##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364480_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2786748 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.994229115800927 31.0 28.0 31.0 25.0 34.0 2 29.164891838085108 31.0 28.0 33.0 25.0 34.0 3 29.25933166543943 31.0 28.0 34.0 25.0 34.0 4 32.71706914295803 35.0 32.0 37.0 26.0 37.0 5 31.904822036294636 35.0 32.0 37.0 22.0 37.0 6 31.958001225801542 35.0 32.0 37.0 23.0 37.0 7 31.454869977479127 35.0 32.0 37.0 18.0 37.0 8 31.539438801068485 35.0 32.0 37.0 18.0 37.0 9 32.1194166103286 37.0 31.0 39.0 15.0 39.0 10-11 32.309291152267804 37.0 31.5 39.0 17.0 39.0 12-13 32.08675093693438 37.0 30.5 39.0 15.0 39.0 14-15 33.046137289772886 37.5 31.0 40.0 16.5 41.0 16-17 33.081204149065506 38.0 31.5 40.0 17.0 41.0 18-19 32.89217808714674 38.0 31.0 40.0 15.5 41.0 20-21 32.777218105117505 37.5 31.0 40.0 13.0 41.0 22-23 32.75057271055725 37.0 31.0 40.0 13.0 41.0 24-25 32.56058154522763 37.0 31.0 40.0 10.0 41.0 26-27 32.62425719871334 37.0 31.0 40.0 10.5 41.0 28-29 32.49101694878762 37.0 30.5 40.0 10.0 41.0 30-31 32.015981172319854 37.0 30.0 40.0 9.0 41.0 32-33 31.960048056013676 37.0 30.0 40.0 8.5 41.0 34-35 31.771196211498133 37.0 30.0 40.0 8.0 41.0 36-37 31.74940522070887 37.0 30.0 40.0 8.0 41.0 38-39 31.515899715367162 37.0 30.0 40.0 4.5 41.0 40-41 31.23786883492874 36.0 29.5 40.0 2.0 41.0 42-43 31.125674083196614 36.0 29.0 40.0 2.0 41.0 44-45 31.056699601112122 36.0 29.0 40.0 2.0 41.0 46-47 30.92301438809681 36.0 29.0 40.0 2.0 41.0 48-49 30.6659780504014 36.0 28.0 40.0 2.0 41.0 50-51 30.31491455273315 36.0 28.0 40.0 2.0 41.0 52-53 30.191188080156515 35.0 28.0 40.0 2.0 41.0 54-55 29.699768152699846 35.0 26.5 39.0 2.0 41.0 56-57 29.7012067470758 35.0 27.0 39.0 2.0 41.0 58-59 29.45024002887954 35.0 26.5 39.0 2.0 41.0 60-61 29.13693595545776 34.0 26.0 39.0 2.0 40.5 62-63 28.850595748162377 34.0 26.0 38.5 2.0 40.0 64-65 28.361274503471428 34.0 25.0 38.0 2.0 40.0 66-67 27.965163337338 34.0 24.0 37.0 2.0 40.0 68-69 27.770504724503255 34.0 23.5 37.0 2.0 40.0 70-71 27.829515801213457 34.0 25.5 36.5 2.0 39.0 72-73 27.60772610225252 34.0 25.0 36.0 2.0 39.0 74-75 27.259592901833965 33.0 24.5 36.0 2.0 39.0 76-77 27.180878213602377 33.0 25.0 35.0 2.0 37.5 78-79 26.81363887226258 33.0 24.0 35.0 2.0 37.0 80-81 26.598087268744788 33.0 24.0 35.0 2.0 37.0 82-83 26.466882007271558 33.0 24.0 35.0 2.0 36.0 84-85 26.080484134195125 33.0 22.0 35.0 2.0 36.0 86-87 25.96882244106751 33.0 21.5 35.0 2.0 35.5 88-89 25.941248006637124 33.0 23.0 35.0 2.0 35.0 90-91 25.8031486880048 33.0 21.5 35.0 2.0 35.0 92-93 25.821663099785127 33.0 23.0 35.0 2.0 35.0 94-95 25.833372805865476 33.0 23.0 35.0 2.0 35.0 96-97 25.72156937046335 33.0 23.0 35.0 2.0 35.0 98-99 25.59919572921556 33.0 20.0 35.0 2.0 35.0 100-101 25.513328079898145 33.0 20.0 35.0 2.0 35.0 102-103 24.34472941220376 30.5 18.5 34.0 2.0 35.0 104-105 25.31428335106009 32.0 20.0 35.0 2.0 35.0 106-107 25.438944604966075 33.0 20.0 35.0 2.0 35.0 108-109 25.510750702969915 33.0 20.0 35.0 2.0 35.0 110-111 25.447877956672077 33.0 20.0 35.0 2.0 35.0 112-113 25.39515736622041 33.0 19.5 35.0 2.0 35.0 114-115 25.337247034895153 33.0 19.0 35.0 2.0 35.0 116-117 25.249834574206208 33.0 19.0 35.0 2.0 35.0 118-119 25.12863864978104 33.0 18.0 35.0 2.0 35.0 120-121 24.953388322159018 33.0 12.5 35.0 2.0 35.0 122-123 24.644538006306995 33.0 2.0 35.0 2.0 35.0 124-125 23.845037297954462 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.876750351540796 1101 2 0.8500757851842877 1101 3 0.8935264958597209 1101 4 0.9359852625339116 1101 5 1.1492771618080262 1101 6 1.26579520699471 1101 7 1.6172562829725123 1101 8 1.34826480499542 1101 9 2.062774743782775 1101 10-11 1.7642458226512545 1101 12-13 1.941024276412417 1101 14-15 1.8389833984245385 1101 16-17 1.9013944179990787 