FastQCFastQC Report
Thu 26 May 2016
SRR1364479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2651836
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.053.4260061
GTATCAA62250.052.2068861
ATCAACG69950.044.316013
CAACGCA72850.042.7571755
TCAACGC72850.042.103944
AACGCAG72400.042.015166
TATCAAC81100.040.056612
GTACATG109150.034.4096371
ACGCAGA87650.034.027827
CGCAGAG86650.033.323158
TACATGG111450.032.5116962
ACATGGG114300.029.5153163
GCAGAGT96550.027.995279
CATGGGA68400.023.9453774
GAGTACA87800.022.5070251
CAGAGTA98450.022.15393310-11
AGAGTAC91150.022.10011710-11
GAGTACT43050.022.03857412-13
CATGGGG54900.021.805644
AGTACAT83950.021.4036922
Produced by FastQC (version 0.11.5)