##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364479_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2651836 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.380299158771507 30.0 26.0 31.0 10.0 34.0 2 26.62813311230408 31.0 26.0 31.0 10.0 34.0 3 26.607693311351078 31.0 26.0 31.0 10.0 34.0 4 29.42323771153269 35.0 28.0 37.0 10.0 37.0 5 30.02329480405274 35.0 30.0 37.0 10.0 37.0 6 30.296613742327956 35.0 30.0 37.0 10.0 37.0 7 30.136770901367957 35.0 30.0 37.0 10.0 37.0 8 29.79100856915737 35.0 30.0 37.0 8.0 37.0 9 30.449903010593417 35.0 28.0 39.0 8.0 39.0 10-11 30.559399412331683 35.0 28.5 39.0 7.5 39.0 12-13 30.38745156186129 35.0 28.0 39.0 4.5 39.0 14-15 31.064911442487393 36.0 27.5 40.0 2.0 41.0 16-17 31.308257373382062 36.5 29.0 40.0 2.0 41.0 18-19 31.23342770819915 37.0 29.0 40.0 2.0 41.0 20-21 31.00342423136272 36.5 27.5 40.0 2.0 41.0 22-23 31.064464205177092 37.0 28.0 40.0 2.0 41.0 24-25 31.09397187458048 37.0 28.5 40.0 2.0 41.0 26-27 31.20688345734804 37.0 29.0 40.0 2.0 41.0 28-29 31.15545380634398 37.0 29.0 40.0 2.0 41.0 30-31 30.802430467042456 36.0 28.0 40.0 2.0 41.0 32-33 30.841956101357702 36.0 29.0 40.0 2.0 41.0 34-35 30.548707009030725 36.0 28.0 40.0 2.0 41.0 36-37 30.31630048012019 36.0 27.0 40.0 2.0 41.0 38-39 30.183263218389072 36.0 27.0 40.0 2.0 41.0 40-41 30.249933065242345 36.0 27.5 40.0 2.0 41.0 42-43 30.130371749987557 36.0 27.0 40.0 2.0 41.0 44-45 29.791327781959367 35.0 26.5 40.0 2.0 41.0 46-47 29.555096921529085 35.0 26.5 40.0 2.0 41.0 48-49 29.33753293944271 35.0 26.0 40.0 2.0 41.0 50-51 29.342267772215173 35.0 26.0 40.0 2.0 41.0 52-53 29.163784261168487 35.0 26.0 39.5 2.0 41.0 54-55 29.037720469893312 35.0 25.5 39.0 2.0 41.0 56-57 28.642099473723114 34.0 24.0 39.0 2.0 41.0 58-59 28.39131322600643 34.0 23.5 39.0 2.0 40.0 60-61 27.982756663685088 34.0 22.5 38.5 2.0 40.0 62-63 27.92324148250495 34.0 23.0 38.0 2.0 40.0 64-65 27.728201517740917 33.5 22.5 38.0 2.0 40.0 66-67 27.502739234251287 33.0 22.5 38.0 2.0 40.0 68-69 27.320595428978265 33.0 22.5 37.0 2.0 40.0 70-71 27.185799008686814 33.0 22.5 37.0 2.0 39.5 72-73 27.16108311373705 33.0 22.5 36.5 2.0 39.0 74-75 26.871366102579493 33.0 22.0 36.0 2.0 39.0 76-77 26.57509778131076 33.0 22.0 36.0 2.0 39.0 78-79 26.276778616777207 33.0 21.0 35.0 2.0 37.5 80-81 26.269825132474253 33.0 22.0 35.0 2.0 37.0 82-83 26.068076985152928 33.0 22.0 35.0 2.0 37.0 84-85 25.871738486090393 33.0 21.0 35.0 2.0 36.0 86-87 25.663703562362077 33.0 20.0 35.0 2.0 36.0 88-89 25.432802028481397 32.0 20.0 35.0 2.0 36.0 90-91 25.269267028579446 32.0 19.0 35.0 2.0 35.0 92-93 25.15972669501432 32.0 18.5 35.0 2.0 35.0 94-95 25.101856034837752 32.0 18.0 35.0 2.0 35.0 96-97 24.97997877696811 32.0 18.0 35.0 2.0 35.0 98-99 24.88561924643907 32.0 17.0 35.0 2.0 35.0 100-101 24.818393181177118 32.0 16.0 35.0 2.0 35.0 102-103 23.67349658877849 30.5 10.0 34.0 2.0 35.0 104-105 24.47760853235268 32.0 10.0 34.5 2.0 35.0 106-107 24.54558275851146 32.0 7.5 35.0 2.0 35.0 108-109 24.513297390939712 32.0 7.0 35.0 2.0 35.0 110-111 24.40785591567503 32.0 6.0 35.0 2.0 35.0 112-113 24.39119293199127 32.0 2.0 35.0 2.0 35.0 114-115 24.34645958498188 32.0 2.0 35.0 2.0 35.0 116-117 24.16307135886231 32.0 2.0 35.0 2.0 35.0 118-119 24.011054605186743 32.0 2.0 35.0 2.0 35.0 120-121 23.848254944875926 32.0 2.0 35.0 2.0 35.0 122-123 23.58966617845146 32.0 2.0 35.0 2.0 35.0 124-125 22.570441573309964 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.3881790552599647 1101 2 1.9964023991226298 1101 3 2.337619567398921 1101 4 2.7685384073579122 1101 5 2.235703267460611 1101 6 2.1286160094536193 1101 7 1.9724589899481693 1101 8 2.4377896979502154 1101 9 3.0026354893153524 1101 10-11 3.030165644822258 1101 12-13 2.842951721778917 