##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364473_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3546420 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.924406302693985 31.0 28.0 31.0 25.0 34.0 2 29.155444645586254 31.0 28.0 34.0 25.0 34.0 3 29.20230824324248 31.0 28.0 34.0 25.0 34.0 4 32.64435374264752 35.0 32.0 37.0 26.0 37.0 5 31.742476920387322 35.0 32.0 37.0 20.0 37.0 6 31.78123516109203 35.0 32.0 37.0 21.0 37.0 7 31.249592264875563 35.0 31.0 37.0 17.0 37.0 8 31.358575126465563 35.0 32.0 37.0 17.0 37.0 9 31.95937649798952 37.0 30.0 39.0 11.0 39.0 10-11 32.140668899904696 37.0 31.0 39.0 15.5 39.0 12-13 31.885598575464833 37.0 30.5 39.0 12.5 39.0 14-15 32.83569134507475 38.0 31.0 40.0 12.0 41.0 16-17 32.89545146936911 38.0 31.0 40.0 12.0 41.0 18-19 32.75600154522025 38.0 31.0 40.0 10.5 41.0 20-21 32.63933346868109 38.0 31.0 40.0 10.5 41.0 22-23 32.60593302541718 38.0 31.0 40.0 10.0 41.0 24-25 32.41351884435572 38.0 30.0 40.0 10.0 41.0 26-27 32.49409742782863 37.0 31.0 40.0 10.0 41.0 28-29 32.37969205565049 37.0 30.0 40.0 9.5 41.0 30-31 31.87086766372849 37.0 30.0 40.0 8.0 41.0 32-33 31.817793154787083 37.0 30.0 40.0 8.0 41.0 34-35 31.627936764399028 37.0 30.0 40.0 4.5 41.0 36-37 31.606606380518947 37.0 30.0 40.0 2.0 41.0 38-39 31.38054263172439 37.0 30.0 40.0 2.0 41.0 40-41 31.10433733173172 36.0 29.5 40.0 2.0 41.0 42-43 30.98733032184569 36.0 29.0 40.0 2.0 41.0 44-45 30.915584166567974 36.0 29.0 40.0 2.0 41.0 46-47 30.770906435222 36.0 28.5 40.0 2.0 41.0 48-49 30.508280322127668 36.0 28.0 40.0 2.0 41.0 50-51 30.17359633094783 36.0 27.5 40.0 2.0 41.0 52-53 30.07908778430079 36.0 27.5 40.0 2.0 41.0 54-55 29.61179598017155 35.0 26.0 40.0 2.0 41.0 56-57 29.627075332306944 35.0 27.0 39.0 2.0 41.0 58-59 29.389230125027495 35.0 26.0 39.0 2.0 41.0 60-61 29.107208819034405 35.0 26.0 39.0 2.0 41.0 62-63 28.84283474602557 34.5 26.0 39.0 2.0 41.0 64-65 28.376549026906005 34.0 25.0 38.5 2.0 40.0 66-67 27.989021322911555 34.0 23.0 38.0 2.0 40.0 68-69 27.804838823376812 34.0 23.0 37.5 2.0 40.0 70-71 27.873162654169555 34.0 24.5 37.0 2.0 40.0 72-73 27.64588909379036 34.0 24.5 37.0 2.0 39.0 74-75 27.273552765887853 34.0 23.5 36.0 2.0 39.0 76-77 27.18289204324361 34.0 24.0 36.0 2.0 39.0 78-79 26.792673033650836 33.0 23.0 35.5 2.0 37.5 80-81 26.56146451914889 33.0 22.5 35.0 2.0 37.0 82-83 26.41871380152379 33.0 23.0 35.0 2.0 37.0 84-85 25.993994648124023 33.0 20.5 35.0 2.0 36.0 86-87 25.85588537172698 33.0 20.0 35.0 2.0 36.0 88-89 25.81025795027098 33.0 20.0 35.0 2.0 36.0 90-91 25.652025704795257 33.0 20.0 35.0 2.0 35.0 92-93 25.66799321569357 33.0 20.0 35.0 2.0 35.0 94-95 25.674416876737666 33.0 20.0 35.0 2.0 35.0 96-97 25.55822420920252 33.0 20.0 35.0 2.0 35.0 98-99 25.420245768972656 33.0 19.5 35.0 2.0 35.0 100-101 25.32866919315817 33.0 19.0 35.0 2.0 35.0 102-103 24.208656053146555 30.5 17.5 34.0 2.0 35.0 104-105 25.148934136396704 32.5 19.0 35.0 2.0 35.0 106-107 25.251099700543083 33.0 19.0 35.0 2.0 35.0 108-109 25.321414271293303 33.0 19.0 35.0 2.0 35.0 110-111 25.256074435628044 33.0 18.5 35.0 2.0 35.0 112-113 25.210803147963297 33.0 18.0 35.0 2.0 35.0 114-115 25.162156625554786 33.0 18.0 35.0 2.0 35.0 116-117 25.082951116900986 33.0 17.0 35.0 2.0 35.0 118-119 24.958905318602987 33.0 11.0 35.0 2.0 35.0 120-121 24.797458422860238 33.0 2.0 35.0 2.0 35.0 122-123 24.513638401542963 33.0 2.0 35.0 2.0 35.0 124-125 23.747658624753974 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8277531154739037 1101 2 0.8378754606969423 1101 3 0.851550537956701 1101 4 0.8320778120451635 1101 5 1.009342303010623 1101 6 1.0414912186159135 1101 7 1.365660576544542 1101 8 1.2851482960483125 1101 9 1.8867798734479138 1101 10-11 1.6385397178934156 1101 12-13 1.842752112766405 1101 14-15 1.8125279515131965 1101 16-17 1.7176723613102283 1101 18-19 1.7532672329039443 1101 20-21 1.980778832710584 1101 22-23 