1101 18-19 1.9642568557909428 1101 20-21 2.1702056922187225 1101 22-23 2.1504324031893987 1101 24-25 2.2998298768623755 1101 26-27 1.9955417576986392 1101 28-29 2.021472333619485 1101 30-31 2.4908646964922596 1101 32-33 2.3798283444668513 1101 34-35 2.508243968484802 1101 36-37 2.2795547842550015 1101 38-39 2.4077224559738397 1101 40-41 2.5278445860260526 1101 42-43 2.4432627883076954 1101 44-45 2.2338628466765726 1101 46-47 1.9960180005019907 1101 48-49 2.1389286603865223 1101 50-51 2.4163028169866223 1101 52-53 2.2942276345520014 1101 54-55 2.6289486716744612 1101 56-57 2.4657976728311546 1101 58-59 2.550069399860398 1101 60-61 2.56837378569028 1101 62-63 2.5840758326268976 1101 64-65 2.687890476266954 1101 66-67 2.8620006917381566 1101 68-69 2.7303573702082318 1101 70-71 2.3398956258042176 1101 72-73 2.5173129764186264 1101 74-75 2.6452377125800943 1101 76-77 2.467819226142634 1101 78-79 2.5806938243820667 1101 80-81 2.5964886504602234 1101 82-83 2.375605628000521 1101 84-85 2.6760221431602282 1101 86-87 2.651226143534224 1101 88-89 2.54951183177684 1101 90-91 2.4667663503967603 1101 92-93 2.3162121982233046 1101 94-95 2.315305204702316 1101 96-97 2.1975875706226766 1101 98-99 2.329840468585605 1101 100-101 2.4280016998518725 1101 102-103 2.1909044625911775 1101 104-105 2.24950491652298 1101 106-107 2.3054309014139847 1101 108-109 2.290331149044164 1101 110-111 2.2997213780795036 1101 112-113 2.1924485244888032 1101 114-115 2.202724003830518 1101 116-117 2.249034365386475 1101 118-119 2.1406589322479697 1101 120-121 2.087660386523872 1101 122-123 2.1808432343771997 1101 124-125 2.021391683629389 1102 1 0.5892880403667959 1102 2 -1.5680998316122334 1102 3 -0.01117074360259096 1102 4 0.4341754723991542 1102 5 1.0547972871660996 1102 6 0.6110093920386959 1102 7 1.3720754976888259 1102 8 1.1028072096514911 1102 9 1.833015315196235 1102 10-11 1.6480535424851936 1102 12-13 1.7970166833224575 1102 14-15 1.8585120128728292 1102 16-17 1.8355745833474444 1102 18-19 1.894913700357428 1102 20-21 2.1147539404357616 1102 22-23 2.011592598722359 1102 24-25 2.220721110254239 1102 26-27 1.933470594414608 1102 28-29 1.933078042463741 1102 30-31 2.2630241707543277 1102 32-33 1.8991639742432653 1102 34-35 2.151888551073487 1102 36-37 1.4616864433904162 1102 38-39 1.4692862428099787 1102 40-41 1.7024878903707084 1102 42-43 1.5408700484804747 1102 44-45 1.5278603248363254 1102 46-47 1.1043320446820637 1102 48-49 1.2259133810672864 1102 50-51 1.4455414384352636 1102 52-53 1.2927402927444795 1102 54-55 1.6078517307795543 1102 56-57 1.205058057213403 1102 58-59 1.2142608425176853 1102 60-61 1.2371841935250139 1102 62-63 1.5838957943205578 1102 64-65 1.626337912718931 1102 66-67 1.6715340879591594 1102 68-69 1.7025205886709784 1102 70-71 1.0416657572163466 1102 72-73 1.3245825748875681 1102 74-75 1.5787879767045787 1102 76-77 1.5264265381008677 1102 78-79 1.4173669008967593 1102 80-81 1.4723475347033208 1102 82-83 1.13295813909248 1102 84-85 1.1495152048396982 1102 86-87 1.2174239841177084 1102 88-89 1.4025214985206276 1102 90-91 1.1829618066136405 1102 92-93 1.2042657671171568 1102 94-95 1.2775116901049621 1102 96-97 1.2924076366323796 1102 98-99 1.0773224470584992 1102 100-101 0.9993527993242957 1102 102-103 0.8210542753252597 1102 104-105 1.1121411374360335 1102 106-107 1.3079036106617146 1102 108-109 1.2178441224201038 1102 110-111 1.116618508680581 1102 112-113 1.2099904306632325 1102 114-115 1.422511471078078 1102 116-117 1.0630004947137728 1102 118-119 0.6487899017798604 1102 120-121 0.9949976600230137 1102 122-123 1.2770426471524132 1102 124-125 1.2210855067414315 1103 1 0.7313577893284773 1103 2 0.6685765818753673 1103 3 0.5002894300247114 1103 4 0.6501828197698742 1103 5 0.9141670570435458 1103 6 0.887655714060493 1103 7 1.2021153548867431 