FastQCFastQC Report
Thu 26 May 2016
SRR1364472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548594
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45640.1790791314740598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.1705253955710482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.051.196091
GTATCAA83000.043.7833481
ATCAACG103350.033.9633643
TATCAAC108300.033.402822
TCAACGC104100.033.14624
CAACGCA105850.032.3227925
AACGCAG108400.031.617326
GTACATG82100.029.0001011
TACATGG83800.027.6648642
ACGCAGA126700.026.7224377
CGCAGAG129200.026.5131728
ACATGGG81900.026.2681313
GAGTACT81150.024.80735412-13
GCAGAGT138850.023.8567379
AGAGTAC118000.023.63747610-11
GTACTTT88850.022.92566514-15
AGTACTT86900.022.54996712-13
CAGAGTA133900.022.07425710-11
CATGGGG45050.021.2979094
ACTTTTT116050.018.11638816-17
Produced by FastQC (version 0.11.5)