##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364482_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2254746 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.12601508107787 31.0 28.0 31.0 25.0 34.0 2 29.28195548412105 31.0 28.0 33.0 25.0 34.0 3 29.382757969190322 31.0 28.0 34.0 26.0 34.0 4 32.84035718435691 35.0 32.0 37.0 27.0 37.0 5 32.03302411890297 35.0 32.0 37.0 22.0 37.0 6 32.088315490968824 35.0 32.0 37.0 23.0 37.0 7 31.57632700091274 35.0 32.0 37.0 18.0 37.0 8 31.672996869713927 35.0 32.0 37.0 19.0 37.0 9 32.25160616761267 37.0 31.0 39.0 16.0 39.0 10-11 32.45460552984682 37.0 31.5 39.0 17.0 39.0 12-13 32.22983542270393 37.0 31.0 39.0 16.0 39.0 14-15 33.20660841620298 38.0 32.0 40.0 17.0 41.0 16-17 33.26159288008494 38.0 32.0 40.0 17.0 41.0 18-19 33.094758123531435 38.0 31.5 40.0 16.5 41.0 20-21 32.98963830072212 38.0 31.0 40.0 14.0 41.0 22-23 32.975312296817464 38.0 31.0 40.0 15.5 41.0 24-25 32.782882417797836 38.0 31.0 40.0 11.5 41.0 26-27 32.86095595690158 38.0 31.0 40.0 14.0 41.0 28-29 32.73659361187468 37.5 31.0 40.0 10.5 41.0 30-31 32.241601049519545 37.0 30.0 40.0 9.0 41.0 32-33 32.184201235970704 37.0 30.0 40.0 9.0 41.0 34-35 31.988200888259698 37.0 30.0 40.0 8.0 41.0 36-37 31.96700382216001 37.0 30.0 40.0 8.0 41.0 38-39 31.737760040377054 37.0 30.0 40.0 7.5 41.0 40-41 31.46007709959348 36.5 30.0 40.0 2.0 41.0 42-43 31.34632792341133 36.5 30.0 40.0 2.0 41.0 44-45 31.271423255657176 36.0 30.0 40.0 2.0 41.0 46-47 31.119016066554725 36.0 29.5 40.0 2.0 41.0 48-49 30.851887751436305 36.0 29.0 40.0 2.0 41.0 50-51 30.50349839848923 36.0 28.0 40.0 2.0 41.0 52-53 30.406182780676847 36.0 28.0 40.0 2.0 41.0 54-55 29.927143678267974 35.0 27.0 40.0 2.0 41.0 56-57 29.940564702188183 35.0 27.5 39.0 2.0 41.0 58-59 29.702781821100913 35.0 27.0 39.0 2.0 41.0 60-61 29.41373152452649 35.0 26.5 39.0 2.0 41.0 62-63 29.139290855821454 34.5 26.0 39.0 2.0 40.5 64-65 28.659616426861383 34.0 26.0 38.0 2.0 40.0 66-67 28.26882007995579 34.0 25.0 38.0 2.0 40.0 68-69 28.08165864359001 34.0 24.5 37.5 2.0 40.0 70-71 28.158832081307608 34.0 26.0 37.0 2.0 40.0 72-73 27.929153217258175 34.0 25.5 37.0 2.0 39.0 74-75 27.568678911061376 34.0 24.5 36.0 2.0 39.0 76-77 27.48920987108969 34.0 25.5 36.0 2.0 39.0 78-79 27.0836411285351 33.0 24.0 35.5 2.0 37.5 80-81 26.85375891563839 33.0 24.0 35.0 2.0 37.0 82-83 26.702485778885958 33.0 24.0 35.0 2.0 37.0 84-85 26.27978229033337 33.0 22.5 35.0 2.0 36.0 86-87 26.15513853888642 33.0 22.5 35.0 2.0 36.0 88-89 26.119754287179134 33.0 23.0 35.0 2.0 36.0 90-91 25.956476028785502 33.0 23.0 35.0 2.0 35.0 92-93 25.97641308599727 33.0 23.0 35.0 2.0 35.0 94-95 25.989054199453065 33.0 23.0 35.0 2.0 35.0 96-97 25.869779567188502 33.0 23.0 35.0 2.0 35.0 98-99 25.73704953906116 33.0 21.5 35.0 2.0 35.0 100-101 25.64061184718811 33.0 20.0 35.0 2.0 35.0 102-103 24.4869796864037 30.5 19.0 34.0 2.0 35.0 104-105 25.452997809952873 32.5 20.0 35.0 2.0 35.0 106-107 25.564744099778867 33.0 20.0 35.0 2.0 35.0 108-109 25.635339634708302 33.0 21.0 35.0 2.0 35.0 110-111 25.56737366426196 33.0 20.0 35.0 2.0 35.0 112-113 25.515660744048333 33.0 20.0 35.0 2.0 35.0 114-115 25.46461796583739 33.0 19.5 35.0 2.0 35.0 116-117 25.378287620867273 33.0 19.0 35.0 2.0 35.0 118-119 25.250906975774654 33.0 18.5 35.0 2.0 35.0 120-121 25.08521558525883 33.0 16.0 35.0 2.0 35.0 122-123 24.788088990955078 33.0 2.0 35.0 2.0 35.0 124-125 23.991157540583284 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9591141591047965 1101 2 0.9470839022512756 1101 3 0.959110452221438 1101 4 0.9954152505219298 1101 5 1.2499808248451174 1101 6 1.318050473715175 1101 7 1.6713782807454614 1101 8 1.4844273543983135 1101 9 2.1696507498586612 1101 10-11 1.8824649042462909 1101 12-13 2.0883740661887487 1101 14-15 2.0487388794769146 1101 16-17 1.9419863232102585 1101 