1101 14-15 3.285249219238896 1101 16-17 2.938291871453689 1101 18-19 2.8645438282402367 1101 20-21 3.21833297485756 1101 22-23 3.0938800211561457 1101 24-25 2.9556893179800667 1101 26-27 2.656358705864971 1101 28-29 2.5402736516485582 1101 30-31 2.72807692387099 1101 32-33 2.8751518115523247 1101 34-35 3.1701568200550554 1101 36-37 3.2535086489188814 1101 38-39 3.2898721698416296 1101 40-41 3.197690888772332 1101 42-43 3.061705072001338 1101 44-45 3.281956235834908 1101 46-47 3.298913360403823 1101 48-49 3.3170563803822795 1101 50-51 3.2641088054360026 1101 52-53 3.233280553096275 1101 54-55 3.2889703659399387 1101 56-57 3.3665392455785756 1101 58-59 3.3957227659982614 1101 60-61 3.6165177131137725 1101 62-63 3.199524030533535 1101 64-65 3.1679002685961137 1101 66-67 3.2476020847266938 1101 68-69 3.1769283411088907 1101 70-71 3.221554608592644 1101 72-73 2.982076277436235 1101 74-75 3.06250914583876 1101 76-77 3.255454285467465 1101 78-79 3.13241070554918 1101 80-81 2.960772902590172 1101 82-83 3.00214094694142 1101 84-85 2.809821755058657 1101 86-87 2.851659607416181 1101 88-89 3.0280720903651215 1101 90-91 3.0102507387138573 1101 92-93 3.000812435925326 1101 94-95 2.9504690090401127 1101 96-97 3.017676272640493 1101 98-99 2.9134471467627847 1101 100-101 2.9395513646286204 1101 102-103 2.9237400459890246 1101 104-105 2.9449564270203297 1101 106-107 3.052780176039466 1101 108-109 3.084006164077426 1101 110-111 3.195202409033598 1101 112-113 3.198999776173956 1101 114-115 3.2060413416345206 1101 116-117 3.2738141713608684 1101 118-119 3.2979031207619194 1101 120-121 3.2758762589221 1101 122-123 3.373566849937692 1101 124-125 3.4026943175447038 1102 1 1.2291644156722938 1102 2 1.049700413555211 1102 3 1.187238473998537 1102 4 1.7138600022989898 1102 5 1.6193889415501346 1102 6 1.5359220115131542 1102 7 1.6625421757165313 1102 8 1.630204748654112 1102 9 2.0639117428044997 1102 10-11 1.7317362986658331 1102 12-13 2.0952584424347975 1102 14-15 2.4299341509986156 1102 16-17 1.927031268861203 1102 18-19 2.171175167215985 1102 20-21 2.1616917337559585 1102 22-23 2.082843356919966 1102 24-25 1.820217898996205 1102 26-27 2.016502346023561 1102 28-29 1.8001543514691143 1102 30-31 1.6984436717148732 1102 32-33 1.6459981862007744 1102 34-35 2.051136062509464 1102 36-37 2.2722457389069177 1102 38-39 2.1788313654356415 1102 40-41 1.7841570165137135 1102 42-43 1.8179389942320725 1102 44-45 2.2074546714045056 1102 46-47 2.153770504767852 1102 48-49 2.2695315294745413 1102 50-51 2.2003595256805113 1102 52-53 2.2759283630914844 1102 54-55 1.956265345127015 1102 56-57 2.1885580662103337 1102 58-59 2.3005483844196455 1102 60-61 2.67181002360541 1102 62-63 2.4246934940123595 1102 64-65 2.493970812342809 1102 66-67 2.4914450061570825 1102 68-69 2.2930281165640167 1102 70-71 2.5125329940055323 1102 72-73 2.4441645579867988 1102 74-75 2.475482407461893 1102 76-77 2.3457429502363354 1102 78-79 2.3431952413557156 1102 80-81 1.9724088893679976 1102 82-83 1.8329897476766313 1102 84-85 1.9402793493035553 1102 86-87 2.0513629919390652 1102 88-89 1.8171580753629364 1102 90-91 1.796734376472731 1102 92-93 1.7745169781135246 1102 94-95 1.9304939027999275 1102 96-97 2.0389115463597776 1102 98-99 2.193063772313433 1102 100-101 2.0434115851124375 1102 102-103 1.8126850542495845 1102 104-105 1.7979706947838352 1102 106-107 2.1073934986839546 1102 108-109 2.2291542674445424 1102 110-111 2.0042309590643796 1102 112-113 1.987334307167874 1102 114-115 2.035967701990156 1102 116-117 2.1742702229310353 1102 118-119 2.405909813596921 1102 120-121 2.2222910960248754 1102 122-123 2.410033149087372 1102 124-125 2.314166174279009 1103 1 1.779587234170986 1103 2 1.5276752341774653 1103 3 1.6518385763197116 