1103 8 1.1427936654660975 1103 9 1.880007322197912 1103 10-11 1.5111037584697513 1103 12-13 1.5149927315595697 1103 14-15 1.2752841093546365 1103 16-17 1.2034408143762292 1103 18-19 1.3915020293619946 1103 20-21 1.7303099820085777 1103 22-23 1.5579557145128575 1103 24-25 1.8992248030398926 1103 26-27 1.262805111965882 1103 28-29 1.4869242639537177 1103 30-31 2.1442787590787944 1103 32-33 1.8850444162655648 1103 34-35 1.9303102544486386 1103 36-37 1.8227604413154843 1103 38-39 1.9207234733966132 1103 40-41 2.1616787983303887 1103 42-43 2.0538825933992406 1103 44-45 2.015887138238611 1103 46-47 1.940291951472652 1103 48-49 1.859281388044753 1103 50-51 2.051163154745659 1103 52-53 1.9323475227146112 1103 54-55 2.11845877390725 1103 56-57 1.9171686618831778 1103 58-59 2.004621219591435 1103 60-61 2.03130193792078 1103 62-63 2.1405301380024646 1103 64-65 2.1538954225555784 1103 66-67 2.4105620591037287 1103 68-69 2.5070239735826583 1103 70-71 2.2152538203297567 1103 72-73 2.2869220993622257 1103 74-75 2.3163906123932243 1103 76-77 2.2944234075445245 1103 78-79 2.3649333443737106 1103 80-81 2.3092759774548384 1103 82-83 2.1545028872882597 1103 84-85 2.3759686612167137 1103 86-87 2.2959094370725595 1103 88-89 2.1986481300351954 1103 90-91 2.2344960893637342 1103 92-93 2.086194349811386 1103 94-95 1.9245389097193346 1103 96-97 1.9441697896169963 1103 98-99 2.0527007557108554 1103 100-101 2.017746141606395 1103 102-103 1.8113373340493837 1103 104-105 1.8470034489341458 1103 106-107 1.9282016300184388 1103 108-109 1.9048078250622638 1103 110-111 1.8828019380259597 1103 112-113 1.8609870591784912 1103 114-115 1.7350619875612523 1103 116-117 1.8170727331428758 1103 118-119 1.8449607476276668 1103 120-121 1.7995268114141751 1103 122-123 1.9936164788482245 1103 124-125 1.7775836426666523 1104 1 0.7081968388861597 1104 2 0.6167712983685654 1104 3 0.61039556104339 1104 4 0.625223146996511 1104 5 1.039163856470001 1104 6 0.9946284835807973 1104 7 1.4268716312114549 1104 8 1.2134215219779811 1104 9 1.7074684032236291 1104 10-11 1.2917540510021794 1104 12-13 1.4274510440891781 1104 14-15 1.4504018904610092 1104 16-17 1.2938430144504807 1104 18-19 1.3330087473658594 1104 20-21 1.5872459449331373 1104 22-23 1.5493773772748227 1104 24-25 1.6674964773239225 1104 26-27 1.5629172920685548 1104 28-29 1.4733530319889496 1104 30-31 2.035413650451325 1104 32-33 1.9698472129267302 1104 34-35 1.895222366699155 1104 36-37 1.799893943639102 1104 38-39 1.8885537085747544 1104 40-41 2.0690489990703647 1104 42-43 1.965359930137982 1104 44-45 1.7010771242841116 1104 46-47 1.8612893942143955 1104 48-49 1.6214859751067543 1104 50-51 2.046920794514712 1104 52-53 1.7334678834832644 1104 54-55 2.0369885744475056 1104 56-57 1.8934792167346082 1104 58-59 2.029238430995722 1104 60-61 2.1503132647505474 1104 62-63 2.0493938063718353 1104 64-65 2.20715616688096 1104 66-67 2.131632874993933 1104 68-69 2.3902226427841775 1104 70-71 2.1974470293528086 1104 72-73 2.310715163079241 1104 74-75 2.411715694647963 1104 76-77 2.2214983393723067 1104 78-79 2.239693701429694 1104 80-81 2.1730619679043244 1104 82-83 2.1080813685521065 1104 84-85 2.3028971668049145 1104 86-87 2.2213172701562485 1104 88-89 2.0013759788657097 1104 90-91 2.0165242288063254 1104 92-93 1.7928949905662108 1104 94-95 1.7999609853552876 1104 96-97 1.9079969074365088 1104 98-99 1.8724836608074469 1104 100-101 1.8768416915289414 1104 102-103 1.8027461945171765 1104 104-105 1.6914595754720203 1104 106-107 1.7999642498799204 1104 108-109 1.7511790947937982 1104 110-111 1.7586812956068414 1104 112-113 1.7578201716722504 1104 114-115 1.6372128529963348 1104 116-117 1.6784999008913353 1104 118-119 1.705394537362789 1104 120-121 1.6936382361814921 1104 122-123 1.8512717706765223 1104 124-125 1.5877552254142806 