##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364476_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3628873 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.028374649650182 31.0 27.0 31.0 10.0 34.0 2 27.200406021373578 31.0 27.0 31.0 10.0 34.0 3 27.14773346986792 31.0 27.0 31.0 10.0 34.0 4 29.96350299390472 35.0 28.0 37.0 10.0 37.0 5 30.65527258738457 35.0 30.0 37.0 12.0 37.0 6 30.800221997297783 35.0 31.0 37.0 13.0 37.0 7 30.58473470964677 35.0 30.0 37.0 10.0 37.0 8 30.197982128335713 35.0 30.0 37.0 10.0 37.0 9 30.84877260791436 35.0 29.0 39.0 8.0 39.0 10-11 30.990398396416737 36.0 29.5 39.0 8.0 39.0 12-13 30.874335365277318 36.5 29.5 39.0 8.0 39.0 14-15 31.593014001867797 37.0 29.5 40.0 8.0 41.0 16-17 31.893052608895378 37.0 30.0 40.0 8.0 41.0 18-19 31.87869250315456 37.0 30.0 40.0 8.0 41.0 20-21 31.66630893393073 37.0 29.5 40.0 7.0 41.0 22-23 31.717453049472937 37.0 30.0 40.0 4.5 41.0 24-25 31.742216247303226 37.0 30.0 40.0 2.0 41.0 26-27 31.869643826058393 37.0 30.0 40.0 2.0 41.0 28-29 31.81408222332388 37.0 30.0 40.0 2.0 41.0 30-31 31.46742721500587 37.0 30.0 40.0 2.0 41.0 32-33 31.46340695857915 37.0 30.0 40.0 2.0 41.0 34-35 31.160486602865408 37.0 29.5 40.0 2.0 41.0 36-37 30.920964029328115 36.0 29.0 40.0 2.0 41.0 38-39 30.778934672004226 36.0 29.0 40.0 2.0 41.0 40-41 30.803833173550025 36.0 29.0 40.0 2.0 41.0 42-43 30.65956978929822 36.0 28.5 40.0 2.0 41.0 44-45 30.297030097223022 36.0 27.5 40.0 2.0 41.0 46-47 30.034898300381414 35.5 27.5 40.0 2.0 41.0 48-49 29.766945136961255 35.0 26.5 40.0 2.0 41.0 50-51 29.745641277608776 35.0 27.0 40.0 2.0 41.0 52-53 29.556565495678683 35.0 26.5 40.0 2.0 41.0 54-55 29.433603352886696 35.0 26.5 40.0 2.0 41.0 56-57 29.00848679466049 35.0 26.0 39.0 2.0 41.0 58-59 28.77494527915416 34.5 25.0 39.0 2.0 41.0 60-61 28.363532837881074 34.0 23.5 39.0 2.0 40.0 62-63 28.32635710866707 34.0 24.5 39.0 2.0 40.0 64-65 28.14877924909469 34.0 24.0 38.0 2.0 40.0 66-67 27.88322627989461 34.0 23.5 38.0 2.0 40.0 68-69 27.70295667552984 34.0 23.5 37.5 2.0 40.0 70-71 27.54433993694461 34.0 23.5 37.0 2.0 40.0 72-73 27.52056285794515 34.0 24.5 37.0 2.0 39.5 74-75 27.247626742517582 33.5 24.0 36.0 2.0 39.0 76-77 26.94588306066374 33.0 23.0 36.0 2.0 39.0 78-79 26.618106089686798 33.0 22.5 35.5 2.0 38.5 80-81 26.61628086736571 33.0 24.0 35.0 2.0 37.0 82-83 26.406541783082517 33.0 23.0 35.0 2.0 37.0 84-85 26.214232352578886 33.0 23.0 35.0 2.0 36.0 86-87 25.9958972110625 33.0 22.5 35.0 2.0 36.0 88-89 25.763526169143972 33.0 20.5 35.0 2.0 36.0 90-91 25.589277580119226 33.0 20.0 35.0 2.0 35.5 92-93 25.478988242355136 33.0 20.0 35.0 2.0 35.0 94-95 25.40972941185872 33.0 20.0 35.0 2.0 35.0 96-97 25.269927054487717 33.0 19.0 35.0 2.0 35.0 98-99 25.1787512266205 33.0 18.5 35.0 2.0 35.0 100-101 25.123368605073807 33.0 18.0 35.0 2.0 35.0 102-103 23.958982857763278 30.5 12.5 34.0 2.0 35.0 104-105 24.734475965403036 32.0 15.0 35.0 2.0 35.0 106-107 24.817145295522877 32.5 11.0 35.0 2.0 35.0 108-109 24.76441115464774 33.0 8.5 35.0 2.0 35.0 110-111 24.655419464941318 33.0 7.0 35.0 2.0 35.0 112-113 24.641389627027454 33.0 6.0 35.0 2.0 35.0 114-115 24.59119360198056 33.0 2.0 35.0 2.0 35.0 116-117 24.382144814657334 33.0 2.0 35.0 2.0 35.0 118-119 24.234773440679795 32.0 2.0 35.0 2.0 35.0 120-121 24.086624690365298 32.5 2.0 35.0 2.0 35.0 122-123 23.81679642687964 32.0 2.0 35.0 2.0 35.0 124-125 22.82648166524428 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.0629701827394804 1101 2 1.6720130673928537 1101 3 1.9278361154882226 1101 4 2.317155941604426 1101 5 1.7625988875921443 1101 6 1.7323263379430571 1101 7 1.600296681429029 1101 8 1.999820325728951 