1103 4 2.2649675156667612 1103 5 1.61541349503889 1103 6 1.6949051590584787 1103 7 1.75981750859453 1103 8 1.9790588186153535 1103 9 2.826389724282393 1103 10-11 2.3152745940001545 1103 12-13 2.4921026273020956 1103 14-15 2.7946065797960777 1103 16-17 2.6271063057971595 1103 18-19 2.3155078311767383 1103 20-21 2.7300247730598954 1103 22-23 2.58479745039827 1103 24-25 2.6077888986417364 1103 26-27 2.366879583361392 1103 28-29 2.107349026245384 1103 30-31 2.345469597393649 1103 32-33 2.4106178799146 1103 34-35 2.35583655962256 1103 36-37 2.699739093657641 1103 38-39 2.712517940877646 1103 40-41 2.6992615628527723 1103 42-43 2.7239935112447995 1103 44-45 2.6228018910620072 1103 46-47 2.8077298708034917 1103 48-49 2.8878586010120237 1103 50-51 2.6573992419455763 1103 52-53 2.67992813505359 1103 54-55 2.7270885835831677 1103 56-57 2.9060814626815272 1103 58-59 3.0488381134312696 1103 60-61 2.9285700447374836 1103 62-63 2.70565427429505 1103 64-65 2.629299255330615 1103 66-67 2.6219272476533355 1103 68-69 2.5819435676053715 1103 70-71 2.7224265521939515 1103 72-73 2.6691690608898178 1103 74-75 2.6930727200571454 1103 76-77 2.6749445761528534 1103 78-79 2.7685761547214476 1103 80-81 2.294079347243887 1103 82-83 2.466144433715314 1103 84-85 2.460324123138413 1103 86-87 2.4365624767509537 1103 88-89 2.4885449309227567 1103 90-91 2.518082823286882 1103 92-93 2.414514306752107 1103 94-95 2.2569508316673748 1103 96-97 2.4181808043209863 1103 98-99 2.4147751723958244 1103 100-101 2.2181927234199037 1103 102-103 2.2233505228191106 1103 104-105 2.396002446682836 1103 106-107 2.2821825094765984 1103 108-109 2.5229382957994417 1103 110-111 2.6625792620075472 1103 112-113 2.572225454737925 1103 114-115 2.5300628625833035 1103 116-117 2.5816880271850167 1103 118-119 2.733615614493104 1103 120-121 2.709600015883453 1103 122-123 2.687662996352177 1103 124-125 2.6832326914424236 1104 1 1.271148137441216 1104 2 1.257231197992791 1104 3 1.23043702781575 1104 4 1.562552715644646 1104 5 1.3145679196821014 1104 6 1.1932164466651791 1104 7 1.2705791304768788 1104 8 1.614701371699244 1104 9 2.0670469689985396 1104 10-11 1.9734243233215203 1104 12-13 1.4752310313552464 1104 14-15 1.9032285806495928 1104 16-17 1.5878924714301554 1104 18-19 0.9136090068183762 1104 20-21 -3.7279928203741832 1104 22-23 -0.38691429659599663 1104 24-25 1.0875969476473557 1104 26-27 1.2843466562542254 1104 28-29 0.8856382325039043 1104 30-31 1.5698549926779712 1104 32-33 1.3806440251299215 1104 34-35 1.715767200949962 1104 36-37 1.8816335972723266 1104 38-39 1.802715697683194 1104 40-41 1.8903374210035402 1104 42-43 1.844699019651138 1104 44-45 2.0273128036490284 1104 46-47 2.0202402198676026 1104 48-49 1.9259637020482927 1104 50-51 1.9415451831407182 1104 52-53 1.9737632887485468 1104 54-55 1.861484457624158 1104 56-57 2.075477392412548 1104 58-59 2.077831723326316 1104 60-61 2.143654953973268 1104 62-63 1.8056000442458533 1104 64-65 1.8981473707548773 1104 66-67 1.8408320414813097 1104 68-69 1.7857119364102445 1104 70-71 1.9651851172454755 1104 72-73 1.8837085339336852 1104 74-75 1.9853284342306168 1104 76-77 1.9087969756958145 1104 78-79 1.8708341647289934 1104 80-81 1.6163348296264246 1104 82-83 1.6546971571735725 1104 84-85 1.613044049342605 1104 86-87 1.5293796467802458 1104 88-89 1.6072859609394818 1104 90-91 1.6475439083474335 1104 92-93 1.3467358262430658 1104 94-95 1.5248668665532144 1104 96-97 1.558946965693437 1104 98-99 1.4361981490189564 1104 100-101 1.027768037263332 1104 102-103 1.4734845551777305 1104 104-105 1.6055260540883864 1104 106-107 1.7181242735581748 1104 108-109 1.7891195611311304 1104 110-111 1.809684178112768 1104 112-113 1.72302455149579 1104 114-115 1.6957811932430147 1104 