1105 1 0.4110735305760791 1105 2 0.47814688955932994 1105 3 0.40639708078031944 1105 4 0.6047116114071542 1105 5 0.9068370213221577 1105 6 0.9650427429055597 1105 7 1.2275691053038713 1105 8 1.1912715200128083 1105 9 1.7616393277739277 1105 10-11 1.2735548737563036 1105 12-13 1.577571329301879 1105 14-15 1.4175326664439254 1105 16-17 1.381770358579189 1105 18-19 1.6420987557506166 1105 20-21 1.786415002565228 1105 22-23 1.7490680256522353 1105 24-25 1.7759083358573733 1105 26-27 1.6230951078397666 1105 28-29 1.6188834005251564 1105 30-31 2.00721909137156 1105 32-33 1.9265711481282892 1105 34-35 2.1131736041056506 1105 36-37 1.736718414023887 1105 38-39 1.7770192655714467 1105 40-41 1.9130970509248293 1105 42-43 1.8971506813596726 1105 44-45 1.9055030305582576 1105 46-47 1.714399368798098 1105 48-49 1.716506380195252 1105 50-51 1.825148925369298 1105 52-53 1.8523553639952546 1105 54-55 2.098652964784076 1105 56-57 1.8610582987348963 1105 58-59 1.828143499842426 1105 60-61 1.6668940368031109 1105 62-63 1.5331974720821187 1105 64-65 2.0220168358272304 1105 66-67 2.199976001459632 1105 68-69 2.131265120086386 1105 70-71 2.1283984862040732 1105 72-73 2.2575472976545683 1105 74-75 2.3107899324867915 1105 76-77 2.0803593227469115 1105 78-79 2.29291274334037 1105 80-81 2.2377744297471374 1105 82-83 2.1510593814960686 1105 84-85 2.3586566094807004 1105 86-87 2.2493836833150738 1105 88-89 2.118151712859337 1105 90-91 2.1772789878101086 1105 92-93 1.928077473632932 1105 94-95 1.8720023294053867 1105 96-97 1.903499927258597 1105 98-99 1.9706319727203514 1105 100-101 1.9836924625334085 1105 102-103 1.8083002761899394 1105 104-105 1.450303691076833 1105 106-107 1.6562180983516654 1105 108-109 1.7062047894838912 1105 110-111 1.791022378288389 1105 112-113 1.9120018228140196 1105 114-115 1.807559723124335 1105 116-117 1.8124226235786516 1105 118-119 1.9180934296579828 1105 120-121 1.934220943933461 1105 122-123 2.054367088314642 1105 124-125 1.9337418425584865 1106 1 0.509164077736834 1106 2 0.39160682128690993 1106 3 0.4454382166381947 1106 4 0.3673098775801762 1106 5 0.5646756940984012 1106 6 0.635040048838988 1106 7 0.9886195146617922 1106 8 0.6489285422242652 1106 9 1.3506938581822112 1106 10-11 1.0166601378714475 1106 12-13 0.8593748136279764 1106 14-15 1.0646240123518425 1106 16-17 1.0168049040151388 1106 18-19 1.0495774618256846 1106 20-21 0.924083130696701 1106 22-23 0.8766688797963837 1106 24-25 0.6662835556738784 1106 26-27 0.765268550784846 1106 28-29 0.7215428250407072 1106 30-31 1.0861376225710089 1106 32-33 0.8485621485818484 1106 34-35 0.928720509093008 1106 36-37 0.8704947811111019 1106 38-39 1.073659772131741 1106 40-41 1.3688929505630831 1106 42-43 1.3362025404361226 1106 44-45 1.2972451291944367 1106 46-47 1.1981849529274342 1106 48-49 1.194278057484727 1106 50-51 1.5797704715361647 1106 52-53 1.223429027202073 1106 54-55 1.7131111978477378 1106 56-57 1.4764889628961946 1106 58-59 1.5356032034647882 1106 60-61 1.6703477278448275 1106 62-63 1.630385213283489 1106 64-65 1.7277443891562996 1106 66-67 1.844028707405105 1106 68-69 1.6874795567972285 1106 70-71 1.562269571897744 1106 72-73 1.5661607229726542 1106 74-75 1.7946575511327651 1106 76-77 1.3730555415430068 1106 78-79 1.3508812598948623 1106 80-81 1.4578435774558756 1106 82-83 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-6.236531719760734 2216 104-105 -6.130618184105742 2216 106-107 -6.323374998459329 2216 108-109 -6.3975292726645705 2216 110-111 -6.1243665249409815 2216 112-113 -6.180746235644158 2216 114-115 -6.027648030614547 2216 116-117 -5.833057110665674 2216 118-119 -6.080234842016591 2216 120-121 -5.9624041698609105 2216 122-123 -6.114307444902693 2216 124-125 -6.025471637812583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126797.0 3 42603.0 