##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364478_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3834719 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.931239551059672 31.0 28.0 31.0 25.0 34.0 2 29.16980096846731 31.0 28.0 34.0 25.0 34.0 3 29.260316075310865 31.0 28.0 34.0 25.0 34.0 4 32.68881918075353 35.0 32.0 37.0 26.0 37.0 5 31.804397923289816 35.0 32.0 37.0 22.0 37.0 6 31.8398677973536 35.0 32.0 37.0 22.0 37.0 7 31.29100932819328 35.0 31.0 37.0 17.0 37.0 8 31.413808156477696 35.0 32.0 37.0 17.0 37.0 9 32.044128917920716 37.0 30.0 39.0 11.0 39.0 10-11 32.22941263753616 37.0 31.5 39.0 16.5 39.0 12-13 31.98210390383233 37.0 30.5 39.0 13.5 39.0 14-15 32.94201230911574 38.0 31.0 40.0 14.0 41.0 16-17 32.988362771822395 38.0 31.0 40.0 14.5 41.0 18-19 32.83452568493284 38.0 31.0 40.0 11.0 41.0 20-21 32.70300757369706 38.0 31.0 40.0 10.5 41.0 22-23 32.67540033572212 38.0 31.0 40.0 10.0 41.0 24-25 32.47829215126323 38.0 30.5 40.0 10.0 41.0 26-27 32.56199137407461 37.5 31.0 40.0 10.0 41.0 28-29 32.4595452756773 37.0 30.5 40.0 9.5 41.0 30-31 31.964978920228575 37.0 30.0 40.0 8.0 41.0 32-33 31.934239901280904 37.0 30.0 40.0 8.0 41.0 34-35 31.747285133539123 37.0 30.0 40.0 8.0 41.0 36-37 31.750173480768733 37.0 30.0 40.0 4.0 41.0 38-39 31.54075800599731 37.0 30.0 40.0 2.0 41.0 40-41 31.267576450842945 37.0 29.5 40.0 2.0 41.0 42-43 31.173361724809563 36.5 29.5 40.0 2.0 41.0 44-45 31.125933608173113 37.0 29.0 40.0 2.0 41.0 46-47 31.037241711843812 37.0 29.0 40.0 2.0 41.0 48-49 30.80983235016699 36.0 29.0 40.0 2.0 41.0 50-51 30.48099939004657 36.0 28.0 40.0 2.0 41.0 52-53 30.39733380724898 36.0 28.0 40.0 2.0 41.0 54-55 29.93405149112621 35.5 27.0 40.0 2.0 41.0 56-57 29.957533003070107 35.0 27.5 40.0 2.0 41.0 58-59 29.718483544687366 35.0 27.0 39.0 2.0 41.0 60-61 29.442109969465818 35.0 26.5 39.0 2.0 41.0 62-63 29.175305022349747 35.0 26.0 39.0 2.0 41.0 64-65 28.698229779026832 34.0 26.0 39.0 2.0 40.0 66-67 28.303676097257714 34.0 24.5 38.0 2.0 40.0 68-69 28.11369907416945 34.0 24.5 38.0 2.0 40.0 70-71 28.197258391032044 34.0 26.0 37.0 2.0 40.0 72-73 27.970200554460444 34.0 25.5 37.0 2.0 39.5 74-75 27.597557865387266 34.0 24.5 36.0 2.0 39.0 76-77 27.505096722862874 34.0 25.5 36.0 2.0 39.0 78-79 27.098312027556645 33.5 24.0 35.5 2.0 38.5 80-81 26.864859850226317 33.0 24.0 35.0 2.0 37.0 82-83 26.715136363316322 33.0 24.0 35.0 2.0 37.0 84-85 26.273549900266488 33.0 22.0 35.0 2.0 36.0 86-87 26.140883204219136 33.0 22.5 35.0 2.0 36.0 88-89 26.093889148070563 33.0 23.0 35.0 2.0 36.0 90-91 25.92793591916383 33.0 22.0 35.0 2.0 35.5 92-93 25.944494498814645 33.0 23.0 35.0 2.0 35.0 94-95 25.956730727857767 33.0 23.0 35.0 2.0 35.0 96-97 25.83494266463853 33.0 23.0 35.0 2.0 35.0 98-99 25.697958051163592 33.0 21.5 35.0 2.0 35.0 100-101 25.600718331643073 33.0 20.0 35.0 2.0 35.0 102-103 24.469581343509134 30.5 19.0 34.0 2.0 35.0 104-105 25.427965386772797 32.5 20.0 35.0 2.0 35.0 106-107 25.533079477270693 33.0 20.0 35.0 2.0 35.0 108-109 25.607863966042885 33.0 20.0 35.0 2.0 35.0 110-111 25.535435842887054 33.0 20.0 35.0 2.0 35.0 112-113 25.48489289567241 33.0 19.5 35.0 2.0 35.0 114-115 25.43751589099488 33.0 19.0 35.0 2.0 35.0 116-117 25.360014645140883 33.0 19.0 35.0 2.0 35.0 118-119 25.236588130707883 33.0 18.0 35.0 2.0 35.0 120-121 25.077217913489882 33.0 15.0 35.0 2.0 35.0 122-123 24.787827087199872 33.0 2.0 35.0 2.0 35.0 124-125 24.016194667718807 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9058821254918072 1101 2 0.8678045773883092 1101 3 0.7836107266265024 1101 4 0.9103112697921958 1101 5 1.0289821665818195 1101 6 1.1073461203081223 1101 7 1.5566855635530779 1101 8 1.18092812852408 1101 9 1.9722031114531546 1101 10-11 1.5900331098170497 1101 12-13 1.8810292861687756 1101 14-15 1.792638560858066 1101 16-17 