##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364481_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1783604 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.79739224626094 31.0 28.0 31.0 24.0 34.0 2 28.947233242356486 31.0 28.0 33.0 25.0 34.0 3 29.049354565251033 31.0 28.0 34.0 25.0 34.0 4 32.47080854270342 35.0 32.0 37.0 26.0 37.0 5 31.62428263224348 35.0 32.0 37.0 19.0 37.0 6 31.673282858751158 35.0 32.0 37.0 19.0 37.0 7 31.15774353499992 35.0 31.0 37.0 17.0 37.0 8 31.237128869412718 35.0 31.0 37.0 17.0 37.0 9 31.771179028528753 37.0 30.0 39.0 11.0 39.0 10-11 31.952684284179675 37.0 30.5 39.0 14.0 39.0 12-13 31.721593750630745 37.0 30.0 39.0 11.5 39.0 14-15 32.65739816685767 37.0 31.0 40.0 12.0 41.0 16-17 32.70976825573389 37.0 31.0 40.0 11.0 41.0 18-19 32.54736169015095 37.0 31.0 40.0 10.0 41.0 20-21 32.43321864046055 37.0 30.5 40.0 10.0 41.0 22-23 32.41665526652777 37.0 30.5 40.0 10.0 41.0 24-25 32.23407185675744 37.0 30.0 40.0 10.0 41.0 26-27 32.29813316184534 37.0 30.0 40.0 10.0 41.0 28-29 32.16511568711441 37.0 30.0 40.0 9.0 41.0 30-31 31.682557058629605 37.0 30.0 40.0 8.0 41.0 32-33 31.603686412454785 37.0 30.0 40.0 7.5 41.0 34-35 31.402619359454228 36.0 30.0 40.0 2.0 41.0 36-37 31.37545245469286 36.0 30.0 40.0 2.0 41.0 38-39 31.14693984763434 36.0 30.0 40.0 2.0 41.0 40-41 30.864755573546596 36.0 28.5 40.0 2.0 41.0 42-43 30.740694963680276 36.0 28.5 40.0 2.0 41.0 44-45 30.65933273305061 36.0 28.0 40.0 2.0 41.0 46-47 30.513149779883875 36.0 28.0 40.0 2.0 41.0 48-49 30.23873012170863 36.0 28.0 40.0 2.0 41.0 50-51 29.894774008131847 35.0 27.0 40.0 2.0 41.0 52-53 29.789654822483016 35.0 27.0 40.0 2.0 41.0 54-55 29.319574860787483 35.0 26.0 39.0 2.0 41.0 56-57 29.316701465123423 35.0 26.0 39.0 2.0 41.0 58-59 29.063207976658497 35.0 26.0 39.0 2.0 41.0 60-61 28.76757817318194 34.0 25.5 39.0 2.0 41.0 62-63 28.508186234164086 34.0 25.0 39.0 2.0 40.0 64-65 28.04061103249376 34.0 23.0 38.0 2.0 40.0 66-67 27.640845165182405 34.0 22.5 38.0 2.0 40.0 68-69 27.451969439404714 33.5 22.0 37.0 2.0 40.0 70-71 27.507790126059373 33.5 23.5 37.0 2.0 39.5 72-73 27.28584764331096 34.0 23.0 36.5 2.0 39.0 74-75 26.937301385285075 33.0 22.0 36.0 2.0 39.0 76-77 26.853237882399903 33.0 23.0 36.0 2.0 39.0 78-79 26.466229611505693 33.0 22.0 35.0 2.0 37.5 80-81 26.229585154552243 33.0 21.0 35.0 2.0 37.0 82-83 26.07564823806181 33.0 21.0 35.0 2.0 36.5 84-85 25.67060008836042 33.0 19.5 35.0 2.0 36.0 86-87 25.54145426899693 32.0 20.0 35.0 2.0 36.0 88-89 25.495362479563852 32.0 20.0 35.0 2.0 35.5 90-91 25.33824324233406 32.0 19.0 35.0 2.0 35.0 92-93 25.34426195500795 32.0 19.5 35.0 2.0 35.0 94-95 25.350862074765473 32.5 20.0 35.0 2.0 35.0 96-97 25.234732877925815 32.0 19.0 35.0 2.0 35.0 98-99 25.10735987360423 32.0 18.5 35.0 2.0 35.0 100-101 25.01790363780301 32.0 18.0 35.0 2.0 35.0 102-103 23.878438823864492 30.0 16.0 34.0 2.0 35.0 104-105 24.817196249840208 32.0 18.0 34.5 2.0 35.0 106-107 24.929318951964675 32.0 17.5 35.0 2.0 35.0 108-109 25.003816990767007 33.0 18.0 35.0 2.0 35.0 110-111 24.93774458904555 33.0 17.0 35.0 2.0 35.0 112-113 24.871015651456265 33.0 15.5 35.0 2.0 35.0 114-115 24.81752087346743 33.0 11.5 35.0 2.0 35.0 116-117 24.731793324078666 33.0 7.0 35.0 2.0 35.0 118-119 24.61133328922788 33.0 2.0 35.0 2.0 35.0 120-121 24.443868425951052 33.0 2.0 35.0 2.0 35.0 122-123 24.146898638935546 32.0 2.0 35.0 2.0 35.0 124-125 23.372723149308925 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1012361634854848 1101 2 1.0520616646041674 1101 3 1.0149997652461344 1101 4 1.117788093243206 1101 5 1.4230424971216245 1101 6 1.440127361280286 1101 7 1.89634837579149 1101 8 1.7366316320801154 1101 9 2.5441375614343826 1101 10-11 2.1886867792363596 1101 12-13 2.338128593400242 1101 14-15 2.3462416210276587 1101 16-17 2.2718927083249483 1101 18-19 2.2595835214077624 1101 20-21 2.5291888442618315 1101 22-23 2.56911684910618 1101 24-25 2.730508850659035 1101 26-27 2.3538624497053036 1101 28-29 2.513571231321194 1101 30-31 2.9079170204245557 1101 32-33 2.7376889458894205 1101 34-35 