##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364479_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2651836 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.127174531155017 31.0 28.0 31.0 25.0 34.0 2 29.28824331519747 31.0 28.0 33.0 25.0 34.0 3 29.381425548186236 31.0 28.0 34.0 26.0 34.0 4 32.84128467974641 35.0 32.0 37.0 27.0 37.0 5 32.046632597189266 35.0 32.0 37.0 22.0 37.0 6 32.11269248927913 35.0 32.0 37.0 23.0 37.0 7 31.59518688184337 35.0 32.0 37.0 18.0 37.0 8 31.69464778364876 35.0 32.0 37.0 19.0 37.0 9 32.25525371855575 37.0 31.0 39.0 16.0 39.0 10-11 32.46736789152874 37.0 31.5 39.0 17.0 39.0 12-13 32.24139388710313 37.0 31.0 39.0 16.0 39.0 14-15 33.210076151013865 38.0 32.0 40.0 17.0 41.0 16-17 33.267730545931194 38.0 32.0 40.0 17.0 41.0 18-19 33.10373152789237 38.0 31.5 40.0 17.0 41.0 20-21 33.00100986637183 38.0 31.0 40.0 14.0 41.0 22-23 32.986116788519354 38.0 31.0 40.0 16.5 41.0 24-25 32.80052801153616 38.0 31.0 40.0 13.0 41.0 26-27 32.877033308243796 37.5 31.0 40.0 15.5 41.0 28-29 32.7488783243006 37.0 31.0 40.0 12.5 41.0 30-31 32.26256638796667 37.0 30.0 40.0 9.5 41.0 32-33 32.199891132030785 37.0 30.0 40.0 9.0 41.0 34-35 31.999318208214987 37.0 30.0 40.0 9.0 41.0 36-37 31.982597717204232 37.0 30.0 40.0 8.0 41.0 38-39 31.759493045572952 37.0 30.0 40.0 8.0 41.0 40-41 31.471402831849332 36.5 30.0 40.0 4.5 41.0 42-43 31.354713677618072 36.0 30.0 40.0 2.0 41.0 44-45 31.280403652412893 36.0 30.0 40.0 2.0 41.0 46-47 31.136663240109872 36.0 29.5 40.0 2.0 41.0 48-49 30.866714608294025 36.0 29.0 40.0 2.0 41.0 50-51 30.51626703159622 36.0 28.0 40.0 2.0 41.0 52-53 30.415333565122427 36.0 28.0 40.0 2.0 41.0 54-55 29.937369052988196 35.0 27.0 39.5 2.0 41.0 56-57 29.958082249430205 35.0 28.0 39.0 2.0 41.0 58-59 29.718432436998366 35.0 27.0 39.0 2.0 41.0 60-61 29.431034950879315 35.0 26.5 39.0 2.0 41.0 62-63 29.164305598083743 34.5 26.0 39.0 2.0 40.5 64-65 28.690467283798846 34.0 26.0 38.0 2.0 40.0 66-67 28.292970794574025 34.0 25.0 38.0 2.0 40.0 68-69 28.10873881341078 34.0 24.5 37.5 2.0 40.0 70-71 28.17528780060305 34.0 26.0 37.0 2.0 40.0 72-73 27.944832749838227 34.0 25.5 37.0 2.0 39.0 74-75 27.59013943547037 34.0 24.5 36.0 2.0 39.0 76-77 27.50626697880261 34.0 25.5 36.0 2.0 39.0 78-79 27.10394779315161 33.0 24.5 35.5 2.0 37.5 80-81 26.871011631186846 33.0 24.0 35.0 2.0 37.0 82-83 26.72281902048241 33.0 24.0 35.0 2.0 37.0 84-85 26.29874773553116 33.0 22.5 35.0 2.0 36.0 86-87 26.167297676025214 33.0 22.5 35.0 2.0 36.0 88-89 26.13061478914986 33.0 23.0 35.0 2.0 36.0 90-91 25.97320724207681 33.0 23.0 35.0 2.0 35.0 92-93 25.981670812222173 33.0 23.0 35.0 2.0 35.0 94-95 25.98712929457176 33.0 23.0 35.0 2.0 35.0 96-97 25.870502738480056 33.0 23.0 35.0 2.0 35.0 98-99 25.731847859369886 33.0 21.5 35.0 2.0 35.0 100-101 25.642068928847785 33.0 20.5 35.0 2.0 35.0 102-103 24.47989638122418 30.5 19.0 34.0 2.0 35.0 104-105 25.44692092572844 32.5 21.0 35.0 2.0 35.0 106-107 25.561209667566168 33.0 20.0 35.0 2.0 35.0 108-109 25.634897293799465 33.0 21.5 35.0 2.0 35.0 110-111 25.564897866987252 33.0 20.0 35.0 2.0 35.0 112-113 25.51352383782406 33.0 20.0 35.0 2.0 35.0 114-115 25.457529047799337 33.0 19.5 35.0 2.0 35.0 116-117 25.37099635874918 33.0 19.0 35.0 2.0 35.0 118-119 25.24993947589519 33.0 18.5 35.0 2.0 35.0 120-121 25.081067607499108 33.0 17.0 35.0 2.0 35.0 122-123 24.78410844411193 33.0 2.0 35.0 2.0 35.0 124-125 23.99584288017811 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8626900060307818 1101 2 0.8459575172702678 1101 3 0.8773842454766907 1101 4 0.8550576056893533 1101 5 1.0599526359064484 1101 6 1.0747172823747846 1101 7 1.6412621659048519 1101 8 1.3730194010966699 1101 9 2.1739992764253557 1101 10-11 1.7569548065299756 1101 12-13 1.948807513958485 1101 14-15 1.8860275816718044 1101 16-17 1.7580069613924536 1101 18-19 1.8228803729321115 1101 20-21 2.08025760931433 