##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364475_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4434497 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.978665900551967 31.0 28.0 31.0 25.0 34.0 2 29.201601444312626 31.0 28.0 34.0 25.0 34.0 3 29.290314324262706 31.0 28.0 34.0 25.0 34.0 4 32.71760246990809 35.0 32.0 37.0 26.0 37.0 5 31.835487316825336 35.0 32.0 37.0 22.0 37.0 6 31.867764934782908 35.0 32.0 37.0 22.0 37.0 7 31.33758349594103 35.0 32.0 37.0 17.0 37.0 8 31.440730030936994 35.0 32.0 37.0 17.0 37.0 9 32.04630175643371 37.0 31.0 39.0 11.0 39.0 10-11 32.23505236332328 37.0 31.5 39.0 16.5 39.0 12-13 31.97875598968722 37.0 30.5 39.0 13.5 39.0 14-15 32.93654195729527 38.0 31.0 40.0 13.5 41.0 16-17 32.994302623273846 38.0 31.5 40.0 14.5 41.0 18-19 32.83765216212797 38.0 31.0 40.0 11.0 41.0 20-21 32.71925237518483 38.0 31.0 40.0 10.5 41.0 22-23 32.68346646756103 38.0 31.0 40.0 10.5 41.0 24-25 32.47375113795319 38.0 30.5 40.0 10.0 41.0 26-27 32.56535363537285 37.5 31.0 40.0 10.0 41.0 28-29 32.45126764095229 37.0 30.5 40.0 9.5 41.0 30-31 31.93868729643971 37.0 30.0 40.0 8.0 41.0 32-33 31.895473601628325 37.0 30.0 40.0 8.0 41.0 34-35 31.700193054589956 37.0 30.0 40.0 7.5 41.0 36-37 31.694783421885276 37.0 30.0 40.0 2.0 41.0 38-39 31.462205521843856 37.0 30.0 40.0 2.0 41.0 40-41 31.188482030769215 36.5 29.5 40.0 2.0 41.0 42-43 31.078556260157576 36.0 29.5 40.0 2.0 41.0 44-45 31.004737628641983 36.0 29.0 40.0 2.0 41.0 46-47 30.86210341330708 36.0 29.0 40.0 2.0 41.0 48-49 30.600830376026863 36.0 28.0 40.0 2.0 41.0 50-51 30.247703741822352 36.0 28.0 40.0 2.0 41.0 52-53 30.144054105798244 36.0 28.0 40.0 2.0 41.0 54-55 29.667011162709095 35.0 26.5 39.5 2.0 41.0 56-57 29.675724552299844 35.0 27.0 39.0 2.0 41.0 58-59 29.42360486431719 35.0 26.5 39.0 2.0 41.0 60-61 29.126036165995828 35.0 26.0 39.0 2.0 41.0 62-63 28.8451023870351 34.0 26.0 39.0 2.0 40.5 64-65 28.358148849801907 34.0 25.0 38.0 2.0 40.0 66-67 27.956084421750653 34.0 23.5 38.0 2.0 40.0 68-69 27.76778854512699 34.0 23.5 37.0 2.0 40.0 70-71 27.843592294684157 34.0 25.5 37.0 2.0 39.5 72-73 27.618805920942105 34.0 24.5 36.5 2.0 39.0 74-75 27.25248342709444 34.0 23.5 36.0 2.0 39.0 76-77 27.17908705316522 33.5 24.5 36.0 2.0 38.5 78-79 26.78688484849578 33.0 23.0 35.0 2.0 37.0 80-81 26.563363556227458 33.0 23.0 35.0 2.0 37.0 82-83 26.438903104455818 33.0 23.0 35.0 2.0 36.5 84-85 26.019775749087213 33.0 20.5 35.0 2.0 36.0 86-87 25.900663367231957 33.0 20.5 35.0 2.0 36.0 88-89 25.873653201253717 33.0 21.0 35.0 2.0 35.5 90-91 25.721877362866636 33.0 20.0 35.0 2.0 35.0 92-93 25.74913603504524 33.0 21.0 35.0 2.0 35.0 94-95 25.77070409563926 33.0 23.0 35.0 2.0 35.0 96-97 25.6578236494466 33.0 20.0 35.0 2.0 35.0 98-99 25.525409984492043 33.0 20.0 35.0 2.0 35.0 100-101 25.433637118257153 33.0 19.5 35.0 2.0 35.0 102-103 24.30391293533404 30.5 18.5 34.0 2.0 35.0 104-105 25.263258493578867 32.5 19.5 35.0 2.0 35.0 106-107 25.363472226951558 33.0 19.0 35.0 2.0 35.0 108-109 25.44144025804956 33.0 19.5 35.0 2.0 35.0 110-111 25.37950313192229 33.0 19.0 35.0 2.0 35.0 112-113 25.33213158110153 33.0 19.0 35.0 2.0 35.0 114-115 25.28210268267179 33.0 19.0 35.0 2.0 35.0 116-117 25.202964507586767 33.0 18.0 35.0 2.0 35.0 118-119 25.08804290542986 33.0 16.5 35.0 2.0 35.0 120-121 24.92275922162085 33.0 6.0 35.0 2.0 35.0 122-123 24.632305535441787 33.0 2.0 35.0 2.0 35.0 124-125 23.851268926329187 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7635156634753493 1101 2 0.7445153764378318 1101 3 0.7200777192620649 1101 4 0.802901885925948 1101 5 0.935412674833298 1101 6 1.0464272204374687 1101 7 1.400378309434636 1101 8 1.3119054086070534 1101 9 1.9023785844647705 1101 10-11 1.6038268490444807 1101 12-13 1.7386691709190778 1101 14-15 1.655752239706068 1101 16-17 1.5853813462476865 1101 18-19 1.7515793131657134 1101 20-21 1.929330321431756 1101 22-23 1.8668018618036797 1101 24-25 2.0444318838111926 1101 26-27 1.729124166428278 1101 28-29 