##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364474_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2747741 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.23673373873302 30.0 25.0 31.0 2.0 34.0 2 25.527756800950307 30.0 25.0 31.0 2.0 34.0 3 25.46198640992728 30.0 25.0 31.0 2.0 34.0 4 28.13155570339417 35.0 26.0 37.0 2.0 37.0 5 28.777603857132096 35.0 27.0 37.0 2.0 37.0 6 28.86408435147272 35.0 28.0 37.0 2.0 37.0 7 28.659435514482624 35.0 27.0 37.0 2.0 37.0 8 28.285973095717537 35.0 26.0 37.0 2.0 37.0 9 28.818260163530695 35.0 25.0 39.0 2.0 39.0 10-11 28.890363575023994 35.0 26.0 39.0 2.0 39.0 12-13 28.733988392646907 35.0 25.5 39.0 2.0 39.0 14-15 29.32340748272854 36.0 25.0 40.0 2.0 41.0 16-17 29.5566903867577 36.0 26.5 40.0 2.0 41.0 18-19 29.50730054251838 36.0 26.0 40.0 2.0 41.0 20-21 29.29244404767407 36.0 24.5 40.0 2.0 41.0 22-23 29.329542704352413 36.0 25.0 40.0 2.0 41.0 24-25 29.344427113035763 36.0 25.0 40.0 2.0 41.0 26-27 29.43551484655941 36.0 25.5 40.0 2.0 41.0 28-29 29.36024392400885 36.0 25.0 40.0 2.0 41.0 30-31 29.00268420495236 35.0 24.5 40.0 2.0 41.0 32-33 28.99931652946912 35.0 25.0 40.0 2.0 41.0 34-35 28.70700750179875 35.0 24.0 40.0 2.0 41.0 36-37 28.468862057959612 35.0 23.5 40.0 2.0 41.0 38-39 28.315656388284047 35.0 23.0 39.5 2.0 41.0 40-41 28.32938020723205 35.0 23.0 40.0 2.0 41.0 42-43 28.190136916106724 35.0 23.0 39.0 2.0 41.0 44-45 27.833441361467475 34.0 21.0 39.0 2.0 41.0 46-47 27.58525821756854 34.0 19.0 39.0 2.0 40.5 48-49 27.3287520548698 33.5 18.0 39.0 2.0 40.0 50-51 27.312324924365143 33.0 18.5 39.0 2.0 40.0 52-53 27.110809206544577 33.0 16.0 39.0 2.0 40.0 54-55 26.990958754846254 33.0 16.0 39.0 2.0 40.0 56-57 26.582396594147703 33.0 13.0 38.5 2.0 40.0 58-59 26.362600769140904 33.0 12.5 38.0 2.0 40.0 60-61 25.963035999390044 32.5 8.5 38.0 2.0 40.0 62-63 25.914387491397477 32.0 8.0 38.0 2.0 40.0 64-65 25.73780298070306 32.0 8.0 37.5 2.0 40.0 66-67 25.497569457965653 32.0 8.0 37.0 2.0 40.0 68-69 25.322778238560332 32.0 7.0 36.5 2.0 40.0 70-71 25.188757237308756 32.0 7.0 36.0 2.0 39.0 72-73 25.166352469173766 32.0 4.0 36.0 2.0 39.0 74-75 24.905399198832786 32.0 2.0 36.0 2.0 39.0 76-77 24.62327144370594 32.0 2.0 35.0 2.0 38.5 78-79 24.310793666506413 31.5 2.0 35.0 2.0 37.0 80-81 24.31848070833459 32.0 2.0 35.0 2.0 37.0 82-83 24.125783325284296 32.0 2.0 35.0 2.0 36.5 84-85 23.949578945031575 31.5 2.0 35.0 2.0 36.0 86-87 23.747819208578974 31.0 2.0 35.0 2.0 36.0 88-89 23.520302677726903 31.0 2.0 35.0 2.0 35.5 90-91 23.35436873417109 31.0 2.0 35.0 2.0 35.0 92-93 23.252462477358677 31.0 2.0 35.0 2.0 35.0 94-95 23.184941011543664 31.0 2.0 35.0 2.0 35.0 96-97 23.051461728015852 31.0 2.0 35.0 2.0 35.0 98-99 22.956187828474373 31.0 2.0 35.0 2.0 35.0 100-101 22.8956488257081 31.0 2.0 35.0 2.0 35.0 102-103 21.819077744226988 29.0 2.0 33.5 2.0 34.5 104-105 22.54789443400961 30.5 2.0 34.0 2.0 35.0 106-107 22.633738587443283 31.0 2.0 34.5 2.0 35.0 108-109 22.57771347445047 31.0 2.0 35.0 2.0 35.0 110-111 22.470574555607676 31.0 2.0 35.0 2.0 35.0 112-113 22.447140214452524 31.0 2.0 35.0 2.0 35.0 114-115 22.398886758249777 31.0 2.0 35.0 2.0 35.0 116-117 22.204029055140204 31.0 2.0 35.0 2.0 35.0 118-119 22.053324712918723 31.0 2.0 35.0 2.0 35.0 120-121 21.906127797343345 31.0 2.0 35.0 2.0 35.0 122-123 21.634043201306092 30.5 2.0 34.0 2.0 35.0 124-125 20.706132237354247 28.