1101 22-23 2.1680257783852 1101 24-25 2.2147807520454563 1101 26-27 2.114611074058118 1101 28-29 2.054663645522922 1101 30-31 2.578216554791041 1101 32-33 2.305054696924749 1101 34-35 2.4006505321076688 1101 36-37 2.253427985181119 1101 38-39 2.391040074385259 1101 40-41 2.599970921069165 1101 42-43 2.5828738722845976 1101 44-45 2.4119230106625267 1101 46-47 2.2390237578702283 1101 48-49 2.2053656004271858 1101 50-51 2.4353980710045775 1101 52-53 2.413012398237747 1101 54-55 2.6054077996213785 1101 56-57 2.4074597291264332 1101 58-59 2.595972695700617 1101 60-61 2.6649078658948113 1101 62-63 2.6028046622244005 1101 64-65 2.722714982011297 1101 66-67 2.8871360958740375 1101 68-69 2.7620879572128096 1101 70-71 2.4126737081843608 1101 72-73 2.604262604598354 1101 74-75 2.7376497966196816 1101 76-77 2.4898883536456253 1101 78-79 2.5672277731370308 1101 80-81 2.5665582779247025 1101 82-83 2.4738700494139714 1101 84-85 2.6941627956441927 1101 86-87 2.569750287303169 1101 88-89 2.4049243388246246 1101 90-91 2.4784510342166364 1101 92-93 2.3458516108466156 1101 94-95 2.226506609356388 1101 96-97 2.11947855679783 1101 98-99 2.224779735943901 1101 100-101 2.399501073366853 1101 102-103 2.2110372141224772 1101 104-105 2.2314065743802267 1101 106-107 2.346173155230808 1101 108-109 2.2937872393805954 1101 110-111 2.2504221076676707 1101 112-113 2.178737846522921 1101 114-115 2.2161105104544347 1101 116-117 2.2370364266805787 1101 118-119 2.0721994615225228 1101 120-121 2.0755567619780564 1101 122-123 2.226100215576391 1101 124-125 2.1414731205044824 1102 1 0.4424712626646148 1102 2 -1.5530735775145672 1102 3 0.02237926804325241 1102 4 0.4190506280528652 1102 5 0.9953240579229217 1102 6 0.49164867817897573 1102 7 1.4408057978885829 1102 8 1.0349441581253984 1102 9 1.8015208046490514 1102 10-11 1.4193089545450022 1102 12-13 1.6190272198482063 1102 14-15 1.7056673868992362 1102 16-17 1.5249955152733392 1102 18-19 1.7344836339091714 1102 20-21 1.884409856806819 1102 22-23 1.8625782378965852 1102 24-25 1.9611699833846927 1102 26-27 1.7281193932942216 1102 28-29 1.723240363264864 1102 30-31 2.0093851506801386 1102 32-33 1.6693641653875986 1102 34-35 1.963328483593834 1102 36-37 1.2716970513882373 1102 38-39 1.3105195469223645 1102 40-41 1.5385257117334774 1102 42-43 1.3399871982253977 1102 44-45 1.2607277541895527 1102 46-47 0.9724590835394125 1102 48-49 1.0012145923485818 1102 50-51 1.2645313119532275 1102 52-53 1.1623053653550421 1102 54-55 1.5262451160002364 1102 56-57 1.0953228081733464 1102 58-59 1.0960867217784198 1102 60-61 1.1447714248849792 1102 62-63 1.3571210793161832 1102 64-65 1.4808492421800956 1102 66-67 1.5563983413491869 1102 68-69 1.4575579521892301 1102 70-71 0.7937245729392544 1102 72-73 1.1431344478836962 1102 74-75 1.363933545925807 1102 76-77 1.270945837398095 1102 78-79 1.1336913956801276 1102 80-81 1.201308990917319 1102 82-83 0.8318068999577974 1102 84-85 0.8965328714462153 1102 86-87 0.9470867077440026 1102 88-89 1.1865376074095693 1102 90-91 1.0063000314121275 1102 92-93 0.9526651172935416 1102 94-95 0.9589026732943253 1102 96-97 1.023704799936553 1102 98-99 0.7998165953218468 1102 100-101 0.6057058166301736 1102 102-103 0.47943215995086064 1102 104-105 0.7728847204807572 1102 106-107 1.1148126875234716 1102 108-109 0.9595523599004103 1102 110-111 0.8659128826046114 1102 112-113 0.8988423264767214 1102 114-115 1.1490752807739 1102 116-117 0.7192687429473601 1102 118-119 0.24775244448849065 1102 120-121 0.6028816189510096 1102 122-123 0.9901729032454085 1102 124-125 1.0015180031095738 1103 1 0.7164356491267085 1103 2 0.6240674306887186 1103 3 0.5511682330803822 1103 4 0.5491025510553058 1103 5 0.995787742016681 1103 6 0.9417555881128337 1103 7 1.2396421917429983 1103 8 1.0530770804410352 1103 9 1.8929639997283374 1103 10-11 1.4261076380330024 1103 12-13 1.4370835994169582 