##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364476_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3628873 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.88995757084913 31.0 28.0 31.0 25.0 34.0 2 29.104796447822782 31.0 28.0 33.0 25.0 34.0 3 29.184851329875695 31.0 28.0 34.0 25.0 34.0 4 32.59570616001166 35.0 32.0 37.0 26.0 37.0 5 31.70256716065842 35.0 32.0 37.0 20.0 37.0 6 31.723277998430916 35.0 32.0 37.0 19.0 37.0 7 31.15734912740126 35.0 31.0 37.0 17.0 37.0 8 31.270694510389315 35.0 32.0 37.0 17.0 37.0 9 31.828880206058464 37.0 30.0 39.0 11.0 39.0 10-11 32.03310270158256 37.0 31.0 39.0 14.0 39.0 12-13 31.782090472717012 37.0 30.5 39.0 11.0 39.0 14-15 32.70637026977798 38.0 31.0 40.0 10.5 41.0 16-17 32.777971287504414 38.0 31.0 40.0 11.0 41.0 18-19 32.65298413584603 38.0 31.0 40.0 10.0 41.0 20-21 32.54274784485431 38.0 30.5 40.0 10.0 41.0 22-23 32.50508160522564 38.0 30.5 40.0 10.0 41.0 24-25 32.29810315764702 37.5 30.0 40.0 9.5 41.0 26-27 32.377675796314726 37.0 30.0 40.0 9.5 41.0 28-29 32.255637631848785 37.0 30.0 40.0 8.5 41.0 30-31 31.7156898298728 37.0 30.0 40.0 8.0 41.0 32-33 31.61836278095155 37.0 30.0 40.0 4.0 41.0 34-35 31.390416666551847 36.5 30.0 40.0 2.0 41.0 36-37 31.354874226791623 36.0 30.0 40.0 2.0 41.0 38-39 31.10641926570591 36.0 30.0 40.0 2.0 41.0 40-41 30.80693096727276 36.0 28.5 40.0 2.0 41.0 42-43 30.670037364217485 36.0 28.5 40.0 2.0 41.0 44-45 30.566204163110697 36.0 28.0 40.0 2.0 41.0 46-47 30.359210559311393 36.0 28.0 40.0 2.0 41.0 48-49 30.041233187273292 36.0 27.0 40.0 2.0 41.0 50-51 29.685948364685125 35.0 26.5 40.0 2.0 41.0 52-53 29.59000907444267 35.0 26.5 40.0 2.0 41.0 54-55 29.115146768707532 35.0 25.0 39.5 2.0 41.0 56-57 29.124741758667223 35.0 26.0 39.0 2.0 41.0 58-59 28.877405326667535 35.0 25.5 39.0 2.0 41.0 60-61 28.59599151031188 34.5 24.5 39.0 2.0 41.0 62-63 28.329429550166125 34.0 24.0 39.0 2.0 40.5 64-65 27.85361998063862 34.0 23.0 38.0 2.0 40.0 66-67 27.453141099178723 34.0 22.0 38.0 2.0 40.0 68-69 27.261132726331287 33.5 21.5 37.0 2.0 40.0 70-71 27.342003977543442 34.0 22.5 37.0 2.0 40.0 72-73 27.11469428662838 34.0 22.0 36.5 2.0 39.0 74-75 26.7564651614978 33.0 21.5 36.0 2.0 39.0 76-77 26.68237452784928 33.0 22.0 36.0 2.0 39.0 78-79 26.28574697984746 33.0 20.0 35.0 2.0 37.5 80-81 26.05786700168344 33.0 20.0 35.0 2.0 37.0 82-83 25.922762934938753 33.0 20.0 35.0 2.0 37.0 84-85 25.48215561690916 33.0 18.0 35.0 2.0 36.0 86-87 25.362271840320673 33.0 18.0 35.0 2.0 36.0 88-89 25.321375672281725 33.0 18.0 35.0 2.0 36.0 90-91 25.158655180272223 32.5 18.0 35.0 2.0 35.0 92-93 25.173520677080738 32.5 18.0 35.0 2.0 35.0 94-95 25.191095555011156 33.0 18.0 35.0 2.0 35.0 96-97 25.068168960445846 33.0 18.0 35.0 2.0 35.0 98-99 24.933625536082417 32.5 16.0 35.0 2.0 35.0 100-101 24.839776564239088 32.0 13.5 35.0 2.0 35.0 102-103 23.723718768884993 30.5 10.5 34.0 2.0 35.0 104-105 24.662767751861253 32.0 15.0 35.0 2.0 35.0 106-107 24.750304020008414 32.5 10.0 35.0 2.0 35.0 108-109 24.823922744058557 33.0 10.0 35.0 2.0 35.0 110-111 24.75435265990295 33.0 9.0 35.0 2.0 35.0 112-113 24.707767122189175 33.0 7.0 35.0 2.0 35.0 114-115 24.654409785076524 33.0 4.5 35.0 2.0 35.0 116-117 24.570767976724454 33.0 2.0 35.0 2.0 35.0 118-119 24.447067863769277 33.0 2.0 35.0 2.0 35.0 120-121 24.28363792284822 33.0 2.0 35.0 2.0 35.0 122-123 23.998874581722752 32.5 2.0 35.0 2.0 35.0 124-125 23.2271480704891 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9357305205067696 1101 2 0.8782572751314142 1101 3 0.9049405614091626 1101 4 0.9209956524977372 1101 5 1.1973433662389752 1101 6 1.217597030078462 1101 7 1.6599925560435622 1101 8 1.5512563954247263 1101 9 2.115015954865566 1101 10-11 1.7049667723559736 1101 12-13 1.908111243281855 1101 14-15 1.8340365166642982 1101 16-17 1.7893629076986741 1101 18-19 1.8880548419015852 1101 20-21 2.147879195436481 1101 22-23 2.0848962942995186 1101 24-25 2.22357936543046 1101 26-27 1.868350116680432 1101 28-29 