##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364474_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2747741 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.99306776002542 31.0 28.0 31.0 25.0 34.0 2 29.2061824604284 31.0 28.0 34.0 25.0 34.0 3 29.294125610819943 31.0 28.0 34.0 25.0 34.0 4 32.72080811109926 35.0 32.0 37.0 26.0 37.0 5 31.836030033398345 35.0 32.0 37.0 22.0 37.0 6 31.879326690543248 35.0 32.0 37.0 22.0 37.0 7 31.31573463437784 35.0 31.0 37.0 17.0 37.0 8 31.441801101341067 35.0 32.0 37.0 17.0 37.0 9 32.0360576196956 37.0 30.0 39.0 11.0 39.0 10-11 32.230551751420535 37.0 31.0 39.0 16.5 39.0 12-13 31.968983430388818 37.0 30.5 39.0 13.5 39.0 14-15 32.92928900504087 38.0 31.0 40.0 14.0 41.0 16-17 32.99058062604882 38.0 31.0 40.0 15.5 41.0 18-19 32.860536528006094 38.0 31.0 40.0 11.0 41.0 20-21 32.74277379127072 38.0 31.0 40.0 10.5 41.0 22-23 32.71488506376693 38.0 31.0 40.0 10.5 41.0 24-25 32.516809080622956 38.0 31.0 40.0 10.0 41.0 26-27 32.59701533004748 37.5 31.0 40.0 10.0 41.0 28-29 32.4803665993265 37.0 30.5 40.0 9.5 41.0 30-31 31.953602068026065 37.0 30.0 40.0 8.0 41.0 32-33 31.87968644060703 37.0 30.0 40.0 8.0 41.0 34-35 31.673857725309627 37.0 30.0 40.0 7.0 41.0 36-37 31.64473871445671 37.0 30.0 40.0 2.0 41.0 38-39 31.410044469256746 37.0 30.0 40.0 2.0 41.0 40-41 31.11068110131195 36.0 29.5 40.0 2.0 41.0 42-43 30.98713070118326 36.0 29.0 40.0 2.0 41.0 44-45 30.904776687467997 36.0 29.0 40.0 2.0 41.0 46-47 30.7415453275982 36.0 28.5 40.0 2.0 41.0 48-49 30.45021983513002 36.0 28.0 40.0 2.0 41.0 50-51 30.086103821284468 36.0 27.0 40.0 2.0 41.0 52-53 29.985319940998806 35.0 27.0 40.0 2.0 41.0 54-55 29.51285656108054 35.0 26.0 39.5 2.0 41.0 56-57 29.527699481137414 35.0 26.0 39.0 2.0 41.0 58-59 29.282863814311465 35.0 26.0 39.0 2.0 41.0 60-61 29.002711682069016 35.0 26.0 39.0 2.0 41.0 62-63 28.730788673313825 34.0 26.0 39.0 2.0 40.5 64-65 28.251301887623324 34.0 24.0 38.0 2.0 40.0 66-67 27.868371509541838 34.0 23.0 38.0 2.0 40.0 68-69 27.67560261320117 34.0 22.5 37.5 2.0 40.0 70-71 27.755221834954604 34.0 24.5 37.0 2.0 40.0 72-73 27.532817139606678 34.0 24.0 37.0 2.0 39.0 74-75 27.169530352387653 34.0 22.5 36.0 2.0 39.0 76-77 27.087748808930684 33.5 24.0 36.0 2.0 39.0 78-79 26.691013818260164 33.0 22.0 35.0 2.0 37.5 80-81 26.46530986726915 33.0 22.0 35.0 2.0 37.0 82-83 26.32815392717145 33.0 22.0 35.0 2.0 37.0 84-85 25.895949436282386 33.0 20.0 35.0 2.0 36.0 86-87 25.766292565420102 33.0 20.0 35.0 2.0 36.0 88-89 25.726100276554448 33.0 20.0 35.0 2.0 36.0 90-91 25.564947897199918 33.0 20.0 35.0 2.0 35.0 92-93 25.588444107359464 33.0 20.0 35.0 2.0 35.0 94-95 25.59996611762171 33.0 20.0 35.0 2.0 35.0 96-97 25.483273168759354 33.0 20.0 35.0 2.0 35.0 98-99 25.347772770432147 33.0 19.5 35.0 2.0 35.0 100-101 25.250810211006062 33.0 18.5 35.0 2.0 35.0 102-103 24.12656323867497 30.5 17.5 34.0 2.0 35.0 104-105 25.076303043117967 32.0 19.0 35.0 2.0 35.0 106-107 25.173852084312166 33.0 18.0 35.0 2.0 35.0 108-109 25.245777167498684 33.0 19.0 35.0 2.0 35.0 110-111 25.174394165971247 33.0 18.0 35.0 2.0 35.0 112-113 25.126937546151545 33.0 18.0 35.0 2.0 35.0 114-115 25.074040457233778 33.0 17.5 35.0 2.0 35.0 116-117 24.994549522680632 33.0 15.5 35.0 2.0 35.0 118-119 24.87422923048424 33.0 8.5 35.0 2.0 35.0 120-121 24.713324691082605 33.0 2.0 35.0 2.0 35.0 122-123 24.43184910804912 33.0 2.0 35.0 2.0 35.0 124-125 23.65688541969567 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9049432197794864 1101 2 0.9180536765022254 1101 3 0.9045522490388755 1101 4 0.9073576371039067 1101 5 1.102137784257419 1101 6 1.1027976717481245 1101 7 1.6192228717731183 1101 8 1.4710396056202448 1101 9 2.1931947109229775 