FastQCFastQC Report
Thu 26 May 2016
SRR1364480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786748
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52400.053.5021551
ATCAACG60950.042.6623
TCAACGC61150.042.6618964
GGTATCA29250.042.327161
TATCAAC67000.041.0284422
CAACGCA62950.040.874325
AACGCAG63250.040.1893846
ACGCAGA74000.034.1097227
CGCAGAG74600.032.9605378
GTACATG85400.032.0613371
TACATGG89850.029.799732
GCAGAGT81700.028.3472299
ACATGGG93150.026.9564743
AGAGTAC75150.023.36044510-11
TATACCG2653.6907295E-822.4758765
CAGAGTA81900.021.21714810-11
GAGTACA67100.020.2252541
GTATAGG6300.019.8408571
CATGGGA51700.019.5848794
CATGGGG57750.019.3895784
Produced by FastQC (version 0.11.5)