1.7785939695299788 1101 30-31 2.172893746130505 1101 32-33 2.09008462620902 1101 34-35 2.0805834575622804 1101 36-37 1.8921422372489722 1101 38-39 2.0399634752134546 1101 40-41 2.222359433834999 1101 42-43 2.122992192032733 1101 44-45 2.05446368100732 1101 46-47 1.835081729290657 1101 48-49 1.883952522049782 1101 50-51 2.141730631509116 1101 52-53 2.0055526164017543 1101 54-55 2.3154571531877437 1101 56-57 2.102322141561981 1101 58-59 2.177975479653032 1101 60-61 2.208181227929419 1101 62-63 2.1535259738340464 1101 64-65 2.3070530532823312 1101 66-67 2.5019495833423377 1101 68-69 2.436140741021049 1101 70-71 2.040586095652497 1101 72-73 2.2203607778427283 1101 74-75 2.3457009133077875 1101 76-77 2.082805499585266 1101 78-79 2.176826695111309 1101 80-81 2.2744479729680407 1101 82-83 2.1512351151072515 1101 84-85 2.3737372128044996 1101 86-87 2.33449929387713 1101 88-89 2.170972578937132 1101 90-91 2.106368947154877 1101 92-93 1.960371399740076 1101 94-95 1.9320298823818476 1101 96-97 1.8287933069335196 1101 98-99 1.912597291299786 1101 100-101 2.0409323928311416 1101 102-103 1.8944016237609134 1101 104-105 1.889371722048221 1101 106-107 2.028081546814981 1101 108-109 2.007227981628475 1101 110-111 1.9901711374904565 1101 112-113 1.9132370627659974 1101 114-115 1.9146180146982132 1101 116-117 1.939557983152362 1101 118-119 1.8387708854071612 1101 120-121 1.7800442068750115 1101 122-123 1.9167052155645905 1101 124-125 1.7114474535498125 1102 1 0.3794176315076818 1102 2 -1.5878861990611703 1102 3 0.01641007728368038 1102 4 0.45902982036623996 1102 5 0.9037299978432891 1102 6 0.4929284768351998 1102 7 1.2009477959758996 1102 8 0.9880414278293088 1102 9 1.7336664460247562 1102 10-11 1.4084947138753279 1102 12-13 1.541316926468273 1102 14-15 1.541779561089058 1102 16-17 1.4847784444917949 1102 18-19 1.5955205376528951 1102 20-21 1.7527657446470641 1102 22-23 1.5878463012339736 1102 24-25 1.8264394327011146 1102 26-27 1.548900293559555 1102 28-29 1.5979695274784405 1102 30-31 1.9297212656214455 1102 32-33 1.5669445320727249 1102 34-35 1.7471749753242314 1102 36-37 1.136511236805081 1102 38-39 1.148928385209615 1102 40-41 1.3080207275342595 1102 42-43 1.2350042814941347 1102 44-45 1.2545718211881365 1102 46-47 0.8486146064830038 1102 48-49 1.0127359572485837 1102 50-51 1.1094234572079067 1102 52-53 0.9972961076903637 1102 54-55 1.300472697811447 1102 56-57 0.8323095642970735 1102 58-59 0.8455098177249027 1102 60-61 0.9641973796921555 1102 62-63 1.237890827650972 1102 64-65 1.3373226756568712 1102 66-67 1.4731953935747129 1102 68-69 1.4114986389979869 1102 70-71 0.8061698545800375 1102 72-73 1.1353321016703042 1102 74-75 1.3497880076613598 1102 76-77 1.2561656178189828 1102 78-79 1.1273498521313456 1102 80-81 1.2132322298904832 1102 82-83 0.8106894695191045 1102 84-85 0.8324187293840595 1102 86-87 0.9381036487930672 1102 88-89 1.0569169053888672 1102 90-91 0.8735734273035263 1102 92-93 0.910101285512475 1102 94-95 0.9608427380710225 1102 96-97 1.053999114326139 1102 98-99 0.7615869593061397 1102 100-101 0.680046071083396 1102 102-103 0.5063105337550482 1102 104-105 0.7945857631285804 1102 106-107 1.1076162221816261 1102 108-109 0.9381055318560172 1102 110-111 0.858181261142569 1102 112-113 0.8582799600724336 1102 114-115 1.0881116946605651 1102 116-117 0.7140657536081676 1102 118-119 0.3889493782926081 1102 120-121 0.6536961614404433 1102 122-123 0.9833008018709464 1102 124-125 0.9866968844281523 1103 1 0.7404252921599657 1103 2 0.6333008040653922 1103 3 0.6363692920771165 1103 4 0.5871162677883959 1103 5 0.9366603680308181 1103 6 0.9618891894120516 1103 7 1.316842966806476 1103 8 1.1167259284730306 1103 9 1.8975790880597572 1103 10-11 1.5381575053286056 1103 12-13 1.573926232948498 1103 14-15 1.4760731754227265 1103 16-17 1.4520246667347436 1103 18-19 1.5004181118257733 1103 20-21 1.7064417826022265 1103 22-23 1.70767906934271 1103 24-25 2.0291317999230714 1103 26-27 1.576066785650596 1103 28-29 