2.834597666607227 1101 36-37 2.6086456702277268 1101 38-39 2.7672866357076664 1101 40-41 2.9427916222300965 1101 42-43 2.7478967513518846 1101 44-45 2.602981260658286 1101 46-47 2.432821252415703 1101 48-49 2.5136894655066904 1101 50-51 2.6594123167136914 1101 52-53 2.5399919462879303 1101 54-55 2.830656124439617 1101 56-57 2.62839205476363 1101 58-59 2.499255755172559 1101 60-61 2.8050283571231063 1101 62-63 2.889958027603871 1101 64-65 2.970168114234717 1101 66-67 3.166575933119315 1101 68-69 3.0805122152428446 1101 70-71 2.7020182120693157 1101 72-73 2.9195749258965478 1101 74-75 3.062119846671589 1101 76-77 2.851132164667362 1101 78-79 2.895540921310616 1101 80-81 2.939075423119842 1101 82-83 2.850812151359772 1101 84-85 3.1353669581430275 1101 86-87 3.0388194377617843 1101 88-89 2.7960370637570264 1101 90-91 2.7493509747099694 1101 92-93 2.6637938803594494 1101 94-95 2.593824614583937 1101 96-97 2.55011984679982 1101 98-99 2.5233398622982364 1101 100-101 2.629091366921532 1101 102-103 2.445042222947688 1101 104-105 2.4813759492566305 1101 106-107 2.5899575437201285 1101 108-109 2.713946750420604 1101 110-111 2.722520976242695 1101 112-113 2.5552616038852847 1101 114-115 2.604559553328972 1101 116-117 2.6362326570680708 1101 118-119 2.5665744810094075 1101 120-121 2.3821869363453345 1101 122-123 2.498603936585738 1101 124-125 2.2879912677776346 1102 1 0.7280842981581799 1102 2 -1.375911485497813 1102 3 0.1867098004542953 1102 4 0.8051942684846409 1102 5 1.1403068404805659 1102 6 0.6721838354090472 1102 7 1.4481990085391985 1102 8 1.1743272901318527 1102 9 2.081978401728044 1102 10-11 1.8013185159568899 1102 12-13 1.9393720469742561 1102 14-15 1.8022140775464592 1102 16-17 1.8440521031442358 1102 18-19 1.9423359298459602 1102 20-21 2.0646518110344942 1102 22-23 1.9868049835437631 1102 24-25 2.146278169595618 1102 26-27 1.798770785046898 1102 28-29 1.8146656820679752 1102 30-31 2.103354065995326 1102 32-33 1.9012505542970537 1102 34-35 2.316069591182991 1102 36-37 1.7267237982584724 1102 38-39 1.3886210779903756 1102 40-41 1.6297837048017598 1102 42-43 1.6588680029168579 1102 44-45 1.6838751012869757 1102 46-47 1.2271112407188376 1102 48-49 1.3331559354621945 1102 50-51 1.5293713482834796 1102 52-53 1.3471196890273518 1102 54-55 1.6734757751793374 1102 56-57 1.2709304516460342 1102 58-59 1.2814662282785783 1102 60-61 1.35184992403331 1102 62-63 1.5704859737159822 1102 64-65 1.6548328725680008 1102 66-67 1.8028344633107558 1102 68-69 1.6115571159370141 1102 70-71 1.0199394022110333 1102 72-73 1.3325123094421691 1102 74-75 1.483464869036709 1102 76-77 1.4993191499904341 1102 78-79 1.4340823981725634 1102 80-81 1.4373135455078838 1102 82-83 1.1026272232749434 1102 84-85 1.2643663762384953 1102 86-87 1.3026249758471415 1102 88-89 1.4094120512666528 1102 90-91 1.1490006564258266 1102 92-93 1.158954374331742 1102 94-95 1.1838602341947073 1102 96-97 1.207839780262855 1102 98-99 0.9730012996612096 1102 100-101 0.8405381344124656 1102 102-103 0.6101993665605505 1102 104-105 0.923014745243254 1102 106-107 1.2452566955501894 1102 108-109 1.103126852812288 1102 110-111 1.0326728592034726 1102 112-113 1.0713727182618555 1102 114-115 1.310037390995241 1102 116-117 0.9475074982198919 1102 118-119 0.5439495625642117 1102 120-121 0.8876500794527047 1102 122-123 1.230724478991295 1102 124-125 1.3350344286360318 1103 1 0.6686900009713703 1103 2 0.5229048994867398 1103 3 0.4962977978444165 1103 4 0.6331184494513238 1103 5 0.922973378585592 1103 6 0.9094708139875571 1103 7 1.1777473844921005 1103 8 1.096537477774671 1103 9 1.7553656087006573 1103 10-11 1.5586233117597779 1103 12-13 1.4503437186522632 1103 14-15 1.331804571240923 1103 16-17 1.2953901400197338 1103 18-19 1.5130293168281526 1103 20-21 1.7570488488322127 1103 22-23 1.5945828645126383 1103 24-25 1.8351024753183722 1103 26-27 1.4517628897449697 1103 28-29 1.469477545310717 1103 30-31 2.1259356824292865 1103 32-33 1.9367635004744876 1103 34-35 2.035957860859572 1103 36-37 2.011182174158133 1103 38-39 1.8862358651035258 1103 40-41 2.0982550259915413 