##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364481_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1783604 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.72770020699662 30.0 26.0 31.0 2.0 34.0 2 25.90140132002395 30.0 26.0 31.0 2.0 34.0 3 25.829753129057796 30.0 26.0 31.0 2.0 34.0 4 28.533554533405397 35.0 27.0 37.0 2.0 37.0 5 29.117571501297373 35.0 28.0 37.0 2.0 37.0 6 29.27886907631963 35.0 29.0 37.0 2.0 37.0 7 29.019288474347444 35.0 28.0 37.0 2.0 37.0 8 28.692087481301904 35.0 27.0 37.0 2.0 37.0 9 29.23029046806354 35.0 27.0 39.0 2.0 39.0 10-11 29.361238537253783 35.0 27.0 39.0 2.0 39.0 12-13 29.211288492288645 35.0 27.0 39.0 2.0 39.0 14-15 29.820226911354762 36.0 27.0 40.0 2.0 41.0 16-17 30.06511198674145 36.0 27.0 40.0 2.0 41.0 18-19 29.99165285567873 36.0 27.0 40.0 2.0 41.0 20-21 29.769776531113408 36.0 26.0 40.0 2.0 41.0 22-23 29.800921336798975 36.0 27.0 40.0 2.0 41.0 24-25 29.81407756430239 36.0 26.5 40.0 2.0 41.0 26-27 29.89292914794988 36.0 27.0 40.0 2.0 41.0 28-29 29.8232202888085 36.0 27.0 40.0 2.0 41.0 30-31 29.459389247837525 36.0 25.0 40.0 2.0 41.0 32-33 29.46654246121897 36.0 25.5 40.0 2.0 41.0 34-35 29.15265972715917 35.0 25.0 40.0 2.0 41.0 36-37 28.91355928782398 35.0 24.0 40.0 2.0 41.0 38-39 28.763849486769484 35.0 24.0 40.0 2.0 41.0 40-41 28.817840170800245 35.0 24.0 40.0 2.0 41.0 42-43 28.681242304906245 35.0 24.0 40.0 2.0 41.0 44-45 28.324355350178628 35.0 23.5 39.5 2.0 41.0 46-47 28.066612039443733 34.5 22.5 39.0 2.0 41.0 48-49 27.823252526906195 34.0 21.0 39.0 2.0 41.0 50-51 27.82266915750357 34.0 21.5 39.0 2.0 41.0 52-53 27.653308974413605 34.0 20.5 39.0 2.0 40.0 54-55 27.52217784889471 34.0 20.0 39.0 2.0 40.0 56-57 27.11874328606574 33.0 18.0 39.0 2.0 40.0 58-59 26.847608269548623 33.0 15.5 38.0 2.0 40.0 60-61 26.449589146469734 33.0 13.0 38.0 2.0 40.0 62-63 26.39758068495025 33.0 13.0 38.0 2.0 40.0 64-65 26.201846373970902 32.5 12.5 37.5 2.0 40.0 66-67 25.962778453064693 32.0 12.5 37.0 2.0 40.0 68-69 25.777265020710875 32.0 10.5 37.0 2.0 40.0 70-71 25.64549922516433 32.0 9.0 36.0 2.0 39.0 72-73 25.626076752462993 32.0 9.5 36.0 2.0 39.0 74-75 25.343984987699063 32.0 8.0 36.0 2.0 39.0 76-77 25.061937234946768 32.0 7.0 35.0 2.0 38.5 78-79 24.778618740482756 32.0 7.0 35.0 2.0 37.0 80-81 24.767695351658777 32.0 4.0 35.0 2.0 37.0 82-83 24.56746032191002 32.0 2.0 35.0 2.0 36.5 84-85 24.387708818773675 32.0 2.0 35.0 2.0 36.0 86-87 24.19248050576249 32.0 2.0 35.0 2.0 36.0 88-89 23.96438250867345 32.0 2.0 35.0 2.0 35.5 90-91 23.804385670810337 31.5 2.0 35.0 2.0 35.0 92-93 23.691340959091818 31.0 2.0 35.0 2.0 35.0 94-95 23.640617536179555 31.5 2.0 35.0 2.0 35.0 96-97 23.52586644793351 32.0 2.0 35.0 2.0 35.0 98-99 23.428476276124073 31.5 2.0 35.0 2.0 35.0 100-101 23.35972334666215 31.0 2.0 35.0 2.0 35.0 102-103 22.27840260506256 29.5 2.0 33.5 2.0 35.0 104-105 23.03905491353462 31.0 2.0 34.0 2.0 35.0 106-107 23.11083429954183 31.0 2.0 35.0 2.0 35.0 108-109 23.071930764900728 31.0 2.0 35.0 2.0 35.0 110-111 22.960749415228943 31.0 2.0 35.0 2.0 35.0 112-113 22.936683815465763 31.0 2.0 35.0 2.0 35.0 114-115 22.883622429642454 31.0 2.0 35.0 2.0 35.0 116-117 22.70116068364951 31.0 2.0 35.0 2.0 35.0 118-119 22.55817771209304 31.0 2.0 35.0 2.0 35.0 120-121 22.4016771660077 31.0 2.0 35.0 2.0 35.0 122-123 22.139574984133247 31.0 2.0 35.0 2.0 35.0 124-125 21.16263391425451 29.