18-19 2.065729046964236 1101 20-21 2.2191264315818913 1101 22-23 2.2827376859251345 1101 24-25 2.3886169131215382 1101 26-27 2.156981156591179 1101 28-29 2.175292439387107 1101 30-31 2.6358593187591737 1101 32-33 2.5156645643096667 1101 34-35 2.583907165047055 1101 36-37 2.454274466346739 1101 38-39 2.50457499044267 1101 40-41 2.568966194298035 1101 42-43 2.624137509076114 1101 44-45 2.466394507334286 1101 46-47 2.3604790344813544 1101 48-49 2.440288933859101 1101 50-51 2.5997826669364663 1101 52-53 2.514145181420524 1101 54-55 2.8299174834238343 1101 56-57 2.5679799685398024 1101 58-59 2.6800851097707437 1101 60-61 2.713383748583503 1101 62-63 2.734531887610224 1101 64-65 2.9238140499634326 1101 66-67 3.146999536453926 1101 68-69 2.990895404052477 1101 70-71 2.523117456377271 1101 72-73 2.7507022781910884 1101 74-75 2.8305614716496876 1101 76-77 2.5985431588592753 1101 78-79 2.6856778968910575 1101 80-81 2.692604425336686 1101 82-83 2.6227126336430686 1101 84-85 2.90964156424387 1101 86-87 2.747314544654124 1101 88-89 2.5666735887028125 1101 90-91 2.615135904250483 1101 92-93 2.5477121131366545 1101 94-95 2.4757306147375964 1101 96-97 2.418316336772243 1101 98-99 2.5485687944055186 1101 100-101 2.5516451315045963 1101 102-103 2.3019377570622375 1101 104-105 2.374494207607114 1101 106-107 2.5309877033268577 1101 108-109 2.465527374216574 1101 110-111 2.375669060183945 1101 112-113 2.337117114822785 1101 114-115 2.4433402670840856 1101 116-117 2.5128521691430805 1101 118-119 2.385525773049519 1101 120-121 2.4148694213103745 1101 122-123 2.4429565612769117 1101 124-125 2.1921652794972104 1102 1 0.4442310259445996 1102 2 -1.7787465063863657 1102 3 -0.020865666261702387 1102 4 0.44050642843478016 1102 5 0.7985234152785878 1102 6 0.37680641261285786 1102 7 1.1348767451876292 1102 8 0.8227210407593333 1102 9 1.6272783907885042 1102 10-11 1.2595667221749096 1102 12-13 1.3900046611970325 1102 14-15 1.459701784607148 1102 16-17 1.3983996660448383 1102 18-19 1.4494076894511068 1102 20-21 1.7093433059772494 1102 22-23 1.6556157198185133 1102 24-25 1.8080558308088257 1102 26-27 1.5083963438729384 1102 28-29 1.5098444165011458 1102 30-31 1.8324305729388115 1102 32-33 1.436195187317999 1102 34-35 1.7462866648685313 1102 36-37 1.2536947617842102 1102 38-39 1.1735398333535514 1102 40-41 1.4434148971850007 1102 42-43 1.2709775043946223 1102 44-45 1.231557640909294 1102 46-47 1.040590338349947 1102 48-49 1.1059371051897244 1102 50-51 1.1718104773480356 1102 52-53 1.041553740544316 1102 54-55 1.408419599723267 1102 56-57 0.9919920781873799 1102 58-59 0.9789710340461824 1102 60-61 1.1848705211564976 1102 62-63 1.4030334705755294 1102 64-65 1.5436319311751063 1102 66-67 1.5747139478826853 1102 68-69 1.7059032040640325 1102 70-71 1.0957769714284034 1102 72-73 1.2753920184721856 1102 74-75 1.4516535028971447 1102 76-77 1.330546613679207 1102 78-79 1.2007817711190185 1102 80-81 1.3108723783262448 1102 82-83 1.0223249293649879 1102 84-85 1.135581306376821 1102 86-87 1.1061100391659657 1102 88-89 1.223341154136243 1102 90-91 1.0731866569182635 1102 92-93 1.1095987233390403 1102 94-95 1.1169726758369478 1102 96-97 1.1613941042948603 1102 98-99 0.9321826960557438 1102 100-101 0.7562034345539352 1102 102-103 0.5317932827741281 1102 104-105 0.8924296415855366 1102 106-107 1.0710922812595634 1102 108-109 0.9588410680146389 1102 110-111 0.9155474896334681 1102 112-113 0.9498746892784489 1102 114-115 1.1442056016993973 1102 116-117 0.7233406291259854 1102 118-119 0.347140234700575 1102 120-121 0.6527152551560533 1102 122-123 1.0463842700587023 1102 124-125 1.0313993376217425 1103 1 0.5735967312299408 1103 2 0.5882133621273091 1103 3 0.38494601374236126 1103 4 0.47144910720574984 1103 5 0.9525147101241132 1103 6 0.8913528913134172 1103 7 1.069612652424798 1103 8 0.9606702735629398 