1.9283000543974964 1101 24-25 2.066853813413182 1101 26-27 1.8084164305145052 1101 28-29 1.8780947106855805 1101 30-31 2.3453290123880777 1101 32-33 2.0518256135846684 1101 34-35 2.158804314091295 1101 36-37 1.944389334453966 1101 38-39 2.158762171530338 1101 40-41 2.2612286245963986 1101 42-43 2.2492707422776945 1101 44-45 2.0606708900576614 1101 46-47 1.8786702658763232 1101 48-49 1.933611220894214 1101 50-51 2.1390934167630853 1101 52-53 2.056757654949351 1101 54-55 2.365387197353865 1101 56-57 1.9782232776443252 1101 58-59 2.253978009391542 1101 60-61 2.340504988620804 1101 62-63 2.300125354166159 1101 64-65 2.481771518634879 1101 66-67 2.5498912398761533 1101 68-69 2.5504434570296475 1101 70-71 2.123081779762945 1101 72-73 2.3346871025013662 1101 74-75 2.4434150165135726 1101 76-77 2.254422560536277 1101 78-79 2.294224190900181 1101 80-81 2.284492020016234 1101 82-83 2.107417362181856 1101 84-85 2.402856699367277 1101 86-87 2.2430197767704527 1101 88-89 2.108814930883028 1101 90-91 2.0340129224873493 1101 92-93 1.9562330075953192 1101 94-95 1.9070805260764985 1101 96-97 1.8234094309284856 1101 98-99 1.9350253491086207 1101 100-101 2.012970754242719 1101 102-103 1.8248296748807604 1101 104-105 1.831205955340721 1101 106-107 1.9205702881159645 1101 108-109 1.958823178775333 1101 110-111 1.944218067981211 1101 112-113 1.8948609492131254 1101 114-115 1.8866816152169577 1101 116-117 1.9129051671623962 1101 118-119 1.7913515966875977 1101 120-121 1.7820811027532741 1101 122-123 1.9319704008730838 1101 124-125 1.7225203301607053 1102 1 0.5119480585810265 1102 2 -1.622142559158899 1102 3 0.009307292569328496 1102 4 0.4073792763001123 1102 5 0.860123163430405 1102 6 0.5923148620267646 1102 7 1.2193359471415803 1102 8 0.9869471649906956 1102 9 1.7950327407583089 1102 10-11 1.4889114482528996 1102 12-13 1.647470425019911 1102 14-15 1.5643551535622393 1102 16-17 1.5350115142636653 1102 18-19 1.7323627834728725 1102 20-21 1.7547057162955113 1102 22-23 1.7409863445738338 1102 24-25 1.9569546509398137 1102 26-27 1.7096586955763868 1102 28-29 1.6843322441186572 1102 30-31 2.043576609740633 1102 32-33 1.660443269643423 1102 34-35 1.9527124128264965 1102 36-37 1.4372100085552333 1102 38-39 1.3085207734615274 1102 40-41 1.608058574956793 1102 42-43 1.3546164911783691 1102 44-45 1.325904043192434 1102 46-47 1.0259840040330843 1102 48-49 1.0343171402468556 1102 50-51 1.3191313264598072 1102 52-53 1.0716644106953055 1102 54-55 1.4123198552259346 1102 56-57 0.9766822399726287 1102 58-59 1.0336910800203114 1102 60-61 1.0429142943463567 1102 62-63 1.321503958137324 1102 64-65 1.4819715503679554 1102 66-67 1.5285959352385916 1102 68-69 1.5708075941165554 1102 70-71 0.920316043555502 1102 72-73 1.2408811446156989 1102 74-75 1.4748576816389196 1102 76-77 1.3559393734017746 1102 78-79 1.2115045526572885 1102 80-81 1.2238230470346885 1102 82-83 0.946340534129817 1102 84-85 0.9710938701365777 1102 86-87 1.1707727630798956 1102 88-89 1.2324353919193385 1102 90-91 1.031455434613921 1102 92-93 1.05677176161862 1102 94-95 1.1164704009574997 1102 96-97 1.2058905044132295 1102 98-99 0.9891901741233546 1102 100-101 0.8260177248669542 1102 102-103 0.6065474544718583 1102 104-105 0.9924947150306416 1102 106-107 1.2558363643206896 1102 108-109 1.17112938032572 1102 110-111 1.0690030197016007 1102 112-113 1.1288520356177685 1102 114-115 1.2324204576395488 1102 116-117 0.8656019122551051 1102 118-119 0.5235625140002611 1102 120-121 0.7757333535352693 1102 122-123 1.1396349312235117 1102 124-125 1.2340519045747769 1103 1 0.7926813016531327 1103 2 0.7388747518176579 1103 3 0.6160022819297701 1103 4 0.7304676561948469 1103 5 1.0777870415884117 1103 6 1.0564882526219428 1103 7 1.283801564160008 1103 8 1.1379595785728043 1103 9 1.7617205647778462 1103 10-11 1.4927037895955877 1103 12-13 1.5209014132357197 1103 14-15 1.3706948820479568 