1.9629662091131053 1101 30-31 2.3961140962994456 1101 32-33 2.2333296241875615 1101 34-35 2.2398657658040513 1101 36-37 2.1054896147288886 1101 38-39 2.1563730677248536 1101 40-41 2.3809857111105757 1101 42-43 2.3565266881466513 1101 44-45 2.212008777704405 1101 46-47 2.0131811406759077 1101 48-49 2.0463055355486084 1101 50-51 2.3007769842997874 1101 52-53 2.217263617245596 1101 54-55 2.5577559251303263 1101 56-57 2.3150908141696185 1101 58-59 2.3339166920311563 1101 60-61 2.4067749365932904 1101 62-63 2.429699985677832 1101 64-65 2.6269622824470815 1101 66-67 2.742874087247717 1101 68-69 2.737648671497599 1101 70-71 2.3227006231383953 1101 72-73 2.5494055862504226 1101 74-75 2.598486337562587 1101 76-77 2.2952768716315752 1101 78-79 2.391875784119904 1101 80-81 2.4809934897014863 1101 82-83 2.393915841882304 1101 84-85 2.625945214100849 1101 86-87 2.540509541345223 1101 88-89 2.3910604331342036 1101 90-91 2.349184750796489 1101 92-93 2.268981881728397 1101 94-95 2.2174248943815797 1101 96-97 2.107315021158403 1101 98-99 2.206570131864492 1101 100-101 2.371240539734348 1101 102-103 2.1458059796876405 1101 104-105 2.2759140413553958 1101 106-107 2.3141925986189555 1101 108-109 2.3027743092930173 1101 110-111 2.253680639130085 1101 112-113 2.120848616829406 1101 114-115 2.1074568804347678 1101 116-117 2.1922370031188443 1101 118-119 2.1568098215840052 1101 120-121 2.1978305773669824 1101 122-123 2.2793561572291132 1101 124-125 2.1431681704087246 1102 1 0.4547940155942669 1102 2 -1.686344275147679 1102 3 0.005431941043507749 1102 4 0.4732133732686563 1102 5 0.8511492741994147 1102 6 0.42814513577530633 1102 7 1.3089779150947862 1102 8 0.9633855602931867 1102 9 1.7666642118059919 1102 10-11 1.3729505671312694 1102 12-13 1.6367006538984548 1102 14-15 1.5578170100009032 1102 16-17 1.4725781751354248 1102 18-19 1.563992889057964 1102 20-21 1.6607145200788125 1102 22-23 1.6710472683107653 1102 24-25 1.9034739809257317 1102 26-27 1.5945306784460058 1102 28-29 1.4983098054962198 1102 30-31 1.8811177510286292 1102 32-33 1.4520777368828206 1102 34-35 1.7190009233915724 1102 36-37 1.231577285461121 1102 38-39 1.1796208389232525 1102 40-41 1.3985727668892594 1102 42-43 1.2287255391101652 1102 44-45 1.1853593219609735 1102 46-47 0.8177964026694475 1102 48-49 0.8932335542317915 1102 50-51 1.060603604984255 1102 52-53 0.8488157561045675 1102 54-55 1.3249277414659275 1102 56-57 1.005798244026682 1102 58-59 0.845295438614567 1102 60-61 0.8605405712525638 1102 62-63 1.2162413482697367 1102 64-65 1.3410966883527102 1102 66-67 1.3946930602169765 1102 68-69 1.3570188009553625 1102 70-71 0.7486342314836243 1102 72-73 1.066653218776473 1102 74-75 1.3072721380325163 1102 76-77 1.1265790438626304 1102 78-79 0.9587781289785937 1102 80-81 0.9590437305636961 1102 82-83 0.5771002968416568 1102 84-85 0.6701267102847659 1102 86-87 0.813515672736628 1102 88-89 0.9824330042621021 1102 90-91 0.7473890410856647 1102 92-93 0.7490337996188146 1102 94-95 0.8709982669578906 1102 96-97 0.9642222325093179 1102 98-99 0.7431518649578059 1102 100-101 0.5266789257955757 1102 102-103 0.3951130067468931 1102 104-105 0.6444372650857346 1102 106-107 0.9215476438948293 1102 108-109 0.840213672540699 1102 110-111 0.7384786829427696 1102 112-113 0.7297976489093756 1102 114-115 1.0027175909296737 1102 116-117 0.5908825825165387 1102 118-119 0.25296506592597723 1102 120-121 0.5238747995900681 1102 122-123 0.9005908465706547 1102 124-125 0.8744046352747255 1103 1 0.6477513302942057 1103 2 0.5633337055212309 1103 3 0.5556162472580226 1103 4 0.5112076821515785 1103 5 0.8986636949954772 1103 6 0.7658300306100863 1103 7 1.1583535291418414 1103 8 0.9620810176182992 1103 9 1.727300972398833 1103 10-11 1.3776949425643323 1103 12-13 1.2932568745815622 1103 14-15 1.158562435500471 1103 16-17 1.1325045231238846 1103 18-19 1.3770164047178284 1103 20-21 1.6373927925752412 1103 22-23 1.433624874088629 1103 24-25 1.7682541636911253 1103 26-27 