1103 42-43 2.0494393667567685 1103 44-45 2.0123539705439235 1103 46-47 2.032954367511582 1103 48-49 2.0562950204306 1103 50-51 2.2845115761097006 1103 52-53 2.207469029665827 1103 54-55 2.3025293268229667 1103 56-57 2.2275271724959147 1103 58-59 2.4092776614707887 1103 60-61 2.2906942534295425 1103 62-63 2.321650452369923 1103 64-65 2.5008533671083697 1103 66-67 2.7148795458573893 1103 68-69 2.835025689417389 1103 70-71 2.543851868293533 1103 72-73 2.504731044856978 1103 74-75 2.6086600924589085 1103 76-77 2.4927232868754636 1103 78-79 2.4799113516354154 1103 80-81 2.4241768326201516 1103 82-83 2.270671629098313 1103 84-85 2.4588597181452307 1103 86-87 2.4105043653055205 1103 88-89 2.3073475129261958 1103 90-91 2.395361405530082 1103 92-93 2.223711939997262 1103 94-95 2.123137571832885 1103 96-97 2.0903699680949686 1103 98-99 2.1656545340020763 1103 100-101 2.1094399202165413 1103 102-103 1.9928005985044912 1103 104-105 2.0619915207397206 1103 106-107 2.069882323841007 1103 108-109 2.0165062751012073 1103 110-111 2.0874422497798655 1103 112-113 2.08052161453449 1103 114-115 1.8756632210016484 1103 116-117 1.9004638146684663 1103 118-119 2.00939881748835 1103 120-121 2.0036735139534088 1103 122-123 2.091234482250684 1103 124-125 1.7378047940725097 1104 1 0.6979730706448812 1104 2 0.5865198439498833 1104 3 0.615786185592647 1104 4 0.592054834506861 1104 5 1.0034176734358091 1104 6 1.004388350170661 1104 7 1.3810459371679684 1104 8 1.33484107925565 1104 9 1.8082097689160719 1104 10-11 1.2503096126685698 1104 12-13 1.425099693071651 1104 14-15 1.2922555978313 1104 16-17 1.1634480329850732 1104 18-19 1.2807842814865111 1104 20-21 1.4160862099900697 1104 22-23 1.4348723293392993 1104 24-25 1.6062233100541476 1104 26-27 1.6232539701892676 1104 28-29 1.4696458388145928 1104 30-31 1.9500689708843524 1104 32-33 1.7913579131301596 1104 34-35 1.8297983186179074 1104 36-37 1.6777927429901816 1104 38-39 1.866040644639039 1104 40-41 2.05163772541934 1104 42-43 1.8962922782343803 1104 44-45 1.6848548120114408 1104 46-47 1.6640550070268922 1104 48-49 1.4354602846513949 1104 50-51 1.8999609197650358 1104 52-53 1.6860957546742377 1104 54-55 2.002293715917549 1104 56-57 1.875962042909915 1104 58-59 1.911633770524979 1104 60-61 1.996180621655732 1104 62-63 2.025453885557397 1104 64-65 2.140368681817222 1104 66-67 2.1278718043562073 1104 68-69 2.1988762447859536 1104 70-71 2.031734736014837 1104 72-73 2.1617488839683894 1104 74-75 2.142487086736928 1104 76-77 2.0814139634153506 1104 78-79 2.0522775582998385 1104 80-81 1.9002791550705034 1104 82-83 1.8585208381188423 1104 84-85 2.2222390517029567 1104 86-87 2.1986228439322453 1104 88-89 1.9397945004724768 1104 90-91 1.9872496586435133 1104 92-93 1.710080166183733 1104 94-95 1.751882102294008 1104 96-97 1.7876099546314883 1104 98-99 1.904799603002413 1104 100-101 1.965380680903177 1104 102-103 1.9031001609413813 1104 104-105 1.796760958639414 1104 106-107 1.9364255752714996 1104 108-109 1.873793383818814 1104 110-111 1.9711514386051725 1104 112-113 1.848781459704469 1104 114-115 1.8080092324456025 1104 116-117 1.9129175339549036 1104 118-119 1.8991665724530087 1104 120-121 1.9550366913347617 1104 122-123 1.941116676797975 1104 124-125 1.722658378724141 1105 1 0.548029452688187 1105 2 0.630810162089265 1105 3 0.43078899353628586 1105 4 0.7362543851672143 1105 5 0.811407317405493 1105 6 0.9291044457247501 1105 7 1.2506279135002103 1105 8 1.1497516433610606 1105 9 1.7087677068016376 1105 10-11 1.4073197266446407 1105 12-13 1.5154969378420446 1105 14-15 1.4116377220512462 1105 16-17 1.4520539962630963 1105 18-19 1.604970615870883 1105 20-21 1.8310219408697037 1105 22-23 1.7182751564641592 1105 24-25 1.920520040628091 1105 26-27 1.644387479869323 1105 28-29 1.7548404967747828 1105 30-31 1.9947732418312825 1105 32-33 1.7147031118898788 1105 34-35 1.962442039610739 1105 36-37 1.7495458600250622 1105 38-39 1.8650158339077159 1105 40-41 1.919987113491345 1105 42-43 1.9473056958216652 1105 44-45 1.8443254102035773 1105 46-47 1.698874309244605 1105 