##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364477_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2144077 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.83377509296541 31.0 28.0 31.0 25.0 34.0 2 29.02207103569508 31.0 28.0 33.0 25.0 34.0 3 29.09890782840355 31.0 28.0 34.0 25.0 34.0 4 32.48957476807036 35.0 32.0 37.0 26.0 37.0 5 31.61077937033045 35.0 32.0 37.0 19.0 37.0 6 31.633057021739425 35.0 32.0 37.0 19.0 37.0 7 31.07852376570431 35.0 31.0 37.0 16.0 37.0 8 31.170003222832015 35.0 31.0 37.0 17.0 37.0 9 31.679584735063152 37.0 30.0 39.0 10.0 39.0 10-11 31.89572925785781 37.0 30.5 39.0 11.5 39.0 12-13 31.63684466556005 37.0 30.0 39.0 10.5 39.0 14-15 32.53224814220758 37.0 31.0 40.0 10.5 41.0 16-17 32.63135559030762 37.5 31.0 40.0 10.0 41.0 18-19 32.558647613868345 38.0 31.0 40.0 10.0 41.0 20-21 32.473341209294254 38.0 30.5 40.0 10.0 41.0 22-23 32.441405089462734 37.0 30.5 40.0 10.0 41.0 24-25 32.24093327804925 37.0 30.0 40.0 9.5 41.0 26-27 32.30050226740924 37.0 30.0 40.0 9.5 41.0 28-29 32.154446645339696 37.0 30.0 40.0 8.0 41.0 30-31 31.556398161073506 37.0 30.0 40.0 4.5 41.0 32-33 31.392936914112695 36.0 30.0 40.0 2.0 41.0 34-35 31.124180241661097 36.0 30.0 40.0 2.0 41.0 36-37 31.035994509525544 36.0 30.0 40.0 2.0 41.0 38-39 30.74944649842333 36.0 29.0 40.0 2.0 41.0 40-41 30.412886757331943 35.0 27.5 40.0 2.0 41.0 42-43 30.220409295002 35.0 27.5 40.0 2.0 41.0 44-45 30.037129496748484 35.0 27.0 40.0 2.0 41.0 46-47 29.72706577235799 35.0 26.5 40.0 2.0 41.0 48-49 29.326711680597292 35.0 25.5 40.0 2.0 41.0 50-51 28.94497259193583 35.0 24.0 40.0 2.0 41.0 52-53 28.840345519307377 35.0 24.0 40.0 2.0 41.0 54-55 28.371649432366468 35.0 23.0 39.0 2.0 41.0 56-57 28.374646059819682 35.0 23.0 39.0 2.0 41.0 58-59 28.126953696159234 34.0 22.5 39.0 2.0 41.0 60-61 27.850668609382964 34.0 22.0 39.0 2.0 41.0 62-63 27.589959922148317 34.0 22.0 38.5 2.0 40.0 64-65 27.130623806887534 34.0 20.0 38.0 2.0 40.0 66-67 26.755972150253932 33.0 18.0 38.0 2.0 40.0 68-69 26.567704891195604 33.0 18.0 37.0 2.0 40.0 70-71 26.64294052872168 33.0 20.0 37.0 2.0 40.0 72-73 26.430275591781452 33.0 18.0 36.5 2.0 39.0 74-75 26.06817898797478 33.0 16.0 36.0 2.0 39.0 76-77 25.986556919364368 33.0 18.0 36.0 2.0 39.0 78-79 25.594928027304988 33.0 13.0 35.0 2.0 37.5 80-81 25.366838970801886 33.0 12.5 35.0 2.0 37.0 82-83 25.223185081505935 33.0 11.5 35.0 2.0 37.0 84-85 24.791357073463313 32.0 7.0 35.0 2.0 36.0 86-87 24.652024157714486 32.0 7.0 35.0 2.0 36.0 88-89 24.595904904534677 32.0 7.0 35.0 2.0 36.0 90-91 24.426526659257107 32.0 7.0 35.0 2.0 35.0 92-93 24.433788525318818 32.0 7.0 35.0 2.0 35.0 94-95 24.43155726216922 32.0 6.0 35.0 2.0 35.0 96-97 24.30627841257567 32.0 2.0 35.0 2.0 35.0 98-99 24.16031233952885 32.0 2.0 35.0 2.0 35.0 100-101 24.060632850406023 32.0 2.0 35.0 2.0 35.0 102-103 22.96080131450503 30.0 2.0 34.0 2.0 35.0 104-105 23.85880637682322 31.5 2.0 34.5 2.0 35.0 106-107 23.943724036030424 32.0 2.0 35.0 2.0 35.0 108-109 24.006414181953353 32.0 2.0 35.0 2.0 35.0 110-111 23.92758958750082 32.0 2.0 35.0 2.0 35.0 112-113 23.861072853260403 32.0 2.0 35.0 2.0 35.0 114-115 23.79697277663069 32.0 2.0 35.0 2.0 35.0 116-117 23.701097255369092 32.0 2.0 35.0 2.0 35.0 118-119 23.57160167288768 32.0 2.0 35.0 2.0 35.0 120-121 23.395485563251693 32.0 2.0 35.0 2.0 35.0 122-123 23.103625242936705 32.0 2.0 35.0 2.0 35.0 124-125 22.345753440757957 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0974360044513283 1101 2 1.0289451797096163 1101 3 1.0653674942725395 1101 4 1.0911835919872672 1101 5 1.2908564373504419 1101 6 1.2484352070934435 1101 7 1.717531615325207 1101 8 1.502642597873539 1101 9 2.208609748088609 1101 10-11 1.769272767612339 1101 12-13 1.8971978329871888 1101 14-15 1.9231233511936665 1101 16-17 1.8788830625022754 1101 18-19 1.8598372246280306 1101 20-21 2.158739689030817 