##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364483_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2524063 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.94905436195531 31.0 28.0 31.0 25.0 34.0 2 29.09526862047421 31.0 28.0 33.0 25.0 34.0 3 29.177707133300554 31.0 28.0 34.0 25.0 34.0 4 32.63426229852424 35.0 32.0 37.0 26.0 37.0 5 31.817593300959604 35.0 32.0 37.0 22.0 37.0 6 31.864535473163706 35.0 32.0 37.0 22.0 37.0 7 31.330799191620812 35.0 31.0 37.0 17.0 37.0 8 31.417713424744154 35.0 32.0 37.0 17.0 37.0 9 31.928331424374115 37.0 30.0 39.0 11.0 39.0 10-11 32.12971110467528 37.0 30.5 39.0 16.5 39.0 12-13 31.918284527763376 37.0 30.5 39.0 13.5 39.0 14-15 32.83162642929277 37.0 31.0 40.0 14.5 41.0 16-17 32.89884662149875 37.0 31.0 40.0 16.0 41.0 18-19 32.79012389944308 37.5 31.0 40.0 12.0 41.0 20-21 32.6966264312737 37.5 31.0 40.0 11.5 41.0 22-23 32.68623683323277 37.0 31.0 40.0 11.5 41.0 24-25 32.484888451674934 37.0 30.5 40.0 10.0 41.0 26-27 32.5479722574278 37.0 31.0 40.0 10.0 41.0 28-29 32.40950404169785 37.0 30.5 40.0 10.0 41.0 30-31 31.869234444623608 37.0 30.0 40.0 8.5 41.0 32-33 31.746339730822882 36.5 30.0 40.0 8.0 41.0 34-35 31.51678345588046 36.0 30.0 40.0 7.0 41.0 36-37 31.464315668824433 36.0 30.0 40.0 2.0 41.0 38-39 31.21130593808475 36.0 30.0 40.0 2.0 41.0 40-41 30.907654642534673 36.0 29.0 40.0 2.0 41.0 42-43 30.76136193906412 36.0 28.5 40.0 2.0 41.0 44-45 30.640723904276555 36.0 28.5 40.0 2.0 41.0 46-47 30.395960798125877 36.0 28.0 40.0 2.0 41.0 48-49 30.054021036717387 36.0 27.0 40.0 2.0 41.0 50-51 29.695595157490125 35.0 26.5 40.0 2.0 41.0 52-53 29.609146443650573 35.0 26.5 40.0 2.0 41.0 54-55 29.14495359267974 35.0 25.0 39.5 2.0 41.0 56-57 29.178181170596773 35.0 26.0 39.0 2.0 41.0 58-59 28.948767126652545 35.0 25.5 39.0 2.0 41.0 60-61 28.6804146726924 34.5 25.0 39.0 2.0 41.0 62-63 28.422744004408763 34.0 24.5 39.0 2.0 40.5 64-65 27.960354198766037 34.0 23.0 38.0 2.0 40.0 66-67 27.571267040481953 34.0 22.0 38.0 2.0 40.0 68-69 27.388854200548877 34.0 22.0 37.5 2.0 40.0 70-71 27.463785769214162 34.0 22.5 37.0 2.0 40.0 72-73 27.221986139014753 34.0 22.0 37.0 2.0 39.0 74-75 26.862212432890935 33.0 21.5 36.0 2.0 39.0 76-77 26.774512957877832 33.0 22.0 36.0 2.0 39.0 78-79 26.365614289342222 33.0 21.0 35.5 2.0 38.0 80-81 26.1283127639841 33.0 20.0 35.0 2.0 37.0 82-83 25.965244132178952 33.0 20.0 35.0 2.0 37.0 84-85 25.518985064952815 32.5 18.0 35.0 2.0 36.0 86-87 25.385585660896737 32.0 18.0 35.0 2.0 36.0 88-89 25.32687476501181 32.0 19.0 35.0 2.0 36.0 90-91 25.154335291947945 32.0 18.0 35.0 2.0 35.0 92-93 25.157603237320146 32.0 18.5 35.0 2.0 35.0 94-95 25.15223371999827 32.0 18.5 35.0 2.0 35.0 96-97 25.02038281136406 32.0 18.0 35.0 2.0 35.0 98-99 24.8825629550451 32.0 16.0 35.0 2.0 35.0 100-101 24.787278289012598 32.0 13.5 35.0 2.0 35.0 102-103 23.64950359796883 30.0 10.5 34.0 2.0 35.0 104-105 24.58917229878969 32.0 15.0 34.5 2.0 35.0 106-107 24.69513142104615 32.0 10.0 35.0 2.0 35.0 108-109 24.76146118381356 32.5 10.0 35.0 2.0 35.0 110-111 24.68084592183317 32.5 7.5 35.0 2.0 35.0 112-113 24.617879189227843 32.5 7.0 35.0 2.0 35.0 114-115 24.55725174054689 32.5 3.5 35.0 2.0 35.0 116-117 24.468695908144923 32.5 2.0 35.0 2.0 35.0 118-119 24.340347487364618 32.5 2.0 35.0 2.0 35.0 120-121 24.16198268426739 32.0 2.0 35.0 2.0 35.0 122-123 23.863106229915815 32.0 2.0 35.0 2.0 35.0 124-125 23.07900852712472 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.991884525985828 1101 2 0.9928335874940153 1101 3 0.9812779924727337 1101 4 1.0026785649828511 1101 5 1.1859880602167436 1101 6 1.2788486203570208 1101 7 1.6054830996071416 1101 8 1.448355006771294 1101 9 2.052572785907639 1101 10-11 1.8471742371689501 1101 12-13 2.018396664410865 1101 14-15 1.929885847166588 1101 16-17 1.9507619850337505 1101 18-19 1.9783426661249308 1101 20-21 2.18867248393655 1101 22-23 