116-117 1.7286282312433272 1104 118-119 1.6852507289943155 1104 120-121 1.6910704624884048 1104 122-123 1.4938850177558116 1104 124-125 1.6707395862194723 1105 1 1.522284107592963 1105 2 1.2346719788827976 1105 3 1.2876505249479386 1105 4 1.7916093047201613 1105 5 1.2515154074964983 1105 6 1.384487167082952 1105 7 1.2953181153084508 1105 8 1.5068036822223583 1105 9 1.9245560773562858 1105 10-11 1.7917373123107332 1105 12-13 1.8252029303433233 1105 14-15 2.053281152789463 1105 16-17 1.8737386463277552 1105 18-19 1.467493665827483 1105 20-21 1.7538469006712774 1105 22-23 1.6606971377832451 1105 24-25 1.7244244555607011 1105 26-27 1.6092091387114884 1105 28-29 1.181920323839126 1105 30-31 1.639702627278357 1105 32-33 1.6017563174261262 1105 34-35 1.4622854692587453 1105 36-37 1.5803142771944572 1105 38-39 1.49180955832583 1105 40-41 1.5633295343668543 1105 42-43 1.8268027229892851 1105 44-45 2.0727048086868187 1105 46-47 2.279486176727449 1105 48-49 1.8674888202336426 1105 50-51 2.0593329299575345 1105 52-53 1.9821376146199512 1105 54-55 2.1116600889486676 1105 56-57 2.418295717942989 1105 58-59 2.1624520170695796 1105 60-61 2.474103685124895 1105 62-63 2.3035623640108973 1105 64-65 2.0167251271521387 1105 66-67 2.182094513089705 1105 68-69 1.718942055963943 1105 70-71 1.8638914714593469 1105 72-73 1.8052033711374662 1105 74-75 2.0265665672630107 1105 76-77 1.9688211857983902 1105 78-79 1.7995607810367993 1105 80-81 1.6880821374964583 1105 82-83 1.8230315991849544 1105 84-85 1.7389400494959553 1105 86-87 1.67303495626048 1105 88-89 1.8682717640146045 1105 90-91 1.9204500056960256 1105 92-93 1.9114270373151072 1105 94-95 1.7704556398522762 1105 96-97 1.9250280042807333 1105 98-99 1.7904276673986104 1105 100-101 1.9120998469970516 1105 102-103 1.9408419568170494 1105 104-105 2.0211615855951663 1105 106-107 2.058011569739172 1105 108-109 2.0595969500128355 1105 110-111 1.9677945318877903 1105 112-113 2.04888402849069 1105 114-115 1.9342438721218613 1105 116-117 2.0810260856964007 1105 118-119 2.128470370826733 1105 120-121 2.1367747395113597 1105 122-123 2.1707498400607577 1105 124-125 1.8448868897662756 1106 1 0.3018914711235716 1106 2 0.3837858296373824 1106 3 0.3718002541190337 1106 4 0.08198338681802042 1106 5 -0.34037480339717874 1106 6 0.1374702116123956 1106 7 0.2541898692096325 1106 8 0.5924039402244539 1106 9 0.8205964153139007 1106 10-11 -1.9208810215443997 1106 12-13 0.05429873979872468 1106 14-15 0.3991132252562153 1106 16-17 0.2732006794973536 1106 18-19 -2.6100733712634607 1106 20-21 -5.213095559835246 1106 22-23 -6.097038841467686 1106 24-25 -5.8772868763227635 1106 26-27 -4.669574010736017 1106 28-29 -1.1692389840786284 1106 30-31 -3.1050329886335923 1106 32-33 -3.705731371455176 1106 34-35 -2.9432758161864356 1106 36-37 -5.657783596068295 1106 38-39 -5.936577033313604 1106 40-41 -2.042726080581769 1106 42-43 -0.6644882665178642 1106 44-45 0.1957214961032463 1106 46-47 0.3410547802596895 1106 48-49 0.5260194857303659 1106 50-51 0.3349284080100787 1106 52-53 0.24221948630788503 1106 54-55 0.2798975859513817 1106 56-57 0.4094207442510367 1106 58-59 0.6558382265224658 1106 60-61 0.8551514731024561 1106 62-63 0.6677891011534598 1106 64-65 0.42788316809531324 1106 66-67 0.11985080058194342 1106 68-69 0.25136424958907 1106 70-71 0.5253045041253017 1106 72-73 0.5196669068760968 1106 74-75 0.39983863905963446 1106 76-77 0.4245338799413041 1106 78-79 0.3574865970574308 1106 80-81 0.03411540762067489 1106 82-83 -0.14168917951228366 1106 84-85 -0.20656978212207378 1106 86-87 -0.20211066728472105 1106 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-7.96004145587786 2216 116-117 -7.473840876335984 2216 118-119 -7.679528726766279 2216 120-121 -8.532574647581537 