1.673153787426216 1101 18-19 1.736831296645498 1101 20-21 1.9231572396231726 1101 22-23 1.8505397646443171 1101 24-25 2.019140216281336 1101 26-27 1.7835111096017897 1101 28-29 1.8317591469749317 1101 30-31 2.265352678504737 1101 32-33 2.0912562201521716 1101 34-35 2.148062879118278 1101 36-37 2.005234923930903 1101 38-39 2.1603066981253534 1101 40-41 2.303253075175597 1101 42-43 2.2734971973689255 1101 44-45 2.062632338455515 1101 46-47 1.8968089147647866 1101 48-49 1.993776242617944 1101 50-51 2.336452772365739 1101 52-53 2.219808717823817 1101 54-55 2.5781208855731386 1101 56-57 2.1248267413698265 1101 58-59 2.323760793424242 1101 60-61 2.3725038560005878 1101 62-63 2.3647451447476087 1101 64-65 2.4793051111225672 1101 66-67 2.633321160188043 1101 68-69 2.6263531647406424 1101 70-71 2.1818288564326345 1101 72-73 2.45231956417242 1101 74-75 2.5152812842236614 1101 76-77 2.222784163402281 1101 78-79 2.40982220298444 1101 80-81 2.4085266036926356 1101 82-83 2.291039331878565 1101 84-85 2.480742227549321 1101 86-87 2.3645478200531933 1101 88-89 2.338405878033136 1101 90-91 2.3662415948957616 1101 92-93 2.181676749758001 1101 94-95 2.1099008497378513 1101 96-97 2.0444776194314223 1101 98-99 2.089996407414276 1101 100-101 2.1516004453240534 1101 102-103 1.9527026658313922 1101 104-105 2.0353415465262685 1101 106-107 2.1841492349538996 1101 108-109 2.149868695061837 1101 110-111 2.1691920387921506 1101 112-113 2.104020245551748 1101 114-115 2.1577652410561328 1101 116-117 2.1132234748859773 1101 118-119 2.0094633756118014 1101 120-121 1.9461234898049824 1101 122-123 2.080537939321154 1101 124-125 2.007308237138787 1102 1 0.562431202203296 1102 2 -1.6549361720636924 1102 3 0.02510004414443756 1102 4 0.6229017595604844 1102 5 1.0945446573694753 1102 6 0.6325026201206541 1102 7 1.42751664623405 1102 8 1.167848518294555 1102 9 2.0098667864545163 1102 10-11 1.6647411871328046 1102 12-13 1.8029134032859133 1102 14-15 1.8177257353110363 1102 16-17 1.7699738329004902 1102 18-19 1.908012510483168 1102 20-21 2.1410829605335593 1102 22-23 2.006043853965849 1102 24-25 2.1591808849017227 1102 26-27 1.841197116567173 1102 28-29 1.8228722114729692 1102 30-31 2.166990828004625 1102 32-33 1.8860130052341937 1102 34-35 2.1764231021512472 1102 36-37 1.4581109754924917 1102 38-39 1.488774032647946 1102 40-41 1.6800746363838144 1102 42-43 1.496933762379463 1102 44-45 1.4709974743625622 1102 46-47 1.1847592305182424 1102 48-49 1.3546971565186148 1102 50-51 1.4747578240372583 1102 52-53 1.426878064852854 1102 54-55 1.6890696481225298 1102 56-57 1.2525454692277513 1102 58-59 1.191466620050008 1102 60-61 1.1443550287588202 1102 62-63 1.5719796932338284 1102 64-65 1.657617582328701 1102 66-67 1.723117533803034 1102 68-69 1.6556981351787456 1102 70-71 1.0563005221396011 1102 72-73 1.4898478332313339 1102 74-75 1.6916449707594943 1102 76-77 1.5970567691125943 1102 78-79 1.4491527056686309 1102 80-81 1.5029709365293016 1102 82-83 1.0617903278275023 1102 84-85 1.103980031646973 1102 86-87 1.2267926592781748 1102 88-89 1.3529684640761523 1102 90-91 1.192150070297643 1102 92-93 1.1969438755765616 1102 94-95 1.2298640251903201 1102 96-97 1.2869355451173483 1102 98-99 0.9894636320094783 1102 100-101 0.8957430026414848 1102 102-103 0.7051855971773335 1102 104-105 1.0493089558762385 1102 106-107 1.2657323451406484 1102 108-109 1.18924228273751 1102 110-111 1.181151975336732 1102 112-113 1.1334075109487465 1102 114-115 1.3864832021310036 1102 116-117 0.9861363216510277 1102 118-119 0.5296947276261719 1102 120-121 0.852953028085107 1102 122-123 1.1556976049071324 1102 124-125 1.1949806476433338 1103 1 0.7811038709955191 1103 2 0.7341790996471964 1103 3 0.6453567122624762 1103 4 0.6484630893917966 1103 5 1.0222881016157217 1103 6 1.1363396076341772 1103 7 1.2682937857202639 