##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364483_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2524063 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.24553784909489 30.0 25.0 31.0 2.0 34.0 2 25.441846340602435 30.0 25.0 31.0 2.0 34.0 3 25.453700640594153 30.0 25.0 31.0 2.0 34.0 4 28.13555129170706 35.0 26.0 35.0 2.0 37.0 5 28.73722010900679 35.0 27.0 35.0 2.0 37.0 6 28.931636809382333 35.0 28.0 37.0 2.0 37.0 7 28.639219781756637 35.0 27.0 36.0 2.0 37.0 8 28.302341898756094 35.0 26.0 36.0 2.0 37.0 9 28.788115431350167 35.0 25.0 39.0 2.0 39.0 10-11 28.905090126514274 35.0 26.0 39.0 2.0 39.0 12-13 28.746648954483305 35.0 25.5 39.0 2.0 39.0 14-15 29.29436666200487 35.5 25.0 39.5 2.0 41.0 16-17 29.5487709696628 36.0 26.5 39.5 2.0 41.0 18-19 29.50917013561072 36.0 26.0 40.0 2.0 41.0 20-21 29.318985500758103 35.5 25.5 40.0 2.0 41.0 22-23 29.374286418365944 35.0 25.0 40.0 2.0 41.0 24-25 29.412411457241756 35.5 25.5 40.0 2.0 41.0 26-27 29.524807621679805 36.0 26.5 40.0 2.0 41.0 28-29 29.461798893292283 35.0 26.0 40.0 2.0 41.0 30-31 29.10969694496532 35.0 25.0 40.0 2.0 41.0 32-33 29.117424961262852 35.0 25.0 40.0 2.0 41.0 34-35 28.8136575830318 35.0 24.5 39.5 2.0 41.0 36-37 28.56213949493337 35.0 24.0 39.0 2.0 41.0 38-39 28.402765303401697 34.0 24.0 39.0 2.0 41.0 40-41 28.45156360994159 35.0 24.0 39.0 2.0 41.0 42-43 28.302737293007347 34.0 24.0 39.0 2.0 41.0 44-45 27.93640055735534 34.0 22.5 39.0 2.0 40.5 46-47 27.675712927926128 33.5 21.0 39.0 2.0 40.0 48-49 27.42624470942286 33.0 19.0 39.0 2.0 40.0 50-51 27.423865212556105 33.0 19.5 39.0 2.0 40.0 52-53 27.26101864335399 33.0 18.5 39.0 2.0 40.0 54-55 27.134720092168855 33.0 18.0 39.0 2.0 40.0 56-57 26.739889218296057 33.0 14.5 38.5 2.0 40.0 58-59 26.50024642808044 33.0 13.5 38.0 2.0 40.0 60-61 26.110595694323003 32.5 11.5 38.0 2.0 40.0 62-63 26.0466834623383 32.0 12.0 38.0 2.0 40.0 64-65 25.86664694977899 32.0 9.5 37.5 2.0 40.0 66-67 25.649751610795768 32.0 8.0 37.0 2.0 40.0 68-69 25.48270348244081 32.0 8.0 37.0 2.0 40.0 70-71 25.343568286528505 32.0 7.5 36.0 2.0 39.0 72-73 25.311999938194887 32.0 7.0 36.0 2.0 39.0 74-75 25.016250386777195 32.0 4.5 36.0 2.0 39.0 76-77 24.726461463125126 32.0 2.0 35.5 2.0 39.0 78-79 24.422361288129494 31.0 2.0 35.0 2.0 37.5 80-81 24.409943016477797 32.0 2.0 35.0 2.0 37.0 82-83 24.191456790103892 32.0 2.0 35.0 2.0 36.5 84-85 23.972778809403728 31.0 2.0 35.0 2.0 36.0 86-87 23.755036423417323 31.0 2.0 35.0 2.0 36.0 88-89 23.501948049632674 31.0 2.0 35.0 2.0 35.5 90-91 23.31723059210487 31.0 2.0 35.0 2.0 35.0 92-93 23.19601551149872 31.0 2.0 35.0 2.0 35.0 94-95 23.12147280000539 31.0 2.0 34.0 2.0 35.0 96-97 22.986942283136358 31.0 2.0 34.0 2.0 35.0 98-99 22.879062646217626 31.0 2.0 34.0 2.0 35.0 100-101 22.795803234705314 31.0 2.0 34.0 2.0 35.0 102-103 21.726074190699677 29.0 2.0 33.5 2.0 34.5 104-105 22.46304727734609 30.0 2.0 34.0 2.0 35.0 106-107 22.518241422658626 30.0 2.0 34.0 2.0 35.0 108-109 22.47072517603562 30.5 2.0 34.0 2.0 35.0 110-111 22.34599592007014 30.0 2.0 34.0 2.0 35.0 112-113 22.313338454705764 30.5 2.0 34.0 2.0 35.0 114-115 22.253610943942366 31.0 2.0 34.0 2.0 35.0 116-117 22.054276973276817 30.0 2.0 34.0 2.0 35.0 118-119 21.892493174694927 30.0 2.0 34.0 2.0 35.0 120-121 21.71151136084955 30.0 2.0 34.0 2.0 35.0 122-123 21.436436808431488 30.0 2.0 34.0 2.0 35.0 124-125 20.458648219160935 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.2172242128511854 1101 2 1.9216246348538135 1101 3 2.164810955455483 1101 4 2.6553333913983757 1101 5 2.114798828233859 1101 6 2.0638973972496366 1101 7 1.9134254164122488 1101 8 2.2298315065489547 1101 9 2.6869156801938026 1101 10-11 2.7285618510203236 1101 12-13 2.5163304744400996 