FastQCFastQC Report
Thu 26 May 2016
SRR1364477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144077
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114850.5356617322978606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103210.48137263727002344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66070.30815124643378017No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.11930541673643251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79150.057.7639051
GTATCAA137250.051.6198841
TATCAAC185600.038.0601462
ATCAACG181150.036.8232273
TCAACGC182850.036.187464
CAACGCA183750.035.942165
AACGCAG185800.034.9051366
GTACATG129000.032.9897081
TACATGG129100.031.8605272
CGCAGAG217550.030.0143478
ACGCAGA213400.029.888837
ACCGCAG6000.028.7577746
GCAGAGT222950.028.3538119
ACATGGG133100.028.2158153
CACCGCA5750.027.938595
AGTACTT142650.027.80672612-13
GAGTACT139700.027.07425512-13
CATGGGG43300.026.2951454
ATCACCG6600.026.1929343
AGAGTAC214200.025.51477810-11
Produced by FastQC (version 0.11.5)