2216 122-123 -11.150641309912633 2216 124-125 -9.900644264198734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 229580.0 3 34300.0 4 20127.0 5 16598.0 6 16729.0 7 18945.0 8 20680.0 9 21929.0 10 23419.0 11 24252.0 12 24480.0 13 24356.0 14 24607.0 15 21321.0 16 18489.0 17 16388.0 18 15129.0 19 14502.0 20 14658.0 21 16276.0 22 19932.0 23 26739.0 24 36257.0 25 46195.0 26 55641.0 27 64298.0 28 74067.0 29 83837.0 30 94179.0 31 106651.0 32 126541.0 33 153234.0 34 189755.0 35 237385.0 36 307737.0 37 320206.0 38 92181.0 39 236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50980084276831 19.100772864732818 13.905488260578766 23.48393803192011 2 15.04036182299643 20.17242419898756 40.68196392332008 24.10525005469593 3 19.01607729414654 25.312090093509138 29.05634126402382 26.615491348320496 4 11.310299242471402 16.62884494149045 38.32549923734048 33.735356578697676 5 13.697633171590814 38.25917965190837 34.0418764848563 14.00131069164451 6 31.695651632848467 36.76730735653865 17.758657685016498 13.778383325596383 7 28.573701964141268 32.53637630359813 20.808282691535357 18.081639040725246 8 25.759240725335374 35.57478446666671 19.941254863688506 18.724719944309403 9 25.960187380125067 16.601400900999426 20.52296583125984 36.915445887615675 10-11 24.370070808319525 26.967092693704213 27.764629493808044 20.898207004168214 12-13 25.29495294875641 24.199482112947496 28.092044590403308 22.41352034789279 14-15 22.504856426642974 25.579763625619 25.69620095631347 26.219178991424556 16-17 22.46761987430573 29.25647578941731 25.85031832780268 22.425586008474284 18-19 22.36253732567601 27.899400054874757 27.878657020527122 21.859405598922116 20-21 23.124442585031034 27.04853069001271 27.881113251618157 21.945913473338102 22-23 22.88949926816087 27.013406176563876 27.582886768778437 22.514207786496822 24-25 22.581544208534435 27.171025276701105 27.679425544091625 22.568004970672842 26-27 22.509686187876973 27.51786203187825 27.351215100874377 22.621236679370398 28-29 22.45058884598857 27.419959125175424 27.56542885097395 22.56402317786205 30-31 22.528916295094035 27.135159922045165 27.71488110681381 22.62104267604699 32-33 22.412335689225166 27.262978884840994 27.497807555509716 22.826877870424127 34-35 22.74049586153443 27.17790404492814 27.49517697621396 22.58642311732347 36-37 22.84870972832843 26.89317182963259 27.446383786930056 22.81173465510892 38-39 22.58646132240088 27.13045367139361 27.565197420349463 22.717887585856044 40-41 22.780564838598277 27.055584401671723 27.570306828582964 22.593543931147035 42-43 22.631212233495408 27.105048042246626 27.349431328013846 22.914308396244117 44-45 22.65425063432302 27.1635218867396 27.411550587095633 22.77067689184174 46-47 22.68247499073731 27.31693106578914 27.11363269969935 22.886961243774202 48-49 22.73737011283239 27.326975971616445 27.024299532767287 22.911354382783873 50-51 22.73003857774295 27.348827158550016 26.92978752731545 22.991346736391584 52-53 23.054742273114943 27.274875176047075 26.832827073263683 22.837555477574302 54-55 22.79689281899969 27.180555304141095 26.963370924823053 23.059180952036154 56-57 22.621689414933325 27.46556459804419 26.952957878042742 22.959788108979744 58-59 22.78338058244445 27.128670825996426 26.9329738956361 23.154974695923023 60-61 22.86363453762619 27.069572067743568 27.194539746077595 22.87225364855264 62-63 22.81078441262625 27.235497261659745 27.097679882414944 22.856038443299056 64-65 22.695632695051938 27.1228990867759 26.97833166899473 23.203136549177433 66-67 22.54590043596717 27.46860917592004 26.80056364043994 23.18492674767285 68-69 22.61077421129712 27.27174505974726 27.03746240871226 23.080018320243358 70-71 22.442187035980787 27.083232378465265 