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.366708780848267 1101 2 1.9768430599643771 1101 3 2.2128107289427916 1101 4 2.69308386320823 1101 5 2.1910206230484377 1101 6 2.1473058024311236 1101 7 1.9774338081509342 1101 8 2.512290132862166 1101 9 2.9006516023532463 1101 10-11 3.0129100829328053 1101 12-13 2.7878207475199908 1101 14-15 3.0361390551319793 1101 16-17 2.7679376170135157 1101 18-19 2.8083474986852153 1101 20-21 3.057770452586162 1101 22-23 2.954605085008211 1101 24-25 2.914772115404112 1101 26-27 2.564308886896569 1101 28-29 2.492286617124808 1101 30-31 2.642272624105498 1101 32-33 2.6844867625493087 1101 34-35 2.9618272811399073 1101 36-37 3.066150195639704 1101 38-39 3.1293657685231615 1101 40-41 2.830342970362757 1101 42-43 2.85832316098778 1101 44-45 2.993906352392745 1101 46-47 2.9657416525842173 1101 48-49 2.9541064579504663 1101 50-51 2.904319455630027 1101 52-53 2.800848509920634 1101 54-55 2.7660386780507586 1101 56-57 3.111988781189414 1101 58-59 3.053726155183586 1101 60-61 3.3550215308567424 1101 62-63 2.9381287772787417 1101 64-65 2.765866911325894 1101 66-67 2.894905025618346 1101 68-69 2.7800744323277904 1101 70-71 2.7208303903773547 1101 72-73 2.5538317575441845 1101 74-75 2.5479056132420403 1101 76-77 2.7063003828035086 1101 78-79 2.64178076817187 1101 80-81 2.6134143638783414 1101 82-83 2.54713901140034 1101 84-85 2.3798787248068116 1101 86-87 2.311360837523381 1101 88-89 2.4078206991556 1101 90-91 2.477699585375664 1101 92-93 2.4819254070776573 1101 94-95 2.37598232321017 1101 96-97 2.402805069922227 1101 98-99 2.3389320296244556 1101 100-101 2.336711275726401 1101 102-103 2.3804271345747274 1101 104-105 2.3908368743542816 1101 106-107 2.5278280017935373 1101 108-109 2.5531244182570134 1101 110-111 2.6516911958816145 1101 112-113 2.5376505042151436 1101 114-115 2.531406838049982 1101 116-117 2.5623071966124193 1101 118-119 2.5653377501184202 1101 120-121 2.5057104515163466 1101 122-123 2.562558447788394 1101 124-125 2.438443156430232 1102 1 1.4384711286861105 1102 2 1.3598220585007965 1102 3 1.3833146347565446 1102 4 1.9879801490115234 1102 5 1.8208917223777767 1102 6 1.8378542621474594 1102 7 1.6741087233633287 1102 8 1.7319555671451603 1102 9 2.2068756297203898 1102 10-11 1.9487130817983882 1102 12-13 2.4807121077071415 1102 14-15 2.8102707536466447 1102 16-17 2.274871979357826 1102 18-19 2.335625890042465 1102 20-21 2.2515008720864316 1102 22-23 2.255958747773956 1102 24-25 2.1134349105443277 1102 26-27 2.1076907514597565 1102 28-29 1.8240600740491821 1102 30-31 1.7015648753706962 1102 32-33 1.9727255314979466 1102 34-35 2.2190352561709155 1102 36-37 2.381708794601682 1102 38-39 2.353173011177354 1102 40-41 1.9189787862928327 1102 42-43 1.9811335247662782 1102 44-45 2.3743743447045524 1102 46-47 2.324412030116825 1102 48-49 2.283278390167407 1102 50-51 2.3627594224790975 1102 52-53 2.4954744158401923 1102 54-55 2.171441867357821 1102 56-57 2.3459919904810818 1102 58-59 2.4199536504666312 1102 60-61 2.7448758196107015 1102 62-63 2.596568450236642 1102 64-65 2.8223260516354216 1102 66-67 2.7939157879132885 1102 68-69 2.5512043263506357 1102 70-71 2.9068602732323328 1102 72-73 2.6323656520137426 1102 74-75 2.8111007393507457 1102 76-77 2.6338664936239944 1102 78-79 2.453601365241184 1102 80-81 2.1089486874290166 1102 82-83 2.1308101236608934 1102 84-85 2.18559461432724 1102 86-87 2.18111502694212 1102 88-89 1.911337107100067 1102 90-91 1.7899351567882853 1102 92-93 1.8579047994282583 1102 94-95 1.9921986506284526 1102 96-97 2.0824916055918017 1102 98-99 2.3049223547268767 1102 100-101 2.2295679567545506 1102 102-103 1.8559451177759065 1102 104-105 1.8233923757032393 1102 106-107 2.1840402735135633 1102 108-109 2.266868533086175 1102 110-111 2.0645404120738426 1102 112-113 1.9416597676650724 1102 114-115 2.077809903922752 1102 116-117 2.2130413367749995 1102 118-119 2.2772388733705036 1102 120-121 2.231365681897639 1102 122-123 2.348977148671832 1102 124-125 2.15710142564631 1103 1 1.633000436304748 1103 2 1.4365812875804949 1103 3 1.586161368176679 1103 4 2.212309060179173 1103 5 1.6695514376452785 1103 6 1.5968179764117103 1103 7 1.7026320173729665 1103 8 1.8642580411483998 1103 9 2.678777949686271 1103 10-11 2.1122121433180965 1103 12-13 2.43009663854685 1103 14-15 2.649345310922339 1103 16-17 2.4282942528995477 1103 18-19 2.2487391778941586 1103 20-21 2.574878584820958 1103 22-23 2.3613987391611175 1103 24-25 2.571444586977144 1103 26-27 2.2451304068514446 1103 28-29 