1101 10-11 1.8147640572458883 1101 12-13 1.958978329687742 1101 14-15 1.7764383268738158 1101 16-17 1.6989153202369707 1101 18-19 1.8812552370287392 1101 20-21 2.1163897133719303 1101 22-23 2.0970095323776903 1101 24-25 2.203926834810062 1101 26-27 1.9319162827450498 1101 28-29 1.9112140278734842 1101 30-31 2.437695840035449 1101 32-33 2.233316317149427 1101 34-35 2.307003369099707 1101 36-37 2.1777863716062598 1101 38-39 2.372128288467991 1101 40-41 2.5481563927637367 1101 42-43 2.5011816070639696 1101 44-45 2.3370423350016942 1101 46-47 2.1321353007043946 1101 48-49 2.175035340294251 1101 50-51 2.3667714266537345 1101 52-53 2.310437226353322 1101 54-55 2.625262049641769 1101 56-57 2.324835044399123 1101 58-59 2.5670294973564367 1101 60-61 2.5771718874131473 1101 62-63 2.5945367373657326 1101 64-65 2.653152579268852 1101 66-67 2.906018380836592 1101 68-69 2.784079720149734 1101 70-71 2.4226086200778525 1101 72-73 2.548156608250487 1101 74-75 2.696045020935859 1101 76-77 2.4863974643090074 1101 78-79 2.433456056631318 1101 80-81 2.469749583500736 1101 82-83 2.3480424259617934 1101 84-85 2.6868201180695834 1101 86-87 2.5353516545068295 1101 88-89 2.3307916682467216 1101 90-91 2.2449611602521706 1101 92-93 2.181970428119712 1101 94-95 2.2026660955002626 1101 96-97 2.116248970853455 1101 98-99 2.262186922270132 1101 100-101 2.3005746340435387 1101 102-103 2.1403025615389524 1101 104-105 2.0669549492579513 1101 106-107 2.1590388866586565 1101 108-109 2.1655848593771765 1101 110-111 2.1579228154005854 1101 112-113 2.0852727656412533 1101 114-115 2.0299964544202282 1101 116-117 2.0477514823377483 1101 118-119 2.001187450204416 1101 120-121 2.052695154716215 1101 122-123 2.1175611311631712 1101 124-125 2.0529429988861665 1102 1 0.24519374302197505 1102 2 -1.8890505385983687 1102 3 -0.1548248493931368 1102 4 0.22816603448818995 1102 5 0.7448792048195365 1102 6 0.2642484111541954 1102 7 1.1238851251000668 1102 8 0.8230751236860421 1102 9 1.5084739489641983 1102 10-11 1.2370523480286977 1102 12-13 1.357419738121873 1102 14-15 1.4399328220150451 1102 16-17 1.3296629060090552 1102 18-19 1.4412898998929222 1102 20-21 1.6230082180978371 1102 22-23 1.5316800768798302 1102 24-25 1.7409436213743845 1102 26-27 1.4950909578977445 1102 28-29 1.3942537925191445 1102 30-31 1.8084172124066349 1102 32-33 1.5321039417845803 1102 34-35 1.762538068526208 1102 36-37 1.1974094698081927 1102 38-39 1.1057430179112657 1102 40-41 1.3090603248184536 1102 42-43 1.1611861840423714 1102 44-45 1.122750848136942 1102 46-47 0.9925885129718601 1102 48-49 1.050425542751416 1102 50-51 1.2187585107907708 1102 52-53 1.0811887403455387 1102 54-55 1.360991652994091 1102 56-57 0.9539767862637802 1102 58-59 0.9313296677725269 1102 60-61 0.8797327324664899 1102 62-63 1.1658483125507964 1102 64-65 1.2369006943028324 1102 66-67 1.38083084288089 1102 68-69 1.282757178497672 1102 70-71 0.8135947148028038 1102 72-73 1.152724401482537 1102 74-75 1.2406330505210192 1102 76-77 1.1871650724108633 1102 78-79 1.0885858617898165 1102 80-81 1.1184838806525335 1102 82-83 0.7565063135353327 1102 84-85 0.9145788009520857 1102 86-87 0.8758655825218646 1102 88-89 1.0077731454180103 1102 90-91 0.727701515156383 1102 92-93 0.8614527457785108 1102 94-95 0.8609214775185556 1102 96-97 0.9368427875406766 1102 98-99 0.7109235937434057 1102 100-101 0.7178562276791958 1102 102-103 0.47738782442650063 1102 104-105 0.6610033278973511 1102 106-107 0.9515285957028397 1102 108-109 0.8244567289513469 1102 110-111 0.7192517621471204 1102 112-113 0.7790869559240576 1102 114-115 0.9746732591051988 1102 116-117 0.6401403997630872 1102 118-119 0.2540417636219878 1102 120-121 0.5616467925744466 