FastQCFastQC Report
Thu 26 May 2016
SRR1364476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3628873
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52760.14538949144817137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37080.10218048413377927No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152200.039.9697881
GTACATG164900.034.9422071
GGTATCA97050.034.776281
ATCAACG176350.032.256433
TACATGG173750.032.1564942
CAACGCA178800.031.688685
TCAACGC178300.031.6109054
AACGCAG175600.031.5357976
TATCAAC192450.031.1343292
ACATGGG171600.029.6124613
GAGTACT73300.027.4689412-13
ACGCAGA198000.027.367787
CGCAGAG196750.026.6956928
CATGGGA104500.025.6562614
CGCATAG5200.025.1945539
GCAGAGT198400.024.7027639
AACGCAT5650.024.2395636
AGAGTAC156050.023.99007410-11
GTACTTT86150.023.75935214-15
CATGGGG75100.021.8167424
Produced by FastQC (version 0.11.5)