2.2143723478405732 1101 24-25 2.204849394852893 1101 26-27 2.0070612692326506 1101 28-29 2.032936187681514 1101 30-31 2.426372057338245 1101 32-33 2.310426814686142 1101 34-35 2.321733897290322 1101 36-37 2.185732641310505 1101 38-39 2.342701057607268 1101 40-41 2.516758441359695 1101 42-43 2.4349618586679114 1101 44-45 2.2008806880920915 1101 46-47 2.1004848795400832 1101 48-49 2.1456023764106966 1101 50-51 2.4194338221607836 1101 52-53 2.1881995689277787 1101 54-55 2.4728594361796716 1101 56-57 2.3081106546311325 1101 58-59 2.5437046991876677 1101 60-61 2.624316000805834 1101 62-63 2.5303352250051105 1101 64-65 2.705293947687249 1101 66-67 2.8848066561941152 1101 68-69 2.8199830134172004 1101 70-71 2.4837413900349574 1101 72-73 2.6521216793112394 1101 74-75 2.765004882898314 1101 76-77 2.512199803839483 1101 78-79 2.5772258288748766 1101 80-81 2.6257639627698666 1101 82-83 2.4960471813716865 1101 84-85 2.772548616860483 1101 86-87 2.5938688651595925 1101 88-89 2.449786186937068 1101 90-91 2.441873522760119 1101 92-93 2.299144557823933 1101 94-95 2.256362871321034 1101 96-97 2.166394448253129 1101 98-99 2.2269537414744462 1101 100-101 2.356538588095418 1101 102-103 2.1496715668144084 1101 104-105 2.1456610285926736 1101 106-107 2.2654292552164 1101 108-109 2.2319662772768787 1101 110-111 2.250874476144862 1101 112-113 2.0969737797253316 1101 114-115 2.0866875464488324 1101 116-117 2.1572318755400737 1101 118-119 2.0673890586054284 1101 120-121 2.0692826442233496 1101 122-123 2.0412285498424687 1101 124-125 1.848851227747975 1102 1 0.5553603171658708 1102 2 -1.5137793129908914 1102 3 0.19507011036181865 1102 4 0.55761317790747 1102 5 1.028992274868699 1102 6 0.5729260652332009 1102 7 1.4873395438279644 1102 8 1.1705281569779658 1102 9 1.9140680994993637 1102 10-11 1.6429385094906834 1102 12-13 1.685629752667353 1102 14-15 1.7814579926345147 1102 16-17 1.7204732347040306 1102 18-19 1.720761316791041 1102 20-21 1.9150738009136958 1102 22-23 1.8750823367848994 1102 24-25 2.0528061272502 1102 26-27 1.7605166595072532 1102 28-29 1.8100392406381687 1102 30-31 2.10996364369797 1102 32-33 1.7200888975216806 1102 34-35 1.9310537874773743 1102 36-37 1.370387033939334 1102 38-39 1.369376931587876 1102 40-41 1.5513994361584302 1102 42-43 1.354429878613928 1102 44-45 1.320374309222636 1102 46-47 1.0485172456436729 1102 48-49 1.16982024729354 1102 50-51 1.3906614586825796 1102 52-53 1.0811702089623196 1102 54-55 1.371572887379429 1102 56-57 0.9920721898057359 1102 58-59 0.9350414319741027 1102 60-61 1.0581805310111676 1102 62-63 1.372477662410276 1102 64-65 1.4693680110890313 1102 66-67 1.6048086929598284 1102 68-69 1.4929609138934872 1102 70-71 1.0214337775799969 1102 72-73 1.2558861801841879 1102 74-75 1.411605282025583 1102 76-77 1.3597905587456367 1102 78-79 1.181508171889373 1102 80-81 1.2387032681367032 1102 82-83 0.8887195103455525 1102 84-85 0.9434362157156571 1102 86-87 1.0433180086959126 1102 88-89 1.2367262087430184 1102 90-91 0.9763964245527958 1102 92-93 0.9692239769097775 1102 94-95 0.9294061908895763 1102 96-97 1.0191633167201815 1102 98-99 0.7666478301006023 1102 100-101 0.6654053348535243 1102 102-103 0.5596885135917731 1102 104-105 0.8410565281039233 1102 106-107 1.0980945441723762 1102 108-109 0.9516867502695376 1102 110-111 0.8320372193899779 1102 112-113 0.8371602693928679 1102 114-115 1.109108501098408 1102 116-117 0.875196099885752 1102 118-119 0.4477893200108838 1102 120-121 0.6417648742429094 1102 122-123 1.0494072601486266 1102 124-125 1.219032965372655 1103 1 0.7749724849209194 1103 2 0.6987716461819815 1103 3 0.5633304529609902 1103 4 0.7105071200862056 1103 5 0.9695144407710217 1103 6 0.9089831262203383 1103 7 1.2380304405986848 1103 8 1.0512253795877058 1103 9 1.8373529076684783 1103 10-11 1.5521786817424683 1103 12-13 1.5379652384098534 1103 14-15 1.3055272749654918 1103 