FastQCFastQC Report
Thu 26 May 2016
SRR1364475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4434497
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77740.17530736856964838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70150.15819156039568863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75750.058.7863541
GTATCAA130500.051.504861
ATCAACG170050.037.9290283
TCAACGC171950.037.2672544
CAACGCA171450.037.096895
TATCAAC181150.037.052812
AACGCAG174750.035.987786
GTACATG167800.033.2257841
TACATGG177200.030.847982
ACGCAGA210150.029.6428137
CGCAGAG213900.029.5257118
ACATGGG175350.028.3858433
GCAGAGT237600.025.7296149
GAGTACT128550.024.86610212-13
AGAGTAC217600.023.9002410-11
AGTACTT140700.023.26829312-13
CAGAGTA228100.022.2786110-11
GTACTTT144950.022.27847114-15
CATGGGG89350.021.7492434
CATGGGA110700.021.0547564
Produced by FastQC (version 0.11.5)