1103 14-15 1.2149941684228196 1103 16-17 1.1536984694471712 1103 18-19 1.3258138403336588 1103 20-21 1.5018164023502862 1103 22-23 1.4458620776024276 1103 24-25 1.7102824135157277 1103 26-27 1.3040050782577595 1103 28-29 1.330151750096121 1103 30-31 1.9531715381388182 1103 32-33 1.7600153831738794 1103 34-35 1.8209554872535918 1103 36-37 1.782728992068371 1103 38-39 1.807011800000673 1103 40-41 2.027304752155729 1103 42-43 1.9364557070430344 1103 44-45 1.8545358649521475 1103 46-47 1.8065756498952936 1103 48-49 1.7787065126162673 1103 50-51 1.8652367764459683 1103 52-53 1.789795111443091 1103 54-55 2.047840637327564 1103 56-57 1.8597878260457357 1103 58-59 2.029051374478449 1103 60-61 1.9865414469791887 1103 62-63 1.9861662224181487 1103 64-65 2.0999573540680814 1103 66-67 2.2690905151848604 1103 68-69 2.292780497042447 1103 70-71 2.086174901916671 1103 72-73 2.2305666117620007 1103 74-75 2.263669284033906 1103 76-77 2.031556877507139 1103 78-79 2.2401634291520303 1103 80-81 2.0942087701126013 1103 82-83 2.0564397317398537 1103 84-85 2.1743588703972243 1103 86-87 2.2169513258315163 1103 88-89 2.065885827982232 1103 90-91 2.1470844764803836 1103 92-93 2.0421610082525454 1103 94-95 1.9067605885683143 1103 96-97 1.743248624819941 1103 98-99 1.9070241778036952 1103 100-101 1.9602053566709827 1103 102-103 1.831817911302167 1103 104-105 1.8428785107278465 1103 106-107 1.8137334015060986 1103 108-109 1.8274559683464595 1103 110-111 1.8092998867377759 1103 112-113 1.7645018539617041 1103 114-115 1.5847962543266263 1103 116-117 1.7081445779525417 1103 118-119 1.752213818826796 1103 120-121 1.7732946111088026 1103 122-123 1.9588264900685353 1103 124-125 1.734514980550781 1104 1 0.6187372729577056 1104 2 0.5629740116148128 1104 3 0.5402963349022301 1104 4 0.43184167175687804 1104 5 0.8590584305872966 1104 6 0.9720105772121315 1104 7 1.3238183761370017 1104 8 1.0217651848597384 1104 9 1.5819441567180306 1104 10-11 1.125465958429693 1104 12-13 1.2148367765834038 1104 14-15 1.2290329228645973 1104 16-17 0.9548413844159214 1104 18-19 0.9572889729286089 1104 20-21 1.4428427916986593 1104 22-23 1.5059132652701095 1104 24-25 1.6028852170622656 1104 26-27 1.3008307966566548 1104 28-29 1.35690674616923 1104 30-31 1.882746777728876 1104 32-33 1.6335094820876037 1104 34-35 1.6653868905309963 1104 36-37 1.568072168589616 1104 38-39 1.7059743549268447 1104 40-41 1.8188246555219862 1104 42-43 1.7501713489278323 1104 44-45 1.5141325316579426 1104 46-47 1.5583117991574227 1104 48-49 1.3932572440772333 1104 50-51 1.837842699637676 1104 52-53 1.6212331775560855 1104 54-55 1.8804951292503524 1104 56-57 1.7581613460079062 1104 58-59 1.8532651641930933 1104 60-61 1.8336826218100626 1104 62-63 1.8243613958083067 1104 64-65 2.025713687404327 1104 66-67 1.8835075404000037 1104 68-69 1.9523850907977192 1104 70-71 1.9378274458924807 1104 72-73 2.098368386758821 1104 74-75 2.183525459260629 1104 76-77 1.9919679381694344 1104 78-79 2.0738404602853677 1104 80-81 2.013056253379755 1104 82-83 1.9612188846433085 1104 84-85 2.2459603711984037 1104 86-87 2.077345298183374 1104 88-89 1.898850678943166 1104 90-91 2.0077459867518854 1104 92-93 1.752126347981303 1104 94-95 1.7767371345361198 1104 96-97 1.7690037590645176 1104 98-99 1.8047219418840683 1104 100-101 1.791174046130024 1104 102-103 1.7623274389634034 1104 104-105 1.622424806553088 1104 106-107 1.7781316677098644 1104 108-109 1.6915678523281699 1104 110-111 1.6998727886509712 1104 112-113 1.6497122323358333 1104 114-115 1.5684595084486688 1104 116-117 1.716620174229714 1104 118-119 1.6449558976222747 1104 120-121 1.704978395686915 1104 122-123 1.8340155978610575 1104 124-125 1.648351590213938 1105 1 0.3863154959378079 1105 2 0.5083933019602611 1105 3 0.40552496253282655 1105 4 0.6208277064920154 1105 5 0.7461917766184243 1105 6 0.845069557932554 1105 7 1.121588668921813 1105 