1101 22-23 2.2571775829607645 1101 24-25 2.3824576672304687 1101 26-27 2.0640222679526232 1101 28-29 2.2305129335918608 1101 30-31 2.6311601188061786 1101 32-33 2.4509289545930493 1101 34-35 2.470207238225438 1101 36-37 2.3123887123226865 1101 38-39 2.4835185180024695 1101 40-41 2.616728139869121 1101 42-43 2.386513171793773 1101 44-45 2.3102000923507156 1101 46-47 2.1481045877988407 1101 48-49 2.1971552566223984 1101 50-51 2.327107827794645 1101 52-53 2.2161262358987486 1101 54-55 2.482070566271492 1101 56-57 2.246771848384281 1101 58-59 2.435423741273418 1101 60-61 2.5731665979603306 1101 62-63 2.554783623451577 1101 64-65 2.5841689795501956 1101 66-67 2.8529887054403567 1101 68-69 2.8095200902296718 1101 70-71 2.4516075686720242 1101 72-73 2.5961995079250393 1101 74-75 2.6059885158692815 1101 76-77 2.3847480529483107 1101 78-79 2.4032624602731083 1101 80-81 2.4608006553952 1101 82-83 2.3498236124669063 1101 84-85 2.531165395515959 1101 86-87 2.3054671297589024 1101 88-89 2.2248670942379576 1101 90-91 2.2233836246564422 1101 92-93 2.1550723434702626 1101 94-95 2.085900657010601 1101 96-97 1.8955299003925283 1101 98-99 2.0131869712376407 1101 100-101 2.118603623281583 1101 102-103 1.976451235014192 1101 104-105 2.020814739364493 1101 106-107 2.213023103011551 1101 108-109 2.1674793021274894 1101 110-111 2.2335677979984254 1101 112-113 2.142709147739051 1101 114-115 2.1891535291718647 1101 116-117 2.206589178888528 1101 118-119 2.074272696520268 1101 120-121 1.9130237897579647 1101 122-123 1.9357461393956577 1101 124-125 1.7635246745380826 1102 1 0.7629953190805985 1102 2 -1.3803048287417994 1102 3 0.29099444993920187 1102 4 0.7712553788615324 1102 5 1.1672991681492917 1102 6 0.730190552938339 1102 7 1.5649466154533727 1102 8 1.1616328414117376 1102 9 1.9835621322605768 1102 10-11 1.7893941930818222 1102 12-13 1.8907392803969145 1102 14-15 1.90152859337406 1102 16-17 1.8541028642970971 1102 18-19 1.834791782020936 1102 20-21 2.12624455648848 1102 22-23 1.9504457431936473 1102 24-25 2.256222321147817 1102 26-27 1.9459522887523946 1102 28-29 1.934817712783321 1102 30-31 2.1955493118428038 1102 32-33 1.922965916437608 1102 34-35 2.126830064029331 1102 36-37 1.650552756698005 1102 38-39 1.5880688340110893 1102 40-41 1.8463316254926134 1102 42-43 1.6929440111734237 1102 44-45 1.658462741518182 1102 46-47 1.285580687012132 1102 48-49 1.3164001682206 1102 50-51 1.3536136934479863 1102 52-53 1.25079412501767 1102 54-55 1.5640713932099217 1102 56-57 1.1927839322356917 1102 58-59 1.1529751379486513 1102 60-61 1.2767999076049392 1102 62-63 1.57506971219998 1102 64-65 1.6349786065103231 1102 66-67 1.7646837731946974 1102 68-69 1.855369201085118 1102 70-71 1.1373638142675375 1102 72-73 1.5006595145301453 1102 74-75 1.7351481443339445 1102 76-77 1.640150178692732 1102 78-79 1.4409110983694084 1102 80-81 1.5511975449773736 1102 82-83 1.2167363050802251 1102 84-85 1.312348701315635 1102 86-87 1.2725782170120539 1102 88-89 1.4433617059502417 1102 90-91 1.3097749151298999 1102 92-93 1.3187863706269773 1102 94-95 1.2697453969057726 1102 96-97 1.3481044613740352 1102 98-99 1.2408957611176348 1102 100-101 1.076857349241024 1102 102-103 0.7969035287281621 1102 104-105 1.180193747981967 1102 106-107 1.4305577826026266 1102 108-109 1.2299810434904224 1102 110-111 1.2365757586739008 1102 112-113 1.2812061964307162 1102 114-115 1.4371099585067064 1102 116-117 1.1334527715682299 1102 118-119 0.7511863475797398 1102 120-121 1.1505151386669112 1102 122-123 1.4857758215072998 1102 124-125 1.562969630195088 1103 1 0.6414510513112681 1103 2 0.6648178777090017 1103 3 0.6010074188536692 1103 4 0.6641813785456137 1103 5 0.8031721672679666 1103 6 0.8811077142739947 1103 7 1.194164649699573 1103 8 0.9725341477512259 1103 9 1.6207526904184455 1103 10-11 1.318930120739342 1103 12-13 1.3112214553362804 