##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364480_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2786748 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.376912444182253 30.0 25.0 31.0 2.0 34.0 2 25.576374325916802 30.0 25.0 31.0 2.0 34.0 3 25.659457546932842 30.0 25.0 31.0 2.0 34.0 4 28.382713829883436 35.0 26.0 37.0 2.0 37.0 5 28.908506617749435 35.0 28.0 37.0 2.0 37.0 6 28.990017037780238 35.0 28.0 37.0 2.0 37.0 7 28.836825934745445 35.0 28.0 37.0 2.0 37.0 8 28.51576353513127 35.0 27.0 37.0 2.0 37.0 9 29.07883310582801 35.0 26.0 39.0 2.0 39.0 10-11 29.172467334685447 35.0 26.5 39.0 2.0 39.0 12-13 28.989379556386154 35.0 26.5 39.0 2.0 39.0 14-15 29.57494093473827 36.0 26.5 40.0 2.0 41.0 16-17 29.786659037702726 36.0 27.0 40.0 2.0 41.0 18-19 29.660287546631416 36.0 26.5 40.0 2.0 41.0 20-21 29.41407457724918 35.5 25.5 40.0 2.0 41.0 22-23 29.45449534726498 36.0 25.5 40.0 2.0 41.0 24-25 29.454742947693873 36.0 25.5 40.0 2.0 41.0 26-27 29.532552100154014 36.0 25.5 40.0 2.0 41.0 28-29 29.46086065191399 36.0 26.0 40.0 2.0 41.0 30-31 29.09554451999248 35.0 25.0 40.0 2.0 41.0 32-33 29.12320812646138 35.0 25.0 40.0 2.0 41.0 34-35 28.82011470000158 35.0 24.0 40.0 2.0 41.0 36-37 28.58378439672335 35.0 24.0 40.0 2.0 41.0 38-39 28.43402758340546 35.0 23.5 39.0 2.0 41.0 40-41 28.48231074356203 35.0 24.0 39.0 2.0 41.0 42-43 28.347106017479874 35.0 23.0 39.0 2.0 41.0 44-45 27.98799855602301 34.0 22.0 39.0 2.0 41.0 46-47 27.743979721166035 34.0 21.0 39.0 2.0 40.0 48-49 27.52623129181397 33.5 19.5 39.0 2.0 40.0 50-51 27.513176290070003 33.5 20.0 39.0 2.0 40.0 52-53 27.336550344702857 33.5 19.0 39.0 2.0 40.0 54-55 27.187255718852224 33.0 19.0 39.0 2.0 40.0 56-57 26.781432336185404 33.0 15.0 38.0 2.0 40.0 58-59 26.503541224394887 33.0 13.5 38.0 2.0 40.0 60-61 26.09046368742348 32.5 12.5 38.0 2.0 40.0 62-63 25.998786937319053 32.0 13.0 37.5 2.0 40.0 64-65 25.78775188858124 32.0 10.5 37.0 2.0 40.0 66-67 25.554270066758818 32.0 9.0 36.5 2.0 40.0 68-69 25.37656526532001 32.0 8.0 36.0 2.0 39.0 70-71 25.264257119768274 32.0 8.0 36.0 2.0 39.0 72-73 25.242131868400012 32.0 7.5 36.0 2.0 39.0 74-75 24.960655574167454 32.0 7.0 35.0 2.0 38.5 76-77 24.688043554709648 32.0 6.5 35.0 2.0 37.0 78-79 24.430698972422334 31.5 2.0 35.0 2.0 37.0 80-81 24.436091099733453 32.0 2.0 35.0 2.0 36.5 82-83 24.264131345927225 32.0 2.0 35.0 2.0 36.0 84-85 24.09463503696782 32.0 2.0 35.0 2.0 36.0 86-87 23.91360790426691 31.0 2.0 35.0 2.0 35.5 88-89 23.713143779057166 31.0 2.0 35.0 2.0 35.0 90-91 23.56576357101539 31.0 2.0 35.0 2.0 35.0 92-93 23.473728697392087 31.0 2.0 35.0 2.0 35.0 94-95 23.429739072208896 31.0 2.0 35.0 2.0 35.0 96-97 23.321985877445684 31.0 2.0 35.0 2.0 35.0 98-99 23.227658905649164 31.0 2.0 34.5 2.0 35.0 100-101 23.16019747748989 31.0 2.0 34.0 2.0 35.0 102-103 22.069416574444478 29.5 2.0 33.5 2.0 34.5 104-105 22.84882755814304 30.5 2.0 34.0 2.0 35.0 106-107 22.920842142884826 31.0 2.0 34.0 2.0 35.0 108-109 22.88097937093702 31.0 2.0 34.0 2.0 35.0 110-111 22.776907886898996 31.0 2.0 34.0 2.0 35.0 112-113 22.75174432707945 31.0 2.0 34.0 2.0 35.0 114-115 22.69949507454567 31.0 2.0 34.0 2.0 35.0 116-117 22.518787848775705 31.0 2.0 34.0 2.0 35.0 118-119 22.362243554135503 31.0 2.0 34.0 2.0 35.0 120-121 22.192423570412537 31.0 2.0 34.0 2.0 35.0 122-123 21.928506990944285 31.0 2.0 34.0 2.0 35.0 124-125 20.921703541188513 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.292944437400113 1101 2 1.9494736579028853 1101 3 2.253317306769631 1101 4 2.5533123479336943 1101 5 2.1116858193993764 1101 6 2.0687957550544276 1101 7 1.9907565774515597 1101 8 2.4810077162324546 1101 9 3.0764364765649397 1101 10-11 3.0004995929017895 1101 12-13 2.817455742379579 1101 14-15 3.0918260650375196 