1101 14-15 2.895760717561288 1101 16-17 2.615759826008887 1101 18-19 2.5525994498827558 1101 20-21 2.936281171508899 1101 22-23 2.8800551870152873 1101 24-25 2.669201642548007 1101 26-27 2.372735812296039 1101 28-29 2.277690743513105 1101 30-31 2.49548729751654 1101 32-33 2.531266694715903 1101 34-35 2.933893609907166 1101 36-37 2.8968887034765594 1101 38-39 2.8464997590749768 1101 40-41 2.5358442836611026 1101 42-43 2.5964370571283837 1101 44-45 2.7914919300546686 1101 46-47 2.6565625189405964 1101 48-49 2.6279681111109987 1101 50-51 2.5793576212430587 1101 52-53 2.5673567145411553 1101 54-55 2.5310290241038196 1101 56-57 2.838083048521483 1101 58-59 2.862001675066921 1101 60-61 2.9447551163484995 1101 62-63 2.833116859990291 1101 64-65 2.75934377112889 1101 66-67 2.719202237180575 1101 68-69 2.5252326767900755 1101 70-71 2.5793380479724313 1101 72-73 2.36984631794013 1101 74-75 2.523950460758474 1101 76-77 2.700133285230539 1101 78-79 2.5793816071116815 1101 80-81 2.4857471032954095 1101 82-83 2.4530952519558937 1101 84-85 2.331644147736821 1101 86-87 2.2918820146009864 1101 88-89 2.4447312201066076 1101 90-91 2.422097397754669 1101 92-93 2.3990839879837402 1101 94-95 2.318200063443477 1101 96-97 2.2941346011513737 1101 98-99 2.218960053817412 1101 100-101 2.3490660403170622 1101 102-103 2.3379269849264865 1101 104-105 2.2831286271716387 1101 106-107 2.4250439411504843 1101 108-109 2.4081199496265846 1101 110-111 2.634512937609035 1101 112-113 2.5854597129743517 1101 114-115 2.4171298002782677 1101 116-117 2.480405339127028 1101 118-119 2.555884807562162 1101 120-121 2.510085846668048 1101 122-123 2.4582382384673593 1101 124-125 2.4417588369265246 1102 1 1.2696243208219329 1102 2 1.0540024615435222 1102 3 1.0977625122020669 1102 4 1.5308489554162357 1102 5 1.4789091815302058 1102 6 1.338216860134569 1102 7 1.3287666460916938 1102 8 1.4828352434106478 1102 9 1.978397295489394 1102 10-11 1.4893527995546805 1102 12-13 1.9435841360005668 1102 14-15 2.3091654189682345 1102 16-17 1.7445491153303472 1102 18-19 2.0314856764454845 1102 20-21 1.9352049119958856 1102 22-23 2.0053558069850936 1102 24-25 1.6705437344664773 1102 26-27 1.7016729210590817 1102 28-29 1.4976569767944454 1102 30-31 1.3650296520226313 1102 32-33 1.2935502760445488 1102 34-35 1.73512457864663 1102 36-37 2.0563539748971422 1102 38-39 2.08153962870227 1102 40-41 1.6095104915013465 1102 42-43 1.8102733521456464 1102 44-45 2.0921901564922827 1102 46-47 1.989645166312414 1102 48-49 1.998381078289519 1102 50-51 1.9917419387381372 1102 52-53 2.006203809096096 1102 54-55 1.6804522647581592 1102 56-57 1.8895480482912106 1102 58-59 1.9178245407297823 1102 60-61 2.376120113877043 1102 62-63 2.2235802709842467 1102 64-65 2.333487830804401 1102 66-67 2.2365108360015142 1102 68-69 2.1326076034899053 1102 70-71 2.3789634012970993 1102 72-73 2.2027392961226013 1102 74-75 2.238320791066709 1102 76-77 2.18449593641483 1102 78-79 2.035974546307447 1102 80-81 1.577216324366745 1102 82-83 1.5577194674501378 1102 84-85 1.643967362260458 1102 86-87 1.7247563306405311 1102 88-89 1.403781731403761 1102 90-91 1.327087325985154 1102 92-93 1.3484980053157045 1102 94-95 1.604070598536552 1102 96-97 1.6379970835082283 1102 98-99 1.6693090562085793 1102 100-101 1.5847568376086336 1102 102-103 1.3868221996107053 1102 104-105 1.4610494403312622 1102 106-107 1.7817026442356045 1102 108-109 1.8908015610308944 1102 110-111 1.6994337056987554 1102 112-113 1.5655034726517592 1102 114-115 1.7197695422321182 1102 116-117 1.8919988090640452 1102 118-119 1.899212331064394 1102 120-121 1.9326616890997173 1102 122-123 2.0909522423276066 1102 124-125 2.0135970044383136 1103 1 1.7224984809136394 1103 2 