4 21612.0 5 17693.0 6 18026.0 7 21516.0 8 23882.0 9 25200.0 10 27200.0 11 28879.0 12 30741.0 13 31811.0 14 31414.0 15 25763.0 16 19437.0 17 17148.0 18 15722.0 19 14721.0 20 14643.0 21 16028.0 22 20132.0 23 27284.0 24 37544.0 25 49751.0 26 60695.0 27 70313.0 28 79470.0 29 90271.0 30 100964.0 31 115372.0 32 133512.0 33 157424.0 34 190295.0 35 240058.0 36 336774.0 37 393721.0 38 112077.0 39 255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.62725521736226 19.618891345094863 13.856462100709873 24.897391336833007 2 16.609927668093544 20.385203196426502 37.41860103770108 25.58626809777887 3 19.773836744477794 24.11359046458453 28.770703343108167 27.341869447829513 4 14.051367257992386 16.851309165524707 34.86892527496862 34.22839830151428 5 14.3677146265109 37.56909487330752 33.3963817323992 14.666808767782374 6 33.435674843939964 36.137210827817945 16.19252978740812 14.234584540833975 7 29.01762542437493 31.721420515445487 20.123573960232058 19.13738009994752 8 26.565731813569077 35.77564243340266 18.652996252262493 19.005629500765767 9 25.85852757407559 16.059866195292866 19.178375655064613 38.90323057556693 10-11 25.824291856382036 26.216506344957484 26.399910576531973 21.559291222128504 12-13 26.400935287566657 23.124360699334147 27.196722761552806 23.277981251546386 14-15 23.321816293982163 24.599868556114213 24.70274860540477 27.375566544498852 16-17 23.48691202247278 27.947986487769143 25.200342847648642 23.364758642109436 18-19 23.46527659533239 26.459913112766625 27.264713602805884 22.8100966890951 20-21 23.90172683889422 25.70593374233833 27.387288428907286 23.005050989860163 22-23 23.69518977076076 25.380936531959197 27.400104244509894 23.52376945277015 24-25 23.674525734900303 25.8217069327062 27.06798674467421 23.435780587719286 26-27 23.48682230935559 26.03955523107318 27.098923604077353 23.37469885549388 28-29 23.41388091044671 25.951527913542872 27.283006707633035 23.35158446837738 30-31 23.24325653013762 25.764578091980827 27.270319761527205 23.721845616354347 32-33 23.336035407579015 25.826213923899825 27.092331276455567 23.74541939206559 34-35 23.69774224426321 25.779861863063395 27.004452354957564 23.517943537715833 36-37 23.66122043192972 25.671841136903815 26.969528584882656 23.697409846283804 38-39 23.583343679958432 25.77504594978071 27.148856476640432 23.492753893620428 40-41 23.59224133631079 25.61061995172097 27.152003594193452 23.64513511777479 42-43 23.660123635901705 25.794147336072264 26.750142012271727 23.7955870157543 44-45 23.734361975040883 25.779779575751682 26.66754464123914 23.8183138079683 46-47 23.792856466184194 26.01349460158522 26.400684813531488 23.792964118699096 48-49 23.87744992233775 26.092524023982804 26.105567908670558 23.924458145008884 50-51 23.688064021183393 26.192912207089513 26.070621608872557 24.048402162854533 52-53 24.11344196538867 26.077632106113708 25.888432980897257 23.920492947600362 54-55 23.800118545849557 25.98895859860767 25.920378800523704 24.290544055019073 56-57 23.685718135578107 26.312981061566372 26.07341803085002 23.9278827720055 58-59 23.89152587380091 25.977290730502133 26.0172297633711 24.113953632325856 60-61 23.87684498203641 25.943877953801348 26.231686539292394 23.94759052486985 62-63 23.805251875131425 26.102615315828036 26.15683641590993 23.935296393130606 64-65 23.715244518036734 25.922947342420034 26.038769030156722 24.32303910938651 66-67 23.552552975168158 26.25171605654514 25.9627508027411 24.232980165545605 68-69 23.5500813776297 26.051158368528792 26.184021635630252 24.214738618211257 70-71 23.476986019058835 26.01546675594833 26.24295915597251 24.26458806902032 72-73 23.622047809586117 26.024466764505956 26.018829095685604 24.33465633022232 74-75 23.59710939357374 26.19399001475806 25.88747089880635 24.321429692861855 76-77 23.68519169095765 