1101 16-17 2.8638348294344063 1101 18-19 2.79625860637827 1101 20-21 3.242451988225767 1101 22-23 3.0556642331141077 1101 24-25 2.9303040090210857 1101 26-27 2.5701394892087492 1101 28-29 2.4697427821720126 1101 30-31 2.684592256302448 1101 32-33 2.907774662817861 1101 34-35 3.1438768421502132 1101 36-37 3.0442836039072425 1101 38-39 3.1935546356646007 1101 40-41 2.8635408010068915 1101 42-43 2.832867715786847 1101 44-45 3.0004893993177184 1101 46-47 2.970746546768808 1101 48-49 3.064609804387601 1101 50-51 2.8920306124303714 1101 52-53 2.937133483340748 1101 54-55 2.9027605704249133 1101 56-57 3.028191657926925 1101 58-59 3.028729939666693 1101 60-61 3.1741318324283974 1101 62-63 2.8726189599273653 1101 64-65 2.7779336759257056 1101 66-67 2.99648556451195 1101 68-69 2.736599295196992 1101 70-71 2.6892745913939997 1101 72-73 2.522985078496763 1101 74-75 2.6488122431398224 1101 76-77 2.800916253350806 1101 78-79 2.7621380100461295 1101 80-81 2.6403254567578927 1101 82-83 2.5407760976002756 1101 84-85 2.385116725414278 1101 86-87 2.464019080769912 1101 88-89 2.659317106693532 1101 90-91 2.6151088257664483 1101 92-93 2.556169122201652 1101 94-95 2.521039561409676 1101 96-97 2.5389371835566976 1101 98-99 2.4915624299643326 1101 100-101 2.450792297767606 1101 102-103 2.499964099006963 1101 104-105 2.4810024359320373 1101 106-107 2.5457487377116337 1101 108-109 2.5856539309674 1101 110-111 2.6751481550242566 1101 112-113 2.504877192072957 1101 114-115 2.516648036692736 1101 116-117 2.5953630429808605 1101 118-119 2.601943119711514 1101 120-121 2.5906855932282333 1101 122-123 2.6418453203378327 1101 124-125 2.658904409404606 1102 1 1.2975126416479341 1102 2 1.062753052392985 1102 3 1.0815711941254484 1102 4 1.7280358452332116 1102 5 1.6943637313350308 1102 6 1.5715711205238065 1102 7 1.6232384124899006 1102 8 1.6954987228859544 1102 9 2.0719916819166535 1102 10-11 1.7768343631885557 1102 12-13 2.0844993997595083 1102 14-15 2.4151327176811286 1102 16-17 2.013050028206756 1102 18-19 2.22447333796379 1102 20-21 2.1405030386323887 1102 22-23 2.2243198433983267 1102 24-25 1.9575765991249945 1102 26-27 1.9984153201884496 1102 28-29 1.773963464987549 1102 30-31 1.8486798020478261 1102 32-33 1.699708359916123 1102 34-35 2.077819455661899 1102 36-37 2.239848886933423 1102 38-39 2.2743337162751907 1102 40-41 1.9131697153265428 1102 42-43 1.8407862810985591 1102 44-45 2.248656510631502 1102 46-47 2.02783131025641 1102 48-49 2.1643061329473596 1102 50-51 2.1263255533632766 1102 52-53 2.2034705858360972 1102 54-55 2.0200319627571623 1102 56-57 2.0931147937713703 1102 58-59 2.1939774890780335 1102 60-61 2.649869644868904 1102 62-63 2.3938407540812676 1102 64-65 2.4781325004329737 1102 66-67 2.4005273825765805 1102 68-69 2.235068829884124 1102 70-71 2.47090833703648 1102 72-73 2.3628570715403754 1102 74-75 2.45996635667516 1102 76-77 2.31761641291709 1102 78-79 2.202985041379314 1102 80-81 1.9123319031165344 1102 82-83 1.905391296223602 1102 84-85 1.9513680279676713 1102 86-87 2.061836602958497 1102 88-89 1.6837822448321837 1102 90-91 1.6972979425486265 1102 92-93 1.7472017336307388 1102 94-95 1.9588298999562035 1102 96-97 2.04374559411184 1102 98-99 2.1583351823169394 1102 100-101 2.101906793561618 1102 102-103 1.7620342098166724 1102 104-105 1.8154359774876383 1102 106-107 2.1157591898061696 1102 108-109 2.2747603746808878 1102 110-111 2.026369826234255 1102 112-113 1.9177526227026043 1102 114-115 1.898381404152314 1102 116-117 2.1343498119815614 1102 118-119 2.2661415478178313 1102 120-121 2.2367520775653666 1102 122-123 2.329514577767835 1102 124-125 2.249133964299247 1103 1 1.5618787068256879 1103 2 1.350901530856369 1103 3 1.3848172221162613 1103 4 2.139514797659878 1103 5 