1103 16-17 1.3776830505462456 1103 18-19 1.5343802854744482 1103 20-21 1.7229059010037702 1103 22-23 1.5865162163968094 1103 24-25 1.907643606268877 1103 26-27 1.399380170061228 1103 28-29 1.5413843589406682 1103 30-31 2.0047114848209233 1103 32-33 1.910855619220726 1103 34-35 1.9821996171943503 1103 36-37 1.8180803177874552 1103 38-39 1.9515638857537958 1103 40-41 2.144896960559443 1103 42-43 2.0732969972652953 1103 44-45 1.9514841104545546 1103 46-47 1.8098332000536814 1103 48-49 1.892935270195622 1103 50-51 2.0732717557257523 1103 52-53 1.9024593849107276 1103 54-55 2.1188789770892456 1103 56-57 2.023472239546436 1103 58-59 2.1959302566644183 1103 60-61 2.0836656486452583 1103 62-63 2.2315444444063566 1103 64-65 2.312413442231051 1103 66-67 2.4805214160736817 1103 68-69 2.470172226633121 1103 70-71 2.23484401958833 1103 72-73 2.3135460444911864 1103 74-75 2.2631453554260226 1103 76-77 2.133478607733913 1103 78-79 2.315324177852496 1103 80-81 2.2795408501860663 1103 82-83 2.1792193458780815 1103 84-85 2.391918660617897 1103 86-87 2.2838437357245027 1103 88-89 2.1854771990008146 1103 90-91 2.253327618707246 1103 92-93 2.0423924197949503 1103 94-95 1.9058298316572753 1103 96-97 1.8239919135842584 1103 98-99 2.030260030299015 1103 100-101 2.0235506766829836 1103 102-103 1.8213910498823864 1103 104-105 1.8483477863476665 1103 106-107 1.8623393297748443 1103 108-109 1.8129428166146901 1103 110-111 1.861615203140417 1103 112-113 1.7945442178236775 1103 114-115 1.7199240631416792 1103 116-117 1.7816682961134127 1103 118-119 1.8589320047169018 1103 120-121 1.8528302146364837 1103 122-123 1.9024285180341067 1103 124-125 1.7479917842061816 1104 1 0.7913364483223404 1104 2 0.7508887708984489 1104 3 0.726383020929724 1104 4 0.703727699034296 1104 5 1.0575730030244834 1104 6 1.129263083633095 1104 7 1.4061441427851449 1104 8 1.2045763621608288 1104 9 1.7708257294949519 1104 10-11 1.276664326898505 1104 12-13 1.3732536321355084 1104 14-15 1.574081788593439 1104 16-17 1.3094152500705079 1104 18-19 1.3384286357515265 1104 20-21 1.6761798597673945 1104 22-23 1.6083978484439427 1104 24-25 1.815800430420417 1104 26-27 1.7307979973826377 1104 28-29 1.6583864784905984 1104 30-31 2.0878070424466273 1104 32-33 1.988723477695018 1104 34-35 1.9802814210549649 1104 36-37 1.830029368035703 1104 38-39 2.043902030026924 1104 40-41 2.187253633649057 1104 42-43 2.0806146248830615 1104 44-45 1.7638177677754179 1104 46-47 1.8963401398761874 1104 48-49 1.6161634297646117 1104 50-51 1.993392298083105 1104 52-53 1.7488997706792162 1104 54-55 2.04899619841661 1104 56-57 1.9434003851898787 1104 58-59 2.0445344817756066 1104 60-61 2.1079021341136546 1104 62-63 2.128936746055068 1104 64-65 2.261306220332898 1104 66-67 2.325464287534974 1104 68-69 2.361879702833985 1104 70-71 2.1555636337945963 1104 72-73 2.2847883564750973 1104 74-75 2.3280726187377105 1104 76-77 2.174250495174533 1104 78-79 2.202212012672298 1104 80-81 2.1247126079114445 1104 82-83 2.064815243226306 1104 84-85 2.3135157222653078 1104 86-87 2.2681061444033155 1104 88-89 2.0887244804166087 1104 90-91 2.154657214957588 1104 92-93 1.8583725824644013 1104 94-95 1.8107880641972436 1104 96-97 1.8756865300741268 1104 98-99 1.93963960004033 1104 100-101 2.000907648391774 1104 102-103 1.9277218846433648 1104 104-105 1.9197512685268059 1104 106-107 1.976884491289045 1104 108-109 1.8753860252436318 1104 110-111 1.8935128560237402 1104 112-113 1.894955765245406 1104 114-115 1.8408163775196513 1104 116-117 1.9310678010518103 1104 118-119 1.8966814512945618 1104 120-121 1.861338682030592 1104 122-123 2.048267135814754 1104 124-125 1.8078615477716298 1105 1 0.5207543960527694 1105 2 0.6496162212365562 1105 3 0.4627720239934341 1105 4 0.7307425369111371 1105 5 0.9469311239939238 1105 6 1.0204071442985132 1105 7 1.280149042493285 1105 8 1.1374851748908625 1105 9 1.7550860075158923 1105 