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5807226521014464 1101 2 1.2311061216053147 1101 3 1.5431067673809622 1101 4 1.9702971859349034 1101 5 1.382467738007957 1101 6 1.1922610466717245 1101 7 1.0955997991094755 1101 8 1.590193981772952 1101 9 2.1446422836220087 1101 10-11 2.1146724347090036 1101 12-13 1.9369438940726837 1101 14-15 2.1846361968079435 1101 16-17 1.8555752667063317 1101 18-19 1.8248129256213055 1101 20-21 2.1408174735417838 1101 22-23 2.078935186255894 1101 24-25 1.8984244908146835 1101 26-27 1.6273800113298265 1101 28-29 1.4625431384882859 1101 30-31 1.637415262013807 1101 32-33 1.8620714559253777 1101 34-35 2.0923747563200337 1101 36-37 2.0674339490491285 1101 38-39 2.2209529647354564 1101 40-41 1.9929228645099677 1101 42-43 1.9869614763943986 1101 44-45 2.1344402965539224 1101 46-47 2.1829925852124106 1101 48-49 2.1184009658118157 1101 50-51 2.100256146413752 1101 52-53 1.8847830564348982 1101 54-55 1.9856304131541975 1101 56-57 2.0944039152969403 1101 58-59 2.0984217898907858 1101 60-61 2.2915728440258967 1101 62-63 1.953825050890547 1101 64-65 1.8736874826803351 1101 66-67 1.9978189473724903 1101 68-69 1.8193940108139408 1101 70-71 1.9184891547289418 1101 72-73 1.7533417961833813 1101 74-75 1.69073708611824 1101 76-77 1.9422191942628224 1101 78-79 1.9270412148408198 1101 80-81 1.7487332701186524 1101 82-83 1.7931190579833434 1101 84-85 1.6544100123570118 1101 86-87 1.6009755015341547 1101 88-89 1.7979117684447594 1101 90-91 1.7511385594407045 1101 92-93 1.7554403402247445 1101 94-95 1.6732413933570989 1101 96-97 1.7466478668812648 1101 98-99 1.678867288644927 1101 100-101 1.6708248675869228 1101 102-103 1.7223742433254827 1101 104-105 1.6273155501038374 1101 106-107 1.7340606782848447 1101 108-109 1.7580075391047174 1101 110-111 1.8641365237360574 1101 112-113 1.8365422775821756 1101 114-115 1.7511455052931808 1101 116-117 1.846778859285326 1101 118-119 1.8981534484805458 1101 120-121 1.8526030842653007 1101 122-123 1.8482082110517588 1101 124-125 1.8024317842752176 1102 1 0.7313739073037766 1102 2 0.41746393920927005 1102 3 0.5440556042086726 1102 4 1.0264341715766108 1102 5 0.9109717460409641 1102 6 0.9411604985044306 1102 7 0.8773425822868823 1102 8 0.9319272854997216 1102 9 1.2942103800537943 1102 10-11 0.8677474597006913 1102 12-13 1.288365098257195 1102 14-15 1.4674166030599203 1102 16-17 0.9694137820798971 1102 18-19 1.1483483371213161 1102 20-21 1.2384664605425435 1102 22-23 1.1271549244696715 1102 24-25 0.9945799961639317 1102 26-27 0.9231568179766789 1102 28-29 0.6902212839403319 1102 30-31 0.6929441203865387 1102 32-33 0.594802614514002 1102 34-35 0.9775413110703255 1102 36-37 1.16518604625497 1102 38-39 1.1190680990395627 1102 40-41 0.7915219368079462 1102 42-43 0.8002026436955525 1102 44-45 1.2035287599822375 1102 46-47 1.108950651796853 1102 48-49 1.2302224783682973 1102 50-51 1.0970216071480507 1102 52-53 1.1327686174391332 1102 54-55 0.8254572927919241 1102 56-57 1.0765540896813803 1102 58-59 1.09014348953934 1102 60-61 1.4907829864138478 1102 62-63 1.3277524818167414 1102 64-65 1.3513999610439136 1102 66-67 1.319190939564205 1102 68-69 1.122877329923643 1102 70-71 1.4468332026059656 1102 72-73 1.3248503397277709 1102 74-75 1.3297737848794888 1102 76-77 1.2955512256208124 1102 78-79 1.1842118886487327 1102 80-81 0.8240419542050397 1102 82-83 0.728971579847169 1102 84-85 0.7602556475738425 1102 86-87 0.8693944954817532 1102 88-89 0.6967208622966687 1102 90-91 0.5614502822127001 1102 92-93 0.5750407876227115 1102 94-95 0.7503292022306844 1102 96-97 0.8274882183967733 1102 98-99 0.9482813796744018 1102 100-101 0.8629773672458221 1102 102-103 0.676678642932039 1102 104-105 0.629922287558724 1102 106-107 0.8697805725573104 1102 108-109 0.9888662721043531 1102 110-111 0.7925990535990017 1102 112-113 0.7645969926502261 1102 114-115 0.8562487470334403 1102 116-117 1.0409687608613432 1102 118-119 1.1488747740341338 1102 120-121 1.097988763249905 1102 122-123 1.2448121956313791 1102 124-125 1.1798515857150598 1103 1 1.4125989278597473 1103 2 1.034623716241871 1103 3 1.2775705578185175 1103 4 1.7530945460968788 1103 5 1.2787972348249248 1103 6 1.2276897252582124 1103 7 1.25836188781674 1103 8 1.4899352842466165 1103 9 2.2442724029566747 1103 10-11 1.6755206122923418 1103 12-13 1.8450025139865858 1103 