1.5524474465795954 1103 6 1.5530684243572175 1103 7 1.4832825549606632 1103 8 1.837472781288966 1103 9 2.4904689992829 1103 10-11 2.0285340998314965 1103 12-13 2.2722757629812023 1103 14-15 2.436787765320279 1103 16-17 2.3646453656937396 1103 18-19 1.976057621867465 1103 20-21 2.314803722195819 1103 22-23 2.281053045261153 1103 24-25 2.3099162332844827 1103 26-27 1.97287672039473 1103 28-29 1.8271113439666031 1103 30-31 1.9780293256185324 1103 32-33 2.0855637540836867 1103 34-35 1.862680887790244 1103 36-37 2.350364485061821 1103 38-39 2.293709068732266 1103 40-41 2.308876083741243 1103 42-43 2.2995229898501606 1103 44-45 2.053404086123976 1103 46-47 2.2924898840737313 1103 48-49 2.3026641850809355 1103 50-51 2.181202575866447 1103 52-53 2.199053785751076 1103 54-55 2.2490098722323815 1103 56-57 2.3277835020887707 1103 58-59 2.4749252057235402 1103 60-61 2.3232819758644396 1103 62-63 2.274428959784956 1103 64-65 2.320363211882821 1103 66-67 2.344003480171871 1103 68-69 2.146799868308232 1103 70-71 2.278259130681871 1103 72-73 2.2864402177476073 1103 74-75 2.2261953146806235 1103 76-77 2.2892546579749364 1103 78-79 2.410046834408579 1103 80-81 1.9028387283496428 1103 82-83 2.0573396770296632 1103 84-85 2.055326175262298 1103 86-87 1.9907347356309977 1103 88-89 2.1203045605890907 1103 90-91 2.1359050738024123 1103 92-93 2.035756187799308 1103 94-95 1.841979482832361 1103 96-97 1.9649900895408194 1103 98-99 1.9462582395432975 1103 100-101 1.7782509244209166 1103 102-103 1.9035977065539278 1103 104-105 2.0705328707972015 1103 106-107 1.826432024920745 1103 108-109 2.062220769947192 1103 110-111 2.1996446256906736 1103 112-113 2.051094768644699 1103 114-115 2.047245088223697 1103 116-117 2.1485024172421205 1103 118-119 2.297678725869215 1103 120-121 2.259601507903966 1103 122-123 2.2784639956628787 1103 124-125 2.1979268449216818 1104 1 1.6246155492678511 1104 2 1.3815590246792624 1104 3 1.440741643772622 1104 4 1.760501278437232 1104 5 1.4628377885288728 1104 6 1.2802667382950723 1104 7 1.3639075629620976 1104 8 1.6263421466792423 1104 9 2.177030431091481 1104 10-11 2.0045582033508467 1104 12-13 1.6144282767116636 1104 14-15 1.8712424195943207 1104 16-17 1.6939595788007296 1104 18-19 0.874199768940219 1104 20-21 -3.661454823335543 1104 22-23 -0.30405420595826627 1104 24-25 1.1141083445108144 1104 26-27 1.323752397316035 1104 28-29 0.9334079923259857 1104 30-31 1.718335588500839 1104 32-33 1.5556839996316896 1104 34-35 1.811067238624318 1104 36-37 1.9692341865123204 1104 38-39 2.018333321398341 1104 40-41 2.0177207086980324 1104 42-43 2.004555331645811 1104 44-45 2.083112209819742 1104 46-47 2.0352929943910993 1104 48-49 2.0930898293563907 1104 50-51 1.9788267101113028 1104 52-53 2.085402219390385 1104 54-55 2.0177612324761363 1104 56-57 2.341917634925373 1104 58-59 2.2497876610755405 1104 60-61 2.300485490734303 1104 62-63 1.9861240955532864 1104 64-65 2.103511552065381 1104 66-67 2.079316448255753 1104 68-69 1.9144806367019278 1104 70-71 2.1686126820382725 1104 72-73 2.14777936972364 1104 74-75 2.1598862320930223 1104 76-77 2.0822915701896036 1104 78-79 2.020508229090506 1104 80-81 1.7745433378156577 1104 82-83 1.7628529050001163 1104 84-85 1.7579236807352778 1104 86-87 1.6430708342598699 1104 88-89 1.8443415080100678 1104 90-91 1.8368758877722726 1104 92-93 1.5405754514113497 1104 94-95 1.6221016807302213 1104 96-97 1.7307145073564918 1104 98-99 1.6290882026726905 1104 100-101 1.2531948343695802 1104 102-103 1.5820560862955766 1104 104-105 1.7456810573522326 1104 106-107 1.9231037484936095 1104 108-109 1.9394103623221461 1104 110-111 1.8973124477643886 1104 112-113 1.8635883674976164 1104 114-115 1.8158136157038172 1104 116-117 1.8971141684679402 1104 118-119 1.8566957017112884 