FastQCFastQC Report
Thu 26 May 2016
SRR1364477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144077
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49920.23282745908845626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41050.1914576761935322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33820.15773687232314884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105400.048.342131
GGTATCA61950.045.9281771
ATCAACG123350.038.830863
CAACGCA126000.038.4975785
TCAACGC124950.038.4395524
TATCAAC133600.037.9894372
GTACATG136800.037.3330761
AACGCAG127900.037.0662426
TACATGG136600.035.7176972
ACGCAGA144100.032.23947
CGCAGAG142850.031.6474028
ACATGGG144250.031.5548323
ACGCATA3150.030.2542238
GCAGAGT144650.029.5241439
CATGGGA100100.028.9322154
GAGTACT61300.027.36289212-13
AGTACTT64900.026.6240912-13
GTCGGGA10850.025.2259982
ATGGGAG35500.024.6756425
ATGGGAT36350.024.4265065
Produced by FastQC (version 0.11.5)