FastQCFastQC Report
Thu 26 May 2016
SRR1364482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254746
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57500.053.007991
GGTATCA30500.048.0147321
ATCAACG64850.045.0504043
TCAACGC66450.044.809344
CAACGCA67800.044.3553055
AACGCAG66850.043.7142376
TATCAAC72800.041.5202032
ACGCAGA80400.036.052477
GTACATG94600.035.365881
CGCAGAG81100.034.8644688
TACATGG96200.033.3999522
ACATGGG97000.030.4254593
GCAGAGT85800.030.3189479
CATGGGA54700.024.9311144
CAGAGTA86300.023.1006310-11
AGAGTAC82500.022.75805910-11
GAGTACT36750.022.74260112-13
GAGTACA75000.021.748461
CATGGGG50350.021.6443794
GTGTAGC12600.021.2608781
Produced by FastQC (version 0.11.5)