1103 14-15 1.147219677079292 1103 16-17 1.1777282110328784 1103 18-19 1.233417885338234 1103 20-21 1.3679363687263546 1103 22-23 1.2951646750038748 1103 24-25 1.5420826244595105 1103 26-27 1.220561948047127 1103 28-29 1.224294106701734 1103 30-31 1.8599297490805 1103 32-33 1.6645332617886588 1103 34-35 1.7574203710759484 1103 36-37 1.685891018760131 1103 38-39 1.685985337674417 1103 40-41 1.9154912959330836 1103 42-43 1.971545837450499 1103 44-45 1.857815975036882 1103 46-47 1.5243368706820775 1103 48-49 1.576982252517805 1103 50-51 1.7981298798637653 1103 52-53 1.6358617338805246 1103 54-55 1.9385002664275284 1103 56-57 1.7227988129024858 1103 58-59 1.9289409234869446 1103 60-61 1.9271521239828253 1103 62-63 2.0346187121099284 1103 64-65 2.041315936316387 1103 66-67 2.1944947828213692 1103 68-69 2.2189829259905984 1103 70-71 2.0587561936832053 1103 72-73 2.175985010663087 1103 74-75 2.188639140644778 1103 76-77 2.00556936545248 1103 78-79 2.121306428822077 1103 80-81 2.012153140654302 1103 82-83 1.9457216997352873 1103 84-85 2.1326266361507145 1103 86-87 2.0723665402650155 1103 88-89 1.903511819105315 1103 90-91 1.972511079283727 1103 92-93 1.7681093408848376 1103 94-95 1.6804057597106024 1103 96-97 1.6204768537512386 1103 98-99 1.8913813941881479 1103 100-101 1.874373621279691 1103 102-103 1.6999477913305938 1103 104-105 1.7704403603420715 1103 106-107 1.6249757056867011 1103 108-109 1.6061950800336788 1103 110-111 1.6646634232211568 1103 112-113 1.6222546006711163 1103 114-115 1.51825434157168 1103 116-117 1.5590256329521601 1103 118-119 1.6383273588745801 1103 120-121 1.540537249300538 1103 122-123 1.6378020206336537 1103 124-125 1.4683302317106133 1104 1 0.6214538276323331 1104 2 0.5559121267982832 1104 3 0.5409964678094639 1104 4 0.6132933764591684 1104 5 0.8681363274869298 1104 6 0.9171231186493642 1104 7 1.197227366512017 1104 8 1.0119666705296737 1104 9 1.5450926831831886 1104 10-11 0.9537115695863285 1104 12-13 1.2378000322333662 1104 14-15 1.2798203818161369 1104 16-17 1.0718024481755677 1104 18-19 1.069644914085778 1104 20-21 1.313981793825981 1104 22-23 1.3787363209135748 1104 24-25 1.5903962197391976 1104 26-27 1.460937543624084 1104 28-29 1.4208814752821937 1104 30-31 1.8501393613209913 1104 32-33 1.6599234120184683 1104 34-35 1.5637462116141556 1104 36-37 1.5228929285324995 1104 38-39 1.6464082148404344 1104 40-41 1.8028508323155101 1104 42-43 1.6492235500423718 1104 44-45 1.4836136415250039 1104 46-47 1.437110085914263 1104 48-49 1.1970453437129471 1104 50-51 1.5177659714767522 1104 52-53 1.3905802527833444 1104 54-55 1.6973319120006956 1104 56-57 1.5134063644957862 1104 58-59 1.611204122930559 1104 60-61 1.7229247895595279 1104 62-63 1.747234953027288 1104 64-65 1.910859168402805 1104 66-67 1.8714775387827451 1104 68-69 2.0227455771809097 1104 70-71 1.768901438742617 1104 72-73 1.8045838869541484 1104 74-75 1.9305354029874913 1104 76-77 1.7994858921346086 1104 78-79 1.8530267032572034 1104 80-81 1.8185622357699422 1104 82-83 1.8100221719903047 1104 84-85 2.0379849358083 1104 86-87 1.914159882422748 1104 88-89 1.796300605572572 1104 90-91 1.8040905746102567 1104 92-93 1.6495013644302823 1104 94-95 1.5890442897187071 1104 96-97 1.6660984341790162 1104 98-99 1.6797624554719306 1104 100-101 1.625166905947946 1104 102-103 1.6319724445007857 1104 104-105 1.5427510096366355 1104 106-107 1.6499417378191126 1104 108-109 1.6508293488096015 1104 110-111 1.7280276933712173 1104 112-113 1.6222838712278111 1104 114-115 1.5500970265265437 1104 116-117 1.5192870838743744 1104 118-119 1.5467815019027888 1104 120-121 1.4592637802733428 1104 122-123 1.5569763735966013 1104 124-125 1.457417221701835 1105 1 0.5408243339658902 1105 2 0.5170998644818283 1105 3 0.4691114588562648 1105 4 0.5622428608479098 1105 5 0.8222042426952427 1105 6 0.9235861250441033 1105 