1.4414851983509749 1103 3 1.6216750325654345 1103 4 2.2375459300117555 1103 5 1.7959139890202351 1103 6 1.8486688882044646 1103 7 1.7427972389416482 1103 8 1.9931171307693774 1103 9 2.6664832383235932 1103 10-11 2.1796490030916864 1103 12-13 2.377507721046193 1103 14-15 2.5699376142115256 1103 16-17 2.4889687952591295 1103 18-19 2.130939380825815 1103 20-21 2.687244570651547 1103 22-23 2.4252704635186078 1103 24-25 2.4002037077016105 1103 26-27 2.1891148019517246 1103 28-29 1.9737877297464443 1103 30-31 2.167327345121265 1103 32-33 2.218531508896689 1103 34-35 2.130572096695939 1103 36-37 2.322051946716524 1103 38-39 2.5744278576961612 1103 40-41 2.5879537372103663 1103 42-43 2.534727056525977 1103 44-45 2.474564796742481 1103 46-47 2.6125178538196607 1103 48-49 2.565126891757725 1103 50-51 2.5367283791508726 1103 52-53 2.5823428845492558 1103 54-55 2.477259598302595 1103 56-57 2.615704820592743 1103 58-59 2.7333675590186175 1103 60-61 2.691384392963908 1103 62-63 2.524633833199278 1103 64-65 2.4039050483062354 1103 66-67 2.512234838242499 1103 68-69 2.387525076018271 1103 70-71 2.434227023441519 1103 72-73 2.43508655146875 1103 74-75 2.4497931467072966 1103 76-77 2.512666551010323 1103 78-79 2.667017032768161 1103 80-81 2.1522855616713343 1103 82-83 2.212566613920991 1103 84-85 2.212126524651463 1103 86-87 2.274729074910553 1103 88-89 2.253873278916572 1103 90-91 2.2289010038220525 1103 92-93 2.1519650006012228 1103 94-95 2.032137246454525 1103 96-97 2.124920330486848 1103 98-99 2.0361701667660874 1103 100-101 1.854258432354868 1103 102-103 1.9331153280665738 1103 104-105 2.1348475309169856 1103 106-107 1.9680937309291267 1103 108-109 2.2442614148353677 1103 110-111 2.445693470336206 1103 112-113 2.1885366128144845 1103 114-115 2.1775620975241594 1103 116-117 2.3475487178671486 1103 118-119 2.3855730249432447 1103 120-121 2.2844721744916043 1103 122-123 2.3098152004434667 1103 124-125 2.2361190950506042 1104 1 1.7800175611607187 1104 2 1.5516490458102652 1104 3 1.53454401875263 1104 4 1.877143084998682 1104 5 1.5958576019958102 1104 6 1.52070693678764 1104 7 1.4293311256733503 1104 8 1.9411766259846779 1104 9 2.4556588283807166 1104 10-11 2.221638607740971 1104 12-13 1.619906793307031 1104 14-15 2.1887543670266325 1104 16-17 1.8541070571060878 1104 18-19 1.2116870957848036 1104 20-21 -3.472329298795806 1104 22-23 -0.171654832592381 1104 24-25 1.3714857386241377 1104 26-27 1.5763387105103845 1104 28-29 1.29056735745975 1104 30-31 2.119579416984802 1104 32-33 1.8905596034439185 1104 34-35 2.139434871104399 1104 36-37 2.2925545996397183 1104 38-39 2.2676643779608234 1104 40-41 2.3327340392664517 1104 42-43 2.3657330154261658 1104 44-45 2.514279156187321 1104 46-47 2.458546150324498 1104 48-49 2.404030946646465 1104 50-51 2.3625325199722873 1104 52-53 2.335229857046482 1104 54-55 2.3082079906538695 1104 56-57 2.587455640578032 1104 58-59 2.5143172891232766 1104 60-61 2.723060551925837 1104 62-63 2.4670542807453906 1104 64-65 2.484932137956136 1104 66-67 2.3485173567977142 1104 68-69 2.321104843663452 1104 70-71 2.526601541454731 1104 72-73 2.4486458471717967 1104 74-75 2.535208498995299 1104 76-77 2.3749972856498225 1104 78-79 2.359364910789221 1104 80-81 2.2175858786757345 1104 82-83 2.265218717200156 1104 84-85 2.211677240896133 1104 86-87 2.1715232999586433 1104 88-89 2.205960706422566 1104 90-91 2.2312089017166628 1104 92-93 1.9211589567177327 1104 94-95 2.0052762229207133 1104 96-97 2.0733321870670274 1104 98-99 1.9752940159745265 1104 100-101 1.4766391328025392 1104 102-103 1.9616729393511108 1104 104-105 2.0795290693736526 1104 106-107 2.2597218688598417 1104 108-109 2.323823707681374 1104 110-111 2.42130513289899 1104 112-113 