2.198873309667775 1103 30-31 2.424334956867341 1103 32-33 2.4777683620467954 1103 34-35 2.2369346456411243 1103 36-37 2.692862483542047 1103 38-39 2.6462006911700655 1103 40-41 2.796481641958529 1103 42-43 2.751480031921858 1103 44-45 2.5932034596581843 1103 46-47 2.7317798546467422 1103 48-49 2.740181943496925 1103 50-51 2.6902802314236745 1103 52-53 2.649118576528842 1103 54-55 2.617566828094265 1103 56-57 2.799546769277164 1103 58-59 2.8937125228469185 1103 60-61 2.8365860187885765 1103 62-63 2.7491234650577105 1103 64-65 2.538657679160785 1103 66-67 2.625562360907413 1103 68-69 2.6180656495457377 1103 70-71 2.7053795270147916 1103 72-73 2.4424345724922496 1103 74-75 2.6359951048605765 1103 76-77 2.567539687160341 1103 78-79 2.7313998732556826 1103 80-81 2.260859454604862 1103 82-83 2.3947610635904653 1103 84-85 2.3040084672783507 1103 86-87 2.4022626880764975 1103 88-89 2.5194064404744125 1103 90-91 2.4521512243517662 1103 92-93 2.3659643723709216 1103 94-95 2.075419581253076 1103 96-97 2.24314085767654 1103 98-99 2.2069669581250437 1103 100-101 1.9765431398217999 1103 102-103 2.0583507979922224 1103 104-105 2.3513524040735625 1103 106-107 2.175183598650108 1103 108-109 2.4524371728783336 1103 110-111 2.5577916946832957 1103 112-113 2.465413641521252 1103 114-115 2.4342291032242613 1103 116-117 2.5333338657856643 1103 118-119 2.6630496901766634 1103 120-121 2.5831540000214623 1103 122-123 2.5914628289354695 1103 124-125 2.611684658171143 1104 1 1.4522532131475607 1104 2 1.41705924113149 1104 3 1.387238446601451 1104 4 1.754550729650731 1104 5 1.2494908519838859 1104 6 1.2107526785322094 1104 7 1.335415591926992 1104 8 1.696301124285391 1104 9 2.184331635314006 1104 10-11 2.1039657616284266 1104 12-13 1.603102360525984 1104 14-15 1.9234954287277937 1104 16-17 1.674844236070193 1104 18-19 0.9486718604926097 1104 20-21 -3.587692939918192 1104 22-23 -0.32187288655413226 1104 24-25 1.1072911033016162 1104 26-27 1.4140970847376835 1104 28-29 0.9538379680319657 1104 30-31 1.7740488579107598 1104 32-33 1.5708346696873186 1104 34-35 1.9006842249073657 1104 36-37 2.0928961422428216 1104 38-39 2.099919977605609 1104 40-41 2.0973904268794286 1104 42-43 2.12391355598783 1104 44-45 2.237430992475417 1104 46-47 2.1728771282919794 1104 48-49 2.254991771837549 1104 50-51 2.181360675718526 1104 52-53 2.18748949541137 1104 54-55 1.9622319240215624 1104 56-57 2.348015298391939 1104 58-59 2.4048198941023884 1104 60-61 2.497474608489018 1104 62-63 2.0868885273263054 1104 64-65 2.2264732294805434 1104 66-67 2.2554849458956348 1104 68-69 2.092989917468998 1104 70-71 2.288179930919206 1104 72-73 2.2229798434447936 1104 74-75 2.1674659900844055 1104 76-77 2.1415888288634726 1104 78-79 2.148767762855144 1104 80-81 1.9404286232349541 1104 82-83 1.9664540962394028 1104 84-85 1.908013852670475 1104 86-87 1.8357867540475503 1104 88-89 1.904798564338435 1104 90-91 1.8637971348196238 1104 92-93 1.5970313531854643 1104 94-95 1.8228110319430826 1104 96-97 1.895614772183439 1104 98-99 1.7581281833017535 1104 100-101 1.2885592959981729 1104 102-103 1.7749445374738784 1104 104-105 1.9605748880856773 1104 106-107 1.9532044670445856 1104 108-109 1.9610201415757373 1104 110-111 2.076135686605209 1104 112-113 1.9780356543115616 1104 114-115 1.9581267807739096 1104 116-117 1.9665213447489762 1104 118-119 2.010575775669764 1104 120-121 1.9974926065512513 1104 122-123 1.8464274872996569 1104 124-125 2.0372989294602704 1105 1 1.487209736658123 1105 2 1.1823447947507368 1105 3 1.2865953834468087 1105 4 1.7412823805373314 1105 5 1.4926730767107053 1105 6 1.3900004243091075 1105 7 1.3243553726086823 1105 8 1.543699716433835 1105 9 1.971069031412366 1105 10-11 1.7075591398026404 1105 12-13 1.9142614844658823 1105 14-15 2.057890580959313 1105 16-17 1.901700903680279 1105 18-19 1.5534535361511423 1105 20-21 1.7329630076177267 1105 22-23 1.727554017128078 1105 24-25 1.5275465340431467 1105 26-27 1.6049111892554464 