1103 9 1.7189558165701442 1103 10-11 1.3823194218951542 1103 12-13 1.2320559452516449 1103 14-15 1.078456570992195 1103 16-17 1.1558117359162594 1103 18-19 1.1868004056867818 1103 20-21 1.3299786524382924 1103 22-23 1.2413464213618894 1103 24-25 1.5399751837985036 1103 26-27 1.0634290854887567 1103 28-29 1.2218224328984775 1103 30-31 1.8677836749971277 1103 32-33 1.5688240484103986 1103 34-35 1.68738868020991 1103 36-37 1.631680538266771 1103 38-39 1.650158271939901 1103 40-41 1.7702965052511175 1103 42-43 1.657995040161886 1103 44-45 1.6491022653809964 1103 46-47 1.652559407390637 1103 48-49 1.7070071895112378 1103 50-51 1.8181406262437712 1103 52-53 1.6417440765877984 1103 54-55 1.9445729680837616 1103 56-57 1.71847953678801 1103 58-59 1.7373226890517373 1103 60-61 1.7126174685753384 1103 62-63 1.7156931763840362 1103 64-65 1.9685239431163382 1103 66-67 2.2836434022952616 1103 68-69 2.2647793274230636 1103 70-71 1.9479364771797272 1103 72-73 1.968174987453029 1103 74-75 2.0513179657544924 1103 76-77 1.8201488236687844 1103 78-79 1.9995125235969695 1103 80-81 2.0274914885277795 1103 82-83 2.0135684271308527 1103 84-85 2.1545305832035275 1103 86-87 1.9996097131997175 1103 88-89 1.8705951870952724 1103 90-91 1.9337557388503583 1103 92-93 1.776458698484774 1103 94-95 1.5809057760532 1103 96-97 1.4492812014252934 1103 98-99 1.6726808437050096 1103 100-101 1.680453355334894 1103 102-103 1.6025600374318962 1103 104-105 1.5894076136592616 1103 106-107 1.5625517250930763 1103 108-109 1.5495604655382564 1103 110-111 1.622127530462265 1103 112-113 1.5299417741695542 1103 114-115 1.4044646363134206 1103 116-117 1.5448628757403746 1103 118-119 1.628785855671918 1103 120-121 1.5457335674126398 1103 122-123 1.6181342573250568 1103 124-125 1.4849629322925146 1104 1 0.5092476298640989 1104 2 0.391197216889811 1104 3 0.39467995320509175 1104 4 0.42711332716686456 1104 5 0.6948622457996763 1104 6 0.7760409559370913 1104 7 1.0788685109105494 1104 8 0.9395220617666595 1104 9 1.3636675246265781 1104 10-11 0.8071117087414663 1104 12-13 1.0187367331325135 1104 14-15 0.9917297338161504 1104 16-17 0.7587676207342042 1104 18-19 0.9082722371470737 1104 20-21 1.315259219892063 1104 22-23 1.253550979921684 1104 24-25 1.365087373325352 1104 26-27 1.2917109362275099 1104 28-29 1.2554647648114212 1104 30-31 1.6936727995164986 1104 32-33 1.6254581304714293 1104 34-35 1.4704447327478256 1104 36-37 1.4427891705977274 1104 38-39 1.5126504979615447 1104 40-41 1.668647296776335 1104 42-43 1.6854422928197792 1104 44-45 1.3706763138866336 1104 46-47 1.3409415412040424 1104 48-49 1.1765232610499226 1104 50-51 1.6270835104492 1104 52-53 1.3784511966618886 1104 54-55 1.6962050584967514 1104 56-57 1.6536178548572664 1104 58-59 1.6693316915572112 1104 60-61 1.690174721157753 1104 62-63 1.7525221451575348 1104 64-65 1.810172650296792 1104 66-67 1.8256670334804994 1104 68-69 1.9547543168239656 1104 70-71 1.7010676797178768 1104 72-73 1.8919544319078376 1104 74-75 1.9131648465431432 1104 76-77 1.7959007046261917 1104 78-79 1.8544378756031392 1104 80-81 1.8353019388908791 1104 82-83 1.8010822963924404 1104 84-85 1.9552489650254579 1104 86-87 1.9543243222122193 1104 88-89 1.8204148421905373 1104 90-91 1.8886045010592625 1104 92-93 1.567209796881869 1104 94-95 1.5452867138808841 1104 96-97 1.6343781840749543 1104 98-99 1.7350806649480077 1104 100-101 1.6943866672397974 1104 102-103 1.5904122305162254 1104 104-105 1.4268414253631505 1104 106-107 1.5826287407056903 1104 108-109 1.504807165718539 1104 110-111 1.5769653624611664 1104 112-113 1.5898044337666448 1104 114-115 1.401746467541578 1104 116-117 1.4439399031732805 1104 118-119 1.4604427487607943 1104 120-121 1.3574184389908623 1104 122-123 1.5829796044663915 1104 124-125 1.411091517419198 1105 1 0.6798456411212577 1105 2 