27.31981088049929 23.154769705054655 72-73 22.6858783670017 27.113570804068072 26.99584040630186 23.204710422628374 74-75 22.581741794898242 27.29718054891177 26.96453988699419 23.1565377691958 76-77 22.673710739829442 27.053189377096192 27.118016069401897 23.155083813672473 78-79 22.624609916291828 27.160933621085682 27.10582970059826 23.108626762024233 80-81 22.539893479117143 27.38171579435025 26.984492117654536 23.09389860887807 82-83 22.63005562364476 27.335099462619024 27.023984161402847 23.010860752333365 84-85 22.725775453057437 27.192722949058716 26.861757640173796 23.21974395771005 86-87 22.63646296957978 27.026092924638146 27.164434354757056 23.173009751025024 88-89 22.6390557090196 26.99382477443928 27.082427476454008 23.284692040087112 90-91 22.5564225922291 27.235893998402943 27.187642888995107 23.02004052037285 92-93 22.669385584831378 27.056893645424413 27.04705123708891 23.2266695326553 94-95 22.56323135113528 26.89058628764938 27.058568427880246 23.487613933335094 96-97 22.634357297994327 26.86144753347773 27.091555341361463 23.412639827166483 98-99 22.37453882505247 26.81937252489972 27.118699035536984 23.68738961451082 100-101 22.27901196531625 26.92772182709072 27.22433285145748 23.568933356135545 102-103 22.48818830531925 26.7123799079953 27.251217182456884 23.548214604228566 104-105 22.423621679339742 26.72344174159673 27.340128718746353 23.512807860317174 106-107 22.233828548457744 26.775631214975583 27.427751719061373 23.562788517505304 108-109 22.294757721627345 26.80340371935342 27.068788994665898 23.833049564353338 110-111 22.11618832309333 26.779785973344243 27.457653324333066 23.646372379229362 112-113 22.205790570289153 26.86898151203462 27.393621133413156 23.531606784263072 114-115 22.10680453184185 26.94783615242577 27.230402189933926 23.714957125798453 116-117 21.82930469360899 26.831413716815412 27.496892089852498 23.8423894997231 118-119 22.01168363160245 26.93408564325968 27.11903939445135 23.93519133068652 120-121 21.92356297802837 26.966240227300244 27.37212004463641 23.738076750034978 122-123 21.92998251891647 26.8271371430694 27.41999684356231 23.822883494451816 124-125 21.975615328208686 26.948082634490827 27.14754350720242 23.928758530098065 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 301.0 1 318.0 2 423.0 3 488.5 4 463.5 5 446.5 6 440.5 7 498.0 8 606.5 9 696.5 10 739.5 11 774.0 12 848.5 13 947.0 14 1064.0 15 1199.5 16 1333.5 17 1504.5 18 1763.5 19 2051.5 20 2444.5 21 2952.5 22 3790.5 23 5043.5 24 6592.0 25 8650.5 26 11722.5 27 15976.5 28 20295.5 29 24893.0 30 30311.0 31 36213.0 32 42338.5 33 48287.5 34 54493.0 35 59521.0 36 64909.5 37 70752.5 38 74107.5 39 76098.0 40 78009.5 41 81620.0 42 85645.5 43 89663.0 44 94492.0 45 97630.0 46 97829.0 47 97892.0 48 100249.0 49 101027.5 50 99034.0 51 96230.0 52 93858.5 53 92220.5 54 88959.5 55 83418.5 56 76707.0 57 70238.0 58 65307.5 59 59294.0 60 50532.0 61 41273.0 62 32964.0 63 25309.5 64 19067.0 65 14500.0 66 10750.0 67 8216.5 68 6162.5 69 4420.5 70 3230.5 71 2435.0 72 1888.0 73 1476.0 74 1171.5 75 918.0 76 628.5 77 399.5 78 285.5 79 190.5 80 132.0 81 91.0 82 67.0 83 55.0 84 34.0 85 25.0 86 25.0 87 19.5 88 15.0 89 12.5 90 11.5 91 13.0 92 10.0 93 5.5 94 4.5 95 3.0 96 3.5 97 4.0 98 3.5 99 3.