1101 9 2.507285052723873 1101 10-11 2.5705983625927473 1101 12-13 2.3823112905184196 1101 14-15 2.6873655450434484 1101 16-17 2.3473406510131447 1101 18-19 2.291004790346193 1101 20-21 2.6296353798248724 1101 22-23 2.544568135985916 1101 24-25 2.4008614864238957 1101 26-27 2.1409978444323947 1101 28-29 2.0191419373661255 1101 30-31 2.267865122470411 1101 32-33 2.4134556900258666 1101 34-35 2.754181775355253 1101 36-37 2.750704805309983 1101 38-39 2.6665314436928007 1101 40-41 2.514085583658744 1101 42-43 2.5630059242185688 1101 44-45 2.6888291429395927 1101 46-47 2.650344200114297 1101 48-49 2.810345074004079 1101 50-51 2.6313775666046304 1101 52-53 2.5456150615253925 1101 54-55 2.6117053918313324 1101 56-57 2.8517311746079734 1101 58-59 2.793102910728301 1101 60-61 2.918762327925119 1101 62-63 2.610467469274642 1101 64-65 2.4973658135623573 1101 66-67 2.5550062953267414 1101 68-69 2.4470105285531574 1101 70-71 2.5124033515140205 1101 72-73 2.33931579192625 1101 74-75 2.3414546243022976 1101 76-77 2.522352267371776 1101 78-79 2.554298642979834 1101 80-81 2.3038679605438155 1101 82-83 2.266606337976505 1101 84-85 2.1628639973642727 1101 86-87 2.148933088373827 1101 88-89 2.241891264663362 1101 90-91 2.217299975436031 1101 92-93 2.2013065612069553 1101 94-95 2.1473740996557353 1101 96-97 2.2518426249436025 1101 98-99 2.1909039238526695 1101 100-101 2.181439881243861 1101 102-103 2.205175090944188 1101 104-105 2.164243351702627 1101 106-107 2.278940978130727 1101 108-109 2.2840798712164485 1101 110-111 2.4141382301749594 1101 112-113 2.307636035297456 1101 114-115 2.221041712426075 1101 116-117 2.368779540551621 1101 118-119 2.3395454700696234 1101 120-121 2.3185221296401437 1101 122-123 2.391265519133679 1101 124-125 2.327111320932353 1102 1 1.0823488658187337 1102 2 0.8532232881929289 1102 3 0.9455955720999789 1102 4 1.4545844508248393 1102 5 1.2821093094023261 1102 6 1.1704559201245672 1102 7 1.0648634340207082 1102 8 1.3335034503409595 1102 9 1.8105371136930515 1102 10-11 1.3863295741095527 1102 12-13 1.7020223012324784 1102 14-15 1.9607627130171323 1102 16-17 1.495595420629133 1102 18-19 1.6758917399822195 1102 20-21 1.724510108150195 1102 22-23 1.6479958053962172 1102 24-25 1.3303529801065288 1102 26-27 1.4250864884630694 1102 28-29 1.169723940028117 1102 30-31 1.067575822143695 1102 32-33 1.0124036269830832 1102 34-35 1.4842183356070926 1102 36-37 1.6688932713994085 1102 38-39 1.7210356706074172 1102 40-41 1.272909713424955 1102 42-43 1.2969960072016526 1102 44-45 1.7845257641295085 1102 46-47 1.6461655616175612 1102 48-49 1.6334297755928482 1102 50-51 1.5164197126305048 1102 52-53 1.6218900371281357 1102 54-55 1.4348659096105543 1102 56-57 1.5625658914247822 1102 58-59 1.592940683520787 1102 60-61 1.9505894462748437 1102 62-63 1.8298517899680533 1102 64-65 1.9702258205866947 1102 66-67 1.8488080949568584 1102 68-69 1.6757797272213892 1102 70-71 1.8599124008567536 1102 72-73 1.7806025352385468 1102 74-75 1.8585353964091986 1102 76-77 1.7592127707653589 1102 78-79 1.6510250035564518 1102 80-81 1.287046615888599 1102 82-83 1.1602647672464812 1102 84-85 1.2527539731678914 1102 86-87 1.3780947492279196 1102 88-89 1.2128894982109273 1102 90-91 1.0723709121742253 1102 92-93 1.1195689966821938 1102 94-95 1.3124113662293482 1102 96-97 1.410397931234531 1102 98-99 1.510495021865509 1102 100-101 1.4615517392848467 1102 102-103 1.2083425553008595 1102 104-105 1.1365966862542045 1102 106-107 1.426818157198202 1102 108-109 1.5766880518481976 1102 110-111 1.3508823848762752 1102 112-113 1.3652453240119868 1102 114-115 1.489678368841357 1102 116-117 1.698171047583088 1102 