1105 28-29 1.144547468494956 1105 30-31 1.5935128456759777 1105 32-33 1.5715130510162503 1105 34-35 1.4861271973288588 1105 36-37 1.7207010673542165 1105 38-39 1.4210372587791333 1105 40-41 1.6226263267621341 1105 42-43 1.9358815302089951 1105 44-45 1.968280868375409 1105 46-47 2.119074307393003 1105 48-49 1.8490863808261047 1105 50-51 1.9467125510552172 1105 52-53 1.9782730885057163 1105 54-55 1.93035114883914 1105 56-57 2.185764338157618 1105 58-59 2.0524175315156654 1105 60-61 2.159450325012486 1105 62-63 2.0757439037515475 1105 64-65 1.6643548440132285 1105 66-67 2.0351789069341706 1105 68-69 1.4535103100523585 1105 70-71 1.6615179252749357 1105 72-73 1.5091800851328045 1105 74-75 1.8479553653910514 1105 76-77 1.745427007992994 1105 78-79 1.595898767760204 1105 80-81 1.510476030387693 1105 82-83 1.5818742560501384 1105 84-85 1.5458692360769533 1105 86-87 1.5021910331545172 1105 88-89 1.608997181115754 1105 90-91 1.7008190962921397 1105 92-93 1.6006732108756516 1105 94-95 1.5186634125036207 1105 96-97 1.6279834669136726 1105 98-99 1.4099901526436085 1105 100-101 1.5706199523874709 1105 102-103 1.5712760938734895 1105 104-105 1.5424482185265695 1105 106-107 1.6747057275005588 1105 108-109 1.7922760048104003 1105 110-111 1.6132636026006004 1105 112-113 1.8279175055195012 1105 114-115 1.7224439457613983 1105 116-117 1.9346848603424789 1105 118-119 1.994753210698228 1105 120-121 1.9041544885933526 1105 122-123 1.9073278764290293 1105 124-125 1.6112240684354546 1106 1 0.07933684909852445 1106 2 0.3158225212826693 1106 3 0.268242453639882 1106 4 -0.03474709310848212 1106 5 -0.44028167452072964 1106 6 -0.011623241205565904 1106 7 0.09098495798394168 1106 8 0.5128566328289672 1106 9 0.7547422726358199 1106 10-11 -2.091937959246568 1106 12-13 -0.027840419565464458 1106 14-15 0.3105337924952316 1106 16-17 0.13860304187684136 1106 18-19 -2.6230071037338547 1106 20-21 -5.120783452279586 1106 22-23 -6.091042138000336 1106 24-25 -5.612526356572019 1106 26-27 -4.824630093496687 1106 28-29 -1.3367383226197695 1106 30-31 -3.2257844312828965 1106 32-33 -3.7442760704863858 1106 34-35 -3.053701259950852 1106 36-37 -5.590461242231104 1106 38-39 -6.024826424424653 1106 40-41 -2.2129325946519387 1106 42-43 -0.7276505770849084 1106 44-45 -0.04814954273661343 1106 46-47 0.08599207515847596 1106 48-49 0.4072990341565337 1106 50-51 0.4247716421241883 1106 52-53 0.2918022172161727 1106 54-55 0.2967711556348114 1106 56-57 0.37822535986977357 1106 58-59 0.47463836807480675 1106 60-61 0.6826836904529365 1106 62-63 0.4408577158397655 1106 64-65 0.44092792394313207 1106 66-67 0.09425185569981309 1106 68-69 0.18313327586639616 1106 70-71 0.5087616578438556 1106 72-73 0.47546435616328964 1106 74-75 0.43441043775286303 1106 76-77 0.4432120551724523 1106 78-79 0.447052083563122 1106 80-81 -0.04835644069606104 1106 82-83 -0.10819969453806522 1106 84-85 -0.18674676198359208 1106 86-87 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2216 44-45 -4.757192270925291 2216 46-47 -4.864459794098526 2216 48-49 -4.533832019143723 2216 50-51 -4.4969502807003785 2216 52-53 -4.99016416799801 2216 54-55 -5.732156857455191 2216 56-57 -4.621533813506083 2216 58-59 -5.590786561624377 2216 60-61 -5.209108903925866 2216 62-63 -5.407418395467619 2216 64-65 -5.241174000239791 2216 66-67 -5.898044340485722 2216 68-69 -5.410535741118579 2216 70-71 -7.597583703112573 2216 72-73 -8.018292377168422 2216 74-75 -5.807009996931402 2216 76-77 -5.328666950166859 2216 78-79 -5.57303411866021 2216 80-81 -5.465550402396843 2216 82-83 -5.806112135834628 2216 84-85 -5.625594947122877 2216 86-87 -6.1396483403563735 2216 88-89 -6.25232163575923 2216 90-91 -6.200291003408655 2216 92-93 -5.902225250151865 2216 94-95 -8.94315705611627 2216 96-97 -10.938258164113705 2216 98-99 -8.051201749639757 2216 100-101 -6.878037499259985 2216 102-103 -7.683788856714065 2216 104-105 -10.204858077811856 2216 106-107 -7.178878461611614 2216 108-109 -9.311352131482428 2216 110-111 -7.064189258345655 2216 112-113 -6.465697866322309 2216 114-115 -6.8668995320207955 2216 116-117 -6.2514849010775535 2216 118-119 -6.539552581996382 2216 120-121 -7.357016078778434 2216 122-123 -10.13092175754722 2216 124-125 -8.844682767191141 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 199488.0 3 28130.0 4 15834.0 5 13267.0 6 13487.0 7 15137.0 8 16462.0 9 17021.0 10 17837.0 11 18327.0 12 18433.0 13 18475.0 14 17788.0 15 15475.0 16 13248.0 17 12098.0 18 10925.0 19 10358.0 20 10605.0 21 11427.0 22 14244.0 23 19379.0 24 25234.0 25 31872.0 26 37930.0 27 43651.0 28 49430.0 29 55774.0 30 62722.0 31 69964.0 32 82369.0 33 98811.0 34 120788.0 35 148728.0 36 188213.0 37 187819.0 38 52708.0 39 146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43831517560823 18.389508736920433 13.854604265596993 23.317571821874346 2 15.049150245863393 19.871022666092387 40.862787588385196 24.21703949965902 3 19.471162340171706 25.194665631665647 28.44100914842935 26.893162879733296 4 11.633392210787644 16.30279128494014 37.95469932392827 34.10911718034394 5 13.610030380133098 38.501041091710306 33.69008674268431 14.198841785472283 6 31.99894591654799 36.53369142267625 17.398432331206475 14.068930329569284 7 28.658982048853893 32.54692993743423 20.46386923254183 18.330218781170053 8 25.931688423677773 35.630925288313335 19.532026623113396 18.905359664895492 9 25.93879293765528 16.974107464758646 20.170990873496695 36.91610872408938 10-11 24.760405743138353 26.9679739795771 27.083834080258434 21.187786197026117 12-13 25.72964553465848 24.1043048427729 27.591935645748844 22.574113976819778 14-15 22.65322102904602 25.591101633735246 25.527600349551438 26.228076987667293 16-17 22.527963967459907 28.90316535232608 25.79672142914421 22.772149251069802 18-19 22.50274629915511 27.740425243185562 27.6697700734982 22.08705838416113 20-21 23.232637108185635 26.80906117254274 27.87118136520536 22.087120354066265 22-23 22.85289722403685 26.775609879187705 27.568165441908327 22.803327454867116 24-25 22.71001729875825 26.858046445904233 27.770675100161775 22.66126115517575 26-27 22.546601734071473 27.007601105809087 27.617052067280422 22.828745092839014 28-29 22.53755469484147 27.36996028106028 27.44411089904751 22.64837412505074 30-31 22.646935805082848 26.93679186888305 27.58173503897805 22.834537287056058 32-33 22.562519396481033 26.99673368165465 27.444447216760913 22.9962997051034 34-35 22.895047111948532 26.846899135147716 27.579072509361406 22.67898124354235 36-37 22.882520765426996 26.66485754422281 27.66465934439309 22.7879623459571 38-39 22.642681100769487 26.940194671990398 27.655713341723388 22.761410885516735 40-41 22.87066060290973 26.89263863721381 27.48824628320078 22.74845447667568 42-43 22.604068392506935 27.00065745850932 27.250211325949426 23.14506282303432 44-45 22.733633135722698 26.862829181115146 27.444463403490538 22.959074279671622 46-47 22.947825996759818 27.14784040629276 27.075596502800746 22.82873709414667 48-49 22.970394848430562 26.92272001027219 27.07172841053314 23.035156730764108 50-51 22.862260565024908 27.215047743312304 27.04018938643136 22.882502305231426 52-53 23.04798122031505 27.34924791825129 26.651710354816167 22.951060506617495 54-55 22.866469072338365 27.031369501393925 26.842241899417534 23.259919526850176 56-57 22.84265382462119 27.131456311169533 26.888144853159474 23.137745011049798 58-59 23.155171625862376 26.914654021696556 26.86687931644041 23.063295036000653 60-61 22.910248058889625 26.880631631665597 27.288777270816624 22.92034303862816 62-63 22.75425246973215 27.122197844342534 27.072744328124642 23.050805357800673 64-65 22.71097175941423 27.112465005548085 26.98241152073707 23.194151714300613 66-67 22.742763425335372 27.1403860440993 26.722269726236703 23.394580804328626 68-69 22.70771825524207 27.04819427417024 26.977882280115917 23.266205190471773 70-71 22.731070080329054 26.87252703753604 