26.07976318935055 26.03485342335143 24.200191696340365 78-79 23.631008354464853 26.22144328794474 25.950640422849354 24.19690793474105 80-81 23.510372048818972 26.291951056151717 26.012556945927408 24.1851199491019 82-83 23.629162338140016 26.122573109487952 25.956393570276433 24.2918709820956 84-85 23.396858991196908 26.119100112389066 25.848480020439595 24.635560875974434 86-87 23.64157304036055 26.025646063227565 26.043911109867125 24.288869786544762 88-89 23.50293526976151 25.996770745733816 25.93935540151896 24.560938582985717 90-91 23.36827026313221 26.23660798870076 26.06386109053746 24.33126065762957 92-93 23.5700218826233 26.07742673555614 26.066948339353623 24.28560304246693 94-95 23.4697898211709 25.90117012431158 26.140724969649686 24.488315084867835 96-97 23.623206582304736 25.858533988520765 26.041619006966528 24.476640422207975 98-99 23.227055334748602 25.795227986168822 25.999067730559062 24.978648948523514 100-101 23.18980191217735 25.812223229579878 26.236055059023723 24.761919799219044 102-103 23.429358426637485 25.70870516390329 26.178630464960683 24.68330594449854 104-105 23.46886139241835 25.68392152627002 26.178926175965728 24.668290905345902 106-107 23.10458697818873 25.737028561073437 26.310851629004784 24.84753283173305 108-109 23.284721115795186 25.67205574382757 26.07385023690696 24.969372903470283 110-111 23.08057360130676 25.70329387988276 26.31233798296507 24.903794535845417 112-113 23.23482297991995 25.765606194464794 26.196610670653158 24.802960154962093 114-115 23.115871183787682 25.823222823899776 26.129655542639252 24.931250449673286 116-117 22.824256086305613 25.75227469437495 26.318633762363874 25.10483545695556 118-119 23.03690782660369 25.791035788950907 26.023441127688905 25.148615256756496 120-121 22.958935226137058 25.70128772614154 26.239003550801215 25.100773496920187 122-123 22.97099133699939 25.74303311418213 26.117359858027484 25.168615690791 124-125 22.898794389488096 25.73383622429846 26.093689160989108 25.27368022522434 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 106.0 1 96.0 2 143.0 3 205.5 4 180.5 5 173.5 6 326.0 7 677.0 8 1098.0 9 1367.5 10 1240.5 11 966.5 12 948.5 13 1052.5 14 1208.5 15 1405.5 16 1570.5 17 1767.5 18 2079.0 19 2423.0 20 2736.5 21 3203.5 22 3797.0 23 4519.5 24 5626.0 25 7285.5 26 9387.0 27 12109.0 28 14822.5 29 17953.0 30 22025.0 31 26147.5 32 30424.0 33 34460.5 34 39033.5 35 42867.5 36 47005.5 37 52624.0 38 58216.5 39 62378.0 40 65828.5 41 71743.5 42 77826.5 43 83749.0 44 89722.5 45 94060.0 46 98022.0 47 101764.0 48 108552.5 49 114023.0 50 114760.5 51 114886.0 52 114934.0 53 115555.5 54 114567.5 55 109677.5 56 103250.5 57 96008.0 58 89240.5 59 81389.5 60 70483.0 61 57890.5 62 45362.0 63 34931.0 64 26215.0 65 19458.5 66 14499.0 67 11243.0 68 8462.5 69 6135.5 70 4438.0 71 3356.5 72 2589.0 73 1978.0 74 1469.0 75 1075.5 76 765.0 77 469.0 78 270.0 79 162.0 80 111.0 81 76.5 82 44.0 83 34.0 84 25.5 85 15.0 86 8.5 87 6.5 88 4.0 89 2.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10298742476894215 2 3.94725321414064E-4 3 0.0 4 0.17855938175967112 5 0.0 6 0.0 7 0.03179333043389643 8 0.0 9 0.0 10-11 2.6913090096413454E-4 12-13 4.126673814783396E-4 14-15 2.6913090096413454E-4 16-17 0.0031398605112482364 18-19 0.0011662339041779164 20-21 0.0023862939885486594 22-23 0.001094465663920814 24-25 0.003391049352148095 26-27 0.0031398605112482364 28-29 0.0025836566492556915 30-31 5.382618019282691E-4 32-33 0.0 34-35 7.356244626353011E-4 36-37 7.715085827638522E-4 38-39 3.588412012855127E-4 40-41 0.0011124077239850895 42-43 6.100300421853716E-4 44-45 0.0011124077239850895 46-47 1.4353648051420507E-4 48-49 5.38261801928269E-5 50-51 0.04198442055040499 52-53 5.38261801928269E-5 54-55 0.1384588775160151 56-57 2.6913090096413454E-4 58-59 1.7942060064275634E-5 