1102 122-123 0.9473167075860047 1102 124-125 1.0460156094269948 1103 1 0.7712800812102003 1103 2 0.646575800645941 1103 3 0.5586027707333905 1103 4 0.6637520161362431 1103 5 0.9561226558621563 1103 6 1.0013176731315703 1103 7 1.2752765303298261 1103 8 1.237612921603425 1103 9 1.9012144294303646 1103 10-11 1.597336052611979 1103 12-13 1.6016908841489261 1103 14-15 1.3545514626984243 1103 16-17 1.3866733077967694 1103 18-19 1.5572603037306862 1103 20-21 1.785468759062681 1103 22-23 1.5847698679832334 1103 24-25 1.9501050730586442 1103 26-27 1.4467182520573658 1103 28-29 1.5974545115455214 1103 30-31 2.1862701161789566 1103 32-33 1.9066035209029941 1103 34-35 2.0899090883997182 1103 36-37 2.026570401214478 1103 38-39 2.0164122884083326 1103 40-41 2.2385419704321663 1103 42-43 2.289908972616928 1103 44-45 2.088339868252728 1103 46-47 2.0258944013647984 1103 48-49 1.9993337743698447 1103 50-51 2.1259322953925803 1103 52-53 1.9626528613553198 1103 54-55 2.2068906700348023 1103 56-57 1.994137392413137 1103 58-59 2.18037680473498 1103 60-61 2.040760678604297 1103 62-63 2.2209409242485947 1103 64-65 2.3900846734686425 1103 66-67 2.5037269896290084 1103 68-69 2.605608491484034 1103 70-71 2.378951944980436 1103 72-73 2.4483944906686084 1103 74-75 2.4490679711791827 1103 76-77 2.2006101534655116 1103 78-79 2.3241181960119484 1103 80-81 2.3823317611354753 1103 82-83 2.265438731978154 1103 84-85 2.4055984869837417 1103 86-87 2.3397415957327077 1103 88-89 2.2439406292206137 1103 90-91 2.3197365807824077 1103 92-93 2.0955172083302536 1103 94-95 2.012474152279818 1103 96-97 1.9609415307532139 1103 98-99 2.1395943434273583 1103 100-101 2.0485986266287917 1103 102-103 1.9351927539105311 1103 104-105 2.0079474491556226 1103 106-107 1.984872672942707 1103 108-109 1.9551138921695355 1103 110-111 1.9310969163291105 1103 112-113 1.8251032684689044 1103 114-115 1.79363518917895 1103 116-117 1.849234209764223 1103 118-119 1.9016046884139755 1103 120-121 1.8951441410426533 1103 122-123 2.0840152520945097 1103 124-125 1.9095378502564557 1104 1 0.7800405731620152 1104 2 0.6804421189283971 1104 3 0.6857289962970263 1104 4 0.6294147336214166 1104 5 1.0409283372499907 1104 6 1.2099936238758282 1104 7 1.604363037401697 1104 8 1.3012611170518404 1104 9 1.9043237185755935 1104 10-11 1.4538949825963883 1104 12-13 1.3580572071625774 1104 14-15 1.5420571795597127 1104 16-17 1.2710025683067059 1104 18-19 1.4306786387746158 1104 20-21 1.681461126277469 1104 22-23 1.7181721821476543 1104 24-25 2.0339360493103555 1104 26-27 1.7923715923129606 1104 28-29 1.7215930922583453 1104 30-31 2.237136250891023 1104 32-33 2.1498993116739733 1104 34-35 2.037976883908616 1104 36-37 1.9490707896981974 1104 38-39 2.1321483972502193 1104 40-41 2.1841026528514007 1104 42-43 2.0636966352990953 1104 44-45 1.777191767953905 1104 46-47 1.8833278928462285 1104 48-49 1.6403580971307505 1104 50-51 1.937253268319843 1104 52-53 1.8133474744581228 1104 54-55 2.095197867192553 1104 56-57 2.025897986979327 1104 58-59 1.9938288921279437 1104 60-61 2.1536184857813723 1104 62-63 2.2256516179491044 1104 64-65 2.411188696898364 1104 66-67 2.4222737545531423 1104 68-69 2.5564558906617307 1104 70-71 2.296383383122997 1104 72-73 2.4264908163380348 1104 74-75 2.4997757948313364 1104 76-77 2.3221431859368664 1104 78-79 2.3373592352852874 1104 80-81 2.272028930976351 1104 82-83 2.219590950099306 1104 84-85 2.4255212623072566 1104 86-87 2.3826764067971418 1104 88-89 2.257697132248893 1104 90-91 2.2036556466383352 1104 92-93 1.9653377057197936 1104 94-95 1.9748365191728858 1104 96-97 2.0071243864145316 1104 98-99 2.0486005736759303 1104 100-101 2.0482346137002203 1104 102-103 1.9399030270603248 