7 1.2620426476289452 1105 8 1.1823974986109036 1105 9 1.7223914419355602 1105 10-11 1.4515458852474517 1105 12-13 1.6179324718192305 1105 14-15 1.5571881913735268 1105 16-17 1.4569138958911196 1105 18-19 1.7221254587466106 1105 20-21 1.8792704914469383 1105 22-23 1.8780576050528452 1105 24-25 1.9992278336590203 1105 26-27 1.7426885897597373 1105 28-29 1.7920324337477993 1105 30-31 2.111929201099713 1105 32-33 1.8744027221346151 1105 34-35 2.0493782268585328 1105 36-37 1.8541737411686228 1105 38-39 2.0283389633022253 1105 40-41 2.153969964282698 1105 42-43 2.111326379352114 1105 44-45 1.892858070735162 1105 46-47 1.7690792786865899 1105 48-49 1.9801111746296165 1105 50-51 1.9821047062830033 1105 52-53 2.0287730084424425 1105 54-55 2.3523791641013894 1105 56-57 2.062149113185967 1105 58-59 2.045758270004164 1105 60-61 1.77651328577344 1105 62-63 1.724662131381585 1105 64-65 2.1680133478207004 1105 66-67 2.2734361239044425 1105 68-69 2.29690772882811 1105 70-71 2.273934753007474 1105 72-73 2.258141413154977 1105 74-75 2.2995322476458746 1105 76-77 2.0387358042694963 1105 78-79 2.2186680494038917 1105 80-81 2.1614104768540727 1105 82-83 2.085346962576981 1105 84-85 2.343909260807422 1105 86-87 2.2312079658284603 1105 88-89 2.121430594510965 1105 90-91 2.1403728714662975 1105 92-93 2.0132191207720886 1105 94-95 1.9627544192625557 1105 96-97 1.8893027674628158 1105 98-99 1.963439573978647 1105 100-101 2.0228218296037177 1105 102-103 1.8837600251442872 1105 104-105 1.4711470183085957 1105 106-107 1.738894123263755 1105 108-109 1.7718361854422042 1105 110-111 1.9000276806037348 1105 112-113 1.8012217442998768 1105 114-115 1.835730495845393 1105 116-117 1.836574956574058 1105 118-119 1.8526730658946562 1105 120-121 1.971061821386769 1105 122-123 2.0260215218997892 1105 124-125 1.820860388491628 1106 1 0.5721353300379732 1106 2 0.5621164762628226 1106 3 0.5400300013257535 1106 4 0.4687022483257195 1106 5 0.6156900625014856 1106 6 0.8072130009022587 1106 7 1.0137846336962468 1106 8 0.6311686110482313 1106 9 1.3345988057614058 1106 10-11 1.0314041660877002 1106 12-13 1.0039521577115096 1106 14-15 1.176011007630592 1106 16-17 1.0807356953533187 1106 18-19 1.1532665345412525 1106 20-21 1.0500399276113868 1106 22-23 0.9992026710037436 1106 24-25 0.8267077573830264 1106 26-27 1.0055945585005333 1106 28-29 1.0004280607065184 1106 30-31 1.4696151920037082 1106 32-33 1.2141876343861462 1106 34-35 1.2025397152195758 1106 36-37 1.1745303019567075 1106 38-39 1.3980442486081301 1106 40-41 1.6088578260184256 1106 42-43 1.5750943528724228 1106 44-45 1.5277255292889294 1106 46-47 1.4362052958245215 1106 48-49 1.4788661874460125 1106 50-51 1.746452558313809 1106 52-53 1.3562737372856049 1106 54-55 1.845854236071613 1106 56-57 1.6685247135796537 1106 58-59 1.574684999420839 1106 60-61 1.8061595624494728 1106 62-63 1.947396425132336 1106 64-65 1.9468613497811624 1106 66-67 1.9303544855907298 1106 68-69 1.9053032406946855 1106 70-71 1.7952254282327473 1106 72-73 1.636820920209992 1106 74-75 1.9018149941730371 1106 76-77 1.5088810337397796 1106 78-79 1.4796428161585844 1106 80-81 1.701408229901201 1106 82-83 1.573576842676637 1106 84-85 1.8093706382284864 1106 86-87 1.8555583481158848 1106 88-89 1.602807578223743 1106 90-91 1.8075727246441282 1106 92-93 1.6897333372641334 1106 94-95 1.5023433295851447 1106 96-97 1.441529725284287 1106 98-99 1.4925584630171542 1106 100-101 1.2392379185299305 1106 102-103 1.0855208553375988 1106 104-105 1.3399174012387611 1106 106-107 1.3288089347113896 1106 108-109 1.25400708364878 1106 110-111 1.3285589976642775 1106 112-113 1.0694986209503945 1106 114-115 1.1095021372639415 1106 116-117 1.3525217262782974 1106 118-119 1.1380483419940823 1106 120-121 1.1347553037272498 1106 122-123 1.4084189922805983 1106 124-125 1.4291785672876323 1107 1 0.27205242092854576 