60-61 0.0 62-63 3.588412012855127E-4 64-65 0.05138606002408542 66-67 0.04636228320608825 68-69 0.0017403798262347367 70-71 0.0022068733879059034 72-73 0.06850278532540438 74-75 0.0048622982774186975 76-77 0.0017583218862990125 78-79 0.0012380021444350188 80-81 1.2559442044992946E-4 82-83 1.076523603856538E-4 84-85 0.0 86-87 1.6147854057848072E-4 88-89 0.0013097703846921215 90-91 4.306094415426152E-4 92-93 0.002224815447970179 94-95 0.004341978535554704 96-97 0.0023145257482915568 98-99 0.0 100-101 0.0010585815437922626 102-103 0.0010226974236637112 104-105 2.3324678083558328E-4 106-107 1.7942060064275634E-5 108-109 0.0 110-111 1.4353648051420507E-4 112-113 7.176824025710254E-5 114-115 8.971030032137818E-5 116-117 0.0 118-119 7.89450642828128E-4 120-121 0.0019018583668132174 122-123 4.126673814783396E-4 124-125 0.006925635184810396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2786748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.59696263328406 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1775822038289 38.78934899831601 2 11.59783856356692 11.968264859824465 3 4.8549717964623875 7.5150539510315335 4 2.510179709867971 5.1807059477154205 5 1.5323664515963504 3.953272727175748 6 0.9736808705953787 3.0143385298111944 7 0.6587784329518842 2.3793676332042257 8 0.4855040728837551 2.0040428405512265 9 0.35655571885199855 1.6557472892061276 >10 1.7145966619017867 15.534554024918501 >50 0.09434479004022961 3.3206412360085995 >100 0.04168658922045907 3.716850676030778 >500 0.0015596681890409567 0.5155100331400407 >1k 3.544700429420286E-4 0.4523012530661805 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4071 0.14608425304333222 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3818 0.13700557065080876 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.588412012855127E-5 0.0 0.0 0.0 0.0 6 3.588412012855127E-5 0.0 0.0 0.0 7.176824025710254E-5 7 3.588412012855127E-5 0.0 0.0 0.0 7.176824025710254E-5 8 3.588412012855127E-5 0.0 0.0 0.0 7.176824025710254E-5 9 3.588412012855127E-5 0.0 0.0 0.0 7.176824025710254E-5 10-11 3.588412012855127E-5 0.0 0.0 3.588412012855127E-5 7.176824025710254E-5 12-13 3.588412012855127E-5 0.0 0.0 3.588412012855127E-5 7.176824025710254E-5 14-15 3.588412012855127E-5 3.588412012855127E-5 0.0 3.588412012855127E-5 7.176824025710254E-5 16-17 3.588412012855127E-5 3.588412012855127E-5 0.0 1.0765236038565382E-4 7.176824025710254E-5 18-19 7.176824025710254E-5 3.588412012855127E-5 0.0 1.0765236038565382E-4 7.176824025710254E-5 20-21 7.176824025710254E-5 3.588412012855127E-5 0.0 1.7942060064275636E-4 7.176824025710254E-5 22-23 7.176824025710254E-5 3.588412012855127E-5 0.0 3.4089914122123705E-4 1.2559442044992946E-4 24-25 7.176824025710254E-5 3.588412012855127E-5 0.0 6.279721022496472E-4 1.7942060064275636E-4 26-27 7.176824025710254E-5 3.588412012855127E-5 0.0 9.329871233423331E-4 1.7942060064275636E-4 28-29 7.176824025710254E-5 3.588412012855127E-5 0.0 0.0017044957061061856 1.7942060064275636E-4 30-31 7.176824025710254E-5 3.588412012855127E-5 0.0 0.003749890553433608 1.7942060064275636E-4 32-33 7.176824025710254E-5 3.588412012855127E-5 0.0 0.008648072950980856 2.8707296102841015E-4 34-35 7.176824025710254E-5 5.38261801928269E-5 0.0 0.015340461354955669 3.2295708115696143E-4 36-37 7.176824025710254E-5 7.176824025710254E-5 0.0 0.026590133015256492 3.2295708115696143E-4 38-39 7.176824025710254E-5 1.0765236038565382E-4 0.0 0.04699025530833789 3.2295708115696143E-4 40-41 1.0765236038565382E-4 1.0765236038565382E-4 0.0 0.07072760077337456 3.2295708115696143E-4 42-43 1.0765236038565382E-4 1.0765236038565382E-4 0.0 0.09385491619622585 3.2295708115696143E-4 44-45 1.0765236038565382E-4 1.0765236038565382E-4 0.0 0.12132421015463185 3.2295708115696143E-4 46-47 1.0765236038565382E-4 1.0765236038565382E-4 