FastQCFastQC Report
Thu 26 May 2016
SRR1364480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786748
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40710.14608425304333222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38180.13700557065080876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.061.0307431
GTATCAA71150.050.582911
TCAACGC88250.039.0429884
ATCAACG90050.038.725953
CAACGCA90000.038.2061735
TATCAAC94950.037.60642
AACGCAG91950.037.0077326
GTACATG89250.030.6948721
ACGCAGA109700.030.6400877
CGCAGAG112450.029.8271668
TACATGG94050.028.268712
GCAGAGT122300.026.3551279
ACATGGG93750.025.1798883
GAGTACT73350.023.87901912-13
GTACTTT77950.022.81320614-15
CAGAGTA120100.022.70531810-11
GTGGTAT22350.022.6987951
CATGGGG55000.022.6523634
AGAGTAC115450.022.61527410-11
AGTACTT76150.022.61048712-13
Produced by FastQC (version 0.11.5)