FastQCFastQC Report
Thu 26 May 2016
SRR1364474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2747741
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31430.11438487106317516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72300.050.471051
GGTATCA43850.049.5499731
ATCAACG84900.040.4358753
TATCAAC92650.039.750622
CAACGCA88400.039.5547685
TCAACGC87600.039.4407164
AACGCAG89500.038.250426
GTACATG123850.036.5289961
TACATGG125550.034.3573762
CGCAGAG104750.031.8883368
ACATGGG125100.031.7224863
ACGCAGA107050.031.4245417
GCAGAGT115900.026.920239
CATGGGA81600.026.2074764
GAGTACT52900.023.21989612-13
CAGAGTA116950.022.441910-11
AGAGTAC109800.022.19590610-11
CATGGGG52750.021.6820164
CGCATAG3108.385541E-921.1281729
GAGTACA99900.020.617251
Produced by FastQC (version 0.11.5)