2.36261497984626 1104 114-115 2.3179865594811844 1104 116-117 2.42261040555956 1104 118-119 2.424556079203956 1104 120-121 2.427715354754305 1104 122-123 2.1543447816230916 1104 124-125 2.26225554532056 1105 1 1.6630211830659363 1105 2 1.351570703116444 1105 3 1.3844399951388944 1105 4 2.1193187817777925 1105 5 1.6693806602265013 1105 6 1.620852818278717 1105 7 1.519633790769312 1105 8 1.7037893086037528 1105 9 2.1966994227221335 1105 10-11 2.025816884744792 1105 12-13 2.0988774130636045 1105 14-15 2.3221425960946647 1105 16-17 2.2595939344159994 1105 18-19 1.691668139857189 1105 20-21 1.9260934767425546 1105 22-23 1.8528345388577137 1105 24-25 1.9420724479825644 1105 26-27 1.9286097820084933 1105 28-29 1.5215960039626069 1105 30-31 1.8243086060512574 1105 32-33 1.8879138331694278 1105 34-35 1.7402795393814436 1105 36-37 2.0232903565508913 1105 38-39 1.8503182598239754 1105 40-41 1.8712137056975884 1105 42-43 2.0526379128267003 1105 44-45 2.176179186056917 1105 46-47 2.4304780314677714 1105 48-49 2.1054320733715493 1105 50-51 2.265552989023167 1105 52-53 2.267663298977272 1105 54-55 2.2658489165073625 1105 56-57 2.6136264912028935 1105 58-59 2.3069910628665724 1105 60-61 2.600735108518318 1105 62-63 2.4707554530548315 1105 64-65 2.1195639706263094 1105 66-67 2.259226300401952 1105 68-69 1.778492109047015 1105 70-71 2.073735446797574 1105 72-73 2.0069480061600906 1105 74-75 2.242717958182279 1105 76-77 2.0998709753884874 1105 78-79 1.8969133333336572 1105 80-81 1.9557789240861894 1105 82-83 1.99430537773884 1105 84-85 1.9211133468251624 1105 86-87 2.027360697271078 1105 88-89 2.1683084047015093 1105 90-91 2.08395904225398 1105 92-93 2.115491013034344 1105 94-95 1.976407604658199 1105 96-97 2.0889244341446336 1105 98-99 1.9372938230065344 1105 100-101 2.038707518579063 1105 102-103 1.923921351553858 1105 104-105 2.0570180820419424 1105 106-107 2.1704924004519945 1105 108-109 2.290042927408944 1105 110-111 2.1423066656657106 1105 112-113 2.1746733127992393 1105 114-115 2.1071671792550895 1105 116-117 2.192385753840785 1105 118-119 2.2460400608384887 1105 120-121 2.1887593058143864 1105 122-123 2.196114898969583 1105 124-125 1.8271533387257932 1106 1 -0.4415212689124317 1106 2 -0.3188261347267911 1106 3 -0.29851529555712375 1106 4 -0.5682748378319502 1106 5 -1.0760557402183117 1106 6 -0.5328053560913695 1106 7 -0.46642508503500935 1106 8 -0.0037008542088265983 1106 9 0.003915973908775072 1106 10-11 -2.361351763616156 1106 12-13 -0.6097775297666566 1106 14-15 -0.29831269460135346 1106 16-17 -0.4696924299116034 1106 18-19 -3.203263154279469 1106 20-21 -5.830829146887606 1106 22-23 -6.713712370744712 1106 24-25 -6.632051531468644 1106 26-27 -5.4872354557472995 1106 28-29 -2.003213608442703 1106 30-31 -4.0534137880445265 1106 32-33 -4.3707216253765715 1106 34-35 -3.7175156594057768 1106 36-37 -6.2407723068082 1106 38-39 -6.703075035206108 1106 40-41 -2.9557597909235476 1106 42-43 -1.3823186158342047 1106 44-45 -0.548874633338027 1106 46-47 -0.2396601643825491 1106 48-49 -0.011050324240912346 1106 50-51 -0.22620830710943807 1106 52-53 -0.4215020132940239 1106 54-55 -0.29803120049338716 1106 56-57 -0.20303417572989702 1106 58-59 0.019902252548455124 1106 60-61 0.24556268448326435 1106 62-63 -0.011143825076196379 1106 64-65 -0.21498986302542278 1106 66-67 -0.5260802325359926 1106 68-69 -0.42134619515493554 1106 70-71 -0.11572913932771556 1106 72-73 0.020236822656151787 1106 74-75 -0.1422081559861219 1106 76-77 -0.17454158532169117 1106 78-79 -0.3076184260496895 1106 80-81 -0.6202063985450472 1106 82-83 -0.6506670828886527 1106 84-85 -0.7975820021336162 1106 86-87 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-7.199419930626387 2216 