1104 120-121 1.9060761561609283 1104 122-123 1.8032120305107213 1104 124-125 1.862329105806829 1105 1 1.599545621717379 1105 2 1.2399835749812453 1105 3 1.3386613400872598 1105 4 2.015527218774455 1105 5 1.6574951271550837 1105 6 1.5375305240121655 1105 7 1.4218082298121786 1105 8 1.6536761219704168 1105 9 2.14130351209608 1105 10-11 1.8843708400944195 1105 12-13 1.899609859072232 1105 14-15 2.315695871100303 1105 16-17 2.122988028014003 1105 18-19 1.7458741161739049 1105 20-21 1.9216095263873676 1105 22-23 1.7362147285227323 1105 24-25 1.7429846562665006 1105 26-27 1.708573369685645 1105 28-29 1.2202738951938343 1105 30-31 1.6520215073521278 1105 32-33 1.6494270396295931 1105 34-35 1.4703567124809105 1105 36-37 1.7794148681667359 1105 38-39 1.7361027799265578 1105 40-41 1.835556164422595 1105 42-43 2.157525123599065 1105 44-45 2.2959380198002215 1105 46-47 2.568689131508055 1105 48-49 2.1427817878518525 1105 50-51 2.3114750973559914 1105 52-53 2.282372735183859 1105 54-55 2.3918678957364996 1105 56-57 2.6255586003513613 1105 58-59 2.3575714817727835 1105 60-61 2.7446986174620207 1105 62-63 2.605956908114699 1105 64-65 2.4004268791363508 1105 66-67 2.550176557126562 1105 68-69 2.174985326924517 1105 70-71 2.2267544924903966 1105 72-73 2.172086153514858 1105 74-75 2.485729374935193 1105 76-77 2.315936793499226 1105 78-79 2.1851278363171183 1105 80-81 2.164502838791698 1105 82-83 2.1686468201568694 1105 84-85 2.1707693062511346 1105 86-87 2.113539135953854 1105 88-89 2.2865947800298763 1105 90-91 2.2672107703201902 1105 92-93 2.337765084447284 1105 94-95 2.174481837682361 1105 96-97 2.3342433103016376 1105 98-99 2.105067597791191 1105 100-101 2.209487249441178 1105 102-103 2.150851203485633 1105 104-105 2.215620398886905 1105 106-107 2.3977021973138477 1105 108-109 2.481388591435593 1105 110-111 2.3817879583828976 1105 112-113 2.4206759423381072 1105 114-115 2.4433916823626234 1105 116-117 2.5234660465221523 1105 118-119 2.6378879746350776 1105 120-121 2.5874689590274684 1105 122-123 2.635983241321817 1105 124-125 2.2515504371487864 1106 1 -0.2009430236066727 1106 2 0.027107225058749407 1106 3 0.012434507259964533 1106 4 -0.4078573738171265 1106 5 -0.9330883144524442 1106 6 -0.2757109837102547 1106 7 -0.280984979169709 1106 8 0.03841459479463438 1106 9 0.2580974554100415 1106 10-11 -2.2715110821126103 1106 12-13 -0.400044894970101 1106 14-15 -0.044391608386330716 1106 16-17 -0.21188586807232568 1106 18-19 -3.051709834691774 1106 20-21 -5.505618137015727 1106 22-23 -6.383555949953873 1106 24-25 -6.183359099429023 1106 26-27 -5.038911268261742 1106 28-29 -1.7354030733409083 1106 30-31 -3.4100319681642652 1106 32-33 -4.067797565951103 1106 34-35 -3.416465446538737 1106 36-37 -5.758710152436617 1106 38-39 -6.370954679852847 1106 40-41 -2.4197007229718643 1106 42-43 -0.98366338414192 1106 44-45 -0.14138618856669183 1106 46-47 0.019872484146972624 1106 48-49 0.32487359603279486 1106 50-51 0.2571875718694052 1106 52-53 0.05988835300228246 1106 54-55 0.13594994844092767 1106 56-57 0.33710624687012114 1106 58-59 0.5596841168692315 1106 60-61 0.6724142252013792 1106 62-63 0.4320538687453279 1106 64-65 0.21855735039338597 1106 66-67 -0.05327507831583489 1106 68-69 0.09870326762701254 1106 70-71 0.3353458366932074 1106 72-73 0.3353833897663101 1106 74-75 0.2721969856997859 1106 76-77 0.19922715817580539 1106 78-79 0.08044885651579747 1106 80-81 -0.31968600277104287 1106 82-83 -0.37408994225714665 1106 84-85 -0.4606730556912133 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-7.967477542552798 2216 102-103 -8.645176828437338 2216 104-105 -11.099641537970363 2216 106-107 -8.340790827900966 2216 108-109 -10.282314203152419 2216 110-111 -8.176605219903278 2216 112-113 -7.557287053377806 2216 114-115 -7.897152191011987 2216 116-117 -7.416978333124561 2216 118-119 -7.747146777131194 2216 120-121 -8.615385646550871 2216 122-123 -10.858355787307094 2216 124-125 -9.562465006487281 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 320938.0 3 46668.0 4 26906.0 5 22125.0 6 21946.0 7 24505.0 8 26381.0 9 27900.0 10 28589.0 11 29149.0 12 28598.0 13 27625.0 14 26387.0 15 22709.0 16 19527.0 17 17447.0 18 16571.0 19 16115.0 20 16127.0 21 18140.0 22 22600.0 23 30481.0 24 40906.0 25 51834.0 26 62178.0 27 71251.0 28 80870.0 29 90532.0 30 100542.0 31 113769.0 32 133213.0 33 158694.0 34 191458.0 35 233829.0 36 293047.0 37 265956.0 38 61157.0 39 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88741318649346 17.962668620700185 13.610094881976734 24.539823310829618 2 15.42398799630987 19.75314181512738 39.427204296057525 25.39566589250523 3 19.16158592952629 24.142090474277524 28.583055019150816 28.113268577045368 4 12.058367985569927 15.846549771765567 36.719570716771734 35.375511525892776 5 14.669511692467216 37.082275963194185 33.234741197438474 15.013471146900129 6 33.33383572669945 35.28383969201852 16.856015137829825 14.526309443452218 7 29.514321250454838 31.503736680472734 20.24349247747693 18.7384495915955 8 26.263599862346194 35.32704082712856 19.350515179059396 19.058844131465843 9 26.15293409211128 16.366550323585923 19.676155697329996 37.804359886972804 10-11 25.1920742729072 26.202606990243442 26.84299218348088 21.762326553368485 12-13 25.936507640306132 23.217175177088375 27.393012208771754 23.45330497383373 14-15 23.107204264068184 24.73529911030068 24.711936024622037 27.4455606010091 16-17 23.603140318726414 28.093750157023113 24.87588893028317 23.4272205939673 18-19 23.58034045054839 26.848731035883727 26.71606162981126 22.85486688375662 20-21 24.00820482526658 26.116400074523987 26.835085850808653 23.04030924940078 22-23 23.94673610126637 25.921907609154026 26.472204327220723 23.659151962358887 24-25 23.7979367746263 26.237925354731818 26.37683390782724 23.58730396281464 26-27 23.643306949851286 26.52347383470562 26.136083072082318 23.69713614336078 28-29 23.604497960895927 26.49747967131031 26.271777791214905 23.62624457657886 30-31 23.494322861101452 26.177493618803293 26.529791657022656 23.7983918630726 32-33 23.49565151860418 26.401463429513694 26.212864758370852 23.89002029351128 34-35 23.827361212799865 26.095618530800923 26.410751970369095 23.666268286030117 36-37 23.734403993840726 26.074473273140082 26.267300536729763 23.923822196289436 38-39 23.70865221675867 26.159141101564447 26.455583363777237 23.67662331789965 40-41 23.77161133077159 26.103615932573902 26.403953529693652 23.720819206960854 42-43 23.709845459592714 26.225483071112414 26.094525140226192 23.97014632906868 44-45 23.74879763325726 26.181750250801798 26.206915753047568 23.86253636289337 46-47 23.761665637655735 26.38109505064582 25.973958397814727 23.883280913883716 48-49 23.898142559075435 26.30061775934301 25.804892263545735 23.996347418035814 50-51 23.70733447432254 26.51452320874683 25.784639423844713 23.993502893085914 52-53 24.129403941139508 26.238519006062543 25.71638567024098 23.915691382556965 54-55 23.887175077659816 26.161757064028436 25.744443232255954 24.2066246260558 56-57 23.641338380784468 26.525871297173172 25.946424002402914 23.88636631963945 58-59 23.922642198094515 26.076873301782204 25.845874409672486 24.154610090450788 60-61 23.847912517536496 26.0290874776178 26.14380492577914 23.97919507906656 62-63 23.863018142617424 26.318799190981945 25.827553265857745 23.990629400542886 64-65 23.72694534708254 26.106973230674825 25.872959195574513 24.293122226668125 66-67 23.626850406416537 26.45473793759308 25.686888805153323 24.231522850837056 