0.7556806776758549 1105 3 0.5192803191397601 1105 4 0.7261553564194756 1105 5 0.9208433770146058 1105 6 1.0130074648723095 1105 7 1.3824800475354557 1105 8 1.25771386626311 1105 9 1.9649372174984236 1105 10-11 1.6078625193758143 1105 12-13 1.755155325617764 1105 14-15 1.6144629466675795 1105 16-17 1.591830322979149 1105 18-19 1.66942037479226 1105 20-21 1.8818587467408676 1105 22-23 1.803919835275913 1105 24-25 1.9882968522907305 1105 26-27 1.782966393990428 1105 28-29 1.770699741225087 1105 30-31 2.1282173760248853 1105 32-33 2.052448020724121 1105 34-35 2.1221002943317977 1105 36-37 1.8772416663530151 1105 38-39 1.9545669300740798 1105 40-41 2.1302897282204185 1105 42-43 2.136289335034924 1105 44-45 2.023918362102137 1105 46-47 1.850570166577949 1105 48-49 1.8924334555546949 1105 50-51 1.9708102070046998 1105 52-53 1.999733706023548 1105 54-55 2.1757735623327044 1105 56-57 1.9608038152472886 1105 58-59 1.8924403708731852 1105 60-61 1.6396815927558883 1105 62-63 1.613318994729667 1105 64-65 2.024011243671211 1105 66-67 2.254611841284227 1105 68-69 2.1988066914930684 1105 70-71 2.2101997884059656 1105 72-73 2.3517484141716487 1105 74-75 2.2488336139458696 1105 76-77 2.0394388298706936 1105 78-79 2.2219309382535855 1105 80-81 2.2147549245445575 1105 82-83 2.0486470122564135 1105 84-85 2.3093756790763003 1105 86-87 2.271419790242142 1105 88-89 2.051267620748842 1105 90-91 2.180408983118692 1105 92-93 1.9963196666293257 1105 94-95 1.8202760634469044 1105 96-97 1.8275096864688365 1105 98-99 1.8743448188748033 1105 100-101 1.8675536955392325 1105 102-103 1.7677302303899047 1105 104-105 1.4906370573111971 1105 106-107 1.6548987154309636 1105 108-109 1.598260245754986 1105 110-111 1.7045029245743066 1105 112-113 1.8114641001379361 1105 114-115 1.7722495636849658 1105 116-117 1.6107842209491352 1105 118-119 1.7486260832667178 1105 120-121 1.8227924069850303 1105 122-123 1.8861954456363001 1105 124-125 1.7338864963132785 1106 1 0.4962880164765906 1106 2 0.5295918841987621 1106 3 0.5131912387434276 1106 4 0.4361817641392989 1106 5 0.773714758477972 1106 6 0.9620446612758826 1106 7 1.226448458767738 1106 8 0.7870774117464592 1106 9 1.6979329970353305 1106 10-11 1.3383082484156787 1106 12-13 1.2142018781158583 1106 14-15 1.407514342451634 1106 16-17 1.3471151189427744 1106 18-19 1.388521941453078 1106 20-21 1.2138581286008687 1106 22-23 1.1095859030544162 1106 24-25 1.0167069116198206 1106 26-27 1.00242486291355 1106 28-29 1.1510709195778759 1106 30-31 1.5420556024834013 1106 32-33 1.300561508136063 1106 34-35 1.306803365995524 1106 36-37 1.3267376362154906 1106 38-39 1.4285525720703731 1106 40-41 1.6471216298936397 1106 42-43 1.5912906979013144 1106 44-45 1.4040150345599614 1106 46-47 1.4571929241944552 1106 48-49 1.4234317717252765 1106 50-51 1.7496810653236103 1106 52-53 1.5641089806316515 1106 54-55 2.033122667899139 1106 56-57 1.7767892382632553 1106 58-59 1.6882439985878896 1106 60-61 1.961101756771118 1106 62-63 1.912115836804336 1106 64-65 2.0492277654919775 1106 66-67 2.0002926474945895 1106 68-69 1.9050100118325268 1106 70-71 1.8852054729004983 1106 72-73 1.7138141821567068 1106 74-75 2.032129495398568 1106 76-77 1.6717788056817682 1106 78-79 1.596263544402742 1106 80-81 1.6604939991467589 1106 82-83 1.593511183870394 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-5.5079029387508776 2216 104-105 -5.265244637681057 2216 106-107 -5.561084880860928 2216 108-109 -5.593562602489122 2216 110-111 -5.311694787094591 2216 112-113 -5.396622534104807 2216 114-115 -5.1463123134133255 2216 116-117 -4.998935010653913 2216 118-119 -5.236534831478398 2216 120-121 -5.102385972112334 2216 122-123 -5.192931371299803 2216 124-125 -5.123721163817642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 98997.0 3 33506.0 4 16581.0 5 13943.0 6 14281.0 7 16506.0 8 18655.0 9 19726.0 10 21680.0 11 23015.0 12 24761.0 13 26772.0 14 27352.0 15 22403.0 16 16525.0 17 14384.0 18 12689.0 19 12005.0 20 11866.0 21 12848.0 22 15397.0 23 20998.0 24 29351.0 25 38382.0 26 47321.0 27 54793.0 28 62136.0 29 70758.0 30 78306.0 31 89614.0 32 104116.0 33 123584.0 34 150665.0 35 189704.0 36 264805.0 37 338337.0 38 117503.0 39 481.