102-103 -7.9925839571263335 2216 104-105 -10.48305296316652 2216 106-107 -7.5161061751397575 2216 108-109 -9.39420083386981 2216 110-111 -7.266876796247871 2216 112-113 -6.642550916157301 2216 114-115 -7.079274953794801 2216 116-117 -6.639544950070352 2216 118-119 -6.922104280949409 2216 120-121 -7.617769085988403 2216 122-123 -10.117760744174978 2216 124-125 -8.84463393153801 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 283686.0 3 41156.0 4 23669.0 5 19269.0 6 20368.0 7 22574.0 8 24711.0 9 25999.0 10 27226.0 11 27784.0 12 28197.0 13 29023.0 14 30003.0 15 25584.0 16 21322.0 17 19197.0 18 17052.0 19 16504.0 20 16138.0 21 17439.0 22 21528.0 23 28866.0 24 38759.0 25 48511.0 26 57602.0 27 64247.0 28 72344.0 29 81066.0 30 89449.0 31 100176.0 32 116079.0 33 138177.0 34 166419.0 35 202244.0 36 249092.0 37 247781.0 38 64735.0 39 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18271969617049 20.63354133831255 14.334774059317509 22.84896490619946 2 14.489488239812237 21.81902635331531 40.602154270771656 23.0893311361008 3 17.87782770276698 28.174328232317148 29.556668209866753 24.39117585504912 4 10.718252543102526 17.774841777044173 39.50289887129138 32.00400680856192 5 12.371907241219922 40.09068471798385 34.29690801423618 13.240500026560046 6 28.902986603253368 39.107303530825035 18.635435985595713 13.354273880325884 7 26.885549479219286 33.946328821841846 22.214430354370187 16.95369134456868 8 25.35536761798506 35.993456433938746 20.798524250734747 17.85265169734145 9 26.2820337570096 16.97395379539523 21.45811516561332 35.28589728198185 10-11 23.18858675628179 28.53334712759314 28.28883594482851 19.989230171296562 12-13 24.26121217308064 26.33788904631945 28.415497031535775 20.985401749064135 14-15 22.118693597757282 27.41657668723811 26.139694301082727 24.32503541392188 16-17 21.06458566973255 30.79806774005635 26.706717337634288 21.430629252576807 18-19 21.02649098474341 29.34672082425203 28.939449177729344 20.687339013275214 20-21 21.90036428961937 28.50492874095128 28.961897575184825 20.632809394244525 22-23 21.844409066908124 28.534131672460372 28.43881045620841 21.182648804423096 24-25 21.411867825662444 28.868136525227733 28.687186251979547 21.032809397130276 26-27 21.202776964971356 28.950372934516906 28.59335665631007 21.25349344420167 28-29 21.19389312856119 28.466534675747283 29.009382660482085 21.330189535209442 30-31 21.406767751545225 28.381137047394706 28.924583332425378 21.28751186863469 32-33 21.185658651044054 28.69410899086044 28.67532825672392 21.444904101371588 34-35 21.500874861776595 28.480213224153438 28.829088009846483 21.18982390422349 36-37 21.73191353051379 28.126771146436035 28.528044460195673 21.613270862854502 38-39 21.263425287921063 28.698001649904786 28.60881011117442 21.429762950999727 40-41 21.36362807686149 28.445561725880115 28.987336655057018 21.203473542201372 42-43 21.343756129551224 28.375498041515513 28.559617377314865 21.721128451618398 44-45 21.205109180257054 28.537125174757634 28.5823851448924 21.67538050009292 46-47 21.498162198056292 28.60364295650568 28.208110417882526 21.6900844275555 48-49 21.353714714550513 28.6966067484648 28.034364408223556 21.915314128761132 50-51 21.50667754743645 28.69698026939217 27.87553366193575 21.920808521235628 52-53 21.63059978420496 28.892760643042276 27.809882430139034 21.666757142613736 54-55 21.647049313846672 28.72643356981405 27.72304316979915 21.90347394654013 56-57 21.435592938958596 28.972449925253883 27.6864522320224 21.90550490376512 58-59 21.351051860035184 28.401062984801566 28.05452106345238 22.193364091710873 60-61 21.5765950894837 28.696768425136842 28.02107802125637 21.705558464123094 62-63 21.460460352381453 28.70065406609604 