1.726348359760678 1103 30-31 2.2196691417110017 1103 32-33 2.0045565825779903 1103 34-35 2.1156858608157343 1103 36-37 1.9529565409954692 1103 38-39 2.0181067296895847 1103 40-41 2.1687476923853275 1103 42-43 2.1040735175320933 1103 44-45 1.9713741390134203 1103 46-47 1.8431927838273765 1103 48-49 1.8820368942971797 1103 50-51 1.9637018100866719 1103 52-53 1.893151682705163 1103 54-55 2.1901066630107486 1103 56-57 1.9224447419801685 1103 58-59 2.1156629114513272 1103 60-61 2.0414976434002803 1103 62-63 2.2178341976980853 1103 64-65 2.2755844878943456 1103 66-67 2.5108971594546716 1103 68-69 2.4986934534569336 1103 70-71 2.208671066092389 1103 72-73 2.336292196828328 1103 74-75 2.379317062342988 1103 76-77 2.265777910003724 1103 78-79 2.327316123864101 1103 80-81 2.330841964969526 1103 82-83 2.2227445701587314 1103 84-85 2.4800095172061454 1103 86-87 2.313389774674242 1103 88-89 2.260749362380679 1103 90-91 2.274606178357004 1103 92-93 2.0836197152514018 1103 94-95 1.9833852623286674 1103 96-97 1.918088864081657 1103 98-99 2.0771341594196393 1103 100-101 2.0654081868288117 1103 102-103 1.946309908226734 1103 104-105 1.934906463176283 1103 106-107 1.9312424415157174 1103 108-109 1.9468373538647867 1103 110-111 1.9744509675812019 1103 112-113 1.908232081391649 1103 114-115 1.6907183167753352 1103 116-117 1.811652246994619 1103 118-119 1.9093118877914215 1103 120-121 1.984948852854167 1103 122-123 2.138334151801267 1103 124-125 1.9621513179741434 1104 1 0.5187714806293897 1104 2 0.5241290377557739 1104 3 0.5037713047749257 1104 4 0.4806199400620912 1104 5 0.8116757578414315 1104 6 0.8461267285725746 1104 7 1.2895188950997252 1104 8 0.9596138430828418 1104 9 1.566679402786015 1104 10-11 1.1544672595793415 1104 12-13 1.278235480064037 1104 14-15 1.2889787245973565 1104 16-17 1.0142611008933429 1104 18-19 0.9581825128779755 1104 20-21 1.3663809163644416 1104 22-23 1.300786787601865 1104 24-25 1.5222201200607728 1104 26-27 1.4080870454211905 1104 28-29 1.4027575501639973 1104 30-31 1.8277091172635593 1104 32-33 1.8194665275251793 1104 34-35 1.7193468281200417 1104 36-37 1.5843971504469856 1104 38-39 1.731882510438929 1104 40-41 1.9399622120745228 1104 42-43 1.9480866233315695 1104 44-45 1.617306907748258 1104 46-47 1.6720671329713284 1104 48-49 1.458457781657593 1104 50-51 1.7893017222793866 1104 52-53 1.5435183692907017 1104 54-55 1.977503642411783 1104 56-57 1.881111050527668 1104 58-59 1.9245213034622246 1104 60-61 1.9005180352623654 1104 62-63 1.9294274444278692 1104 64-65 2.1852422387357393 1104 66-67 2.072089109424539 1104 68-69 2.163844312657517 1104 70-71 2.019610715301848 1104 72-73 2.144240499482681 1104 74-75 2.2414994381331717 1104 76-77 2.048966869037635 1104 78-79 2.0580073168866235 1104 80-81 2.0219793868900844 1104 82-83 2.0056178615692914 1104 84-85 2.27515335723103 1104 86-87 2.1209817407474567 1104 88-89 1.9326090673950418 1104 90-91 2.0691614551417565 1104 92-93 1.787916283987098 1104 94-95 1.7175752175066918 1104 96-97 1.8212407433610487 1104 98-99 1.9115801257370073 1104 100-101 1.951877942032933 1104 102-103 1.8673457739212296 1104 104-105 1.7859816869281602 1104 106-107 1.8451261387115423 1104 108-109 1.7795006858972329 1104 110-111 1.7890908610347402 1104 112-113 1.6780445585397707 1104 114-115 1.6366448965380194 1104 116-117 1.6406786565805973 1104 118-119 1.5682289598708117 1104 120-121 1.6013693075873974 1104 122-123 1.7952732464067154 1104 124-125 1.4928278998148379 1105 1 0.44754708254674114 1105 2 0.5741990911028871 1105 3 0.3463997277536315 1105 4 0.4885121159363095 1105 5 0.7627734251149256 1105 6 0.7962111427801624 1105 7 1.0834847905286438 1105 8 0.9552755916341695 1105 9 1.4874539971219818 1105 10-11 1.2341710847453982 1105 12-13 1.3728418214284552 1105 14-15 1.3133763228317505 1105 16-17 1.2445512336702507 1105 18-19 1.4172030254951054 1105 20-21 1.5999208220469967 1105 22-23 1.5762777842102622 1105 24-25 1.69133624694377 1105 26-27 1.4054160868453636 1105 28-29 