10-11 1.423999321471662 1105 12-13 1.5960149590973955 1105 14-15 1.5276738759912334 1105 16-17 1.4398876614845264 1105 18-19 1.6351461729358832 1105 20-21 1.8412769118422787 1105 22-23 1.7411108758858802 1105 24-25 1.9240759955842535 1105 26-27 1.7120910404578638 1105 28-29 1.838313519945224 1105 30-31 2.170679516632063 1105 32-33 2.103152629113481 1105 34-35 2.211094506894508 1105 36-37 1.9178374770554072 1105 38-39 1.932887647099971 1105 40-41 2.0859286376999293 1105 42-43 2.0771457687832466 1105 44-45 2.0057999371319717 1105 46-47 1.9232058606940967 1105 48-49 1.962007834289821 1105 50-51 2.164168807386492 1105 52-53 2.043992597568135 1105 54-55 2.213838268223391 1105 56-57 2.009755941473557 1105 58-59 1.9740579938063547 1105 60-61 1.894409590407328 1105 62-63 1.7719141851546887 1105 64-65 2.0718745317056104 1105 66-67 2.336255183321512 1105 68-69 2.2778938506568913 1105 70-71 2.1073108155171347 1105 72-73 2.3973354901251653 1105 74-75 2.39706699377842 1105 76-77 2.20412133809959 1105 78-79 2.36217060518112 1105 80-81 2.3615499803789 1105 82-83 2.248654555826448 1105 84-85 2.4177598685316966 1105 86-87 2.3510941189605283 1105 88-89 2.2462671698859253 1105 90-91 2.302458192741181 1105 92-93 2.124357279212248 1105 94-95 1.990252921795772 1105 96-97 2.0727423374525777 1105 98-99 2.1491179283256407 1105 100-101 2.2200582209681485 1105 102-103 2.0122619714333823 1105 104-105 1.7100676079358301 1105 106-107 1.8997102735373481 1105 108-109 1.9060816624024959 1105 110-111 2.070822263921116 1105 112-113 2.0598845363181084 1105 114-115 1.9050034599155197 1105 116-117 1.9424520496035065 1105 118-119 2.002371844584573 1105 120-121 2.042536470733424 1105 122-123 2.1976331892281458 1105 124-125 2.0452333830120537 1106 1 0.6636066542288965 1106 2 0.5884265672272413 1106 3 0.5844940779023418 1106 4 0.5190619667288487 1106 5 0.6399144534152477 1106 6 0.8531125815718461 1106 7 0.9940223627350235 1106 8 0.7290682796397903 1106 9 1.5903446075985883 1106 10-11 1.1812120202950425 1106 12-13 1.0263452982594075 1106 14-15 1.2096633821446048 1106 16-17 1.257079303365039 1106 18-19 1.2175780217826215 1106 20-21 1.1422992632344418 1106 22-23 1.1217018484892733 1106 24-25 0.9961103732638534 1106 26-27 0.9894842147545049 1106 28-29 0.9924589424008801 1106 30-31 1.4142222063943635 1106 32-33 1.1798162538171297 1106 34-35 1.2353772194820145 1106 36-37 1.2131013560322614 1106 38-39 1.337551500413742 1106 40-41 1.497492270470353 1106 42-43 1.5674257495291357 1106 44-45 1.5079936129883684 1106 46-47 1.4724668371123855 1106 48-49 1.279488124227207 1106 50-51 1.5774327896822946 1106 52-53 1.3064863507532678 1106 54-55 1.7906229563072813 1106 56-57 1.4671606104650436 1106 58-59 1.5009003806417205 1106 60-61 1.796176609656559 1106 62-63 1.84636640499048 1106 64-65 1.964369710000831 1106 66-67 2.1098856411027924 1106 68-69 1.8765880017248584 1106 70-71 1.8001675576364597 1106 72-73 1.68983686817392 1106 74-75 1.862450103674778 1106 76-77 1.5435830717333658 1106 78-79 1.5276831785219827 1106 80-81 1.6453323571181393 1106 82-83 1.48913011117655 1106 84-85 1.763036363034903 1106 86-87 1.8209030011428098 1106 88-89 1.5214271676566113 1106 90-91 1.6477273117287368 1106 92-93 1.6191367411106725 1106 94-95 1.4852100904174037 1106 96-97 1.4098371014721813 1106 98-99 1.394772592364113 1106 100-101 1.2013435289348102 1106 102-103 0.972784138190768 1106 104-105 1.135810900984449 1106 106-107 1.1334925175790254 1106 108-109 1.070059989619505 1106 110-111 1.019060216201467 1106 112-113 0.8124471113963807 1106 114-115 0.8728932421847446 1106 116-117 1.2096015121752934 1106 118-119 0.9659154422450733 1106 120-121 0.9683386136077168 1106 122-123 1.132030554360668 1106 124-125 1.1779360830124261 1107 1 0.5648593230886867 1107 2 0.65840421208366 1107 3 0.5109398397259994 1107 4 0.42119464010224306 1107 5 0.4760362366552009 1107 6 0.8386588270876452 