1104 104-105 1.8774220172973486 1104 106-107 1.9395051074119678 1104 108-109 1.8870067026237578 1104 110-111 1.895984374854926 1104 112-113 1.8994771893620523 1104 114-115 1.7742703006637939 1104 116-117 1.8215418388275602 1104 118-119 1.8455622840750685 1104 120-121 1.7573742762770443 1104 122-123 1.9252693427387477 1104 124-125 1.788084643567764 1105 1 0.4427915315170665 1105 2 0.5018710931836381 1105 3 0.365173464269251 1105 4 0.5507309270498411 1105 5 0.7928171687027223 1105 6 0.9012751307357867 1105 7 1.1623931833687564 1105 8 0.91806067192989 1105 9 1.5107066622073155 1105 10-11 1.1487312296028946 1105 12-13 1.3262696188207848 1105 14-15 1.324539978328879 1105 16-17 1.2564401789833326 1105 18-19 1.2996064409562536 1105 20-21 1.4895200287483945 1105 22-23 1.4490271074211805 1105 24-25 1.6053130703259626 1105 26-27 1.3485561346892467 1105 28-29 1.4693365712622182 1105 30-31 1.6694161473867588 1105 32-33 1.5841225732329143 1105 34-35 1.6953825641720393 1105 36-37 1.419039955064747 1105 38-39 1.4678976049965726 1105 40-41 1.618008209576253 1105 42-43 1.5816161136832392 1105 44-45 1.4915075536305373 1105 46-47 1.326350990410635 1105 48-49 1.3465653733721652 1105 50-51 1.5043420588271417 1105 52-53 1.4363037941534067 1105 54-55 1.6712846502245462 1105 56-57 1.4652138631019476 1105 58-59 1.4808850337616768 1105 60-61 1.2990334221808162 1105 62-63 1.1569061761608879 1105 64-65 1.6024555195861296 1105 66-67 1.845775734894655 1105 68-69 1.9468948346186288 1105 70-71 1.744966275381394 1105 72-73 1.847677379384102 1105 74-75 1.9205550849133246 1105 76-77 1.7330574256893883 1105 78-79 1.8886753095378275 1105 80-81 1.8526498777990064 1105 82-83 1.725115790079073 1105 84-85 1.9960310127039946 1105 86-87 1.9347910127175894 1105 88-89 1.7582087639221875 1105 90-91 1.7996304383837867 1105 92-93 1.6579213953925809 1105 94-95 1.5480224599505554 1105 96-97 1.593716869991134 1105 98-99 1.6628836019438147 1105 100-101 1.695991462749273 1105 102-103 1.4154391961981503 1105 104-105 1.1106117259484733 1105 106-107 1.411976035411218 1105 108-109 1.4096032494597743 1105 110-111 1.5098997401811651 1105 112-113 1.5148165203639365 1105 114-115 1.4507485126508826 1105 116-117 1.4375868613507379 1105 118-119 1.5694983838780665 1105 120-121 1.4860120245592618 1105 122-123 1.5987768828483517 1105 124-125 1.5107049404561899 1106 1 0.4002226212057174 1106 2 0.2811889037533213 1106 3 0.23338340605414842 1106 4 0.16914267927682403 1106 5 0.3657973217119981 1106 6 0.5941493441624921 1106 7 0.7393020393552518 1106 8 0.38588573730401876 1106 9 1.01920073576375 1106 10-11 0.6032297651130492 1106 12-13 0.6028875428159246 1106 14-15 0.7582465276132311 1106 16-17 0.6609290443815965 1106 18-19 0.7123557916018797 1106 20-21 0.579475303600816 1106 22-23 0.453019257814141 1106 24-25 0.3248723515238794 1106 26-27 0.45476375077296893 1106 28-29 0.5552881869074682 1106 30-31 0.8689131553387845 1106 32-33 0.6865436269647738 1106 34-35 0.7498817550076247 1106 36-37 0.598112175663104 1106 38-39 0.6431344117116993 1106 40-41 0.8880996610511112 1106 42-43 0.8259820346166613 1106 44-45 0.8760339339719856 1106 46-47 0.7447438302709308 1106 48-49 0.7421156541899379 1106 50-51 1.084852740387781 1106 52-53 0.667408961481005 1106 54-55 1.279168355850416 1106 56-57 0.917746774384046 1106 58-59 0.9037659591288332 1106 60-61 1.1616691984319196 1106 62-63 1.134393798228107 1106 64-65 1.250660694284786 1106 66-67 1.4238463312066685 1106 68-69 1.31249631043314 1106 70-71 1.1113383543157198 1106 72-73 1.0187378405198118 1106 74-75 1.3043372105496047 1106 76-77 0.905944836611841 1106 78-79 0.909238771332813 1106 80-81 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98-99 -4.200106510769153 2216 100-101 -4.487323274835479 2216 102-103 -4.4784692064813925 2216 104-105 -4.262099010063679 2216 106-107 -4.416258036068928 2216 108-109 -4.467764520858147 2216 110-111 -4.111113653948731 2216 112-113 -4.154349211979547 2216 114-115 -4.117004125910206 2216 116-117 -4.020732262055468 2216 118-119 -4.206062992448896 2216 120-121 -4.174704657724671 2216 122-123 -4.136507986901698 2216 124-125 -3.998228805240956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 134096.0 3 45354.0 4 21833.0 5 18026.0 6 18609.0 7 21537.0 8 24290.0 9 25249.0 10 27293.0 11 29029.0 12 31529.0 13 33862.0 14 35189.0 15 28671.0 16 20797.0 17 18024.0 18 16060.0 19 14736.0 20 14432.0 21 15609.0 22 19130.0 23 25829.0 24 35366.0 25 46766.0 26 57650.0 27 65117.0 28 73620.0 29 84127.0 30 93468.0 31 105807.0 32 123575.0 33 147226.0 34 178628.0 35 226945.0 36 317759.0 37 405449.0 38 146417.0 39 637.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.01341313648007 19.743227582870325 14.255492234567042 23.987867046082563 2 15.767325282332745 20.959387247596474 38.66620228384636 24.607085186224424 3 19.12618401807157 25.34412814017042 29.56020964130171 25.969478200456305 4 12.69095349228092 17.191219466309164 36.51704913573936 33.600777905670554 5 13.046826465813192 38.85133278573199 33.84722213629305 14.254618612161774 6 31.690710071080257 37.459234665301175 16.91084924732662 13.93920601629194 7 27.735810382534154 32.92165278520586 20.69414706836436 18.64838976389563 8 26.31805544991322 35.76981964457349 19.175060531542094 18.7370643739712 9 25.925951536189185 16.02585542087118 19.81922604786987 38.22896699506977 10-11 24.93924057819532 27.086764963850058 26.980440524914822 20.993553933039806 12-13 25.681141381635154 24.31127961429875 27.733240893139683 22.27433811092641 14-15 22.525195820910188 25.621791098280305 25.839807622844695 26.013205457964812 16-17 22.049620946051686 28.96558124638325 26.457292284623474 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22.3141125992346 26.397627704584732 28.67955723994288 22.608702456237783 44-45 22.17353328129009 26.65392880993844 28.664945236314537 22.507592672456934 46-47 22.454530731675362 26.891421479873422 28.03279792265266 22.621249865798557 48-49 22.505222473742055 26.935645293950667 27.782577682022318 22.77655455028496 50-51 22.568485307893983 27.027707964469666 27.53171089753882 22.87209583009753 52-53 22.773810981717883 27.04273822172735 27.501096810669278 22.682353985885488 54-55 22.47317231743218 26.979596165297735 27.48996535706711 23.057266160202975 56-57 22.457810243311354 27.30984380041085 27.339850338524336 22.89249561775346 58-59 22.53791387179505 26.89252735246881 27.530360394229298 23.039198381506846 60-61 22.405677973287872 27.001271226072614 27.78078428789322 22.812266512746287 62-63 22.41765084591922 26.971097138319355 27.83768803310089 22.773563982660537 64-65 22.278276667805883 26.8233298088648 27.669875954123764 23.228517569205554 66-67 22.174354500341636 27.175480543843513 27.44338240528354 23.206782550531315 68-69 22.406427359112186 26.909207238148824 27.712445156233876 22.97192024650511 70-71 22.314518956952273 26.87707483405422 27.696535906286783 23.111870302706723 72-73 22.379861116801553 26.9400445948542 27.422594058450393 23.25750022989385 74-75 22.240391525227256 27.271848824443467 27.25756370028026 23.230195950049016 76-77 22.3791216659955 27.097265867462145 27.434276167566907 23.08933629897545 78-79 22.448751200322743 27.092182784974405 27.37758527727842 23.08148073742443 80-81 22.302775393492063 27.289024984746536 27.316975263093823 23.09122435866758 82-83 22.39471565242313 27.211205582774916 27.287268161532896 23.106810603269054 