16-17 1.2314423281456115 1103 18-19 1.449436522233185 1103 20-21 1.6036665674607065 1103 22-23 1.5740892095838674 1103 24-25 1.8219681787116784 1103 26-27 1.3651730343216997 1103 28-29 1.4677739813046031 1103 30-31 2.1015645607600995 1103 32-33 1.898362131693606 1103 34-35 1.9465576291143876 1103 36-37 1.9174057516319714 1103 38-39 1.9745494491020317 1103 40-41 2.2240548897771895 1103 42-43 2.142225475032781 1103 44-45 2.0040509872492436 1103 46-47 1.8988998961628454 1103 48-49 1.9808727133742714 1103 50-51 2.1673101627713436 1103 52-53 1.9906788787572154 1103 54-55 2.2353232093919466 1103 56-57 2.1556703263036106 1103 58-59 2.2093924983245508 1103 60-61 2.1904999954775946 1103 62-63 2.2708886182910533 1103 64-65 2.32267276373544 1103 66-67 2.6057097744051916 1103 68-69 2.631522161966025 1103 70-71 2.3174894102709303 1103 72-73 2.431374790352333 1103 74-75 2.4726007269786052 1103 76-77 2.312080482946289 1103 78-79 2.3578933424102253 1103 80-81 2.3467489861637247 1103 82-83 2.2493987187077664 1103 84-85 2.3667761996685464 1103 86-87 2.2626208045396083 1103 88-89 2.164827180058257 1103 90-91 2.2124752421449756 1103 92-93 2.0390975560872135 1103 94-95 1.9996097175632812 1103 96-97 1.945330552121849 1103 98-99 2.011640673856803 1103 100-101 2.1281724760688405 1103 102-103 1.9175213711954378 1103 104-105 1.937101420893132 1103 106-107 1.905570918233927 1103 108-109 1.9647458802563023 1103 110-111 2.0036242188681186 1103 112-113 1.8348376272441804 1103 114-115 1.740630684716038 1103 116-117 1.7913901739696705 1103 118-119 1.883636399311218 1103 120-121 1.810639956268453 1103 122-123 1.9405101926954025 1103 124-125 1.7767263518814183 1104 1 0.8592665984895653 1104 2 0.7181778735534508 1104 3 0.7355585200627033 1104 4 0.7133989113725079 1104 5 1.028442678788707 1104 6 1.1770754640004846 1104 7 1.5199076221795735 1104 8 1.336242340286816 1104 9 1.8754134587020452 1104 10-11 1.4689067268359821 1104 12-13 1.6231681612140036 1104 14-15 1.6626520200371644 1104 16-17 1.5654273631444084 1104 18-19 1.5053714723206042 1104 20-21 1.8915298933687907 1104 22-23 1.8983526612695343 1104 24-25 2.1082161889833273 1104 26-27 2.0708749995533466 1104 28-29 1.951043864851016 1104 30-31 2.3483066421950447 1104 32-33 2.27119540993451 1104 34-35 2.2271036453854904 1104 36-37 2.140799088239202 1104 38-39 2.338403180009916 1104 40-41 2.5323981555697976 1104 42-43 2.4925790878443763 1104 44-45 2.300280852981956 1104 46-47 2.388844306036674 1104 48-49 2.1396071641488312 1104 50-51 2.414504980228422 1104 52-53 2.3259599810199703 1104 54-55 2.6299330413902595 1104 56-57 2.5860978003634223 1104 58-59 2.6122919996733778 1104 60-61 2.677666193642885 1104 62-63 2.588160711863594 1104 64-65 2.703793611197895 1104 66-67 2.8142041919459295 1104 68-69 2.8882532623156507 1104 70-71 2.6848266509949035 1104 72-73 2.8353729180729665 1104 74-75 2.7692895428136914 1104 76-77 2.736676359156057 1104 78-79 2.87049808634265 1104 80-81 2.654625304102538 1104 82-83 2.6545444086821206 1104 84-85 2.9458235293833255 1104 86-87 2.8371227359390403 1104 88-89 2.7435424880134 1104 90-91 2.7224315508317147 1104 92-93 2.519348087089 1104 94-95 2.428187323226382 1104 96-97 2.459684530873723 1104 98-99 2.5021412151434745 1104 100-101 2.5336086167567657 1104 102-103 2.4894389932050203 1104 104-105 2.3212571273319256 1104 106-107 2.410124544451822 1104 108-109 2.367409073942568 1104 110-111 2.4414562586083086 1104 112-113 2.436205933190969 1104 114-115 2.399800754023172 1104 116-117 2.3618682582607917 1104 118-119 2.209219393675106 1104 120-121 2.332025627117254 1104 122-123 2.507172256339665 1104 124-125 2.302751811773703 1105 1 0.5890528257746617 1105 2 0.643335411120102 1105 3 0.4623272248141639 1105 4 0.7295364085776512 1105 5 0.8904265399626823 1105 6 0.9880455210300347 1105 7 1.2651797041597703 1105 8 1.1930838355692117 1105 9 2.0055252472553455 1105 