14-15 2.120779268311967 1103 16-17 1.8731710931502157 1103 18-19 1.5659085174192668 1103 20-21 2.019741129372857 1103 22-23 1.8499958873633133 1103 24-25 1.8928417740813366 1103 26-27 1.6391583164659878 1103 28-29 1.4936246007623275 1103 30-31 1.6368388802444152 1103 32-33 1.6790319297483194 1103 34-35 1.4944249968836658 1103 36-37 1.874377865976541 1103 38-39 1.965972727982642 1103 40-41 2.006740161801435 1103 42-43 1.9542104425403402 1103 44-45 1.7771531683185735 1103 46-47 2.0487164956477883 1103 48-49 2.0159949169921383 1103 50-51 1.9273124236760744 1103 52-53 1.9789684860495278 1103 54-55 1.9229979587832275 1103 56-57 2.0517260421060044 1103 58-59 2.179090805237802 1103 60-61 2.07260901122833 1103 62-63 1.988508224953403 1103 64-65 1.9273721375985247 1103 66-67 1.852334033090436 1103 68-69 1.769177720897563 1103 70-71 1.8720547256515054 1103 72-73 1.8668613993509595 1103 74-75 1.8744174066709078 1103 76-77 1.9402050311047425 1103 78-79 2.050863423990137 1103 80-81 1.6469903062439712 1103 82-83 1.7583981248065044 1103 84-85 1.7865704809761738 1103 86-87 1.756896447910819 1103 88-89 1.8286437209368138 1103 90-91 1.8014550379305412 1103 92-93 1.8266652141521362 1103 94-95 1.611503258841367 1103 96-97 1.7197336305212723 1103 98-99 1.5928176788806105 1103 100-101 1.46266490094186 1103 102-103 1.5720598114430828 1103 104-105 1.7215306475937417 1103 106-107 1.43930851292658 1103 108-109 1.8167815038465918 1103 110-111 1.9423245431816838 1103 112-113 1.839853260058458 1103 114-115 1.7273114131285787 1103 116-117 1.8141911573970226 1103 118-119 1.9073410500374912 1103 120-121 1.8863517202569717 1103 122-123 1.85133428492572 1103 124-125 1.818685654981028 1104 1 1.3204422828114915 1104 2 1.206094806849734 1104 3 1.2029723256875577 1104 4 1.4853724338136232 1104 5 1.1215625744648392 1104 6 1.11923143053669 1104 7 1.1370261724841555 1104 8 1.503044586008368 1104 9 1.988728966270898 1104 10-11 1.8159312989906766 1104 12-13 1.4059971395587212 1104 14-15 1.762244538497928 1104 16-17 1.5344762901687616 1104 18-19 0.894559202527919 1104 20-21 -3.6613961617212034 1104 22-23 -0.40556032355043925 1104 24-25 1.0185282442709145 1104 26-27 1.231569946669854 1104 28-29 0.7379630944632112 1104 30-31 1.5355338114938384 1104 32-33 1.3272399158381845 1104 34-35 1.7213244089327375 1104 36-37 1.890637670853625 1104 38-39 1.9134485983596576 1104 40-41 2.112161107834474 1104 42-43 1.9867508169860777 1104 44-45 2.157117401531611 1104 46-47 2.0898545037808205 1104 48-49 2.103619996660214 1104 50-51 2.049102728047547 1104 52-53 2.0587487440952437 1104 54-55 1.9222068766032052 1104 56-57 2.1962015880281456 1104 58-59 2.203262719851711 1104 60-61 2.2185833736156226 1104 62-63 1.7867904697858314 1104 64-65 2.0324081483049063 1104 66-67 1.9701260566732408 1104 68-69 1.9061622334866435 1104 70-71 2.0865405319597237 1104 72-73 2.0451626541271004 1104 74-75 2.0793629148633386 1104 76-77 1.9947564780199905 1104 78-79 1.95301417817425 1104 80-81 1.7204342317989259 1104 82-83 1.6274228613893058 1104 84-85 1.554242784284078 1104 86-87 1.551770365754976 1104 88-89 1.7593601459024661 1104 90-91 1.7653846396332682 1104 92-93 1.4325299463275698 1104 94-95 1.5149983033871486 1104 96-97 1.6161559845581053 1104 98-99 1.4938377561820353 1104 100-101 1.084702462849556 1104 102-103 1.4896668557937325 1104 104-105 1.5754438930277708 1104 106-107 1.8044478077345225 1104 108-109 1.7530381850832377 1104 110-111 1.7945832632481995 1104 112-113 1.8316946813180124 1104 114-115 1.6958422339237202 1104 116-117 1.788932250605992 1104 118-119 1.7134961619681164 1104 120-121 1.8006287914745016 1104 122-123 1.612991398782885 1104 124-125 1.720550986651503 1105 1 1.1780933856034608 1105 2 0.8656155133414352 1105 3 0.9021086409861034 1105 4 1.342397703571443 1105 5 0.8677894338587819 1105 6 1.026214169398692 1105 7 0.8167306460704147 