1107 7 0.9577518762355339 1107 8 0.9042282498494814 1107 9 1.5878692302527355 1107 10-11 1.0841419145722213 1107 12-13 1.4168530890427675 1107 14-15 1.2296034632591955 1107 16-17 1.311908587268661 1107 18-19 1.3512846773862393 1107 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2216 122-123 -5.4065195950320835 2216 124-125 -5.265061036947923 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 179291.0 3 59917.0 4 28880.0 5 23399.0 6 23549.0 7 26993.0 8 30413.0 9 32164.0 10 33898.0 11 36386.0 12 39317.0 13 42034.0 14 42751.0 15 35257.0 16 26269.0 17 22899.0 18 20428.0 19 18800.0 20 18489.0 21 19848.0 22 24359.0 23 33252.0 24 45895.0 25 60472.0 26 73909.0 27 84201.0 28 95136.0 29 106834.0 30 119465.0 31 135617.0 32 159579.0 33 188855.0 34 228550.0 35 289437.0 36 408632.0 37 535641.0 38 194722.0 39 882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.90144510669288 20.093009601583 14.32740226582116 24.678143025902962 2 15.540378404884034 20.884087668300335 38.7488146473634 24.826719279452224 3 18.673028011346656 25.12920071508733 29.81871859509026 26.379052678475755 4 12.789173836658927 17.638223335383316 36.71212126776443 32.86048156019333 5 13.118299580985896 38.59176296095781 34.28536383169506 14.004573626361234 6 31.552576771916645 37.71423753684708 17.023731577869928 13.709454113366348 7 27.345900616930656 32.894468497779066 21.18457681817682 18.57505406711346 8 25.801258734160083 36.24858307814641 19.41639738102086 18.533760806672646 9 25.551119156783457 16.25605540234941 20.134840205051855 38.05798523581527 10-11 24.67814828730886 27.15410096316186 27.34368798715878 20.82406276237049 12-13 25.44046124572665 24.312541521025853 27.954041229462252 22.292956003785253 14-15 22.29286503516311 25.650282405982516 25.886338588566467 26.170513970287914 16-17 22.14487466156232 29.111456482938287 26.385766858225367 22.35790199727403 18-19 22.077510461430876 27.36909112442053 28.8048872646883 21.748511149460292 20-21 22.585531227331078 26.65710738750399 28.96420698518241 21.793154399982516 22-23 22.248079721178897 26.446342164924037 28.930791008245073 22.37478710565199 24-25 22.168987299473724 26.737471416836634 28.810686613477802 22.282854670211837 26-27 22.08011168945099 27.02689860857695 28.70149310760078 22.191496594371284 28-29 21.93658588511805 26.937625703463546 28.989952182455337 22.135836228963065 30-31 22.026436189943656 26.64359812806747 28.837632989166302 22.492332692822572 32-33 21.842665428233623 26.767847661456617 28.822231001035693 22.567255909274063 34-35 22.35140107856614 26.76848894998414 28.59064537732191 22.289464594127807 36-37 22.39546815667789 26.56726741685128 28.511071104648796 22.526193321822035 38-39 22.13473592137822 26.813908145916564 28.757190573586456 22.294165359118757 40-41 22.284945448229113 26.590759641728017 28.725147729105593 22.39914718093728 42-43 22.28765501339814 26.694562663352873 28.416808430664787 22.6009738925842 44-45 22.363963022005965 26.80007878457452 28.222219308452466 22.61373888496705 46-47 22.45224676567198 27.061303419881916 27.74706752661636 22.739382287829745 48-49 22.59733942774645 27.208048685751184 27.42470394419157 22.769907942310795 50-51 22.458035426658398 27.139117081060892 27.382515273564508 23.020332218716202 52-53 22.827309103321113 27.11965846067909 27.26546769984163 22.787564736158167 54-55 22.4790581219616 26.99000959299309 27.323865517389862 23.207066767655448 56-57 22.481551437374282 27.41622559912272 27.293679292141398 22.808543671361598 58-59 22.58493390305349 27.0890259880423 27.354434521917103 22.971605586987103 60-61 22.55763558743747 27.141342536980957 27.474030148713354 22.826991726868222 62-63 22.54062541547479 27.142637402578874 27.490497743553348 22.826239438392992 64-65 22.368393395649232 26.88947695924272 27.42508839041627 23.317041254691784 66-67 22.207478583952458 27.369433696650308 27.212732974012987 