FastQCFastQC Report
Thu 26 May 2016
SRR1364481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783604
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33320.18681276785654216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33020.18513078015075096No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18130.10164812368664794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18060.10125565988862999No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67750.033.0234571
GTATCAA91950.031.5993211
GTACATG67350.027.372941
TATCAAC109950.026.1225322
GAGTACT59150.025.94104612-13
CAACGCA107200.025.6391535
ATCAACG108850.025.5646423
TACATGG67600.025.5631712
TCAACGC107800.025.5372734
GTACTTT61150.025.14158614-15
AACGCAG109850.024.5873816
ACATGGG69200.022.8187073
AGAGTAC91650.022.29034610-11
AGTACTT61050.021.96763812-13
CGCAGAG127500.021.3625668
ACGCAGA126500.021.2100897
ACTTTTT79250.020.41259616-17
GAGTACA55650.020.2626951
GCAGAGT130950.019.936889
GTATAGG8300.019.4082431
Produced by FastQC (version 0.11.5)