1103 8 1.2293086079050752 1103 9 2.055118861682402 1103 10-11 1.6835615868687483 1103 12-13 1.6820649686608604 1103 14-15 1.4622319504605485 1103 16-17 1.5271230245799146 1103 18-19 1.7272523380039857 1103 20-21 1.8464363743009855 1103 22-23 1.691989273014407 1103 24-25 2.051214004274726 1103 26-27 1.5990145667406352 1103 28-29 1.678015504027428 1103 30-31 2.2792292790629567 1103 32-33 2.0668892078831824 1103 34-35 2.1452534601719506 1103 36-37 1.9942181569748563 1103 38-39 2.0627620096694557 1103 40-41 2.344066349517796 1103 42-43 2.2599492826963257 1103 44-45 2.1616103372640936 1103 46-47 2.187075325297517 1103 48-49 2.190726062230933 1103 50-51 2.3266985940135214 1103 52-53 2.214802780781895 1103 54-55 2.4535982518558335 1103 56-57 2.223666682200207 1103 58-59 2.4233832457505287 1103 60-61 2.3287601862794993 1103 62-63 2.4524471146139426 1103 64-65 2.6042618650934237 1103 66-67 2.844673119010274 1103 68-69 2.958499629623738 1103 70-71 2.6050310304475346 1103 72-73 2.6423112375882596 1103 74-75 2.6314855398846007 1103 76-77 2.534748567924339 1103 78-79 2.6031840412586114 1103 80-81 2.5320975570329765 1103 82-83 2.4568830143171 1103 84-85 2.6528315843809374 1103 86-87 2.4813447892836535 1103 88-89 2.438927802250511 1103 90-91 2.4816188681402522 1103 92-93 2.3016959999638225 1103 94-95 2.175574546701057 1103 96-97 2.089997261200889 1103 98-99 2.283332502888868 1103 100-101 2.2600917673366254 1103 102-103 2.1524591199381256 1103 104-105 2.147919641719742 1103 106-107 2.176250934407051 1103 108-109 2.14456590592388 1103 110-111 2.1469672344066346 1103 112-113 2.0276642773855365 1103 114-115 1.9162862415849133 1103 116-117 1.9995837000518044 1103 118-119 2.040398563461949 1103 120-121 1.99792950181293 1103 122-123 2.1253666218600955 1103 124-125 2.027332038853636 1104 1 0.6210518007299441 1104 2 0.6136726391141814 1104 3 0.6371824525700127 1104 4 0.6180387207204703 1104 5 0.9062820187651681 1104 6 1.0127846939354512 1104 7 1.3644408922067548 1104 8 1.1647350097392462 1104 9 1.8112889552212081 1104 10-11 1.2858509215819254 1104 12-13 1.3247159867397045 1104 14-15 1.3643911494409764 1104 16-17 1.228574403370466 1104 18-19 1.1768250809769327 1104 20-21 1.4868893536665482 1104 22-23 1.518865569530071 1104 24-25 1.7357934057728173 1104 26-27 1.6236839065783073 1104 28-29 1.559061394942077 1104 30-31 2.060128941977208 1104 32-33 1.927173158541546 1104 34-35 1.8042533170852053 1104 36-37 1.7557001097429215 1104 38-39 1.854350199355892 1104 40-41 2.010090075297036 1104 42-43 1.9505096303638538 1104 44-45 1.6771488791768618 1104 46-47 1.7568185877419076 1104 48-49 1.4437832936022055 1104 50-51 1.9433845984658475 1104 52-53 1.6720999187141956 1104 54-55 1.9513293352887153 1104 56-57 1.9309201420710274 1104 58-59 1.9946309688410722 1104 60-61 2.102035961027685 1104 62-63 2.1231958443031083 1104 64-65 2.320024740404971 1104 66-67 2.200894737088454 1104 68-69 2.3524880296144204 1104 70-71 2.1332934049554666 1104 72-73 2.25635459620025 1104 74-75 2.386426378506041 1104 76-77 2.1333884185817062 1104 78-79 2.2318653338310774 1104 80-81 2.0571580095198563 1104 82-83 2.090081788429714 1104 84-85 2.3847747889251067 1104 86-87 2.2491541711156273 1104 88-89 2.0751813519665028 1104 90-91 2.1543822725400013 1104 92-93 1.9092834841993245 1104 94-95 1.8408451570159485 1104 96-97 1.8603810997191772 1104 98-99 1.91710612634445 1104 100-101 1.986385108478725 1104 102-103 1.975570997136419 1104 104-105 1.7789413143705417 1104 106-107 1.8702209527421196 1104 108-109 1.7609478876420503 1104 110-111 1.790584853376611 1104 112-113 1.8076435664107855 1104 114-115 1.7338016167550379 1104 116-117 1.7238120531898957 1104 118-119 1.6447227248238683 1104 120-121 1.6393007387146703 1104 122-123 1.7621429107222895 1104 124-125 1.5248430215933837 1105 1 0.5837658266051307 