84-85 22.233099844563224 27.13378007606976 27.15918276140291 23.473937317964104 86-87 22.374721702349117 26.98459548009131 27.34960553838483 23.291077279174736 88-89 22.32446325127807 26.998206864575145 27.30291614698979 23.374413737156992 90-91 22.191878037729257 27.265820757824173 27.327944652595683 23.214356551850887 92-93 22.263812887861516 27.109121391021546 27.352435883601373 23.274629837515562 94-95 22.13973678339423 26.898977770967853 27.354808137797654 23.60647730784027 96-97 22.28866617971022 26.801948116937968 27.39008086461337 23.519304838738442 98-99 21.94096896323198 26.809277148028144 27.292565056167955 23.957188832571923 100-101 21.90060259325274 26.833132711542255 27.511716585553593 23.75454810965141 102-103 22.025297890107705 26.516909873150247 27.555979101790424 23.90181313495162 104-105 21.88258067967654 26.648526867689316 27.546084800436432 23.92280765219771 106-107 21.750624467858035 26.70549737338638 27.418847383458296 24.125030775297294 108-109 21.981911686727386 26.67884272935477 27.141786653108863 24.19745893080898 110-111 21.67736335563655 26.73074368525349 27.42386001561286 24.168032943497096 112-113 21.905794096340752 26.78597456488277 27.358664122269367 23.949567216507113 114-115 21.622852118461868 26.89708420003155 27.280909213820177 24.199154467686405 116-117 21.561766556600496 26.82323406754858 27.418686841299817 24.196312534551108 118-119 21.426139534663665 26.937798964702083 27.205312493687927 24.430749006946325 120-121 21.33979648637932 26.953189756370694 27.389285172919614 24.31772858433037 122-123 21.412643937519107 26.929981948670168 27.254833097986698 24.402541015824028 124-125 21.32563089859454 26.882386522146533 27.192610670786244 24.599371908472683 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 235.0 1 200.0 2 317.0 3 516.0 4 413.0 5 309.0 6 591.0 7 1374.0 8 2312.0 9 2712.0 10 2281.0 11 1677.5 12 1542.5 13 1642.0 14 1792.5 15 2066.0 16 2357.0 17 2646.5 18 3090.0 19 3500.5 20 3948.5 21 4702.0 22 5656.5 23 7041.5 24 8810.5 25 10953.0 26 14272.5 27 18451.5 28 22734.0 29 27361.5 30 33008.0 31 40009.5 32 46991.5 33 52173.5 34 56674.0 35 61438.0 36 67224.5 37 73607.5 38 77784.5 39 80079.0 40 82425.0 41 85668.0 42 88177.0 43 90214.0 44 93408.5 45 96267.5 46 97238.5 47 97735.5 48 98890.0 49 97890.0 50 95481.0 51 93826.5 52 92546.0 53 92333.5 54 91998.0 55 86765.0 56 78861.5 57 71864.0 58 66583.0 59 60728.5 60 52064.0 61 43732.0 62 36120.0 63 27706.5 64 20464.0 65 15663.5 66 11947.0 67 9510.0 68 7216.5 69 5230.5 70 3894.0 71 2786.0 72 2126.5 73 1756.5 74 1359.5 75 1065.0 76 749.5 77 415.5 78 241.5 79 149.0 80 98.5 81 57.5 82 34.5 83 29.0 84 15.0 85 7.5 86 7.5 87 5.5 88 2.5 89 0.5 90 1.5 91 1.0 92 1.0 93 1.0 94 0.0 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10262976022849316 2 2.911482559673565E-4 3 0.0 4 0.18167651172363045 5 0.0 6 7.278706399183912E-5 7 0.03755812501978899 8 0.0 9 0.0 10-11 6.914771079224717E-4 12-13 6.732803419245118E-4 14-15 4.913126819449141E-4 16-17 0.004039682051547071 18-19 0.00165590570581434 20-21 0.0030024663896633633 22-23 0.0017832830678000582 24-25 0.004640175329479744 26-27 0.004367223839510347 28-29 0.003111646985651122 30-31 8.916415339000291E-4 32-33 3.639353199591956E-5 34-35 0.0012555768538592246 36-37 0.0015285283438286215 38-39 5.82296511934713E-4 40-41 0.001710496003808219 42-43 8.188544699081901E-4 44-45 9.462318318939085E-4 46-47 2.1836119197551733E-4 48-49 3.639353199591956E-5 50-51 0.0411246911553891 52-53 5.459029799387933E-5 54-55 0.1363665643887106 56-57 2.3655795797347713E-4 58-59 0.0 60-61 0.0 62-63 7.278706399183911E-4 64-65 0.061887201159061205 66-67 0.050932748028289426 68-69 0.001801479833798018 70-71 