1107 2 0.4052080636773674 1107 3 0.11142324937347681 1107 4 0.24632508575380285 1107 5 0.3692675260508764 1107 6 0.6597692871630798 1107 7 0.7545992215423567 1107 8 0.8050482316634913 1107 9 1.2069742058760262 1107 10-11 0.85521992685565 1107 12-13 1.152775784581884 1107 14-15 0.9143559034633029 1107 16-17 0.9257347205888564 1107 18-19 1.1355233961945927 1107 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-4.110530011416785 2216 94-95 -3.8050474973414126 2216 96-97 -4.077275786334507 2216 98-99 -3.899248708599721 2216 100-101 -4.300658269588137 2216 102-103 -4.361616358619241 2216 104-105 -4.257558070182316 2216 106-107 -4.247546692626084 2216 108-109 -4.290797250884957 2216 110-111 -3.8836349723743524 2216 112-113 -4.063428566130241 2216 114-115 -3.9119588192466104 2216 116-117 -3.812822964945333 2216 118-119 -4.010510898811599 2216 120-121 -3.9602957564361674 2216 122-123 -4.212491438434807 2216 124-125 -4.0353308681375175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 113238.0 3 37227.0 4 17609.0 5 15342.0 6 17287.0 7 20989.0 8 22947.0 9 24056.0 10 25951.0 11 28107.0 12 31601.0 13 35004.0 14 38105.0 15 29738.0 16 18507.0 17 15576.0 18 14012.0 19 12713.0 20 12443.0 21 13434.0 22 15539.0 23 20457.0 24 28003.0 25 36814.0 26 44517.0 27 49685.0 28 55004.0 29 62576.0 30 70504.0 31 78725.0 32 91595.0 33 108860.0 34 133358.0 35 169058.0 36 229649.0 37 295212.0 38 110179.0 39 456.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28945780938631 20.326825277949496 14.759477736959767 22.62423917570442 2 15.703523154295587 21.75042547525201 38.708849931881986 23.83720143857042 3 18.518458059108887 26.828187607068216 30.818109610802225 23.835244723020672 4 12.069116170359706 18.267731473239422 37.24869831274791 32.41445404365296 5 12.139536033454021 39.9575668224602 33.55089392778338 14.3520032163024 6 29.390710759713297 38.723046614106245 17.4483879272711 14.437854698909364 7 26.106135025715606 35.06472177080519 20.734155728843707 18.094987474635495 8 26.154237930820585 35.16744034845763 19.4708025877802 19.207519132941584 9 26.255866743591767 15.9040463565441 20.576453177754345 37.263633722109795 10-11 24.371158869865297 27.90202526698143 26.805998704329113 20.920817158824157 12-13 25.704819032667448 25.779770258402163 27.76593109990807 20.749479609022316 14-15 22.584081503107065 26.295442000256052 27.203833091852836 23.91664340478404 16-17 20.848794241557474 29.6855034964138 27.52615751294612 21.939544749082607 18-19 20.60989448439234 26.915365036988533 32.007776896265604 20.466963582353525 20-21 21.87200837683637 25.935426998273094 31.927686820125395 20.264877804765142 22-23 21.263000345844123 25.971634252233937 31.774119712438658 20.991245689483286 24-25 20.840899136973658 26.086408979242687 32.14132960068882 20.931362283094842 26-27 20.865624352109872 26.37650051061613 32.10874082925738 20.649134308016613 28-29 20.54494704190496 26.326972422891444 32.30855410186489 20.819526433338705 30-31 20.78632164506667 26.018057338907184 32.24234967414609 20.95327134188005 32-33 20.543171297645838 26.175623520632442 32.015787693993914 21.26541748772781 34-35 20.98020216436436 26.184401231782413 31.938354910513876 20.89704169333935 36-37 21.201022831816925 25.901525036175737 31.69673246511563 21.200719666891708 38-39 21.015869151385534 26.23676841962151 31.600503809235303 21.14685861975765 40-41 20.85942406405103 26.216878832279523 31.63174856071982 21.291948542949633 42-43 21.120847067032265 26.31093762899027 31.117456298969664 21.450759005007797 44-45 21.312007955040215 26.370146771331537 30.67030402049953 21.64754125312872 46-47 21.375821334358175 26.677135842453055 29.95939502031648 21.987647802872292 48-49 21.563078191688078 26.995462383114038 29.22017259641328 22.2212868287846 50-51 21.551434325882816 27.225234225051555 28.758863270987632 22.464468178077997 52-53 