1105 2 0.620349451923282 1105 3 0.4311412697345425 1105 4 0.6033892809438299 1105 5 0.8450554172984468 1105 6 1.0138980751363746 1105 7 1.3763118462822668 1105 8 1.1637502083508124 1105 9 1.75514050989554 1105 10-11 1.4450236770152785 1105 12-13 1.58901340636789 1105 14-15 1.4366950904027291 1105 16-17 1.483670508297891 1105 18-19 1.650007110497306 1105 20-21 1.8446888620448831 1105 22-23 1.800416638367075 1105 24-25 1.8674491177307928 1105 26-27 1.6943605542450086 1105 28-29 1.7030566023378952 1105 30-31 2.0239770597134275 1105 32-33 1.9446498748601435 1105 34-35 2.0443869201149454 1105 36-37 1.7581618824923702 1105 38-39 1.8383025593003168 1105 40-41 2.044408154826577 1105 42-43 2.0835846197011136 1105 44-45 1.9436115535321932 1105 46-47 1.8683683881527493 1105 48-49 1.9024790171168995 1105 50-51 2.0930345082360446 1105 52-53 2.0914010216097765 1105 54-55 2.2383494027240367 1105 56-57 2.096292672966083 1105 58-59 2.046124428656185 1105 60-61 1.9330543736731194 1105 62-63 1.7919295687575776 1105 64-65 2.1937970264527884 1105 66-67 2.3645156428684935 1105 68-69 2.3084238608777525 1105 70-71 2.2034097790503573 1105 72-73 2.416980490806445 1105 74-75 2.4164623322662777 1105 76-77 2.2763194834888374 1105 78-79 2.4081016509860405 1105 80-81 2.310000439044778 1105 82-83 2.2510574434694846 1105 84-85 2.4888789984522752 1105 86-87 2.4239525224385083 1105 88-89 2.2167520820873 1105 90-91 2.2256500154751606 1105 92-93 2.0096322240289446 1105 94-95 1.9201569284801394 1105 96-97 1.9978930154739665 1105 98-99 2.0414653343134326 1105 100-101 2.0287755274396346 1105 102-103 1.7913985477485355 1105 104-105 1.4870323775415564 1105 106-107 1.6490963970136008 1105 108-109 1.693260306674027 1105 110-111 1.8643967095427882 1105 112-113 1.878726962756005 1105 114-115 1.741990172033404 1105 116-117 1.777494599920324 1105 118-119 1.8592189057157142 1105 120-121 1.9054433207248813 1105 122-123 1.9830289409911757 1105 124-125 1.8634250731707453 1106 1 0.4828303509127636 1106 2 0.38884598553548955 1106 3 0.42311700711556455 1106 4 0.24129988574175343 1106 5 0.4749506167728441 1106 6 0.6467521142279509 1106 7 0.878294539294604 1106 8 0.5399831117448741 1106 9 1.1851178614837323 1106 10-11 0.8597876317680786 1106 12-13 0.8392330225893829 1106 14-15 0.987966774500947 1106 16-17 0.8725363738068168 1106 18-19 1.013026745249256 1106 20-21 0.8053364571941657 1106 22-23 0.7444859470112917 1106 24-25 0.6645856602885942 1106 26-27 0.7244948196675836 1106 28-29 0.6269624455431995 1106 30-31 1.031473642669436 1106 32-33 0.906258849798359 1106 34-35 0.9050008072477453 1106 36-37 0.9089086502920232 1106 38-39 1.0977395326020911 1106 40-41 1.3457798816577409 1106 42-43 1.3482758133085753 1106 44-45 1.248714953934524 1106 46-47 1.2746609964239717 1106 48-49 1.1726830947921947 1106 50-51 1.5374008156876897 1106 52-53 1.1063297416165838 1106 54-55 1.6366892493827372 1106 56-57 1.3124596205235726 1106 58-59 1.2676535186129456 1106 60-61 1.5087271523206987 1106 62-63 1.6042865073700376 1106 64-65 1.6821359359705141 1106 66-67 1.818582155736749 1106 68-69 1.6654534492180417 1106 70-71 1.560088624079146 1106 72-73 1.5113012013481821 1106 74-75 1.7836714398236317 1106 76-77 1.2943780559733362 1106 78-79 1.3028463577461338 1106 80-81 1.5052493885611575 1106 82-83 1.288755084796854 1106 84-85 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-4.278660241616116 2216 106-107 -4.394097916525325 2216 108-109 -4.471423556253786 2216 110-111 -4.105272478479623 2216 112-113 -4.162510950993063 2216 114-115 -4.103747603269191 2216 116-117 -3.90286400261909 2216 118-119 -4.12425429139148 2216 120-121 -4.05958198563874 2216 122-123 -4.1250443467618325 2216 124-125 -4.0791241379289715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 192816.0 3 63579.0 4 29933.0 5 24159.0 6 23763.0 7 27376.0 8 30860.0 9 32523.0 10 34856.0 11 37227.0 12 40111.0 13 41999.0 14 41040.0 15 34789.0 16 27333.0 17 23924.0 18 20958.0 19 19863.0 20 19491.0 21 21192.0 22 26088.0 23 35377.0 24 48811.0 25 63890.0 26 78994.0 27 91316.0 28 103807.0 29 117379.0 30 131064.0 31 149166.0 32 174635.0 33 207294.0 34 251096.0 35 316990.0 36 446716.0 37 586643.0 38 216636.0 39 1025.