28.135282621336046 21.703602960186462 64-65 21.408710760321853 28.499553754062525 27.9333315503307 22.15840393528493 66-67 21.303947030321737 28.879993073890848 27.56040682570434 22.255653070083074 68-69 21.314723474125667 28.631360315014103 27.901713412209688 22.15220279865054 70-71 21.25573977102922 28.402860361739044 28.36642932011435 21.97497054711738 72-73 21.487233145644517 28.522057530082773 27.80225797096396 22.18845135330875 74-75 21.245595831109707 28.843468121800502 27.765073222217335 22.145862824872456 76-77 21.214790912316523 28.586176544530375 28.195936855581007 22.0030956875721 78-79 21.20378017058955 28.357362281670895 28.127184479869893 22.31167306786966 80-81 21.245449836020757 28.904563189210553 27.665888454494397 22.184098520274294 82-83 21.452461529409643 28.774081944729772 27.69539783236991 22.07805869349067 84-85 21.267300167514275 28.49874245432978 27.757468014599258 22.47648936355669 86-87 21.2042409115753 28.561413045523892 28.057537852051468 22.176808190849343 88-89 21.491335292502473 28.32590885623959 27.970996002550063 22.21175984870787 90-91 21.326525619165405 28.558840350940795 28.016564830283592 22.098069199610208 92-93 21.478068006770247 28.460778673715843 27.829316667617544 22.231836651896366 94-95 21.218703061773926 28.00391770132995 28.11650010757038 22.660879129325746 96-97 21.537688042033224 28.078764550049158 27.831676937056542 22.551870470861076 98-99 21.138393860915755 28.10445254767361 27.94103867356033 22.816114917850307 100-101 21.0850606554477 28.175736671936804 28.059095008873918 22.680107663741577 102-103 21.27915960127415 27.883274433051774 28.16011235955056 22.677453606123514 104-105 21.031971803196072 28.06249192741288 28.17794478073071 22.72759148866034 106-107 20.917139865909547 28.142949256995475 28.177536630227106 22.76237424686787 108-109 21.032445578523046 28.206062812731957 27.728591597592178 23.032900011152815 110-111 20.793002227017958 28.209990241691475 28.00890094029207 22.988106590998505 112-113 20.75691890032948 28.18834797437039 28.341540026846744 22.71319309845338 114-115 20.761603690645376 28.51617054337891 27.867954198152695 22.854271567823016 116-117 20.50122130720482 28.49901047372456 28.0655603254442 22.93420789362642 118-119 20.704432771494478 28.09537657677932 28.01484880589103 23.18534184583518 120-121 20.52875188152243 28.350853267202535 28.058090556352166 23.06230429492287 122-123 20.61939567597379 28.350807307927596 28.11132101187665 22.91847600422196 124-125 20.589679284539063 28.361165777480647 27.846880165129544 23.202274772850746 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1189.0 1 1239.0 2 1477.5 3 1526.0 4 1231.0 5 929.5 6 819.5 7 934.5 8 1129.5 9 1251.0 10 1234.0 11 1229.5 12 1272.0 13 1412.5 14 1591.0 15 1734.0 16 1950.0 17 2176.0 18 2493.0 19 2921.0 20 3417.5 21 4108.0 22 5117.5 23 6467.0 24 8046.5 25 10622.5 26 15104.0 27 20202.5 28 24072.5 29 28934.0 30 36109.5 31 43453.0 32 52184.5 33 62088.0 34 72026.5 35 81242.5 36 91993.5 37 100384.0 38 101241.0 39 100320.0 40 96313.5 41 91453.5 42 88283.0 43 87383.5 44 89052.5 45 89356.0 46 86860.0 47 83126.0 48 80820.0 49 78403.0 50 75039.0 51 71241.5 52 67924.5 53 65721.0 54 62879.0 55 58228.5 56 53122.0 57 48408.5 58 44589.5 59 40714.0 60 35389.0 61 29524.0 62 23993.0 63 18591.0 64 14132.5 65 10928.5 66 8330.0 67 6325.5 68 4789.5 69 3563.5 70 2761.0 71 2204.5 72 1790.0 73 1581.0 74 1287.5 75 960.5 76 732.5 77 534.0 78 387.5 79 276.5 80 201.0 81 142.5 82 101.0 83 73.5 84 63.0 85 50.0 86 34.5 87 25.0 88 22.5 89 20.5 90 11.0 91 5.5 92 6.5 93 5.5 94 3.5 95 4.0 96 3.0 97 2.0 98 1.5 99 1.