10-11 1.586974626148283 1105 12-13 1.6808152400888048 1105 14-15 1.652942581302483 1105 16-17 1.4471772419616542 1105 18-19 1.5909127099849485 1105 20-21 1.9099117989797136 1105 22-23 1.8832713749472134 1105 24-25 2.0884896893360363 1105 26-27 1.857034514753039 1105 28-29 1.844198383104107 1105 30-31 2.176542134786292 1105 32-33 2.025398443460677 1105 34-35 2.04792867599172 1105 36-37 1.9627756582273683 1105 38-39 2.049970657854711 1105 40-41 2.219813493751982 1105 42-43 2.148969910425322 1105 44-45 2.1221452819902424 1105 46-47 1.9279003921258209 1105 48-49 1.9159716760640286 1105 50-51 2.116878573194221 1105 52-53 2.039797172653941 1105 54-55 2.264029070120536 1105 56-57 2.0507356936151453 1105 58-59 2.1590840512408995 1105 60-61 1.9417176144130615 1105 62-63 1.8690431013388675 1105 64-65 2.2716169206914003 1105 66-67 2.509314591014139 1105 68-69 2.4466076497664915 1105 70-71 2.3564186427076805 1105 72-73 2.53698030100524 1105 74-75 2.497620941652535 1105 76-77 2.2110441145512176 1105 78-79 2.3224711262876063 1105 80-81 2.3115351470300567 1105 82-83 2.22312786185012 1105 84-85 2.5317583004244923 1105 86-87 2.3659052985655293 1105 88-89 2.2881046037745527 1105 90-91 2.266441021284791 1105 92-93 2.1127577101832244 1105 94-95 1.9095359268371936 1105 96-97 2.038351572691731 1105 98-99 2.160699894456666 1105 100-101 2.1763138200173486 1105 102-103 1.8976343264994533 1105 104-105 1.578605279143428 1105 106-107 1.9124305986203645 1105 108-109 1.9608031267152697 1105 110-111 2.000611001027817 1105 112-113 2.0361940315722933 1105 114-115 1.783777737679241 1105 116-117 1.8573268952250217 1105 118-119 1.911834918816698 1105 120-121 1.840322181945293 1105 122-123 1.9400767077183758 1105 124-125 1.8176511362124081 1106 1 0.40244387535978987 1106 2 0.42798179512207923 1106 3 0.4051041392638979 1106 4 0.28634961131875514 1106 5 0.3660837781366695 1106 6 0.6019375780952281 1106 7 0.8100189825759401 1106 8 0.5894809062734012 1106 9 1.2777995859777498 1106 10-11 1.0028316257585956 1106 12-13 0.7698805171933429 1106 14-15 0.9807642977253579 1106 16-17 0.9418979513797012 1106 18-19 1.0013041302467016 1106 20-21 0.7980837097702036 1106 22-23 0.8094942513978367 1106 24-25 0.4412670314895806 1106 26-27 0.5878660778678721 1106 28-29 0.7605436778870711 1106 30-31 1.10051237848241 1106 32-33 0.9200686600688961 1106 34-35 0.9435758987683727 1106 36-37 1.0477743038319964 1106 38-39 1.1355549012466888 1106 40-41 1.2964813603654228 1106 42-43 1.3055564301519738 1106 44-45 1.300939608736936 1106 46-47 1.076362856365023 1106 48-49 1.138986239378447 1106 50-51 1.5648290997451184 1106 52-53 1.2006300188801688 1106 54-55 1.59789881530412 1106 56-57 1.3016190207705343 1106 58-59 1.3311906944313812 1106 60-61 1.5703443644344475 1106 62-63 1.7153750660200373 1106 64-65 1.7855497284242858 1106 66-67 1.829083892336218 1106 68-69 1.7212884758051032 1106 70-71 1.5590866827787764 1106 72-73 1.4279922244367533 1106 74-75 1.794165094941576 1106 76-77 1.350622992636623 1106 78-79 1.3243137574263422 1106 80-81 1.5481342958994944 1106 82-83 1.3221813889807947 1106 84-85 1.6060224122737914 1106 86-87 1.641680274091371 1106 88-89 1.3327840278951477 1106 90-91 1.5104965324858775 1106 92-93 1.4261732933335942 1106 94-95 1.3209750432011411 1106 96-97 1.3709101087091469 1106 98-99 1.2552919957179185 1106 100-101 1.0390831546052794 1106 102-103 0.9349103760370561 1106 104-105 1.0657091184978817 1106 106-107 1.0732841766002714 1106 108-109 0.9917733965488438 1106 110-111 0.958111678225503 1106 112-113 0.7265862003156975 1106 114-115 0.8415037910186598 1106 116-117 1.0337482975232177 1106 118-119 0.7796408220795996 1106 120-121 0.7286480067007872 1106 122-123 0.9533175857467597 1106 124-125 0.9140159509216126 1107 1 0.32068591726030604 1107 2 0.3855243168649345 1107 3 0.26843101043479933 1107 4 0.2855946807959313 1107 5 0.4217858331757327 1107 