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.70591429358318 19.931290765108134 14.515896189535155 24.84689875177353 2 15.644898032626742 20.844773798884763 38.815690481697814 24.69463768679067 3 19.17574664532134 24.406012539641107 29.62529979380497 26.792941021232586 4 12.949457801913203 17.514914333198625 36.95128746412318 32.58434040076499 5 13.229235310331736 38.19792271611036 34.78348739503468 13.789354578523225 6 31.7407586956312 37.736331245649936 17.165482788412067 13.357427270306799 7 27.51178622634146 32.142667078497624 21.24765997232663 19.097886722834286 8 25.29285718197344 36.59107746877933 19.54636050255573 18.569704846691504 9 24.89606148455728 16.443082270174163 20.05489841628552 38.60595782898304 10-11 24.502032239406304 26.946730449377082 27.798429910558518 20.7528074006581 12-13 25.34688905222232 23.94146960713789 28.054838309678885 22.6568030309609 14-15 22.185052811899357 25.609810638109504 25.723470279053522 26.48166627093762 16-17 22.09713868529763 28.66394831099411 26.519967083478015 22.718945920230244 18-19 22.055205784618032 27.60057136859207 28.287674046083705 22.056548800706192 20-21 22.682774620258876 26.853446499830753 28.473699637979173 21.990079241931202 22-23 22.2047151692008 26.671908351359775 28.338320757201775 22.78505572223765 24-25 22.13320210461448 26.831031158605896 28.413638554851556 22.62212818192807 26-27 22.08970049806372 27.085828465638535 28.269213893313932 22.555257142983812 28-29 22.048636827358585 27.245928111106704 28.256063760762572 22.449371300772132 30-31 22.13134419280004 26.965857456982157 28.16266929669376 22.74012905352404 32-33 22.02157865145685 26.870399268159623 28.286579762360496 22.82144231802303 34-35 22.43627514704983 27.04728530059761 28.11881180372911 22.397627748623446 36-37 22.51431480230226 26.738071389249708 28.166630896502877 22.580982911945156 38-39 22.229267538338732 27.087054290310657 28.12602762302856 22.557650548322044 40-41 22.420713206272794 26.988099320538616 28.043670160679383 22.547517312509207 42-43 22.42540715219298 26.873166080959997 27.919093202986218 22.782333563860803 44-45 22.42742257145022 27.145064627939668 27.87765499628978 22.549857804320332 46-47 22.586388540463297 27.13292530595534 27.515170638718445 22.765515514862923 48-49 22.555223762275027 27.248464489035307 27.467620165973134 22.728691582716536 50-51 22.60709307150295 27.360721190111864 27.222873618377452 22.80931212000774 52-53 22.691497884067555 27.304838220092442 27.19301768370852 22.810646212131484 54-55 22.577229430966874 27.313684103875907 27.162477598431767 22.946608866725455 56-57 22.54261730352491 27.48875403876695 27.167947511024305 22.800681146683843 58-59 22.53582335498377 27.31567815008088 27.290526372336537 22.857972122598813 60-61 22.5719928891791 27.349657171751048 27.356841531282996 22.721508407786857 62-63 22.496443976378277 27.30389845021391 27.43260505639243 22.767052517015387 64-65 22.390140589828324 27.245073706686956 27.278591275751218 23.086194427733496 66-67 22.34048701234744 27.433466923697992 27.106963914686023 23.119082149268543 68-69 22.47894691891899 27.17934675958252 27.35715948185657 22.984546839641922 70-71 22.45531609277834 27.102369424519157 27.337269943529996 23.105044539172514 72-73 22.505018172936207 27.11186011118789 27.193721829861232 23.189399886014666 74-75 22.299690640657445 27.395689832215748 27.09630561703986 23.20831391008694 76-77 22.520908627907396 27.099957466905156 