FastQCFastQC Report
Thu 26 May 2016
SRR1364483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524063
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85160.337392529425771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78600.3114026868584501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47230.18711894275222132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70200.067.451581
GTATCAA126550.048.302591
TCAACGC158350.036.054074
ATCAACG161150.035.9453543
TATCAAC168100.035.5582282
CAACGCA161600.035.1965265
AACGCAG164900.034.0231326
GTACATG120700.032.7084121
TACATGG130550.029.6261122
ACGCAGA191800.028.9411837
CGCAGAG194500.028.8950448
ACATGGG127050.027.9093113
GAGTACT126150.026.87245412-13
GCAGAGT202950.026.6956489
GTACTTT135200.025.33760514-15
TATAACG1951.4458274E-624.4490622
CAGAGTA197200.024.36824210-11
AGAGTAC191150.024.2994510-11
AGTACTT138550.024.05962612-13
CATGGGA83900.023.7241884
Produced by FastQC (version 0.11.5)