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.679320106271135 20.002823714943315 14.105210908419288 24.212645270366266 2 15.976077064317836 20.744697836557652 38.60755563834411 24.6716694607804 3 19.40577785701804 25.097904597679737 29.261477789515983 26.234839755786236 4 13.209627892862077 17.356039470930643 36.291653726069896 33.142678910137384 5 13.522853571976622 38.43102504672367 33.779680726786964 14.266440654512746 6 31.969186732146415 37.049943164254195 17.001405481689044 13.979464621910346 7 28.072535356860506 32.42130275800814 20.659329251141738 18.846832633989614 8 26.01428276178337 36.18695853102744 19.197239955187857 18.601518752001333 9 25.75451957781497 16.197035054059306 19.760718058708164 38.287727309417555 10-11 25.154414036071227 26.881181301470885 26.9056631304764 21.058741531981497 12-13 25.927404296914847 23.922223197947076 27.68346510053958 22.4669074045985 14-15 22.801589985530466 25.28139084926738 25.59071732296997 26.32630184223219 16-17 22.467221192748525 28.776470755209882 26.073377869282172 22.682930182759424 18-19 22.406051852684676 27.019385381164668 28.520440171028305 22.05412259512235 20-21 23.016255994209367 26.349905707072768 28.56984772978468 22.063990568933185 22-23 22.712706259758733 26.12864217883308 28.5330739653388 22.625577596069384 24-25 22.579328884302807 26.365595346680426 28.47774276123307 22.577333007783697 26-27 22.389382336478526 26.72804478550627 28.33023899855322 22.552333879461987 28-29 22.295334411388236 26.648749843658386 28.61940818156739 22.43650756338599 30-31 22.296995978449726 26.326507305206505 28.5414312816072 22.83506543473657 32-33 22.29189997095015 26.474922884849505 28.37826450441181 22.854912639788534 34-35 22.737410036046565 26.56468811572156 28.17701513157384 22.520886716658037 36-37 22.794268474280095 26.328494970001852 28.17914399665591 22.698092559062147 38-39 22.56241472118804 26.495291134906452 28.34521905331288 22.597075090592625 40-41 22.65381655774677 26.310708892254674 28.325863800968193 22.709610749030364 42-43 22.759554359058512 26.40333875896449 27.953901354042394 22.883205527934606 44-45 22.68732226205957 26.52604043776533 27.878955473042378 22.907681827132723 46-47 22.87616687304634 26.78754217773725 27.383818296001678 22.952472653214734 48-49 22.988398693245273 26.92325432665143 27.081609192343613 23.006737787759686 50-51 22.836784087707507 26.876706797908472 27.015069736074416 23.27143937830961 52-53 23.17249763166018 26.922258136622162 26.90642485355322 22.99881937816444 54-55 22.852285339566556 26.727345241653566 27.007724759958624 23.412644658821254 56-57 22.779107937650505 27.1369023899192 26.98078693278143 23.103202739648868 58-59 22.97068051124162 26.748068296828116 27.046948968974778 23.234302222955492 60-61 23.017115896868205 26.74017383776266 27.218498225520747 23.02421203984839 62-63 22.888065731922257 26.831369021800356 27.146217691310813 23.134347554966574 64-65 22.741232916468086 26.571409806850365 27.129389754362798 23.557967522318755 66-67 22.606055913402137 26.993432745487773 26.959310798797436 23.441200542312657 68-69 22.609595988491662 26.76047904988351 27.257127625555892 23.37279733606894 70-71 22.589134283767866 26.788784080103323 27.192409770220983 23.429671865907835 72-73 22.693485700692055 26.818686364118516 26.945309036101 23.54251889908843 74-75 22.609343589186338 26.94523201520415 26.850893543225713 23.594530852383798 76-77 22.754166880108322 26.7634327982545 26.991177818743793 