27.122059007400455 23.274343874734445 72-73 22.72308684686281 26.970859671780595 27.042232436931396 23.2638210444252 74-75 22.510605767103996 27.369901515574142 26.95276330237783 23.166729414944033 76-77 22.78336223294724 26.93823425928911 26.963269688767276 23.315133818996365 78-79 22.73252232506483 26.96321639071506 27.03620096637971 23.268060317840398 80-81 22.916012541265967 27.10083586012631 26.783099119061287 23.200052479546432 82-83 22.796843566585853 27.115269027973703 26.851578777392277 23.236308628048167 84-85 22.77452881469398 27.11259304071827 26.832507223612545 23.28037092097521 86-87 22.726578249396358 27.155496858653567 26.865877921595153 23.252046970354918 88-89 22.71529819814238 27.00459568589203 26.828399225015914 23.45170689094967 90-91 22.759839498838964 27.09476134100834 26.838557171600165 23.30684198855253 92-93 22.814139633563098 27.017937219730943 26.91651051776397 23.25141262894199 94-95 22.66134693319159 26.91379766622485 26.9732297644919 23.451625636091656 96-97 22.583898863855534 26.859634801096227 27.077035124940007 23.47943121010823 98-99 22.573937840015386 26.935189696556154 26.815989827081694 23.674882636346766 100-101 22.462343676849457 26.822655748458846 27.038487523583388 23.676513051108312 102-103 22.448335398917525 26.538864893973997 27.326823823002467 23.685975884106007 104-105 22.64393939181561 26.64505065586726 27.077305459407043 23.633704492910084 106-107 22.31873161539512 26.766498943283818 27.221872272883115 23.692897168437952 108-109 22.29428691972477 26.66285411524278 27.095746902652383 23.947112062380068 110-111 22.235058568125392 26.926311587361035 27.135527516814644 23.70310232769892 112-113 22.327384843798885 26.851772940689667 27.11142596664824 23.709416248863214 114-115 22.29768617194997 26.94664798118594 26.940929080302617 23.814736766561477 116-117 22.075038168923346 26.86865124509467 27.248434134988187 23.807876450993792 118-119 22.20877835010796 26.721499349330298 27.21649611700729 23.853226183554458 120-121 22.1194151697905 27.012540566484176 27.164125210542018 23.703919053183306 122-123 22.109589594102026 26.83323549349127 27.16572275048261 23.8914521619241 124-125 22.128922328258994 26.939956385462754 26.971874934262658 23.959246352015594 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 282.0 1 294.0 2 377.0 3 464.5 4 441.5 5 365.5 6 348.0 7 424.0 8 576.0 9 685.0 10 662.0 11 645.0 12 700.5 13 789.0 14 916.5 15 1023.0 16 1095.0 17 1222.5 18 1378.5 19 1617.5 20 1951.0 21 2352.0 22 2953.5 23 3749.5 24 4829.0 25 6312.5 26 8363.5 27 11075.0 28 13887.5 29 16979.5 30 20372.5 31 23576.5 32 27163.5 33 30475.5 34 33685.5 35 36912.0 36 40680.0 37 44326.0 38 46686.0 39 48965.5 40 51064.5 41 52914.5 42 55056.5 43 57453.5 44 59954.0 45 62299.0 46 64343.0 47 66587.0 48 68983.0 49 69600.0 50 67422.0 51 65616.5 52 65127.5 53 63970.0 54 62322.0 55 58718.5 56 53536.5 57 48934.0 58 45288.0 59 41123.0 60 35369.0 61 29627.0 62 23945.0 63 18542.0 64 13987.5 65 10314.0 66 7749.5 67 5968.5 68 4549.0 69 3558.0 70 2736.0 71 2036.0 72 1485.5 73 1121.5 74 851.5 75 605.5 76 420.5 77 275.5 78 189.0 79 117.0 80 81.0 81 56.5 82 49.5 83 39.5 84 26.0 85 21.0 86 16.0 87 16.0 88 16.5 89 14.5 90 12.0 91 7.5 92 5.0 93 5.0 94 3.5 95 3.0 96 3.0 97 2.0 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09116373365388282 2 0.02803312842985326 3 0.037844723380301905 4 0.04513333677206375 5 0.04821698089934761 6 0.003588240439021218 7 0.0022987165312479676 8 0.0022987165312479676 9 0.0065597520525856635 10-11 0.0476843514591804 12-13 0.005662691942830359 14-15 0.009839628078878495 16-17 0.018333665993124033 18-19 0.016539545773613426 20-21 0.13206406803303872 22-23 0.014521160526663992 24-25 0.01339983538946986 26-27 0.006363520153576691 28-29 0.00403677049389887 30-31 0.002018385246949435 32-33 0.005466460043821386 34-35 0.019707289286186844 36-37 0.0028313459714151795 38-39 0.00798944160250818 40-41 0.030892507529698295 42-43 0.012418675894424995 