27.2217674521946 23.157366452992846 78-79 22.482556489955837 27.277331208158 27.180999430458574 23.059112871427587 80-81 22.49710766802744 27.210138658518634 27.068889330179857 23.223864343274073 82-83 22.54520154566938 27.2894406206043 27.177567870772112 22.987789962954214 84-85 22.358887313516323 27.27617069203767 27.07775980456456 23.28718218988145 86-87 22.49248020156932 27.193434810789498 27.165935994386015 23.148148993255163 88-89 22.49922711722614 27.124593136383723 27.084185481956137 23.291994264434003 90-91 22.38847129950964 27.252296654660533 27.18188712152268 23.177344924307146 92-93 22.450256621823588 27.236740640755265 27.213635418896892 23.099367318524262 94-95 22.356239489560853 27.144924979407786 27.224086181004676 23.274749350026685 96-97 22.47715387890203 27.003218700145844 27.23298056066797 23.28664686028416 98-99 22.14588604797379 26.988053101152914 27.202984103920002 23.663076746953298 100-101 22.26760208023251 27.010112350027743 27.294191738918695 23.428093830821055 102-103 22.16337887274855 26.805781002687716 27.409067314511432 23.621772810052303 104-105 22.176571991623366 26.83973652790489 27.30769847604 23.675993004431753 106-107 21.937729148355334 26.870598353385017 27.22003757514736 23.97163492311229 108-109 22.145054454453437 26.871203192620268 27.146216860507494 23.837525492418806 110-111 22.002172521578856 26.88663002160917 27.181254301982587 23.929943154829388 112-113 22.141071761525954 26.89717488783391 27.207458702928378 23.75429464771176 114-115 22.038575251730084 26.97552945624998 27.10809480502124 23.877800486998698 116-117 21.88475870070271 26.867014245372346 27.299040685901627 23.949186368023316 118-119 21.915214373652265 26.884921126465994 27.101379696835437 24.0984848030463 120-121 21.835178791229424 26.941851125529386 27.221851334153925 24.001118749087265 122-123 21.79567986840163 26.949788483995352 27.09637108319873 24.15816056440429 124-125 21.768471525991245 26.858073372208242 27.09756103603777 24.275894065762742 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 50.5 2 73.5 3 123.5 4 110.5 5 113.5 6 220.0 7 486.0 8 842.5 9 1023.0 10 916.5 11 790.5 12 815.0 13 969.5 14 1177.0 15 1349.5 16 1590.0 17 1931.0 18 2267.5 19 2616.0 20 3245.0 21 4256.0 22 5562.5 23 7335.5 24 9779.0 25 13135.0 26 18364.5 27 24897.0 28 30962.0 29 37467.0 30 44887.0 31 53722.0 32 63627.5 33 71346.0 34 77997.0 35 86056.5 36 95177.0 37 103140.0 38 108601.0 39 113260.5 40 118855.0 41 124685.0 42 128688.0 43 132348.0 44 137293.5 45 141501.5 46 144146.5 47 146389.0 48 147482.0 49 147292.0 50 144348.0 51 140818.0 52 139033.0 53 135307.5 54 130158.0 55 121589.5 56 110424.0 57 99873.5 58 90037.5 59 79282.0 60 66670.5 61 54755.0 62 43195.0 63 32155.5 64 23118.5 65 16580.5 66 12237.5 67 9299.5 68 7084.0 69 5544.0 70 4200.0 71 2913.5 72 2090.5 73 1601.0 74 1135.0 75 786.5 76 565.0 77 345.0 78 210.0 79 126.0 80 80.5 81 58.0 82 34.0 83 23.0 84 16.5 85 7.5 86 4.5 87 5.5 88 3.5 89 2.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10650063277126694 2 3.129303607382966E-4 3 0.0 4 0.17445867611160035 5 0.0 6 1.564651803691483E-4 7 0.03731694551804187 8 0.0 9 0.0 10-11 8.605584920303157E-4 12-13 7.171320766919297E-4 14-15 6.388994865073556E-4 16-17 0.0044462188754899636 18-19 0.0017341557490913935 20-21 0.003468311498182787 22-23 0.0019297372245528292 24-25 0.005019924536843508 26-27 0.004420141345428439 28-29 0.0035204665583058363 30-31 8.084034319072661E-4 32-33 7.823259018457415E-5 34-35 0.0012908377380454735 36-37 0.0013560315631992853 38-39 5.215506012304944E-4 40-41 0.0016689619239375819 42-43 9.127135521533651E-4 44-45 0.0010039849073687015 46-47 2.3469777055372244E-4 48-49 5.2155060123049434E-5 50-51 0.039103256327256315 52-53 7.823259018457415E-5 54-55 0.1352902259591902 56-57 2.3469777055372244E-4 58-59 