FastQCFastQC Report
Thu 26 May 2016
SRR1364478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834719
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63900.047.7897381
GGTATCA37500.047.6220551
ATCAACG69950.042.1047973
TCAACGC71500.040.7474984
CAACGCA72250.040.240475
TATCAAC79800.038.1006242
AACGCAG76250.037.250076
ACGCAGA88500.031.6246577
CGCAGAG88700.030.4148378
GTACATG97400.027.5635851
TACATGG94200.027.2864442
ACATGGG97850.024.201173
GCAGAGT108050.022.7082359
CAGAGTA97850.021.13731210-11
AGAGTAC90050.021.01837710-11
GAGTACT54800.018.8871612-13
GTATTAG17500.017.6881921
CGCATAG4101.0837539E-817.4304378
CATGGGG64900.017.075224
GTACTTT61650.016.91408314-15
Produced by FastQC (version 0.11.5)