44-45 0.11134758612337717 46-47 0.0035041410537316577 48-49 0.008269772886806713 50-51 0.008998634225982897 52-53 0.010512423161194974 54-55 0.008522071042675392 56-57 0.004905797475224321 58-59 0.013091470976741475 60-61 0.030247745575811672 62-63 0.006223354511427425 64-65 0.005999089483988598 66-67 0.007232547134902141 68-69 0.0069802489790334625 70-71 0.003644306695880924 72-73 5.886956970269186E-4 74-75 0.0012614907793433968 76-77 0.007372712777051408 78-79 0.019651223029327137 80-81 0.0029154453567047395 82-83 0.00403677049389887 84-85 0.002438882173397234 86-87 0.002999544741994299 88-89 0.004681532447785495 90-91 0.0036723398243107778 92-93 0.002803312842985326 94-95 0.003139710384143566 96-97 0.001569855192071783 98-99 0.0025229815586867935 100-101 0.0021865840175285547 102-103 4.765631833075055E-4 104-105 0.002775279714555473 106-107 7.008282107463315E-4 108-109 0.0012054245224836903 110-111 0.0022426502743882614 112-113 0.0039246379801794575 114-115 0.00249494843025694 116-117 0.0047656318330750545 118-119 0.003195776641003272 120-121 0.006279420768287132 122-123 0.004317101778197403 124-125 0.0527863808334137 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1783604.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.92687415038431 #Duplication Level Percentage of deduplicated Percentage of total 1 85.8432885491797 62.60282700681154 2 8.54492620000259 12.46309515223821 3 2.5067980118884403 5.484388294002655 4 1.0697877368321946 3.1206510260634355 5 0.5682583127304006 2.0720651228699825 6 0.3499576235955771 1.5312789344353326 7 0.21769039981048868 1.1112836273508446 8 0.165455275479623 0.9652908841935706 9 0.12095888781525721 0.7939038230166345 >10 0.5806759453294911 7.26458378888953 >50 0.023030644226701057 1.120792117185205 >100 0.008619072728518407 1.0845806981034312 >500 3.161945033429962E-4 0.15097930503593276 >1k 2.3714587750724714E-4 0.23428021980375363 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 5.606625685970653E-5 0.0 0.0 0.0 3 0.0 5.606625685970653E-5 0.0 0.0 0.0 4 0.0 5.606625685970653E-5 0.0 0.0 0.0 5 5.606625685970653E-5 5.606625685970653E-5 0.0 0.0 0.0 6 5.606625685970653E-5 5.606625685970653E-5 0.0 0.0 0.0 7 5.606625685970653E-5 5.606625685970653E-5 0.0 0.0 0.0 8 5.606625685970653E-5 5.606625685970653E-5 0.0 0.0 0.0 9 5.606625685970653E-5 5.606625685970653E-5 0.0 0.0 0.0 10-11 5.606625685970653E-5 5.606625685970653E-5 0.0 0.0 0.0 12-13 5.606625685970653E-5 5.606625685970653E-5 0.0 0.0 2.8033128429853264E-5 14-15 5.606625685970653E-5 5.606625685970653E-5 0.0 2.8033128429853264E-5 5.606625685970653E-5 16-17 5.606625685970653E-5 5.606625685970653E-5 0.0 1.1213251371941305E-4 5.606625685970653E-5 18-19 8.409938528955979E-5 5.606625685970653E-5 0.0 1.1213251371941305E-4 5.606625685970653E-5 20-21 1.1213251371941305E-4 5.606625685970653E-5 0.0 2.522981558686794E-4 5.606625685970653E-5 22-23 1.6819877057911957E-4 5.606625685970653E-5 0.0 5.045963117373588E-4 5.606625685970653E-5 24-25 1.6819877057911957E-4 5.606625685970653E-5 0.0 8.409938528955978E-4 5.606625685970653E-5 26-27 1.6819877057911957E-4 5.606625685970653E-5 0.0 0.001065258880334424 5.606625685970653E-5 28-29 2.242650274388261E-4 5.606625685970653E-5 0.0 0.0016819877057911957 5.606625685970653E-5 30-31 2.242650274388261E-4 5.606625685970653E-5 0.0 0.0029715116135644462 1.401656421492663E-4 32-33 2.242650274388261E-4 5.606625685970653E-5 0.0 0.00669991769473493 1.6819877057911957E-4 34-35 2.803312842985326E-4 5.606625685970653E-5 0.0 0.012166377738556316 1.6819877057911957E-4 36-37 3.3639754115823915E-4 8.409938528955979E-5 0.0 0.021417310120407894 1.6819877057911957E-4 38-39 3.924637980179457E-4 1.1213251371941305E-4 0.0 0.037928822765591466 1.6819877057911957E-4 40-41 3.924637980179457E-4 1.6819877057911957E-4 0.0 0.05766414518020816 1.6819877057911957E-4 42-43 3.924637980179457E-4 1.6819877057911957E-4 0.0 0.07854882586044884 1.6819877057911957E-4 44-45 