1.1690354606052509 1103 28-29 1.405608392455207 1103 30-31 1.8849135588991324 1103 32-33 1.666178852073962 1103 34-35 1.7128304211631438 1103 36-37 1.5882160571331596 1103 38-39 1.7462539502306278 1103 40-41 2.0118168509753005 1103 42-43 1.955460303135105 1103 44-45 1.7496777221183208 1103 46-47 1.7061205791536587 1103 48-49 1.7126739117028826 1103 50-51 1.8956553090679513 1103 52-53 1.7320228924565697 1103 54-55 1.9806228307231848 1103 56-57 1.8386322672069504 1103 58-59 2.0543904145363925 1103 60-61 1.903628151220829 1103 62-63 1.8874182236138992 1103 64-65 1.9442117468767819 1103 66-67 2.2120039417314814 1103 68-69 2.2857442066388565 1103 70-71 2.085566765510677 1103 72-73 2.1163096887783013 1103 74-75 2.0731266048354584 1103 76-77 1.9875625538461144 1103 78-79 2.133647935786218 1103 80-81 2.029857631419862 1103 82-83 1.9356667157912852 1103 84-85 2.118734539270637 1103 86-87 2.0475258152644145 1103 88-89 1.9220329891421706 1103 90-91 1.9803977763705625 1103 92-93 1.8210944542542435 1103 94-95 1.6675382849672644 1103 96-97 1.5572670153797148 1103 98-99 1.7446510287166106 1103 100-101 1.7736765139740918 1103 102-103 1.6381182919962782 1103 104-105 1.5305860131905398 1103 106-107 1.5446216063134663 1103 108-109 1.5436702109025653 1103 110-111 1.5972491409005478 1103 112-113 1.5203788597661259 1103 114-115 1.452616348244181 1103 116-117 1.446146305216974 1103 118-119 1.5276956219967417 1103 120-121 1.5760173293021786 1103 122-123 1.614639538520283 1103 124-125 1.4719772133546023 1104 1 0.7113820994172464 1104 2 0.6768073024050629 1104 3 0.635083854612926 1104 4 0.659545787229689 1104 5 0.9224153907765924 1104 6 0.9784548367348442 1104 7 1.2085105595220327 1104 8 1.1094022048623238 1104 9 1.5204167175508694 1104 10-11 1.1234123623710488 1104 12-13 1.2373641070761359 1104 14-15 1.249276338561934 1104 16-17 1.17017544021855 1104 18-19 1.1100830642372088 1104 20-21 1.430544153394024 1104 22-23 1.438887692506654 1104 24-25 1.6081413531794837 1104 26-27 1.4750922337286525 1104 28-29 1.4369761212866976 1104 30-31 1.9652528747891758 1104 32-33 1.9319282885541256 1104 34-35 1.8228789680333755 1104 36-37 1.7339306005220507 1104 38-39 1.8111610128082951 1104 40-41 1.902680367001615 1104 42-43 1.9351389997176014 1104 44-45 1.6261377494029432 1104 46-47 1.7293532671401657 1104 48-49 1.3930138252117423 1104 50-51 1.7261416678601194 1104 52-53 1.5905460550299857 1104 54-55 1.801414771030604 1104 56-57 1.7070350346408425 1104 58-59 1.8471396956054882 1104 60-61 1.9668313210634665 1104 62-63 2.0091160212406685 1104 64-65 2.04555971836988 1104 66-67 2.0339836192509324 1104 68-69 2.1455841679421326 1104 70-71 1.998793947254736 1104 72-73 2.1455745237674435 1104 74-75 2.221186439879986 1104 76-77 2.0691942506063583 1104 78-79 2.1031863993028104 1104 80-81 1.9552955388702564 1104 82-83 1.9571722552196889 1104 84-85 2.24751149395426 1104 86-87 2.049902587547532 1104 88-89 1.9323565976176944 1104 90-91 2.0265460370870194 1104 92-93 1.6421222578055925 1104 94-95 1.6588374832193686 1104 96-97 1.7563136654742308 1104 98-99 1.852571116939636 1104 100-101 1.832224399253132 1104 102-103 1.7218987604068872 1104 104-105 1.624745777284435 1104 106-107 1.7865098288372572 1104 108-109 1.7276570026816351 1104 110-111 1.7982817417011105 1104 112-113 1.748590711531186 1104 114-115 1.6735587255060267 1104 116-117 1.6725123448698263 1104 118-119 1.640166713318422 1104 120-121 1.5844640223226243 1104 122-123 1.7053495329132282 1104 124-125 1.4442160537060786 1105 1 0.5830552885935845 1105 2 0.710523358853095 1105 3 0.558177102762027 1105 4 0.7340309291068721 1105 5 0.9506471780510992 1105 6 0.9947766840640391 1105 7 1.3734163367911094 1105 8 1.2782187254449333 1105 9 1.8910773482702048 1105 10-11 1.6037797023685982 1105 12-13 1.6953628756739114 1105 14-15 1.7220886355114047 1105 16-17 1.6455791339638068 1105 18-19 1.8649652170648992 1105 20-21 1.9674096294097296 1105 22-23 1.9147070801278119 1105 24-25 2.1194687267008945 