118-119 1.795431690055807 1102 120-121 1.780202747781857 1102 122-123 1.839139263792525 1102 124-125 1.7685690394741442 1103 1 1.5636777989953572 1103 2 1.3359079539863536 1103 3 1.503641698786435 1103 4 2.0448542935743355 1103 5 1.44591786030092 1103 6 1.4236538019244946 1103 7 1.4400231142853883 1103 8 1.697336731299103 1103 9 2.3426149800655196 1103 10-11 1.9077807064547727 1103 12-13 2.043186238142635 1103 14-15 2.316879318451271 1103 16-17 2.0359458442915646 1103 18-19 1.8975290685335509 1103 20-21 2.3208086236739085 1103 22-23 2.127755629108055 1103 24-25 2.103144853270795 1103 26-27 1.9086112555216097 1103 28-29 1.765599097433988 1103 30-31 2.0810665341163386 1103 32-33 2.1229764321822806 1103 34-35 1.9555010024300792 1103 36-37 2.2322181125840608 1103 38-39 2.302490544495331 1103 40-41 2.289171441383406 1103 42-43 2.2412010366935355 1103 44-45 2.174853331707123 1103 46-47 2.371781786971191 1103 48-49 2.3965464774485916 1103 50-51 2.2389456575702447 1103 52-53 2.203547524522051 1103 54-55 2.192091844011191 1103 56-57 2.329300629822715 1103 58-59 2.543676963383053 1103 60-61 2.496218536384834 1103 62-63 2.3242751455232025 1103 64-65 2.297814204121728 1103 66-67 2.233523925110042 1103 68-69 2.0140474602277187 1103 70-71 2.2382859637824204 1103 72-73 2.281753123682627 1103 74-75 2.3065028706148176 1103 76-77 2.233047647004547 1103 78-79 2.379307190193444 1103 80-81 1.9149523898769978 1103 82-83 1.9886718374808083 1103 84-85 1.887566433145718 1103 86-87 1.9388057480500365 1103 88-89 2.0752810999852116 1103 90-91 2.092768021832498 1103 92-93 2.059124198371528 1103 94-95 1.8174958440486968 1103 96-97 1.9980613474359892 1103 98-99 1.9406682251802359 1103 100-101 1.7807317263880584 1103 102-103 1.8842706292680802 1103 104-105 2.0191482037412705 1103 106-107 1.8125211075391547 1103 108-109 2.076152879905411 1103 110-111 2.227918682645413 1103 112-113 2.095150468857419 1103 114-115 2.006181471150448 1103 116-117 2.1587459349764266 1103 118-119 2.289374443171333 1103 120-121 2.2835936489779023 1103 122-123 2.288721127176885 1103 124-125 2.269516229585193 1104 1 1.491288443214252 1104 2 1.3162656723103154 1104 3 1.3547857479503023 1104 4 1.6896662212732032 1104 5 1.2964652263156111 1104 6 1.0915139556205844 1104 7 1.1089083653458047 1104 8 1.5406249905915388 1104 9 2.0117835869559997 1104 10-11 1.8370246157305559 1104 12-13 1.3986909427760246 1104 14-15 1.6521779423856628 1104 16-17 1.4454199346033043 1104 18-19 0.7985936208127811 1104 20-21 -3.6034380906374643 1104 22-23 -0.42524633847643756 1104 24-25 1.1108916229826384 1104 26-27 1.1986647928107779 1104 28-29 0.6950324186730761 1104 30-31 1.5197851916882144 1104 32-33 1.3146696544031862 1104 34-35 1.538038619893495 1104 36-37 1.6531377803309333 1104 38-39 1.5137869617071438 1104 40-41 1.5938144083592043 1104 42-43 1.570161792363809 1104 44-45 1.7171519764977994 1104 46-47 1.5949364404281852 1104 48-49 1.7351630176600246 1104 50-51 1.591648812728959 1104 52-53 1.627735337160825 1104 54-55 1.5575220614958454 1104 56-57 1.8544079242825156 1104 58-59 1.8284369863786978 1104 60-61 1.8804672723380413 1104 62-63 1.4849765954762368 1104 64-65 1.5635651648317044 1104 66-67 1.5525850949271423 1104 68-69 1.4182507487791796 1104 70-71 1.6642856027361468 1104 72-73 1.4803620029037745 1104 74-75 1.506106920985392 1104 76-77 1.452509453835006 1104 78-79 1.4585017340423185 1104 80-81 1.2902741495070416 1104 82-83 1.187524155820764 1104 84-85 1.2326749397454968 1104 86-87 1.132318493191839 1104 88-89 1.2874431137215687 1104 90-91 1.321862764848408 1104 92-93 0.9696307355517035 1104 94-95 1.1131033880143484 1104 96-97 1.2097662582357955 1104 