FastQCFastQC Report
Thu 26 May 2016
SRR1364474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2747741
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70960.2582485030430452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63290.23033466400217487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40240.1464475727515803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56900.059.2610661
GTATCAA101150.052.334851
ATCAACG125650.039.947673
TATCAAC132050.039.365872
TCAACGC129250.038.6505134
CAACGCA129450.038.468965
AACGCAG132500.036.73036
GTACATG128000.032.358081
TACATGG127000.031.3086222
CGCAGAG157100.030.926268
ACGCAGA157100.030.6757987
ACATGGG128400.029.1565693
GCAGAGT172400.027.1813189
GAGTACT101750.025.71672212-13
GTGGTAT30950.024.8749561
AGAGTAC159800.024.54343610-11
AGTACTT109950.024.01513912-13
CATGGGA80600.023.9635854
CAGAGTA165900.023.94569210-11
GTACTTT111800.023.77754414-15
Produced by FastQC (version 0.11.5)