1105 26-27 1.8749415505803029 1105 28-29 1.8659080113015065 1105 30-31 2.1228190527927957 1105 32-33 2.0441909224483297 1105 34-35 2.2013473336489646 1105 36-37 1.9690849696199564 1105 38-39 2.043309080517073 1105 40-41 2.176848183895835 1105 42-43 2.2050439925612437 1105 44-45 2.136982886456998 1105 46-47 2.0567045368590264 1105 48-49 2.202185003235396 1105 50-51 2.329724273019572 1105 52-53 2.287517787299997 1105 54-55 2.4914709460920186 1105 56-57 2.279775300449348 1105 58-59 2.207097533345266 1105 60-61 2.0977209779040464 1105 62-63 1.989895412271533 1105 64-65 2.3025510467045045 1105 66-67 2.534091401856287 1105 68-69 2.5483528740299413 1105 70-71 2.4250757603509285 1105 72-73 2.6124101454737847 1105 74-75 2.6207951621565897 1105 76-77 2.411083816740163 1105 78-79 2.5707686064209945 1105 80-81 2.513264411873113 1105 82-83 2.4705262331491618 1105 84-85 2.730092726004912 1105 86-87 2.6340372096457187 1105 88-89 2.5193183843202753 1105 90-91 2.5581878134244995 1105 92-93 2.380511046131783 1105 94-95 2.2223744563110266 1105 96-97 2.2673415821556198 1105 98-99 2.332972851442708 1105 100-101 2.3863542322275855 1105 102-103 2.1612140419980115 1105 104-105 1.8081501217628038 1105 106-107 2.039503638311974 1105 108-109 2.129664111810392 1105 110-111 2.2309942800131886 1105 112-113 2.1862528256160303 1105 114-115 2.1201898216361528 1105 116-117 2.1376067788856226 1105 118-119 2.242079560859267 1105 120-121 2.2707978497468453 1105 122-123 2.3183517245687675 1105 124-125 2.1276566292897776 1106 1 0.39225320033741795 1106 2 0.3415932262273884 1106 3 0.30769442957382154 1106 4 0.2989080576759662 1106 5 0.3129391404975017 1106 6 0.4989725967589642 1106 7 0.6332414286170405 1106 8 0.4157291940190504 1106 9 1.124281894987707 1106 10-11 0.7071239538366569 1106 12-13 0.6527937068602547 1106 14-15 0.8495673455175208 1106 16-17 0.8191693004135203 1106 18-19 0.8783930228763595 1106 20-21 0.6502920538601913 1106 22-23 0.5885943991745393 1106 24-25 0.4448066839925602 1106 26-27 0.5293294137715989 1106 28-29 0.6138013874910371 1106 30-31 1.0477016547725704 1106 32-33 0.7184790962913254 1106 34-35 0.806772329481209 1106 36-37 0.6872847240295457 1106 38-39 0.9328455839370982 1106 40-41 1.1983236369029413 1106 42-43 1.0880193494271246 1106 44-45 0.9804623970662227 1106 46-47 0.9707874184597571 1106 48-49 1.0017696534903529 1106 50-51 1.275169232293564 1106 52-53 0.9639507586735157 1106 54-55 1.3640021944476572 1106 56-57 1.032331525851582 1106 58-59 1.100325761292904 1106 60-61 1.2978697877937577 1106 62-63 1.4523008754533784 1106 64-65 1.493229208681072 1106 66-67 1.6533009218534147 1106 68-69 1.4267291889639928 1106 70-71 1.3352478949368347 1106 72-73 1.2400957681074445 1106 74-75 1.4858509388178263 1106 76-77 1.1353793179703295 1106 78-79 1.0759521795992164 1106 80-81 1.2735075704785892 1106 82-83 1.144482646014481 1106 84-85 1.3104940184773248 1106 86-87 1.4360692809800462 1106 88-89 1.166979374147008 1106 90-91 1.311353650391613 1106 92-93 1.2903715823489925 1106 94-95 1.1686631101224734 1106 96-97 1.076184127916136 1106 98-99 1.049618179348201 1106 100-101 0.919483242927047 1106 102-103 0.7741911028502386 1106 104-105 0.9586600636502318 1106 106-107 0.9555918389073135 1106 108-109 0.9534683584492605 1106 110-111 0.8884673912070262 1106 112-113 0.682090291874399 1106 114-115 0.8036217386527547 1106 116-117 1.0476583681172151 1106 118-119 0.7805537529656519 1106 120-121 0.7952954811320581 1106 122-123 0.9997487548623489 1106 124-125 1.0319083333990697 1107 1 0.34014345323709705 1107 2 0.4086811984107648 1107 3 0.2933878320306107 1107 4 0.2661260068760285 1107 5 0.19044670411623343 1107 6 0.525058583457966 1107 7 0.6914512209049235 1107 8 0.6097298133422022 1107 9 1.3083075652953902 1107 10-11 0.729299391328567 1107 12-13 1.1164262790687864 1107 14-15 0.9534102818099157 1107 16-17 0.9330327989450069 1107 18-19 1.024455859065263 1107 20-21 1.1571371150336631 1107 22-23 1.00506645044117 1107 24-25 