6 0.5953729493303932 1107 7 0.7683337505807373 1107 8 0.7990566885163801 1107 9 1.469975014051382 1107 10-11 0.8192142786012369 1107 12-13 1.1602119083076872 1107 14-15 1.0370933788844 1107 16-17 1.0666169722728256 1107 18-19 1.1059927682656365 1107 20-21 1.172745695831928 1107 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2216 114-115 -4.930562414981811 2216 116-117 -4.867376791066434 2216 118-119 -5.099006118624388 2216 120-121 -5.032833212292832 2216 122-123 -5.13880384113725 2216 124-125 -4.985211842605434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 118921.0 3 40411.0 4 19417.0 5 16377.0 6 17493.0 7 21158.0 8 23559.0 9 25141.0 10 27302.0 11 29524.0 12 32992.0 13 37041.0 14 39180.0 15 31392.0 16 20839.0 17 18055.0 18 15677.0 19 14671.0 20 14397.0 21 15157.0 22 18233.0 23 23792.0 24 33082.0 25 43424.0 26 53649.0 27 61006.0 28 68524.0 29 77665.0 30 87085.0 31 98931.0 32 113854.0 33 135341.0 34 162474.0 35 202700.0 36 275496.0 37 358768.0 38 130929.0 39 406.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.39384015968723 21.888619509016515 14.820960653661079 22.89657967763518 2 15.442434365612833 22.071125148471232 39.09113815926575 23.39530232665018 3 17.80704364352237 27.796453575049433 30.669955543898865 23.72654723752933 4 12.175299804165784 18.732491412903784 38.30472026348315 30.78748851944728 5 11.91899726750085 40.01346242150057 34.71379280152674 13.35374750947183 6 28.86620320681886 39.826565068407675 17.69209832258279 13.615133402190677 7 25.966432382430504 34.27566220900415 22.330178277436186 17.427727131129153 8 25.363392276658704 36.26399182587756 20.168949824152566 18.203666073311165 9 25.773564289005463 16.071151948267534 21.33302536426389 36.82225839846311 10-11 23.70388653772554 28.108105002840677 27.944280730224406 20.243727729209375 12-13 24.984231678636856 25.834492846411795 28.474418870005643 20.706856604945706 14-15 22.395660310417597 26.737548738605373 26.890101093420636 23.976689857556394 16-17 20.613445474704996 30.106574715813306 27.733237927587513 21.546741881894185 18-19 20.480106179613912 27.89439486534404 31.026416140886088 20.59908281415596 20-21 21.333472267234868 27.04583471672936 31.312445968315146 20.308247047720627 22-23 21.168818459765955 26.93400881689084 31.04536851743192 20.851804205911286 24-25 20.939592209975967 27.366413544505306 31.02633733051341 20.667656915005313 26-27 20.601428272295287 27.43884259586677 31.24710647976185 20.712622652076092 28-29 20.528942279863095 26.953952298759837 31.724710234488736 20.792395186888328 30-31 20.757285488372517 26.845002350397166 31.423175886767314 20.974536274463 32-33 20.610476529687674 27.1081206040583 31.29807480881009 20.983328057443938 34-35 20.911561343120848 27.132243172828236 31.15196241569045 20.804233068360464 36-37 21.216600575349055 26.768092968673546 30.824141364237313 21.191165091740082 38-39 20.805476284712608 27.30442420446212 30.899424614517873 20.9906748963074 40-41 20.857960033628867 27.05784656393645 31.147925810472334 20.936267591962352 42-43 21.012217103231382 27.091232777881995 30.580435717530225 21.316114401356394 44-45 20.96071945105368 27.23642357645128 30.34454150902394 21.458315463471095 46-47 21.411722150710425 27.489313839335765 29.595940506937435 21.503023503016372 48-49 21.368072304073294 27.689052866163177 29.07309149436672 21.869783335396807 50-51 21.472767592612776 27.641720884421556 28.759019502537647 22.12649202042802 52-53 21.551392160731393 28.05660082834732 28.604012104298548 21.78799490662274 54-55 21.566922607846614 27.8008419754644 28.502508033125164 22.129727383563825 56-57 21.42601177035273 28.101088190087886 28.4476933630765 22.025206676482885 58-59 21.307827894945568 27.635918754801285 28.809166015269827 22.247087334983316 60-61 21.586248045314242 27.955443267462027 28.69714424719193 21.761164440031806 62-63 21.489450170371335 27.815762433759613 