FastQCFastQC Report
Thu 26 May 2016
SRR1364483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524063
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27760.10998140696171213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.052.8168221
GTATCAA78450.049.7008131
TCAACGC94350.040.3899964
ATCAACG95100.040.165613
CAACGCA97250.039.428915
GTACATG119800.039.303821
AACGCAG95700.038.992826
TATCAAC104700.037.278382
TACATGG127100.035.5768852
ACGCAGA113400.032.6973577
ACATGGG128200.032.162153
CGCAGAG112000.032.0477268
CATGGGA82850.028.1727544
GCAGAGT117050.027.3086879
GAGTACT44600.025.27507812-13
ACGCATA4000.023.809928
AGAGTAC107100.023.66902210-11
ATGGGAT38050.023.6287545
CAGAGTA113100.023.49194710-11
CATGGGG49450.023.119094
Produced by FastQC (version 0.11.5)