68-69 23.611845038743905 26.201231443343826 25.961492050703956 24.225431467208313 70-71 23.421094607738734 26.13036816888404 26.25919578909519 24.189341434282035 72-73 23.639781457159206 26.16280978731169 25.929382120014832 24.26802663551427 74-75 23.601120528190563 26.31278367788098 25.90671511308181 24.179380680846652 76-77 23.709282842604765 26.14797900358541 26.05485379701432 24.087884356795502 78-79 23.58470115197888 26.232149387940563 26.054168166592433 24.128981293488128 80-81 23.57591765543269 26.476429572747932 25.938764777662886 24.00888799415649 82-83 23.643873817281484 26.29306640269299 25.849055215749857 24.21400456427567 84-85 23.58601791979066 26.27143869863069 25.788519054198783 24.354024327379868 86-87 23.641499765311792 26.198506751412793 26.001479899349622 24.158513583925796 88-89 23.536015809853993 26.124692795007853 25.967441160274475 24.37185023486368 90-91 23.453619808422506 26.30069118760032 26.092610023565904 24.15307898041127 92-93 23.592613458978214 26.203452841902042 26.016977130574265 24.18695656854548 94-95 23.557766234353444 26.033834940594986 26.02820098716194 24.38019783788963 96-97 23.68587302398978 25.99325548160329 25.962627939961408 24.358243554445526 98-99 23.341266284109924 25.9449116855164 26.015927862779094 24.69789416759459 100-101 23.274514532273727 25.917162056544424 26.2157237213702 24.592599689811657 102-103 23.39263453203643 25.789454608871893 26.366258853554015 24.451652005537667 104-105 23.472809120450805 25.906618334023822 26.238861892218303 24.381710653307067 106-107 23.229940909216502 25.939964022335865 26.378022405479683 24.452072662967947 108-109 23.257230138436917 25.815165173543598 26.201012982823357 24.726591705196128 110-111 23.044052497473253 25.85852409304025 26.504631934736828 24.59279147474967 112-113 23.25146847706319 25.954880159675852 26.3351761104884 24.458475252772555 114-115 23.14215502803871 26.02655199748231 26.262011143714588 24.56928183076439 116-117 22.754844395831224 25.958938815526466 26.55602374487821 24.7301930437641 118-119 23.100815668880777 26.006667456139404 26.145507002982054 24.74700987199776 120-121 22.998263103876436 25.925112503498916 26.38201666559008 24.694607727034573 122-123 22.997653448970677 25.89791731484801 26.386982473176506 24.717446763004805 124-125 23.02526915867248 25.97223663344048 26.174983026676575 24.82751118121047 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 384.0 1 440.5 2 570.0 3 630.0 4 538.5 5 425.0 6 408.5 7 477.5 8 595.5 9 650.5 10 672.5 11 706.5 12 753.5 13 859.5 14 982.0 15 1105.0 16 1194.0 17 1282.5 18 1453.0 19 1703.5 20 2091.0 21 2572.0 22 3230.5 23 4112.0 24 5408.5 25 7130.0 26 9282.0 27 12082.0 28 15035.5 29 18188.5 30 22085.5 31 26435.0 32 30830.0 33 35217.5 34 39680.5 35 43711.5 36 48133.5 37 53550.0 38 58931.0 39 63047.5 40 66855.5 41 72882.0 42 78832.0 43 84621.0 44 90793.0 45 95096.5 46 98508.0 47 102205.5 48 108182.5 49 112736.0 50 113644.0 51 114192.5 52 114317.5 53 114574.0 54 112823.5 55 107774.0 56 101543.5 57 94674.0 58 87904.5 59 79571.0 60 68972.0 61 57291.5 62 45724.5 63 35535.5 64 27139.5 65 20543.5 66 15519.0 67 12050.5 68 9171.0 69 6861.5 70 5154.5 71 3847.5 72 2836.5 73 2179.5 74 1695.0 75 1263.0 76 910.5 77 605.0 78 385.0 79 247.0 80 173.5 81 125.5 82 77.0 83 52.0 84 47.5 85 41.0 86 36.0 87 31.5 88 19.5 89 14.0 90 16.0 91 14.0 92 8.0 93 4.0 94 5.5 95 3.5 96 3.5 97 3.5 98 2.5 99 4.