FastQCFastQC Report
Thu 26 May 2016
SRR1364473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3546420
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72800.20527743470880494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65210.18387557029342266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40930.1154121621240575No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58450.067.174911
GTATCAA104700.052.1482931
ATCAACG132250.039.4777373
TCAACGC132750.039.1045654
TATCAAC139250.038.7344322
CAACGCA134900.038.3677525
AACGCAG136350.037.2179956
ACGCAGA162000.031.1048017
CGCAGAG163750.031.0875248
GTACATG122000.029.1509151
TACATGG124600.027.3125292
GCAGAGT185200.026.4593949
ACATGGG119500.026.4333253
GAGTACT111500.025.84374612-13
CAGAGTA174800.024.65094410-11
GTGGTAT29200.024.3181111
AGAGTAC167450.023.9925710-11
GTACTTT123650.023.59290914-15
AGTACTT121000.022.9053612-13
TGGTATC30950.021.1824342
Produced by FastQC (version 0.11.5)