1.3090764142917735 1107 26-27 1.2528191429414335 1107 28-29 1.0664905526799373 1107 30-31 1.4641119237775833 1107 32-33 1.2840083793378128 1107 34-35 1.2261958273316473 1107 36-37 1.049506988647856 1107 38-39 1.1328333867252987 1107 40-41 1.3487331422883884 1107 42-43 1.3272491930370052 1107 44-45 1.094613232876867 1107 46-47 1.2692237469318037 1107 48-49 0.9703423414618513 1107 50-51 1.3339989601968227 1107 52-53 0.9385266919669242 1107 54-55 1.5718018015155373 1107 56-57 1.365210845051788 1107 58-59 1.2294897785442878 1107 60-61 1.5723216145336991 1107 62-63 1.541205258989816 1107 64-65 1.6743616470663163 1107 66-67 1.6270562764692045 1107 68-69 1.661499425096867 1107 70-71 1.5318410878208404 1107 72-73 1.1959576017179998 1107 74-75 1.3947453459353412 1107 76-77 1.0833901190101933 1107 78-79 1.2537903449229795 1107 80-81 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104-105 -4.311589212508284 2216 106-107 -4.4688362395363335 2216 108-109 -4.421919132859362 2216 110-111 -4.114482148923322 2216 112-113 -4.129733125748732 2216 114-115 -3.9786994133775835 2216 116-117 -3.947207216036997 2216 118-119 -4.097107269329907 2216 120-121 -4.112186858343126 2216 122-123 -4.175528783011984 2216 124-125 -4.1714253399076675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 190248.0 3 63278.0 4 30429.0 5 25009.0 6 26116.0 7 31094.0 8 33860.0 9 35491.0 10 38105.0 11 40849.0 12 44591.0 13 48195.0 14 50574.0 15 40670.0 16 28228.0 17 24196.0 18 21463.0 19 19959.0 20 19456.0 21 20824.0 22 25267.0 23 33979.0 24 46967.0 25 62194.0 26 75563.0 27 84512.0 28 96011.0 29 108173.0 30 120902.0 31 137152.0 32 160979.0 33 190157.0 34 231150.0 35 295745.0 36 409857.0 37 523730.0 38 193309.0 39 591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.0321046504571 20.52162470827747 14.809104501492406 23.637166139773022 2 15.452078863377954 21.917528875475426 38.59599907188432 24.034393189262296 3 18.065333231557016 26.702946066175365 30.360748364574896 24.87097233769272 4 12.38842721262044 18.223189470891484 37.557089128750114 31.831294187737964 5 12.595590972734511 39.23758147501993 34.2422840369448 13.924543515300755 6 29.96114214161059 38.651320095225316 17.45564111332821 13.931896649835885 7 26.553851896429997 33.60483936107775 21.837277779447163 18.004030963045096 8 25.405408235559634 35.77127113569419 19.991743993245287 18.831576635500884 9 25.75248568908308 16.21112119382519 20.69209917238768 37.34429394470404 10-11 24.201509240127578 27.49597254884886 27.51307164867288 20.789446562350676 12-13 25.19199624563945 25.099818206719654 28.05448948384983 21.65369606379106 14-15 22.471099351048657 26.23712248589741 26.416848622616314 24.874929540437623 16-17 21.38102701715829 29.447758674736562 27.02815392511807 22.14306038298708 18-19 21.188292149526582 27.46471091235619 29.97879747454608 21.36819946357115 20-21 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29.16162831456513 22.278270253348737 46-47 21.91690356331506 26.977255717209886 28.673817840715067 22.432022878759987 48-49 22.07886580764882 27.10571574149881 28.24124735145044 22.574171099401934 50-51 22.103275531352395 27.216782515061578 27.910191258743765 22.769750694842262 52-53 22.28272925656492 27.34383503850096 27.798246341630772 22.575189363303352 54-55 22.151929210650962 27.114940525737826 27.804105463248835 22.929024800362374 56-57 22.074851461902572 27.390119285165316 27.64918086003152 22.88584839290059 58-59 22.060113429155553 26.997514104241176 27.963116923628906 22.97925554297436 60-61 22.24506065657299 27.195592130118634 28.100170493704248 22.45917671960413 62-63 22.0692520145859 27.20513882162312 28.25978495927426 22.465824204516725 64-65 22.07115934314325 27.00330650819472 27.933921123311123 22.991613025350908 66-67 21.97403009892593 27.3476350262951 27.627480127206834 23.050854747572135 68-69 21.993713093982272 27.151213675439784 27.858263255302283 22.996809975275657 70-71 22.103522534040845 26.978748006725706 28.09444424005905 