21.857337946358193 27.298474772792357 28.560511437571652 22.2836758432778 54-55 21.659679314268793 27.034386741023024 28.50204599859747 22.803887946110713 56-57 21.883290299952613 27.376016290497706 28.207937979694712 22.532755429854966 58-59 21.848101035574057 27.063446663400303 28.283365822068383 22.805086478957257 60-61 21.937854843832568 27.0840319634043 28.514111200297375 22.464001992465757 62-63 22.018020001287276 26.97998005654687 28.531614291004644 22.470385651161205 64-65 21.8777717599417 26.900676224836417 28.497093276277713 22.72445873894417 66-67 21.92547307010194 27.258044834126515 28.14511908503421 22.671363010737334 68-69 22.09275208741636 26.94425192090812 28.340962240408054 22.622033751267466 70-71 21.74373780034188 27.210140787261285 28.66358211113262 22.382539301264217 72-73 22.09154383966993 27.03597998674495 28.362651569603003 22.509824603982114 74-75 21.663440188416068 27.362051438482244 28.220478890063493 22.75402948303819 76-77 21.63855419438988 27.23279197905446 28.457631201707624 22.67102262484804 78-79 21.756060043543883 27.32373962474651 28.207886591879642 22.712313739829966 80-81 21.322799801406063 27.259443298002612 28.4706657677547 22.94709113283663 82-83 21.432834010976777 27.515067704100492 28.081233260535733 22.970865024387003 84-85 21.40140022956265 27.084428404390327 27.973598895935176 23.540572470111847 86-87 21.35256777996987 27.0396955323287 28.0948849617783 23.512851725923127 88-89 21.5282443470845 26.793423749509397 28.177534230219138 23.500797673186963 90-91 21.2667129649279 27.063937774390656 28.32567422037257 23.34367504030887 92-93 21.43119332807532 27.056350450483645 28.3554700053031 23.156986216137934 94-95 21.190335098038254 26.948725608790546 28.271892823852042 23.589046469319157 96-97 21.50410349118575 26.911075788189333 28.025030456691795 23.55979026393312 98-99 21.0312924935281 26.813129102436413 27.927175173087342 24.22840323094815 100-101 21.222536217655268 27.056282709693008 28.10646761667516 23.61471345597656 102-103 21.47298852518082 26.892377366586718 28.08365505814367 23.550979050088795 104-105 21.310776571221353 26.702548537887594 28.268843520916388 23.71783136997466 106-107 20.84629055566866 27.179629252621584 28.090675020853983 23.88340517085577 108-109 21.164193535072094 27.27719836489043 27.648943496185886 23.90966460385159 110-111 20.957280615613225 27.12145828955957 27.860285573232446 24.060975521594756 112-113 21.183947009291657 27.098644916173605 27.788080075594408 23.929327998940337 114-115 20.81171172263644 27.291268864787316 27.659656336488386 24.23736307608786 116-117 20.823505872223805 27.28908523341279 27.637370299667406 24.250038594695994 118-119 20.893235599203003 27.155338873010304 27.595767445504954 24.35565808228174 120-121 20.689655172413794 27.32566925431944 27.587512849645616 24.397162723621147 122-123 20.892497719864846 27.242898582899787 27.28874614837308 24.575857548862288 124-125 20.82801693628603 27.109018887154008 27.255666321115434 24.807297855444528 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1060.0 1 809.5 2 841.0 3 1170.5 4 915.5 5 699.0 6 1277.0 7 2885.5 8 4709.5 9 5351.5 10 4169.0 11 2628.5 12 2147.5 13 2173.0 14 2319.5 15 2513.0 16 2774.5 17 3053.5 18 3450.5 19 3863.0 20 4374.0 21 5056.0 22 5951.5 23 7222.5 24 8717.0 25 11196.0 26 15805.5 27 20461.5 28 23199.0 29 26588.5 30 31286.0 31 36289.5 32 41795.5 33 47285.5 34 53234.5 35 57897.0 36 61086.0 37 63811.0 38 63601.0 39 62191.0 40 62250.0 41 64262.5 42 66285.0 43 68780.5 44 72701.0 45 74112.5 46 73306.5 47 73210.0 48 75956.0 49 76507.0 50 73464.0 51 70328.0 52 66404.0 53 63429.0 54 59960.0 55 55249.0 56 50806.5 57 46196.5 58 42875.5 59 39456.0 60 34802.0 61 29737.5 62 24591.0 63 18843.5 64 13648.0 65 10776.0 66 8497.0 67 6611.0 68 5324.5 69 4080.0 70 3099.5 71 2621.0 72 2279.0 73 2135.0 74 1736.5 75 1305.5 76 