0.0 60-61 0.0 62-63 7.432096067534544E-4 64-65 0.06259911091269008 66-67 0.051698703346972755 68-69 0.0017341557490913935 70-71 0.003051071017198392 72-73 0.06902722207285593 74-75 0.004733071706166736 76-77 0.0015124967435684335 78-79 0.0014342641533838594 80-81 2.7381406564600956E-4 82-83 1.303876503076236E-4 84-85 0.0 86-87 2.216590055229601E-4 88-89 0.0012908377380454735 90-91 2.868528306767719E-4 92-93 0.0022948226454141754 94-95 0.003611737913521173 96-97 0.002516481650937135 98-99 0.0 100-101 0.001342992798168523 102-103 0.0014473029184146218 104-105 1.4342641533838596E-4 106-107 9.12713552153365E-5 108-109 1.3038765030762358E-5 110-111 1.1734888527686122E-4 112-113 1.0431012024609887E-4 114-115 1.6950394539991067E-4 116-117 0.0 118-119 9.387910822148898E-4 120-121 0.0020601248748604527 122-123 5.476281312920191E-4 124-125 0.007392979772442257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3834719.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.217162094471036 #Duplication Level Percentage of deduplicated Percentage of total 1 78.8815836678664 48.28906693664356 2 11.444775267395993 14.01233325357947 3 4.137900738539839 7.599316207260742 4 1.941352368169527 4.753763304188766 5 1.0665640566274284 3.264601236934893 6 0.6223653383012732 2.2859663878061607 7 0.4119650008235808 1.765352976286623 8 0.2768609260576491 1.3558912150477174 9 0.1975703256720307 1.0885225186549923 >10 0.9385455170326606 10.167541541068973 >50 0.058702417710594236 2.449565092415602 >100 0.02043574537997705 2.1267846693261223 >500 0.0010339728317650435 0.4169682089749863 >1k 3.4465759120105085E-4 0.4243264518114653 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4305 0.1122637669148639 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3891 0.10146766946939267 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2155060123049434E-5 2 0.0 0.0 0.0 0.0 5.2155060123049434E-5 3 0.0 0.0 0.0 0.0 5.2155060123049434E-5 4 0.0 0.0 0.0 0.0 5.2155060123049434E-5 5 0.0 0.0 0.0 0.0 5.2155060123049434E-5 6 0.0 0.0 0.0 0.0 5.2155060123049434E-5 7 0.0 0.0 0.0 0.0 5.2155060123049434E-5 8 0.0 0.0 0.0 0.0 5.2155060123049434E-5 9 0.0 0.0 0.0 0.0 5.2155060123049434E-5 10-11 0.0 0.0 0.0 5.2155060123049434E-5 5.2155060123049434E-5 12-13 0.0 0.0 0.0 7.823259018457415E-5 2.3469777055372244E-4 14-15 0.0 0.0 0.0 7.823259018457415E-5 2.8685283067677187E-4 16-17 0.0 0.0 0.0 9.12713552153365E-5 3.129303607382966E-4 18-19 0.0 0.0 0.0 1.82542710430673E-4 3.129303607382966E-4 20-21 2.6077530061524717E-5 0.0 0.0 2.3469777055372244E-4 3.129303607382966E-4 22-23 2.6077530061524717E-5 0.0 0.0 3.520466558305837E-4 3.3900789079982133E-4 24-25 2.6077530061524717E-5 0.0 0.0 5.08511836199732E-4 3.3900789079982133E-4 26-27 2.6077530061524717E-5 0.0 0.0 6.519382515381179E-4 4.563567760766825E-4 28-29 3.9116295092287075E-5 0.0 0.0 0.0010300624374302264 4.693955411074449E-4 30-31 5.2155060123049434E-5 0.0 0.0 0.002659908066275521 4.693955411074449E-4 32-33 5.2155060123049434E-5 0.0 0.0 0.0067149639908426146 4.693955411074449E-4 34-35 5.2155060123049434E-5 0.0 0.0 0.011878314943024508 4.954730711689697E-4 36-37 5.2155060123049434E-5 0.0 0.0 0.02107064428971197 4.954730711689697E-4 38-39 5.2155060123049434E-5 0.0 0.0 0.037577720818657116 5.08511836199732E-4 40-41 5.2155060123049434E-5 0.0 0.0 0.05798338809180021 5.47628131292019E-4 42-43 5.2155060123049434E-5 0.0 0.0 0.07954950545268115 5.737056613535437E-4 44-45 5.2155060123049434E-5 0.0 0.0 0.10389287976511447 5.737056613535437E-4 46-47 5.2155060123049434E-5 0.0 0.0 0.13299540331377607 5.737056613535437E-4 48-49 5.2155060123049434E-5 0.0 0.0 0.16693530868885048 5.737056613535437E-4 50-51 5.2155060123049434E-5 0.0 0.0 0.2039262850811233 6.258607214765932E-4 52-53 5.2155060123049434E-5 0.0 0.0 0.24286003746297968 6.519382515381179E-4 