98-99 1.1937902477444489 1104 100-101 0.7929122089604235 1104 102-103 1.149424212625206 1104 104-105 1.3254694533730778 1104 106-107 1.4060986309385726 1104 108-109 1.4232143776281028 1104 110-111 1.465805983025259 1104 112-113 1.4209603396911668 1104 114-115 1.300121009692738 1104 116-117 1.3827301467324489 1104 118-119 1.3266936555568165 1104 120-121 1.332291659423376 1104 122-123 1.2466835331503958 1104 124-125 1.3652724810326475 1105 1 1.5847206772955467 1105 2 1.2995132409860943 1105 3 1.3907790060200789 1105 4 2.011418229420542 1105 5 1.5388243943243616 1105 6 1.5243114025222084 1105 7 1.5041422765189694 1105 8 1.7124857133047975 1105 9 2.2262896715352767 1105 10-11 1.9448548664297647 1105 12-13 2.063392311039845 1105 14-15 2.262623632641798 1105 16-17 2.0969916177920815 1105 18-19 1.7308283851396808 1105 20-21 1.9944729728819333 1105 22-23 1.8317426979030493 1105 24-25 1.8340346748614529 1105 26-27 1.737873830897545 1105 28-29 1.2826727303449807 1105 30-31 1.7053470552304901 1105 32-33 1.7168916912452055 1105 34-35 1.6184905861602878 1105 36-37 1.9283520037612867 1105 38-39 1.7530473608388384 1105 40-41 1.9031473134156762 1105 42-43 2.1325157978574616 1105 44-45 2.272770032101416 1105 46-47 2.50463157153834 1105 48-49 2.172235583205996 1105 50-51 2.244459836504941 1105 52-53 2.1867573863156444 1105 54-55 2.19079656595693 1105 56-57 2.5258806539021172 1105 58-59 2.2857621326661537 1105 60-61 2.569833447475954 1105 62-63 2.4654447814996914 1105 64-65 2.1745792751396458 1105 66-67 2.3834447711731492 1105 68-69 1.9856312699169578 1105 70-71 2.0427235657945637 1105 72-73 2.0598729622135856 1105 74-75 2.2597231708319363 1105 76-77 2.200819478439982 1105 78-79 2.0186636438244037 1105 80-81 1.932718098605843 1105 82-83 2.0517962120487674 1105 84-85 1.9806711182821815 1105 86-87 1.9728572781119773 1105 88-89 2.047842336484674 1105 90-91 2.105407865729042 1105 92-93 2.018464861992584 1105 94-95 1.9353873877875216 1105 96-97 2.097802322256083 1105 98-99 1.870508109099557 1105 100-101 1.9729119223901392 1105 102-103 1.9865131648914591 1105 104-105 2.0543080441175654 1105 106-107 2.1720135120355835 1105 108-109 2.210658670975537 1105 110-111 2.141742424286182 1105 112-113 2.0966788175366737 1105 114-115 2.0345088791726056 1105 116-117 2.17990092088629 1105 118-119 2.20169661536643 1105 120-121 2.146774208203766 1105 122-123 2.2047421657948547 1105 124-125 1.9112633536476622 1106 1 -0.4789603413784711 1106 2 -0.17932253554728206 1106 3 -0.21542716796750128 1106 4 -0.5128070223293015 1106 5 -0.9489741536727294 1106 6 -0.47678352549973724 1106 7 -0.44457412753380865 1106 8 -0.09001649105849197 1106 9 0.07232503871851392 1106 10-11 -2.4179491525191494 1106 12-13 -0.5788596900990939 1106 14-15 -0.2965076441382024 1106 16-17 -0.5177768416911555 1106 18-19 -3.2532225608555088 1106 20-21 -5.729842322129002 1106 22-23 -6.539300021255901 1106 24-25 -6.366859967146507 1106 26-27 -5.365382024742111 1106 28-29 -1.8909800754295922 1106 30-31 -3.8939564372776303 1106 32-33 -4.370585939323675 1106 34-35 -3.6306834230564853 1106 36-37 -6.035159944059853 1106 38-39 -6.507022448506483 1106 40-41 -2.7702223552339724 1106 42-43 -1.332820743133862 1106 44-45 -0.5460924082529104 1106 46-47 -0.38072543204224374 1106 48-49 -0.3066583452423153 1106 50-51 -0.3631376189738056 1106 52-53 -0.5376039507505617 1106 54-55 -0.5112265674403638 1106 56-57 -0.35375729940620104 1106 58-59 -0.21370312789622758 1106 60-61 -0.04882276873015812 1106 62-63 -0.25062923150371574 1106 64-65 -0.5028119734028671 1106 66-67 -0.8064284232283363 1106 68-69 -0.6843907294565241 1106 70-71 -0.4882418371355044 1106 72-73 -0.45279573366394743 1106 74-75 -0.5166214907730939 1106 76-77 -0.5689329320982779 1106 78-79 -0.5536023432803745 1106 80-81 -0.9048676601008374 1106 82-83 -0.9423867523170166 1106 84-85 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108-109 -8.650050359830836 2216 110-111 -6.0110993759905185 2216 112-113 -5.461194025880417 2216 114-115 -5.822380960368182 2216 116-117 -5.388897644896694 2216 118-119 -5.646932597290558 2216 120-121 -6.358300844945248 2216 122-123 -9.403814415174025 2216 124-125 -8.002269234106164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 278210.0 3 43548.0 4 25722.0 5 22696.0 6 23970.0 7 25967.0 8 28194.0 9 30506.0 10 32380.0 11 33640.0 12 34237.0 13 36382.0 14 38183.0 15 32105.0 16 25241.0 17 22650.0 18 20806.0 19 19770.0 20 19907.0 21 21375.0 22 26457.0 23 35728.0 24 47840.0 25 60296.0 26 72940.0 27 83410.0 28 95623.0 29 108901.0 30 123932.0 31 140795.0 32 167878.0 33 205106.0 34 257705.0 35 328716.0 36 434563.0 37 474952.0 38 148201.0 39 341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28182705110053 19.60463211748938 14.152794339246363 22.960746492163725 2 14.814020523527189 21.276144728162638 40.580868689836834 23.328966058473334 3 17.916619341207554 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22.330580867565526 27.7154038038432 28.133923568708152 21.82009175988312 24-25 22.114351370695672 27.829232780281625 28.217046956892723 21.839368892129976 26-27 21.92661860114676 28.172743201481097 28.049708735295525 21.850929462076614 28-29 21.744429916898117 27.87213793443644 28.40692433472404 21.9765078139414 30-31 21.86118173435751 27.833745131313307 28.33619745579946 21.968875678529717 32-33 21.86297861293707 27.962591999479702 28.050102943514858 22.12432644406837 34-35 22.173964153086732 27.787455881787515 28.120047015327387 21.918532949798365 36-37 22.331551745281132 27.469483587481758 28.021266071150535 22.17769859608657 38-39 22.001145344680953 27.940361053650964 28.057610065804955 22.00088353586312 40-41 22.01096308576228 27.84154067038535 28.30894174701214 21.83855449684023 42-43 22.018111835998564 27.750618996068848 27.847409476647915 22.38385969128467 44-45 21.950188008816966 27.892011487435276 27.847885280137273 22.309915223610485 46-47 22.07286633822641 27.927140551165095 27.611441074689957 22.388552035918533 48-49 22.217471380811922 27.932269689910456 27.445015249695683 22.405243679581943 50-51 22.254846261359067 28.12662212079802 27.186548215131438 22.431983402711474 52-53 22.41059881893914 28.11813640143668 27.13915531618629 22.33210946343789 54-55 22.349146944149524 27.935131515217073 27.185098272116655 22.53062326851675 56-57 22.16911612730765 28.1588534705362 27.101956904557845 22.570073497598305 58-59 22.239416737542367 27.711402049047273 27.312150915357602 22.73703029805276 60-61 22.402211083008094 27.877682001486054 27.48800246545734 22.23210445004851 62-63 22.150368016261723 28.014734980890932 27.53210290124906 22.302794101598284 64-65 22.210753353128503 27.911667753154845 27.234407378915133 22.643171514801523 66-67 22.076885406329293 28.26547605826015 26.976997597709985 22.680640937700574 68-69 22.096451247663897 28.06667453921805 27.218905477435563 22.617968735682485 70-71 22.167642645942536 27.704964948659295 27.621230845841204 22.50616155955697 72-73 22.282924027870536 27.894163730360344 27.19162721087573 22.63128503089339 74-75 22.150073253547387 28.344527698907186 26.906655566684012 22.598743480861415 76-77 22.136914309021062 27.987416703870892 27.364452601815564 22.511216385292478 78-79 22.132336231395122 27.97278035188801 27.358797524168544 22.536085892548318 80-81 22.161020242031356 28.401895289499024 