FastQCFastQC Report
Thu 26 May 2016
SRR1364482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254746
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40630.18019768080307053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38760.1719040636949794No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39400.064.1744461
GTATCAA69850.050.2853361
ATCAACG86100.039.4393543
TCAACGC87450.038.6261444
CAACGCA88650.038.1129155
TATCAAC91500.038.0233042
AACGCAG91000.037.2594156
CGCAGAG111800.031.2243168
ACGCAGA109800.030.934027
GTACATG90300.030.5763571
TACATGG91800.029.5275122
GCAGAGT121700.027.3160469
ACATGGG92600.027.0850243
GAGTACT69450.025.99149312-13
CAGAGTA118300.024.25813110-11
GTACTTT77750.023.48457714-15
AGTACTT74400.022.74333412-13
AGAGTAC114000.022.66886110-11
CATGGGA52250.021.4353054
CATGGGG44100.021.3440424
Produced by FastQC (version 0.11.5)