1018.0 77 683.5 78 457.5 79 293.5 80 204.5 81 154.5 82 102.0 83 59.0 84 42.5 85 34.5 86 26.5 87 16.0 88 10.0 89 5.5 90 4.5 91 3.0 92 0.0 93 0.5 94 1.5 95 1.0 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10825170924365123 2 1.8656046401318609E-4 3 0.0 4 0.18516126053308718 5 0.0 6 2.332005800164826E-4 7 0.03852473581872293 8 0.0 9 0.0 10-11 6.529616240461513E-4 12-13 7.462418560527443E-4 14-15 5.3636133403791E-4 16-17 0.004407490962311521 18-19 0.001585763944112082 20-21 0.0032414880622291084 22-23 0.002215405510156585 24-25 0.0051304127603626175 26-27 0.004594051426324707 28-29 0.003288128178232405 30-31 8.395220880593374E-4 32-33 9.328023200659304E-5 34-35 0.0013758834220972474 36-37 0.0013758834220972474 38-39 5.3636133403791E-4 40-41 0.001702364234120323 42-43 9.328023200659304E-4 44-45 0.0012826031900906544 46-47 2.332005800164826E-4 48-49 0.0 50-51 0.041626303532942145 52-53 1.3992034800988957E-4 54-55 0.1374251018037132 56-57 2.332005800164826E-4 58-59 2.332005800164826E-5 60-61 0.0 62-63 6.996017400494478E-4 64-65 0.06401355921452448 66-67 0.05286657148973661 68-69 0.0018189645241285645 70-71 0.0028916871922043843 72-73 0.0698202536569349 74-75 0.004594051426324707 76-77 0.001888924698133509 78-79 0.0015391238281087852 80-81 1.6324040601153783E-4 82-83 2.0988052201483434E-4 84-85 0.0 86-87 3.2648081202307566E-4 88-89 0.0013292433060939509 90-91 3.7312092802637217E-4 92-93 0.002238725568158233 94-95 0.004314210730304928 96-97 0.0022853656841615297 98-99 2.332005800164826E-5 100-101 0.0012126430160857096 102-103 0.0016090840021137302 104-105 2.0988052201483434E-4 106-107 1.166002900082413E-4 108-109 2.332005800164826E-5 110-111 1.6324040601153783E-4 112-113 1.3992034800988957E-4 114-115 1.3992034800988957E-4 116-117 0.0 118-119 0.0013525633640955994 120-121 0.0022853656841615297 122-123 7.229217980510961E-4 124-125 0.007439098502525796 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2144077.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.01427772196169 #Duplication Level Percentage of deduplicated Percentage of total 1 76.79221532321203 39.174993993830206 2 10.830732556673823 11.050439971569011 3 4.481243642693024 6.858222231843516 4 2.3787536256006625 4.8540159235406195 5 1.3968667475503764 3.563007410005412 6 0.9250776613536907 2.8315301238408006 7 0.6433554277522243 2.2974218725698403 8 0.46678006981855596 1.9049958493440384 9 0.32913424930236196 1.511149140154806 >10 1.5973984902766665 14.086682886031362 >50 0.09811455668859984 3.4591041910147835 >100 0.055898829499190165 5.2822923773971215 >500 0.003109596725613019 1.0593374948326084 >1k 0.0010365322418709995 0.7009692494281899 >5k 9.423020380645451E-5 0.317604157932523 >10k+ 1.8846040761290902E-4 1.0482331266651426 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11485 0.5356617322978606 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10321 0.48137263727002344 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6607 0.30815124643378017 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2558 0.11930541673643251 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.996017400494479E-5 0.0 12-13 0.0 0.0 0.0 1.1660029000824131E-4 0.0 14-15 0.0 0.0 0.0 1.3992034800988957E-4 0.0 16-17 0.0 0.0 0.0 1.6324040601153783E-4 0.0 18-19 0.0 0.0 0.0 2.0988052201483437E-4 0.0 20-21 0.0 0.0 0.0 3.964409860280205E-4 0.0 22-23 2.332005800164826E-5 0.0 0.0 8.162020300576892E-4 0.0 24-25 4.664011600329652E-5 0.0 0.0 0.001515803770107137 0.0 26-27 4.664011600329652E-5 0.0 0.0 0.0025185662641780122 0.0 28-29 4.664011600329652E-5 0.0 0.0 0.004640691542328004 0.0 30-31 4.664011600329652E-5 0.0 0.0 0.012522871146885117 0.0 32-33 4.664011600329652E-5 0.0 0.0 0.02385641933568617 0.0 34-35 4.664011600329652E-5 0.0 0.0 0.038408135528714686 0.0 36-37 4.664011600329652E-5 0.0 0.0 0.06270763596643218 0.0 