26.955185563436366 22.481898905033255 82-83 22.33096248451765 28.10284649292445 27.053580889283946 22.512610133273956 84-85 22.18361437011262 27.99829860900125 26.972696587614266 22.845390433271866 86-87 22.103005178891244 27.97691172957 27.250026110783022 22.67005698075573 88-89 22.22630541312614 27.76183082747586 27.25726968736445 22.75459407203355 90-91 22.14100949323615 27.904081897263833 27.320532476617583 22.634376132882434 92-93 22.379687827889086 27.917359446285197 27.061442771913963 22.641509953911758 94-95 22.2681299893752 27.623382941922387 27.1502754305277 22.958211638174717 96-97 22.312742674636176 27.6924656156392 27.181143784635818 22.813647925088805 98-99 22.084269768970977 27.729793417165766 27.154008647243373 23.03192816661989 100-101 22.0371409639532 27.75796328066344 27.22304764127721 22.98184811410615 102-103 22.36007403434112 27.434882035153496 27.27812466544213 22.92691926506325 104-105 22.169133487776907 27.696018891481323 27.202994550014875 22.9318530707269 106-107 22.07188655886829 27.633797829473174 27.386308444083756 22.908007167574784 108-109 22.160792720280224 27.791698736311325 26.870462885879466 23.17704565752899 110-111 21.917332569807876 27.900981073258485 27.192299562855954 22.98938679407768 112-113 21.96873734483624 27.846796096625614 27.28477764735175 22.8996889111864 114-115 21.838964686572645 28.17249234624738 26.989297078311953 22.99924588886802 116-117 21.677921697832556 28.195220650190766 27.178995299521986 22.947862352454692 118-119 21.960294433143446 27.98210285649092 26.92094808232785 23.136654628037785 120-121 21.80146136311306 28.148622670708818 27.03069794026537 23.01921802591276 122-123 21.85626353875427 28.08946776720666 27.133368708686174 22.920899985352897 124-125 22.026453581468296 28.24735889486271 26.65585101755838 23.070336506110618 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2558.0 1 2586.0 2 3230.0 3 3509.0 4 2685.0 5 1944.0 6 1656.5 7 1808.0 8 2289.5 9 2542.0 10 2300.0 11 2035.5 12 2063.5 13 2255.0 14 2462.5 15 2661.0 16 2953.5 17 3323.5 18 3682.0 19 4112.0 20 4632.5 21 5496.5 22 6854.5 23 8623.5 24 10836.0 25 14005.5 26 18679.5 27 24133.5 28 29707.5 29 36047.0 30 44413.5 31 55260.0 32 66246.0 33 76324.0 34 86656.5 35 96608.5 36 108613.5 37 118891.0 38 122272.0 39 123276.5 40 122263.0 41 121279.5 42 121313.0 43 122993.5 44 125868.5 45 125952.0 46 122887.5 47 120948.0 48 123258.5 49 122010.5 50 117221.0 51 113567.5 52 111078.5 53 110095.5 54 106546.0 55 99389.5 56 90907.0 57 82616.0 58 77210.5 59 72318.5 60 63582.0 61 52264.0 62 42515.5 63 33458.0 64 25733.0 65 20509.0 66 15913.5 67 12283.0 68 9467.0 69 7342.5 70 5795.5 71 4549.5 72 3717.0 73 3251.5 74 2635.0 75 2090.0 76 1525.5 77 1021.5 78 737.5 79 513.0 80 345.0 81 239.0 82 169.0 83 123.5 84 102.5 85 76.5 86 49.0 87 39.0 88 30.5 89 19.5 90 17.5 91 15.0 92 12.0 93 13.0 94 14.5 95 8.5 96 1.5 97 2.0 98 2.5 99 3.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09617310939236508 2 0.031249371361301428 3 0.04158315818712862 4 0.048444792639477874 5 0.05197205854269355 6 0.0023423250138541636 7 0.0016258491272634781 8 0.001928973540821076 9 0.004767320322314945 10-11 0.06496507317836694 12-13 0.005125558265610287 14-15 0.009079954024293492 16-17 0.017677664663381717 18-19 0.015707355975257332 20-21 0.12594819383318182 22-23 0.013502814785747531 24-25 0.012676111839681356 26-27 0.00592470444680759 28-29 0.003706384874863353 30-31 0.0019565303056899483 32-33 0.005070444735872542 34-35 0.01913817320143196 36-37 0.0024249953084607813 38-39 0.0074678832794644505 40-41 0.031786728276244444 42-43 0.011491170950319839 44-45 0.11141200036485156 46-47 