FastQCFastQC Report
Thu 26 May 2016
SRR1364476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3628873
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122490.3375428128788194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109470.3016639050195474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66660.18369339461590417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120350.044.5748941
GTATCAA189650.041.785151
GTACATG159300.032.4773061
TATCAAC245600.032.0632172
ATCAACG236650.031.864763
CAACGCA236700.031.5217935
TCAACGC240450.031.237224
AACGCAG237350.030.8839726
TACATGG171200.029.2488062
ACATGGG166200.027.9404283
GAGTACT151750.027.90393312-13
ACGCAGA270550.026.8521947
CGCAGAG275950.026.5072618
GTACTTT165000.025.91564814-15
GCAGAGT280900.025.1933219
CATGGGG73400.024.5266084
AGAGTAC234000.024.39885510-11
AGTACTT168600.024.12751812-13
CAGAGTA277250.022.05137410-11
CATGGGA98950.021.8684224
Produced by FastQC (version 0.11.5)