1105 8 1.2193122227100304 1105 9 1.5303165636360063 1105 10-11 1.374168566270491 1105 12-13 1.466971940880807 1105 14-15 1.6010007295258113 1105 16-17 1.3247538305997146 1105 18-19 1.0904711981558712 1105 20-21 1.2254352641949104 1105 22-23 1.2306023340889354 1105 24-25 1.2150948826404004 1105 26-27 1.1752025969905553 1105 28-29 0.6690949653523077 1105 30-31 1.1197019701646163 1105 32-33 1.0544653234144086 1105 34-35 0.8306065721113463 1105 36-37 1.078125021716172 1105 38-39 0.9180598274525344 1105 40-41 1.0628133244953908 1105 42-43 1.312379836911223 1105 44-45 1.580263290697605 1105 46-47 1.8255743591438538 1105 48-49 1.3579227517301362 1105 50-51 1.3375687417438407 1105 52-53 1.329450287869932 1105 54-55 1.3390192588505059 1105 56-57 1.7111852162402315 1105 58-59 1.3923815301273095 1105 60-61 1.6868801926621444 1105 62-63 1.675455368889608 1105 64-65 1.4042650172095108 1105 66-67 1.5086234072442295 1105 68-69 1.0196016134996135 1105 70-71 1.1178947510096897 1105 72-73 1.0961139118264498 1105 74-75 1.357381219854446 1105 76-77 1.331509849048377 1105 78-79 1.1524995351815903 1105 80-81 1.079705275441352 1105 82-83 1.1177877034801895 1105 84-85 1.0952846465180244 1105 86-87 1.1862139111781218 1105 88-89 1.2564534778624399 1105 90-91 1.2755150957342956 1105 92-93 1.2096616412194123 1105 94-95 1.1500301467593879 1105 96-97 1.27372332372439 1105 98-99 1.0557763925161687 1105 100-101 1.200034770986484 1105 102-103 1.2258219957753909 1105 104-105 1.239672047182637 1105 106-107 1.359516087007048 1105 108-109 1.4261218261280106 1105 110-111 1.261137321112777 1105 112-113 1.3391525382758722 1105 114-115 1.216735044361485 1105 116-117 1.3795941386032133 1105 118-119 1.376383667163438 1105 120-121 1.2775489704426413 1105 122-123 1.3578666492916724 1105 124-125 1.0279962662966646 1106 1 -0.6306315303943784 1106 2 -0.46995237135855206 1106 3 -0.40144221001894564 1106 4 -0.8042915301197162 1106 5 -1.3833260139540542 1106 6 -0.7747612256230774 1106 7 -0.7563499179578841 1106 8 -0.3683361171042563 1106 9 -0.43434704401492397 1106 10-11 -2.9140367026650438 1106 12-13 -1.0326274982256258 1106 14-15 -0.7848899462712176 1106 16-17 -0.9999981788257095 1106 18-19 -3.7299008815366825 1106 20-21 -6.045605921707967 1106 22-23 -6.943271856103507 1106 24-25 -6.931170095539699 1106 26-27 -5.755265453214307 1106 28-29 -2.4364948034255924 1106 30-31 -4.126148614881213 1106 32-33 -4.6972514802504755 1106 34-35 -3.976608847814955 1106 36-37 -6.287108524077997 1106 38-39 -6.746763370894055 1106 40-41 -3.2023153367359747 1106 42-43 -1.7983879233960849 1106 44-45 -0.9885711978769578 1106 46-47 -0.8463565745394348 1106 48-49 -0.570405877612334 1106 50-51 -0.7387705227178252 1106 52-53 -0.908509718756978 1106 54-55 -0.8607405833384618 1106 56-57 -0.6208129050880196 1106 58-59 -0.4419266403887967 1106 60-61 -0.22537617454193182 1106 62-63 -0.5082572326770318 1106 64-65 -0.6914827656919016 1106 66-67 -1.0095832118783044 1106 68-69 -0.7970984503919105 1106 70-71 -0.5675840067251094 1106 72-73 -0.6158855407331068 1106 74-75 -0.6746620539368848 1106 76-77 -0.7454493207658821 1106 78-79 -0.7458934769047261 1106 80-81 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-4.361910567617155 2216 86-87 -4.650341024933525 2216 88-89 -4.916772963220719 2216 90-91 -4.945103768493926 2216 92-93 -4.637479113133814 2216 94-95 -7.585354181981128 2216 96-97 -9.524185885512884 2216 98-99 -6.505172288509449 2216 100-101 -5.538058511215134 2216 102-103 -6.473092734531992 2216 104-105 -8.97712809074458 2216 106-107 -5.862317930729461 2216 108-109 -8.077644764208648 2216 110-111 -5.646985225265176 2216 112-113 -5.064587865645052 2216 114-115 -5.3819662737133065 2216 116-117 -4.940551953978872 2216 118-119 -5.319092407771038 2216 120-121 -6.084953926769781 2216 122-123 -8.68699139649716 2216 124-125 -7.4397429867524565 