23.210354745384247 68-69 22.33141239710227 27.12885123652104 27.468748951389887 23.0709874149868 70-71 22.21566298048874 27.134845195472142 27.548204317105863 23.10128750693326 72-73 22.42752323762694 27.09107603453681 27.291616169511297 23.189784558324956 74-75 22.339115637770092 27.263175570930482 27.130091345774925 23.267617445524504 76-77 22.389832171673394 27.144479328393434 27.323521964198804 23.142166535734365 78-79 22.418094256002558 27.316831356519337 27.118571900282063 23.146502487196038 80-81 22.289382461505795 27.485523742950797 27.13392879927465 23.091164996268763 82-83 22.432827883505922 27.354816642849165 27.080144664522905 23.132210809122007 84-85 22.3093351349629 27.183293908587792 26.892634513857498 23.61473644259181 86-87 22.426037154766977 27.18370892922954 27.159261664404756 23.23099225159873 88-89 22.363914958604113 27.225115167155572 27.01124841281232 23.39972146142799 90-91 22.250278767863666 27.442177785729484 27.156466279486335 23.151077166920516 92-93 22.427579540478224 27.138564587311027 27.181411590939277 23.252444281271476 94-95 22.25570639254702 27.04258765367486 27.162797924363684 23.538908029414436 96-97 22.46125470151073 27.043663547153702 27.13179682621502 23.36328492512055 98-99 22.022673569402386 26.94855657254358 27.0978056744548 23.930964183599237 100-101 21.994242561899227 26.982104795073496 27.371305693231584 23.652346949795692 102-103 22.31437595952028 26.81627698869985 27.251045682364932 23.618301369414937 104-105 22.176004931181147 26.875599902098234 27.282165749495547 23.666229417225072 106-107 21.944314226962845 26.93857956656259 27.298238292586813 23.818867913887747 108-109 22.143801959102973 26.914825913125323 26.96892273586398 23.972449391907723 110-111 21.81190146380321 27.037788187358352 27.214586571641057 23.935723777197378 112-113 22.032941980596192 26.966146395079882 27.209391901741952 23.791519722581974 114-115 21.79604705144574 27.218140793409457 26.965068395811432 24.020743759333367 116-117 21.732690995426374 27.03806937700554 27.180396004985308 24.048843622582776 118-119 21.79656781089419 27.184954413572882 26.774283917290102 24.244193858242827 120-121 21.60035991975966 27.237852708111394 27.01033683684559 24.15145053528336 122-123 21.655811545547802 27.223476387080332 26.90257404256591 24.218138024805956 124-125 21.688901183824658 27.15196977701752 26.845823056416695 24.313305982741124 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 248.0 1 213.5 2 270.5 3 402.0 4 366.0 5 327.5 6 561.0 7 1233.5 8 2064.5 9 2530.0 10 2199.5 11 1661.0 12 1634.0 13 1823.5 14 2061.5 15 2413.0 16 2761.0 17 3112.5 18 3613.5 19 4226.5 20 4958.0 21 5849.5 22 7081.0 23 8774.5 24 11028.5 25 14184.0 26 18826.0 27 23872.5 28 28632.5 29 34025.5 30 40656.0 31 48229.0 32 56453.0 33 64358.5 34 71561.5 35 77823.5 36 85390.0 37 94449.5 38 100666.5 39 103524.5 40 105784.5 41 111218.0 42 116434.5 43 120645.5 44 125541.5 45 129390.0 46 129411.5 47 128476.0 48 132144.0 49 133917.0 50 132849.5 51 130539.5 52 125793.0 53 123134.5 54 120205.0 55 111570.0 56 101445.5 57 92093.5 58 82943.5 59 73320.0 60 61850.5 61 50711.0 62 41374.5 63 32206.5 64 24643.0 65 19022.0 66 14291.5 67 11442.5 68 8422.5 69 5686.5 70 4103.5 71 3070.0 72 2389.0 73 1883.0 74 1467.5 75 1128.5 76 746.0 77 429.5 78 255.5 79 157.0 80 114.5 81 76.5 82 49.0 83 29.5 84 20.0 85 14.5 86 11.5 87 7.0 88 6.0 89 4.5 90 2.5 91 1.