54-55 7.823259018457415E-5 2.6077530061524717E-5 0.0 0.2824196505663127 6.780157815996427E-4 56-57 7.823259018457415E-5 2.6077530061524717E-5 0.0 0.3309238564807486 7.040933116611674E-4 58-59 9.12713552153365E-5 2.6077530061524717E-5 0.0 0.3858561735553505 7.040933116611674E-4 60-61 1.0431012024609887E-4 2.6077530061524717E-5 0.0 0.4468515163692568 7.040933116611674E-4 62-63 1.0431012024609887E-4 2.6077530061524717E-5 0.0 0.5092420070414547 7.30170841722692E-4 64-65 1.0431012024609887E-4 2.6077530061524717E-5 0.0 0.5769653526112344 7.30170841722692E-4 66-67 1.0431012024609887E-4 2.6077530061524717E-5 0.0 0.6574536491461304 7.432096067534544E-4 68-69 1.0431012024609887E-4 2.6077530061524717E-5 0.0 0.7492856712577898 7.562483717842167E-4 70-71 1.0431012024609887E-4 2.6077530061524717E-5 0.0 0.8506751081369979 7.823259018457415E-4 72-73 1.3038765030762357E-4 2.6077530061524717E-5 0.0 0.9584274623512179 8.214421969380285E-4 74-75 1.3038765030762357E-4 2.6077530061524717E-5 0.0 1.0706129966758973 8.344809619687909E-4 76-77 1.3038765030762357E-4 2.6077530061524717E-5 0.0 1.1965674668730615 8.344809619687909E-4 78-79 1.3038765030762357E-4 2.6077530061524717E-5 2.6077530061524717E-5 1.343931589250738 8.475197269995534E-4 80-81 1.564651803691483E-4 2.6077530061524717E-5 2.6077530061524717E-5 1.5082069898733128 8.73597257061078E-4 82-83 1.564651803691483E-4 2.6077530061524717E-5 2.6077530061524717E-5 1.6826917435149746 9.127135521533651E-4 84-85 1.564651803691483E-4 3.9116295092287075E-5 2.6077530061524717E-5 1.8623268093437875 9.127135521533651E-4 86-87 1.564651803691483E-4 5.2155060123049434E-5 2.6077530061524717E-5 2.0558090436352705 9.648686122764145E-4 88-89 1.564651803691483E-4 5.2155060123049434E-5 2.6077530061524717E-5 2.2731913342281405 0.001017023672399464 90-91 1.82542710430673E-4 5.2155060123049434E-5 2.6077530061524717E-5 2.52272721938687 0.001017023672399464 92-93 1.82542710430673E-4 5.2155060123049434E-5 2.6077530061524717E-5 2.78627977695367 0.001095256262584038 94-95 2.0862024049219774E-4 5.2155060123049434E-5 2.6077530061524717E-5 3.0561430967953584 0.001134372557676325 96-97 2.0862024049219774E-4 5.2155060123049434E-5 2.6077530061524717E-5 3.3368546691426415 0.0012647602079839488 98-99 2.0862024049219774E-4 5.2155060123049434E-5 2.6077530061524717E-5 3.64144022026125 0.00138210909326081 100-101 2.216590055229601E-4 5.2155060123049434E-5 2.6077530061524717E-5 3.992482891184465 0.00138210909326081 102-103 2.3469777055372244E-4 5.2155060123049434E-5 2.6077530061524717E-5 4.371141666442835 0.0014081866233223348 104-105 2.3469777055372244E-4 5.2155060123049434E-5 2.6077530061524717E-5 4.7521604581717725 0.0014342641533838594 106-107 2.6077530061524714E-4 5.2155060123049434E-5 2.6077530061524717E-5 5.139568244765783 0.0014342641533838594 108-109 2.6077530061524714E-4 5.2155060123049434E-5 2.6077530061524717E-5 5.5524537782299035 0.0014733804484761465 110-111 2.6077530061524714E-4 5.2155060123049434E-5 2.6077530061524717E-5 6.006985127202279 0.0015385742736299583 112-113 2.6077530061524714E-4 5.2155060123049434E-5 2.6077530061524717E-5 6.488832688914103 0.0015385742736299583 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4580 0.0 59.783768 1 GTATCAA 8290 0.0 47.78038 1 ATCAACG 9335 0.0 39.764286 3 TCAACGC 9475 0.0 38.799435 4 CAACGCA 9535 0.0 38.37266 5 TATCAAC 10130 0.0 38.290497 2 AACGCAG 9995 0.0 36.428066 6 ACGCAGA 12165 0.0 29.636576 7 CGCAGAG 12370 0.0 29.56671 8 GTACATG 9965 0.0 26.878502 1 TACATGG 9875 0.0 25.824154 2 ACATGGG 9705 0.0 24.557486 3 GCAGAGT 14875 0.0 23.588081 9 GAGTACT 8945 0.0 23.468857 12-13 AGAGTAC 12555 0.0 22.594316 10-11 CAGAGTA 13625 0.0 22.173016 10-11 GTACTTT 10075 0.0 20.984184 14-15 TGGTATC 2570 0.0 20.633663 2 AGTACTT 10295 0.0 19.322681 12-13 GTGGTAT 3170 0.0 18.441782 1 >>END_MODULE