 30.0 39.0 15.0 39.0 10-11 32.28304862179108 37.0 31.0 39.0 17.0 39.0 12-13 32.058662351489744 37.0 30.5 39.0 14.0 39.0 14-15 33.0150059995606 37.5 31.0 40.0 16.5 41.0 16-17 33.06444305487486 38.0 31.5 40.0 17.0 41.0 18-19 32.90249277517703 38.0 31.0 40.0 15.0 41.0 20-21 32.80957631272076 38.0 31.0 40.0 13.5 41.0 22-23 32.78980970407801 38.0 31.0 40.0 13.0 41.0 24-25 32.60442683071099 37.0 31.0 40.0 10.5 41.0 26-27 32.67815602237583 37.0 31.0 40.0 10.5 41.0 28-29 32.545791519494344 37.0 30.5 40.0 10.0 41.0 30-31 32.05749623971202 37.0 30.0 40.0 9.0 41.0 32-33 31.98645806222643 37.0 30.0 40.0 8.5 41.0 34-35 31.787622652988794 37.0 30.0 40.0 8.0 41.0 36-37 31.76237548799243 37.0 30.0 40.0 8.0 41.0 38-39 31.533056057866183 37.0 30.0 40.0 4.0 41.0 40-41 31.24444778692265 36.0 29.5 40.0 2.0 41.0 42-43 31.12797992259722 36.0 29.5 40.0 2.0 41.0 44-45 31.044919301684946 36.0 29.0 40.0 2.0 41.0 46-47 30.886114650757975 36.0 29.0 40.0 2.0 41.0 48-49 30.608542360277838 36.0 28.0 40.0 2.0 41.0 50-51 30.265162833144615 36.0 28.0 40.0 2.0 41.0 52-53 30.159212959050883 35.0 28.0 40.0 2.0 41.0 54-55 29.683469436041307 35.0 26.5 39.5 2.0 41.0 56-57 29.698567710533876 35.0 27.0 39.0 2.0 41.0 58-59 29.4490032279326 35.0 26.5 39.0 2.0 41.0 60-61 29.155616095722564 34.5 26.0 39.0 2.0 41.0 62-63 28.87873079718105 34.0 26.0 39.0 2.0 40.0 64-65 28.40448158726403 34.0 25.0 38.0 2.0 40.0 66-67 28.00500414054182 34.0 23.5 38.0 2.0 40.0 68-69 27.81704889219381 34.0 23.5 37.0 2.0 40.0 70-71 27.87397627216035 34.0 25.5 37.0 2.0 39.5 72-73 27.646234472968175 34.0 25.0 36.5 2.0 39.0 74-75 27.296084230450727 34.0 24.0 36.0 2.0 39.0 76-77 27.208355782393404 33.0 25.0 36.0 2.0 38.5 78-79 26.820246573490394 33.0 24.0 35.0 2.0 37.0 80-81 26.59256189687516 33.0 23.0 35.0 2.0 37.0 82-83 26.44912321914451 33.0 23.5 35.0 2.0 36.5 84-85 26.04439877642764 33.0 21.0 35.0 2.0 36.0 86-87 25.919133359246928 33.0 21.0 35.0 2.0 36.0 88-89 25.876626049923104 33.0 21.5 35.0 2.0 35.5 90-91 25.72663500701357 33.0 20.0 35.0 2.0 35.0 92-93 25.737893055719866 33.0 21.5 35.0 2.0 35.0 94-95 25.746352267157896 33.0 23.0 35.0 2.0 35.0 96-97 25.627141167125785 33.0 20.0 35.0 2.0 35.0 98-99 25.49817833060114 33.0 20.0 35.0 2.0 35.0 100-101 25.40938212975951 33.0 20.0 35.0 2.0 35.0 102-103 24.255199168511602 30.5 18.5 34.0 2.0 35.0 104-105 25.21308056311369 32.0 19.5 35.0 2.0 35.0 106-107 25.32487468523432 33.0 19.0 35.0 2.0 35.0 108-109 25.398477463622385 33.0 19.0 35.0 2.0 35.0 110-111 25.332223724459617 33.0 19.0 35.0 2.0 35.0 112-113 25.276098257592402 33.0 19.0 35.0 2.0 35.0 114-115 25.214888881377703 33.0 18.5 35.0 2.0 35.0 116-117 25.12727873451522 33.0 18.0 35.0 2.0 35.0 118-119 25.001569687853845 33.0 16.0 35.0 2.0 35.0 120-121 24.830225279275318 33.0 4.5 35.0 2.0 35.0 122-123 24.530246066485272 33.0 2.0 35.0 2.0 35.0 124-125 23.74438728431157 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8451472668123294 1101 2 0.8409508504507706 1101 3 0.7739627102083979 1101 4 0.9729136202680166 1101 5 1.0735187019427883 1101 6 1.0850398670602033 1101 7 1.449615878784055 1101 8 1.3260919895002488 1101 9 1.903923888922968 1101 10-11 1.5879240697478778 1101 12-13 1.8169078137078145 1101 14-15 1.7642560788785744 1101 16-17 1.736424407234459 1101 18-19 1.69734255849324 1101 20-21 1.9239708186189475 1101 22-23 1.979763414226774 1101 24-25 2.069846708584336 1101 26-27 1.8655384470289462 1101 28-29 1.846727072014538 1101 30-31 2.3653482841099596 1101 32-33 2.1234279707347063 1101 34-35 2.190572157236865 1101 36-37 2.0191061204800924 1101 38-39 2.081241411451245 1101 40-41 2.3313953297949794 1101 42-43 2.218797709398764 1101 44-45 2.171033702954759 1101 46-47 1.9285431003893265 1101 48-49 1.960051911879777 1101 50-51 2.286262623616622 1101 52-53 2.1818091172979486 