48-49 1.8676054997998506 1105 50-51 1.8375082934421911 1105 52-53 1.7957614897849545 1105 54-55 1.9376342187105564 1105 56-57 1.9417385946864592 1105 58-59 1.8974479042265848 1105 60-61 1.6539564968248648 1105 62-63 1.5487627933480113 1105 64-65 1.8689069683318458 1105 66-67 2.209409406137727 1105 68-69 2.120863994209113 1105 70-71 1.9730198735067965 1105 72-73 2.1649970458725782 1105 74-75 2.1723183549031546 1105 76-77 1.809724730388531 1105 78-79 2.050815047122711 1105 80-81 2.060061448167634 1105 82-83 1.8222991663331811 1105 84-85 2.1752880825074783 1105 86-87 2.0662809182265285 1105 88-89 1.8653755250680497 1105 90-91 1.9452394571159033 1105 92-93 1.8588995766677847 1105 94-95 1.7282731731362446 1105 96-97 1.8136906409805782 1105 98-99 1.9161444506404273 1105 100-101 1.9230419036192892 1105 102-103 1.7613438130832293 1105 104-105 1.3454560917240421 1105 106-107 1.4978741056703662 1105 108-109 1.540917759985522 1105 110-111 1.671336704517497 1105 112-113 1.7173856055728791 1105 114-115 1.5498341795055381 1105 116-117 1.562681013200212 1105 118-119 1.5806571252991723 1105 120-121 1.6619055154467048 1105 122-123 1.831592397498742 1105 124-125 1.5929252026097096 1106 1 0.727713950233138 1106 2 0.6483540778381105 1106 3 0.6028095899375181 1106 4 0.5242397625134672 1106 5 0.8067353826136134 1106 6 0.9582836677424389 1106 7 1.1717583398071554 1106 8 0.9452578550991397 1106 9 1.743123150225074 1106 10-11 1.3655037903112408 1106 12-13 1.2240760043101773 1106 14-15 1.3265674837373354 1106 16-17 1.4167165085460525 1106 18-19 1.5660879032494677 1106 20-21 1.2665749880197907 1106 22-23 1.1985873120099342 1106 24-25 1.1451722066267394 1106 26-27 1.0726951079682436 1106 28-29 0.95932131665149 1106 30-31 1.3962262457652628 1106 32-33 1.3512769071535367 1106 34-35 1.3826314585690547 1106 36-37 1.3641110028294854 1106 38-39 1.4093310563877672 1106 40-41 1.7238954176517325 1106 42-43 1.812849181896521 1106 44-45 1.675453940402324 1106 46-47 1.516433467287289 1106 48-49 1.632997818323286 1106 50-51 2.0221910671985874 1106 52-53 1.741276210915526 1106 54-55 2.1962870036387123 1106 56-57 1.927858987963699 1106 58-59 1.818030342238 1106 60-61 1.9701953074474758 1106 62-63 2.0408393468510084 1106 64-65 2.0481024570865998 1106 66-67 2.1596866038911564 1106 68-69 1.9555417168090763 1106 70-71 1.8532482497311413 1106 72-73 1.7472044095230324 1106 74-75 2.093820837667934 1106 76-77 1.8262902732587563 1106 78-79 1.761204476479417 1106 80-81 1.8909557184034362 1106 82-83 1.7281040997911532 1106 84-85 2.040324735664523 1106 86-87 2.0937028352238904 1106 88-89 1.8774280698856636 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0.22969655378400233 2204 20-21 0.8661121756060624 2204 22-23 0.9205515530756188 2204 24-25 1.27533797216595 2204 26-27 1.250082445357009 2204 28-29 1.2520379738516212 2204 30-31 1.482912972815427 2204 32-33 1.1487530276372446 2204 34-35 1.194722356097781 2204 36-37 1.2223299428376713 2204 38-39 1.3337953299720873 2204 40-41 1.5096532680110997 2204 42-43 1.3398481384097174 2204 44-45 1.333128663018389 2204 46-47 1.1017092469757195 2204 48-49 1.2852088685884233 2204 50-51 1.6073931379851487 2204 52-53 1.5019340122801523 2204 54-55 1.3380023037278868 2204 56-57 1.188421220678599 2204 58-59 1.1678816959525697 2204 60-61 1.3444112470697718 2204 62-63 1.1284053416104491 2204 64-65 1.4834398776508024 2204 66-67 1.800374857149162 2204 68-69 1.987099063057368 2204 70-71 1.7364344543152548 2204 72-73 1.8364396941188836 2204 74-75 1.9940657752393847 2204 76-77 1.606471539346444 2204 78-79 1.746620592135912 2204 80-81 1.6178927811181474 2204 82-83 1.5891546901127356 2204 84-85 1.913484542344861 2204 86-87 1.6595069993432645 2204 88-89 1.606496548346044 2204 90-91 1.6842458756476262 2204 92-93 1.4316402516200348 2204 94-95 1.3660674988870767 2204 96-97 1.4110546363472736 2204 98-99 1.5999223618240883 2204 100-101 1.637741801519109 2204 102-103 1.577298776605108 2204 104-105 1.4369497383276233 2204 106-107 1.5748694446161515 2204 108-109 1.4280696682148175 2204 110-111 1.4814885121045123 2204 