23.491222502893383 78-79 22.775795266454843 26.899773630027017 26.897822164742703 23.426608938775438 80-81 22.62533515438224 26.9824363691762 26.96205711999741 23.430171356444156 82-83 22.72854277784332 26.97466051548248 26.859126232624398 23.437670474049803 84-85 22.56321167954432 26.7794081416284 26.780206457890703 23.877173720936575 86-87 22.671428780511818 26.67406145539554 27.014233563855004 23.640276200237633 88-89 22.63377807384765 26.72487928672391 26.887227388907643 23.754115250520798 90-91 22.555101130263104 26.87923510488236 27.00641160257688 23.559252162277655 92-93 22.64693333380642 26.770438232027733 26.941237310927946 23.641391123237902 94-95 22.53161777913792 26.63142861578106 26.96385050550747 23.87310309957355 96-97 22.61400050117421 26.601218341202067 26.925318389767178 23.85946276785655 98-99 22.33533178460013 26.458944821279207 26.915625972947726 24.29009742117294 100-101 22.290964344622378 26.53596472826152 27.092773702135474 24.08029722498063 102-103 22.462991483412 26.33686925302172 27.055407162981616 24.144732100584662 104-105 22.425339639444655 26.273736619323522 27.14638557636698 24.154538164864835 106-107 22.15319248961634 26.360164896695636 27.133134789078145 24.35350782460988 108-109 22.319254585660648 26.395700446968306 26.82557148343982 24.459473483931227 110-111 22.08066636389763 26.373437516631597 27.139067672427558 24.40682844704321 112-113 22.201734564219013 26.459555293392377 27.017090624438683 24.321619517949923 114-115 22.037217110157812 26.500254463647178 26.902850325480625 24.559678100714383 116-117 21.917258085833172 26.355252431981253 27.077817190938582 24.649672291246997 118-119 21.95178526166489 26.462489558041547 26.763257877732787 24.822467302560778 120-121 21.82518861769651 26.373324439291736 27.022237617272065 24.77924932573969 122-123 21.899858142754468 26.361475856437895 26.91012211431827 24.828543886489367 124-125 21.810643187234454 26.362000896845473 26.79498831040434 25.03236760551573 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 91.0 1 72.0 2 87.0 3 148.0 4 141.5 5 129.5 6 250.0 7 542.5 8 921.5 9 1155.5 10 1068.0 11 884.0 12 866.5 13 953.5 14 1121.5 15 1356.5 16 1574.5 17 1782.0 18 2085.0 19 2449.5 20 2910.0 21 3441.0 22 4059.0 23 4868.0 24 5956.0 25 7644.0 26 10108.0 27 12956.0 28 16231.5 29 19847.0 30 23888.5 31 28790.5 32 33532.5 33 37589.5 34 42187.0 35 46382.5 36 50550.0 37 55712.0 38 59424.5 39 61274.0 40 63283.5 41 66964.0 42 70655.0 43 74111.5 44 77811.0 45 80586.0 46 81937.5 47 83523.0 48 87832.5 49 90218.0 50 88375.5 51 85925.0 52 84173.0 53 83281.0 54 81025.0 55 75059.0 56 68226.5 57 61943.5 58 56388.0 59 50454.5 60 42952.5 61 35180.5 62 28290.5 63 22175.5 64 16941.0 65 13187.0 66 10004.0 67 7670.5 68 5762.0 69 4105.5 70 2969.5 71 2213.0 72 1725.5 73 1412.0 74 1096.0 75 846.5 76 578.0 77 321.0 78 192.0 79 119.5 80 75.0 81 48.0 82 33.5 83 23.5 84 14.0 85 14.0 86 16.0 87 8.5 88 4.5 89 3.0 90 2.5 91 1.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10617603934101667 2 2.66105361756934E-4 3 0.0 4 0.18081859331383668 5 0.0 6 1.33052680878467E-4 7 0.03228745055984133 8 0.0 9 0.0 10-11 5.543861703269459E-4 12-13 5.32210723513868E-4 14-15 3.769825958223232E-4 16-17 0.0039028786391016993 18-19 0.0013970531492239037 20-21 0.003104562553830897 22-23 0.0015301058301023709 24-25 0.004324212128550178 26-27 0.0039028786391016993 28-29 0.0030158607665785857 30-31 8.2049153208388E-4 32-33 4.435089362615567E-5 34-35 0.0010422460002146583 36-37 0.0010422460002146583 38-39 4.213334894484789E-4 40-41 0.001374877702410826 42-43 7.096142980184908E-4 44-45 9.757196597754248E-4 46-47 8.870178725231134E-5 48-49 0.0 50-51 0.03951664622090471 52-53 8.870178725231134E-5 54-55 0.13992706939052116 56-57 1.9957902131770053E-4 58-59 0.0 60-61 0.0 62-63 5.32210723513868E-4 64-65 