22.823285219174398 72-73 22.10117356012998 27.007270687111713 27.733829232681977 23.157726520076327 74-75 21.912008372150062 27.485606061127992 27.46524062697428 23.137144939747664 76-77 21.876904032753426 27.112144997355887 27.908245979192635 23.102704990698054 78-79 21.935431601222273 27.135898364715676 27.826935957254406 23.101734076807645 80-81 21.762662140738616 27.415594225087762 27.628250013985074 23.193493620188548 82-83 21.962736578662156 27.153599181232046 27.647706570673435 23.23595766943236 84-85 21.664580389638928 26.981521119851966 27.5823413470713 23.771557143437803 86-87 21.65463283221712 27.033572889378238 27.839513478799365 23.472280799605276 88-89 21.74400684518622 26.883697282386663 27.738449953876287 23.63384591855083 90-91 21.679557977555245 26.992802571492387 27.873945727666126 23.453693723286246 92-93 21.783046139346723 27.043215507097408 27.675919521383165 23.497818832172708 94-95 21.577821264198192 26.75729368959129 27.806300239836702 23.85858480637382 96-97 21.696596253026144 26.78059796709376 27.823767945006537 23.69903783487356 98-99 21.33255972308758 26.792588222293805 27.704551798864273 24.170300255754334 100-101 21.312980208350986 26.835947193426197 27.875538896368347 23.975533701854467 102-103 21.641535010112094 26.481699438082266 27.863828023576794 24.01293752822885 104-105 21.450017519244646 26.641569757388428 27.922920283785587 23.98549243958134 106-107 21.15067208929059 26.55109902654283 28.070580088212022 24.227648795954554 108-109 21.422218609223663 26.648594019216716 27.4672918378918 24.46189553366783 110-111 21.0181746834721 26.71161917915691 27.772749424648165 24.49745671272283 112-113 21.097702893105264 26.568401890506383 27.90551270933175 24.428382507056607 114-115 20.839846502991087 26.83290411286944 27.695336187025944 24.631913197113533 116-117 20.788423843986827 26.7636949543288 27.759527544777672 24.688353656906703 118-119 20.737919919489322 26.58582898012324 27.745746534942135 24.930504565445304 120-121 20.559912444873298 26.652299197020945 27.846316667537018 24.94147169056874 122-123 20.604500381388252 26.613170350480374 27.802996984166878 24.9793322839645 124-125 20.45874162568069 26.615408761309702 27.628546550667153 25.297303062342458 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1246.0 1 950.0 2 954.0 3 1243.0 4 976.5 5 776.0 6 1312.0 7 2880.5 8 4628.0 9 5329.0 10 4372.0 11 2981.0 12 2557.5 13 2731.5 14 3023.0 15 3272.0 16 3657.0 17 4086.5 18 4611.5 19 5219.0 20 5797.0 21 6628.5 22 7837.0 23 9461.5 24 11388.5 25 14159.5 26 18650.5 27 23786.0 28 28989.0 29 35101.5 30 43260.5 31 53564.0 32 63703.5 33 73364.5 34 83350.5 35 93249.5 36 105660.0 37 116524.5 38 120663.0 39 122264.5 40 121809.5 41 120988.0 42 120848.0 43 123104.5 44 126024.0 45 126364.5 46 123535.5 47 121525.5 48 125085.5 49 124552.5 50 119496.0 51 114612.5 52 111651.5 53 111163.0 54 107774.0 55 100586.0 56 91788.5 57 83025.5 58 77966.5 59 73155.0 60 64279.5 61 52923.0 62 42759.5 63 33745.0 64 25941.0 65 20548.0 66 15671.5 67 12071.0 68 9388.5 69 7193.0 70 5517.0 71 4347.0 72 3661.5 73 3209.0 74 2626.5 75 2067.0 76 1473.5 77 959.5 78 660.0 79 409.0 80 254.5 81 175.0 82 113.0 83 74.5 84 55.0 85 37.0 86 21.5 87 14.5 88 12.0 89 8.0 90 5.0 91 4.5 92 3.0 93 1.0 94 0.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10617621503976578 2 4.1335147303308764E-4 3 0.0 4 0.18424452991328163 5 0.0 6 1.6534058921323507E-4 7 0.04001242258960289 8 0.0 9 0.0 10-11 7.440326514595579E-4 12-13 7.164758865906854E-4 14-15 5.098001500741415E-4 16-17 0.0044228607614540385 18-19 0.0016534058921323506 20-21 0.003306811784264701 22-23 0.001818746481345586 24-25 0.004863768999355998 26-27 0.004229963407371931 28-29 0.0033619253140024464 30-31 8.404813285006116E-4 32-33 6.889191217218128E-5 34-35 0.0012538328015336993 36-37 0.001350281478574753 38-39 6.062488271151953E-4 40-41 0.001667184274566787 42-43 8.404813285006116E-4 44-45 0.001129827359623773 46-47 1.3778382434436257E-4 48-49 