0.00290723869366605 48-49 0.007605667103808814 50-51 0.009052397259424621 52-53 0.009796429910884178 54-55 0.008280807843096191 56-57 0.005042887971003669 58-59 0.012538328015336992 60-61 0.03164894445190008 62-63 0.006310499154971805 64-65 0.005773142240028791 66-67 0.006944304746955872 68-69 0.0063793910671439856 70-71 0.003430817226174628 72-73 6.200272095496315E-4 74-75 0.0010609354474515917 76-77 0.006971861511824746 78-79 0.0209018061530398 80-81 0.0023974385435919085 82-83 0.003981952523552078 84-85 0.0021356492773376198 86-87 0.0028659035463627414 88-89 0.00482243385205269 90-91 0.003623714580256735 92-93 0.0020254222178621296 94-95 0.002741898104452815 96-97 0.001184940889361518 98-99 0.0023836601611574725 100-101 0.0021080925124687473 102-103 4.546866203363965E-4 104-105 0.004064622818158696 106-107 8.404813285006116E-4 108-109 0.0014329517731813707 110-111 0.002535222367936271 112-113 0.004588201350667273 114-115 0.00268678457471507 116-117 0.005497574591340066 118-119 0.00378905516946997 120-121 0.007564331956505505 122-123 0.004670871645273891 124-125 0.05592645430137676 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3628873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.85314846836491 #Duplication Level Percentage of deduplicated Percentage of total 1 74.64804631275179 33.481999041392356 2 10.743469395427743 9.637568557169104 3 4.774087776933273 6.4239860357938285 4 2.7091880279396228 4.860624513835705 5 1.6121625165903213 3.615528235587924 6 1.1027401993591994 2.967682193033549 7 0.8035900068767841 2.523047932029714 8 0.5829668006909271 2.091831717081428 9 0.44065435984387513 1.7788261882778666 >10 2.3369755747258454 18.537441003790946 >50 0.1488357091695996 4.623175841823824 >100 0.0926050738624348 7.549951452507534 >500 0.00382166560719529 1.1578702247289696 >1k 7.906894351335164E-4 0.5945396725595951 >5k 6.589078626112636E-5 0.15592739038745454 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5276 0.14538949144817137 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3708 0.10218048413377927 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.267029460661754E-5 0.0 0.0 0.0 0.0 3 8.267029460661754E-5 0.0 0.0 0.0 0.0 4 8.267029460661754E-5 0.0 0.0 0.0 0.0 5 1.6534058921323507E-4 0.0 0.0 0.0 0.0 6 2.204541189509801E-4 0.0 0.0 2.755676486887251E-5 0.0 7 3.3068117842647015E-4 0.0 0.0 2.755676486887251E-5 0.0 8 3.582379432953427E-4 0.0 0.0 2.755676486887251E-5 0.0 9 3.582379432953427E-4 0.0 0.0 2.755676486887251E-5 0.0 10-11 3.8579470816421516E-4 0.0 0.0 4.133514730330877E-5 0.0 12-13 5.511352973774503E-4 0.0 0.0 5.511352973774502E-5 1.3778382434436256E-5 14-15 7.440326514595578E-4 0.0 0.0 6.889191217218128E-5 4.133514730330877E-5 16-17 7.853677987628665E-4 0.0 0.0 1.1022705947549006E-4 5.511352973774502E-5 18-19 9.64486770410538E-4 0.0 0.0 1.9289735408210758E-4 1.240054419099263E-4 20-21 0.0011022705947549005 0.0 0.0 3.582379432953426E-4 1.3778382434436257E-4 22-23 0.001129827359623773 0.0 0.0 8.129245636317392E-4 1.3778382434436257E-4 24-25 0.0015707355975257333 0.0 0.0 0.0014742869204846794 1.3778382434436257E-4 26-27 0.0015707355975257333 0.0 0.0 0.0026730061922806336 1.3778382434436257E-4 28-29 0.0021769844246409286 0.0 0.0 0.004326412084412984 1.3778382434436257E-4 30-31 0.0023423250138541636 0.0 0.0 0.008776829610735896 1.3778382434436257E-4 32-33 0.0023974385435919085 0.0 0.0 0.016754513040274487 1.9289735408210758E-4 34-35 0.00268678457471507 0.0 0.0 0.027680770310782442 1.9289735408210758E-4 36-37 0.0028659035463627414 0.0 0.0 0.04599224056614823 1.9289735408210758E-4 38-39 0.0031414711950514666 0.0 0.0 0.07576732500696498 1.9289735408210758E-4 