29.026715247407388 21.66807214846167 64-65 21.408213970349948 27.587511657154295 28.721246099003306 22.28302827349245 66-67 21.292749365185948 27.95572038214894 28.378744768680342 22.37278548398477 68-69 21.3165801412154 27.67937531311701 28.691789455417588 22.312255090250005 70-71 21.455570886205088 27.60826349523622 28.863140441185937 22.073025177372756 72-73 21.53416247818866 27.66023034679686 28.398891918434604 22.40671525657988 74-75 21.296554932056274 27.984327597430514 28.260028915257852 22.459088555255363 76-77 21.320936667496035 27.760882277897252 28.66127419362507 22.256906860981644 78-79 21.332718177575313 27.644984869387034 28.456883497845897 22.565413455191756 80-81 21.23721306149616 28.009575842095668 28.217098642663014 22.536112453745158 82-83 21.45928723592655 27.959922521682323 28.135671047569772 22.445119194821363 84-85 21.17112370016121 27.612306031980978 28.15298191843865 23.06358834941917 86-87 21.165049889958816 27.679547394965642 28.508035680680493 22.64736703439505 88-89 21.40586535278665 27.531635476741418 28.337174089564463 22.725325080907464 90-91 21.25694691374288 27.72089724758102 28.41690099552208 22.605254843154015 92-93 21.41619875784272 27.644338580568274 28.27912564045268 22.660337021136325 94-95 21.14940333674993 27.245297356963462 28.457126578822983 23.148172727463624 96-97 21.440556387349112 27.31657367263115 28.21760485452241 23.025265085497328 98-99 21.054525976570314 27.2091465228879 28.27488854279786 23.461438957743923 100-101 21.04071452526926 27.354279899311635 28.33832304784559 23.26668252757351 102-103 21.294711890273497 27.11344319242986 28.32317956150127 23.268665355795378 104-105 21.148734190379653 27.16420967597199 28.37632596576186 23.310730167886497 106-107 20.89565927943543 27.17712021790269 28.31726631174941 23.609954190912468 108-109 21.1962221228234 27.224261042612646 27.806120528687277 23.773396305876677 110-111 20.88811368203775 27.14530077068371 28.08464149489463 23.88194405238391 112-113 20.91061208715084 27.021816632366775 28.40922394689034 23.658347333592044 114-115 20.910089661137082 27.304096298858305 27.93001272949642 23.85580131050819 116-117 20.730484936390255 27.300626014485374 27.99393279803238 23.97495625109199 118-119 20.871502930616845 26.89275772748631 28.004942073071685 24.230797268825164 120-121 20.614109287768567 27.109144741455715 28.079233057725506 24.197512913050215 122-123 20.707959132249677 27.237441185652866 27.946660200852286 24.10793948124518 124-125 20.58109341479104 27.089811955605047 27.845127603888592 24.483967025715323 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 449.0 1 343.5 2 315.0 3 450.5 4 396.5 5 341.5 6 643.0 7 1510.0 8 2388.5 9 2817.5 10 2515.5 11 1910.5 12 1774.0 13 1877.0 14 2131.0 15 2422.5 16 2727.5 17 3080.0 18 3539.0 19 4016.5 20 4492.5 21 5233.0 22 6294.5 23 7539.5 24 8874.5 25 11149.0 26 15633.5 27 20489.0 28 23673.0 29 28535.0 30 36135.5 31 43039.0 32 51126.0 33 60674.0 34 70468.5 35 79411.0 36 90070.5 37 99357.0 38 100716.0 39 100139.5 40 96122.0 41 90577.0 42 86990.0 43 85951.5 44 87794.5 45 89157.0 46 87051.0 47 83234.0 48 81715.5 49 79556.0 50 76397.5 51 72165.0 52 68132.0 53 66319.0 54 63993.5 55 59318.0 56 53771.0 57 48319.0 58 44877.5 59 41265.5 60 35422.0 61 29307.5 62 23550.5 63 18144.5 64 13619.0 65 10412.0 66 7775.5 67 5764.5 68 4272.0 69 3111.5 70 2369.0 71 1912.0 72 1603.0 73 1459.0 74 1213.0 75 887.5 76 652.5 77 452.5 78 307.5 79 199.5 80 135.5 81 98.5 82 69.0 83 46.5 84 32.0 85 22.5 86 14.5 87 5.0 88 4.0 89 3.5 90 5.0 91 4.0 92 1.0 93 2.5 94 2.