1101 54-55 2.527256319885435 1101 56-57 2.146417859644682 1101 58-59 2.29620613894447 1101 60-61 2.4036961611232925 1101 62-63 2.4605943835450432 1101 64-65 2.5663640644898003 1101 66-67 2.802874804652113 1101 68-69 2.744859029387843 1101 70-71 2.3616867230115766 1101 72-73 2.6577274772540704 1101 74-75 2.716877469348937 1101 76-77 2.3864432604350156 1101 78-79 2.3844116158148516 1101 80-81 2.469029855165374 1101 82-83 2.421858177873741 1101 84-85 2.717058734690127 1101 86-87 2.615996060759727 1101 88-89 2.4756278460011423 1101 90-91 2.4555692773036206 1101 92-93 2.324850106453617 1101 94-95 2.338767782740952 1101 96-97 2.127640298427629 1101 98-99 2.2064123794148607 1101 100-101 2.339902745019984 1101 102-103 2.131767558673726 1101 104-105 2.203915020587754 1101 106-107 2.3019179769574585 1101 108-109 2.2776246557752415 1101 110-111 2.325700027366171 1101 112-113 2.1582235242325822 1101 114-115 2.232408222982862 1101 116-117 2.2351702522673484 1101 118-119 2.19501318332021 1101 120-121 2.122433392486709 1101 122-123 2.1829649774799798 1101 124-125 2.060018720838851 1102 1 0.5128913873872101 1102 2 -1.6004691806773756 1102 3 0.08515240198593332 1102 4 0.5204802568421982 1102 5 1.0026538351027483 1102 6 0.5823917681381374 1102 7 1.4250896775101616 1102 8 1.0971818712365007 1102 9 1.859270282145431 1102 10-11 1.6372775543414306 1102 12-13 1.6862485611138993 1102 14-15 1.6743108986540136 1102 16-17 1.6399943671737134 1102 18-19 1.820003646410136 1102 20-21 1.95106848981505 1102 22-23 1.9033885183382537 1102 24-25 2.1386068923774673 1102 26-27 1.821079806408953 1102 28-29 1.7922182622632477 1102 30-31 2.1393470535347596 1102 32-33 1.7922360184324297 1102 34-35 2.035153063865586 1102 36-37 1.4255677229944546 1102 38-39 1.3848221326693633 1102 40-41 1.6858822109799192 1102 42-43 1.5639946008163506 1102 44-45 1.5442409032181814 1102 46-47 1.2343519271724759 1102 48-49 1.2229492779460465 1102 50-51 1.408408716261473 1102 52-53 1.2884311629342164 1102 54-55 1.543149855239907 1102 56-57 1.2157639056682576 1102 58-59 1.2077421724756974 1102 60-61 1.176916111697686 1102 62-63 1.479868965900593 1102 64-65 1.6206281207643976 1102 66-67 1.6955757704071672 1102 68-69 1.6493942030420357 1102 70-71 1.1151486333953038 1102 72-73 1.4105720289813917 1102 74-75 1.5730267712663384 1102 76-77 1.5345858386856897 1102 78-79 1.3338142479564041 1102 80-81 1.4098803054186746 1102 82-83 1.0029038747996317 1102 84-85 1.056667594191829 1102 86-87 1.135006060833959 1102 88-89 1.3253017206057507 1102 90-91 1.0543782407763373 1102 92-93 1.1226183427099485 1102 94-95 1.122566431339223 1102 96-97 1.2465396960133681 1102 98-99 0.9417345630407432 1102 100-101 0.8135422665384198 1102 102-103 0.6356639085291356 1102 104-105 0.9658897013163177 1102 106-107 1.2223580870161435 1102 108-109 1.1759331746685255 1102 110-111 1.0862784280605027 1102 112-113 1.0649789498508042 1102 114-115 1.3339595696006583 1102 116-117 0.9470331965540666 1102 118-119 0.4946010148564888 1102 120-121 0.816649090264395 1102 122-123 1.2004615086694983 1102 124-125 1.1269824600453298 1103 1 0.8321819268339219 1103 2 0.7437178968097164 1103 3 0.6366533371907259 1103 4 0.6756636906419047 1103 5 1.0297491628976303 1103 6 1.0190889884255654 1103 7 1.3474629475058215 1103 8 1.1014523077235125 1103 9 1.9001525542655386 1103 10-11 1.5305967758924055 1103 12-13 1.628165224528999 1103 14-15 1.3357428536844935 1103 16-17 1.3724803017434084 1103 18-19 1.6493220053976145 1103 20-21 1.7660101401754744 1103 22-23 1.608663480170435 1103 24-25 1.9330469483652308 1103 26-27 1.4427298389107435 1103 28-29 1.6118019819790845 1103 30-31 2.069222239185155 1103 32-33 1.8844206659644094 1103 34-35 1.9784661627930618 1103 36-37 2.0047660785615626 1103 38-39 1.9651058944756485 1103 