0.0029660728576674437 72-73 0.06945705581421248 74-75 0.004640175329479744 76-77 0.0018378733657939375 78-79 0.0015467251098265813 80-81 1.63770893981638E-4 82-83 1.2737736198571844E-4 84-85 0.0 86-87 3.093450219653162E-4 88-89 0.0014557412798367822 90-91 2.1836119197551733E-4 92-93 0.002274595749744972 94-95 0.0042944367755185075 96-97 0.0022563989837470124 98-99 0.0 100-101 0.001219183321863305 102-103 0.0015103315778306615 104-105 2.7295148996939666E-4 106-107 5.459029799387933E-5 108-109 0.0 110-111 2.1836119197551733E-4 112-113 1.63770893981638E-4 114-115 1.63770893981638E-4 116-117 0.0 118-119 0.0010736091938796269 120-121 0.002274595749744972 122-123 7.642641719143108E-4 124-125 0.007697232017136986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2747741.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.1442395740102 #Duplication Level Percentage of deduplicated Percentage of total 1 79.37522869338176 46.15212313389838 2 11.083922094933769 12.889324434149879 3 3.9432206173122104 6.878266927985327 4 1.8236666490308 4.2414284217751685 5 1.0012519626408474 2.910851699486867 6 0.6028620538307048 2.1031773412807397 7 0.4141884829909622 1.6857872068675648 8 0.29361957552842394 1.365782955451509 9 0.20126144458996134 1.053197428612508 >10 1.1419187116960523 12.03385259210589 >50 0.07945500402221524 3.1680652982218076 >100 0.03679003737999327 3.7616241044426935 >500 0.0019131751188650094 0.7560619643367432 >1k 5.739525356435154E-4 0.5026569881476719 >5k 1.2754500792078118E-4 0.49779950323712713 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7096 0.2582485030430452 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6329 0.23033466400217487 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4024 0.1464475727515803 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.278706399183911E-5 2 0.0 0.0 0.0 0.0 7.278706399183911E-5 3 0.0 0.0 0.0 0.0 7.278706399183911E-5 4 0.0 0.0 0.0 0.0 7.278706399183911E-5 5 0.0 0.0 0.0 0.0 7.278706399183911E-5 6 0.0 0.0 0.0 0.0 1.0918059598775866E-4 7 0.0 0.0 0.0 0.0 1.0918059598775866E-4 8 0.0 0.0 0.0 0.0 1.4557412798367822E-4 9 0.0 0.0 0.0 0.0 1.4557412798367822E-4 10-11 0.0 0.0 0.0 0.0 1.63770893981638E-4 12-13 7.278706399183911E-5 0.0 0.0 0.0 3.4573855396123577E-4 14-15 7.278706399183911E-5 0.0 0.0 0.0 5.095094479428738E-4 16-17 7.278706399183911E-5 0.0 0.0 1.8196765997959777E-5 5.459029799387933E-4 18-19 9.098382998979889E-5 0.0 0.0 3.6393531995919555E-5 5.822965119347129E-4 20-21 1.0918059598775866E-4 0.0 0.0 1.4557412798367822E-4 6.914771079224715E-4 22-23 1.0918059598775866E-4 0.0 0.0 4.003288519551151E-4 8.370512359061498E-4 24-25 1.4557412798367822E-4 0.0 0.0 6.732803419245118E-4 8.734447679020693E-4 26-27 1.4557412798367822E-4 0.0 0.0 9.280350658959487E-4 8.734447679020693E-4 28-29 1.63770893981638E-4 0.0 0.0 0.0017832830678000582 8.916415339000291E-4 30-31 1.8196765997959777E-4 0.0 0.0 0.004221649711526668 9.462318318939084E-4 32-33 1.8196765997959777E-4 0.0 0.0 0.008133954401088021 9.82625363889828E-4 34-35 2.0016442597755755E-4 0.0 0.0 0.014411838670384144 0.0010190188958857475 36-37 2.365579579734771E-4 0.0 0.0 0.025147930609180413 0.0010190188958857475 38-39 2.547547239714369E-4 0.0 0.0 0.045237160270928015 0.0010190188958857475 40-41 2.547547239714369E-4 0.0 0.0 0.06976640083617779 0.0010190188958857475 42-43 2.547547239714369E-4 1.8196765997959777E-5 0.0 0.09485974114736433 0.0010736091938796269 44-45 2.547547239714369E-4 3.6393531995919555E-5 0.0 0.12366522172213465 0.0011281994918735062 46-47 3.6393531995919555E-4 3.6393531995919555E-5 0.0 0.16064105022998892 0.0011827897898673855 48-49 4.003288519551151E-4 5.459029799387933E-5 0.0 0.20121983840543922 