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 330371.0 3 46022.0 4 26224.0 5 21722.0 6 21582.0 7 23550.0 8 25709.0 9 26804.0 10 28295.0 11 29018.0 12 28852.0 13 29458.0 14 29982.0 15 25774.0 16 21241.0 17 18970.0 18 17155.0 19 16212.0 20 16033.0 21 17862.0 22 22408.0 23 30200.0 24 40213.0 25 50611.0 26 60709.0 27 67593.0 28 76937.0 29 85504.0 30 95466.0 31 106918.0 32 124072.0 33 149420.0 34 180210.0 35 222746.0 36 280358.0 37 277969.0 38 75390.0 39 181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94804034749056 18.704994038366472 13.787456927061154 23.559508687081816 2 14.96443730411575 20.18731349016087 40.924022121427576 23.924227084295804 3 18.809471710080068 26.147545728704692 28.67326954731686 26.369713013898384 4 11.263999848527273 16.54635629807939 38.11960446684331 34.07003938655002 5 13.342009286717198 38.71305558630413 33.77311483719115 14.171820289787524 6 31.24896503407426 37.3652997534279 17.612818109856516 13.772917102641324 7 28.370294482122354 33.121677909382555 20.565417321000616 17.942610287494475 8 26.0768294600276 35.60979817272362 19.803318853322928 18.51005351392585 9 26.301034130604574 16.90720193680208 20.137304185219072 36.654459747374275 10-11 24.378652907631626 27.47365281476688 27.265872497067477 20.88182178053402 12-13 25.326336737731754 24.736663194573342 27.778238782481722 22.158761285213178 14-15 22.39196854884543 26.10228904583569 25.606601787043743 25.899140618275133 16-17 22.24641413488786 29.56665677797211 25.85974312447561 22.327185962664416 18-19 22.039794693046012 28.021756625866622 28.166407685215418 21.77204099587195 20-21 22.91109688697258 27.11423872100822 28.193949226384817 21.780715165634383 22-23 22.571442246812552 27.19521696425663 27.8679314611594 22.36540932777142 24-25 22.20553568721458 27.27569041397995 28.173651261569383 22.345122637236084 26-27 22.1446839299564 27.606426401485223 27.72875120605124 22.520138462507138 28-29 22.157425436281912 27.607974086947028 27.834549524138808 22.40005095263225 30-31 22.13948432133863 27.232180957197105 28.10150818894133 22.526826532522932 32-33 22.030944734474495 27.412334656180942 27.78143752675054 22.775283082594022 34-35 22.487372273547688 27.27446952446802 27.90415868210453 22.333999519879765 36-37 22.62201763877167 26.968548569947853 27.85039616813644 22.559037623144036 38-39 22.26218295261047 27.186027158293445 27.883737507317864 22.66805238177822 40-41 22.51727269368106 27.158916062817873 27.899334284347027 22.424476959154042 42-43 22.41080095784304 27.187276950072853 27.605050259825298 22.796871832258812 44-45 22.272441425705576 27.34295355956386 27.788058444222436 22.596546570508124 46-47 22.568388435504502 27.52280706351585 27.263095966634985 22.645708534344664 48-49 22.57523854170003 27.37628151560239 27.276555507350608 22.771924435346975 50-51 22.58309020286108 27.58555426468319 27.076454342426704 22.75490119002903 52-53 22.794231923440755 27.50471616219594 27.0814171744374 22.619634739925903 54-55 22.566987760485716 27.473584963688367 27.0860376508329 22.873389624993017 56-57 22.467212874605043 27.771931283564395 27.030814593262036 22.73004124856853 58-59 22.605155707100742 27.28381212505487 27.141950832095198 22.96908133574919 60-61 22.43856151711715 27.36175840785993 27.51835345666779 22.681326618355133 62-63 22.457486162348197 27.45789379438458 27.365084714672133 22.719535328595097 64-65 22.354034971444918 27.395790739101926 27.232319879515988 23.017854409937165 66-67 22.342241843672202 27.70334405800055 26.96681783109377 22.987596267233474 68-69 22.564824784869735 27.426867903185332 27.222433418214102 22.785873893730827 70-71 22.34876028967215 27.23221012222045 27.47674565029563 22.942283937811766 72-73 22.471823012598442 27.42558287395263 27.164493775023008 22.93810033842592 74-75 22.39037614540669 27.73629278738617 27.023664034903195 22.849667032303945 76-77 