3.924637980179457E-4 1.6819877057911957E-4 0.0 0.10363847580516752 1.6819877057911957E-4 46-47 4.485300548776522E-4 1.6819877057911957E-4 0.0 0.13363392322511053 1.6819877057911957E-4 48-49 4.485300548776522E-4 1.6819877057911957E-4 0.0 0.16601218656159103 1.6819877057911957E-4 50-51 4.485300548776522E-4 1.6819877057911957E-4 0.0 0.20352051240073468 1.6819877057911957E-4 52-53 4.485300548776522E-4 1.6819877057911957E-4 0.0 0.23912258550664833 1.6819877057911957E-4 54-55 4.485300548776522E-4 1.6819877057911957E-4 0.0 0.2797145554730759 1.6819877057911957E-4 56-57 4.485300548776522E-4 1.6819877057911957E-4 0.0 0.3301461535183819 1.6819877057911957E-4 58-59 5.045963117373588E-4 1.6819877057911957E-4 0.0 0.3887914581936349 1.6819877057911957E-4 60-61 5.045963117373588E-4 1.6819877057911957E-4 0.0 0.4581454179290919 1.6819877057911957E-4 62-63 5.045963117373588E-4 1.6819877057911957E-4 0.0 0.5285366034164534 1.6819877057911957E-4 64-65 5.045963117373588E-4 1.6819877057911957E-4 0.0 0.6013666710772122 1.6819877057911957E-4 66-67 5.045963117373588E-4 1.6819877057911957E-4 0.0 0.6807284576621268 1.6819877057911957E-4 68-69 5.045963117373588E-4 1.6819877057911957E-4 0.0 0.7788724402950431 1.6819877057911957E-4 70-71 5.045963117373588E-4 1.6819877057911957E-4 0.0 0.8916777490967727 1.6819877057911957E-4 72-73 5.045963117373588E-4 1.6819877057911957E-4 0.0 1.0108185449236489 1.6819877057911957E-4 74-75 5.045963117373588E-4 1.6819877057911957E-4 0.0 1.13480907196889 1.6819877057911957E-4 76-77 5.045963117373588E-4 1.6819877057911957E-4 0.0 1.269115790276317 1.6819877057911957E-4 78-79 5.045963117373588E-4 1.6819877057911957E-4 0.0 1.4298857818215254 1.6819877057911957E-4 80-81 5.045963117373588E-4 1.6819877057911957E-4 0.0 1.6107274933225089 1.6819877057911957E-4 82-83 5.32629440167212E-4 1.6819877057911957E-4 0.0 1.8050251064698217 1.6819877057911957E-4 84-85 5.606625685970652E-4 1.6819877057911957E-4 0.0 2.003836053294341 1.6819877057911957E-4 86-87 5.606625685970652E-4 1.6819877057911957E-4 0.0 2.205926876145153 1.6819877057911957E-4 88-89 5.606625685970652E-4 1.6819877057911957E-4 0.0 2.437929047030619 1.6819877057911957E-4 90-91 5.606625685970652E-4 1.6819877057911957E-4 0.0 2.7058696885631566 1.6819877057911957E-4 92-93 5.606625685970652E-4 1.6819877057911957E-4 0.0 2.9994326094805794 1.6819877057911957E-4 94-95 5.606625685970652E-4 1.6819877057911957E-4 0.0 3.2864077452169878 1.6819877057911957E-4 96-97 5.606625685970652E-4 1.6819877057911957E-4 0.0 3.572934350898518 1.6819877057911957E-4 98-99 5.886956970269186E-4 1.9623189900897284E-4 0.0 3.876617231179118 1.6819877057911957E-4 100-101 6.447619538866251E-4 2.242650274388261E-4 0.0 4.242645789087712 1.6819877057911957E-4 102-103 6.727950823164783E-4 2.242650274388261E-4 0.0 4.635193686490947 1.6819877057911957E-4 104-105 6.727950823164783E-4 2.242650274388261E-4 0.0 5.031469989975353 1.6819877057911957E-4 106-107 7.288613391761849E-4 2.242650274388261E-4 0.0 5.413645629859543 1.6819877057911957E-4 108-109 7.568944676060381E-4 2.242650274388261E-4 0.0 5.816005122213227 1.6819877057911957E-4 110-111 8.129607244657447E-4 2.242650274388261E-4 0.0 6.2729451156198355 1.6819877057911957E-4 112-113 8.40993852895598E-4 2.242650274388261E-4 0.0 6.779587845732573 1.6819877057911957E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7070 0.0 34.93762 1 TACATGG 6595 0.0 33.20009 2 GTACATG 6865 0.0 33.03308 1 ACATGGG 6945 0.0 29.727846 3 TATCAAC 8295 0.0 29.265099 2 CAACGCA 7925 0.0 28.935488 5 TCAACGC 7875 0.0 28.892303 4 ATCAACG 8075 0.0 28.515083 3 AACGCAG 8075 0.0 27.350742 6 GGTATCA 5040 0.0 25.626764 1 TATACCG 140 1.0487892E-4 25.526577 5 CGCATAG 285 1.1641532E-10 25.066074 8 AACGCAT 380 0.0 25.06537 6 ACGCAGA 8930 0.0 24.065422 7 ACGCATA 355 3.6379788E-12 23.476719 7 CGCAGAG 9015 0.0 23.310892 8 CATGGGG 3875 0.0 22.28772 4 CATGGGA 4005 0.0 22.159151 4 GAGTACT 3350 0.0 21.57478 12-13 AGAGTAC 6575 0.0 21.541943 10-11 >>END_MODULE