1.4902413285982306 1105 30-31 1.8916549105154061 1105 32-33 1.7410905117566955 1105 34-35 1.831780547904831 1105 36-37 1.695543610333413 1105 38-39 1.7213122611563918 1105 40-41 1.8937240662180308 1105 42-43 1.8699612615978438 1105 44-45 1.7951327703560906 1105 46-47 1.6338132824088447 1105 48-49 1.815663547760046 1105 50-51 1.9163945704396745 1105 52-53 1.8093249788590704 1105 54-55 2.0013887304999685 1105 56-57 1.8161155217709783 1105 58-59 1.7178645248989213 1105 60-61 1.5847863130904152 1105 62-63 1.3802564334243002 1105 64-65 1.7691595888047758 1105 66-67 2.036712386108672 1105 68-69 2.0266869334711544 1105 70-71 1.892110878539313 1105 72-73 2.094292579047302 1105 74-75 2.113765911922279 1105 76-77 1.9368851164838397 1105 78-79 2.069631903608208 1105 80-81 1.9916771942198181 1105 82-83 1.8908863031898662 1105 84-85 2.183255678008649 1105 86-87 2.125658708682373 1105 88-89 1.9126759407652507 1105 90-91 1.8906590476540046 1105 92-93 1.7525937645936267 1105 94-95 1.6906171172093494 1105 96-97 1.753053631945697 1105 98-99 1.836759053682119 1105 100-101 1.91171187679668 1105 102-103 1.7218325538055694 1105 104-105 1.4510924456952736 1105 106-107 1.6434048336635563 1105 108-109 1.6433038898324526 1105 110-111 1.7519698129078947 1105 112-113 1.7940131794036667 1105 114-115 1.672877327317007 1105 116-117 1.6375375539107395 1105 118-119 1.7354169166897329 1105 120-121 1.770506466117066 1105 122-123 1.8915326207031171 1105 124-125 1.7503163720201123 1106 1 0.45763241492691975 1106 2 0.41896645440561997 1106 3 0.41776799221333505 1106 4 0.3309901913514892 1106 5 0.5137557014077139 1106 6 0.7082655012492118 1106 7 0.9251259429559155 1106 8 0.6049719429477705 1106 9 1.333682034419951 1106 10-11 0.9662241239169731 1106 12-13 0.918472266133481 1106 14-15 0.9361183235648554 1106 16-17 0.9476753337510075 1106 18-19 1.0387558097765393 1106 20-21 0.8639530408597338 1106 22-23 0.9150236942408014 1106 24-25 0.7861052507342023 1106 26-27 0.8015724942540885 1106 28-29 0.7856707348369625 1106 30-31 1.158392471306648 1106 32-33 0.996826047830595 1106 34-35 0.9284098342731859 1106 36-37 1.0221393878866927 1106 38-39 1.1228265724968267 1106 40-41 1.411169469517663 1106 42-43 1.3579253131591784 1106 44-45 1.3118619458681025 1106 46-47 1.1631387827367732 1106 48-49 1.1233708921493246 1106 50-51 1.4465156514339093 1106 52-53 1.0817556414371232 1106 54-55 1.5148041716478353 1106 56-57 1.2159929819108335 1106 58-59 1.2650560841092435 1106 60-61 1.475758301950865 1106 62-63 1.5914113962896508 1106 64-65 1.5495907321320601 1106 66-67 1.735861528625243 1106 68-69 1.572345241072533 1106 70-71 1.410931112109413 1106 72-73 1.3216134663847612 1106 74-75 1.61460161597196 1106 76-77 1.2358022909923392 1106 78-79 1.2358187928020712 1106 80-81 1.3566182797862893 1106 82-83 1.223079252091349 1106 84-85 1.4819162355465245 1106 86-87 1.5099165013595481 1106 88-89 1.2698632383643655 1106 90-91 1.4275073877862035 1106 92-93 1.2932650302194268 1106 94-95 1.1856881881138293 1106 96-97 1.1617779933830654 1106 98-99 1.1297748606496008 1106 100-101 0.9013742445187773 1106 102-103 0.7898582560120104 1106 104-105 0.9991306489258776 1106 106-107 0.9523240769133814 1106 108-109 0.9539328971045791 1106 110-111 0.9492491549705449 1106 112-113 0.7534848546008348 1106 114-115 0.8853978971535632 1106 116-117 1.0664824956289323 1106 118-119 0.8250622253713153 1106 120-121 0.8031011892481459 1106 122-123 1.0575713987443578 1106 124-125 0.9954497662204496 1107 1 0.3466104354512787 1107 2 0.40628282195163834 1107 3 0.2593027948613802 1107 4 0.28388702915840724 1107 5 0.2189926015556658 1107 6 0.5946225577679733 1107 7 0.7904233366005187 1107 8 0.7575064944868259 1107 9 1.4217801646698618 1107 10-11 0.8668502542601004 1107 12-13 1.114801901615838 1107 14-15 0.8853687832327353 1107 16-17 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-5.004660664376267 2216 82-83 -5.301958992663952 2216 84-85 -5.2555567714021265 2216 86-87 -5.694595515911402 