40-41 2.1571749313258763 1103 42-43 2.2107431141603513 1103 44-45 1.9948206845305165 1103 46-47 1.8218872204648164 1103 48-49 1.8592902860744012 1103 50-51 1.9983482704016566 1103 52-53 1.813770937405824 1103 54-55 2.1154101991528584 1103 56-57 1.8899732778067104 1103 58-59 2.042495200372443 1103 60-61 1.954973770622317 1103 62-63 2.0403515407065953 1103 64-65 2.213866046328242 1103 66-67 2.4224969458844683 1103 68-69 2.4425881287353874 1103 70-71 2.1841826868045082 1103 72-73 2.299647712079384 1103 74-75 2.279936079231394 1103 76-77 2.1266032659551506 1103 78-79 2.266953912677394 1103 80-81 2.248067336415339 1103 82-83 2.0669676239704806 1103 84-85 2.307172575724337 1103 86-87 2.176715924857753 1103 88-89 2.131653869833386 1103 90-91 2.1952398412591734 1103 92-93 2.025755969323729 1103 94-95 1.9245730952441384 1103 96-97 1.8058939439518085 1103 98-99 2.0347529043452504 1103 100-101 2.0062103937794014 1103 102-103 1.874156240513969 1103 104-105 1.8869744185009232 1103 106-107 1.8370608927965613 1103 108-109 1.7551595075117739 1103 110-111 1.7950311853165353 1103 112-113 1.7877184357496745 1103 114-115 1.6536746040243315 1103 116-117 1.69760706278948 1103 118-119 1.7369550203805488 1103 120-121 1.796211690042405 1103 122-123 1.9060523563643592 1103 124-125 1.6644145404339277 1104 1 0.7100994870563468 1104 2 0.7034184067603704 1104 3 0.6713668197092524 1104 4 0.6681268861794933 1104 5 0.8950078339204381 1104 6 0.9025741767238671 1104 7 1.272577125908537 1104 8 1.001698251765994 1104 9 1.7236466470928775 1104 10-11 1.1302728181936672 1104 12-13 1.1878600107054353 1104 14-15 1.263103445122681 1104 16-17 1.097833254616745 1104 18-19 1.133774254068463 1104 20-21 1.506808212550915 1104 22-23 1.4352871901426454 1104 24-25 1.621650478171233 1104 26-27 1.4042207810875524 1104 28-29 1.3384216516400755 1104 30-31 1.9824209258463377 1104 32-33 1.7982224681016596 1104 34-35 1.7661709016027842 1104 36-37 1.6530397169568722 1104 38-39 1.8166236849165927 1104 40-41 2.0026675482512886 1104 42-43 1.922675638586405 1104 44-45 1.6151980535590766 1104 46-47 1.578665991243703 1104 48-49 1.442703956903113 1104 50-51 1.734954757557027 1104 52-53 1.4981850011746083 1104 54-55 1.911106019965846 1104 56-57 1.8455227442875746 1104 58-59 1.886287915770545 1104 60-61 1.9278218295696625 1104 62-63 1.9585938825343518 1104 64-65 2.046389482113831 1104 66-67 2.0811873582259537 1104 68-69 2.1178960836328393 1104 70-71 1.9892496331769003 1104 72-73 2.0706191256892126 1104 74-75 2.1824878130398453 1104 76-77 2.0295407306519415 1104 78-79 2.083039381896164 1104 80-81 2.023045887116094 1104 82-83 2.069571505521232 1104 84-85 2.31768271254829 1104 86-87 2.2065375213510805 1104 88-89 2.0040434722905296 1104 90-91 2.046087237120151 1104 92-93 1.8362869414780647 1104 94-95 1.7507410224614972 1104 96-97 1.8059889308762536 1104 98-99 1.8596140087445114 1104 100-101 1.9491166767802284 1104 102-103 1.846348045970604 1104 104-105 1.7307538642494436 1104 106-107 1.899102525430866 1104 108-109 1.829436271998862 1104 110-111 1.8758198421824126 1104 112-113 1.867010434586696 1104 114-115 1.768386030737446 1104 116-117 1.8973085550903086 1104 118-119 1.8214878178048899 1104 120-121 1.8199079964849574 1104 122-123 1.8924881997872482 1104 124-125 1.581811942276147 1105 1 0.479325611459096 1105 2 0.5157671945843845 1105 3 0.44767939004736235 1105 4 0.6650747937125061 1105 5 0.7101126064251702 1105 6 0.9310337987934361 1105 7 1.4222927463130084 1105 8 1.1548109232011967 1105 9 1.7043992806102608 1105 10-11 1.4312965543059306 1105 12-13 1.526628680411477 1105 14-15 1.4671003877046758 1105 16-17 1.4937449010184807 1105 18-19 1.674822981757977 1105 20-21 1.8849507290082173 1105 22-23 1.8157629919100273 1105 24-25 1.9852171238854623 1105 26-27 