0.0 50-51 0.03943373052735656 52-53 5.511352973774502E-5 54-55 0.13763226213758376 56-57 1.6534058921323507E-4 58-59 0.0 60-61 0.0 62-63 7.02697504156249E-4 64-65 0.06165826139410224 66-67 0.051434701627750545 68-69 0.0016809626570012232 70-71 0.002962352223403795 72-73 0.0716338102766341 74-75 0.004836212234487125 76-77 0.0017085194218700956 78-79 0.001501843685353552 80-81 1.1022705947549004E-4 82-83 1.6534058921323507E-4 84-85 1.3778382434436256E-5 86-87 2.0667573651654382E-4 88-89 0.0012538328015336993 90-91 2.0667573651654382E-4 92-93 0.0022872114841164183 94-95 0.004243741789806367 96-97 0.0021494276597720558 98-99 0.0 100-101 0.0012262760366648269 102-103 0.0013778382434436257 104-105 1.7911897164767134E-4 106-107 6.889191217218128E-5 108-109 1.3778382434436256E-5 110-111 1.7911897164767134E-4 112-113 1.1022705947549004E-4 114-115 2.0667573651654382E-4 116-117 0.0 118-119 0.0010333786825827192 120-121 0.0020254222178621296 122-123 6.889191217218128E-4 124-125 0.008115467253882954 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3628873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.69758440742735 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0552030342598 32.046758667719054 2 10.364855879305992 8.851086175549744 3 4.650212079563933 5.956584683388579 4 2.5419565434902167 4.341416163027432 5 1.6486706561531277 3.5197127250573397 6 1.1269375673699686 2.8870507142808046 7 0.771422382225642 2.305651059520062 8 0.5861459658543493 2.0021613481711285 9 0.4657002006460618 1.7895846263076967 >10 2.4921107165095444 19.062658476507195 >50 0.1768695843266304 5.227849142399766 >100 0.11357624087693771 8.98608591392877 >500 0.004937863758091198 1.4474667500678657 >1k 0.0012011019939208855 0.7251294430740219 >5k 6.67278885511603E-5 0.18992231611847984 >10k+ 1.334557771023206E-4 0.660881794882127 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12249 0.3375428128788194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10947 0.3016639050195474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6666 0.18369339461590417 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 8.267029460661754E-5 0.0 0.0 0.0 0.0 6 1.1022705947549004E-4 0.0 0.0 0.0 2.755676486887251E-5 7 3.031244135575976E-4 0.0 0.0 0.0 2.755676486887251E-5 8 3.582379432953427E-4 0.0 0.0 0.0 2.755676486887251E-5 9 3.582379432953427E-4 0.0 0.0 0.0 2.755676486887251E-5 10-11 4.409082379019602E-4 0.0 0.0 1.3778382434436256E-5 2.755676486887251E-5 12-13 6.200272095496315E-4 0.0 0.0 4.133514730330877E-5 2.755676486887251E-5 14-15 9.369300055416654E-4 0.0 0.0 6.889191217218128E-5 2.755676486887251E-5 16-17 0.001019600300148283 0.0 0.0 1.515622067787988E-4 2.755676486887251E-5 18-19 0.0012124976542303907 0.0 0.0 2.204541189509801E-4 2.755676486887251E-5 20-21 0.0013227247137058806 0.0 0.0 4.960217676397052E-4 2.755676486887251E-5 22-23 0.001405395008312498 0.0 0.0 0.001088492212320464 2.755676486887251E-5 24-25 0.0016809626570012232 0.0 0.0 0.0017360761867389683 2.755676486887251E-5 26-27 0.0017360761867389683 0.0 0.0 0.0028796819287971774 2.755676486887251E-5 28-29 0.0021907628070753646 0.0 0.0 0.004588201350667273 4.133514730330877E-5 30-31 0.002314768248985291 0.0 0.0 0.008763051228301458 5.511352973774502E-5 32-33 0.002328546631419727 1.3778382434436256E-5 0.0 0.016547837303757945 8.267029460661754E-5 34-35 0.0025076656030673986 2.755676486887251E-5 0.0 0.02708829986610168 1.1022705947549004E-4 36-37 0.002976130605838231 2.755676486887251E-5 0.0 0.04509664570790986 1.1022705947549004E-4 38-39 0.003334368549133574 2.755676486887251E-5 0.0 0.07449971382299683 1.240054419099263E-4 40-41 0.003334368549133574 5.511352973774502E-5 0.0 0.11076441639043305 1.3778382434436257E-4 42-43 0.0035272659032156814 5.511352973774502E-5 0.0 0.14581662130363887 1.3778382434436257E-4 44-45 0.003954395758683206 5.511352973774502E-5 0.0 0.18768912552189068 1.3778382434436257E-4 46-47 0.004381525614150729 5.511352973774502E-5 0.0 