8 0.9497253450477459 1105 9 1.4862893725513402 1105 10-11 1.1782284122965194 1105 12-13 1.2965586691478137 1105 14-15 1.2738661194994876 1105 16-17 1.1759085580699775 1105 18-19 1.4206311722875498 1105 20-21 1.5681058563240526 1105 22-23 1.4711276078797653 1105 24-25 1.6175778352241679 1105 26-27 1.353317236592872 1105 28-29 1.402560133442364 1105 30-31 1.7134670364376348 1105 32-33 1.6638451158429177 1105 34-35 1.8638607384404864 1105 36-37 1.596299920561684 1105 38-39 1.6459169558569897 1105 40-41 1.8365671919299302 1105 42-43 1.8023220755775888 1105 44-45 1.5911620344576463 1105 46-47 1.6704981047879386 1105 48-49 1.8261997259145488 1105 50-51 1.8880985452803287 1105 52-53 1.844696941321608 1105 54-55 1.9685916996625537 1105 56-57 1.7748937346469376 1105 58-59 1.7336954604283363 1105 60-61 1.530332096149845 1105 62-63 1.425743443544139 1105 64-65 1.89460967042789 1105 66-67 2.1116311167411546 1105 68-69 2.109411730475454 1105 70-71 1.9747431110442477 1105 72-73 2.0432070995938076 1105 74-75 2.09300395301376 1105 76-77 1.909020246021118 1105 78-79 2.004678934934706 1105 80-81 2.0162615632882357 1105 82-83 1.915713853583327 1105 84-85 2.0766791004616714 1105 86-87 2.014791691118724 1105 88-89 1.8671110652036091 1105 90-91 1.9793769140159405 1105 92-93 1.767704209196559 1105 94-95 1.5724707667163038 1105 96-97 1.6716734673564417 1105 98-99 1.7775631703510015 1105 100-101 1.763073544023264 1105 102-103 1.6139507312355477 1105 104-105 1.2369561090003245 1105 106-107 1.4499390216100956 1105 108-109 1.4360034237228874 1105 110-111 1.5421377608007916 1105 112-113 1.5742115122728926 1105 114-115 1.4588629618958144 1105 116-117 1.4497089934121448 1105 118-119 1.542977060253719 1105 120-121 1.528789894180516 1105 122-123 1.6224825285983862 1105 124-125 1.5567745626484317 1106 1 0.6189222385461619 1106 2 0.512304117420701 1106 3 0.5222950464358611 1106 4 0.4955881069397492 1106 5 0.7241875170708241 1106 6 0.9603851340761551 1106 7 1.1327830175869344 1106 8 0.9194030309029699 1106 9 1.6767612001674053 1106 10-11 1.213218925693468 1106 12-13 1.098686846138925 1106 14-15 1.2975116608484143 1106 16-17 1.2162927969062096 1106 18-19 1.2608117266360424 1106 20-21 1.0558734523497364 1106 22-23 1.1168266218384701 1106 24-25 0.9241527265104139 1106 26-27 0.9592886396153943 1106 28-29 0.9920830128043931 1106 30-31 1.3956247360306335 1106 32-33 1.2109586126822833 1106 34-35 1.2182653336839948 1106 36-37 1.353525445178164 1106 38-39 1.358673583652319 1106 40-41 1.5924343574983766 1106 42-43 1.5875489949131882 1106 44-45 1.4821390812760242 1106 46-47 1.4542441036089286 1106 48-49 1.3943702355629313 1106 50-51 1.7137822665417275 1106 52-53 1.493981598050027 1106 54-55 2.0389858544072688 1106 56-57 1.699590027228016 1106 58-59 1.6943363192067409 1106 60-61 1.8526477068473 1106 62-63 1.8558222577396961 1106 64-65 1.9693898346319827 1106 66-67 2.0924511372431063 1106 68-69 2.0653234233943216 1106 70-71 1.873285854682937 1106 72-73 1.8119726161058338 1106 74-75 2.1442026313812725 1106 76-77 1.7695871019661098 1106 78-79 1.7714989125061145 1106 80-81 1.8419874297784347 1106 82-83 1.7027549810441123 1106 84-85 2.0331059075496114 1106 86-87 2.049586739656416 1106 88-89 1.708720264276728 1106 90-91 1.8140415479388246 1106 92-93 1.7849721565197356 1106 94-95 1.6479763523988673 1106 96-97 1.5585613013639232 1106 98-99 1.5163841615020743 1106 100-101 1.21375839090258 1106 102-103 1.1064502940769572 1106 104-105 1.3570069241047555 1106 106-107 1.3315560843022993 1106 108-109 1.2880268135395525 1106 110-111 1.356173404669942 1106 112-113 1.0835427015222479 1106 114-115 1.1104286440569169 1106 116-117 1.4414154406374173 1106 118-119 1.1604370170509313 1106 120-121 1.1253365769583539 1106 122-123 1.3477767075307945 1106 124-125 1.2872538612261728 1107 1 0.4513197533528057 1107 2 0.46516488747169404 1107 3 0.32779145166806956 