112-113 1.452949493901336 2204 114-115 1.4197757881735669 2204 116-117 1.4465263053285256 2204 118-119 1.3844631786969543 2204 120-121 1.3772784585701814 2204 122-123 0.6959200247912349 2204 124-125 1.1187732196041438 2205 1 0.7730316201844509 2205 2 0.7793247462766644 2205 3 0.6615761461169605 2205 4 0.7264862020143141 2205 5 1.1474694958266518 2205 6 1.0805231775782893 2205 7 1.4180209441404585 2205 8 1.12356698029107 2205 9 0.9808409951723576 2205 10-11 1.3410158673357806 2205 12-13 1.6794558694667856 2205 14-15 1.7229483419266387 2205 16-17 1.4131681618888265 2205 18-19 1.6013087662193684 2205 20-21 1.856794036338485 2205 22-23 1.7840226620000479 2205 24-25 1.9732960473423944 2205 26-27 1.6186394082274163 2205 28-29 1.6170614710265951 2205 30-31 2.011037015838909 2205 32-33 2.030025532459316 2205 34-35 2.1030498115090275 2205 36-37 1.7563622046475764 2205 38-39 1.9081466903844948 2205 40-41 2.122354078874082 2205 42-43 1.889907897284285 2205 44-45 1.9165481695922217 2205 46-47 1.8505677690193814 2205 48-49 1.7506075005564803 2205 50-51 1.7650023621139468 2205 52-53 1.6614136492712923 2205 54-55 1.8714050473387296 2205 56-57 1.801032229954103 2205 58-59 1.960383789508768 2205 60-61 1.9736215808922069 2205 62-63 1.6511944446685618 2205 64-65 2.0507765548687047 2205 66-67 2.1888935337023447 2205 68-69 2.1600560134462548 2205 70-71 1.9008823868105864 2205 72-73 2.098637289540779 2205 74-75 2.139014789707783 2205 76-77 1.83088044534178 2205 78-79 2.0488528237579686 2205 80-81 1.8665611777806497 2205 82-83 1.5926105091830394 2205 84-85 2.1225856338967617 2205 86-87 1.757310361804965 2205 88-89 1.6598013193320398 2205 90-91 1.7709523017403797 2205 92-93 1.4790894148506588 2205 94-95 1.665889419617855 2205 96-97 1.560196749990002 2205 98-99 1.566988037806901 2205 100-101 1.652123661816141 2205 102-103 1.2407309533665583 2205 104-105 1.4606502821442682 2205 106-107 1.5433587025321174 2205 108-109 1.3948430808492986 2205 110-111 1.5186904978262419 2205 112-113 1.5625188599130126 2205 114-115 1.48933373940568 2205 116-117 1.4771695096523558 2205 118-119 1.3019010505118125 2205 120-121 1.23882980911349 2205 122-123 -2.1361063779639906 2205 124-125 -0.04265665388265205 2206 1 0.553942337832698 2206 2 0.7901899157804237 2206 3 0.5358728827417991 2206 4 0.6869082310623185 2206 5 0.9297948441211723 2206 6 0.9834824532854505 2206 7 1.2190611323013911 2206 8 1.130035771064744 2206 9 1.5599010865017462 2206 10-11 1.1199035854595785 2206 12-13 1.0960732753726603 2206 14-15 1.2456774936982242 2206 16-17 1.1637501551655731 2206 18-19 1.470578112889953 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-5.336855219678618 2216 122-123 -5.438564531952803 2216 124-125 -5.296733006936531 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 92147.0 3 30059.0 4 14620.0 5 11935.0 6 12083.0 7 14276.0 8 16105.0 9 17185.0 10 18179.0 11 19417.0 12 20858.0 13 21548.0 14 21774.0 15 17915.0 16 13629.0 17 11811.0 18 10583.0 19 9785.0 20 9697.0 21 10596.0 22 12805.0 23 17596.0 24 24007.0 25 31531.0 26 38393.0 27 44332.0 28 49678.0 29 56255.0 30 62907.0 31 70816.0 32 82452.0 33 97450.0 34 116890.0 35 147260.0 36 202647.0 37 249440.0 38 84573.0 39 370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.67196563016509 20.153216279089346 14.078948476115377 24.095869614630193 2 16.234095351082477 20.84090697567501 38.283738824555755 24.641258848686757 3 20.09566024745403 24.740749628280717 28.940168333329595 26.223421790935657 4 13.500260320280999 17.325704760294727 35.96626563676812 33.20776928265615 5 13.337657910612446 38.63391201185913 33.88891256130845 14.139517516219968 6 31.957055465818346 37.1952727148362 16.750700688437952 14.096971130907496 7 28.094542880651417 32.45723208391547 20.43527056760839 19.01295446782472 8 25.83903153390551 36.48511665145403 18.814658410723457 18.861193403917014 9 25.405807567150557 16.709594730668915 19.596614495145783 38.28798320703475 10-11 25.185439976317507 27.04477359222604 26.54048338300826 21.22930304844819 12-13 26.130293482858928 23.99068399158666 27.30452207099532 