2216 88-89 -5.179338252993922 2216 90-91 -4.684723763917958 2216 92-93 -4.299321102306209 2216 94-95 -4.06168393791447 2216 96-97 -4.29177886964283 2216 98-99 -4.102272708550419 2216 100-101 -4.486240060219508 2216 102-103 -4.4831181121942265 2216 104-105 -4.355414367584302 2216 106-107 -4.49239456665855 2216 108-109 -4.558802937554454 2216 110-111 -4.216184967394629 2216 112-113 -4.23676746959951 2216 114-115 -4.08161912327429 2216 116-117 -4.04399340945535 2216 118-119 -4.231640776292469 2216 120-121 -4.2041298098417315 2216 122-123 -4.200064767270035 2216 124-125 -4.131957512924128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 220095.0 3 73551.0 4 35803.0 5 28927.0 6 29033.0 7 33660.0 8 37982.0 9 39671.0 10 42566.0 11 45679.0 12 48787.0 13 51377.0 14 51901.0 15 42760.0 16 31624.0 17 27705.0 18 24478.0 19 22368.0 20 22580.0 21 24022.0 22 29912.0 23 41127.0 24 56545.0 25 75951.0 26 92563.0 27 105313.0 28 119335.0 29 135092.0 30 152281.0 31 172537.0 32 201352.0 33 240412.0 34 291721.0 35 371773.0 36 529911.0 37 665451.0 38 217945.0 39 707.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.739404859888026 19.63669354966145 14.09187834974561 24.532023240704916 2 15.894276739813865 20.818886891662455 38.20582918008706 25.081007188436622 3 19.006710343924013 24.938972785413995 29.533360829875406 26.520956040786587 4 13.130402624051804 17.102446053112246 36.04827087790925 33.7188804449267 5 13.659519895943104 38.187532881406845 33.78329041602689 14.369656806623162 6 32.20277217648733 36.87162503771569 16.932935302201333 13.992667483595648 7 28.107718385911625 32.37951569407993 20.71582907306368 18.796936846944764 8 26.176226976813833 35.69886280225243 19.27294121520434 18.851969005729398 9 25.896353070032518 15.871743740045376 19.735361192035985 38.49654199788612 10-11 25.115788830398916 26.665566949492426 27.07884036289765 21.13980385721101 12-13 25.835177783164866 23.853556508944127 27.67095315679985 22.640312551091167 14-15 22.80750970012705 25.100823591863573 25.510623798336578 26.581042909672796 16-17 22.577697113998465 28.498798631627388 26.017289917266883 22.90621433710727 18-19 22.538119842072362 26.983262358505115 28.306192715375754 22.172425084046765 20-21 23.264850680786108 26.126301456422453 28.298453057138584 22.31039480565286 22-23 22.913771233023446 25.971585944062646 28.17492685071909 22.939715972194815 24-25 22.78027241574381 26.263762495381165 28.11166887314617 22.84429621572886 26-27 22.659787579079403 26.56841235015422 28.05782141028061 22.713978660485772 28-29 22.545129520732246 26.407681580245846 28.237770242660254 22.809418656361657 30-31 22.489761728840087 26.218081123436665 28.2202168942834 23.071940253439845 32-33 22.502300430793085 26.37946075263804 28.023774068549624 23.09446474801925 34-35 22.929024939986853 26.386548936789154 27.84593111426571 22.83849500895828 36-37 22.95830632415591 26.156177897617276 27.842384945414306 23.043130832812505 38-39 22.75148281263319 26.354646476992333 27.99645189354869 22.89741881682579 40-41 22.75462990107423 26.163992444585965 28.027036583682797 23.05434107065701 42-43 22.897039144771114 26.236057496941008 27.700316295415316 23.166587062872566 44-45 22.946189915319422 26.40770660664541 27.472849379254917 23.173254098780248 46-47 22.985008860102386 26.630763971292275 27.05092540601098 23.333301762594356 48-49 23.09776232668684 26.684236500913467 26.884225473726488 23.333775698673207 50-51 23.06656316472307 26.69923774473145 26.739483487401888 23.49471560314359 52-53 23.34558877418456 26.62303221585872 26.63770131106307 23.39367769889365 54-55 23.050484093427908 26.541509464638462 26.73903755777372 23.66896888415991 56-57 22.992979689549454 26.92353270347518 26.709381833155355 23.374105773820013 58-59 23.077605662514973 26.607668605406342 26.725776728629363 23.588949003449322 60-61 23.172008009025603 26.50179941490546 26.966869072185638 23.359323503883303 62-63 23.134755809765895 26.60051149257195 26.87550471048082 