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09914782391518716 2 0.03365930468058109 3 0.04402981539773241 4 0.05217551066691355 5 0.05511800851745475 6 0.0031219184511839604 7 0.0017942060064275634 8 0.0016865536460419098 9 0.005813227460825306 10-11 0.044388656599017924 12-13 0.005203197418639935 14-15 0.010801120158693932 16-17 0.01916212014864638 18-19 0.017906175944147083 20-21 0.1346192766622601 22-23 0.015537824015662699 24-25 0.013725675949170861 26-27 0.005597922740053998 28-29 0.0038575429138192614 30-31 0.0016147854057848073 32-33 0.005131429178382832 34-35 0.016793768220161997 36-37 0.0020453948473274225 38-39 0.0072485922659673565 40-41 0.03168567807351077 42-43 0.012810630885892805 44-45 0.11395002346821456 46-47 0.0032833969917624413 48-49 0.008109811149052588 50-51 0.009939901275608704 52-53 0.010585815437922625 54-55 0.0084686523503381 56-57 0.004557283256326012 58-59 0.013313008567692521 60-61 0.02772048279930586 62-63 0.005741459220568203 64-65 0.005849111580953858 66-67 0.007105055785453152 68-69 0.006477083683203504 70-71 0.0028169034300912746 72-73 6.279721022496473E-4 74-75 9.509291834066087E-4 76-77 0.006351489262753574 78-79 0.017906175944147083 80-81 0.002422178108677211 82-83 0.0038216587936907107 84-85 0.0018839163067489416 86-87 0.0025836566492556915 88-89 0.004718761796904492 90-91 0.003408991412212371 92-93 0.0020812789674559736 94-95 0.0026015987093199675 96-97 0.0014533068652063264 98-99 0.002117163087584525 100-101 0.0019377424869417687 102-103 5.023776817997178E-4 104-105 0.0031398605112482364 106-107 6.817982824424741E-4 108-109 9.8681330353516E-4 110-111 0.0022068733879059034 112-113 0.003678122313176505 114-115 0.0023324678083558328 116-117 0.00452139913619746 118-119 0.002798961370026999 120-121 0.006028532181596614 122-123 0.003767832613497883 124-125 0.05364675959218415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2786748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.17339747499673 #Duplication Level Percentage of deduplicated Percentage of total 1 78.91485657003606 46.696601745011016 2 10.475679832054466 12.397631330459319 3 4.18340324066852 7.4263854827480325 4 2.0992679416419318 4.968832652691851 5 1.2018969500906265 3.5560162975849483 6 0.723700453577219 2.569428875541612 7 0.4983816613717223 2.064365529981871 8 0.3465396364931702 1.6404742120840996 9 0.25375846529042856 1.3514175476348125 >10 1.2228512537252623 12.423111563418269 >50 0.0558884986194791 2.248296077416573 >100 0.022886617796649845 2.301500716935032 >500 8.253873034679281E-4 0.3054814884788726 >1k 6.34913310293162E-5 0.05045648001368834 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.0765236038565382E-4 0.0 0.0 0.0 6 0.0 1.0765236038565382E-4 0.0 0.0 7.176824025710254E-5 7 0.0 1.0765236038565382E-4 0.0 0.0 7.176824025710254E-5 8 0.0 1.0765236038565382E-4 0.0 3.588412012855127E-5 7.176824025710254E-5 9 0.0 1.0765236038565382E-4 0.0 3.588412012855127E-5 7.176824025710254E-5 10-11 0.0 1.0765236038565382E-4 0.0 7.176824025710254E-5 7.176824025710254E-5 12-13 0.0 1.0765236038565382E-4 0.0 7.176824025710254E-5 1.0765236038565382E-4 14-15 0.0 1.0765236038565382E-4 0.0 1.0765236038565382E-4 1.2559442044992946E-4 16-17 0.0 1.0765236038565382E-4 0.0 1.7942060064275636E-4 1.4353648051420507E-4 18-19 0.0 1.0765236038565382E-4 0.0 2.3324678083558328E-4 1.7942060064275636E-4 20-21 0.0 1.0765236038565382E-4 0.0 2.6913090096413454E-4 1.7942060064275636E-4 22-23 0.0 1.4353648051420507E-4 0.0 4.126673814783396E-4 1.97362660707032E-4 24-25 0.0 1.4353648051420507E-4 0.0 6.638562223781985E-4 2.1530472077130764E-4 26-27 0.0 1.4353648051420507E-4 0.0 0.0010406394837279868 2.1530472077130764E-4 28-29 0.0 1.4353648051420507E-4 0.0 0.0019198004268774931 2.1530472077130764E-4 30-31 0.0 1.4353648051420507E-4 0.0 0.004180499994976224 2.1530472077130764E-4 32-33 0.0 1.4353648051420507E-4 0.0 0.008971030032137818 2.1530472077130764E-4 34-35 0.0 1.4353648051420507E-4 0.0 0.015735186676369733 2.511888408998589E-4 36-37 0.0 1.6147854057848072E-4 0.0 0.026661901255513595 2.511888408998589E-4 38-39 