1.6767154028972442 1105 28-29 1.7199328896813455 1105 30-31 1.9774265945386098 1105 32-33 1.9593188581333116 1105 34-35 2.086862701487263 1105 36-37 1.797152553603798 1105 38-39 1.9750338427710687 1105 40-41 2.076749191674736 1105 42-43 2.0262179446009725 1105 44-45 1.9700992320400346 1105 46-47 1.746767883074149 1105 48-49 1.8196358149536458 1105 50-51 1.9458182374926096 1105 52-53 1.8808249124039413 1105 54-55 2.0386229061179897 1105 56-57 1.94409136044996 1105 58-59 1.8257007190147476 1105 60-61 1.7577894511142027 1105 62-63 1.6123321242122834 1105 64-65 2.1214250170502673 1105 66-67 2.254590280159128 1105 68-69 2.285773341970245 1105 70-71 2.1104633876018006 1105 72-73 2.2878089214505195 1105 74-75 2.349892580251076 1105 76-77 2.1810263308442046 1105 78-79 2.3310773624947174 1105 80-81 2.3216269842517008 1105 82-83 2.1976423074355758 1105 84-85 2.4238393359889585 1105 86-87 2.388891347211832 1105 88-89 2.2391883312627385 1105 90-91 2.210449401728532 1105 92-93 2.123025088577684 1105 94-95 1.9851266522605329 1105 96-97 2.017560046356529 1105 98-99 2.0954941520006223 1105 100-101 2.0518700427389405 1105 102-103 1.9194813436455433 1105 104-105 1.5179246810832936 1105 106-107 1.7036448188723625 1105 108-109 1.6957098313874326 1105 110-111 1.8642585449075746 1105 112-113 1.994009862872435 1105 114-115 1.896364376549215 1105 116-117 1.857435329264458 1105 118-119 1.9459375758614037 1105 120-121 2.00784834920519 1105 122-123 2.1737295332789337 1105 124-125 1.8580212252532462 1106 1 0.3717607824640332 1106 2 0.3520401997034064 1106 3 0.4318251171760501 1106 4 0.3276603094299091 1106 5 0.5782644766426515 1106 6 0.6820930576748836 1106 7 0.8234252168494969 1106 8 0.5563922518942022 1106 9 1.4646198495580478 1106 10-11 1.0408707294203907 1106 12-13 0.8830196554893703 1106 14-15 1.1886955986257846 1106 16-17 1.0674432951644661 1106 18-19 1.2070838855087445 1106 20-21 1.161903622168559 1106 22-23 1.063350079169183 1106 24-25 0.8800499724530866 1106 26-27 0.9738034883917592 1106 28-29 0.9872229952942462 1106 30-31 1.4384819601696357 1106 32-33 1.14230295260732 1106 34-35 1.0820783587460951 1106 36-37 1.077940240466571 1106 38-39 1.2763919181441352 1106 40-41 1.469223775423167 1106 42-43 1.4463824646531194 1106 44-45 1.3794050444592258 1106 46-47 1.428746952588952 1106 48-49 1.2535239599316021 1106 50-51 1.4842659483739737 1106 52-53 1.1712440056591262 1106 54-55 1.5841380836674297 1106 56-57 1.4168832366555257 1106 58-59 1.4262297522963294 1106 60-61 1.6301348041199049 1106 62-63 1.6661246816952726 1106 64-65 1.6445103277551638 1106 66-67 1.841712108261273 1106 68-69 1.833800253918863 1106 70-71 1.665751559340677 1106 72-73 1.627759524697332 1106 74-75 1.8232132992984944 1106 76-77 1.4042300122594042 1106 78-79 1.4391113356172127 1106 80-81 1.5093686771052077 1106 82-83 1.3761140153785334 1106 84-85 1.718494885170763 1106 86-87 1.7149501589739202 1106 88-89 1.4093155913682978 1106 90-91 1.5453699949863697 1106 92-93 1.503940353497562 1106 94-95 1.460618715395114 1106 96-97 1.3511351829432137 1106 98-99 1.3458289947205877 1106 100-101 0.9900003982352601 1106 102-103 0.8711111019891185 1106 104-105 1.1394363634841476 1106 106-107 1.1155776645254356 1106 108-109 1.1272148715818062 1106 110-111 1.0013168158208359 1106 112-113 0.8786249576882383 1106 114-115 0.957953950454403 1106 116-117 1.3190751378576273 1106 118-119 1.0576099827678398 1106 120-121 1.0652102025712367 1106 122-123 1.2205832951093818 1106 124-125 1.239472122605239 1107 1 0.44379699237885717 1107 2 0.4866197010670845 1107 3 0.340445366277752 1107 4 0.499771044897507 1107 5 0.3848436523058254 1107 6 0.8006508757723267 1107 7 0.9628655362051042 1107 8 0.9084327757342621 1107 9 1.6964549859125881 1107 10-11 1.0934519355539791 1107 12-13 1.4647156504215673 