23.38922798718134 64-65 23.06900383008624 26.449031241355 26.77409441047495 23.70787051808381 66-67 22.824406537362222 26.777804501859602 26.59979729809003 23.797991662688137 68-69 22.817225188542427 26.585818686712308 26.869238386230602 23.72771773851466 70-71 22.795178043935312 26.61271026882432 26.86824751042028 23.72386417682009 72-73 23.018741678514207 26.486854873287747 26.67251145262113 23.82189199557692 74-75 22.830990759377343 26.738325186475652 26.60418675191268 23.826497302234326 76-77 22.969896135833558 26.57367770931677 26.67759261545678 23.778833539392892 78-79 22.969459769289376 26.64615594858369 26.653575166771738 23.7308091153552 80-81 22.71782405397696 26.723001656336642 26.721738826492818 23.837435463193586 82-83 22.83576236508292 26.739915340751107 26.660075142919702 23.764247151246266 84-85 22.710269667624008 26.555937811196586 26.574237523272092 24.159554997907314 86-87 22.935760369287113 26.423185078780197 26.70444628356361 23.93660826836908 88-89 22.792694625740285 26.478407390886865 26.65756216013137 24.071335823241476 90-91 22.699171650153264 26.638078565712004 26.793925509625822 23.86882427450891 92-93 22.87954323991969 26.495823639156292 26.725044837330636 23.89958828359338 94-95 22.734679384707118 26.368686585653112 26.788822578464565 24.107811451175202 96-97 22.842642512957003 26.362234609029112 26.62070380791892 24.174419070094956 98-99 22.53320951620894 26.290039208505494 26.681041840822083 24.495709434463482 100-101 22.50872743554394 26.280650603667592 26.88971347085779 24.32090848993068 102-103 22.709538579218904 26.152232731392548 26.819127311399832 24.319101377988712 104-105 22.644895201578446 26.084017111778028 26.95571835269877 24.31536933394475 106-107 22.28267132229096 26.216864024816367 26.98538480047212 24.51507985242056 108-109 22.54901739701256 26.195406153166868 26.624440156346928 24.631136293473645 110-111 22.335603681100157 26.17355391189899 26.878550507335735 24.612291899665113 112-113 22.467135491188962 26.164185549876596 26.828939935663477 24.539739023270972 114-115 22.300082895702957 26.22788696237311 26.703928390380128 24.76810175154381 116-117 22.110839177476045 26.19760482417735 26.85982198206471 24.831734016281892 118-119 22.208128066042736 26.201679031188004 26.61028553181874 24.979907370950524 120-121 22.047293635797192 26.170800389050918 26.829922823501185 24.9519831516507 122-123 22.116060266024906 26.110921435517014 26.734186166921337 25.038832131536743 124-125 21.947481627026523 26.08701192164247 26.718991466821524 25.246514984509478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 173.0 1 158.5 2 231.5 3 348.5 4 294.0 5 261.0 6 495.0 7 1164.5 8 1899.0 9 2244.5 10 2033.5 11 1661.5 12 1664.5 13 1881.5 14 2165.0 15 2531.0 16 2967.5 17 3420.0 18 3908.0 19 4471.5 20 5070.0 21 5963.0 22 7283.5 23 8905.0 24 11051.5 25 14289.5 26 18780.0 27 24354.5 28 30559.0 29 37054.0 30 44362.5 31 52940.0 32 61056.5 33 67822.5 34 76724.0 35 84720.0 36 92452.5 37 101703.5 38 108197.5 39 112218.0 40 117115.5 41 125103.5 42 132272.5 43 141219.5 44 150859.0 45 156881.0 46 161408.0 47 165250.5 48 172897.0 49 179114.5 50 179119.5 51 177166.0 52 174302.5 53 171568.5 54 165970.5 55 154973.0 56 143231.0 57 131458.0 58 120380.5 59 108995.0 60 93787.5 61 76223.0 62 59545.0 63 44882.5 64 33209.5 65 25068.5 66 18592.0 67 14151.5 68 10534.0 69 7579.0 70 5686.0 71 4218.0 72 3202.0 73 2680.0 74 2167.5 75 1629.0 76 1100.0 77 619.0 78 363.5 79 207.0 80 123.5 81 69.5 82 47.5 83 44.0 84 27.5 85 19.0 86 18.0 87 11.5 88 4.5 89 2.5 90 1.5 91 1.