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10494983683053868 2 0.035220990918214005 3 0.046234979079365295 4 0.053762524944900345 5 0.05851676443892249 6 0.003644916945416973 7 0.002535594396811807 8 0.002258263759660516 9 0.008002969814937266 10-11 0.05049398529276012 12-13 0.007884113827586713 14-15 0.013391107908162355 16-17 0.023493866832959398 18-19 0.022305306959453864 20-21 0.14028968373610326 22-23 0.01943295393181549 24-25 0.01749163947175645 26-27 0.009052864369867155 28-29 0.006022036692428042 30-31 0.0031100650023394823 32-33 0.007923732490036896 34-35 0.02066113246777121 36-37 0.003862819588892987 38-39 0.01113284414850184 40-41 0.03466632964391142 42-43 0.01695678752867896 44-45 0.12168872171574165 46-47 0.004912714143822877 48-49 0.011449793448103316 50-51 0.013648629214088555 52-53 0.014738142431468627 54-55 0.012658162652833942 56-57 0.007369071215734314 58-59 0.01642193558560147 60-61 0.03430976168185976 62-63 0.009330195007018446 64-65 0.009132101694767523 66-67 0.011073416154826564 68-69 0.009488669656819184 70-71 0.004615574175446492 72-73 0.0010697038861549812 74-75 0.0013470345233062724 76-77 0.009092483032317338 78-79 0.022939205558656816 80-81 0.004575955512996308 82-83 0.006121083348553503 84-85 0.003922247582568264 86-87 0.004932523475047968 88-89 0.008082207139837636 90-91 0.006398413985704794 92-93 0.003129874333564574 94-95 0.005308900768324721 96-97 0.002357310415785977 98-99 0.004100531563594094 100-101 0.0037043449390922497 102-103 9.112292363542432E-4 104-105 0.003526060958066419 106-107 8.12182580228782E-4 108-109 0.0014460811794317337 110-111 0.002377119747011069 112-113 0.00423919688216974 114-115 0.002377119747011069 116-117 0.005071188793623614 118-119 0.003526060958066419 120-121 0.00697288459123247 122-123 0.004714620831571954 124-125 0.056951827272140196 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2524063.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.09286242748039 #Duplication Level Percentage of deduplicated Percentage of total 1 80.73080254814262 44.47690998444905 2 9.299380493250109 10.24658980350846 3 3.6750445918695402 6.074061783441733 4 1.9192014252232659 4.229372003617987 5 1.1151673887068645 3.0718881764819916 6 0.6793365102352025 2.2455955740211584 7 0.4937495278296909 1.904145236724819 8 0.3419420775372165 1.5070854268739764 9 0.25347581842735556 1.2568237553980168 >10 1.3216672663003286 13.021284805245134 >50 0.10034291470807429 3.828446318389031 >100 0.06625144038382087 6.585804508132738 >500 0.003096167988025775 1.0870003840886981 >1k 5.41829397904375E-4 0.4649922396273336 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2776 0.10998140696171213 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.961866245018448E-5 0.0 2 3.961866245018448E-5 0.0 0.0 3.961866245018448E-5 0.0 3 3.961866245018448E-5 0.0 0.0 3.961866245018448E-5 0.0 4 3.961866245018448E-5 0.0 0.0 7.923732490036897E-5 0.0 5 1.1885598735055345E-4 0.0 0.0 1.1885598735055345E-4 0.0 6 1.5847464980073793E-4 0.0 0.0 1.1885598735055345E-4 0.0 7 2.7733063715129137E-4 0.0 0.0 1.1885598735055345E-4 0.0 8 3.1694929960147586E-4 0.0 0.0 1.1885598735055345E-4 0.0 9 3.1694929960147586E-4 0.0 0.0 1.1885598735055345E-4 0.0 10-11 3.1694929960147586E-4 0.0 0.0 1.1885598735055345E-4 0.0 12-13 3.763772932767526E-4 0.0 0.0 1.1885598735055345E-4 0.0 14-15 5.546612743025827E-4 0.0 0.0 1.1885598735055345E-4 0.0 16-17 5.546612743025827E-4 0.0 0.0 1.1885598735055345E-4 0.0 18-19 6.338985992029517E-4 0.0 0.0 1.9809331225092243E-4 0.0 20-21 7.131359241033207E-4 0.0 0.0 2.575213059261991E-4 0.0 22-23 7.527545865535052E-4 0.0 0.0 4.159959557269371E-4 0.0 24-25 0.0011093225486051655 0.0 0.0 8.319919114538742E-4 0.0 26-27 0.0011291318798302577 0.0 0.0 0.001723411816583025 0.0 28-29 0.0013668438545313647 0.0 0.0 