0.05588212596895614 66-67 0.047211526265042714 68-69 0.001463579489663137 70-71 0.002572351830317029 72-73 0.0707396753337183 74-75 0.0043685630221763335 76-77 0.0013970531492239037 78-79 0.0013305268087846702 80-81 6.65263404392335E-5 82-83 1.774035745046227E-4 84-85 2.2175446813077836E-5 86-87 2.2175446813077834E-4 88-89 0.0010200705534015805 90-91 2.439299149438562E-4 92-93 0.0022175446813077837 94-95 0.0035924223837186095 96-97 0.0018183866386723827 98-99 0.0 100-101 0.0010200705534015805 102-103 0.0012640004683454366 104-105 8.870178725231134E-5 106-107 4.435089362615567E-5 108-109 0.0 110-111 8.870178725231134E-5 112-113 4.435089362615567E-5 114-115 1.5522812769154488E-4 116-117 0.0 118-119 9.091933193361914E-4 120-121 0.002350597362186251 122-123 6.430879575792573E-4 124-125 0.007850108171829554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2254746.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.59782807747889 #Duplication Level Percentage of deduplicated Percentage of total 1 79.40199708566013 47.321865713196516 2 11.235515819154223 13.392246803034954 3 3.857235377464229 6.896485526414475 4 1.7999547302720882 4.290935702480031 5 0.9891231811952842 2.9474796650162776 6 0.603690433807484 2.1587183211646246 7 0.4043062454267053 1.686704187391424 8 0.28452124234611503 1.3565478468587575 9 0.21501223633390043 1.153283606602385 >10 1.1049377001661007 11.923617925785555 >50 0.0713478003140647 2.9292650941597755 >100 0.03144984883119197 3.2123746155622293 >500 6.055326856533982E-4 0.2262744742507922 >1k 3.0276634282659805E-4 0.5042005180822184 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4063 0.18019768080307053 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3876 0.1719040636949794 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.435089362615567E-5 0.0 0.0 0.0 0.0 3 4.435089362615567E-5 0.0 0.0 0.0 0.0 4 4.435089362615567E-5 0.0 0.0 4.435089362615567E-5 0.0 5 4.435089362615567E-5 0.0 0.0 4.435089362615567E-5 0.0 6 4.435089362615567E-5 0.0 0.0 4.435089362615567E-5 0.0 7 1.774035745046227E-4 0.0 0.0 1.33052680878467E-4 0.0 8 1.774035745046227E-4 0.0 0.0 1.33052680878467E-4 0.0 9 1.774035745046227E-4 0.0 0.0 1.33052680878467E-4 0.0 10-11 1.774035745046227E-4 0.0 0.0 1.33052680878467E-4 0.0 12-13 2.4392991494385618E-4 0.0 0.0 2.66105361756934E-4 4.435089362615567E-5 14-15 2.66105361756934E-4 4.435089362615567E-5 0.0 2.66105361756934E-4 4.435089362615567E-5 16-17 2.66105361756934E-4 4.435089362615567E-5 0.0 3.9915804263540106E-4 4.435089362615567E-5 18-19 3.548071490092454E-4 4.435089362615567E-5 0.0 5.765616171400237E-4 8.870178725231134E-5 20-21 4.4350893626155674E-4 4.435089362615567E-5 0.0 5.987370639531015E-4 8.870178725231134E-5 22-23 5.32210723513868E-4 4.435089362615567E-5 0.0 6.652634043923351E-4 8.870178725231134E-5 24-25 6.209125107661794E-4 4.435089362615567E-5 0.0 8.2049153208388E-4 8.870178725231134E-5 26-27 6.652634043923351E-4 4.435089362615567E-5 0.0 0.0011309477874669697 8.870178725231134E-5 28-29 7.539651916446464E-4 4.435089362615567E-5 0.0 0.0019292638727377719 8.870178725231134E-5 30-31 7.539651916446464E-4 4.435089362615567E-5 0.0 0.004213334894484789 8.870178725231134E-5 32-33 7.539651916446464E-4 4.435089362615567E-5 0.0 0.009513266682810391 8.870178725231134E-5 34-35 8.2049153208388E-4 4.435089362615567E-5 0.0 0.01711944493969609 8.870178725231134E-5 36-37 9.313687661492692E-4 6.65263404392335E-5 0.0 0.029981204091281237 8.870178725231134E-5 38-39 0.0010200705534015805 8.870178725231134E-5 0.0 0.05350935315995682 8.870178725231134E-5 40-41 0.0010200705534015805 8.870178725231134E-5 0.0 0.08273659205959341 8.870178725231134E-5 42-43 0.0011087723406538919 8.870178725231134E-5 0.0 0.1107663568313238 8.870178725231134E-5 44-45 0.0013305268087846702 8.870178725231134E-5 0.0 0.14451738688082827 