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10560385991917459 2 3.1570660663430373E-4 3 0.0 4 0.18175680353374915 5 0.0 6 6.765141570735079E-5 7 0.03786224232421399 8 0.0 9 0.0 10-11 5.299360897075813E-4 12-13 4.961103818539058E-4 14-15 4.2845896614655506E-4 16-17 0.004025259234587373 18-19 0.0013755787860494663 20-21 0.0031119651225381363 22-23 0.0016236339769764192 24-25 0.004701773391660881 26-27 0.00423948871766065 28-29 0.0033600203134650894 30-31 7.328903368296337E-4 32-33 3.3825707853675396E-5 34-35 0.0011726245389274137 36-37 0.0010711474153663877 38-39 4.2845896614655506E-4 40-41 0.0013755787860494663 42-43 7.892665165857594E-4 44-45 0.0015334320893666182 46-47 2.480551909269529E-4 48-49 5.6376179756125665E-5 50-51 0.03901231639123896 52-53 9.020188760980107E-5 54-55 0.13559598754943344 56-57 1.465780673659267E-4 58-59 2.255047190245027E-5 60-61 0.0 62-63 5.412113256588063E-4 64-65 0.061855944428421086 66-67 0.053512269824514486 68-69 0.0018942396398058223 70-71 0.0025707537968793306 72-73 0.0720938586721335 74-75 0.006133728357466473 76-77 0.0022437719542938014 78-79 0.001522156853415393 80-81 2.7060566282940317E-4 82-83 3.1570660663430373E-4 84-85 2.255047190245027E-5 86-87 1.5785330331715187E-4 88-89 0.0014206797298543668 90-91 4.059084942441048E-4 92-93 0.0030217632349283357 94-95 0.004927278110685383 96-97 0.0026947813923428065 98-99 0.0 100-101 0.0013868540220006913 102-103 0.0014206797298543668 104-105 1.69128539268377E-4 106-107 9.020188760980107E-5 108-109 0.0 110-111 1.69128539268377E-4 112-113 4.510094380490054E-5 114-115 2.029542471220524E-4 116-117 0.0 118-119 7.892665165857594E-4 120-121 0.002615854740684231 122-123 5.637617975612566E-4 124-125 0.007543132851369615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4434497.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.14261276085242 #Duplication Level Percentage of deduplicated Percentage of total 1 75.75550220096635 37.98578811366959 2 11.44700373841233 11.479653514544792 3 4.655061186250976 7.00250791320771 4 2.416864256845924 4.847515541062822 5 1.4190886897129429 3.5578407320790775 6 0.9306020439094712 2.7997690753326245 7 0.6461567106411438 2.2679990007153528 8 0.4451569849793425 1.7857067452486215 9 0.3461680534294276 1.5621993587960876 >10 1.7555471210568663 15.724373440389696 >50 0.11890283551602851 4.0785944960093845 >100 0.061053703537869666 5.430386147185247 >500 0.0023874395649611598 0.8027870972004728 >1k 4.1321059894890795E-4 0.3344120690956188 >5k 9.182457754417899E-5 0.3404667554629158 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7774 0.17530736856964838 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7015 0.15819156039568863 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.2550471902450265E-5 0.0 0.0 0.0 0.0 3 2.2550471902450265E-5 0.0 0.0 0.0 0.0 4 2.2550471902450265E-5 0.0 0.0 0.0 0.0 5 2.2550471902450265E-5 0.0 0.0 0.0 0.0 6 1.1275235951225133E-4 0.0 0.0 0.0 0.0 7 1.8040377521960212E-4 0.0 0.0 0.0 0.0 8 1.8040377521960212E-4 0.0 0.0 0.0 2.2550471902450265E-5 9 1.8040377521960212E-4 0.0 0.0 0.0 2.2550471902450265E-5 10-11 2.029542471220524E-4 0.0 0.0 0.0 2.2550471902450265E-5 12-13 2.480551909269529E-4 0.0 0.0 1.1275235951225133E-5 2.2550471902450265E-5 14-15 2.931561347318535E-4 0.0 0.0 2.2550471902450265E-5 2.2550471902450265E-5 16-17 3.044313706830786E-4 0.0 0.0 9.020188760980107E-5 3.3825707853675396E-5 18-19 3.495323144879791E-4 0.0 0.0 1.8040377521960215E-4 4.510094380490053E-5 20-21 3.8335802234165455E-4 0.0 0.0 3.3825707853675404E-4 6.76514157073508E-5 22-23 3.8335802234165455E-4 0.0 0.0 6.314132132686075E-4 6.76514157073508E-5 24-25 4.059084942441048E-4 0.0 0.0 9.922207637078117E-4 6.76514157073508E-5 26-27 4.059084942441048E-4 0.0 0.0 0.001183899774878639 6.76514157073508E-5 28-29 4.397342020977802E-4 0.0 0.0 0.0019167901117082727 6.76514157073508E-5 30-31 5.186608537563561E-4 0.0 0.0 0.004092910650294723 6.76514157073508E-5 32-33 5.186608537563561E-4 0.0 0.0 0.008760858334101928 6.76514157073508E-5 34-35 5.524865616100315E-4 0.0 0.0 0.01531177042176373 6.76514157073508E-5 36-37 6.539636851710577E-4 0.0 0.0 0.027184593878403798 6.76514157073508E-5 38-39 7.441655727808588E-4 0.0 0.0 0.04654417400665735 6.76514157073508E-5 40-41 7.441655727808588E-4 0.0 0.0 0.0705266008749132 6.76514157073508E-5 42-43 8.456426963418849E-4 0.0 0.0 0.0962792397875114 6.76514157073508E-5 44-45 