1107 4 0.36450022779995805 1107 5 0.47976838087082996 1107 6 0.6470198296558038 1107 7 0.8639345045855968 1107 8 0.7258585687456858 1107 9 1.4927141039585408 1107 10-11 0.9437425160271857 1107 12-13 1.2595978373657957 1107 14-15 1.0487363100814235 1107 16-17 1.107109775189798 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2216 122-123 -5.038913256806872 2216 124-125 -5.025987746998414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 113039.0 3 38806.0 4 19330.0 5 15819.0 6 16361.0 7 19328.0 8 21939.0 9 23333.0 10 25189.0 11 27135.0 12 29596.0 13 31553.0 14 32498.0 15 26654.0 16 19429.0 17 17032.0 18 15225.0 19 14109.0 20 14052.0 21 15518.0 22 18557.0 23 25302.0 24 35122.0 25 46254.0 26 56913.0 27 65196.0 28 74238.0 29 84163.0 30 93872.0 31 106963.0 32 124105.0 33 146809.0 34 177822.0 35 222322.0 36 310218.0 37 390990.0 38 136512.0 39 533.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.38343545197266 20.337570964306735 14.186670431548299 24.092323152172312 2 15.944623859465997 20.973456800365632 38.57693636238851 24.504982977779857 3 19.25058714038123 25.257067179116653 29.510007406189526 25.98233827431259 4 13.15239704939015 17.501282561768853 36.58920169879934 32.75711869004165 5 13.24674678222937 38.69096731472082 34.090004057566155 13.972281845483659 6 31.622968574178152 37.53454215802509 16.98339110471553 13.859098163081221 7 27.775608692486703 32.84063032486862 20.813938881589443 18.56982210105523 8 26.06220746682676 36.263403920906114 19.092583402593526 18.5818052096736 9 25.68092446139203 16.28664065198602 19.97434984667227 38.05808503994968 10-11 24.9392350717951 27.084537303189226 27.107823219358046 20.86840440565763 12-13 25.735184037824606 24.210645598360976 27.79039595085487 22.26377441295956 14-15 22.651939431066534 25.49504180428434 25.788764439838808 26.064254324810328 16-17 22.262537614587906 29.127846890926513 26.189916358393134 22.41969913609245 18-19 22.06663372043583 27.369625598957388 28.734625275591576 21.829115405015205 20-21 22.83103240606134 26.506105197421938 28.882680426600178 21.780181969916548 22-23 22.567319444135432 26.329466395806318 28.740529535254772 22.36268462480348 24-25 22.357061252473155 26.61757654414312 28.683291212896982 22.342070990486746 26-27 22.234641759209815 26.915820428579618 28.56389172979512 22.285646082415443 28-29 22.108245487571555 26.807955246318528 28.84439557961421 22.239403686495702 30-31 22.166321274101666 26.500166772663313 28.82076737296948 22.512744580265544 32-33 22.06983505192349 26.62023214495475 28.74053791818456 22.569394884937203 34-35 22.48690137966352 26.583769702610223 28.557292652629947 22.372036265096316 36-37 22.60172271372192 26.384316792584066 28.480248072586043 22.533712421107964 38-39 22.343828248028526 26.630292960618807 28.553572936967157 22.47230585438551 40-41 22.451965627862442 26.493014742067555 28.554671753025023 22.50034787704498 42-43 22.489192765021194 26.600589823220844 28.22570533388214 22.68451207787582 44-45 22.52084051397425 26.70131223777305 28.12539831345128 22.652448934801413 46-47 22.595525924054407 26.92194925584478 27.69062525630861 22.79189956379221 48-49 22.682212137375895 27.04794626057782 27.41614433937877 22.85369726266751 50-51 22.666195110909914 27.011864342839896 27.2783310698657 23.04360947638449 52-53 23.003486265171098 27.026115878250344 27.12578271272534 22.84461514385322 54-55 22.6599822593332 26.932828276916027 27.187364174199907 23.21982528955087 56-57 22.629094366738133 27.24930090716617 27.15001096412873 22.971593761966975 58-59 22.748427881663876 26.9505542575031 27.16862958342824 23.132388277404786 60-61 22.815758591406105 26.927117664893302 27.396867679600096 22.860256064100497 62-63 22.791701021996023 26.962797821345752 27.400704534865767 22.84479662179246 64-65 22.651572359315246 26.76422467304649 27.290145997449905 23.294056970188358 66-67 