FastQCFastQC Report
Thu 26 May 2016
SRR1364473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546420
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44000.054.250511
GTATCAA79800.053.409961
TCAACGC92850.043.6846354
ATCAACG92800.043.599663
CAACGCA96450.042.485185
AACGCAG96050.042.1422356
TATCAAC105550.040.1927152
ACGCAGA113850.034.8234447
CGCAGAG111300.034.6614848
GTACATG125050.034.4641041
TACATGG129950.032.1422922
ACATGGG124900.030.7269483
GCAGAGT126000.028.1606739
CAGAGTA122200.024.3010510-11
AGAGTAC116300.023.76849610-11
GAGTACT64200.022.51653312-13
CATGGGG63900.022.3704094
CATGGGA79250.022.1710514
GAGTACA96050.021.4432621
TATAACG2306.145261E-620.6956082
Produced by FastQC (version 0.11.5)