0.0 2.3324678083558328E-4 0.0 0.04618286260544549 2.511888408998589E-4 40-41 0.0 2.511888408998589E-4 0.0 0.06943577244874671 2.6913090096413454E-4 42-43 0.0 2.511888408998589E-4 0.0 0.09143273808754865 2.8707296102841015E-4 44-45 0.0 2.6913090096413454E-4 0.0 0.11768197196158389 2.8707296102841015E-4 46-47 0.0 2.8707296102841015E-4 0.0 0.1508388989603653 3.2295708115696143E-4 48-49 0.0 2.8707296102841015E-4 0.0 0.1888940083566939 3.588412012855127E-4 50-51 0.0 2.8707296102841015E-4 0.0 0.23016074650452786 3.588412012855127E-4 52-53 0.0 2.8707296102841015E-4 0.0 0.2711404116913334 4.126673814783396E-4 54-55 0.0 3.050150210926858E-4 0.0 0.3170900275159433 4.844356217354422E-4 56-57 0.0 3.2295708115696143E-4 0.0 0.37161594805127696 5.203197418639934E-4 58-59 0.0 3.2295708115696143E-4 0.0 0.4356870445408053 5.562038619925447E-4 60-61 0.0 3.2295708115696143E-4 0.0 0.5073835165576507 5.741459220568203E-4 62-63 0.0 4.6649356167116656E-4 0.0 0.5810536151815664 6.459141623139229E-4 64-65 0.0 4.844356217354422E-4 0.0 0.6562129047908172 6.817982824424741E-4 66-67 0.0 5.382618019282691E-4 0.0 0.7494757330049219 7.176824025710254E-4 68-69 0.0 5.382618019282691E-4 0.0 0.8539702908192632 7.176824025710254E-4 70-71 0.0 5.741459220568203E-4 0.0 0.9757430524755019 7.535665226995767E-4 72-73 0.0 5.741459220568203E-4 0.0 1.1033828677727588 8.612188830852306E-4 74-75 0.0 5.741459220568203E-4 0.0 1.2344675586023566 8.612188830852306E-4 76-77 0.0 6.100300421853716E-4 0.0 1.3819154082105738 8.791609431495061E-4 78-79 0.0 6.459141623139229E-4 0.0 1.5496557277514866 8.971030032137818E-4 80-81 0.0 7.176824025710254E-4 0.0 1.7398953906130012 9.150450632780575E-4 82-83 0.0 7.715085827638523E-4 0.0 1.942712437579573 0.0010047553635994357 84-85 0.0 8.073927028924037E-4 0.0 2.151522132607613 0.0010047553635994357 86-87 0.0 8.253347629566792E-4 0.0 2.3712944263349254 0.0010047553635994357 88-89 0.0 8.612188830852306E-4 0.0 2.6100135354901126 0.0010585815437922624 90-91 3.588412012855127E-5 8.612188830852306E-4 0.0 2.8881154664863846 0.0010765236038565381 92-93 3.588412012855127E-5 8.791609431495061E-4 0.0 3.19426083736312 0.001094465663920814 94-95 3.588412012855127E-5 9.329871233423331E-4 0.0 3.501052122402169 0.001184175964242192 96-97 3.588412012855127E-5 9.329871233423331E-4 0.0 3.816096755070785 0.001184175964242192 98-99 3.588412012855127E-5 9.688712434708844E-4 0.0 4.15170298857306 0.001184175964242192 100-101 3.588412012855127E-5 9.868133035351601E-4 0.0 4.533043533179175 0.001184175964242192 102-103 3.588412012855127E-5 0.0010406394837279868 0.0 4.943037547707938 0.0012200600843707433 104-105 3.588412012855127E-5 0.0010765236038565381 0.0 5.361715519307809 0.0012200600843707433 106-107 3.588412012855127E-5 0.0011124077239850895 0.0 5.782008276313466 0.0012380021444350188 108-109 3.588412012855127E-5 0.0011124077239850895 0.0 6.225607769342617 0.0013097703846921215 110-111 3.588412012855127E-5 0.0011482918441136406 0.0 6.709971622837802 0.0014533068652063264 112-113 3.588412012855127E-5 0.0011482918441136406 0.0 7.240787469839397 0.0014712489252706022 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5240 0.0 53.502155 1 ATCAACG 6095 0.0 42.662 3 TCAACGC 6115 0.0 42.661896 4 GGTATCA 2925 0.0 42.32716 1 TATCAAC 6700 0.0 41.028442 2 CAACGCA 6295 0.0 40.87432 5 AACGCAG 6325 0.0 40.189384 6 ACGCAGA 7400 0.0 34.109722 7 CGCAGAG 7460 0.0 32.960537 8 GTACATG 8540 0.0 32.061337 1 TACATGG 8985 0.0 29.79973 2 GCAGAGT 8170 0.0 28.347229 9 ACATGGG 9315 0.0 26.956474 3 AGAGTAC 7515 0.0 23.360445 10-11 TATACCG 265 3.6907295E-8 22.475876 5 CAGAGTA 8190 0.0 21.217148 10-11 GAGTACA 6710 0.0 20.225254 1 GTATAGG 630 0.0 19.840857 1 CATGGGA 5170 0.0 19.584879 4 CATGGGG 5775 0.0 19.389578 4 >>END_MODULE