8.870178725231134E-5 46-47 0.0015522812769154484 8.870178725231134E-5 0.0 0.18782603450676927 8.870178725231134E-5 48-49 0.0015966321705416042 8.870178725231134E-5 0.0 0.23381791119709272 8.870178725231134E-5 50-51 0.0015966321705416042 8.870178725231134E-5 0.0 0.2870611589952926 8.870178725231134E-5 52-53 0.001618807617354682 8.870178725231134E-5 0.0 0.339594792495474 8.870178725231134E-5 54-55 0.00164098306416776 8.870178725231134E-5 0.0 0.3944346724642155 8.870178725231134E-5 56-57 0.0016853339577939156 8.870178725231134E-5 0.0 0.46016269681817823 8.870178725231134E-5 58-59 0.0016853339577939156 8.870178725231134E-5 0.0 0.5350491807059421 8.870178725231134E-5 60-61 0.0016853339577939156 8.870178725231134E-5 0.0 0.6184510361699278 8.870178725231134E-5 62-63 0.0016853339577939156 8.870178725231134E-5 0.0 0.7071971743158654 8.870178725231134E-5 64-65 0.0016853339577939156 8.870178725231134E-5 0.0 0.7924395918653364 1.33052680878467E-4 66-67 0.0017296848514200712 8.870178725231134E-5 0.0 0.8946462262268122 1.33052680878467E-4 68-69 0.0017296848514200712 1.1087723406538917E-4 0.0 1.0161454993156656 1.33052680878467E-4 70-71 0.0017518602982331492 1.33052680878467E-4 0.0 1.1556955861103644 1.5522812769154485E-4 72-73 0.001774035745046227 1.33052680878467E-4 0.0 1.300501253799763 1.774035745046227E-4 74-75 0.0017962111918593047 1.33052680878467E-4 0.0 1.4432889558291708 1.774035745046227E-4 76-77 0.0018183866386723825 1.33052680878467E-4 0.0 1.60423834879849 2.4392991494385618E-4 78-79 0.0018183866386723825 1.33052680878467E-4 0.0 1.7915543480285585 2.66105361756934E-4 80-81 0.002128842894055472 1.33052680878467E-4 0.0 2.004460812880919 2.66105361756934E-4 82-83 0.0022175446813077837 1.33052680878467E-4 0.0 2.2247073506284076 2.66105361756934E-4 84-85 0.0022397201281208617 1.33052680878467E-4 0.0 2.4496329076534566 2.66105361756934E-4 86-87 0.0022618955749339393 1.33052680878467E-4 0.0 2.6854909599573524 2.66105361756934E-4 88-89 0.0022618955749339393 1.33052680878467E-4 0.0 2.9537695155019676 2.66105361756934E-4 90-91 0.0023727728089993285 1.33052680878467E-4 0.0 3.2607885766290305 2.66105361756934E-4 92-93 0.002528000936690873 1.33052680878467E-4 0.0 3.5871668028239103 2.66105361756934E-4 94-95 0.002528000936690873 1.9957902131770053E-4 0.0 3.918268399189975 2.66105361756934E-4 96-97 0.002528000936690873 2.2175446813077837E-4 0.0 4.245511467810565 2.66105361756934E-4 98-99 0.0026167027239431847 2.2175446813077837E-4 0.0 4.613091674184143 2.66105361756934E-4 100-101 0.0027941062984478075 2.2175446813077837E-4 0.0 5.037684954314145 2.66105361756934E-4 102-103 0.0029493344261393526 2.2175446813077837E-4 0.0 5.492104210407735 2.66105361756934E-4 104-105 0.003370667915587831 2.2175446813077837E-4 0.0 5.946235185692757 2.66105361756934E-4 106-107 0.0035924223837186095 2.2175446813077837E-4 0.0 6.402495003871833 2.66105361756934E-4 108-109 0.0035924223837186095 2.2175446813077837E-4 0.0 6.880974619757613 2.66105361756934E-4 110-111 0.0035924223837186095 2.2175446813077837E-4 0.0 7.415425063399603 2.66105361756934E-4 112-113 0.0036145978305316875 2.2175446813077837E-4 0.0 7.989503030496562 2.66105361756934E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3940 0.0 64.174446 1 GTATCAA 6985 0.0 50.285336 1 ATCAACG 8610 0.0 39.439354 3 TCAACGC 8745 0.0 38.626144 4 CAACGCA 8865 0.0 38.112915 5 TATCAAC 9150 0.0 38.023304 2 AACGCAG 9100 0.0 37.259415 6 CGCAGAG 11180 0.0 31.224316 8 ACGCAGA 10980 0.0 30.93402 7 GTACATG 9030 0.0 30.576357 1 TACATGG 9180 0.0 29.527512 2 GCAGAGT 12170 0.0 27.316046 9 ACATGGG 9260 0.0 27.085024 3 GAGTACT 6945 0.0 25.991493 12-13 CAGAGTA 11830 0.0 24.258131 10-11 GTACTTT 7775 0.0 23.484577 14-15 AGTACTT 7440 0.0 22.743334 12-13 AGAGTAC 11400 0.0 22.668861 10-11 CATGGGA 5225 0.0 21.435305 4 CATGGGG 4410 0.0 21.344042 4 >>END_MODULE