FastQCFastQC Report
Thu 26 May 2016
SRR1364472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548594
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64600.045.2473561
GGTATCA39150.039.2312551
ATCAACG74700.038.391223
TCAACGC75850.038.0773664
CAACGCA76650.037.679215
AACGCAG77000.036.717276
TATCAAC81600.035.9469682
GTACATG84650.033.545611
ACGCAGA87000.032.0186237
TACATGG83600.031.5996442
CGCAGAG88750.030.248328
ACATGGG85350.029.4180873
GCAGAGT98150.025.5931519
ACGCATA3302.910383E-1123.44898
AGAGTAC80900.023.06063510-11
GAGTACT41950.023.04427112-13
CATGGGG47800.021.5525654
CAGAGTA94350.021.16083710-11
TATACCG2556.341579E-721.0171855
GAGTACA68050.020.2957041
Produced by FastQC (version 0.11.5)