1107 14-15 1.2744561067794535 1107 16-17 1.2565230736948791 1107 18-19 1.4556892159713115 1107 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-5.418845928269459 2216 112-113 -5.5220636286265545 2216 114-115 -5.37127392108016 2216 116-117 -5.379735132300375 2216 118-119 -5.449883972668012 2216 120-121 -5.313203732162378 2216 122-123 -5.462296231162174 2216 124-125 -5.462135936989906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 135130.0 3 45105.0 4 22024.0 5 18509.0 6 18814.0 7 22823.0 8 25400.0 9 27168.0 10 28852.0 11 31256.0 12 33798.0 13 36258.0 14 36916.0 15 29550.0 16 22083.0 17 19110.0 18 17066.0 19 16279.0 20 15650.0 21 17046.0 22 21260.0 23 28444.0 24 39351.0 25 52240.0 26 63842.0 27 73444.0 28 82980.0 29 93817.0 30 104937.0 31 118875.0 32 138290.0 33 163822.0 34 197647.0 35 246863.0 36 345214.0 37 430680.0 38 137523.0 39 484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.50779001720588 20.091257145854094 14.079485413913922 24.321467423026103 2 16.082358190188405 20.889120985350882 38.02801381762626 25.000507006834454 3 19.251829443477376 25.170979026888173 29.387774416521605 26.189417113112846 4 13.385896629280447 17.279111236928312 35.73405645113774 33.60093568265351 5 13.544134795761437 38.55246657991246 33.6740971083808 14.229301515945311 6 32.314363986351395 36.895162993971695 16.705103353168536 14.085369666508369 7 28.040081797378374 32.66441300102247 20.651133099265877 18.644372102333282 8 26.23386456203208 36.13222693549205 19.036690270571732 18.59721823190414 9 25.87784556624022 16.04346723901911 19.707255243277956 38.37143195146271 10-11 25.19575586811297 26.95967121531895 26.691649777660004 21.15292313890808 12-13 25.88138684250153 24.011744332115896 27.519292919955106 22.58757590542747 14-15 22.86085608176337 25.187089905062894 25.536384108702357 26.415669904471383 16-17 22.65415760291356 28.660326729462117 25.951045508251557 22.734470159372762 18-19 22.526707273812278 26.935665768297934 28.450667996615188 22.0869589612746 20-21 23.202651107678772 26.174837833786242 28.428728918730222 22.19378213980476 22-23 22.899333532194866 25.946359777691963 28.44348036974725 22.71082632036593 24-25 22.624376421468355 26.232778652585313 28.447670781970903 22.695174143975425 26-27 22.614669712188086 26.523922638347713 28.23487358635498 22.626534063109222 28-29 22.443639335690072 26.486288429300497 28.593916075158482 22.476156159850948 30-31 22.508678296316077 26.14741104535023 28.45414783693253 22.88976282140117 32-33 22.527693065600605 26.238737082977377 28.201541465871344 23.032028385550678 34-35 22.789555054855093 26.248765430846888 28.17707625631988 22.78460325797814 36-37 22.831545586076274 26.12522111996258 28.125681823520793 22.917551470440355 38-39 22.7299829138408 26.314223086764226 28.259391894975046 22.696402104419924 40-41 22.778355677050545 26.09955171729036 28.280461887519486 22.841630718139605 42-43 22.89780064501704 26.207471861414795 27.84820416514775 23.046523328420417 44-45 22.89459123187306 26.328007568672213 27.729641701518094 23.04775949793663 46-47 22.98997548119921 26.564687518801467 27.332125307499023 23.1132116925003 48-49 23.150327018302885 26.703604806408542 26.93185851177097 23.2142096635176 50-51 22.996471497238232 26.786289729470187 26.884215402666918 23.333023370624666 52-53 23.352709395404332 26.724634301122425 26.64091078476854 23.281745518704703 54-55 22.97280442183059 26.594447495415785 26.77908532349275 23.653662759260875 56-57 22.991773315446153 26.936181086433425 26.827343729666936 23.244701868453493 58-59 23.115799969579694 26.57572121478427 26.903026820570886 23.40545199506515 60-61 23.124030352706562 26.55493400483345 27.020077402781766 23.30095823967822 62-63 23.051174864565457 26.67937567020136 26.981924315517915 