0.0031496836647896664 0.0 30-31 0.0015055091731070104 0.0 0.0 0.008458584433114388 0.0 32-33 0.001545127835557195 0.0 0.0 0.01917543262588929 0.0 34-35 0.001723411816583025 0.0 0.0 0.033537197764081164 0.0 36-37 0.0018620771351586707 0.0 0.0 0.057070683259490745 0.0 38-39 0.002099789109859778 0.0 0.0 0.09732324430887818 0.0 40-41 0.002099789109859778 0.0 0.0 0.14545991918585233 0.0 42-43 0.0023176917533357924 0.0 0.0 0.19032805441068626 0.0 44-45 0.0025752130592619914 0.0 0.0 0.24405096069313642 0.0 46-47 0.0029119716900885594 0.0 0.0 0.30837185918101095 0.0 48-49 0.00309025567111439 0.0 0.0 0.3823002833130552 0.0 50-51 0.00309025567111439 0.0 0.0 0.4631817827051068 0.0 52-53 0.0031100650023394823 0.0 0.0 0.5418050183374978 0.0 54-55 0.0031298743335645743 0.0 0.0 0.6275199945484721 0.0 56-57 0.003407204970715866 0.0 0.0 0.7266656973300587 0.0 58-59 0.003407204970715866 0.0 0.0 0.8421937170347966 0.0 60-61 0.003407204970715866 0.0 0.0 0.9711920819725973 0.0 62-63 0.0035260609580664193 3.961866245018448E-5 0.0 1.1027062319759846 0.0 64-65 0.003565679620516604 3.961866245018448E-5 0.0 1.2374691123002872 0.0 66-67 0.003763772932767526 3.961866245018448E-5 0.0 1.3974492712741322 0.0 68-69 0.0038033915952177103 3.961866245018448E-5 0.0 1.5849842099820806 0.0 70-71 0.003902438251343172 3.961866245018448E-5 0.0 1.7946461716684567 0.0 72-73 0.003981675576243541 3.961866245018448E-5 0.0 2.0157381174717113 0.0 74-75 0.004001484907468633 3.961866245018448E-5 0.0 2.232056014449719 0.0 76-77 0.004041103569918818 3.961866245018448E-5 0.0 2.4678068653595413 0.0 78-79 0.004041103569918818 3.961866245018448E-5 0.0 2.7485447074815488 0.0 80-81 0.004714620831571954 3.961866245018448E-5 0.0 3.061987755456183 0.0 82-83 0.004793858156472323 3.961866245018448E-5 0.0 3.3878116354465004 0.0 84-85 0.004952332806273061 3.961866245018448E-5 0.0 3.714328842029696 0.0 86-87 0.005170235449749075 3.961866245018448E-5 0.0 4.051760990117917 0.0 88-89 0.005249472774649444 3.961866245018448E-5 0.0 4.425642307660308 0.0 90-91 0.00556642207425092 3.961866245018448E-5 0.0 4.8519985436179685 0.0 92-93 0.005823943380177119 3.961866245018448E-5 0.0 5.309851616223526 0.0 94-95 0.005843752711402211 3.961866245018448E-5 0.0 5.771151512462248 0.0 96-97 0.005863562042627304 3.961866245018448E-5 0.0 6.233580540580801 0.0 98-99 0.006101274017328411 3.961866245018448E-5 0.0 6.724851954963089 3.961866245018448E-5 100-101 0.006319176660804425 3.961866245018448E-5 0.0 7.280186746527326 3.961866245018448E-5 102-103 0.006517269973055347 3.961866245018448E-5 0.0 7.883222407681583 3.961866245018448E-5 104-105 0.00750773653430996 3.961866245018448E-5 0.0 8.483207431827177 3.961866245018448E-5 106-107 0.007923732490036896 3.961866245018448E-5 0.0 9.088897543365597 3.961866245018448E-5 108-109 0.008082207139837636 3.961866245018448E-5 0.0 9.704036705898387 3.961866245018448E-5 110-111 0.008319919114538742 3.961866245018448E-5 0.0 10.393322195206697 3.961866245018448E-5 112-113 0.008418965770664203 3.961866245018448E-5 0.0 11.128426667638646 3.961866245018448E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4125 0.0 52.816822 1 GTATCAA 7845 0.0 49.700813 1 TCAACGC 9435 0.0 40.389996 4 ATCAACG 9510 0.0 40.16561 3 CAACGCA 9725 0.0 39.42891 5 GTACATG 11980 0.0 39.30382 1 AACGCAG 9570 0.0 38.99282 6 TATCAAC 10470 0.0 37.27838 2 TACATGG 12710 0.0 35.576885 2 ACGCAGA 11340 0.0 32.697357 7 ACATGGG 12820 0.0 32.16215 3 CGCAGAG 11200 0.0 32.047726 8 CATGGGA 8285 0.0 28.172754 4 GCAGAGT 11705 0.0 27.308687 9 GAGTACT 4460 0.0 25.275078 12-13 ACGCATA 400 0.0 23.80992 8 AGAGTAC 10710 0.0 23.669022 10-11 ATGGGAT 3805 0.0 23.628754 5 CAGAGTA 11310 0.0 23.491947 10-11 CATGGGG 4945 0.0 23.11909 4 >>END_MODULE