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0932184343224845 2 0.03077113365984925 3 0.040688790709531054 4 0.0468731852195988 5 0.0500408019198774 6 0.0022248736347194923 7 0.0015460986275169354 8 0.0015838083501392998 9 0.004298908378949528 10-11 0.04902263940907356 12-13 0.003752117400925246 14-15 0.007353395911361034 16-17 0.015178163355501623 18-19 0.01295328972078213 20-21 0.13009854304715676 22-23 0.011199787618842192 24-25 0.010690706363440274 26-27 0.004600586159928442 28-29 0.0030733423937226887 30-31 0.0016592277953840283 32-33 0.004355472962883074 34-35 0.01851547380758086 36-37 0.0018666312698070319 38-39 0.00599584589695592 40-41 0.029998084346090782 42-43 0.009936511910992988 44-45 0.11120597201335225 46-47 0.002583115999631953 48-49 0.006014700758267103 50-51 0.00669347576546966 52-53 0.007598509108406403 54-55 0.006486072291046655 56-57 0.003601278510435789 58-59 0.010822690392618548 60-61 0.028301146828084392 62-63 0.005222796583197452 64-65 0.004977683386152084 66-67 0.0054867646415540025 68-69 0.004694860466484353 70-71 0.002771664612743774 72-73 5.467909780242821E-4 74-75 9.615979268702891E-4 76-77 0.0056753132546658236 78-79 0.018176086303979582 80-81 0.0020928896055412176 82-83 0.0032430361455233277 84-85 0.001791211824562303 86-87 0.0023945673865201317 88-89 0.004185779211082435 90-91 0.0031487618389674176 92-93 0.0017346472406287567 94-95 0.002488841693076042 96-97 0.001338695153093932 98-99 0.0018666312698070319 100-101 0.001753502101939939 102-103 5.656458393354642E-4 104-105 0.0032241812842121456 106-107 9.615979268702891E-4 108-109 0.0011690014012932927 110-111 0.0023945673865201317 112-113 0.004185779211082435 114-115 0.0025076965543872243 116-117 0.005071957692707995 118-119 0.0034692944812575134 120-121 0.007127137575626849 122-123 0.004242343795015981 124-125 0.05435856516013811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2651836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.67852288208986 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5142363009478 46.856136476559236 2 11.310549041455943 13.499937195590558 3 4.169440798737664 7.464782043389541 4 2.031259890970859 4.848903595311029 5 1.1014178107205796 3.286549400991471 6 0.690236944784574 2.4715392782033994 7 0.46860059892751366 1.9575774095959644 8 0.32476970991989695 1.5505421251891405 9 0.222157527751583 1.193222979301622 >10 1.0755291988323061 11.132990640768407 >50 0.06326844915537319 2.5753433963391568 >100 0.028005592283503924 2.8979183314133974 >500 3.9610163433598553E-4 0.1417931647555283 >1k 1.320338781081834E-4 0.12276396259157064 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.770972262236428E-5 0.0 0.0 0.0 0.0 3 3.770972262236428E-5 0.0 0.0 0.0 0.0 4 3.770972262236428E-5 0.0 0.0 0.0 0.0 5 7.541944524472856E-5 3.770972262236428E-5 0.0 0.0 0.0 6 7.541944524472856E-5 3.770972262236428E-5 0.0 0.0 0.0 7 7.541944524472856E-5 3.770972262236428E-5 0.0 0.0 0.0 8 7.541944524472856E-5 3.770972262236428E-5 0.0 0.0 0.0 9 7.541944524472856E-5 3.770972262236428E-5 0.0 3.770972262236428E-5 0.0 10-11 7.541944524472856E-5 3.770972262236428E-5 0.0 1.1312916786709283E-4 0.0 12-13 7.541944524472856E-5 3.770972262236428E-5 0.0 1.508388904894571E-4 0.0 14-15 1.508388904894571E-4 3.770972262236428E-5 0.0 1.508388904894571E-4 0.0 16-17 1.508388904894571E-4 3.770972262236428E-5 0.0 2.0740347442300353E-4 0.0 18-19 1.8854861311182137E-4 3.770972262236428E-5 0.0 2.6396805835654996E-4 0.0 20-21 2.2625833573418567E-4 3.770972262236428E-5 0.0 3.3938750360127854E-4 0.0 22-23 2.2625833573418567E-4 3.770972262236428E-5 0.0 4.3366181015718917E-4 0.0 24-25 2.6396805835654996E-4 3.770972262236428E-5 0.0 5.467909780242821E-4 0.0 26-27 2.6396805835654996E-4 3.770972262236428E-5 0.0 7.164847298249213E-4 0.0 28-29 3.3938750360127854E-4 3.770972262236428E-5 0.0 0.0013009854304715676 0.0 30-31 3.770972262236428E-4 3.770972262236428E-5 0.0 0.003375020174701603 0.0 32-33 3.9595208753482496E-4 3.770972262236428E-5 0.0 0.008164154947741867 0.0 34-35 4.713715327795535E-4 3.770972262236428E-5 0.0 0.01534785710730226 0.0 36-37 5.279361167130999E-4 3.770972262236428E-5 0.0 0.028263437105462027 0.0 38-39 6.033555619578284E-4 3.770972262236428E-5 0.0 