23.28752514971527 64-65 22.84986659501683 26.50106603931351 26.977735850524194 23.671331515145468 66-67 22.77575678671156 26.86708435380936 26.768712860238253 23.588445999240825 68-69 22.74814098860079 26.67753818707454 26.99814043933494 23.57618038498973 70-71 22.715032092525984 26.68116061067908 27.00453645575071 23.599270841044227 72-73 22.902942668696095 26.725959749704042 26.70295958058515 23.668138001014714 74-75 22.802124207778363 26.893155985882107 26.551431048175626 23.753288758163905 76-77 23.03331193727609 26.630976150325385 26.747589732461403 23.588122179937123 78-79 22.95212266586356 26.85917583133528 26.66571686375077 23.52298463905039 80-81 22.785605291519094 26.96524356309545 26.7058255483987 23.543325596986765 82-83 22.885722054976 26.849561718756732 26.631616786018668 23.633099440248596 84-85 22.741228429674745 26.768658087349902 26.542956269617935 23.94715721335742 86-87 22.89430837700069 26.623764889518174 26.73802729115466 23.743899442326473 88-89 22.894154267260006 26.574606765537435 26.619190041198127 23.912048926004438 90-91 22.650166179996276 26.871945916548672 26.81424851928604 23.66363938416901 92-93 22.79831845287389 26.734327857747903 26.679096531783703 23.78825715759451 94-95 22.70052010888248 26.605883074709837 26.699260652395758 23.994336164011926 96-97 22.88011516769572 26.540336664054394 26.644545987222777 23.93500218102711 98-99 22.5039799901979 26.464754694022407 26.600479964847644 24.430785350932045 100-101 22.424696173587087 26.60610137180308 26.809226926346497 24.159975528263335 102-103 22.654876050824218 26.375869482993302 26.8093307748391 24.159923691343383 104-105 22.678338175524544 26.29516855168768 26.910572719312732 24.115920553475046 106-107 22.26233949897044 26.497271634599134 26.834244365817284 24.406144500613138 108-109 22.52549771433603 26.57080775562484 26.425449362939506 24.478245167099622 110-111 22.1917492903185 26.55326747552133 26.75710074426775 24.497882489892415 112-113 22.360623400187052 26.635421620530874 26.655549730628085 24.348405248653986 114-115 22.364350996122926 26.67155195010521 26.47946431785484 24.484632735917025 116-117 22.124807760558383 26.580858866674557 26.677240540129453 24.61709283263761 118-119 22.27158427399054 26.681382330488617 26.41792338784979 24.629110007671052 120-121 22.109425688619485 26.630000234918448 26.65423570635329 24.606338370108773 122-123 22.22216025443747 26.606031752630916 26.465438115899925 24.70636987703169 124-125 22.089263247277326 26.723503248142688 26.276423061035608 24.910810443544378 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 157.0 1 141.0 2 212.5 3 303.5 4 250.0 5 238.5 6 456.0 7 980.5 8 1567.0 9 1886.0 10 1701.0 11 1370.0 12 1375.0 13 1529.0 14 1801.0 15 2099.0 16 2390.0 17 2701.0 18 3023.5 19 3431.5 20 3986.0 21 4648.0 22 5520.5 23 6687.5 24 8006.5 25 9916.0 26 13079.5 27 16757.0 28 20146.5 29 24073.0 30 29164.5 31 33969.5 32 38800.5 33 44378.5 34 50142.0 35 54862.0 36 60535.5 37 68122.0 38 73705.5 39 77288.5 40 81920.0 41 88033.5 42 93091.5 43 97507.5 44 101731.5 45 105148.0 46 107259.0 47 109355.5 48 115242.0 49 119732.0 50 118931.5 51 115839.5 52 113081.5 53 112556.0 54 110229.5 55 102106.0 56 92812.5 57 84825.5 58 78429.5 59 72024.0 60 62226.5 61 50527.0 62 39186.0 63 29462.0 64 21864.0 65 16379.0 66 12160.0 67 9380.0 68 7036.0 69 4869.5 70 3571.5 71 2733.0 72 2116.0 73 1785.0 74 1349.0 75 954.0 76 671.5 77 397.0 78 230.0 79 142.5 80 92.0 81 65.5 82 51.5 83 33.5 84 22.5 85 21.0 86 15.0 87 6.5 88 5.5 89 3.5 90 3.5 91 2.5 92 2.5 93 2.5 94 1.0 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10697808047861283 2 3.3800341383447973E-4 3 0.0 4 0.18292744756722043 5 0.0 6 1.6900170691723986E-4 7 0.03373274070068107 8 0.0 9 0.0 10-11 6.084061449020635E-4 12-13 5.577056328268915E-4 