22.574500454559086 14-15 22.783512437492956 25.509994118633166 25.46329079574501 26.243202648128864 16-17 22.433774207845808 28.789566359234747 25.983991935133442 22.792667497786002 18-19 22.392376018490875 27.37095144183126 28.09413077331719 22.142541766360683 20-21 23.05380802986846 26.424354525950584 28.30408507510428 22.217752369076678 22-23 22.66942441142352 26.238980748302716 28.35988008407795 22.731714756195814 24-25 22.582340780910698 26.442740336175447 28.343349968853953 22.6315689140599 26-27 22.434922535003974 26.572855502867178 28.386684402854424 22.605537559274424 28-29 22.381485959638464 26.896374627184088 28.32635616124991 22.395783251927543 30-31 22.42807704114028 26.484188512790897 28.242998330616437 22.84473611545238 32-33 22.39794326485679 26.479371676496488 28.251065889924526 22.871619168722194 34-35 22.707274722231098 26.472159754472308 28.195708984198696 22.624856539097895 36-37 22.75939769739489 26.316131775911316 28.249200978813217 22.67526954788058 38-39 22.52310642270246 26.57293275659553 28.280635458161747 22.623325362540264 40-41 22.631342286093616 26.50584441214992 28.12443933114448 22.73837397061198 42-43 22.55014039085172 26.613233295507044 27.811181032433424 23.02544528120781 44-45 22.730974425305963 26.521974490063382 27.83671823992146 22.910332844709195 46-47 22.922730002136127 26.86772224536514 27.442990396405477 22.766557356093255 48-49 22.98607846418781 26.734865680629134 27.257655639271842 23.021400215911218 50-51 22.806997371694848 26.952944027083998 27.234339822020104 23.005718779201054 52-53 23.031533091033456 27.15218217063499 26.82876369594683 22.987521042384724 54-55 22.841794172023175 26.82115152593777 26.924426513628426 23.41262778841063 56-57 22.848583916167232 26.976250540690632 26.986370531260256 23.188795011881876 58-59 23.084608466901845 26.794849080849787 27.04431589074705 23.07622656150132 60-61 22.89888899105407 26.832525605459505 27.32103090147813 22.947554502008295 62-63 22.692763772044664 26.96035254640207 27.282847283155647 23.06403639839762 64-65 22.61461333321366 26.945759281427723 27.1073449243428 23.332282461015815 66-67 22.57861222456693 26.942181072036387 26.931270887814136 23.547935815582548 68-69 22.61395550158138 26.84821837562774 27.107081363266573 23.430744759524302 70-71 22.733698837971104 26.728122355186517 27.150171160344065 23.388007646498313 72-73 22.71450753001275 26.779241654709256 27.036427672744257 23.469823142533734 74-75 22.496579758901035 27.197336697504902 26.89021586767592 23.41586767591814 76-77 22.6790890025379 26.812868521775112 27.000218383274138 23.50782409241285 78-79 22.67005237513074 26.947639727053886 26.922409508316303 23.45989838949907 80-81 22.76026265949466 26.96005050454081 26.906030605482613 23.373656230481913 82-83 22.73515132163771 26.91744764735845 26.957703276208278 23.389697754795563 84-85 22.539784615867646 26.953348388992175 26.871323455206426 23.63554353993375 86-87 22.526609813469896 26.98100105993526 26.97357226215863 23.518816864436214 88-89 22.58661541377033 26.88447465594363 26.781647411321313 23.747262518964728 90-91 22.607788593321747 26.949972681586154 26.804928583863326 23.63731014122877 92-93 22.640937118475627 26.884041821028536 26.995757063501074 23.479263996994764 94-95 22.460852520196106 26.801053202894064 27.04307568438003 23.695018592529795 96-97 22.40502816005113 26.732228610836223 27.109985394338835 23.752757834773806 98-99 22.276665672425043 26.739595784714542 26.860362501990352 24.12337604087006 100-101 22.222617806800603 26.71457413949608 27.080299869307577 23.98250818439574 102-103 22.270916312323948 26.43940515277105 27.170769641351345 24.11890889355365 104-105 22.372097091330506 26.474953170527684 27.06575300838361 24.087196729758205 106-107 21.958187455900372 26.591924718694486 27.200468041243468 24.24941978416167 108-109 22.087133691110807 26.54748475558476 26.972466982581334 24.392914570723097 110-111 21.882674960003655 26.76712077620521 27.069262427320467 24.280941836470667 112-113 22.03177728651195 26.619210238164253 27.01330062048631 