22.54167691817188 27.487730950034283 27.17749249880985 22.79309963298398 78-79 22.577548708930546 27.471329032286246 27.23040754262447 22.72071471615874 80-81 22.54712888482566 27.838524019190018 26.897238322685556 22.717108773298765 82-83 22.589113439537375 27.74583819433109 26.972136587986288 22.692911778145245 84-85 22.585987465138988 27.701617026913812 26.837220406762462 22.875175101184738 86-87 22.656850365645568 27.436674176653746 26.996975078379915 22.909500379320768 88-89 22.574446824892348 27.528906428137418 27.01127577712384 22.8853709698464 90-91 22.481533404375497 27.730464860332265 27.04091548311584 22.747086252176395 92-93 22.55889670312393 27.53765705908814 27.025336173049965 22.87811006473797 94-95 22.49132221667208 27.420903654086043 27.009210505963672 23.078563623278207 96-97 22.66505830200583 27.28316237634969 27.080667106182823 22.971112215461666 98-99 22.397692754081017 27.375744329376133 26.96310703002866 23.26345588651419 100-101 22.354086523588908 27.397413827615196 27.11854321266227 23.129956436133625 102-103 22.338817816871178 27.063969183194146 27.33120878516982 23.266004214764852 104-105 22.27446547317184 27.238366919406893 27.26156884311023 23.225598764311037 106-107 22.327135582012268 27.330634850677853 27.107667929411942 23.234561637897936 108-109 22.34525666058278 27.296679580521126 26.914941432728163 23.443122326167934 110-111 22.17217180786367 27.434768462343058 27.16905280611892 23.224006923674352 112-113 22.34476215051478 27.508065585965795 27.078422662727032 23.068749600792394 114-115 22.12125182427293 27.683328056134833 26.95509286049635 23.24032725909589 116-117 22.02414282243734 27.622995642572672 27.230326747907043 23.122534787082945 118-119 22.13861727745347 27.709479883587562 26.829435176202743 23.322467662756228 120-121 22.03908691527541 27.811152034535574 26.993130409036294 23.156630641152727 122-123 22.07074398043984 27.798324808398235 26.961584291311848 23.16934691985008 124-125 22.246313892514575 27.860805939502008 26.65611520693782 23.236764961045594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 999.0 1 1091.5 2 1266.5 3 1261.0 4 1011.0 5 803.5 6 748.5 7 877.5 8 1154.5 9 1288.0 10 1250.5 11 1222.0 12 1322.0 13 1482.5 14 1635.0 15 1845.5 16 2065.0 17 2246.0 18 2520.5 19 2910.5 20 3381.5 21 4163.0 22 5312.0 23 6714.5 24 8493.0 25 11032.5 26 14491.0 27 18649.5 28 22996.5 29 27792.5 30 33574.5 31 40535.0 32 47528.5 33 53102.0 34 57971.0 35 62706.0 36 68616.5 37 74828.0 38 78743.0 39 81291.0 40 83690.5 41 86784.0 42 88961.0 43 90976.5 44 94231.5 45 97102.0 46 97804.0 47 97060.5 48 97339.0 49 96730.0 50 94747.5 51 93147.0 52 92131.5 53 91681.0 54 90597.0 55 85046.0 56 77452.0 57 71133.0 58 65876.5 59 59397.5 60 50909.5 61 42893.0 62 35367.0 63 27472.0 64 20671.5 65 16071.0 66 12389.5 67 9661.0 68 7358.0 69 5509.0 70 4189.5 71 3133.0 72 2464.5 73 2020.5 74 1535.0 75 1139.5 76 813.0 77 535.0 78 379.5 79 250.0 80 162.5 81 114.0 82 92.5 83 68.5 84 45.5 85 46.5 86 33.0 87 17.5 88 16.0 89 14.0 90 13.5 91 9.0 92 6.0 93 7.0 94 5.0 95 3.0 96 3.0 97 3.0 98 2.0 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09782581400503178 2 0.03282696586031944 3 0.04228928417925853 4 0.04993192589840163 5 0.05287980199007112 6 0.002401973111730691 7 0.001310167151853104 8 0.0016377089398163802 9 0.004330830307514427 10-11 0.06594507997660624 12-13 0.004330830307514427 14-15 0.008825431509010493 16-17 0.0180511918699761 18-19 0.016013154078204604 20-21 0.13027064777939407 22-23 0.013265442412512679 24-25 0.012719539432573885 26-27 0.005022307415436899 28-29 0.00331181141162868 30-31 0.0015103315778306615 32-33 0.004840339755457302 34-35 0.018651685147908773 36-37 0.0016013154078204606 38-39 0.006641819589255319 40-41 0.030843518366541825 42-43 0.01157314317470242 44-45 0.11307470391132206 46-47 0.0026385310697041677 48-49 0.007751822315130865 