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10435555689378594 2 1.9809331225092243E-4 3 0.0 4 0.18165156733409585 5 0.0 6 1.5847464980073793E-4 7 0.03633031346681918 8 0.0 9 0.0 10-11 6.735172616531362E-4 12-13 5.942799367527672E-4 14-15 4.556146181771216E-4 16-17 0.004199578219719555 18-19 0.0015055091731070104 20-21 0.0032091116584649433 22-23 0.0017432211478081174 24-25 0.004635383506671585 26-27 0.004140150226044279 28-29 0.0032685396521402203 30-31 8.518012426789665E-4 32-33 7.923732490036897E-5 34-35 0.0012281785359557192 36-37 0.0011885598735055345 38-39 6.338985992029517E-4 40-41 0.001624365160457564 42-43 7.725639177785975E-4 44-45 0.0010895132173800732 46-47 1.5847464980073793E-4 48-49 1.980933122509224E-5 50-51 0.04191654487229519 52-53 3.961866245018448E-5 54-55 0.14112167564755712 56-57 3.763772932767526E-4 58-59 0.0 60-61 0.0 62-63 6.933265928782285E-4 64-65 0.058259243132996276 66-67 0.050593031948885585 68-69 0.0018422678039335784 70-71 0.0027931157027380063 72-73 0.07392842413204424 74-75 0.00538813809322509 76-77 0.002020551784959409 78-79 0.001624365160457564 80-81 1.1885598735055346E-4 82-83 7.923732490036897E-5 84-85 0.0 86-87 3.1694929960147586E-4 88-89 0.0014856998418819182 90-91 4.1599595572693705E-4 92-93 0.0026544503841623604 94-95 0.004358052869520293 96-97 0.002614831721712176 98-99 0.0 100-101 0.0014856998418819182 102-103 0.0016639838229077482 104-105 2.5752130592619917E-4 106-107 1.980933122509224E-5 108-109 0.0 110-111 2.1790264347601468E-4 112-113 9.904665612546121E-5 114-115 1.5847464980073793E-4 116-117 0.0 118-119 8.319919114538741E-4 120-121 0.002614831721712176 122-123 7.527545865535052E-4 124-125 0.008617059082915126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2524063.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.376344421533084 #Duplication Level Percentage of deduplicated Percentage of total 1 76.06037205125715 34.51341639027793 2 10.745679503053555 9.751993083479332 3 4.551861840888219 6.196405519541324 4 2.4578573094974163 4.461143192589496 5 1.4799331225346883 3.3576977544484468 6 0.966069713505479 2.6302027233141843 7 0.6612016093124878 2.1002038369364784 8 0.48305364537746776 1.753536686938006 9 0.3866289274681368 1.57894266385099 >10 1.9364539237219753 16.061623973283933 >50 0.15376459236640821 4.906162976830062 >100 0.10951985535152131 9.336919976500543 >500 0.006517633427643921 1.9907428151654532 >1k 9.052268649505119E-4 0.6883496976486478 >5k 1.8104537299010236E-4 0.6726587091952654 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8516 0.337392529425771 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7860 0.3114026868584501 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4723 0.18711894275222132 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.961866245018448E-5 0.0 2 0.0 0.0 0.0 3.961866245018448E-5 0.0 3 0.0 0.0 0.0 3.961866245018448E-5 0.0 4 0.0 0.0 0.0 3.961866245018448E-5 0.0 5 1.1885598735055345E-4 0.0 0.0 3.961866245018448E-5 0.0 6 2.377119747011069E-4 0.0 0.0 3.961866245018448E-5 0.0 7 3.9618662450184485E-4 0.0 0.0 3.961866245018448E-5 0.0 8 4.754239494022138E-4 0.0 0.0 3.961866245018448E-5 0.0 9 4.754239494022138E-4 0.0 0.0 3.961866245018448E-5 0.0 10-11 5.150426118523983E-4 0.0 0.0 5.9427993675276724E-5 0.0 12-13 6.735172616531362E-4 0.0 0.0 7.923732490036897E-5 0.0 14-15 8.716105739040587E-4 0.0 0.0 7.923732490036897E-5 0.0 16-17 9.112292363542432E-4 0.0 0.0 7.923732490036897E-5 0.0 18-19 0.0011093225486051655 0.0 0.0 1.5847464980073793E-4 0.0 20-21 0.0012677971984059034 0.0 0.0 3.9618662450184485E-4 0.0 22-23 0.0012876065296309957 0.0 0.0 6.933265928782285E-4 0.0 24-25 0.0016639838229077484 0.0 0.0 0.0011687505422804422 0.0 26-27 0.0016837931541328407 0.0 0.0 0.0020007424537343164 0.0 28-29 0.0019215051288339474 0.0 0.0 0.003526060958066419 0.0 30-31 0.0021394077723099624 0.0 0.0 0.0086764870765904 0.0 32-33 0.0021592171035350544 0.0 0.0 0.019413144600590397 0.0 34-35 0.002377119747011069 0.0 0.0 0.034210715025734306 0.0 36-37 0.0026544503841623604 0.0 0.0 0.059051616381999975 0.0 38-39 0.0030506370086642053 0.0 0.0 0.0996013173997638 0.0 40-41 0.0030506370086642053 0.0 0.0 0.14785684826408851 0.0 