24.335711854837488 114-115 21.967073316367635 26.701405416800526 26.922559386947402 24.408961879884437 116-117 21.782021121280284 26.585469644607212 27.123313246662377 24.50919598745013 118-119 21.724358041708683 26.478377403269988 27.233934117540205 24.563330437481127 120-121 21.65065935544641 26.768457467088297 27.110918612213773 24.46996456525152 122-123 21.690649710569126 26.49052905480054 27.040516397718868 24.77830483691147 124-125 21.56413025438136 26.542700048893614 27.03629005127101 24.856879645454015 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 129.0 1 109.5 2 130.5 3 208.5 4 197.5 5 169.0 6 296.5 7 628.5 8 1019.0 9 1255.5 10 1118.0 11 857.5 12 811.0 13 859.0 14 981.0 15 1114.5 16 1288.0 17 1456.5 18 1643.5 19 1916.0 20 2165.5 21 2499.5 22 3066.0 23 3817.5 24 4769.5 25 6125.0 26 8036.0 27 10698.5 28 13605.0 29 16583.0 30 20038.0 31 23436.0 32 26773.5 33 29801.0 34 32988.0 35 36169.0 36 39805.5 37 43811.0 38 46301.5 39 48337.5 40 50531.0 41 52638.5 42 54782.0 43 57345.0 44 59983.0 45 61938.0 46 64311.5 47 66924.0 48 70133.5 49 70913.0 50 67930.0 51 65746.5 52 65320.5 53 65020.5 54 63768.0 55 59700.5 56 54004.0 57 49240.0 58 45480.0 59 41281.0 60 35703.0 61 29870.0 62 24039.0 63 18656.0 64 13828.0 65 9885.5 66 7341.0 67 5698.0 68 4308.0 69 3296.5 70 2529.5 71 1877.0 72 1340.5 73 1047.5 74 781.5 75 527.0 76 377.5 77 217.5 78 133.0 79 75.5 80 40.0 81 22.5 82 21.5 83 17.0 84 9.5 85 6.5 86 5.0 87 3.5 88 2.0 89 1.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10142385865920911 2 3.3639754115823915E-4 3 0.0 4 0.17408572754938875 5 0.0 6 5.6066256859706534E-5 7 0.03739619332542425 8 0.0 9 0.0 10-11 4.4853005487765227E-4 12-13 4.20496926447799E-4 14-15 2.8033128429853267E-4 16-17 0.0036723398243107778 18-19 0.0012054245224836903 20-21 0.0028313459714151795 22-23 0.0011773913940538372 24-25 0.003980704237039163 26-27 0.0031677435125734184 28-29 0.002775279714555473 30-31 6.447619538866251E-4 32-33 2.8033128429853267E-5 34-35 9.53126366615011E-4 36-37 8.690269813254512E-4 38-39 5.045963117373588E-4 40-41 0.0011213251371941307 42-43 6.727950823164783E-4 44-45 0.00137362329306281 46-47 5.6066256859706534E-5 48-49 2.8033128429853267E-5 50-51 0.039582777342952805 52-53 8.409938528955979E-5 54-55 0.1366334679671048 56-57 3.083644127283859E-4 58-59 0.0 60-61 0.0 62-63 5.045963117373588E-4 64-65 0.053711474071598854 66-67 0.04855337844050585 68-69 0.0019623189900897287 70-71 0.0023828159165375272 72-73 0.06932592660702712 74-75 0.005830890713409479 76-77 0.0023828159165375272 78-79 0.001597888320501636 80-81 1.1213251371941307E-4 82-83 1.4016564214926633E-4 84-85 0.0 86-87 2.522981558686794E-4 88-89 0.001681987705791196 90-91 4.765631833075055E-4 92-93 0.0028874122282748858 94-95 0.0051580956310930005 96-97 0.00274724658612562 98-99 0.0 100-101 0.0015137889352120763 102-103 0.0011213251371941307 104-105 2.8033128429853267E-4 106-107 2.8033128429853267E-5 108-109 0.0 110-111 1.9623189900897287E-4 112-113 1.6819877057911957E-4 114-115 2.8033128429853267E-4 116-117 0.0 118-119 0.0010372257519045709 120-121 0.0024669153018270873 122-123 9.811594950448644E-4 124-125 0.007849275960358915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1783604.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.87600511319253 #Duplication Level Percentage of deduplicated Percentage of total 1 82.76429255684562 55.349452522213696 2 10.124945135855976 13.54231765352596 3 3.10999477340874 6.239520791054549 4 1.33327714456427 3.5665699654873135 5 0.718938622750519 2.403987150556765 6 0.43756012542986816 1.755736391134622 7 0.29018061107891296 1.35842840211839 8 0.19915064680950983 1.0654719739462704 9 0.15670575040826115 0.9431869109012592 >10 0.8107321740264215 9.480826764689077 >50 0.039007888015348643 1.7282753002473485 >100 0.014279827038221499 1.696401096138549 >500 5.098602372954664E-4 0.2281117119454167 >1k 4.2488353107955515E-4 0.6417133660407686 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3332 0.18681276785654216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3302 0.18513078015075096 