FastQCFastQC Report
Thu 26 May 2016
SRR1364478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834719
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43050.1122637669148639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38910.10146766946939267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.059.7837681
GTATCAA82900.047.780381
ATCAACG93350.039.7642863
TCAACGC94750.038.7994354
CAACGCA95350.038.372665
TATCAAC101300.038.2904972
AACGCAG99950.036.4280666
ACGCAGA121650.029.6365767
CGCAGAG123700.029.566718
GTACATG99650.026.8785021
TACATGG98750.025.8241542
ACATGGG97050.024.5574863
GCAGAGT148750.023.5880819
GAGTACT89450.023.46885712-13
AGAGTAC125550.022.59431610-11
CAGAGTA136250.022.17301610-11
GTACTTT100750.020.98418414-15
TGGTATC25700.020.6336632
AGTACTT102950.019.32268112-13
GTGGTAT31700.018.4417821
Produced by FastQC (version 0.11.5)