FastQCFastQC Report
Thu 26 May 2016
SRR1364481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783604
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70700.034.937621
TACATGG65950.033.200092
GTACATG68650.033.033081
ACATGGG69450.029.7278463
TATCAAC82950.029.2650992
CAACGCA79250.028.9354885
TCAACGC78750.028.8923034
ATCAACG80750.028.5150833
AACGCAG80750.027.3507426
GGTATCA50400.025.6267641
TATACCG1401.0487892E-425.5265775
CGCATAG2851.1641532E-1025.0660748
AACGCAT3800.025.065376
ACGCAGA89300.024.0654227
ACGCATA3553.6379788E-1223.4767197
CGCAGAG90150.023.3108928
CATGGGG38750.022.287724
CATGGGA40050.022.1591514
GAGTACT33500.021.5747812-13
AGAGTAC65750.021.54194310-11
Produced by FastQC (version 0.11.5)