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10644537308045861 2 1.973821487584663E-4 3 0.0 4 0.18472149378810182 5 0.0 6 1.6918469893582823E-4 7 0.03634651282138043 8 0.0 9 0.0 10-11 7.754298701225461E-4 12-13 6.767387957433129E-4 14-15 5.216528217188037E-4 16-17 0.0045538881463560435 18-19 0.0016918469893582825 20-21 0.00359517485238635 22-23 0.0016918469893582825 24-25 0.00532931801647859 26-27 0.00446929579688813 28-29 0.00359517485238635 30-31 8.882196694130983E-4 32-33 8.459234946791411E-5 34-35 0.0012688852420187117 36-37 0.001367576316397945 38-39 6.62640070831994E-4 40-41 0.0018046367886488345 42-43 9.305158441470553E-4 44-45 0.0011419967178168406 46-47 3.1017194804901845E-4 48-49 7.04936245565951E-5 50-51 0.039645614450629085 52-53 1.5508597402450922E-4 54-55 0.13457232927854007 56-57 1.5508597402450922E-4 58-59 1.409872491131902E-5 60-61 0.0 62-63 7.190349704772701E-4 64-65 0.058072647909723045 66-67 0.04976849893695614 68-69 0.0016636495395356446 70-71 0.0030735220306675466 72-73 0.07023984750819136 74-75 0.0047794677449371475 76-77 0.0016354520897130063 78-79 0.0016072546398903684 80-81 2.678757733150614E-4 82-83 2.255795985811043E-4 84-85 2.819744982263804E-5 86-87 3.1017194804901845E-4 88-89 0.0014944648405998161 90-91 2.9607322313769945E-4 92-93 0.0022839934356336813 94-95 0.003778458276233497 96-97 0.0024390794096581904 98-99 0.0 100-101 0.0014380699409545401 102-103 0.0014521686658658592 104-105 2.255795985811043E-4 106-107 7.04936245565951E-5 108-109 2.819744982263804E-5 110-111 2.114808736697853E-4 112-113 8.459234946791411E-5 114-115 1.1278979929055215E-4 116-117 0.0 118-119 6.90837520654632E-4 120-121 0.002086611286875215 122-123 3.947642975169326E-4 124-125 0.006894276481635001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3546420.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.46601383103457 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8792416307479 41.104265963490164 2 11.558053273074743 12.359260723160974 3 4.481234636852052 7.187812592221291 4 2.25032724337563 4.8126411007470145 5 1.286402818359658 3.4389415439349666 6 0.8028850669125281 2.575623845536581 7 0.5314493401686424 1.9890134440365586 8 0.3845863600751013 1.644983971760209 9 0.27898590280101926 1.3424637724040671 >10 1.4000828950226691 13.277991429825494 >50 0.09205668394691963 3.3992334026239233 >100 0.05070870665669929 4.852956559492578 >500 0.0034468687652945093 1.2273160260055946 >1k 4.308585930478424E-4 0.3900908011054212 >5k 1.077146482619606E-4 0.3974048236551206 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7280 0.20527743470880494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6521 0.18387557029342266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4093 0.1154121621240575 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.8197449822638042E-5 0.0 12-13 0.0 0.0 0.0 4.229617473395706E-5 0.0 14-15 0.0 0.0 0.0 8.459234946791413E-5 0.0 16-17 0.0 0.0 0.0 1.9738214875846628E-4 2.8197449822638042E-5 18-19 1.4098724911319021E-5 1.4098724911319021E-5 0.0 2.2557959858110434E-4 2.8197449822638042E-5 20-21 2.8197449822638042E-5 2.8197449822638042E-5 0.0 3.9476429751693256E-4 2.8197449822638042E-5 22-23 2.8197449822638042E-5 2.8197449822638042E-5 0.0 7.190349704772701E-4 2.8197449822638042E-5 24-25 2.8197449822638042E-5 4.229617473395706E-5 0.0 0.0011137992679942027 2.8197449822638042E-5 26-27 2.8197449822638042E-5 5.6394899645276084E-5 0.0 0.001381675041309264 4.229617473395706E-5 28-29 2.8197449822638042E-5 8.459234946791413E-5 0.0 0.002143006186520491 5.6394899645276084E-5 30-31 5.6394899645276084E-5 8.459234946791413E-5 0.0 0.004441098347065491 5.6394899645276084E-5 32-33 5.6394899645276084E-5 8.459234946791413E-5 0.0 0.009756317638632762 5.6394899645276084E-5 34-35 5.6394899645276084E-5 8.459234946791413E-5 0.0 0.017397826540567672 5.6394899645276084E-5 36-37 5.6394899645276084E-5 8.459234946791413E-5 0.0 0.02932534781554356 5.6394899645276084E-5 38-39 5.6394899645276084E-5 8.459234946791413E-5 0.0 0.048894377992454365 5.6394899645276084E-5 40-41 5.6394899645276084E-5 8.459234946791413E-5 0.0 0.07210087919648547 5.6394899645276084E-5 42-43 7.049362455659511E-5 8.459234946791413E-5 0.0 0.09615330389519572 5.6394899645276084E-5 44-45 8.459234946791413E-5 8.459234946791413E-5 0.0 0.12581702110861093 5.6394899645276084E-5 46-47 8.459234946791413E-5 8.459234946791413E-5 