50-51 0.008916415339000292 52-53 0.009971827766881958 54-55 0.007478870825161469 56-57 0.004749355925467502 58-59 0.01233740734661673 60-61 0.030825321600543867 62-63 0.006004932779326727 64-65 0.005695587757361411 66-67 0.0065872292912614394 68-69 0.0058775554173410086 70-71 0.003020663155661323 72-73 7.278706399183911E-4 74-75 9.826253638898282E-4 76-77 0.006441655163277761 78-79 0.021126445323631303 80-81 0.002292792515742932 82-83 0.003839517625569513 84-85 0.0016013154078204606 86-87 0.002420169877728651 88-89 0.004567388265487905 90-91 0.002802301963685806 92-93 0.0017832830678000582 94-95 0.0023109892817408917 96-97 8.188544699081901E-4 98-99 0.001801479833798018 100-101 0.0018560701317918973 102-103 7.096738739204314E-4 104-105 0.004512797967494025 106-107 8.734447679020693E-4 108-109 0.0016741024718122995 110-111 0.00243836664372661 112-113 0.004021485285549111 114-115 0.0025839407717102884 116-117 0.004858536521455261 118-119 0.0035119758376062375 120-121 0.007187722569194113 122-123 0.004185256179530749 124-125 0.055227184803807935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2747741.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.9382044515613 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39284969896622 54.98774773248758 2 9.263460494814764 12.216319040721144 3 3.1274339786660157 6.186521432821188 4 1.4073219037456774 3.7118511767337186 5 0.7828389591848213 2.58094976716881 6 0.4760850710521426 1.883531685082336 7 0.31359545579221754 1.4474544895375456 8 0.20688745311847048 1.091342974575081 9 0.14264432633127497 0.8465139678138157 >10 0.8162349186563577 9.600315224802912 >50 0.04764280344521115 2.141074719419235 >100 0.021572033871434845 2.4842244423434865 >500 0.00114632188436565 0.4928611653913418 >1k 2.8658047106601794E-4 0.329292181101797 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3143 0.11438487106317516 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.6393531995919555E-5 0.0 0.0 0.0 0.0 3 3.6393531995919555E-5 0.0 0.0 0.0 0.0 4 3.6393531995919555E-5 0.0 0.0 0.0 3.6393531995919555E-5 5 7.278706399183911E-5 0.0 0.0 0.0 3.6393531995919555E-5 6 7.278706399183911E-5 0.0 0.0 0.0 7.278706399183911E-5 7 7.278706399183911E-5 0.0 0.0 0.0 7.278706399183911E-5 8 7.278706399183911E-5 0.0 0.0 0.0 7.278706399183911E-5 9 7.278706399183911E-5 0.0 0.0 7.278706399183911E-5 7.278706399183911E-5 10-11 7.278706399183911E-5 0.0 0.0 9.098382998979889E-5 7.278706399183911E-5 12-13 7.278706399183911E-5 0.0 0.0 1.2737736198571844E-4 1.2737736198571844E-4 14-15 7.278706399183911E-5 0.0 0.0 1.4557412798367822E-4 1.63770893981638E-4 16-17 9.098382998979889E-5 0.0 0.0 1.63770893981638E-4 2.365579579734771E-4 18-19 1.2737736198571844E-4 0.0 0.0 2.1836119197551733E-4 2.9114825596735644E-4 20-21 1.4557412798367822E-4 0.0 0.0 3.6393531995919555E-4 3.27541787963276E-4 22-23 1.4557412798367822E-4 0.0 0.0 5.822965119347129E-4 3.821320859571553E-4 24-25 2.9114825596735644E-4 0.0 0.0 8.734447679020693E-4 4.003288519551151E-4 26-27 2.9114825596735644E-4 0.0 0.0 0.001146396257871466 5.095094479428738E-4 28-29 3.093450219653162E-4 0.0 0.0 0.0019470539617816962 5.459029799387933E-4 30-31 3.27541787963276E-4 0.0 0.0 0.0044946012014960655 5.459029799387933E-4 32-33 3.4573855396123577E-4 0.0 0.0 0.008607070317034976 7.278706399183911E-4 34-35 3.821320859571553E-4 0.0 0.0 0.01492134811832702 8.006577039102302E-4 36-37 4.003288519551151E-4 0.0 0.0 0.025366291801155932 8.006577039102302E-4 38-39 4.3672238395103466E-4 1.8196765997959777E-5 0.0 0.04580126001686476 8.006577039102302E-4 40-41 4.3672238395103466E-4 3.6393531995919555E-5 0.0 0.0708036164980615 8.370512359061498E-4 42-43 4.7311591594695427E-4 3.6393531995919555E-5 0.0 0.09520547970132556 8.370512359061498E-4 44-45 4.7311591594695427E-4 3.6393531995919555E-5 0.0 0.12326489287017955 8.370512359061498E-4 46-47 