FastQCFastQC Report
Thu 26 May 2016
SRR1364475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4434497
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102450.051.7125971
GGTATCA58250.048.0308461
ATCAACG123400.041.0342453
TCAACGC124550.040.320494
CAACGCA126400.039.8703965
AACGCAG127100.038.738876
TATCAAC137450.038.094752
GTACATG167800.036.6460081
TACATGG175750.033.8218462
ACGCAGA150150.032.3168537
CGCAGAG147950.031.914598
ACATGGG175100.030.9234053
GCAGAGT163200.027.6918989
CATGGGA112200.023.9986924
AGAGTAC150100.022.5010610-11
CATGGGG87400.022.4246884
GAGTACT67550.022.19065312-13
CAGAGTA161150.022.10302210-11
GTATAGG16850.020.4902081
GAGTACA138000.019.4543081
Produced by FastQC (version 0.11.5)