0.0 0.15557560281733404 3.4089914122123705E-4 48-49 1.0765236038565382E-4 1.0765236038565382E-4 0.0 0.19465340963732639 5.023776817997178E-4 50-51 1.0765236038565382E-4 1.0765236038565382E-4 0.0 0.23600985808548172 5.023776817997178E-4 52-53 1.0765236038565382E-4 1.0765236038565382E-4 0.0 0.27795839451575816 5.562038619925447E-4 54-55 1.0765236038565382E-4 1.7942060064275636E-4 0.0 0.3245718665627463 6.100300421853716E-4 56-57 1.0765236038565382E-4 2.511888408998589E-4 0.0 0.3802281368821293 6.279721022496472E-4 58-59 1.0765236038565382E-4 2.511888408998589E-4 0.0 0.4437968556898578 6.997403425067499E-4 60-61 1.0765236038565382E-4 2.511888408998589E-4 0.0 0.5149909500249036 7.176824025710254E-4 62-63 1.2559442044992946E-4 3.050150210926858E-4 0.0 0.5862029864200136 7.535665226995767E-4 64-65 1.4353648051420507E-4 3.767832613497884E-4 0.0 0.6605189992062432 7.89450642828128E-4 66-67 1.4353648051420507E-4 4.6649356167116656E-4 0.0 0.7523285205551417 7.89450642828128E-4 68-69 1.4353648051420507E-4 4.6649356167116656E-4 0.0 0.8544906105611272 7.89450642828128E-4 70-71 1.4353648051420507E-4 5.023776817997178E-4 0.0 0.9746127026914525 8.253347629566792E-4 72-73 1.4353648051420507E-4 5.741459220568203E-4 0.0 1.1000994707809963 9.688712434708844E-4 74-75 1.4353648051420507E-4 5.741459220568203E-4 0.0 1.2286722732015956 9.688712434708844E-4 76-77 1.4353648051420507E-4 6.459141623139229E-4 0.0 1.3705042580096944 9.688712434708844E-4 78-79 1.4353648051420507E-4 6.459141623139229E-4 0.0 1.532287813609268 9.688712434708844E-4 80-81 1.6147854057848072E-4 7.176824025710254E-4 0.0 1.7139332296999945 0.0010047553635994357 82-83 1.7942060064275636E-4 7.715085827638523E-4 0.0 1.9082457401960995 0.0010765236038565381 84-85 1.7942060064275636E-4 7.89450642828128E-4 0.0 2.1087482614143798 0.0010765236038565381 86-87 1.7942060064275636E-4 8.253347629566792E-4 0.0 2.321612862016946 0.0010765236038565381 88-89 1.97362660707032E-4 8.432768230209549E-4 0.0 2.555972050576514 0.0011124077239850895 90-91 2.1530472077130764E-4 8.612188830852306E-4 0.0 2.829660234796975 0.0011124077239850895 92-93 2.1530472077130764E-4 8.612188830852306E-4 0.0 3.1309433073962913 0.001184175964242192 94-95 2.1530472077130764E-4 8.612188830852306E-4 0.0 3.434935631065313 0.0012559442044992946 96-97 2.1530472077130764E-4 8.612188830852306E-4 0.0 3.7459433002194675 0.0012918283246278457 98-99 2.1530472077130764E-4 8.791609431495061E-4 0.0 4.0848149886534415 0.0012918283246278457 100-101 2.1530472077130764E-4 0.0010226974236637112 0.0 4.469600408791896 0.001327712444756397 102-103 2.1530472077130764E-4 0.0011662339041779162 0.0 4.888691047773246 0.0013635965648849484 104-105 2.1530472077130764E-4 0.0012021180243064677 0.0 5.31291311593298 0.0013635965648849484 106-107 2.511888408998589E-4 0.0012559442044992946 0.0 5.741674525288975 0.0013635965648849484 108-109 2.511888408998589E-4 0.0012918283246278457 0.0 6.192791741485057 0.0013994806850134997 110-111 2.511888408998589E-4 0.0012918283246278457 0.0 6.686054856772123 0.0015609592255919802 112-113 2.511888408998589E-4 0.0013097703846921215 0.0 7.224119296039685 0.0016147854057848073 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3990 0.0 61.030743 1 GTATCAA 7115 0.0 50.58291 1 TCAACGC 8825 0.0 39.042988 4 ATCAACG 9005 0.0 38.72595 3 CAACGCA 9000 0.0 38.206173 5 TATCAAC 9495 0.0 37.6064 2 AACGCAG 9195 0.0 37.007732 6 GTACATG 8925 0.0 30.694872 1 ACGCAGA 10970 0.0 30.640087 7 CGCAGAG 11245 0.0 29.827166 8 TACATGG 9405 0.0 28.26871 2 GCAGAGT 12230 0.0 26.355127 9 ACATGGG 9375 0.0 25.179888 3 GAGTACT 7335 0.0 23.879019 12-13 GTACTTT 7795 0.0 22.813206 14-15 CAGAGTA 12010 0.0 22.705318 10-11 GTGGTAT 2235 0.0 22.698795 1 CATGGGG 5500 0.0 22.652363 4 AGAGTAC 11545 0.0 22.615274 10-11 AGTACTT 7615 0.0 22.610487 12-13 >>END_MODULE