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1813 0.10164812368664794 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1806 0.10125565988862999 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.606625685970653E-5 0.0 5 0.0 0.0 0.0 5.606625685970653E-5 0.0 6 0.0 0.0 0.0 5.606625685970653E-5 0.0 7 0.0 0.0 0.0 5.606625685970653E-5 0.0 8 0.0 0.0 0.0 5.606625685970653E-5 0.0 9 0.0 0.0 0.0 5.606625685970653E-5 0.0 10-11 0.0 0.0 0.0 5.606625685970653E-5 0.0 12-13 0.0 0.0 0.0 5.606625685970653E-5 0.0 14-15 0.0 0.0 0.0 5.606625685970653E-5 0.0 16-17 0.0 0.0 0.0 1.1213251371941305E-4 0.0 18-19 0.0 0.0 0.0 1.1213251371941305E-4 0.0 20-21 0.0 0.0 0.0 3.083644127283859E-4 0.0 22-23 0.0 0.0 0.0 6.727950823164783E-4 0.0 24-25 0.0 0.0 0.0 9.811594950448642E-4 0.0 26-27 0.0 0.0 0.0 0.0011773913940538372 0.0 28-29 0.0 0.0 0.0 0.0017380539626509024 0.0 30-31 0.0 0.0 0.0 0.0030275778704241525 0.0 32-33 0.0 0.0 0.0 0.006727950823164783 0.0 34-35 5.606625685970653E-5 0.0 0.0 0.01211031148169661 0.0 36-37 1.6819877057911957E-4 0.0 0.0 0.020940746937100387 0.0 38-39 1.6819877057911957E-4 0.0 0.0 0.03725602768327499 0.0 40-41 1.6819877057911957E-4 0.0 0.0 0.0566829856851633 0.0 42-43 1.6819877057911957E-4 0.0 0.0 0.07753963323697413 0.0 44-45 2.242650274388261E-4 0.0 0.0 0.10251715066797337 0.0 46-47 3.3639754115823915E-4 0.0 0.0 0.1327929293722149 0.0 48-49 3.924637980179457E-4 0.0 0.0 0.16503102706654615 0.0 50-51 3.924637980179457E-4 0.0 0.0 0.20335231363015557 0.0 52-53 3.924637980179457E-4 0.0 0.0 0.23870208858020053 0.0 54-55 3.924637980179457E-4 0.0 0.0 0.2786212634643116 0.0 56-57 3.924637980179457E-4 0.0 0.0 0.3291930271517669 0.0 58-59 3.924637980179457E-4 0.0 0.0 0.3878102986985901 0.0 60-61 3.924637980179457E-4 0.0 0.0 0.45545423759982595 0.0 62-63 3.924637980179457E-4 0.0 0.0 0.5232103090147813 0.0 64-65 4.204969264477989E-4 0.0 0.0 0.5934613288599936 0.0 66-67 4.485300548776522E-4 0.0 0.0 0.6718419559498633 0.0 68-69 4.485300548776522E-4 0.0 0.0 0.7684160833907079 0.0 70-71 5.045963117373588E-4 0.0 0.0 0.879006775046479 0.0 72-73 5.045963117373588E-4 0.0 0.0 0.9942509660216057 0.0 74-75 5.045963117373588E-4 0.0 0.0 1.1135038943622015 0.0 76-77 5.045963117373588E-4 0.0 0.0 1.2408023305621652 0.0 78-79 5.32629440167212E-4 0.0 0.0 1.391760727156925 0.0 80-81 6.727950823164783E-4 0.0 0.0 1.5631833075054775 0.0 82-83 6.727950823164783E-4 0.0 0.0 1.7486785183258169 0.0 84-85 7.008282107463315E-4 0.0 0.0 1.9372573732734395 0.0 86-87 7.288613391761849E-4 0.0 0.0 2.1306018600541377 0.0 88-89 7.288613391761849E-4 0.0 0.0 2.354081959896928 0.0 90-91 7.288613391761849E-4 0.0 0.0 2.6149021868082825 0.0 92-93 7.288613391761849E-4 0.0 0.0 2.903278978966183 0.0 94-95 7.288613391761849E-4 0.0 0.0 3.184086826448023 0.0 96-97 7.288613391761849E-4 0.0 0.0 3.466296330351356 0.0 98-99 7.568944676060381E-4 0.0 0.0 3.768605587338894 0.0 100-101 7.849275960358914E-4 0.0 0.0 4.1350266090455055 0.0 102-103 7.849275960358914E-4 0.0 0.0 4.5301815873927165 5.606625685970653E-5 104-105 8.970601097553044E-4 0.0 0.0 4.927242818473159 5.606625685970653E-5 106-107 8.970601097553044E-4 0.0 0.0 5.313763593263976 5.606625685970653E-5 108-109 8.970601097553044E-4 0.0 0.0 5.71987952482726 5.606625685970653E-5 110-111 8.970601097553044E-4 0.0 0.0 6.182594342690418 5.606625685970653E-5 112-113 8.970601097553044E-4 0.0 0.0 6.69223661754515 5.606625685970653E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6775 0.0 33.023457 1 GTATCAA 9195 0.0 31.599321 1 GTACATG 6735 0.0 27.37294 1 TATCAAC 10995 0.0 26.122532 2 GAGTACT 5915 0.0 25.941046 12-13 CAACGCA 10720 0.0 25.639153 5 ATCAACG 10885 0.0 25.564642 3 TACATGG 6760 0.0 25.563171 2 TCAACGC 10780 0.0 25.537273 4 GTACTTT 6115 0.0 25.141586 14-15 AACGCAG 10985 0.0 24.587381 6 ACATGGG 6920 0.0 22.818707 3 AGAGTAC 9165 0.0 22.290346 10-11 AGTACTT 6105 0.0 21.967638 12-13 CGCAGAG 12750 0.0 21.362566 8 ACGCAGA 12650 0.0 21.210089 7 ACTTTTT 7925 0.0 20.412596 16-17 GAGTACA 5565 0.0 20.262695 1 GCAGAGT 13095 0.0 19.93688 9 GTATAGG 830 0.0 19.408243 1 >>END_MODULE