0.2392202758266823 1.791189716476713E-4 48-49 0.004574422968232837 5.511352973774502E-5 0.0 0.2965521251363715 2.7556764868872513E-4 50-51 0.004574422968232837 5.511352973774502E-5 0.0 0.35889930565219563 2.8934603112316134E-4 52-53 0.0046019797331017095 5.511352973774502E-5 0.0 0.42161850249374944 3.444595608609064E-4 54-55 0.004712206792577199 5.511352973774502E-5 0.0 0.48997305775098765 3.9957309059865143E-4 56-57 0.004877547381790435 5.511352973774502E-5 0.0 0.5717615358818013 4.133514730330877E-4 58-59 0.004877547381790435 6.889191217218128E-5 0.0 0.665936779821173 4.133514730330877E-4 60-61 0.004905104146659307 8.267029460661754E-5 0.0 0.7677590260116571 4.133514730330877E-4 62-63 0.004960217676397052 8.267029460661754E-5 0.0 0.8722818351592905 4.133514730330877E-4 64-65 0.005070444735872542 8.267029460661754E-5 0.0 0.9794363153519012 4.409082379019602E-4 66-67 0.00537356914943014 8.267029460661754E-5 0.0 1.1041995682957215 4.409082379019602E-4 68-69 0.00548379620890563 8.267029460661754E-5 0.0 1.2532265527065842 4.409082379019602E-4 70-71 0.005662915180553301 8.267029460661754E-5 0.0 1.4212952616418377 4.409082379019602E-4 72-73 0.005855812534635409 8.267029460661754E-5 0.0 1.5844175312831283 5.235785325085777E-4 74-75 0.005966039594110899 8.267029460661754E-5 0.0 1.7580664851043286 5.235785325085777E-4 76-77 0.00603493150628308 8.267029460661754E-5 0.0 1.9503024768295831 5.37356914943014E-4 78-79 0.0060762666535863884 8.267029460661754E-5 0.0 2.177466668026134 5.511352973774503E-4 80-81 0.00684785606991482 8.267029460661754E-5 0.0 2.4269931739137744 5.92470444680759E-4 82-83 0.006985639894259182 8.267029460661754E-5 0.0 2.6942524579945344 6.062488271151952E-4 84-85 0.0072887643078167795 8.267029460661754E-5 0.0 2.9626966829646557 6.062488271151952E-4 86-87 0.007633223868677686 8.267029460661754E-5 0.0 3.248308772448085 6.062488271151952E-4 88-89 0.007743450928153176 8.267029460661754E-5 0.0 3.5671818771282435 6.062488271151952E-4 90-91 0.008198137548489573 8.267029460661754E-5 2.755676486887251E-5 3.9262740801345215 6.062488271151952E-4 92-93 0.008363478137702808 8.267029460661754E-5 2.755676486887251E-5 4.3233119483652365 6.062488271151952E-4 94-95 0.008377256520137244 8.267029460661754E-5 2.755676486887251E-5 4.722816147051715 6.613623568529403E-4 96-97 0.008377256520137244 8.267029460661754E-5 2.755676486887251E-5 5.1321994459436855 6.613623568529403E-4 98-99 0.00872171608099815 8.267029460661754E-5 2.755676486887251E-5 5.5764833875420825 6.613623568529403E-4 100-101 0.009148845936465675 8.267029460661754E-5 2.755676486887251E-5 6.0794770166936125 6.613623568529403E-4 102-103 0.009562197409498763 8.267029460661754E-5 2.755676486887251E-5 6.620347419157408 7.440326514595579E-4 104-105 0.010581797709647045 8.267029460661754E-5 2.755676486887251E-5 7.164083725167566 7.715894163284303E-4 106-107 0.010898700505639078 8.267029460661754E-5 2.755676486887251E-5 7.716789758142542 7.715894163284303E-4 108-109 0.011008927565114568 8.267029460661754E-5 2.755676486887251E-5 8.290425154035427 7.853677987628665E-4 110-111 0.011105376242155622 8.267029460661754E-5 2.755676486887251E-5 8.924258302784363 8.404813285006116E-4 112-113 0.01118804653676224 8.267029460661754E-5 2.755676486887251E-5 9.589919514956847 8.818164758039204E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12035 0.0 44.574894 1 GTATCAA 18965 0.0 41.78515 1 GTACATG 15930 0.0 32.477306 1 TATCAAC 24560 0.0 32.063217 2 ATCAACG 23665 0.0 31.86476 3 CAACGCA 23670 0.0 31.521793 5 TCAACGC 24045 0.0 31.23722 4 AACGCAG 23735 0.0 30.883972 6 TACATGG 17120 0.0 29.248806 2 ACATGGG 16620 0.0 27.940428 3 GAGTACT 15175 0.0 27.903933 12-13 ACGCAGA 27055 0.0 26.852194 7 CGCAGAG 27595 0.0 26.507261 8 GTACTTT 16500 0.0 25.915648 14-15 GCAGAGT 28090 0.0 25.193321 9 CATGGGG 7340 0.0 24.526608 4 AGAGTAC 23400 0.0 24.398855 10-11 AGTACTT 16860 0.0 24.127518 12-13 CAGAGTA 27725 0.0 22.051374 10-11 CATGGGA 9895 0.0 21.868422 4 >>END_MODULE