14-15 4.225042672930997E-4 16-17 0.003667337040104105 18-19 0.001335113484646195 20-21 0.002991330212435146 22-23 0.0014365145087965388 24-25 0.004106741478088928 26-27 0.003633536698720657 28-29 0.00275472782275101 30-31 6.760068276689595E-4 32-33 8.450085345861993E-5 34-35 9.464095587365432E-4 36-37 0.0010647107535786111 38-39 5.070051207517196E-4 40-41 0.001267512801879299 42-43 7.943080225110273E-4 44-45 0.0013182133139544709 46-47 1.352013655337919E-4 48-49 0.0 50-51 0.0407294113670548 52-53 8.450085345861993E-5 54-55 0.13917290564634704 56-57 2.366023896841358E-4 58-59 0.0 60-61 0.0 62-63 5.915059742103396E-4 64-65 0.05328623819100573 66-67 0.047066975376451305 68-69 0.0018759189467813626 70-71 0.002467424920991702 72-73 0.06929069983606835 74-75 0.005965760254178568 76-77 0.002061820824390326 78-79 0.001402714167413091 80-81 1.5210153622551587E-4 82-83 1.5210153622551587E-4 84-85 1.6900170691723987E-5 86-87 3.0420307245103173E-4 88-89 0.001402714167413091 90-91 3.718037552179277E-4 92-93 0.0028392286762096296 94-95 0.004427844721231685 96-97 0.00275472782275101 98-99 0.0 100-101 0.001183011948420679 102-103 0.0013182133139544709 104-105 2.1970221899241183E-4 106-107 6.760068276689595E-5 108-109 1.6900170691723987E-5 110-111 1.5210153622551587E-4 112-113 3.3800341383447974E-5 114-115 1.5210153622551587E-4 116-117 0.0 118-119 8.281083638944752E-4 120-121 0.00258572611583377 122-123 7.943080225110273E-4 124-125 0.008247283297561305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2958550.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.59803172677194 #Duplication Level Percentage of deduplicated Percentage of total 1 76.42661102107311 39.434627002349885 2 11.273742423801645 11.634058385255443 3 4.552088297534722 7.046363891977915 4 2.395904408421975 4.944958067202793 5 1.369196020404906 3.5323909850511113 6 0.8620723503851634 2.6688741891567913 7 0.5873647896808881 2.1214806937200232 8 0.4216429411369338 1.7404756683322353 9 0.30385911229265633 1.4110678904890714 >10 1.635999589356854 15.132702005064177 >50 0.11223249963279744 3.9796016624495403 >100 0.057213646477567215 5.1596152947015925 >500 0.0017385611233598946 0.570739286133373 >1k 2.0060320651784128E-4 0.2207120791343842 >5k 1.337354710118942E-4 0.40233289898171865 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5996 0.20266684693515405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5665 0.19147893393723275 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3344 0.11302834158625003 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.3800341383447974E-5 0.0 6 0.0 0.0 0.0 3.3800341383447974E-5 0.0 7 0.0 0.0 0.0 6.760068276689595E-5 0.0 8 0.0 0.0 0.0 6.760068276689595E-5 0.0 9 0.0 0.0 0.0 6.760068276689595E-5 0.0 10-11 0.0 0.0 0.0 1.0140102415034392E-4 0.0 12-13 0.0 0.0 0.0 1.352013655337919E-4 0.0 14-15 0.0 0.0 0.0 1.352013655337919E-4 0.0 16-17 0.0 0.0 0.0 1.6900170691723986E-4 0.0 18-19 0.0 0.0 0.0 2.1970221899241183E-4 0.0 20-21 0.0 0.0 0.0 3.7180375521792767E-4 0.0 22-23 0.0 0.0 0.0 7.943080225110274E-4 0.0 24-25 3.3800341383447974E-5 0.0 0.0 0.0012337124604958512 0.0 26-27 3.3800341383447974E-5 0.0 0.0 0.0017069172398641226 0.0 28-29 3.3800341383447974E-5 0.0 0.0 0.002704027310675838 0.0 30-31 3.3800341383447974E-5 0.0 0.0 0.005543255986885468 0.0 32-33 3.3800341383447974E-5 0.0 0.0 0.012049821703199202 0.0 34-35 6.760068276689595E-5 0.0 0.0 0.02090551114566257 0.0 36-37 6.760068276689595E-5 0.0 0.0 0.03587906237853002 0.0 38-39 1.0140102415034392E-4 0.0 0.0 0.06155042165925876 0.0 40-41 1.0140102415034392E-4 1.6900170691723987E-5 0.0 0.09033141234726472 0.0 42-43 1.183011948420679E-4 3.3800341383447974E-5 0.0 0.11813219313515066 0.0 44-45 1.352013655337919E-4 3.3800341383447974E-5 0.0 0.15213533656689932 