38-39 4.664011600329652E-5 0.0 0.0 0.10442721973138092 0.0 40-41 4.664011600329652E-5 0.0 0.0 0.1575736319171373 0.0 42-43 4.664011600329652E-5 0.0 0.0 0.20631255314058217 0.0 44-45 4.664011600329652E-5 2.332005800164826E-5 0.0 0.2640763368106649 0.0 46-47 4.664011600329652E-5 9.328023200659304E-5 0.0 0.3325673471615058 0.0 48-49 4.664011600329652E-5 9.328023200659304E-5 0.0 0.41033974059700284 0.0 50-51 4.664011600329652E-5 9.328023200659304E-5 0.0 0.49771999792917887 0.0 52-53 4.664011600329652E-5 9.328023200659304E-5 0.0 0.5818354471411241 0.0 54-55 4.664011600329652E-5 1.1660029000824131E-4 0.0 0.6673967399491716 0.0 56-57 4.664011600329652E-5 1.3992034800988957E-4 0.0 0.7674631088342443 0.0 58-59 4.664011600329652E-5 1.3992034800988957E-4 0.0 0.8856724828445993 0.0 60-61 4.664011600329652E-5 1.3992034800988957E-4 0.0 1.022537903256273 0.0 62-63 4.664011600329652E-5 1.3992034800988957E-4 0.0 1.1553223135176582 0.0 64-65 4.664011600329652E-5 1.3992034800988957E-4 0.0 1.2816004275965835 0.0 66-67 4.664011600329652E-5 1.3992034800988957E-4 0.0 1.4275839906869017 0.0 68-69 4.664011600329652E-5 1.3992034800988957E-4 0.0 1.606775316371567 0.0 70-71 4.664011600329652E-5 1.3992034800988957E-4 0.0 1.817472040416459 0.0 72-73 4.664011600329652E-5 1.3992034800988957E-4 0.0 2.025860078719188 2.332005800164826E-5 74-75 4.664011600329652E-5 1.3992034800988957E-4 0.0 2.231193189423701 4.664011600329652E-5 76-77 4.664011600329652E-5 1.3992034800988957E-4 0.0 2.454389464557476 4.664011600329652E-5 78-79 4.664011600329652E-5 1.3992034800988957E-4 0.0 2.71151642408365 4.664011600329652E-5 80-81 4.664011600329652E-5 1.3992034800988957E-4 0.0 3.0001487819700507 4.664011600329652E-5 82-83 4.664011600329652E-5 1.3992034800988957E-4 0.0 3.3116347966980664 4.664011600329652E-5 84-85 4.664011600329652E-5 1.3992034800988957E-4 0.0 3.6254994573422503 4.664011600329652E-5 86-87 4.664011600329652E-5 1.3992034800988957E-4 0.0 3.947922579273039 4.664011600329652E-5 88-89 4.664011600329652E-5 1.3992034800988957E-4 0.0 4.305675589076325 4.664011600329652E-5 90-91 4.664011600329652E-5 1.3992034800988957E-4 0.0 4.714709406425236 4.664011600329652E-5 92-93 4.664011600329652E-5 1.3992034800988957E-4 0.0 5.166955291251201 4.664011600329652E-5 94-95 4.664011600329652E-5 1.3992034800988957E-4 0.0 5.614280643838818 4.664011600329652E-5 96-97 4.664011600329652E-5 1.3992034800988957E-4 0.0 6.059483871148284 4.664011600329652E-5 98-99 4.664011600329652E-5 1.3992034800988957E-4 0.0 6.534676693047871 4.664011600329652E-5 100-101 4.664011600329652E-5 1.3992034800988957E-4 0.0 7.092515800505299 4.664011600329652E-5 102-103 4.664011600329652E-5 1.3992034800988957E-4 0.0 7.67614689211255 4.664011600329652E-5 104-105 4.664011600329652E-5 1.3992034800988957E-4 0.0 8.251056282027184 4.664011600329652E-5 106-107 4.664011600329652E-5 1.3992034800988957E-4 0.0 8.833078289632322 4.664011600329652E-5 108-109 4.664011600329652E-5 1.3992034800988957E-4 0.0 9.429092332038449 4.664011600329652E-5 110-111 4.664011600329652E-5 1.3992034800988957E-4 0.0 10.109431704178535 4.664011600329652E-5 112-113 4.664011600329652E-5 1.3992034800988957E-4 0.0 10.828342452253347 4.664011600329652E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7915 0.0 57.763905 1 GTATCAA 13725 0.0 51.619884 1 TATCAAC 18560 0.0 38.060146 2 ATCAACG 18115 0.0 36.823227 3 TCAACGC 18285 0.0 36.18746 4 CAACGCA 18375 0.0 35.94216 5 AACGCAG 18580 0.0 34.905136 6 GTACATG 12900 0.0 32.989708 1 TACATGG 12910 0.0 31.860527 2 CGCAGAG 21755 0.0 30.014347 8 ACGCAGA 21340 0.0 29.88883 7 ACCGCAG 600 0.0 28.757774 6 GCAGAGT 22295 0.0 28.353811 9 ACATGGG 13310 0.0 28.215815 3 CACCGCA 575 0.0 27.93859 5 AGTACTT 14265 0.0 27.806726 12-13 GAGTACT 13970 0.0 27.074255 12-13 CATGGGG 4330 0.0 26.295145 4 ATCACCG 660 0.0 26.192934 3 AGAGTAC 21420 0.0 25.514778 10-11 >>END_MODULE