9.020188760980106E-4 0.0 0.0 0.12649687213679478 6.76514157073508E-5 46-47 9.245693480004609E-4 0.0 0.0 0.16407723356222814 6.76514157073508E-5 48-49 9.471198199029111E-4 0.0 0.0 0.2053220466718097 6.76514157073508E-5 50-51 9.471198199029111E-4 0.0 0.0 0.250062182926271 6.76514157073508E-5 52-53 9.471198199029111E-4 0.0 0.0 0.29890650506697825 6.76514157073508E-5 54-55 9.471198199029111E-4 0.0 0.0 0.3499156725103208 6.76514157073508E-5 56-57 9.922207637078117E-4 0.0 0.0 0.4118054426465956 6.76514157073508E-5 58-59 0.001014771235610262 0.0 0.0 0.4797951154324831 6.76514157073508E-5 60-61 0.0010373217075127122 0.0 0.0 0.558969822281986 6.76514157073508E-5 62-63 0.0010373217075127122 0.0 0.0 0.637806272052952 9.020188760980106E-5 64-65 0.0010373217075127122 0.0 0.0 0.7184580348120655 9.020188760980106E-5 66-67 0.0011275235951225134 0.0 0.0 0.8123694750498196 9.020188760980106E-5 68-69 0.0011275235951225134 0.0 0.0 0.92216772274285 9.020188760980106E-5 70-71 0.0011500740670249638 0.0 0.0 1.0529379093051592 9.020188760980106E-5 72-73 0.001172624538927414 0.0 0.0 1.1856023355072738 9.020188760980106E-5 74-75 0.001172624538927414 0.0 0.0 1.3241975358197333 9.020188760980106E-5 76-77 0.001183899774878639 2.2550471902450265E-5 1.1275235951225133E-5 1.4769657077228828 9.020188760980106E-5 78-79 0.001195175010829864 2.2550471902450265E-5 2.2550471902450265E-5 1.6527917371462872 9.020188760980106E-5 80-81 0.0013079273703421154 2.2550471902450265E-5 2.2550471902450265E-5 1.855249873886486 9.020188760980106E-5 82-83 0.0013304778422445658 2.2550471902450265E-5 2.2550471902450265E-5 2.0674723649604454 9.020188760980106E-5 84-85 0.0013530283141470162 2.2550471902450265E-5 2.2550471902450265E-5 2.279976736933185 9.020188760980106E-5 86-87 0.0013868540220006915 2.2550471902450265E-5 2.2550471902450265E-5 2.5106455140233495 9.020188760980106E-5 88-89 0.0013981292579519165 2.2550471902450265E-5 2.2550471902450265E-5 2.7708892350135765 9.020188760980106E-5 90-91 0.0015108816174641678 2.2550471902450265E-5 2.2550471902450265E-5 3.069299629698701 9.020188760980106E-5 92-93 0.0015108816174641678 4.510094380490053E-5 2.2550471902450265E-5 3.3840140155692966 9.020188760980106E-5 94-95 0.0015108816174641678 4.510094380490053E-5 2.2550471902450265E-5 3.7053356897073106 9.020188760980106E-5 96-97 0.0015108816174641678 4.510094380490053E-5 2.2550471902450265E-5 4.038699315841233 9.020188760980106E-5 98-99 0.001612358741025194 4.510094380490053E-5 2.2550471902450265E-5 4.401592784931414 9.020188760980106E-5 100-101 0.001702560628634995 4.510094380490053E-5 2.2550471902450265E-5 4.818178927621329 9.020188760980106E-5 102-103 0.001781487280293571 4.510094380490053E-5 2.2550471902450265E-5 5.262141343200819 9.020188760980106E-5 104-105 0.002029542471220524 4.510094380490053E-5 2.2550471902450265E-5 5.706577318690259 9.020188760980106E-5 106-107 0.0020859186509766493 4.510094380490053E-5 2.2550471902450265E-5 6.161093355120096 9.020188760980106E-5 108-109 0.002119744358830325 4.510094380490053E-5 2.2550471902450265E-5 6.637291670284139 9.020188760980106E-5 110-111 0.0021422948307327754 4.510094380490053E-5 2.2550471902450265E-5 7.160778325027619 9.020188760980106E-5 112-113 0.0021422948307327754 5.6376179756125665E-5 2.2550471902450265E-5 7.730189015800439 9.020188760980106E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7575 0.0 58.786354 1 GTATCAA 13050 0.0 51.50486 1 ATCAACG 17005 0.0 37.929028 3 TCAACGC 17195 0.0 37.267254 4 CAACGCA 17145 0.0 37.09689 5 TATCAAC 18115 0.0 37.05281 2 AACGCAG 17475 0.0 35.98778 6 GTACATG 16780 0.0 33.225784 1 TACATGG 17720 0.0 30.84798 2 ACGCAGA 21015 0.0 29.642813 7 CGCAGAG 21390 0.0 29.525711 8 ACATGGG 17535 0.0 28.385843 3 GCAGAGT 23760 0.0 25.729614 9 GAGTACT 12855 0.0 24.866102 12-13 AGAGTAC 21760 0.0 23.90024 10-11 AGTACTT 14070 0.0 23.268293 12-13 CAGAGTA 22810 0.0 22.27861 10-11 GTACTTT 14495 0.0 22.278471 14-15 CATGGGG 8935 0.0 21.749243 4 CATGGGA 11070 0.0 21.054756 4 >>END_MODULE