0.0 0.16339012299727612 5.6394899645276084E-5 48-49 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.20337410684577686 5.6394899645276084E-5 50-51 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.248898889584426 5.6394899645276084E-5 52-53 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.2927882202333621 5.6394899645276084E-5 54-55 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.3425567191703182 8.459234946791413E-5 56-57 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.402250720444843 8.459234946791413E-5 58-59 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.46762650785862925 8.459234946791413E-5 60-61 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.5419267881412805 8.459234946791413E-5 62-63 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.6180881001122258 8.459234946791413E-5 64-65 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.6973088353889274 1.1278979929055217E-4 66-67 1.1278979929055217E-4 8.459234946791413E-5 0.0 0.7891479294612596 1.1278979929055217E-4 68-69 1.409872491131902E-4 8.459234946791413E-5 0.0 0.8983566526243367 1.1278979929055217E-4 70-71 1.409872491131902E-4 8.459234946791413E-5 0.0 1.0225946165428799 1.1278979929055217E-4 72-73 1.6918469893582825E-4 8.459234946791413E-5 0.0 1.1509917043102622 1.1278979929055217E-4 74-75 1.6918469893582825E-4 8.459234946791413E-5 0.0 1.2819970561862384 1.1278979929055217E-4 76-77 1.6918469893582825E-4 8.459234946791413E-5 0.0 1.4300054703052656 1.1278979929055217E-4 78-79 1.6918469893582825E-4 8.459234946791413E-5 0.0 1.6073956271394816 1.1278979929055217E-4 80-81 1.9738214875846628E-4 8.459234946791413E-5 0.0 1.804072839652382 1.1278979929055217E-4 82-83 1.9738214875846628E-4 1.1278979929055217E-4 0.0 2.011126713700013 1.1278979929055217E-4 84-85 1.9738214875846628E-4 1.1278979929055217E-4 0.0 2.223862373886906 1.1278979929055217E-4 86-87 1.9738214875846628E-4 1.1278979929055217E-4 0.0 2.4501187112637535 1.1278979929055217E-4 88-89 2.2557959858110434E-4 1.1278979929055217E-4 0.0 2.7015553713322165 1.1278979929055217E-4 90-91 2.2557959858110434E-4 1.1278979929055217E-4 0.0 2.98259935371445 1.1278979929055217E-4 92-93 2.2557959858110434E-4 1.1278979929055217E-4 0.0 3.292432932365597 1.1278979929055217E-4 94-95 2.2557959858110434E-4 1.1278979929055217E-4 0.0 3.610345080390929 1.1278979929055217E-4 96-97 2.2557959858110434E-4 1.1278979929055217E-4 0.0 3.9324727471647467 1.1278979929055217E-4 98-99 2.8197449822638045E-4 1.1278979929055217E-4 0.0 4.283968057928841 1.1278979929055217E-4 100-101 3.1017194804901845E-4 1.1278979929055217E-4 0.0 4.68542924977865 1.1278979929055217E-4 102-103 3.524681227829755E-4 1.1278979929055217E-4 0.0 5.121023454638762 1.1278979929055217E-4 104-105 4.793566469848467E-4 1.1278979929055217E-4 0.0 5.55592682197822 1.1278979929055217E-4 106-107 5.075540968074848E-4 1.1278979929055217E-4 0.0 6.003490844288042 1.1278979929055217E-4 108-109 5.075540968074848E-4 1.2688852420187117E-4 0.0 6.475431561969536 1.1278979929055217E-4 110-111 5.075540968074848E-4 1.409872491131902E-4 0.0 6.99260098916654 1.1278979929055217E-4 112-113 5.075540968074848E-4 1.409872491131902E-4 0.0 7.546821865430491 1.1278979929055217E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5845 0.0 67.17491 1 GTATCAA 10470 0.0 52.148293 1 ATCAACG 13225 0.0 39.477737 3 TCAACGC 13275 0.0 39.104565 4 TATCAAC 13925 0.0 38.734432 2 CAACGCA 13490 0.0 38.367752 5 AACGCAG 13635 0.0 37.217995 6 ACGCAGA 16200 0.0 31.104801 7 CGCAGAG 16375 0.0 31.087524 8 GTACATG 12200 0.0 29.150915 1 TACATGG 12460 0.0 27.312529 2 GCAGAGT 18520 0.0 26.459394 9 ACATGGG 11950 0.0 26.433325 3 GAGTACT 11150 0.0 25.843746 12-13 CAGAGTA 17480 0.0 24.650944 10-11 GTGGTAT 2920 0.0 24.318111 1 AGAGTAC 16745 0.0 23.99257 10-11 GTACTTT 12365 0.0 23.592909 14-15 AGTACTT 12100 0.0 22.90536 12-13 TGGTATC 3095 0.0 21.182434 2 >>END_MODULE