22.452414691270832 27.154750909979896 27.103706017638217 23.289128381111052 68-69 22.503680063474143 27.02421403516909 27.28922385778504 23.182882043571727 70-71 22.486761163565742 26.89441456389854 27.39204585629369 23.226778416242027 72-73 22.635381568038618 26.93110458106554 27.11512532391839 23.318388526977447 74-75 22.502013805504102 27.15558269122856 27.018198918729748 23.324204584537593 76-77 22.672822268773675 26.933669207722343 27.131403941118908 23.262104582385074 78-79 22.642522275469155 27.04764997005803 27.068013527435745 23.24181422703707 80-81 22.41426350052784 27.181365030168774 27.13004201058778 23.27432945871561 82-83 22.58589351504433 27.0688920481328 27.113012523427216 23.232201913395656 84-85 22.49411351229865 26.93893966293541 26.985058653702566 23.58188817106337 86-87 22.541215892879972 26.79494039774058 27.226359618210676 23.437484091168777 88-89 22.559776815874038 26.7998377706627 27.04331376236117 23.59707165110209 90-91 22.452055641701712 27.05954467566475 27.180518013261818 23.30788166937172 92-93 22.591695007837227 26.84395477505278 27.11047522472284 23.45387499238715 94-95 22.467007201000108 26.704302687128894 27.09578518053411 23.73290493133689 96-97 22.502439881618603 26.620541465165882 27.140609585755616 23.7364090674599 98-99 22.17688047073801 26.605246327450118 27.14164827689193 24.07622492491994 100-101 22.163712764485144 26.717538972540574 27.21955590215412 23.89919236082017 102-103 22.425473833185603 26.518669275457253 27.136607218445864 23.919249672911285 104-105 22.335931850899332 26.508254197804725 27.242633160276903 23.91318079101904 106-107 22.019088288093286 26.49840082463637 27.322829790912746 24.1596810963576 108-109 22.253431207661407 26.507521581274258 26.957059184655463 24.281988026408875 110-111 21.97091182383811 26.541579627831087 27.279672724550768 24.207835823780037 112-113 22.163227411670565 26.54421430602368 27.149832908093043 24.142725374212716 114-115 22.000413299183357 26.65955083127438 26.998448619671233 24.341587249871033 116-117 21.824916774642173 26.495020808224943 27.187031928067952 24.493030489064935 118-119 21.821189699231265 26.54890232996452 26.968067043728244 24.66184092707597 120-121 21.700869812467367 26.56283320797721 27.216997950620687 24.519299028934732 122-123 21.825528062058535 26.50688074864838 27.05201599355009 24.615575195743 124-125 21.748638949354888 26.501737800324932 26.97271261277977 24.776910637540407 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 137.0 1 110.0 2 141.0 3 230.5 4 209.5 5 190.5 6 377.5 7 786.5 8 1302.0 9 1605.5 10 1425.0 11 1112.5 12 1113.0 13 1272.5 14 1443.0 15 1661.5 16 1966.5 17 2235.0 18 2519.5 19 2890.5 20 3273.0 21 3849.5 22 4620.0 23 5732.0 24 7111.0 25 8935.5 26 12048.0 27 16135.5 28 20246.0 29 24592.0 30 30087.5 31 36182.5 32 41936.0 33 47506.5 34 53501.0 35 58295.0 36 63608.0 37 69774.0 38 73545.5 39 75337.0 40 76727.0 41 80102.5 42 84277.0 43 88567.0 44 93264.0 45 96821.0 46 97487.5 47 97440.5 48 100138.5 49 101559.5 50 100248.0 51 97710.5 52 94492.5 53 92877.5 54 90447.5 55 84391.5 56 77307.0 57 70641.0 58 65510.0 59 60131.5 60 51281.5 61 41261.5 62 32631.0 63 25078.0 64 18954.5 65 14338.0 66 10371.0 67 7791.5 68 5707.5 69 3930.5 70 2876.5 71 2112.5 72 1615.5 73 1306.5 74 1089.0 75 857.5 76 590.0 77 325.0 78 211.0 79 132.5 80 65.5 81 49.5 82 47.0 83 30.0 84 21.0 85 20.0 86 10.5 87 3.0 88 1.5 89 2.5 90 2.0 91 1.5 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10532325528426344 2 3.016777809789142E-4 3 0.0 4 0.1806295713611249 5 0.0 6 1.508388904894571E-4 7 0.0357865267686237 8 0.0 9 0.0 10-11 6.976298685137392E-4 12-13 6.410652845801928E-4 14-15 5.467909780242821E-4 16-17 0.0039783757366594315 18-19 0.0015272437662057533 20-21 0.002979068087166778 22-23 0.0014706791822722068 24-25 0.004317763240260709 26-27 0.003770972262236428 28-29 0.0028847937806108673 30-31 