0.05087041581756942 0.0 40-41 6.033555619578284E-4 3.770972262236428E-5 0.0 0.07783286749255988 0.0 42-43 7.353395911361034E-4 7.541944524472856E-5 0.0 0.10415425388297014 0.0 44-45 8.296138976920141E-4 7.541944524472856E-5 0.0 0.13583042088575614 0.0 46-47 0.0010370173721150175 7.541944524472856E-5 0.0 0.17369098239860986 0.0 48-49 0.001093581956048564 7.541944524472856E-5 0.0 0.2174531155018636 0.0 50-51 0.001093581956048564 7.541944524472856E-5 0.0 0.2662872062978253 0.0 52-53 0.001093581956048564 7.541944524472856E-5 0.0 0.3158566366849232 0.0 54-55 0.001093581956048564 7.541944524472856E-5 0.0 0.3668401816703597 0.0 56-57 0.0011690014012932927 7.541944524472856E-5 0.0 0.4300605316467534 0.0 58-59 0.0011690014012932927 7.541944524472856E-5 0.0 0.5015016011548226 0.0 60-61 0.0011878562626044748 7.541944524472856E-5 0.0 0.5842178777269786 0.0 62-63 0.0012067111239156569 7.541944524472856E-5 0.0 0.6710256592036612 0.0 64-65 0.0012067111239156569 7.541944524472856E-5 0.0 0.7579277149868997 0.0 66-67 0.0013198402917827497 7.541944524472856E-5 0.0 0.8601964827387516 0.0 68-69 0.0013198402917827497 7.541944524472856E-5 0.0 0.9810938534660515 0.0 70-71 0.0013952597370274784 7.541944524472856E-5 0.0 1.1198090681324184 0.0 72-73 0.0014518243209610247 7.541944524472856E-5 0.0 1.2648218064767203 0.0 74-75 0.0014706791822722068 7.541944524472856E-5 0.0 1.4110224010836268 0.0 76-77 0.001508388904894571 7.541944524472856E-5 0.0 1.5763041153374493 0.0 78-79 0.0015460986275169354 7.541944524472856E-5 0.0 1.7654560840112286 0.0 80-81 0.0017912118245623032 7.541944524472856E-5 0.0 1.9815139397760646 0.0 82-83 0.0019231958537405782 7.541944524472856E-5 0.0 2.2084133407948308 0.0 84-85 0.0019986152989853066 7.541944524472856E-5 1.885486131118214E-5 2.4331632876241214 0.0 86-87 0.0020363250216076712 7.541944524472856E-5 3.770972262236428E-5 2.674486657545942 0.0 88-89 0.0020363250216076712 7.541944524472856E-5 3.770972262236428E-5 2.9532557820317695 0.0 90-91 0.0022248736347194923 7.541944524472856E-5 3.770972262236428E-5 3.2628337499000692 0.0 92-93 0.002262583357341857 7.541944524472856E-5 5.6564583933546416E-5 3.598035474290265 0.0 94-95 0.002262583357341857 9.427430655591069E-5 7.541944524472856E-5 3.933802844519797 0.0 96-97 0.002262583357341857 1.1312916786709283E-4 7.541944524472856E-5 4.271776233522736 0.0 98-99 0.0024322771091424963 1.1312916786709283E-4 7.541944524472856E-5 4.636316122113133 0.0 100-101 0.0026396805835655 1.1312916786709283E-4 7.541944524472856E-5 5.061116147454066 0.0 102-103 0.0027905194740549567 1.1312916786709283E-4 7.541944524472856E-5 5.512388398075899 0.0 104-105 0.0031864715615897814 1.1312916786709283E-4 7.541944524472856E-5 5.963773777865599 0.0 106-107 0.0034127298973239675 1.1312916786709283E-4 7.541944524472856E-5 6.4180250965746 0.0 108-109 0.003469294481257514 1.1312916786709283E-4 7.541944524472856E-5 6.896014685674379 0.0 110-111 0.003507004203879878 1.1312916786709283E-4 7.541944524472856E-5 7.429644970503455 0.0 112-113 0.0035447139265022422 1.1312916786709283E-4 7.541944524472856E-5 8.014522768376326 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3320 0.0 53.426006 1 GTATCAA 6225 0.0 52.206886 1 ATCAACG 6995 0.0 44.31601 3 CAACGCA 7285 0.0 42.757175 5 TCAACGC 7285 0.0 42.10394 4 AACGCAG 7240 0.0 42.01516 6 TATCAAC 8110 0.0 40.05661 2 GTACATG 10915 0.0 34.409637 1 ACGCAGA 8765 0.0 34.02782 7 CGCAGAG 8665 0.0 33.32315 8 TACATGG 11145 0.0 32.511696 2 ACATGGG 11430 0.0 29.515316 3 GCAGAGT 9655 0.0 27.99527 9 CATGGGA 6840 0.0 23.945377 4 GAGTACA 8780 0.0 22.507025 1 CAGAGTA 9845 0.0 22.153933 10-11 AGAGTAC 9115 0.0 22.100117 10-11 GAGTACT 4305 0.0 22.038574 12-13 CATGGGG 5490 0.0 21.80564 4 AGTACAT 8395 0.0 21.403692 2 >>END_MODULE