42-43 0.003169492996014759 0.0 0.0 0.19407201801222868 0.0 44-45 0.003407204970715866 0.0 0.0 0.2500135693918892 0.0 46-47 0.003684535607867157 0.0 0.0 0.3178605288378301 0.0 48-49 0.0038033915952177103 0.0 0.0 0.39394817007340943 3.961866245018448E-5 50-51 0.0038033915952177103 0.0 0.0 0.47791992513657544 3.961866245018448E-5 52-53 0.0038232009264428024 0.0 0.0 0.557711911311247 3.961866245018448E-5 54-55 0.003843010257667895 0.0 0.0 0.6445956380645015 3.961866245018448E-5 56-57 0.004001484907468633 0.0 0.0 0.7467325498610772 5.9427993675276724E-5 58-59 0.004001484907468633 0.0 0.0 0.8649150199499775 7.923732490036897E-5 60-61 0.004041103569918818 0.0 0.0 0.9941709061937044 7.923732490036897E-5 62-63 0.004041103569918818 3.961866245018448E-5 0.0 1.125130394922789 7.923732490036897E-5 64-65 0.004120340894819187 3.961866245018448E-5 0.0 1.2581896727617337 7.923732490036897E-5 66-67 0.004318434207070109 3.961866245018448E-5 0.0 1.415891758644693 7.923732490036897E-5 68-69 0.004397671531970478 3.961866245018448E-5 0.0 1.6020994721605601 7.923732490036897E-5 70-71 0.004536336850546123 3.961866245018448E-5 0.0 1.8115633405346856 7.923732490036897E-5 72-73 0.004655192837896676 3.961866245018448E-5 0.0 2.027920856175143 7.923732490036897E-5 74-75 0.004675002169121769 3.961866245018448E-5 0.0 2.239464704327903 7.923732490036897E-5 76-77 0.004714620831571953 3.961866245018448E-5 0.0 2.4683219079713936 7.923732490036897E-5 78-79 0.00477404882524723 3.961866245018448E-5 0.0 2.7391550844808545 7.923732490036897E-5 80-81 0.005586231405476013 3.961866245018448E-5 0.0 3.0431688907923453 7.923732490036897E-5 82-83 0.005625850067926197 3.961866245018448E-5 0.0 3.360653042336899 7.923732490036897E-5 84-85 0.005744706055276751 3.961866245018448E-5 0.0 3.680593550953364 1.1885598735055345E-4 86-87 0.005883371373852395 3.961866245018448E-5 0.0 4.011864997030582 1.1885598735055345E-4 88-89 0.005903180705077488 3.961866245018448E-5 0.0 4.380477032467097 1.1885598735055345E-4 90-91 0.006319176660804425 3.961866245018448E-5 0.0 4.805902229857178 1.1885598735055345E-4 92-93 0.006438032648154979 3.961866245018448E-5 0.0 5.263200641188433 1.1885598735055345E-4 94-95 0.006457841979380071 3.961866245018448E-5 0.0 5.724817486726757 1.1885598735055345E-4 96-97 0.006457841979380071 7.923732490036897E-5 0.0 6.188236981406566 1.3866531857564568E-4 98-99 0.00671536328530627 7.923732490036897E-5 0.0 6.688759353470971 1.5847464980073793E-4 100-101 0.007052121916132838 7.923732490036897E-5 0.0 7.253959191985303 1.5847464980073793E-4 102-103 0.007388880546959406 7.923732490036897E-5 0.0 7.872980983438211 1.5847464980073793E-4 104-105 0.008379347108214018 7.923732490036897E-5 1.980933122509224E-5 8.487862624665073 1.5847464980073793E-4 106-107 0.0086764870765904 7.923732490036897E-5 3.961866245018448E-5 9.112946071472859 1.5847464980073793E-4 108-109 0.008716105739040586 7.923732490036897E-5 3.961866245018448E-5 9.746329628063958 1.5847464980073793E-4 110-111 0.008795343063940956 7.923732490036897E-5 3.961866245018448E-5 10.45603853786534 1.5847464980073793E-4 112-113 0.008953817713741694 7.923732490036897E-5 3.961866245018448E-5 11.20966473499275 1.5847464980073793E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7020 0.0 67.45158 1 GTATCAA 12655 0.0 48.30259 1 TCAACGC 15835 0.0 36.05407 4 ATCAACG 16115 0.0 35.945354 3 TATCAAC 16810 0.0 35.558228 2 CAACGCA 16160 0.0 35.196526 5 AACGCAG 16490 0.0 34.023132 6 GTACATG 12070 0.0 32.708412 1 TACATGG 13055 0.0 29.626112 2 ACGCAGA 19180 0.0 28.941183 7 CGCAGAG 19450 0.0 28.895044 8 ACATGGG 12705 0.0 27.909311 3 GAGTACT 12615 0.0 26.872454 12-13 GCAGAGT 20295 0.0 26.695648 9 GTACTTT 13520 0.0 25.337605 14-15 TATAACG 195 1.4458274E-6 24.449062 2 CAGAGTA 19720 0.0 24.368242 10-11 AGAGTAC 19115 0.0 24.29945 10-11 AGTACTT 13855 0.0 24.059626 12-13 CATGGGA 8390 0.0 23.724188 4 >>END_MODULE