40-41 0.0031414711950514666 0.0 0.0 0.11345120096514813 1.9289735408210758E-4 42-43 0.0034170388437401913 0.0 0.0 0.1491647682352069 1.9289735408210758E-4 44-45 0.0036374929626911716 0.0 0.0 0.19215332143064803 1.9289735408210758E-4 46-47 0.004381525614150729 0.0 0.0 0.2454894398343508 2.0667573651654385E-4 48-49 0.004519309438495092 0.0 0.0 0.30400623003340155 3.031244135575976E-4 50-51 0.004519309438495092 0.0 0.0 0.36698721614120966 3.031244135575976E-4 52-53 0.004546866203363965 0.0 0.0 0.43196606770201107 3.031244135575976E-4 54-55 0.0046019797331017095 0.0 0.0 0.5015744557607831 3.169027959920339E-4 56-57 0.005015331206134797 0.0 0.0 0.5858292643473608 3.3068117842647015E-4 58-59 0.00504288797100367 0.0 0.0 0.6842758068414078 3.3068117842647015E-4 60-61 0.0050842231183069785 0.0 0.0 0.7907551462947312 3.444595608609064E-4 62-63 0.005166893412913596 0.0 0.0 0.9010510976823933 3.582379432953427E-4 64-65 0.0052357853250857775 0.0 0.0 1.0155494557125586 3.582379432953427E-4 66-67 0.005511352973774503 0.0 0.0 1.14786326223045 3.7201632572977895E-4 68-69 0.005731807092725483 0.0 0.0 1.3050883841898022 3.8579470816421516E-4 70-71 0.005966039594110899 0.0 0.0 1.4818788092060537 4.82243385205269E-4 72-73 0.00614515856575857 0.0 0.0 1.6559824496475903 6.062488271151952E-4 74-75 0.006269164007668497 0.0 0.0 1.8418528286881353 6.062488271151952E-4 76-77 0.00629672077253737 0.0 0.0 2.048528565204679 6.200272095496315E-4 78-79 0.006310499154971805 0.0 0.0 2.2922957072347256 6.338055919840678E-4 80-81 0.0073163210726856515 0.0 0.0 2.5597341102871334 6.338055919840678E-4 82-83 0.007495440044333323 0.0 0.0 2.84589733506794 6.338055919840678E-4 84-85 0.007633223868677686 0.0 0.0 3.1307378351350406 6.338055919840678E-4 86-87 0.0077985644578909205 0.0 0.0 3.4327737564803176 6.338055919840678E-4 88-89 0.007826121222759793 0.0 0.0 3.7693796393535957 6.613623568529403E-4 90-91 0.008446148432309425 0.0 0.0 4.145708598785353 6.613623568529403E-4 92-93 0.008597710639088223 2.755676486887251E-5 0.0 4.55626306018425 6.889191217218128E-4 94-95 0.008597710639088223 5.511352973774502E-5 0.0 4.968526041005017 7.440326514595578E-4 96-97 0.008597710639088223 5.511352973774502E-5 0.0 5.3913157060056935 7.440326514595578E-4 98-99 0.00909373240672793 5.511352973774502E-5 0.0 5.842378611761834 7.440326514595578E-4 100-101 0.009575975791933197 5.511352973774502E-5 0.0 6.3527023403684835 7.440326514595578E-4 102-103 0.00997554888253185 5.511352973774502E-5 0.0 6.896149300347519 7.440326514595578E-4 104-105 0.01151872771518871 5.511352973774502E-5 0.0 7.4443360238839995 7.440326514595578E-4 106-107 0.011987192717959543 5.511352973774502E-5 0.0 8.000114636141856 7.440326514595578E-4 108-109 0.012152533307172777 8.267029460661754E-5 0.0 8.576023465136421 7.578110338939941E-4 110-111 0.012345430661254885 8.267029460661754E-5 0.0 9.213025641845277 7.991461811973029E-4 112-113 0.01244187933829594 8.267029460661754E-5 0.0 9.886554310387826 8.267029460661754E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15220 0.0 39.969788 1 GTACATG 16490 0.0 34.942207 1 GGTATCA 9705 0.0 34.77628 1 ATCAACG 17635 0.0 32.25643 3 TACATGG 17375 0.0 32.156494 2 CAACGCA 17880 0.0 31.68868 5 TCAACGC 17830 0.0 31.610905 4 AACGCAG 17560 0.0 31.535797 6 TATCAAC 19245 0.0 31.134329 2 ACATGGG 17160 0.0 29.612461 3 GAGTACT 7330 0.0 27.46894 12-13 ACGCAGA 19800 0.0 27.36778 7 CGCAGAG 19675 0.0 26.695692 8 CATGGGA 10450 0.0 25.656261 4 CGCATAG 520 0.0 25.194553 9 GCAGAGT 19840 0.0 24.702763 9 AACGCAT 565 0.0 24.239563 6 AGAGTAC 15605 0.0 23.990074 10-11 GTACTTT 8615 0.0 23.759352 14-15 CATGGGG 7510 0.0 21.816742 4 >>END_MODULE