4.7311591594695427E-4 3.6393531995919555E-5 0.0 0.15925809601414398 9.098382998979889E-4 48-49 5.095094479428738E-4 3.6393531995919555E-5 0.0 0.19954573593362693 0.0010190188958857475 50-51 5.095094479428738E-4 3.6393531995919555E-5 0.0 0.24594748922842435 0.0010372156618837073 52-53 5.095094479428738E-4 3.6393531995919555E-5 0.0 0.29247661988520757 0.001146396257871466 54-55 5.095094479428738E-4 3.6393531995919555E-5 0.0 0.3417898557396785 0.0012373800878612649 56-57 5.459029799387933E-4 3.6393531995919555E-5 0.0 0.40411377928269077 0.0012555768538592246 58-59 5.459029799387933E-4 3.6393531995919555E-5 0.0 0.4722970614770461 0.0012737736198571844 60-61 5.640997459367531E-4 3.6393531995919555E-5 0.0 0.5522536512720813 0.0012737736198571844 62-63 5.822965119347129E-4 3.6393531995919555E-5 0.0 0.6353400848187657 0.001310167151853104 64-65 6.186900439306324E-4 1.0918059598775866E-4 0.0 0.7192635696013562 0.001310167151853104 66-67 6.55083575926552E-4 1.4557412798367822E-4 0.0 0.8150695425806145 0.001310167151853104 68-69 6.914771079224715E-4 1.4557412798367822E-4 0.0 0.9288539203658568 0.001310167151853104 70-71 6.914771079224715E-4 1.4557412798367822E-4 0.0 1.0634008081547714 0.0014011509818429029 72-73 6.914771079224715E-4 1.4557412798367822E-4 0.0 1.203879841659021 0.0015649218758245409 74-75 7.460674059163509E-4 1.4557412798367822E-4 0.0 1.3442133010352868 0.0016195121738184202 76-77 7.642641719143107E-4 1.4557412798367822E-4 0.0 1.5002323727017939 0.00163770893981638 78-79 7.642641719143107E-4 1.4557412798367822E-4 0.0 1.6853662699650367 0.00163770893981638 80-81 7.642641719143107E-4 1.4557412798367822E-4 0.0 1.8953023592834988 0.0016922992378102593 82-83 8.006577039102302E-4 1.4557412798367822E-4 0.0 2.120960454424198 0.0018560701317918975 84-85 8.916415339000291E-4 1.4557412798367822E-4 0.0 2.3437252637712214 0.0018560701317918975 86-87 9.82625363889828E-4 1.4557412798367822E-4 0.0 2.574187305135382 0.0018742668977898573 88-89 9.82625363889828E-4 1.4557412798367822E-4 0.0 2.836129751676013 0.0019106604297857769 90-91 0.0011281994918735062 1.4557412798367822E-4 0.0 3.1399975470759434 0.0019288571957837366 92-93 0.0011281994918735062 1.4557412798367822E-4 0.0 3.463863588307632 0.0020198410257735353 94-95 0.0011281994918735062 1.4557412798367822E-4 0.0 3.789021599925175 0.002038037791771495 96-97 0.0011281994918735062 1.4557412798367822E-4 0.0 4.114707317756659 0.002201808685753133 98-99 0.0011281994918735062 1.4557412798367822E-4 0.0 4.466559985093209 0.002292792515742932 100-101 0.0011281994918735062 1.4557412798367822E-4 0.0 4.870892125567876 0.002292792515742932 102-103 0.0011281994918735062 1.4557412798367822E-4 0.0 5.311253862718502 0.0023109892817408917 104-105 0.0013465606838490235 1.8196765997959777E-4 0.0 5.757711516478445 0.002365579579734771 106-107 0.0014557412798367822 1.8196765997959777E-4 0.0 6.193396684767596 0.0024019731117306906 108-109 0.0014557412798367822 1.8196765997959777E-4 0.0 6.655630934647771 0.00243836664372661 110-111 0.0014557412798367822 1.8196765997959777E-4 0.0 7.180043533942974 0.002529350473716409 112-113 0.0014557412798367822 1.8196765997959777E-4 0.0 7.739448514252253 0.0025839407717102884 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7230 0.0 50.47105 1 GGTATCA 4385 0.0 49.549973 1 ATCAACG 8490 0.0 40.435875 3 TATCAAC 9265 0.0 39.75062 2 CAACGCA 8840 0.0 39.554768 5 TCAACGC 8760 0.0 39.440716 4 AACGCAG 8950 0.0 38.25042 6 GTACATG 12385 0.0 36.528996 1 TACATGG 12555 0.0 34.357376 2 CGCAGAG 10475 0.0 31.888336 8 ACATGGG 12510 0.0 31.722486 3 ACGCAGA 10705 0.0 31.424541 7 GCAGAGT 11590 0.0 26.92023 9 CATGGGA 8160 0.0 26.207476 4 GAGTACT 5290 0.0 23.219896 12-13 CAGAGTA 11695 0.0 22.4419 10-11 AGAGTAC 10980 0.0 22.195906 10-11 CATGGGG 5275 0.0 21.682016 4 CGCATAG 310 8.385541E-9 21.128172 9 GAGTACA 9990 0.0 20.61725 1 >>END_MODULE