8.484687590031963E-4 32-33 5.656458393354642E-5 34-35 0.0011312916786709285 36-37 0.0011878562626044748 38-39 4.525166714683714E-4 40-41 0.0013952597370274784 42-43 9.238882042479249E-4 44-45 0.0011312916786709285 46-47 1.3198402917827498E-4 48-49 9.42743065559107E-5 50-51 0.039067272636769394 52-53 3.770972262236428E-5 54-55 0.13888490841816764 56-57 2.4511319704536785E-4 58-59 0.0 60-61 0.0 62-63 5.845007006466463E-4 64-65 0.05070072206576877 66-67 0.04630753938026333 68-69 0.001791211824562303 70-71 0.002771664612743774 72-73 0.07025321324546464 74-75 0.005279361167130999 76-77 0.0019231958537405782 78-79 0.0015083889048945712 80-81 1.6969375180063924E-4 82-83 2.262583357341857E-4 84-85 0.0 86-87 2.6396805835654996E-4 88-89 0.0013009854304715676 90-91 4.525166714683714E-4 92-93 0.0026208257222543174 94-95 0.004562876437306077 96-97 0.002564261138320771 98-99 0.0 100-101 0.001244420846538021 102-103 0.001225565985226839 104-105 1.3198402917827498E-4 106-107 1.885486131118214E-5 108-109 0.0 110-111 9.42743065559107E-5 112-113 7.541944524472856E-5 114-115 1.508388904894571E-4 116-117 0.0 118-119 5.467909780242821E-4 120-121 0.002168309050785946 122-123 6.410652845801928E-4 124-125 0.007843622305451771 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2651836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.31902966573769 #Duplication Level Percentage of deduplicated Percentage of total 1 76.89366518669235 42.536829455699355 2 11.800257883302775 13.055576318195625 3 4.425128673505452 7.343814730930637 4 2.233520686792509 4.942247885268544 5 1.2674616305384347 3.5057373769969966 6 0.7685369575714753 2.5508831253067332 7 0.5308189234173044 2.055507144015976 8 0.37797830796559384 1.6727514585083216 9 0.2831653795646853 1.4097990629203827 >10 1.2913650091434465 12.687591145919463 >50 0.08456429745566198 3.2377463366116515 >100 0.04214986993967229 4.066921030160108 >500 0.0011097552885421084 0.38960000467767725 >1k 2.0807911659522638E-4 0.3458208432215838 >5k 6.935970553174212E-5 0.1991740815669856 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5191 0.19575117013269297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4907 0.18504160890794152 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.10000618439451006 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.541944524472856E-5 0.0 0.0 0.0 0.0 3 7.541944524472856E-5 0.0 0.0 0.0 0.0 4 7.541944524472856E-5 0.0 0.0 0.0 0.0 5 7.541944524472856E-5 0.0 0.0 0.0 0.0 6 7.541944524472856E-5 0.0 0.0 0.0 0.0 7 1.508388904894571E-4 0.0 0.0 0.0 0.0 8 1.508388904894571E-4 0.0 0.0 0.0 0.0 9 1.508388904894571E-4 0.0 0.0 3.770972262236428E-5 0.0 10-11 1.508388904894571E-4 0.0 0.0 1.3198402917827498E-4 0.0 12-13 1.885486131118214E-4 0.0 0.0 1.885486131118214E-4 0.0 14-15 1.885486131118214E-4 0.0 0.0 1.885486131118214E-4 0.0 16-17 2.6396805835654996E-4 0.0 0.0 2.451131970453678E-4 0.0 18-19 3.5824236491246064E-4 0.0 0.0 3.016777809789142E-4 0.0 20-21 4.1480694884600707E-4 0.0 0.0 3.959520875348249E-4 0.0 22-23 4.5251667146837133E-4 0.0 0.0 5.090812554019177E-4 0.0 24-25 6.222104232690105E-4 0.0 0.0 6.222104232690105E-4 0.0 26-27 6.410652845801928E-4 0.0 0.0 8.484687590031962E-4 0.0 28-29 8.484687590031962E-4 0.0 0.0 0.0013198402917827497 0.0 30-31 9.050333429367427E-4 0.0 0.0 0.0032807458681456923 3.770972262236428E-5 32-33 9.050333429367427E-4 0.0 0.0 0.008145300086430683 3.770972262236428E-5 34-35 9.238882042479249E-4 0.0 0.0 0.015291292523368714 3.770972262236428E-5 36-37 0.0010558722334261998 0.0 0.0 0.028433130857262665 3.770972262236428E-5 38-39 0.0011312916786709285 0.0 0.0 0.05094583526281414 3.770972262236428E-5 40-41 0.0011501465399821106 1.885486131118214E-5 0.0 0.07766317374075923 3.770972262236428E-5 42-43 0.001244420846538021 3.770972262236428E-5 0.0 0.10421081846690369 3.770972262236428E-5 44-45 0.001357550014405114 3.770972262236428E-5 