0.001219183321863305 50-51 4.003288519551151E-4 7.278706399183911E-5 0.0 0.2481129043821816 0.0012737736198571844 52-53 4.003288519551151E-4 7.278706399183911E-5 0.0 0.2946238382729668 0.0013465606838490235 54-55 4.003288519551151E-4 7.278706399183911E-5 0.0 0.34340936791349697 0.0014193477478408626 56-57 4.003288519551151E-4 7.278706399183911E-5 0.0 0.40573329145650916 0.0014193477478408626 58-59 4.003288519551151E-4 7.278706399183911E-5 0.0 0.4728429644569848 0.0014193477478408626 60-61 4.003288519551151E-4 7.278706399183911E-5 0.0 0.5516349612281507 0.0014193477478408626 62-63 4.003288519551151E-4 9.098382998979889E-5 0.0 0.630699909489286 0.00163770893981638 64-65 4.3672238395103466E-4 1.0918059598775866E-4 0.0 0.7112569925622538 0.0016559057058143398 66-67 5.459029799387933E-4 1.0918059598775866E-4 0.0 0.8037693508958814 0.0017286927698061789 68-69 5.822965119347129E-4 1.0918059598775866E-4 0.0 0.9138597851835379 0.0017832830678000584 70-71 5.822965119347129E-4 1.0918059598775866E-4 0.0 1.0453678130507935 0.0018014798337980182 72-73 5.822965119347129E-4 1.0918059598775866E-4 0.0 1.181643393609514 0.002038037791771495 74-75 5.822965119347129E-4 1.0918059598775866E-4 0.0 1.315607984886494 0.0020744313237674146 76-77 5.822965119347129E-4 1.0918059598775866E-4 0.0 1.4637114633438886 0.0020744313237674146 78-79 5.822965119347129E-4 1.0918059598775866E-4 0.0 1.6392556649262064 0.0020926280897653744 80-81 6.368868099285922E-4 1.0918059598775866E-4 0.0 1.8384738590718703 0.0021472183877592537 82-83 6.914771079224715E-4 1.0918059598775866E-4 0.0 2.0550153744475916 0.002292792515742932 84-85 6.914771079224715E-4 1.2737736198571844E-4 0.0 2.2690093425835984 0.002292792515742932 86-87 6.914771079224715E-4 1.4557412798367822E-4 0.0 2.4909552974607143 0.0024201698777286504 88-89 6.914771079224715E-4 1.4557412798367822E-4 0.0 2.7450549378562243 0.0025839407717102884 90-91 8.006577039102302E-4 1.4557412798367822E-4 0.0 3.042444684560881 0.0025839407717102884 92-93 8.370512359061498E-4 1.4557412798367822E-4 0.0 3.3598144803312975 0.0027477116656919264 94-95 8.370512359061498E-4 1.4557412798367822E-4 0.0 3.6807508422373143 0.0028023019636858057 96-97 8.370512359061498E-4 1.4557412798367822E-4 0.0 4.001487039717353 0.003038859921659283 98-99 8.916415339000291E-4 1.63770893981638E-4 0.0 4.354031184161826 0.0032390243476368404 100-101 9.280350658959488E-4 1.8196765997959777E-4 0.0 4.764677602437784 0.0032390243476368404 102-103 9.462318318939085E-4 1.8196765997959777E-4 0.0 5.21397395169341 0.00327541787963276 104-105 0.0011281994918735062 1.8196765997959777E-4 0.0 5.665999815848728 0.00327541787963276 106-107 0.0012919703858551442 1.8196765997959777E-4 0.0 6.108781722877083 0.0033118114116286795 108-109 0.001310167151853104 2.0016442597755755E-4 0.0 6.580168946054231 0.0033845984756205186 110-111 0.001310167151853104 2.1836119197551733E-4 0.0 7.113279599496459 0.003475582305610318 112-113 0.001310167151853104 2.1836119197551733E-4 0.0 7.681309846888771 0.003566566135600117 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5690 0.0 59.261066 1 GTATCAA 10115 0.0 52.33485 1 ATCAACG 12565 0.0 39.94767 3 TATCAAC 13205 0.0 39.36587 2 TCAACGC 12925 0.0 38.650513 4 CAACGCA 12945 0.0 38.46896 5 AACGCAG 13250 0.0 36.7303 6 GTACATG 12800 0.0 32.35808 1 TACATGG 12700 0.0 31.308622 2 CGCAGAG 15710 0.0 30.92626 8 ACGCAGA 15710 0.0 30.675798 7 ACATGGG 12840 0.0 29.156569 3 GCAGAGT 17240 0.0 27.181318 9 GAGTACT 10175 0.0 25.716722 12-13 GTGGTAT 3095 0.0 24.874956 1 AGAGTAC 15980 0.0 24.543436 10-11 AGTACTT 10995 0.0 24.015139 12-13 CATGGGA 8060 0.0 23.963585 4 CAGAGTA 16590 0.0 23.945692 10-11 GTACTTT 11180 0.0 23.777544 14-15 >>END_MODULE