FastQCFastQC Report
Thu 26 May 2016
SRR1364479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2651836
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51910.19575117013269297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49070.18504160890794152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.10000618439451006No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.065.3493651
GTATCAA87400.049.70181
ATCAACG105750.038.83573
CAACGCA105900.038.1450275
TATCAAC112400.037.9168322
TCAACGC107750.037.67234
AACGCAG109700.036.552526
GTACATG110900.031.5833871
TACATGG112550.030.2400742
ACGCAGA134000.029.8801337
CGCAGAG134150.029.8786748
ACATGGG114550.027.630693
GCAGAGT147950.025.9260799
GAGTACT84500.025.76019912-13
GTACTTT91650.023.91299214-15
TGGTATC26500.023.8425272
GTGGTAT28300.023.8256191
CATGGGG51800.023.7044414
CAGAGTA145800.023.69973210-11
TAGTCGA1552.1090124E-423.0294575
Produced by FastQC (version 0.11.5)