0.0 0.13664117992213695 3.770972262236428E-5 46-47 0.0014706791822722068 3.770972262236428E-5 0.0 0.17508624213563734 3.770972262236428E-5 48-49 0.0015838083501392996 3.770972262236428E-5 0.0 0.21881066551626874 3.770972262236428E-5 50-51 0.0015838083501392996 3.770972262236428E-5 0.0 0.26807841812238764 3.770972262236428E-5 52-53 0.0015838083501392996 3.770972262236428E-5 0.0 0.3171576221153948 3.770972262236428E-5 54-55 0.0015838083501392996 3.770972262236428E-5 0.0 0.36808460251689773 3.770972262236428E-5 56-57 0.001621518072761664 3.770972262236428E-5 0.0 0.4308524358218231 3.770972262236428E-5 58-59 0.001621518072761664 3.770972262236428E-5 0.0 0.501030229622043 3.770972262236428E-5 60-61 0.0016403729340728462 3.770972262236428E-5 0.0 0.581465067975546 3.770972262236428E-5 62-63 0.0016592277953840283 3.770972262236428E-5 0.0 0.6644264577447474 3.770972262236428E-5 64-65 0.0016592277953840283 3.770972262236428E-5 0.0 0.7478969287693508 3.770972262236428E-5 66-67 0.001734647240628757 3.770972262236428E-5 0.0 0.8459233527261867 3.770972262236428E-5 68-69 0.0017723569632511211 3.770972262236428E-5 0.0 0.9643318817604105 3.770972262236428E-5 70-71 0.0017912118245623032 5.6564583933546416E-5 0.0 1.0993138338871633 3.770972262236428E-5 72-73 0.0018100666858734853 9.427430655591069E-5 0.0 1.2391791950935125 3.770972262236428E-5 74-75 0.0018477764084958498 1.1312916786709283E-4 0.0 1.3774984576723448 3.770972262236428E-5 76-77 0.0018477764084958498 1.3198402917827498E-4 0.0 1.5355587600439846 3.770972262236428E-5 78-79 0.0018666312698070319 1.508388904894571E-4 0.0 1.7152455883395503 3.770972262236428E-5 80-81 0.0021305993281635818 1.508388904894571E-4 0.0 1.9201979307921002 3.770972262236428E-5 82-83 0.002187163912097128 1.508388904894571E-4 0.0 2.1366140289218487 3.770972262236428E-5 84-85 0.002187163912097128 1.508388904894571E-4 0.0 2.3523136423217723 3.770972262236428E-5 86-87 0.002187163912097128 1.508388904894571E-4 0.0 2.583210273938509 3.770972262236428E-5 88-89 0.002187163912097128 1.508388904894571E-4 0.0 2.850723046221561 3.770972262236428E-5 90-91 0.002262583357341857 1.508388904894571E-4 0.0 3.153155021652923 3.770972262236428E-5 92-93 0.002262583357341857 1.508388904894571E-4 0.0 3.4804377042924224 3.770972262236428E-5 94-95 0.002262583357341857 1.508388904894571E-4 0.0 3.8097567119535296 3.770972262236428E-5 96-97 0.002300293079964221 1.508388904894571E-4 0.0 4.14343119257752 3.770972262236428E-5 98-99 0.0024699868317648605 1.508388904894571E-4 0.0 4.505934756146308 3.770972262236428E-5 100-101 0.002583115999631953 1.508388904894571E-4 0.0 4.928905859940057 3.770972262236428E-5 102-103 0.0026019708609431353 1.508388904894571E-4 0.0 5.384420454356906 3.770972262236428E-5 104-105 0.002847084057988503 1.508388904894571E-4 0.0 5.840670388364892 3.770972262236428E-5 106-107 0.002960213225855596 1.508388904894571E-4 0.0 6.299805116153488 3.770972262236428E-5 108-109 0.003111052116345053 1.508388904894571E-4 0.0 6.780754918479122 3.770972262236428E-5 110-111 0.003205326422900964 1.508388904894571E-4 0.0 7.318702966548459 3.770972262236428E-5 112-113 0.003224181284212146 1.508388904894571E-4 0.0 7.902770005384949 3.770972262236428E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4885 0.0 65.349365 1 GTATCAA 8740 0.0 49.7018 1 ATCAACG 10575 0.0 38.8357 3 CAACGCA 10590 0.0 38.145027 5 TATCAAC 11240 0.0 37.916832 2 TCAACGC 10775 0.0 37.6723 4 AACGCAG 10970 0.0 36.55252 6 GTACATG 11090 0.0 31.583387 1 TACATGG 11255 0.0 30.240074 2 ACGCAGA 13400 0.0 29.880133 7 CGCAGAG 13415 0.0 29.878674 8 ACATGGG 11455 0.0 27.63069 3 GCAGAGT 14795 0.0 25.926079 9 GAGTACT 8450 0.0 25.760199 12-13 GTACTTT 9165 0.0 23.912992 14-15 TGGTATC 2650 0.0 23.842527 2 GTGGTAT 2830 0.0 23.825619 1 CATGGGG 5180 0.0 23.704441 4 CAGAGTA 14580 0.0 23.699732 10-11 TAGTCGA 155 2.1090124E-4 23.029457 5 >>END_MODULE