0.0 46-47 1.352013655337919E-4 3.3800341383447974E-5 0.0 0.19507867029457 0.0 48-49 1.352013655337919E-4 3.3800341383447974E-5 0.0 0.24305825488837438 0.0 50-51 1.352013655337919E-4 3.3800341383447974E-5 0.0 0.29492487874127526 0.0 52-53 1.352013655337919E-4 3.3800341383447974E-5 0.0 0.3478731135184465 0.0 54-55 1.352013655337919E-4 3.3800341383447974E-5 0.0 0.40404928089773706 0.0 56-57 1.352013655337919E-4 3.3800341383447974E-5 0.0 0.4735258826114144 0.0 58-59 1.352013655337919E-4 3.3800341383447974E-5 3.3800341383447974E-5 0.5516891720606378 0.0 60-61 1.352013655337919E-4 3.3800341383447974E-5 3.3800341383447974E-5 0.6380997448074226 0.0 62-63 1.6900170691723986E-4 3.3800341383447974E-5 3.3800341383447974E-5 0.7241216136282976 0.0 64-65 1.6900170691723986E-4 3.3800341383447974E-5 3.3800341383447974E-5 0.8160923425326596 0.0 66-67 1.6900170691723986E-4 3.3800341383447974E-5 3.3800341383447974E-5 0.923154923864731 0.0 68-69 1.6900170691723986E-4 3.3800341383447974E-5 3.3800341383447974E-5 1.0468810734988425 0.0 70-71 1.6900170691723986E-4 3.3800341383447974E-5 3.3800341383447974E-5 1.187862297409204 0.0 72-73 1.6900170691723986E-4 3.3800341383447974E-5 3.3800341383447974E-5 1.3303983370232038 0.0 74-75 1.6900170691723986E-4 5.0700512075171965E-5 3.3800341383447974E-5 1.4781058288688715 0.0 76-77 1.6900170691723986E-4 6.760068276689595E-5 3.3800341383447974E-5 1.644369708134052 0.0 78-79 1.6900170691723986E-4 6.760068276689595E-5 3.3800341383447974E-5 1.8367443511179462 0.0 80-81 2.0280204830068783E-4 6.760068276689595E-5 3.3800341383447974E-5 2.0521201264132767 0.0 82-83 2.1970221899241183E-4 6.760068276689595E-5 3.3800341383447974E-5 2.2798499264842573 0.0 84-85 2.366023896841358E-4 6.760068276689595E-5 3.3800341383447974E-5 2.517195923678829 0.0 86-87 2.366023896841358E-4 6.760068276689595E-5 3.3800341383447974E-5 2.7647834243125855 0.0 88-89 2.366023896841358E-4 6.760068276689595E-5 3.3800341383447974E-5 3.0409998140981225 0.0 90-91 2.366023896841358E-4 6.760068276689595E-5 3.3800341383447974E-5 3.356221797840158 0.0 92-93 2.366023896841358E-4 8.450085345861993E-5 3.3800341383447974E-5 3.6949012185023067 0.0 94-95 2.366023896841358E-4 1.0140102415034392E-4 3.3800341383447974E-5 4.0425884301431445 0.0 96-97 2.366023896841358E-4 1.0140102415034392E-4 3.3800341383447974E-5 4.395176691284582 0.0 98-99 2.366023896841358E-4 1.0140102415034392E-4 3.3800341383447974E-5 4.781869496881918 0.0 100-101 2.366023896841358E-4 1.0140102415034392E-4 3.3800341383447974E-5 5.224569468151628 0.0 102-103 2.366023896841358E-4 1.0140102415034392E-4 3.3800341383447974E-5 5.694816717648848 0.0 104-105 2.366023896841358E-4 1.0140102415034392E-4 3.3800341383447974E-5 6.170725524327796 0.0 106-107 2.366023896841358E-4 1.0140102415034392E-4 3.3800341383447974E-5 6.654002805428335 0.0 108-109 2.535025603758598E-4 1.0140102415034392E-4 3.3800341383447974E-5 7.155312568656944 0.0 110-111 2.704027310675838E-4 1.0140102415034392E-4 3.3800341383447974E-5 7.709283263760964 0.0 112-113 2.704027310675838E-4 1.0140102415034392E-4 3.3800341383447974E-5 8.314174173159149 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5900 0.0 62.796303 1 GTATCAA 10175 0.0 47.14288 1 ATCAACG 12695 0.0 36.055103 3 TATCAAC 13435 0.0 35.533096 2 TCAACGC 12835 0.0 35.383213 4 CAACGCA 12925 0.0 35.073864 5 AACGCAG 13155 0.0 34.098846 6 GTACATG 12305 0.0 29.770164 1 TACATGG 12325 0.0 29.207087 2 ACGCAGA 15880 0.0 27.910803 7 CGCAGAG 15965 0.0 27.82638 8 ACATGGG 12380 0.0 26.622124 3 GAGTACT 10010 0.0 25.013958 12-13 GCAGAGT 17270 0.0 24.966103 9 TGGTATC 3045 0.0 24.661612 2 GTACTTT 10615 0.0 23.980495 14-15 GTGGTAT 3420 0.0 23.550608 1 CAGAGTA 16565 0.0 23.44532 10-11 AGAGTAC 15985 0.0 22.956566 10-11 AGTACTT 10465 0.0 22.903414 12-13 >>END_MODULE